keyword
MENU ▼
Read by QxMD icon Read
search

Saturation screening

keyword
https://www.readbyqxmd.com/read/29331035/noninvasive-optical-spectroscopy-for-identification-of-non-melanoma-skin-cancer-pilot-study
#1
David J Carpenter, Mirabelle B Sajisevi, Nikita Chapurin, Clifford Scott Brown, Tracy Cheng, Gregory M Palmer, Daniel S Stevenson, Caroline L Rao, Russell P Hall, Charles R Woodard
OBJECTIVE: Optical spectroscopy offers a noninvasive alternative to biopsy as a first-line screening tool for suspicious skin lesions. This study sought to define several optical parameters across malignant and benign tissue types. STUDY DESIGN: Prospective pilot trial utilizing the Zenalux IM1 optical spectroscopy device from April 2016 to February 2017. For each skin lesion, provider pre-biopsy probability of malignancy was compared to histolopathologic diagnosis...
January 13, 2018: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29327758/a-computational-analysis-of-pro-angiogenic-therapies-for-peripheral-artery-disease
#2
Lindsay E Clegg, Feilim Mac Gabhann
Inducing therapeutic angiogenesis to effectively form hierarchical, non-leaky networks of perfused vessels in tissue engineering applications and ischemic disease remains an unmet challenge, despite extensive research and multiple clinical trials. Here, we use a previously-developed, multi-scale, computational systems pharmacology model of human peripheral artery disease to screen a diverse array of promising pro-angiogenic strategies, including gene therapy, biomaterials, and antibodies. Our previously-validated model explicitly accounts for VEGF immobilization, Neuropilin-1 binding, and weak activation of VEGF receptor 2 (VEGFR2) by the "VEGFxxxb" isoforms...
January 12, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29320532/automatic-outer-and-inner-breast-tissue-segmentation-using-multi-parametric-mri-images-of-breast-tumor-patients
#3
Snekha Thakran, Subhajit Chatterjee, Meenakshi Singhal, Rakesh Kumar Gupta, Anup Singh
The objectives of the study were to develop a framework for automatic outer and inner breast tissue segmentation using multi-parametric MRI images of the breast tumor patients; and to perform breast density and tumor tissue analysis. MRI of the breast was performed on 30 patients at 3T-MRI. T1, T2 and PD-weighted(W) images, with and without fat saturation(WWFS), and dynamic-contrast-enhanced(DCE)-MRI data were acquired. The proposed automatic segmentation approach was performed in two steps. In step-1, outer segmentation of breast tissue from rest of body parts was performed on structural images (T2-W/T1-W/PD-W without fat saturation images) using automatic landmarks detection technique based on operations like profile screening, Otsu thresholding, morphological operations and empirical observation...
2018: PloS One
https://www.readbyqxmd.com/read/29316672/aquaculture-soft-coral-lobophytum-crassum-as-a-producer-of-anti-proliferative-cembranoids
#4
Bo-Rong Peng, Mei-Chin Lu, Mohamed El-Shazly, Shwu-Li Wu, Kuei-Hung Lai, Jui-Hsin Su
Our continuous search for marine bioactive secondary metabolites led to the screening of crude extracts from a variety of aquaculture soft corals. The ethyl acetate (EtOAc) extract of Lobophytum crassum showed a distinctive chemical profile that was different from the wild type. It demonstrated significant anti-proliferative activity against Molt 4 leukemia cell with an IC50 value of 1 μg/mL after 24 h. Chemical investigation focusing on the unique peaks in L. crassum profile led to the discovery of a new α-tocopherol crassumtocopherol C (1), and two new cembrane-based diterpenoids culobophylins D (2) and E (3), along with ten known cembranoids (4-13)...
January 7, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29314451/methodology-development-in-directed-evolution-exploring-options-when-applying-triple-code-saturation-mutagenesis
#5
Ge Qu, Richard Lonsdale, Peiyuan Yao, Guangyue Li, Beibei Liu, Manfred T Reetz, Zhoutong Sun
Directed evolution of stereo- or regioselective enzymes as catalysts in asymmetric transformations is of particular interest in organic synthesis. Upon evolving these biocatalysts, screening is the bottleneck. To beat the numbers problem most effectively, methods and strategies for building "small but smart" mutant libraries have been developed. Herein, we compared two different strategies regarding the application of triple-code saturation mutagenesis (TCSM) at multiresidue sites of the Thermoanaerobacter brockii alcohol dehydrogenase by using distinct reduced amino-acid alphabets...
January 4, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29307512/the-endoc-%C3%AE-h1-cell-line-is-a-valid-model-of-human-beta-cells-and-applicable-for-screenings-to-identify-novel-drug-target-candidates
#6
Violeta Georgieva Tsonkova, Fredrik Wolfhagen Sand, Xenia Asbæk Wolf, Lars Groth Grunnet, Anna Kirstine Ringgaard, Camilla Ingvorsen, Louise Winkel, Mark Kalisz, Kevin Dalgaard, Christine Bruun, Johannes Josef Fels, Charlotte Helgstrand, Sven Hastrup, Fredrik Kryh Öberg, Erik Vernet, Michael Paolo Bastner Sandrini, Allan Christian Shaw, Carsten Jessen, Mads Grønborg, Jacob Hald, Hanni Willenbrock, Dennis Madsen, Rasmus Wernersson, Lena Hansson, Jan Nygaard Jensen, Annette Plesner, Tomas Alanentalo, Maja Borup Kjær Petersen, Anne Grapin-Botton, Christian Honoré, Jonas Ahnfelt-Rønne, Jacob Hecksher-Sørensen, Philippe Ravassard, Ole D Madsen, Claude Rescan, Thomas Frogne
OBJECTIVE: To characterize the EndoC-βH1 cell line as a model for human beta cells and evaluate its beta cell functionality, focusing on insulin secretion, proliferation, apoptosis and ER stress, with the objective to assess its potential as a screening platform for identification of novel anti-diabetic drug candidates. METHODS: EndoC-βH1 was transplanted into mice for validation of in vivo functionality. Insulin secretion was evaluated in cells cultured as monolayer and as pseudoislets, as well as in diabetic mice...
December 19, 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/29296481/in-vivo-real-time-imaging-of-cutaneous-hemoglobin-concentration-oxygen-saturation-scattering-properties-melanin-content-and-epidermal-thickness-with-visible-spatially-modulated-light
#7
Xinlin Chen, Weihao Lin, Chenge Wang, Shaoheng Chen, Jing Sheng, Bixin Zeng, M Xu
We present the real-time single snapshot multiple frequency demodulation - spatial frequency domain imaging (SSMD-SFDI) platform implemented with a visible digital mirror device that is capable of imaging and monitoring dynamic turbid medium and processes over a large field of view. One challenge in quantitative imaging of biological tissue such as the skin is the complex structure rendering techniques based on homogeneous medium models to fail. To address this difficulty we have also developed a novel method that maps the layered structure to a homogeneous medium for spatial frequency domain imaging...
December 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29285649/carboxyhemoglobin-levels-induced-by-cigarette-smoking-outdoors-in-smokers
#8
Jonathan Schimmel, Naomi George, John Schwarz, Sami Yousif, Selim Suner, Jason B Hack
INTRODUCTION: Non-invasive screening of carboxyhemoglobin saturation (SpCO) in the emergency department to detect occult exposure is increasingly common. The SpCO threshold to consider exposure in smokers is up to 9%. The literature supporting this cutoff is inadequate, and the impact of active smoking on SpCO saturation remains unclear. The primary objective was to characterize baseline SpCO in a cohort of smokers outdoors. Secondary objectives were to explore the impact of active smoking on SpCO and to compare SpCO between smokers and non-smokers...
December 28, 2017: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
https://www.readbyqxmd.com/read/29280293/subclinical-hypothyroidism-a-common-finding-in-adult-patients-with-cyanotic-congenital-heart-disease
#9
Peter Bak, Cristel S Hjortshøj, Peter Gaede, Lars Idorn, Lars Søndergaard, Annette S Jensen
OBJECTIVE: Cyanotic congenital heart disease is a systemic disease, with effects on multiple organ systems. A high prevalence of subclinical hypothyroidism (SCH) has been reported in a small cohort of cyanotic congenital heart disease patients. Subclinical hypothyroidism has been associated with various adverse cardiovascular effects, as well as an increased risk of progression to overt hypothyroidism. The aim of this study was to examine the prevalence of SCH in cyanotic congenital heart disease patients, consider possible etiologies, and evaluate thyroid function over time...
December 27, 2017: Congenital Heart Disease
https://www.readbyqxmd.com/read/29273653/perfusion-index-in-healthy-newborns-during-critical-congenital-heart-disease-screening-at-24-hours-retrospective-observational-study-from-the-usa
#10
Priya Jegatheesan, Matthew Nudelman, Keshav Goel, Dongli Song, Balaji Govindaswami
OBJECTIVE: To describe the distribution of perfusion index (PI) in asymptomatic newborns at 24 hours of life when screening for critical congenital heart disease (CCHD) using an automated data selection method. DESIGN: This is a retrospective observational study. SETTING: Newborn nursery in a California public hospital with ~3500 deliveries annually. METHODS: We developed an automated programme to select the PI values from CCHD screens...
December 21, 2017: BMJ Open
https://www.readbyqxmd.com/read/29273507/organic-anion-transporter-1-oat1-slc22a6-enhances-bioluminescence-based-on-d-luciferin-luciferase-reaction-in-living-cells-by-facilitating-the-intracellular-accumulation-of-d-luciferin
#11
Takahito Furuya, Issey Takehara, Asuka Shimura, Hisanao Kishimoto, Tomoya Yasujima, Kinya Ohta, Yoshiyuki Shirasaka, Hiroaki Yuasa, Katsuhisa Inoue
Bioluminescence (BL) imaging based on d-luciferin (d-luc)-luciferase reaction allows noninvasive and real-time monitoring of luciferase-expressing cells. Because BL intensity depends on photons generated through the d-luc-luciferase reaction, an approach to increase intracellular levels of d-luc could improve the detection sensitivity. In the present study, we showed that organic anion transporter 1 (OAT1) is useful, as a d-luc transporter, in boosting the BL intensity in luciferase-expressing cells. Functional screening of several transporters showed that the expression of OAT1 in HEK293 cells stably expressing Pyrearinus termitilluminans luciferase (HEK293/eLuc) markedly enhanced BL intensity in the presence of d-luc...
December 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29250094/variability-of-pyrrolizidine-alkaloid-occurrence-in-species-of-the-grass-subfamily-pooideae-poaceae
#12
Anne-Maria Wesseling, Tobias J Demetrowitsch, Karin Schwarz, Dietrich Ober
Pyrrolizidine alkaloids (PAs) are a class of secondary metabolites found in various unrelated angiosperm lineages including cool-season grasses (Poaceae, subfamily Pooideae). Thesinine conjugates, saturated forms of PA that are regarded as non-toxic, have been described to occur in the two grass species Lolium perenne and Festuca arundinacea (Poaceae, subfamily Pooideae). In a wider screen, we tested various species of the Pooideae lineage, grown under controlled conditions, for their ability to produce thesinine conjugates or related structures...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29246586/diagnostic-per-lesion-performance-of-a-simulated-gadoxetate-disodium-enhanced-abbreviated-mri-protocol-for-hepatocellular-carcinoma-screening
#13
B G Tillman, J D Gorman, J M Hru, M H Lee, M C King, C B Sirlin, R M Marks
AIM: To evaluate the diagnostic per-lesion performance of a simulated gadoxetate disodium-enhanced abbreviated MRI (AMRI) in cirrhotic and chronic hepatitis B (CHB) patients for hepatocellular carcinoma (HCC) screening. MATERIALS AND METHODS: Seventy-nine consecutive patients at risk for HCC due to cirrhosis and/or CHB were included in this retrospective study. For each patient, the first gadoxetate disodium-enhanced MRI between 2008 through 2014 was analysed. Two independent readers read an anonymised abbreviated image set comprising axial T1-weighted (W) images with fat saturation in the hepatobiliary phase, 20 minutes or more after gadoxetate injection, and axial T2W single-shot fast spin echo images...
December 12, 2017: Clinical Radiology
https://www.readbyqxmd.com/read/29237725/abin-1-negatively-regulates-%C3%AE-opioid-receptor-function
#14
Peilan Zhou, Jiebing Jiang, Hui Yan, Yulei Li, Junru Zhao, Xiao Wang, Ruibin Su, Zehui Gong
The μ-opioid receptor (MOR) is a Gi/o protein-coupled receptor that mediates analgesic, euphoric, and reward effects. Using a bacterial two-hybrid screen, we reported that the carboxyl tail of the rat MOR associates with A20-binding inhibitor of nuclear factor (NF)-κB (ABIN-1) (Zhou et al., 2015). This interaction was confirmed by direct protein - protein binding and co-immunoprecipitation of MOR and ABIN-1 proteins in cell lysates. Saturation binding studies showed that ABIN-1 had no effect on MOR binding...
December 13, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/29236321/murine-models-of-osteosarcoma-a-piece-of-the-translational-puzzle
#15
Mannu K Walia, Wilson Castillo-Tandazo, Anthony J Mutsaers, T John Martin, Carl R Walkley
Osteosarcoma (OS) is the most common cancer of bone in children and young adults. Despite extensive research efforts, there has been no significant improvement in patient outcome for many years. An improved understanding of the biology of this cancer and how genes frequently mutated contribute to OS may help improve outcomes for patients. Whilst our knowledge of the mutational burden of OS is approaching saturation, our understanding of how these mutations contribute to OS initiation and maintenance is less clear...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29232521/an-immunoglobulin-fc-fused-peptide-without-c-terminal-arg-or-lys-residue-augments-neuropilin-1-dependent-tumor-vascular-permeability
#16
Du-San Baek, Jeong-Ho Kim, Ye-Jin Kim, Yong-Sung Kim
Neuropilin-1 (NRP1), which functions as a co-receptor for vascular endothelial growth factor (VEGF) and is implicated in vascular permeability and tumorigenesis, has been targeted by peptides that specifically bind to the VEGF-binding region on NRP1. Like natural VEGF ligands, all known peptides with NRP1-binding activity bind only through a carboxy (C)-terminal R/K-x-x-R/K sequence motif (x stands for any amino acids); this strict requirement is called the C-end rule (CendR). Here, we report immunoglobulin Fc-fused NRP1-specific peptides deviating from CendR...
December 12, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29225193/synthesis-and-in-vitro-characterization-of-a-p2%C3%A3-7-radioligand-123i-tz6019-and-its-response-to-neuroinflammatory-in-a-mouse-model-of-alzheimer-disease
#17
Hongjun Jin, Junbin Han, Derek Resing, Hui Liu, Xuyi Yue, Rebecca L Miller, Kathleen M Schoch, Timothy M Miller, Joel S Perlmutter, Terrance M Egan, Zhude Tu
The purinergic receptor P2X ligand-gated ion channel 7 (P2×7 receptor) is a promising imaging target to detect neuroinflammation. Herein, we report development of a potent iodinated labeled radiotracer for P2×7 receptor, [123I]TZ6019. The radiosynthesis of [123I]TZ6019 was accomplished by allylic-tin precursor iodination using [123I]NaI with good radiochemical yield of 85% and high radiochemical purity of > 99%. Human embryonic kidney 293 (HEK-293) cell line stably transfected with the human P2×7 receptor was used to characterize the binding affinity of TZ6019 in fluorescence, radioactive competitive, and saturation binding assays...
December 7, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29215906/parental-perspectives-of-screening-for-adverse-childhood-experiences-in-pediatric-primary-care
#18
Anne-Marie Conn, Moira A Szilagyi, Sandra H Jee, Jody T Manly, Rahil Briggs, Peter G Szilagyi
INTRODUCTION: Pediatricians recognize a need to mitigate the negative impact that adverse childhood experiences (ACEs) can have on health and development. However, ACEs screening and interventions in primary care pediatrics may be inhibited by concerns about parental perceptions. We assessed parent perspectives of screening for ACEs in the pediatric primary care setting, to understand their views on the potential impact of their ACEs on their parenting and to identify opportunities for pediatric anticipatory guidance...
December 7, 2017: Families, Systems & Health: the Journal of Collaborative Family Healthcare
https://www.readbyqxmd.com/read/29214652/pharmacology-in-translation-the-preclinical-and-early-clinical-profile-of-the-novel-%C3%AE-2-3-functionally-selective-gabaa-receptor-positive-allosteric-modulator-pf-06372865
#19
Sarah A Nickolls, Rachel Gurrell, Guido van Amerongen, Juha Kammonen, Lishuang Cao, Adam R Brown, Clara Stead, Andy Mead, Christine Watson, Cathleen Hsu, Robert M Owen, Andy Pike, Rebecca L Fish, Laigao Chen, Ruolun Qiu, Evan D Morris, Gang Feng, Mark Whitlock, Donal Gorman, Joop van Gerven, David S Reynolds, Pinky Dua, Richard P Butt
BACKGROUND AND PURPOSE: Benzodiazepines (BZDs), non-selective positive allosteric modulators (PAMs) of GABAA receptors, have significant side-effects that limit their clinical utility. As many of these side-effects are mediated by the α1 subunit there has been a concerted effort to develop α2/3 subtype-selective PAMs. EXPERIMENTAL APPROACH: In vitro screening assays were used to identify molecules with functional selectivity for receptors containing α2/3 subunits over those containing α1 subunits...
December 7, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29205091/polysomnographic-oxygen-saturation-findings-for-preteen-children-versus-adolescents
#20
Nathan D Wiebracht, Shan He, Colin Cotton, Jareen Meinzen-Derr, Gordon Shott, David F Smith, Keith B McConnell, Stacey L Ishman
Objective Home oximetry is commonly used to screen for obstructive sleep apnea (OSA) in children; however, normal oxygen desaturation levels by disease severity are not well known. It was our objective to determine if oxygen saturation levels differed by OSA severity category in children and if these differences were similar for preteen children and adolescents. Study Design Retrospective case series of children undergoing polysomnography from September 2011 to July 2015. Setting Tertiary pediatric hospital...
November 1, 2017: Otolaryngology—Head and Neck Surgery
keyword
keyword
12061
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"