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https://www.readbyqxmd.com/read/28217266/methodology-and-early-findings-of-the-fifth-survey-of-childhood-and-adolescence-surveillance-and-prevention-of-adult-noncommunicable-disease-the-caspian-v-study
#1
Mohammad Esmaeil Motlagh, Hasan Ziaodini, Mostafa Qorbani, Majzoubeh Taheri, Tahereh Aminaei, Azam Goodarzi, Asal Ataie-Jafari, Fatemeh Rezaei, Zeinab Ahadi, Gita Shafiee, Ali Shahsavari, Ramin Heshmat, Roya Kelishadi
BACKGROUND: This paper presents the methodology and early findings of the fifth survey of a school-based surveillance program in Iran. METHODS: This nationwide study was conducted in 2015 as the fifth survey of a surveillance program entitled "Childhood and Adolescence Surveillance and PreventIon of Adult Non- communicable disease" (CASPIAN-V) study. The protocol was mainly based on the World Health Organization-Global School student Health Survey. We studied 14400 students, aged 7-18 years, and their parents living in 30 provinces in Iran...
2017: International Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28210886/the-use-of-frozen-plasma-samples-in-thromboelastometry
#2
Christian Schoergenhofer, Nina Buchtele, Michael Schwameis, Johann Bartko, Bernd Jilma, Petra Jilma-Stohlawetz
Thromboelastometry is increasingly used in the clinical and scientific setting. The use of frozen plasma samples may be useful in overcoming certain limitations such as local and timely availability. Whole blood (WB) samples of 20 healthy volunteers were obtained, and plasma was generated. NATEM (n = 20), EXTEM (n = 20) and INTEM (n = 8) analyses were performed in WB, fresh plasma and frozen and thawed plasma. Dabigatran (500, 1000 ng/ml), rivaroxaban (100, 200 ng/ml) or alteplase (333 ng/ml) were added ex vivo to WB, and thromboelastometry was performed in WB and in frozen and thawed plasma samples...
February 16, 2017: Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/28184256/immunomethylomic-approach-to-explore-the-blood-neutrophil-lymphocyte-ratio-nlr-in-glioma-survival
#3
John K Wiencke, Devin C Koestler, Lucas A Salas, Joseph L Wiemels, Ritu P Roy, Helen M Hansen, Terri Rice, Lucie S McCoy, Paige M Bracci, Annette M Molinaro, Karl T Kelsey, Margaret R Wrensch, Brock C Christensen
BACKGROUND: Differentially methylated regions (DMRs) within DNA isolated from whole blood can be used to estimate the proportions of circulating leukocyte subtypes. We use the term "immunomethylomics" to describe the application of these immune lineage DMRs to studying leukocyte profiles. Here, we applied this approach to peripheral blood DNA from 72 glioma patients with molecularly defined brain tumors, representing common patient groups with defined characteristic survival times and risk factors...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28167274/characterization-of-human-leukocyte-huvec-adhesion-effect-of-cell-preparation-methods
#4
Franca Marino, Laura Schembri, Emanuela Rasini, Monica Pinoli, Angela Scanzano, Alessandra Luini, Terenzio Congiu, Marco Cosentino
OBJECTIVE: Sample manipulation to obtain isolated granulocytes represents a key, and often necessary, step in the in vitro studies. We investigated by the means of flow cytometry and microscopic techniques (both optical microscopy [OM] and scanning electron microscopy [SEM]), the granulocyte-endothelium adhesion and the role of sample manipulation. METHODS: By means of a co-culture method, we have analysed the adhesion of human leukocytes, originated from two different blood samples (fresh venous blood [FB] and buffy coat [BC]), to the human umbilical venous endothelial cell (HUVEC) monolayer...
February 3, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28149790/evaluation-of-vegf-gene-polymorphisms-and-proliferative-diabetic-retinopathy-in-mexican-population
#5
Roberto Gonzalez-Salinas, Maria C Garcia-Gutierrez, Gerardo Garcia-Aguirre, Virgilio Morales-Canton, Raul Velez-Montoya, Vidal R Soberon-Ventura, Victoria Gonzalez, Rodrigo Lechuga, Pablo Garcia-Solis, David G Garcia-Gutierrez, Marco Vinicio Garcia-Solis, Manuel Saenz de Viteri, Juan C Solis-S
AIM: To assess if the included vascular endothelial growth factor (VEGF) polymorphisms rs3025035, rs3025021 and rs2010963 are associated to proliferative retinopathy in a Mexican population with type 2 diabetes mellitus (T2DM). METHODS: A case-control study was conducted in adult individuals with T2DM associated to proliferative retinopathy or non-proliferative retinopathy from Oct. 2014 to Jun. 2015 from the Retina Department of the Asociation to Prevent Blindness in Mexico...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28043385/development-of-a-rapid-antibody-test-for-point-of-care-diagnosis-of-animal-african-trypanosomosis
#6
Alain Boulangé, Davita Pillay, Cyrille Chevtzoff, Nicolas Biteau, Vanessa Comé de Graça, Leonie Rempeters, Dimitrios Theodoridis, Théo Baltz
Trypanosoma congolense and T. vivax are the main causative agents of animal African trypanosomosis (AAT), a disease which hinders livestock production throughout sub-Saharan Africa and in some parts of South America. Although two trypanocidal drugs are currently available, the level of treatment is low due to the difficulty in diagnosing the disease in the field. The major clinical signs of AAT such as anaemia, weight loss, and infertility, are common to several other endemic livestock diseases. Current diagnostic methods, based on the visualization of the parasite in the blood, or on the detection of its DNA or the antibodies it triggers in the host, are not suitable for direct use in the field as they require specialized equipment and personnel...
January 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28009639/increased-expression-of-the-type-i-interferon-receptor-on-cd4-t-lymphocytes-in-hiv-1-infected-individuals
#7
M Scott Killian, Sue H Fujimura, Ramu G Sudhagoni
BACKGROUND: Type I interferons (IFN1; e.g. IFN-α and IFN-β) are potent cytokines that inhibit the replication of human immunodeficiency virus-1 (HIV-1) and other viruses. The antiviral and immunoregulatory activities of IFN1 are mediated through ligand/receptor interactions with the IFN1 receptor complex (IFNAR). Variation in the cell surface density of IFNAR could play a role in HIV-1 pathogenesis. METHODS: In this cross-sectional study of fresh whole blood, we used flow cytometry to evaluate the expression of IFNAR2 on lymphocyte subsets from HIV-1-infected (n=33) and -uninfected (n=22) individuals...
December 22, 2016: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28009388/acute-gastrointestinal-haemorrhage-on-board-a-cruise-ship-in-the-antarctic-peninsula
#8
Mathieu Carron, Peter Globokar, Bruno A Sicard
Antarctic tourism on board cruise ships has expanded since the 1990s, essentially in the Antarctic Peninsula. Due to remoteness, medical cases may evolve into life threatening conditions as emergency medical evacuations are challenging. We discuss the case of a young crew member who suddenly fainted with an epigastric pain and abundant rectal bleeding while on board a cruise ship heading to the Deception Island (62°57.6 South, 60°29.5 West), 44 h away from Ushuaia by sea. A medical evacuation was necessary to save the patient whose haemoglobin level rapidly decreased from 11 g/dL to 8...
2016: International Maritime Health
https://www.readbyqxmd.com/read/28003931/stability-of-thawed-apheresis-fresh-frozen-plasma-stored-for-up-to-120-hours-at-1%C3%A2-c-to-6%C3%A2-c
#9
William P Sheffield, Varsha Bhakta, Qi-Long Yi, Craig Jenkins
Regulations concerning the storage of transfusable plasma differ internationally. In Canada, plasma obtained from whole blood donations and frozen within 24 hours of phlebotomy (frozen plasma, FP) may be thawed and transfused within 120 hours of refrigerated storage. However, plasma frozen within 8 hours of phlebotomy following apheresis donation (FFPA) must be transfused within 24 hours of thawing and refrigeration. Our objectives were to measure coagulation factors (F) V, VII, and VIII, fibrinogen activities, and the prothrombin time (PT) in thawed refrigerated FFPA at 0, 24, and 120 hours of storage and to compare these values to those in thawed refrigerated FP...
2016: Journal of Blood Transfusion
https://www.readbyqxmd.com/read/28002053/in-vitro-thrombolytic-efficacy-of-echogenic-liposomes-loaded-with-tissue-plasminogen-activator-and-octafluoropropane-gas
#10
Himanshu Shekhar, Kenneth B Bader, Shenwen Huang, Tao Peng, Shaoling Huang, David D McPherson, Christy K Holland
Echogenic liposomes loaded with the thrombolytic recombinant tissue-type plasminogen activator (rt-PA) are under development for the treatment of ischemic stroke. These agents are designed to co-encapsulate cavitation nuclei to promote bubble activity in response to ultrasound exposure, and to enable localized delivery of thrombolytic. Stable cavitation improves the efficacy of the thrombolytic through enhanced fluid mixing. Echogenic liposomes that encapsulate air-filled microbubbles nucleate scant stable cavitation activity in response to 120 kHz intermittent ultrasound exposure, and have demonstrated thrombolytic efficacy equivalent to rt-PA alone...
December 21, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/27992495/options-to-expand-hiv-viral-load-testing-in-south-africa-evaluation-of-the-genexpert%C3%A2-hiv-1-viral-load-assay
#11
Natasha Gous, Lesley Scott, Leigh Berrie, Wendy Stevens
BACKGROUND: Expansion of HIV viral load (VL) testing services are required to meet increased targets for monitoring patients on antiretroviral treatment. South Africa currently tests >4million VLs per annum in 16 highly centralised, automated high-throughput laboratories. The Xpert HIV-1 VL assay (Cepheid) was evaluated against in-country predicates, the Roche Cobas Taqmanv2 and Abbott HIV-1RT, to investigate options for expanding VL testing using GeneXpert's random access, polyvalent capabilities and already established footprint in South Africa with the Xpert MTB/RIF assay (207 sites)...
2016: PloS One
https://www.readbyqxmd.com/read/27938877/coagulopathy-and-transfusion-requirements-in-war-related-penetrating-traumatic-brain-injury-a-single-centre-study-in-a-french-role-3-medical-treatment-facility-in-afghanistan
#12
J Bordes, C Joubert, P Esnault, A Montcriol, C Nguyen, E Meaudre, R Dulou, A Dagain
INTRODUCTION: Traumatic brain injury associated coagulopathy is frequent, either in isolated traumatic brain injury in civilian practice and in combat traumatic brain injury. In war zone, it is a matter of concern because head and neck are the second most frequent site of wartime casualty burden. Data focusing on transfusion requirements in patients with war related TBI coagulopathy are limited. MATERIALS AND METHODS: A descriptive analysis was conducted of 77 penetrating traumatic brain injuries referred to a French role 3 medical treatment facility in Kabul, Afghanistan, deployed on the Kabul International Airport (KaIA), over a 30 months period...
November 21, 2016: Injury
https://www.readbyqxmd.com/read/27919520/the-nanjing-glycated-hemoglobin-standardization-program-2012-to-2015
#13
Kui Zhang, Yan Wang, Hongxia Wei, Zhiye Xu
PURPOSE: This study aims to investigate glycosylated hemoglobin (HbA1c), between-laboratory imprecision (%CV), and pass rates from 2012 to 2015 in Nanjing to provide evidence for improving the HbA1c measurement. METHODS: This was a retrospective, descriptive analysis of HbA1c levels obtained from participating hospitals in Nanjing from 2012 to 2015. The HbA1c levels of fresh whole blood samples taken from healthy and diabetic adults were determined within 24 hours...
January 2017: Clinical Therapeutics
https://www.readbyqxmd.com/read/27916603/oxidative-stress-in-sepsis-possible-production-of-free-radicals-through-an-erythrocyte-mediated-positive-feedback-mechanism
#14
Yanaihara Pinchemel Amorim de Oliveira, Lain Carlos Pontes-de-Carvalho, Ricardo David Couto, Alberto Augusto Noronha-Dutra
BACKGROUND: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis. METHODS: Reactive oxygen specie production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals...
January 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27895055/role-of-agents-for-reversing-the-effects-of-target-specific-oral-anticoagulants
#15
REVIEW
Tanya R Riley, Mary L Gauthier-Lewis, Chelsea K Sanchez, Janine S Douglas
PURPOSE: The available clinical data on target-specific oral anticoagulant (TSOAC) reversal agents that are currently in development or have been approved by the Food and Drug Administration (FDA) are reviewed. SUMMARY: The development of TSOACs such as dabigatran, rivaroxaban, edoxaban, and apixaban has presented benefits and new challenges. One of the main challenges associated with the use of TSOACs is the lack of suitable agent-specific reversal agents. Several treatment options for the management of life-threatening bleeding events associated with TSOAC use, such as fresh frozen plasma, prothrombin complex concentrates, and recombinant coagulation factor VIIa, have been used, with inconsistent results...
January 15, 2017: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/27893618/early-resuscitation-with-lyophilized-plasma-provides-equal-neuroprotection-compared-with-fresh-frozen-plasma-in-a-large-animal-survival-model-of-traumatic-brain-injury-and-hemorrhagic-shock
#16
Ihab Halaweish, Ted Bambakidis, Vahagn C Nikolian, Patrick Georgoff, Peter Bruhn, Patryk Piascik, Lisa Buckley, Ashok Srinivasan, Baoling Liu, Yongqing Li, Hasan B Alam
BACKGROUND: Combined traumatic brain injury (TBI) and hemorrhagic shock (HS) is highly lethal. In previous models of combined TBI + HS, we showed that early resuscitation with fresh frozen plasma (FFP) improves neurologic outcomes. Delivering FFP, however, in austere environments is difficult. Lyophilized plasma (LP) is a logistically superior alternative to FFP, but data are limited regarding its efficacy for treatment of TBI. We conducted this study to determine the safety and long-term outcomes of early treatment with LP in a large animal model of TBI + HS...
December 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/27893348/cost-utility-and-budget-impact-of-methylene-blue-treated-plasma-compared-to-quarantine-plasma
#17
Joseph B Babigumira, Solomon J Lubinga, Emma Castro, Brian Custer
BACKGROUND: Methylene blue and visible light treatment and quarantine are two methods used to reduce adverse events, mostly infections, associated with the transfusion of fresh-frozen plasma. The objective of this study was to estimate and compare the budget impact and cost-utility of these two methods from a payer's perspective. MATERIALS AND METHODS: A budget impact and cost-utility model simulating the risks of hepatitis B virus, hepatitis C virus, cytomegalovirus, a West Nile virus-like infection, allergic reactions and febrile non-haemolytic transfusion reactions achieved using plasma treated with methylene blue and visible light (MBP) and quarantine plasma (QP) was constructed for Spain...
November 16, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27886001/strategy-for-the-hemocompatibility-testing-of-microparticles
#18
S Braune, S Basu, K Kratz, J Bäckemo Johansson, M Reinthaler, A Lendlein, F Jung
Polymer-based microparticles are applied as non-thrombogenic or thrombogenic materials in a wide variety of intra- or extra-corporeal medical devices. As demanded by the regulatory agencies, the hemocompatibility of these blood contacting biomaterials has to be evaluated in vitro to ensure that the particle systems appropriately fulfill the envisioned function without causing undesired events such as thrombosis or inflammation. Currently described in vitro assays for hemocompatibility testing of particles comprise tests with different single cell types (e...
2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27851218/1583-mobile-application-training-platform-to-support-fresh-whole-blood-transfusion-for-civilian-emergency
#19
Elizabeth Mann-Salinas, Tricia Garcia, Oscar Pinzon, Ethan Miles, Andrew Cap
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27832801/gene-expression-comparison-reveals-distinct-basal-expression-of-hox-members-and-differential-tnf-induced-response-between-brain-and-spinal-cord-derived-microvascular-endothelial-cells
#20
Yves Molino, Françoise Jabès, Amandine Bonnet, Nicolas Gaudin, Anne Bernard, Philippe Benech, Michel Khrestchatisky
BACKGROUND: The heterogeneity of endothelial cell types underlies their remarkable ability to sub-specialize and provide specific requirements for a given vascular bed. Here, we compared rat microvascular endothelial cells (MECs) derived from the brain and spinal cord in both basal and inflammatory conditions. METHODS: We used whole rat genome microarrays to compare, at different time points, basal and TNF-α-induced gene expression of rat MECs from in vitro models of the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB)...
November 10, 2016: Journal of Neuroinflammation
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