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https://www.readbyqxmd.com/read/28445031/importance-of-loop-l1-dynamics-for-substrate-capture-and-catalysis-in-pseudomonas-aeruginosa-d-arginine-dehydrogenase
#1
Daniel Ouedraogo, Michael Souffrant, Sheena Vasquez, Donald Hamelberg, Giovanni Gadda
Mobile loops located at the active site entrance in enzymes often participate in conformational changes required to shield the reaction from bulk solvent, to control substrate access to the active site, and to position residues for substrate binding and catalysis. In D-arginine dehydrogenase from Pseudomonas aeruginosa (PaDADH) previous crystallographic data suggested that residues 45-47 in the FAD-binding domain and 50-56 in the substrate-binding domain in loop L1 could adopt two distinct conformations. In this study, we have used molecular dynamics, kinetics, and fluorescence spectroscopy on the S45A and A46G enzyme variants of PaDADH to investigate the impact of mutations of loop L1 on the catalytic function of the enzyme...
April 26, 2017: Biochemistry
https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#2
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444395/effects-of-n-acetyl-l-cysteine-on-the-membrane-vesicle-release-and-growth-of-respiratory-pathogens
#3
Charlotte Volgers, Birke J Benedikter, Gert E Grauls, Pauline H M Hellebrand, Paul H M Savelkoul, Frank R M Stassen
Bacterial infections contribute to the disease progression of chronic obstructive pulmonary disease (COPD) by stimulating mucus production in the airways. This increased mucus production and other symptoms are often alleviated when patients are treated with mucolytics such as N-acetyl-L-cysteine (NAC). Moreover, NAC has been suggested to inhibit bacterial growth. Bacteria can release membrane vesicles in response to stress and recent studies report a role for these pro-inflammatory membrane vesicles (MVs) in the pathogenesis of airways disease...
April 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28444073/antibacterial-activity-of-standard-and-n-doped-titanium-dioxide-coated-endotracheal-tubes-an-in-vitro-study
#4
Valentina Caratto, Lorenzo Ball, Elisa Sanguineti, Angelo Insorsi, Iacopo Firpo, Stefano Alberti, Maurizio Ferretti, Paolo Pelosi
Objective: The aim of this study was to assess the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa of two nanoparticle endotracheal tube coatings with visible light-induced photocatalysis. Methods: Two types of titanium dioxide nanoparticles were tested: standard anatase (TiO2) and N-doped TiO2 (N-TiO2). Nanoparticles were placed on the internal surface of a segment of commercial endotracheal tubes, which were loaded on a cellulose acetate filter; control endotracheal tubes were left without a nanoparticle coating...
January 2017: Revista Brasileira de Terapia Intensiva
https://www.readbyqxmd.com/read/28443385/pseudomonas-aeruginosa-prevalence-antibiotic-resistance-and-antimicrobial-use-in-chinese-burn-wards-from-2007-to-2014
#5
Yi Dou, Jingning Huan, Feng Guo, Zengding Zhou, Yan Shi
Objective To assess the application of antibacterial agents, alongside pathogen prevalence and Pseudomonas aeruginosa drug resistance, with the aim of understanding the impact of inappropriate antibacterial use. Methods This retrospective study assessed bacteria from wounds, catheters, blood, faeces, urine and sputum of hospitalized patients in burn wards between 2007 and 2014. The intensity of use of antibacterial agents and resistance of P. aeruginosa to common anti-Gram-negative antibiotics were measured...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28443279/analogs-of-the-frog-skin-antimicrobial-peptide-temporin-1tb-exhibit-a-wider-spectrum-of-activity-and-a-stronger-antibiofilm-potential-as-compared-to-the-parental-peptide
#6
Lucia Grassi, Giuseppantonio Maisetta, Giuseppe Maccari, Semih Esin, Giovanna Batoni
The frog skin-derived peptide Temporin 1Tb (TB) has gained increasing attention as novel antimicrobial agent for the treatment of antibiotic-resistant and/or biofilm-mediated infections. Nevertheless, such a peptide possesses a preferential spectrum of action against Gram-positive bacteria. In order to improve the therapeutic potential of TB, the present study evaluated the antibacterial and antibiofilm activities of two TB analogs against medically relevant bacterial species. Of the two analogs, TB_KKG6A has been previously described in the literature, while TB_L1FK is a new analog designed by us through statistical-based computational strategies...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28442545/peroxisome-proliferator-activated-receptor-%C3%AE-agonists-attenuate-biofilm-formation-by-pseudomonas-aeruginosa
#7
Brahmchetna Bedi, Nicholas M Maurice, Vincent T Ciavatta, K Sabrina Lynn, Zhihong Yuan, Samuel A Molina, Myungsoo Joo, William R Tyor, Joanna B Goldberg, Michael H Koval, C Michael Hart, Ruxana T Sadikot
Pseudomonas aeruginosa is a significant contributor to recalcitrant multidrug- resistant infections, especially in immunocompromised and hospitalized patients. The pathogenic profile of P. aeruginosa is related to its ability to secrete a variety of virulence factors and to promote biofilm formation. Quorum sensing (QS) is a mechanism wherein P. aeruginosa secretes small diffusible molecules, specifically acyl homo serine lactones, such as N-(3-oxo-dodecanoyl)-l-homoserine lactone (3O-C12-HSL), that promote biofilm formation and virulence via interbacterial communication...
April 25, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28441606/growth-inhibition-and-antibiofilm-potential-of-ag-nanoparticles-coated-with-lectin-an-arthropod-immune-molecule
#8
Sangily Jayanthi, Sathappan Shanthi, Baskaralingam Vaseeharan, Narayanan Gopi, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
Lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. Here, lectin was purified from the haemolymph of blue swimmer crab Portunus pelagicus, using mannose-coupled sepharose CL-4B affinity column chromatography. In SDS-PAGE analysis, lectin showed a molecular mass of approximately 155kDa. The synthesis of lectin-coated silver nanoparticles (lectin-coated AgNPs) was confirmed by UV-Vis spectroscopy, XRD, FTIR, SEM, TEM, SAED, and EDX analysis...
April 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28440922/pseudomonas-aeruginosa-and-post-tracheotomy-bacterial-respiratory-tract-infection-readmissions
#9
Christopher J Russell, Tamara D Simon, Mary R Mamey, Christopher J L Newth, Michael N Neely
OBJECTIVE: Identify risk factors for readmission due to a bacterial tracheostomy-associated respiratory tract infection (bTARTI) within 12 months of discharge after tracheotomy. DESIGN/METHODS: We performed a retrospective cohort study of 240 children who underwent tracheotomy and were discharged with tracheotsomy in place between January 1, 2005 and June 30, 2013. Children with prolonged total or post-tracheotomy length of stay (LOS), less than 12 months of follow-up, or who died during the index hospitalization were excluded...
April 25, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28440853/antibiotic-strategies-for-eradicating-pseudomonas-aeruginosa-in-people-with-cystic-fibrosis
#10
REVIEW
Simon C Langton Hewer, Alan R Smyth
BACKGROUND: Respiratory tract infection with Pseudomonas aeruginosa occurs in most people with cystic fibrosis. Once chronic infection is established, Pseudomonas aeruginosa is virtually impossible to eradicate and is associated with increased mortality and morbidity. Early infection may be easier to eradicate.This is an update of a Cochrane review first published in 2003, and previously updated in 2006, 2009 and 2014. OBJECTIVES: To determine whether antibiotic treatment of early Pseudomonas aeruginosa infection in children and adults with cystic fibrosis eradicates the organism, delays the onset of chronic infection, and results in clinical improvement...
April 25, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28440622/understanding-carbapenem-translocation-through-occd3-opdp-of-pseudomonas-aeruginosa
#11
Gowrishankar Soundararajan, Satya Prathyusha Bhamidimarri, Mathias Winterhalter
Pseudomonas aeruginosa utilizes a plethora of substrate specific channels for the uptake of small nutrients. OccD3 (OpdP or PA4501) is an OprD like arginine uptake channel of P. aeruginosa whose role has been implicated in carbapenem uptake. To understand the mechanism of selective permeation we reconstituted single OccD3 channels in planar lipid bilayer and characterized the interaction with Imipenem and Meropenem analyzing the ion current fluctuation in presence of substrates. We performed point mutations in the constriction region of OccD3 to understand the binding and translocation of antibiotic in OccD3...
April 25, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28440605/-carbapenem-resistance-in-pseudomonas-aeruginosa-isolated-from-urine-cultures-prevalence-and-risk-factors
#12
J Álvarez-Otero, J L Lamas-Ferreiro, L González-González, I Rodríguez-Conde, M J Fernández-Soneira, A Arca-Blanco, J R Bermúdez-Sanjurjo, J de la Fuente-Aguado
OBJECTIVE: Pseudomonas aeruginosa is a non-fermentative gram-negative bacillus with a great ability to develop resistance to multiple antibiotics, including carbapenems, which is a growing problem worldwide. The aim of this study was to analyse the prevalence of carbapenem-resistant P. aeruginosa (CRPA) in urine cultures and to determine the risk factors associated with the development of carbapanem resistance. METHODS: Positive urine cultures to P. aeruginosa between September 2012 and September 2014 were identified...
April 25, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28440203/antimicrobial-biofilm-inhibitory-and-anti-infective-activity-of-metallic-nanoparticles-against-multi-drug-resistant-mrsa-and-pseudomonas-aeruginosa-pa01
#13
Jamuna Bai Ashwathanarayan, Ravishankar Rai Vittal
BACKGROUND: The emergence of drug resistant pathogens is a major concern to the scientific community. Novel approaches such as the use of functionalized nanomaterials with antimicrobial activity is required to treat infectious diseases. OBJECTIVE: In the present study, the metallic nanoparticles (iron, gold, zinc oxide and copper oxide) were evaluated for the antimicrobial, biofilm inhibitory and anti-infective activity against human pathogens methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa PA01...
April 24, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28440108/microbiology-of-the-upper-and-lower-airways-in-pediatric-cystic-fibrosis-patients
#14
Lindsay Sobin, Kosuke Kawai, Alexandria L Irace, Ozgul Gergin, Michael Cunningham, Gregory S Sawicki, Eelam A Adil
Objective To evaluate the microbiology of the upper and lower airways in pediatric cystic fibrosis (CF) patients who underwent sinus surgery. Study Design Retrospective case series with chart review. Setting Tertiary care children's hospital. Subjects and Methods A total of 201 paired sinus and pulmonary cultures from 105 CF patients were identified between 1996 and 2014. Demographics and culture results were analyzed. Results The mean age of patients was 11.2 ± 5.4 years (range, 1-27 years), and approximately one-half were female...
April 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28439630/myriocin-treatment-of-cf-lung-infection-and-inflammation-complex-analyses-for-enigmatic-lipids
#15
Anna Caretti, Michele Vasso, Fabiola Tecla Bonezzi, Andrea Gallina, Marco Trinchera, Alice Rossi, Raffaella Adami, Josefina Casas, Monica Falleni, Delfina Tosi, Alessandra Bragonzi, Riccardo Ghidoni, Cecilia Gelfi, Paola Signorelli
Our aim was to use quantitative and qualitative analyses to gain further insight into the role of ceramide in cystic fibrosis (CF). Sphingolipid ceramide is a known inflammatory mediator, and its accumulation in inflamed lung has been reported in different types of emphysema, chronic obstructive pulmonary disease and CF. CF is caused by a mutation of the chloride channel and associated with hyperinflammation of the respiratory airways and high susceptibility to ongoing infections. We have previously demonstrated that de novo ceramide synthesis is enhanced in lung inflammation and sustains Pseudomonas aeruginosa pulmonary infection in a CF murine model...
April 24, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28439602/antibiogram-profile-of-septic-meningitis-among-children-in-duhok-iraq
#16
Abdulrahman T Saadi, Nadir A Garjees, Aveen H Rasool
  To determine the prevalence of bacteria in the cerebrospinal fluid (CSF), and the antibiogram profile in pediatric patients with suspected meningitis. Methods: This descriptive study was conducted between January 2014 and January 2016 in the Hevi Paediatric Teaching Hospital in Duhok, Iraq. The CSF samples were withdrawn from 432 pediatric patients suspected of meningitis. The samples were cultured, and antibiotic sensitivity tests were performed.  Results: There were 33 (7.6%) culture positive cases among 432 CSF samples...
May 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28439261/interaction-of-bacterial-membrane-vesicles-with-specific-species-and-their-potential-for-delivery-to-target-cells
#17
Yosuke Tashiro, Yusuke Hasegawa, Masaki Shintani, Kotaro Takaki, Moriya Ohkuma, Kazuhide Kimbara, Hiroyuki Futamata
Membrane vesicles (MVs) are secreted from a wide range of microbial species and transfer their content to other cells. Although MVs play critical roles in bacterial communication, whether MVs selectively interact with bacterial cells in microbial communities is unclear. In this study, we investigated the specificity of the MV-cell interactions and evaluated the potential of MVs to target bacterial cells for delivery. MV association with bacterial cells was examined using a fluorescent membrane dye to label MVs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28439038/pseudomonas-aeruginosa-derived-rhamnolipids-and-other-detergents-modulate-colony-morphotype-and-motility-in-the-burkholderia-cepacia-complex
#18
Steve P Bernier, Courtney Hum, Xiang Li, George A O'Toole, Nathan Magarvey, Michael G Surette
Competitive interactions mediated by released chemicals (e.g. toxins) are prominent in multispecies communities, but their effect at subinhibitory concentrations on susceptible bacteria is poorly understood. Although Pseudomonas aeruginosa and species of the Burkholderia cepacia complex (Bcc) can exist together as a co-infection in cystic fibrosis airways, P. aeruginosa toxins can kill Bcc species in vitro Consequently, these bacteria become an ideal in vitro model system to study the impact of sublethal levels of toxins on the biology of typical susceptible bacteria such as the Bcc when exposed to P...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438998/cas1-and-the-csy-complex-are-opposing-regulators-of-cas2-3-nuclease-activity
#19
MaryClare F Rollins, Saikat Chowdhury, Joshua Carter, Sarah M Golden, Royce A Wilkinson, Joseph Bondy-Denomy, Gabriel C Lander, Blake Wiedenheft
The type I-F CRISPR adaptive immune system in Pseudomonas aeruginosa (PA14) consists of two CRISPR loci and six CRISPR-associated (cas) genes. Type I-F systems rely on a CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease (i.e., Cas2/3) for target degradation. In most type I systems, Cas2 and Cas3 are separate proteins involved in adaptation and interference, respectively. However, in I-F systems, these proteins are fused into a single polypeptide...
April 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28438946/potassium-iodide-potentiates-antimicrobial-photodynamic-inactivation-mediated-by-rose-bengal-in-vitro-and-in-vivo-studies
#20
Xiang Wen, Xiaoshen Zhang, Grzegorz Szewczyk, Ahmed El-Hussein, Ying-Ying Huang, Tadeusz Sarna, Michael R Hamblin
Rose Bengal (RB) is a halogenated xanthene dye that has been used to mediate antimicrobial photodynamic inactivation for several years. While highly active against Gram-positive bacteria, RB is largely inactive in killing Gram-negative bacteria. We have discovered that addition of the non-toxic salt potassium iodide (100mM) potentiates green light (540nm)-mediated killing by up to six extra logs with Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive methicillin resistant Staphylococcus aureus, and fungal yeast Candida albicans...
April 24, 2017: Antimicrobial Agents and Chemotherapy
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