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https://www.readbyqxmd.com/read/29458535/isolation-characterization-and-genomic-analysis-of-a-novel-lytic-bacteriophage-vb_ssos-isf002-infecting-shigella-sonnei-and-shigella-flexneri
#1
Khashayar Shahin, Majid Bouzari, Ran Wang
PURPOSE: Shigellosis is one of the most important food-borne and water-borne diseases worldwide. Although antibiotics are considered as efficient agents for shigellosis treatment, improper use of these has led to the emergence of antibiotic-resistant Shigella spp. Therefore, finding a new strategy as alternative treatment seems necessary. METHODOLOGY: Different samples from a wastewater treatment plant were used to isolate Shigella spp. specific phages. Physiological properties were determined, and genomic analysis was also carried out...
January 30, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29397500/its2-sequence-structure-phylogeny-reveals-diverse-endophytic-pseudocercospora-fungi-on-poplars
#2
Dong-Hui Yan, Qian Gao, Xiaoming Sun, Xiaoyu Song, Hongchang Li
For matching the new fungal nomenclature to abolish pleomorphic names for a fungus, a genus Pseudocercospora s. str. was suggested to host holomorphic Pseudocercosproa fungi. But the Pseudocercosproa fungi need extra phylogenetic loci to clarify their taxonomy and diversity for their existing and coming species. Internal transcribed spacer 2 (ITS2) secondary structures have been promising in charactering species phylogeny in plants, animals and fungi. In present study, a conserved model of ITS2 secondary structures was confirmed on fungi in Pseudocercospora s...
February 3, 2018: Genetica
https://www.readbyqxmd.com/read/29395391/a-crybb2-mutation-in-a-taiwanese-family-with-autosomal-dominant-cataract
#3
Yung-Hao Ching, Jih-I Yeh, Wen-Lang Fan, Ko-Chen Chen, Man-Chieh Yeh, Peng Yeong Woon, Yuan-Chieh Lee
BACKGROUND/PURPOSE: To identify the underlying genetic cause of a Taiwanese family with autosomal dominant cerulean cataract. METHODS: A three-generation cerulean cataract family with 13 affected and 13 normal was identified. Whole exome sequencing, whole genome single nucleotide polymorphism genotyping and haplotype analysis, and fine mapping using polymorphic short tandem repeat markers were used to identify the causative gene mutation. RESULTS: Whole genome single nucleotide polymorphism genotyping and haplotype analysis mapped the candidate disease loci to chromosome 18 and chromosome 22...
January 27, 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/29371793/arthothelium-punctatum-arthoniaceae-arthoniales-a-new-lichen-species-from-south-korea
#4
Jung Shin Park, Sook-Young Park, Chan-Ho Park, Seol-Hwa Jang, Jae-Seoun Hur
A total of 121 species of lichens belonging to the genus Arthothelium have been described to date, most of which have been found in tropical regions. Here, we describe the discovery of a novel Arthothelium species for the first time in South Korea. Until now, Arthothelium ruanum was the only Arthothelium species reported in South Korea. Among the 113 specimens collected in this study, we identified A. ruanum and a putative new species, Arthothelium punctatum (J. S. Park & J.-S. Hur, sp. nov.). The diagnostic characters of A...
December 2017: Mycobiology
https://www.readbyqxmd.com/read/29364528/phylogenetic-expression-and-functional-characterizations-of-the-maize-nlp-transcription-factor-family-reveal-a-role-in-nitrate-assimilation-and-signaling
#5
Zhangkui Wang, Lei Zhang, Ci Sun, Riliang Gu, Guohua Mi, Lixing Yuan
Although nitrate represents an important nitrogen (N) source for maize, a major crop of dryland areas, the molecular mechanisms of nitrate uptake and assimilation remain poorly understood. Here, we identified nine maize NIN-like protein (ZmNLP) genes and analyzed the function of one member, ZmNLP3.1, in nitrate nutrition and signaling. The NLP family genes were clustered into three clades in a phylogenic tree. Comparative genomic analysis showed that most ZmNLP genes had collinear relationships to the corresponding NLPs in rice, and that the expansion of the ZmNLP family resulted from segmental duplications in the maize genome...
January 24, 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/29238330/an-assessment-of-different-genomic-approaches-for-inferring-phylogeny-of-listeria-monocytogenes
#6
Clémentine Henri, Pimlapas Leekitcharoenphon, Heather A Carleton, Nicolas Radomski, Rolf S Kaas, Jean-François Mariet, Arnaud Felten, Frank M Aarestrup, Peter Gerner Smidt, Sophie Roussel, Laurent Guillier, Michel-Yves Mistou, René S Hendriksen
Background/objectives: Whole genome sequencing (WGS) has proven to be a powerful subtyping tool for foodborne pathogenic bacteria like L. monocytogenes. The interests of genome-scale analysis for national surveillance, outbreak detection or source tracking has been largely documented. The genomic data however can be exploited with many different bioinformatics methods like single nucleotide polymorphism (SNP), core-genome multi locus sequence typing (cgMLST), whole-genome multi locus sequence typing (wgMLST) or multi locus predicted protein sequence typing (MLPPST) on either core-genome (cgMLPPST) or pan-genome (wgMLPPST)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29156776/uchl1-expression-and-localization-on-testicular-development-and-spermatogenesis-of-chinese-giant-salamanders
#7
Yuanxian Wang, Liqing Wang, Huihui Gao, Yao Gao, Changming Yang, Hong Ji, Wuzi Dong
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1), which is extensively expressed in vertebrates, is a deubiquitinating enzymes that inhibits the degradation of proteins by reversing ubiquitination modification. Herein, a 1087-bp sequence encoding UCHL1 was identified from the Chinese giant salamander (CGS; Andrias davidianus). The coding sequences (CDS) of UCHL1 encoded a putative poly peptide of 222 amino acids. The CGS UCHL1 isoforms were more related to their human and mouse counterparts. The phylogenic tree of vertebrate UCHL1 indicated that CGS UCHL1 has the closest relationship with human UCHL1 (up to 73...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29146256/isolation-purification-structural-elucidation-and-antimicrobial-activities-of-kocumarin-a-novel-antibiotic-isolated-from-actinobacterium-kocuria-marina-cmg-s2-associated-with-the-brown-seaweed-pelvetia-canaliculata
#8
Bushra Uzair, Farid Menaa, Barkat Ali Khan, Faryal Vali Mohammad, Viqar Uddin Ahmad, Ryad Djeribi, Bouzid Menaa
AIMS: Screening of seaweed-associated bacteria capable of producing antimicrobials. METHODS AND RESULTS: Fifteen microbial strains, associated to the brown seaweed Pelvetia canaliculata (Linnaeus) attached to the rocks of Sonmiani Beach (Karachi, Pakistan), were screened. Crude extract filtrates of CMG S2 strain grew on Zobell marine agar (ZMA) had the most remarkable antimicrobial activity. Based on its phenotypic aspects (e.g. Gram-positive, microccoid form), biochemical characteristics (e...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29136663/phylogenic-inference-using-alignment-free-methods-for-applications-in-microbial-community-surveys-using-16s-rrna-gene
#9
Yifei Zhang, Alexander V Alekseyenko
The diversity of microbiota is best explored by understanding the phylogenetic structure of the microbial communities. Traditionally, sequence alignment has been used for phylogenetic inference. However, alignment-based approaches come with significant challenges and limitations when massive amounts of data are analyzed. In the recent decade, alignment-free approaches have enabled genome-scale phylogenetic inference. Here we evaluate three alignment-free methods: ACS, CVTree, and Kr for phylogenetic inference with 16s rRNA gene data...
2017: PloS One
https://www.readbyqxmd.com/read/29126839/the-epidemic-typhus-and-trench-fever-are-risk-for-public-health-due-to-increased-migration-in-southeast-of-turkey
#10
Nilgün Ulutasdemir, Fadime Eroglu, Mustafa Tanrıverdi, Eda Icbay Dagli, Ismail Soner Koltas
Pediculus humanus capitis is a small ectoparasitic insect that has lived and feds on human beings for thousands of years. Molecular techniques have been used for Pediculus species identification and evolutionary, phylogenic, and ecological studies. A total of 23 adults of P. h. capitis were collected in Gaziantep, located in southeast Turkey, and DNA was isolated from all P. h. capitis using DNA extraction kit. All DNA samples were screened for investigate of Ricettsia prowazekii, Bartonella quintana and Borrelia recurrentis with real-time polymerase chain reaction...
November 7, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29102126/development-of-cdv-specific-monoclonal-antibodies-for-differentiation-of-variable-epitopes-of-nucleocapsid-protein
#11
Zhenwei Bi, Yongshan Wang, Qunxing Pan, Xingxia Xia, Libo Xu
The highly contagious canine distemper viruses (CDVs) are still a major threat to a wide range of natural susceptible hosts. The nucleocapsid (N) protein plays various roles in the virus-induced immune response. But precise mapping of epitopes and antigenic variations in N protein of CDV are still scant. In this study, two monoclonal antibodies (MAbs), designated as F8N and G3N, against the N protein of CDV were generated and characterized. The epitopes recognized by the two MAbs were mapped by truncated N protein fragments expressed in E...
November 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29081439/isolation-of-antibiotic-producing-pseudomonas-species-with-low-temperature-cultivation-of-temperate-soil
#12
Shuhei Mitsutomi, Kazuhisa Sekimizu, Chikara Kaito
We performed low-temperature cultivation of soil samples in Tokyo, Japan, and isolated 30 bacterial strains that formed colonies at 4°C. All the culture supernatants of these bacteria exhibited antibacterial activity against Escherichia coli. The 16S rDNA sequences of 29 strains showed similarity to that of the Pseudomonas genus, whereas the 16S rDNA sequence of one strain showed similarity to that of the Janthinobacterium genus. We classified the 29 strains into 10 groups according to the 16S rDNA sequence similarities, and performed two phylogenetic analyses using the 16S rDNA and rpoD gene sequences...
October 29, 2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28966259/phylogenic-analysis-and-evaluation-of-ephedra-plants-and-aconites-for-medicinal-use
#13
Nobuko Kakiuchi
Although Kampo medicine is now fully integrated into the modern Japanese healthcare system, most Kampo formulations depend on imported crude drugs from limited foreign areas. To prepare for possible shortages of crude drugs in the future, a wider scope for the supply of medicinal plants is necessary. We conducted field research and collaborated with international laboratories for phylogenic analysis and evaluation of medicinal plant resources. Our research on ephedra plants from a wide region of Eurasia has, for example, confirmed their phylogenic structure: based on DNA sequencing analysis of nuclear ribosomal internal transcribed spacer region 1 (ITS1) as well as the chloroplast intergenic spacer between trnL and trnF (trnL-F), the 8 major Chinese species and related plants grown on the continent could be divided into 3 groups...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28961520/food-poisoning-outbreak-in-tokyo-japan-caused-by-staphylococcus-argenteus
#14
Yasunori Suzuki, Hiroaki Kubota, Hisaya K Ono, Makiko Kobayashi, Konomi Murauchi, Rei Kato, Akihiko Hirai, Kenji Sadamasu
Staphylococcus argenteus is a novel species subdivided from Staphylococcus aureus. Whether this species can cause food poisoning outbreaks is unknown. This study aimed to investigate the enterotoxigenic activities of two food poisoning isolates suspected to be S. argenteus (Tokyo13064 and Tokyo13069). The results for phylogenic trees, constructed via whole genome sequencing, demonstrated that both isolates were more similar to a type strain of S. argenteus (MSHR1132) than any S. aureus strain. Moreover, the representative characteristics of S...
December 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28840340/trait-shifts-associated-with-the-subshrub-life-history-strategy-in-a-tropical-savanna
#15
A B Giroldo, A Scariot, W A Hoffmann
Over the past 10 million years, tropical savanna environments have selected for small growth forms within woody plant lineages. The result has been the evolution of subshrubs (geoxyles), presumably as an adaptation to frequent fire. To evaluate the traits associated with the shift from tree to subshrub growth forms, we compared seed biomass, germination, survival, resprouting, biomass allocation, and photosynthesis between congeneric trees and subshrubs, and quantified phylogenetic conservatism. Despite large differences in adult morphology between trees and subshrub species, the differences are modest in seedlings, and most of the variation in traits was explained by genus, indicating considerable phylogenic conservatism...
October 2017: Oecologia
https://www.readbyqxmd.com/read/28780378/homblocks-a-multiple-alignment-construction-pipeline-for-organelle-phylogenomics-based-on-locally-collinear-block-searching
#16
Guiqi Bi, Yunxiang Mao, Qikun Xing, Min Cao
Organelle phylogenomic analysis requires precisely constructed multi-gene alignment matrices concatenated by pre-aligned single gene datasets. For non-bioinformaticians, it can take days to weeks to manually create high-quality multi-gene alignments comprising tens or hundreds of homologous genes. Here, we describe a new and highly efficient pipeline, HomBlocks, which uses a homologous block searching method to construct multi-gene alignments. This approach can automatically recognize locally collinear blocks among organelle genomes and excavate phylogenetically informative regions to construct multi-gene alignment in a few hours...
August 2, 2017: Genomics
https://www.readbyqxmd.com/read/28642977/molecular-characteristics-and-prevalence-of-small-ruminant-lentiviruses-in-goats-in-japan
#17
Saki Kokawa, Mami Oba, Teppei Hirata, Shiro Tamaki, Miki Omura, Shinobu Tsuchiaka, Makoto Nagai, Tsutomu Omatsu, Tetsuya Mizutani
Small ruminant lentiviruses (SRLVs), which comprise caprine arthritis-encephalitis virus (CAEV) and maedi-visna virus (MVV), are prevalent in goats and sheep worldwide, including in Japan. However, little is known about the molecular characteristics of goat lentiviruses in Japan. In this study, a molecular and phylogenetic analysis of the long gag region was performed. The phylogenic tree demonstrated that all samples belonged to SRLV subtype B1. Two clusters were identified, with one cluster distinct from previously reported strains of subtype B1...
June 22, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28491250/prevalence-of-hepatitis-c-virus-genotype-3a-in-patients-with-hodgkin-and-non-hodgkin-lymphoma
#18
Hashem Radmehr, Manoochehr Makvandi, Alireza Samarbafzadeh, Ali Teimoori, Niloofar Neisi, Mojtaba Rasti, Sara Abasifar, Hasan Soltani, Samaneh Abbasi, Hadis Kiani, Hamide Mehravaran, Azarakhsh Azaran, Toran Shahani
BACKGROUND AND OBJECTIVES: Hepatitis C virus (HCV) is a major public health problem worldwide. Replication and persistence of HCV genome have been described in the liver tissue as well as B cells lymphocyte. Several investigations have reported that long-term persistence of HCV in B cells may result in Hodgkin and Non-Hodgkin lymphoma. This study was aimed to determine frequency of HCV RNA in histological tissues obtained from patients suffered from Hodgkin and Non-Hodgkin lymphoma. MATERIALS AND METHODS: 52 formalin-fixed paraffin-embedded tissue blocks including 23 (44...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28466379/bayesian-and-phylogenic-approaches-for-studying-relationships-among-table-olive-cultivars
#19
Rayda Ben Ayed, Karim Ennouri, Fathi Ben Amar, Fabienne Moreau, Mohamed Ali Triki, Ahmed Rebai
To enhance table olive tree authentication, relationship, and productivity, we consider the analysis of 18 worldwide table olive cultivars (Olea europaea L.) based on morphological, biological, and physicochemical markers analyzed by bioinformatic and biostatistic tools. Accordingly, we assess the relationships between the studied varieties, on the one hand, and the potential productivity-quantitative parameter links on the other hand. The bioinformatic analysis based on the graphical representation of the matrix of Euclidean distances, the principal components analysis, unweighted pair group method with arithmetic mean, and principal coordinate analysis (PCoA) revealed three major clusters which were not correlated with the geographic origin...
August 2017: Biochemical Genetics
https://www.readbyqxmd.com/read/28440467/structural-and-evolutionary-divergence-of-aquaporins-in-parasites-review
#20
Zi-Xin Ni, Jian-Min Cui, Nian-Zhang Zhang, Bao-Quan Fu
Aquaporins are ubiquitous proteins that belong to the major intrinsic protein family. Previous studies have indicated that aquaporins are involved in multiple physiological processes in parasites, such as nutrient absorption and end product efflux, and thus, would be promising pharmacological agents in the fight against parasite infection. In the present paper, the authors analyzed the evolutionary relationship of parasitic aquaporins by re‑constructing of a phylogenic tree using neighbor‑joining and maximum likelihood methods...
June 2017: Molecular Medicine Reports
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