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https://www.readbyqxmd.com/read/27919520/the-nanjing-glycated-hemoglobin-standardization-program-2012-to-2015
#1
Kui Zhang, Yan Wang, Hongxia Wei, Zhiye Xu
PURPOSE: This study aims to investigate glycosylated hemoglobin (HbA1c), between-laboratory imprecision (%CV), and pass rates from 2012 to 2015 in Nanjing to provide evidence for improving the HbA1c measurement. METHODS: This was a retrospective, descriptive analysis of HbA1c levels obtained from participating hospitals in Nanjing from 2012 to 2015. The HbA1c levels of fresh whole blood samples taken from healthy and diabetic adults were determined within 24 hours...
December 2, 2016: Clinical Therapeutics
https://www.readbyqxmd.com/read/27916603/oxidative-stress-in-sepsis-possible-production-of-free-radicals-through-an-erythrocyte-mediated-positive-feedback-mechanism
#2
Yanaihara Pichemel Amorim de Oliveira, Lain Carlos Pontes-de-Carvalho, Ricardo David Couto, Alberto Augusto Noronha-Dutra
BACKGROUND: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species (ROS) in the early stages of sepsis. This study aimed at investigating the production of ROS during the inflammatory response in patients with sepsis. METHODS: ROS production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals...
December 1, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27895055/role-of-agents-for-reversing-the-effects-of-target-specific-oral-anticoagulants
#3
REVIEW
Tanya R Riley, Mary L Gauthier-Lewis, Chelsea K Sanchez, Janine S Douglas
PURPOSE: The available clinical data on target-specific oral anticoagulant (TSOAC) reversal agents that are currently in development or have been approved by the Food and Drug Administration (FDA) are reviewed. SUMMARY: The development of TSOACs such as dabigatran, rivaroxaban, edoxaban, and apixaban has presented benefits and new challenges. One of the main challenges associated with the use of TSOACs is the lack of suitable agent-specific reversal agents. Several treatment options for the management of life-threatening bleeding events associated with TSOAC use, such as fresh frozen plasma, prothrombin complex concentrates, and recombinant coagulation factor VIIa, have been used, with inconsistent results...
November 28, 2016: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/27893618/early-resuscitation-with-lyophilized-plasma-provides-equal-neuroprotection-compared-with-fresh-frozen-plasma-in-a-large-animal-survival-model-of-traumatic-brain-injury-and-hemorrhagic-shock
#4
Ihab Halaweish, Ted Bambakidis, Vahagn C Nikolian, Patrick Georgoff, Peter Bruhn, Patryk Piascik, Lisa Buckley, Ashok Srinivasan, Baoling Liu, Yongqing Li, Hasan B Alam
BACKGROUND: Combined traumatic brain injury (TBI) and hemorrhagic shock (HS) is highly lethal. In previous models of combined TBI + HS, we showed that early resuscitation with fresh frozen plasma (FFP) improves neurologic outcomes. Delivering FFP, however, in austere environments is difficult. Lyophilized plasma (LP) is a logistically superior alternative to FFP, but data are limited regarding its efficacy for treatment of TBI. We conducted this study to determine the safety and long-term outcomes of early treatment with LP in a large animal model of TBI + HS...
December 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/27893348/cost-utility-and-budget-impact-of-methylene-blue-treated-plasma-compared-to-quarantine-plasma
#5
Joseph B Babigumira, Solomon J Lubinga, Emma Castro, Brian Custer
BACKGROUND: Methylene blue and visible light treatment and quarantine are two methods used to reduce adverse events, mostly infections, associated with the transfusion of fresh-frozen plasma. The objective of this study was to estimate and compare the budget impact and cost-utility of these two methods from a payer's perspective. MATERIALS AND METHODS: A budget impact and cost-utility model simulating the risks of hepatitis B virus, hepatitis C virus, cytomegalovirus, a West Nile virus-like infection, allergic reactions and febrile non-haemolytic transfusion reactions achieved using plasma treated with methylene blue and visible light (MBP) and quarantine plasma (QP) was constructed for Spain...
November 16, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27886001/strategy-for-the-hemocompatibility-testing-of-microparticles
#6
S Braune, S Basu, K Kratz, J Bäckemo Johansson, M Reinthaler, A Lendlein, F Jung
Polymer-based microparticles are applied as non-thrombogenic or thrombogenic materials in a wide variety of intra- or extra-corporeal medical devices. As demanded by the regulatory agencies, the hemocompatibility of these blood contacting biomaterials has to be evaluated in vitro to ensure that the particle systems appropriately fulfill the envisioned function without causing undesired events such as thrombosis or inflammation. Currently described in vitro assays for hemocompatibility testing of particles comprise tests with different single cell types (e...
November 23, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27851218/1583-mobile-application-training-platform-to-support-fresh-whole-blood-transfusion-for-civilian-emergency
#7
Elizabeth Mann-Salinas, Tricia Garcia, Oscar Pinzon, Ethan Miles, Andrew Cap
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27832801/gene-expression-comparison-reveals-distinct-basal-expression-of-hox-members-and-differential-tnf-induced-response-between-brain-and-spinal-cord-derived-microvascular-endothelial-cells
#8
Yves Molino, Françoise Jabès, Amandine Bonnet, Nicolas Gaudin, Anne Bernard, Philippe Benech, Michel Khrestchatisky
BACKGROUND: The heterogeneity of endothelial cell types underlies their remarkable ability to sub-specialize and provide specific requirements for a given vascular bed. Here, we compared rat microvascular endothelial cells (MECs) derived from the brain and spinal cord in both basal and inflammatory conditions. METHODS: We used whole rat genome microarrays to compare, at different time points, basal and TNF-α-induced gene expression of rat MECs from in vitro models of the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB)...
November 10, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27824651/infliximab-selectively-modulates-the-circulating-blood-monocyte-repertoire-in-crohn-s-disease
#9
Stephanie M Slevin, Michael Conall Dennedy, Eanna P Connaughton, Andreia Ribeiro, Rhodri Ceredig, Matthew D Griffin, Laurence J Egan
BACKGROUND: Infliximab (IFX), an anti-tumour necrosis factor alpha (TNFα) monoclonal antibody, provides clinical benefits in treating Crohn's disease (CD) but its mechanisms of action are not fully elucidated. This study investigated blood monocyte repertoires and the acute effects of IFX infusion on monocyte subset phenotype and function in IFX-treated patients with CD. METHODS: Monocytes and monocyte subsets were enumerated and phenotypically characterized by multicolor flow cytometry in freshly isolated blood from healthy controls (n = 21) and patients with CD treated with (IFX, n = 24) and without (non-IFX, n = 20) IFX...
December 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27816874/prevailing-vitamin-d-status-influences-mitochondrial-and-glycolytic-bioenergetics-in-peripheral-blood-mononuclear-cells-obtained-from-adults
#10
Emily K Calton, Kevin N Keane, Mario J Soares, Jordan Rowlands, Philip Newsholme
BACKGROUND: Circulating peripheral blood mononuclear cells (PBMCs) are exposed to metabolic and immunological stimuli that influence their functionality. We hypothesized that prevailing vitamin D status [25(OH)D] would modulate the bioenergetic profile of PBMCs derived from humans. MATERIALS AND METHODS: 38 participants (16 males, 22 females) ranging in body fat from 14-51% were studied. PBMCs were isolated from whole blood, counted and freshly seeded for bioenergetic analysis using the Seahorse XF(e)96 flux analyser...
December 2016: Redox Biology
https://www.readbyqxmd.com/read/27812303/the-influence-of-sample-freezing-at-80-%C3%A2-c-for-2-12-weeks-on-glycated-haemoglobin-hba1c-concentration-assayed-by-hplc-method-on-bio-rad-d-10-%C3%A2-auto-analyzer
#11
Katarzyna Bergmann, Grazyna Sypniewska
INTRODUCTION: The aim of the study was to evaluate the effect of a single freeze/thaw cycle on HbA1c concentrations measured by commercially available HPLC method. MATERIALS AND METHODS: Study included 128 whole blood samples collected from diabetic patients (N = 60) and healthy volunteers (N = 68). HbA1c concentrations were measured in fresh blood samples. Then samples were frozen at - 80 °C for up to 12 weeks. HbA1c was assayed by ion-exchange HPLC method on Bio-Rad D-10(®) analyzer...
October 15, 2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/27809835/anvillea-garcinii-extract-inhibits-the-oxidative-burst-of-primary-human-neutrophils
#12
Hanane Boukemara, Margarita Hurtado-Nedelec, Viviana Marzaioli, Dalila Bendjeddou, Jamel El Benna, Jean-Claude Marie
BACKGROUND: Anvillea garcinii Coss. & Durieu (Anv) plant is used as a traditional North African medicine against several diseases associated with inflammation. At inflammatory sites, reactive oxygen species (ROS) produced in excess by activated phagocyte NADPH oxidase (NOX2) can accentuate inflammatory responses. Thus, we investigated if Anv-water soluble polysaccharides could modulate primary human neutrophil oxidative burst in vitro. METHODS: Human neutrophils were isolated from fresh whole blood and O2(...
November 3, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27774338/quantification-of-cell-free-dna-in-red-blood-cell-units-in-different-whole-blood-processing-methods
#13
Andrew W Shih, Vinai C Bhagirath, Nancy M Heddle, Jason P Acker, Yang Liu, John W Eikelboom, Patricia C Liaw
Background. Whole blood donations in Canada are processed by either the red cell filtration (RCF) or whole blood filtration (WBF) methods, where leukoreduction is potentially delayed in WBF. Fresh WBF red blood cells (RBCs) have been associated with increased in-hospital mortality after transfusion. Cell-free DNA (cfDNA) is released by neutrophils prior to leukoreduction, degraded during RBC storage, and is associated with adverse patient outcomes. We explored cfDNA levels in RBCs prepared by RCF and WBF and different storage durations...
2016: Journal of Blood Transfusion
https://www.readbyqxmd.com/read/27754267/sy-16-1-dietary-approaches-to-prevent-and-control-elevated-blood-pressure
#14
Yongsoon Park
A healthy lifestyle is important in CVD prevention and treatment through effects on modifiable CVD risk factors, particularly blood pressure. Although the major drawback is the low level of adherence over time, appropriate lifestyle changes may safely and effectively prevent and treat hypertension. The recommended dietary approaches that have been shown to be capable of reducing blood pressure are: salt restriction, moderation of alcohol consumption, high consumption of vegetables and fruits and low-fat and other types of diet, and weight reduction...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27753035/digital-pcr-of-genomic-rearrangements-for-monitoring-circulating-tumour-dna
#15
Hongdo Do, Daniel Cameron, Ramyar Molania, Bibhusal Thapa, Gareth Rivalland, Paul L Mitchell, Carmel Murone, Thomas John, Anthony Papenfuss, Alexander Dobrovic
Identifying circulating tumour DNA (ctDNA) for monitoring of cancer therapy is dependent on the development of readily designed, sensitive cancer-specific DNA markers. Genomic rearrangements that are present in the vast majority of cancers provide such markers.Tumour DNA isolated from two fresh-frozen lung tumours underwent whole genome sequencing. Genomic rearrangements were detected using a new computational algorithm, GRIDSS. Four genomic rearrangements from each tumour were chosen for further study using rearrangement-specific primers...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27721707/extracellular-vesicles-from-red-blood-cell-products-induce-a-strong-pro-inflammatory-host-response-dependent-on-both-numbers-and-storage-duration
#16
Marleen Straat, Anita N Böing, Anita Tuip-De Boer, Rienk Nieuwland, Nicole P Juffermans
BACKGROUND: Transfusion of red blood cells (RBCs) is associated with adverse outcome, but the causative factor is unknown. Extracellular vesicles (EVs) have pro-inflammatory properties. We hypothesized that EVs released from both fresh and stored RBC products can induce a host inflammatory response in a dose-dependent manner. METHODS: Whole blood was incubated with supernatant from RBC units stored for different time periods, either containing (different numbers of) EVs or depleted from EVs...
July 2016: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/27718203/screening-vaccine-formulations-in-fresh-human-whole-blood
#17
Jalil Hakimi, Sepideh Aboutorabian, Frederick To, Salvador F Ausar, Nausheen Rahman, Roger H Brookes
Monitoring the immunological functionality of vaccine formulations is critical for vaccine development. While the traditional approach using established animal models has been relatively effective, the use of animals is costly and cumbersome, and animal models are not always reflective of a human response. The development of a human-based approach would be a major step forward in understanding how vaccine formulations might behave in humans. Here, we describe a platform methodology using fresh human whole blood (hWB) to monitor adjuvant-modulated, antigen-specific responses to vaccine formulations, which is amenable to analysis by standard immunoassays as well as a variety of other analytical techniques...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27658949/magnetic-resonance-imaging-appearance-and-mechanism-of-action-of-five-hemostatic-agents-used-in-neurosurgery
#18
Cord M Brundage, Rebecca A Packer, Matthew D Jones
OBJECTIVE: To describe the magnetic resonance (MR) image appearance of 5 hemostatic agents placed in the brain, and to review their clinical application. STUDY DESIGN: Descriptive ex vivo and in vivo study. ANIMALS: Canine cadavers (n=4), client-owned dogs (n=4). METHODS: Heads from 4 canine cadavers were used, each with 5 hemostatic agents placed in specific locations in the brain. Hemostatic agents were used in their native form in 2 cadaveric brains, and in 2 others the materials were saturated with fresh whole blood prior to placement to mimic application in a field of active hemorrhage...
September 23, 2016: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/27658911/effect-of-phyllostachys-parvifolia-leaf-extract-on-ionizing-radiation-induced-genetic-damage-a-preliminary-in%C3%A2-vitro-cytogenetic-study
#19
Mansi Patel, Priti Mehta, Sonal Bakshi, Shikha Tewari
The ionizing radiation is a known carcinogen as well as cancer therapeutic agent however, the side effect on normal tissue is a limiting factor and inadequate doses necessitates search for an ideal radioprotective agent. Bamboo species are rich source of antioxidants hence have therapeutic value in many free radical mediated diseases. This is the first report regarding in vitro protective effect of bamboo leaf extract against radiation induced genetic damage in human peripheral blood lymphocytes by cytokinesis blocked micronuclei (CBMN) assay...
July 2016: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/27648698/limited-resuscitation-with-fresh-or-stored-whole-blood-corrects-cardiovascular-and-metabolic-function-in-a-rat-model-of-polytrauma-and-hemorrhage
#20
Jacob Chen, Xiaowu Wu, Jeffrey Keesee, Bin Liu, Daniel N Darlington, Andrew P Cap
INTRODUCTION: We have recently shown that human whole blood stored at 4°C maintains hemostatic and platelet function. In this study, we compared restoration of hemodynamic, metabolic and hemostatic function after limited resuscitation with rat fresh whole blood, rat stored whole blood, or Lactated Ringers in traumatized rats. METHODS: Rat whole blood was stored for 10 days at 4°C for evaluation of hemostatic function. Polytrauma was performed on isoflurane-anesthetized Sprague-Dawley rats (350-450 g) by damage to the intestines, liver, right leg skeletal muscle and right femur fracture, followed by 40% hemorrhage...
September 19, 2016: Shock
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