Marshall Jaroch, Guangxin Sun, Ho-Ching Tiffany Tsui, Colbie Reed, Jingjing Sun, Marko Jörg, Malcolm E Winkler, Kelly C Rice, Agnieszka Dziergowska, Troy A Stich, Peter C Dedon, Patricia C Dos Santos, Valérie de Crécy-Lagard
The wobble bases of tRNAs that decode split codons are often heavily modified. In bacteria, tRNAGlu, Gln, Asp contains a variety of xnm5 s2 U derivatives. The synthesis pathway for these modifications is complex and fully elucidated only in a handful of organisms, including the Gram-negative Escherichia coli K12 model. Despite the ubiquitous presence of mnm5 s2 U modification, genomic analysis shows the absence of mnmC orthologous genes, suggesting the occurrence of alternate biosynthetic schemes for the conversion of cmnm5 s2 U to mnm5 s2 U...
March 29, 2024: Journal of Bacteriology