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https://www.readbyqxmd.com/read/29142410/methanolic-extract-of-costus-pictus-d-don-induces-cytotoxicity-in-liver-hepatocellular-carcinoma-cells-mediated-by-histone-deacetylase-inhibition
#1
P V Neethu, K Suthindhiran, M A Jayasri
Background: Leaves of Costus pictus D. Don, (insulin plant) are used as dietary supplement for the treatment of diabetes. Objective: The antidiabetic activity of this plant is well documented, but its activity on different cell types and mechanism remains unknown. Thus, the present study evaluates the cytotoxicity of C. pictus methanolic extract (CPME) against various cancer and normal cells. Materials and Methods: Dried leaves of C. pictus were extracted using methanol and were subjected to histone deacetylase (HDAC) inhibition and toxicity studies...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29137858/human-papillomavirus-e6-and-e7-oncoproteins-affect-the-cell-microenvironment-by-classical-secretion-and-extracellular-vesicles-delivery-of-inflammatory-mediators
#2
Marco Iuliano, Giorgio Mangino, Maria Vincenza Chiantore, Maria Simona Zangrillo, Rosita Accardi, Massimo Tommasino, Gianna Fiorucci, Giovanna Romeo
The connection between chronic inflammation and risk of cancer has been supported by several studies. The development of cancer might be a process driven by the presence of a specific combination of inflammatory mediators, including cytokines, chemokines and enzymes, in the tumor microenvironment. Virus-induced tumors, like HPV-induced Squamous Cell Carcinomas, represent a paradigmatic example of the interplay between inflammation, as integral part of the innate antiviral response, and malignant transformation...
November 11, 2017: Cytokine
https://www.readbyqxmd.com/read/29137231/an-oral-keratinocyte-life-cycle-model-identifies-novel-host-genome-regulation-by-human-papillomavirus-16-relevant-to-hpv-positive-head-and-neck-cancer
#3
Michael R Evans, Claire D James, Oonagh Loughran, Tara J Nulton, Xu Wang, Molly L Bristol, Brad Windle, Iain M Morgan
Many aspects of the HPV life cycle have been characterized in cervical cell lines (W12, CIN612) and in HPV immortalized primary foreskin keratinocytes. There is now an epidemic of HPV positive oropharyngeal cancers (HPV16 is responsible for 80-90% of these); therefore increased understanding of the HPV16 life cycle in oral keratinocytes is a priority. To date there have been limited reports characterizing the HPV16 life cycle in oral keratinocytes. Using TERT immortalized "normal" oral keratinocytes (NOKs) we generated clonal cell lines maintaining the HPV16 genome as an episome, NOKs+HPV16...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29118436/combining-membrane-proteomics-and-computational-three-way-pathway-analysis-revealed-signalling-pathways-preferentially-regulated-in-human-ipscs-and-human-escs
#4
Wei-Sheng Tien, Pei-Mien Chen, Ching-Yu Chuang, Shook-Mun Lui, Hung-Chih Kuo, Yu-Ju Chen, Kun-Pin Wu
Owing to the clinical potential of human induced pluripotent stem cells (hiPSCs) in regenerative medicine, a thorough examination of the similarities and differences between hiPSCs and human embryonic stem cells (hESCs) has become indispensable. Moreover, as the important roles of membrane proteins in biological signalling, functional analyses of membrane proteome are therefore promising. In this study, a pathway analysis by the bioinformatics tool GSEA was first performed to identify significant pathways associated with the three comparative membrane proteomics experiments: hiPSCs versus precursor human foreskin fibroblasts (HFF), hESCs versus precursor HFF, and hiPSCs versus hESCs...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29117186/human-papillomavirus-type-16-e6-and-nfx1-123-mislocalize-immune-signaling-proteins-and-downregulate-immune-gene-expression-in-keratinocytes
#5
Justine Levan, Portia A Vliet-Gregg, Kristin L Robinson, Rachel A Katzenellenbogen
Human papillomavirus (HPV) is the most prevalent sexually transmitted infection, affecting an estimated 11% of the world's population. The high-risk HPV types (HR HPV) account for approximately 5% of the global burden of cancer and thus cause high morbidity and mortality. Although it is known that persistent infection with HR HPV is the greatest risk factor for developing HPV-associated cancer, and that the HPV early proteins E6 and E7 dysregulate immune detection by its host cells, the mechanisms of immune evasion by HR HPV are not well understood...
2017: PloS One
https://www.readbyqxmd.com/read/29114247/elusive-role-of-the-cd94-nkg2c-nk-cell-receptor-in-the-response-to-cytomegalovirus-novel-experimental-observations-in-a-reporter-cell-system
#6
Aldi Pupuleku, Marcel Costa-García, Domènec Farré, Hartmut Hengel, Ana Angulo, Aura Muntasell, Miguel López-Botet
Human cytomegalovirus (HCMV) infection promotes the differentiation and persistent expansion of a mature NK cell subset, which displays high surface levels of the activating CD94/NKG2C NK cell receptor, together with additional distinctive phenotypic and functional features. The mechanisms underlying the development of adaptive NK cells remain uncertain but some observations support the involvement of a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV-infected cells. To approach this issue, the heterodimer and its adaptor (DAP12) were expressed in the human Jurkat leukemia T cell line; signaling was detected by transfection of a reporter plasmid encoding for Luciferase (Luc) under NFAT/AP1-dependent control...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29104792/three-anatomical-levels-possibilities-to-decrease-invasiveness-of-reconstructive-surgery-for-congenital-penile-curvature
#7
Wojciech Perdzyński, Marek Adamek
Introduction: The aim of the study was to report methods - based on penile anatomy - leading to the minimization in the invasiveness of reconstructive surgery for congenital penile curvature (CPC). Material and methods: From 2006 to 2016 authors operated on 186 adult men with CPC.To avoid degloving, the authors used the longitudinal skin and tunica dartos incision. For decreasing invasiveness to the dorsal neurovascular bundle (NVB), the authors separated it only locally in the shape of a triangle or a trapezium, elevating it only over the place of the tunical reconstruction...
2017: Central European Journal of Urology
https://www.readbyqxmd.com/read/29100326/fiber-modified-hexon-chimeric-oncolytic-adenovirus-targeting-cancer-associated-fibroblasts-inhibits-tumor-growth-in-gastric-carcinoma
#8
Tao Pang, Xinghua Wang, Jun Gao, Wei Chen, Xiao Jun Shen, Ming Ming Nie, Tianhang Luo, Kai Yin, Guoen Fang, Kai Xuan Wang, Xu Chao Xue
Objective: To evaluate the effects of fiber-modified hexon-chimeric recombinant oncolytic adenovirus targeting cancer associated fibroblasts (CAFs) on the gastric CAFs and the transplantation tumor mice model of gastric carcinoma (GC). Results: Compared with BJ cells and GPFs, the reproduction and infectivity of P9, P9-4C or GP adenoviruses were markedly higher in gastric CAFs. In addition, P9, P9-4C or GP had a significantly relatively more killing effect on gastric CAFs compared with GPFs, and have less oncolytic effect in BJ cells...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29084373/residual-sodium-dodecyl-sulfate-in-decellularized-muscle-matrices-leads-to-fibroblast-activation-in-vitro-and-foreign-body-response-in-vivo
#9
Emily E Friedrich, Steven T Lanier, Soleil Niknam-Bienia, Gabriel A Arenas, Divya Rajendran, Jason A Wertheim, Robert D Galiano
Detergents such as sodium dodecyl sulfate (SDS) are commonly used to extract cells from tissues in a process called 'decellularization'. Residual SDS is difficult to completely remove and may lead to an undesirable host response toward an implanted biomaterial. In this study, we developed a modification for SDS cell extraction from muscle equally efficient to previous methods but leading to significantly less residual SDS remnants in the matrices. Muscle-derived matrices were prepared via two SDS-based decellularization methods, which led to removal of either 81...
October 30, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29071143/the-prevalence-of-an-excessive-prepuce-and-the-effects-of-distal-circumcision-on-premature-ejaculation
#10
Luigi Gallo
OBJECTIVE: To investigate the prevalence of an excessive prepuce in patients with premature ejaculation (PE) and to evaluate the effectiveness of distal circumcision in reducing the penile hypersensitivity, which is thought to be a cause of PE. PATIENTS AND METHODS: Men were considered to have an excessive prepuce if the foreskin exceeded the external urethral meatus by ≥1 cm in the flaccid state. The diagnosis of PE was based on the Premature Ejaculation Diagnostic Tool (PEDT) questionnaire score and on the intravaginal ejaculatory latency time (IELT)...
June 2017: Arab Journal of Urology
https://www.readbyqxmd.com/read/29064609/the-micronome-of-mesenchymal-stromal-cells-is-partially-responsive-to-inflammation
#11
Hussein Fayyad-Kazan, Mohammad Fayyad-Kazan, Makram Merimi, Nathalie Meuleman, Dominique Bron, Laurence Lagneaux, Mehdi Najar
Mesenchymal stromal cells (MSCs) display a special immunological profile that allows their potential use as immunotherapeutic cells. Nowadays, Foreskin (FSK) represents a valuable reservoir of MSCs with International Society for cellular Therapy (ISCT) compliant criteria and relevant functional properties. However, their mode of action is poorly understood and needs to be more elucidated to optimize their therapeutic use. Because microRNAs (miRNAs) act as key regulators in a wide variety of biological processes, we decided to establish the micronome of FSK-MSCs, the influence of inflammation and the predicted target pathways...
October 24, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29062672/successful-second-microsurgical-replantation-for-amputated-penis
#12
Masahide Fujiki, Mine Ozaki, Akiko Kai, Akihiko Takushima, Kiyonori Harii
Penile amputation is a rare emergency, but the best method for its repair is required due to the organ's functional and societal role. Since the first successful microsurgical replantation of the amputated penis, microsurgical techniques have matured and become the standard treatment for the penile replantation. However, the successful second microsurgical replantation for amputated penis has been rarely reported. We present the case of a 40-year-old man with schizophrenia who had a past history of penile self-mutilation and successful replantation at another hospital 2 years ago...
September 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29044628/possible-pro-inflammatory-role-of-heparin-binding-epidermal-growth-factor-like-growth-factor-in-the-active-phase-of-systemic-sclerosis
#13
Megumi Hirabayashi, Yoshihide Asano, Takashi Yamashita, Shunsuke Miura, Kouki Nakamura, Takashi Taniguchi, Ryosuke Saigusa, Takehiro Takahashi, Yohei Ichimura, Takuya Miyagawa, Ayumi Yoshizaki, Tomomitsu Miyagaki, Makoto Sugaya, Shinichi Sato
Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is a member of the EGF family growth factors, which affects multiple aspects of the wound healing process such as epithelialization, wound contraction and angiogenesis. In our study, we measured the serum HB-EGF levels of 51 systemic sclerosis (SSc) patients, which showed a significant increase compared with those of 20 normal subjects. Further analysis revealed a positive correlation between the HB-EGF level and pulmonary ground-glass score but no correlation between the former and pulmonary fibrosis score...
October 17, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/29021657/results-of-tubularized-urethral-plate-urethroplasty-in-megameatus-intact-prepuce
#14
Amilal Bhat, Mahakshit Bhat, Akshita Bhat, Vikash Singh
OBJECTIVE: The megameatus variant of anterior hypospadias with an intact complete foreskin occurs in approximately 1%-3% of hypospadias. Hence, the objective of the study was to evaluate the results of tubularized urethral plate urethroplasty (TUPU) in megameatus intact prepuce (MIP). MATERIALS AND METHODS: A retrospective study (June 1996-June 2015) of MIP from our hypospadias registry was conducted. All patients with megameatus, either with an intact prepuce or with one previously removed, were included in the study...
October 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/29017952/a-biocompatible-sodium-alginate-povidone-iodine-film-enhances-wound-healing
#15
Maria Summa, Debora Russo, Ilaria Penna, Natasha Margaroli, Ilker S Bayer, Tiziano Bandiera, Athanassia Athanassiou, Rosalia Bertorelli
In the last few years, there has been an increasing tendency to use natural polymers for the fabrication of dressings for wound and burn management. Among them, alginate, a polysaccharide extracted primarily from marine algae, exhibits attractive properties being non-toxic, hydrophilic and biodegradable. The aim of this study was to characterize the in vitro biocompatibility and the efficacy of a composite polymeric material based on sodium alginate (NaAlg) and povidone iodine (PVPI) complex in a mouse model of wound healing...
October 7, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28938587/transcriptomic-profiles-of-human-foreskin-fibroblast-cells-in-response-to-orf-virus
#16
Daxiang Chen, Mingjian Long, Bin Xiao, Yufeng Xiong, Huiqin Chen, Yu Chen, Zhenzhan Kuang, Ming Li, Yingsong Wu, Daniel L Rock, Daoyuan Gong, Yong Wang, Haijian He, Fang Liu, Shuhong Luo, Wenbo Hao
Orf virus has been utilized as a safe and efficient viral vector against not only diverse infectious diseases, but also against tumors. However, the nature of the genes triggered by the vector in human cells is poorly characterized. Using RNA sequencing technology, we compared specific changes in the transcriptomic profiles in human foreskin fibroblast cells following infection by the orf virus. The results indicated that orf virus upregulates or downregulates expression of a variety of genes, including genes involved in antiviral immune response, apoptosis, cell cycle and a series of signaling pathways, such as the IFN and p53-signaling pathways...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28922561/reconciling-female-genital-circumcision-with-universal-human-rights
#17
John-Stewart Gordon
One of the most challenging issues in cross-cultural bioethics concerns the long-standing socio-cultural practice of female genital circumcision (FGC), which is prevalent in many African countries and the Middle East as well as in some Asian and Western countries. It is commonly assumed that FGC, in all its versions, constitutes a gross violation of the universal human rights of health, physical integrity, and individual autonomy and hence should be abolished. This article, however, suggests a mediating approach according to which one form of FGC, the removal of the clitoris foreskin, can be made compatible with the high demands of universal human rights...
September 18, 2017: Developing World Bioethics
https://www.readbyqxmd.com/read/28918366/a-progressive-reduction-in-autophagic-capacity-contributes-to-induction-of-replicative-senescence-in-hs68-cells
#18
Byeal-I Han, Sung-Hee Hwang, Michael Lee
Autophagy has been implicated in delayed aging and extended longevity. Here, we aimed to study the possible effects of autophagy during the progression of replicative senescence, which is one of the major features of aging. Human foreskin fibroblasts, Hs68 cells, at an initial passage of 15 were serially cultured for several months until they reached cellular senescence. A decrease in cell proliferation was observed during the progression of senescence. Induction of replicative senescence in aged cells (at passage 40) was confirmed by senescence-associated β-galactosidase (SA-β-gal) activity that represents a sensitive and reliable marker for quantifying senescent cells...
September 14, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28912571/comparative-global-immune-related-gene-profiling-of-somatic-cells-human-pluripotent-stem-cells-and-their-derivatives-implication-for-human-lymphocyte-proliferation
#19
Chia-Eng Wu, Chen-Wei Yu, Kai-Wei Chang, Wen-Hsi Chou, Chen-Yu Lu, Elisa Ghelfi, Fang-Chun Wu, Pey-Shynan Jan, Mei-Chi Huang, Patrick Allard, Shau-Ping Lin, Hong-Nerng Ho, Hsin-Fu Chen
Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), represent potentially unlimited cell sources for clinical applications. Previous studies have suggested that hPSCs may benefit from immune privilege and limited immunogenicity, as reflected by the reduced expression of major histocompatibility complex class-related molecules. Here we investigated the global immune-related gene expression profiles of human ESCs, hiPSCs and somatic cells and identified candidate immune-related genes that may alter their immunogenicity...
September 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28910076/gold-decorated-porous-silicon-nanopillars-for-targeted-hyperthermal-treatment-of-bacterial-infections
#20
Hashim Alhmoud, Anna Cifuentes-Rius, Bahman Delalat, David G Lancaster, Nicolas H Voelcker
In order to address the issue of pathogenic bacterial colonization of diabetic wounds, a more direct and robust approach is required, which relies on a physical form of bacterial destruction in addition to the conventional biochemical approach (i.e., antibiotics). Targeted bacterial destruction through the use of photothermally active nanomaterials has recently come into the spotlight as a viable approach to solving the rising problem of antibiotic resistant microorganisms. Materials with high absorption coefficients in the near-infrared (NIR) region of the electromagnetic spectrum show promise as alternative antibacterial therapeutic agents, since they preclude the development of bacterial resistance and can be activated on demand...
October 4, 2017: ACS Applied Materials & Interfaces
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