keyword
MENU ▼
Read by QxMD icon Read
search

fresh whole blood

keyword
https://www.readbyqxmd.com/read/29770756/the-influence-of-juicing-on-the-appearance-of-blueberry-metabolites-2-h-after-consumption-a-metabolite-profiling-approach
#1
Swen Langer, Aileen Kennel, John K Lodge
The consumption of berries has been linked to decreased risk of degenerative disease. Berries are regularly processed into juices. It is largely unknown how the juicing process affects the bioavailability of metabolites. As metabolomics has shown to be a valuable nutritional tool to study global metabolite differences, the aim of this study was to investigate the effect of juicing on the relative appearance of blueberry metabolites in humans using metabolomics. Nine healthy subjects consumed 250 g of fresh blueberries either as the whole fruit or after juicing, and provided blood and urine samples before and 2 h after intake in a cross-over design...
June 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29760471/bioinspired-detoxification-of-blood-the-efficient-removal-of-anthrax-toxin-protective-antigen-using-an-extracorporeal-macroporous-adsorbent-device
#2
Ganesh Ingavle, Les Baillie, Nathan Davies, Nigel Beaton, Yishan Zheng, Sergey Mikhalovsky, Susan Sandeman
Whilst various remedial human monoclonal antibodies have been developed to treat the potentially life-threatening systemic complications associated with anthrax infection, an optimal and universally effective administration route has yet to be established. In the later stages of infection when antibody administration by injection is more likely to fail one possible route to improve outcome is via the use of an antibody-bound, adsorbent haemoperfusion device. We report here the development of an adsorbent macroporous polymer column containing immobilised B...
May 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29746819/continuous-thrombin-generation-in-whole-blood-new-applications-for-assessing-activators-and-inhibitors-of-coagulation
#3
Shannon M Prior, Kenneth G Mann, Kalev Freeman, Saulius Butenas
Hemostatic tests have been utilized to clarify the blood coagulation potential. The novel thrombin generation (TG) assay of this study provides explicit information and is the most physiologically-relevant hemostatic test ex vivo. We describe how this assay allows for TG under a number of relevant circumstances. First, whole blood (WB) from healthy individuals was analyzed ± 5 pM tissue factor (TF) and ± contact pathway inhibition. Without an exogenous initiator TG was decreased and delayed, but addition of 5 pM TF shortened the lag phase and increased peak thrombin...
May 7, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29740773/effect-of-calcium-lignosulfonate-supplementation-on-metabolic-profiles-of-confined-lambs
#4
Maria Luiza França Silva, Gleidson Giordano Pinto de Carvalho, Robério Rodrigues Silva, Tamires da Silva Magalhães, Pablo Teixeira Viana, Luana Marta de Almeida Rufino, Aracele Vieira Santos, José Augusto Gomes Azevedo, José Esler Freitas Júnior, Camila de Oliveira Nascimento, Carlos Emanuel Eiras
This study aimed to evaluate the effect of calcium lignosulfonate associated with whole cottonseed in high-concentrate diets for sheep. Eight Dorper crossbred sheep with an average live weight of 42.5 ± 1.70 kg were assigned to two 4 × 4 Latin squares. The following experimental diets were evaluated: control diet (without calcium lignosulfonate) and diets with inclusion of 50, 100, and 150 g of calcium lignosulfonate/kg fresh matter. Diets were composed of soybean meal, ground corn, and whole cottonseed...
May 9, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29728865/ultraviolet-photofunctionalization-of-nanostructured-titanium-surfaces-enhances-thrombogenicity-and-platelet-response
#5
Nagat Areid, Ilkka Kangasniemi, Eva Söderling, Timo O Närhi
The purpose of this study was to evaluate blood and platelet response to nanostructured TiO2 coatings and to investigate the effect of Ultraviolet (UV) light treatment on blood clotting ability, platelet activation and protein adhesion. Ti-6Al-4V titanium alloy plates (n = 138) were divided into three groups; a sol-gel derived MetAliveTM coating (MA); hydrothermal coating (HT); and a non-coated group (NC). Sixty nine titanium substrates were further treated with UV light for 1 h. The thrombogenicity of the titanium substrates was assessed using fresh human blood with a whole blood kinetic clotting time method...
May 4, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29728805/large-volume-crystalloid-fluid-is-associated-with-increased-hyaluronan-shedding-and-inflammation-in-a-canine-hemorrhagic-shock-model
#6
Lisa Smart, C J Boyd, M A Claus, E Bosio, G Hosgood, A Raisis
Shedding of the endothelial glycocalyx precedes leukocyte activation and adherence in acute inflammation. Rapid administration of crystalloid or colloid fluids for treating hemorrhagic shock may cause endothelial glycocalyx shedding, thereby increasing inflammation. This study aimed to compare the effect of different fluid treatments in a canine shock model on glycocalyx biomarker, hyaluronan, and inflammatory biomarkers. Greyhound dogs under general anesthesia subject to hemorrhage for 60 min were given 20 mL kg-1 of either fresh whole blood (FWB), hydroxyethyl starch (HES) 130/0...
May 4, 2018: Inflammation
https://www.readbyqxmd.com/read/29716634/successful-fertility-following-optimized-perfusion-and-cryopreservation-of-whole-ovary-and-allotransplantation-in-a-premature-ovarian-insufficiency-rat-model
#7
Yan Ding, Jia-Liang Shao, Jun-Wei Li, Ying Zhang, Kai-Hua Hong, Ke-Qin Hua, Xiang Wang
BACKGROUND: Fertility preservation by whole ovary cryopreservation and transplantation (WOCP&TP) with vascular anastomosis requires successful cryopreservation. In this study, we investigated the possibility of restoring ovarian function and natural fertility after WOCP&TP in a premature ovarian insufficiency (POI) rat model. The influence of cryopreservation on the offspring of rats following WOCP&TP was also explored. METHOD: Rats aged 8-10 weeks were used as donors and recipients for allotransplantation...
May 2, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29713955/successful-hemostatic-management-of-major-surgery-for-cervical-spondylotic-myelopathy-in-a-patient-with-severe-factor-xi-deficiency
#8
Yoshiyuki Ogawa, Kunio Yanagisawa, Yuri Uchiyama, Naoki Akashi, Tokue Mieda, Haku Iizuka, Madoka Inoue, Reiko Shizuka, Masami Murakami, Naomichi Matsumoto, Hiroshi Handa
Factor XI deficiency (FXID) is a rare bleeding disorder caused by mutations in the F11 gene. Spontaneous bleeding in patients with factor XI deficiency is rare, but major bleeding may occur after surgery or trauma. The basic method for hemostatic treatment is replacement of the missing factor using FXI concentrate or fresh frozen plasma (FFP). We report the case of a 72-year-old male with severe FXID who underwent a laminoplasty under sufficient, but minimal, FFP transfusion. Through detailed monitoring of activated partial thromboplastin time (APTT) and FXI activity at the perioperative period, we succeeded in hemostatic management of major surgery without significant blood loss and fluid overload...
April 30, 2018: International Journal of Hematology
https://www.readbyqxmd.com/read/29695033/action-of-the-metalloproteinases-in-gonadal-remodeling-during-sex-reversal-in-the-sequential-hermaphroditism-of-the-teleostei-fish-synbranchus-marmoratus-synbranchiformes-synbranchidae
#9
Talita Sarah Mazzoni, Fabiana Laura Lo Nostro, Fernanda Natália Antoneli, Irani Quagio-Grassiotto
Teleostei present great plasticity regarding sex change. During sex reversal, the whole gonad including the germinal epithelium undergoes significant changes, remodeling, and neoformation. However, there is no information on the changes that occur within the interstitial compartment. Considering the lack of information, especially on the role played by metalloproteinases (MMPs) in fish gonadal remodeling, the aim of this study was to evaluate the action of MMPs on gonads of sex reversed females of Synbranchus marmoratus , a fresh water protogynic diandric fish...
April 24, 2018: Cells
https://www.readbyqxmd.com/read/29694688/quantitative-susceptibility-mapping-and-23-na-imaging-based-in-vitro-characterization-of-blood-clotting-kinetics
#10
Till M Schneider, Armin M Nagel, Markus Zorn, Andreas Wetscherek, Martin Bendszus, Mark E Ladd, Sina Straub
Blood clotting is a fundamental biochemical process in post-hemorrhagic hemostasis. Although the varying appearance of coagulating blood in T1 - and T2 -weighted images is widely used to qualitatively determine bleeding age, the technique permits only a rough discrimination of coagulation stages, and it remains difficult to distinguish acute and chronic hemorrhagic stages because of low T1 - and T2 -weighted signal intensities in both instances. To investigate new biomedical parameters for magnetic resonance imaging-based characterization of blood clotting kinetics, sodium imaging and quantitative susceptibility mapping (QSM) were compared with conventional T1 - and T2 -weighted imaging, as well as with biochemical hemolysis parameters...
April 25, 2018: NMR in Biomedicine
https://www.readbyqxmd.com/read/29686986/blood-and-blood-components-from-similarities-to-differences
#11
REVIEW
Olivier Garraud, Jean-Daniel Tissot
Blood transfusion is made possible because, in most countries and organizations, altruistic individuals voluntarily, anonymously, and generously donate (without compensation) either whole blood or separated components that are then processed and distributed by professionals, prior to being allocated to recipients in need. Being part of modern medicine, blood transfusion uses so-called standard blood components when relative to cellular fractions and fresh plasma. However, as will be discussed in this paper, strictly speaking, such so-called labile blood components are not completely standard...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29649332/development-of-novel-est-ssr-markers-for-ploidy-identification-based-on-de-novo-transcriptome-assembly-for-misgurnus-anguillicaudatus
#12
Bing Feng, Soojin V Yi, Manman Zhang, Xiaoyun Zhou
The co-existence of several ploidy types in natural populations makes the cyprinid loach Misgurnus anguillicaudatus an exciting model system to study the genetic and phenotypic consequences of ploidy variations. A first step in such effort is to identify the specific ploidy of an individual. Currently popular methods of karyotyping via cytological preparation or flow cytometry require a large amount of tissue (such as blood) samples, which can be damaging or fatal to the fishes. Here, we developed novel microsatellite markers (SSR markers) from M...
2018: PloS One
https://www.readbyqxmd.com/read/29624852/cryopreserved-whole-blood-for-the-quantification-of-monocyte-t-cell-and-nk-cell-subsets-and-monocyte-receptor-expression-by-multi-color-flow-cytometry-a-methodological-study-based-on-participants-from-the-canadian-longitudinal-study-on-aging
#13
Chris P Verschoor, Vikas Kohli
Immunophenotyping by multi-color flow cytometry is arguably the best tool to identify and quantify distinct cell lineages from the peripheral blood and other biological fluids/tissues. Effective in both clinical and research settings, it can be used to estimate the frequency of a given cell type or measure its phenotypic or functional properties. Normally, immunophenotyping is performed in fresh or fractionated blood (i.e., PBMCs) the same day, or within 24 hours of collection; however, this may not be feasible for all study designs...
April 6, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29615382/severe-heinz-body-anemia-and-methemoglobinemia-in-a-kitten-with-chronic-diarrhea
#14
P Cavana, E Irato, B Miniscalco, P Gianella
A 2-month-old kitten was referred for depression and partial anorexia since 3 days and chronic diarrhea lasting for over 3 weeks. General physical examination showed pale and cyanotic mucous membranes. Blood sample was of brownish appearance. Venous blood gas analysis and complete blood count showed 16% methemoglobin level and severe regenerative anemia with Heinz bodies in about 40% of the erythrocytes, respectively. The kitten was transfused with fresh whole blood and treated with supportive care, antimicrobial and antioxidant agents...
April 2018: Schweizer Archiv Für Tierheilkunde
https://www.readbyqxmd.com/read/29614267/influence-of-delayed-sample-processing-on-blood-immune-cell-phenotypes-immune-cell-responses-and-serum-anti-influenza-vaccine-antibody-titres
#15
Vivian Castro-Herrera, Mark Lown, George Lewith, Elizabeth A Miles, Philip C Calder
Provision of blood from distant research partners to a central laboratory can result in delayed blood processing prior to assessment of immune parameters. It is important to evaluate the effect of such delays on immune parameters. This study investigated the effect of storage of blood at room temperature for up to 72 h prior to processing and analysis on a range of immune parameters. Blood was collected from 10 healthy participants and analysed immediately (day 0) or after storage at room temperature for 24, 48 or 72 h (days 1, 2 and 3)...
March 31, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29611226/susceptibility-of-a-fumigatus-specific-t-cell-assays-to-pre-analytic-blood-storage-and-pbmc-cryopreservation-greatly-depends-on-readout-platform-and-analytes
#16
Chris D Lauruschkat, Sebastian Wurster, Lukas Page, Maria Lazariotou, Mariola Dragan, Philipp Weis, Andrew J Ullmann, Hermann Einsele, Jürgen Löffler
Mould specific T-cells detectable by flow cytometry or ELISPOT were proposed as a novel biomarker in invasive aspergillosis. To define protocols facilitating sample shipment and longitudinal analysis, this study evaluated the susceptibility of different functional assays for A. fumigatus specific T-cell quantification and characterisation to pre-analytic delays. PBMCs from six healthy donors were analysed after immediate isolation, after 6 hours whole blood storage or after cryopreservation using three different common media...
April 3, 2018: Mycoses
https://www.readbyqxmd.com/read/29607859/challenges-in-the-management-of-bleeding-disorders-in-nigeria
#17
H C Okoye, K I Korubo, B Nwogoh, C C Efobi, N I Ugwu, A J Madu
Background: Bleeding disorders (BDs) are characterized by abnormal bleeding for which effective management requires a combination of skill, workforce, diagnostic facilities, and adequate therapeutic options. Objectives: The objectives of this study were to determine the capacity of Nigerian hematologists to handle BDs and to assess availability of required infrastructure, equipment, and treatment options. Materials and Methods: This descriptive study was conducted during the 2016 scientific conference of the Nigerian Society for Hemetology and Blood Transfusion...
April 2018: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29575062/restored-response-to-adp-downstream-of-purinergic-p2y-12-receptor-in-apheresis-platelets-after-pathogen-reducing-xenon-flash-treatment
#18
Hideki Abe, Takaaki Abe, Masayuki Shiba, Masahiro Satake
BACKGROUND: Our previous study revealed that pathogen-reducing filtered xenon flash-treated platelets (fXe-PLTs) showed sustained aggregation in response to adenosine diphosphate (ADP), but apheresis-collected PLTs (Aph-PLTs) showed reversible aggregation. STUDY DESIGN AND METHODS: Aph-PLTs, fXe-PLTs, and freshly prepared PLTs (PRP-PLTs) from whole blood were used to investigate the following responses to ADP: concentration response and effects of ADP receptor antagonists on aggregation, the cytosolic calcium (Ca2+ ) flux downstream of P2Y1 receptor signaling, and phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and signaling intermediate protein Akt downstream of the P2Y12 receptor...
March 25, 2018: Transfusion
https://www.readbyqxmd.com/read/29574278/sjtrec-quantification-using-sybr-quantitative-pcr-for-age-estimation-of-bloodstains-in-a-japanese-population
#19
Eisuke Yamanoi, Saori Uchiyama, Makoto Sakurada, Yasuhiro Ueno
Individual age is a phenotypic trait that provides useful information in forensic investigations. Levels of signal joint T-cell receptor rearrangement excision circle (sjTREC) in human peripheral blood are known to decline with increasing age. The advantages of sjTREC quantification are the simple procedures and highly accurate age estimation results. Whereas TaqMan quantification PCR (qPCR) is widely used for sjTREC quantification, SYBR qPCR assay is not routinely used for evaluating ethnic data. Therefore, we focused on the advantages of the SYBR qPCR assay, which is cheaper and simpler to set up than the TaqMan probe assay...
March 15, 2018: Legal Medicine
https://www.readbyqxmd.com/read/29553795/applicability-of-the-monocyte-activation-test-mat-in-the-quality-control-of-the-17dd-yellow-fever-vaccine
#20
Katherine Antunes de Mattos, Elaine Cristina Azevedo Navega, Vitor Fernandes Silva, Alessandra Santos Almeida, Cristiane Caldeira da Silva, Octavio Augusto França Presgrave, Daniel da Silva Guedes Junior, Isabella Fernandes Delgado
The need for alternatives to animal use in pyrogen testing has been driven by the Three Rs concept. This has resulted in the inclusion of the monocyte activation test (MAT) in the European Pharmacopoeia, 2010. However, some technical and regulatory obstacles must be overcome to ensure the effective implementation of the MAT by the industry, especially for the testing of biological products. The yellow fever (YF) vaccine (17DD-YFV) was chosen for evaluation in this study, in view of: a) the 2016-2018 outbreak of YF in Brazil; b) the increase in demand for 17DD-YFV doses; c) the complex production process with live attenuated virus; d) the presence of possible test interference factors, such as residual process components (e...
March 2018: Alternatives to Laboratory Animals: ATLA
keyword
keyword
120702
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"