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Saturation screening

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https://www.readbyqxmd.com/read/27904846/complete-congenital-heart-block-in-a-neonate-with-a-complex-congenital-heart-defect-in-africa
#1
Clovis Nkoke, Edvine Yonta Wawo, Liliane Kuate Mfeukeu, Larissa Makamte, Sandrine Dikosso Edie, Flore Esiene Balana
Congenital heart block (CHB) is rare disorder that has a higher mortality when associated with structural congenital heart defects. Very few cases have been reported in Sub-Saharan Africa (SSA). We present a case of complete CHB associated with a complex congenital heart defect in a neonate in Cameroon. A 1-month-old neonate in Cameroon was referred for the evaluation of bradycardia. The obstetrical ultrasound done during pregnancy revealed fetal bradycardia without further evaluation. Clinical examination showed well a developed neonate with bradycardia at 62 beats/minute, and mild cyanosis with oxygen saturation at 93% at room air...
October 2016: Cardiovascular Diagnosis and Therapy
https://www.readbyqxmd.com/read/27893829/sansevieria-roxburghiana-schult-schult-f-family-asparagaceae-attenuates-type-2-diabetes-and-its-associated-cardiomyopathy
#2
Niloy Bhattacharjee, Ritu Khanra, Tarun K Dua, Susmita Das, Bratati De, M Zia-Ul-Haq, Vincenzo De Feo, Saikat Dewanjee
BACKGROUND: Sansevieria roxburghiana Schult. & Schult. F. (Family: Asparagaceae) rhizome has been claimed to possess antidiabetic activity in the ethno-medicinal literature in India. Therefore, present experiments were carried out to explore the protective role of edible (aqueous) extract of S. roxburghiana rhizome (SR) against experimentally induced type 2 diabetes mellitus (T2DM) and its associated cardiomyopathy in Wistar rats. METHODS: SR was chemically characterized by GC-MS analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27890299/monte-carlo-simulations-of-the-gamma-ray-exposure-rates-of-common-rocks
#3
Daniel A Haber, Russell L Malchow, Pamela C Burnley
Monte Carlo simulations have been performed to model the gamma ray emission and attenuation properties of common rocks. In geologic materials, (40)K, (238)U, and (232)Th are responsible for most gamma ray production. If the concentration of these radioelements and attenuation factors such as degree of water saturation are known, an estimate of the gamma-ray exposure rate can be made. The results show that there are no significant differences in gamma-ray screening between major rock types. If the total number of radionuclide atoms are held constant then the major controlling factor is density of the rock...
November 24, 2016: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/27888307/monitoring-doxorubicin-cellular-uptake-and-trafficking-using-in-vitro-raman-microspectroscopy-short-and-long-time-exposure-effects-on-lung-cancer-cell-lines
#4
Zeineb Farhane, Franck Bonnier, Hugh J Byrne
Raman microspectroscopy is a non-invasive, in vitro analytical tool which is being increasingly explored for its potential in clinical applications and monitoring the uptake, mechanism of action and cellular interaction at a molecular level of chemotherapeutic drugs, ultimately as a potential label-free preclinical screening and companion diagnostic tool. In this study, doxorubicin (DOX), a "gold standard" chemotherapeutic drug, is employed as a model in the in vitro lung cancer cell line A549 in order to demonstrate the potential of Raman microspectroscopy to screen and identify spectroscopic markers of its trafficking and mechanism of action...
November 25, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27887027/expansion-of-the-substrate-range-of-the-gentisate-1-2-dioxygenase-from-corynebacterium-glutamicum-for-the-conversion-of-monohydroxylated-benzoates
#5
Erik Eppinger, Andreas Stolz
The gentisate 1,2-dioxygenases (GDOs) from Corynebacterium glutamicum and various other organisms oxidatively cleave the aromatic nucleus of gentisate (2,5-dihydroxybenzoate), but are not able to convert salicylate (2-hydroxybenzoate). In contrast, the α-proteobacterium Pseudaminobacter salicylatoxidans synthesises an enzyme ('salicylate dioxygenase', SDO) which cleaves gentisate, but also (substituted) salicylate(s). Sequence comparisons showed that the SDO belongs to a group of GDOs mainly originating from Gram-positive bacteria which also include the GDO from C...
November 24, 2016: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/27885598/identification-of-ligand-receptor-interactions-ligand-molecular-arrays-spr-and-nmr-methodologies
#6
Christopher J Day, Lauren E Hartley-Tassell, Victoria Korolik
Despite many years of research into bacterial chemotaxis, the only well characterized system to date is that of E. coli. Even for E. coli, the direct ligand binding had been fully characterized only for aspartate and serene receptors Tar and Tsr. In 30 years since, no other direct receptor-ligand interaction had been described for bacteria, until the characterization of the C. jejuni aspartate and multiligand receptors (Hartley-Tassell et al. Mol Microbiol 75:710-730, 2010). While signal transduction components of many sensory pathways have now been characterized, ligand-receptor interactions remain elusive due to paucity of high-throughput screening methods...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27869117/genome-wide-rnai-screen-reveals-alk1-mediates-ldl-uptake-and-transcytosis-in-endothelial-cells
#7
Jan R Kraehling, John H Chidlow, Chitra Rajagopal, Michael G Sugiyama, Joseph W Fowler, Monica Y Lee, Xinbo Zhang, Cristina M Ramírez, Eon Joo Park, Bo Tao, Keyang Chen, Leena Kuruvilla, Bruno Larriveé, Ewa Folta-Stogniew, Roxana Ola, Noemi Rotllan, Wenping Zhou, Michael W Nagle, Joachim Herz, Kevin Jon Williams, Anne Eichmann, Warren L Lee, Carlos Fernández-Hernando, William C Sessa
In humans and animals lacking functional LDL receptor (LDLR), LDL from plasma still readily traverses the endothelium. To identify the pathways of LDL uptake, a genome-wide RNAi screen was performed in endothelial cells and cross-referenced with GWAS-data sets. Here we show that the activin-like kinase 1 (ALK1) mediates LDL uptake into endothelial cells. ALK1 binds LDL with lower affinity than LDLR and saturates only at hypercholesterolemic concentrations. ALK1 mediates uptake of LDL into endothelial cells via an unusual endocytic pathway that diverts the ligand from lysosomal degradation and promotes LDL transcytosis...
November 21, 2016: Nature Communications
https://www.readbyqxmd.com/read/27864781/isolation-identification-and-high-throughput-screening-of-neutral-lipid-producing-indigenous-microalgae-from-south-african-aquatic-habitats
#8
S T Gumbi, B M Majeke, A O Olaniran, T Mutanda
Exploring indigenous microalgae capable of producing significant amounts of neutral lipids through high-throughput screening is crucial for sustainable biodiesel production. In this study, 31 indigenous microalgal strains were isolated from diverse aquatic habitats in KwaZulu-Natal, South Africa. Eight superior lipid-producing strains were selected for further analysis, based on Nile red fluorescence microscopy screening. The microalgal isolates were identified to belong to the genera Chlorella, Neochloris and Chlamydomonas via morpho-taxonomic and molecular approach by 18S rRNA gene sequencing...
November 18, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27864059/biosynthesis-and-physicochemical-characterization-of-a-bacterial-polysaccharide-polyamide-blend-applied-for-microfluidics-study-in-porous-media
#9
Maryam Ijadi Bajestani, Seyyed Mohammad Mousavi, Arezou Jafari, Seyed Abbas Shojaosadati
Screening among some new isolated bacteria from oily samples, which were capable of producing extracellular polymeric substances (EPSs), one was selected and identified as Bacillus sonorensis. An efficient micro-total analysis approach was carried out to assay the produced EPSs by this bacterium. Sucrose and yeast concentrations as carbon and nitrogen sources, respectively, sodium salt concentration and initial pH were selected to be the variables in experimental design. Production of EPS in optimal condition was increased by 5...
November 15, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27847673/site-saturation-mutagenesis-applications-on-candida-methylica-formate-dehydrogenase
#10
Gülşah P Özgün, Emel B Ordu, H Esra Tütüncü, Emrah Yelboğa, Richard B Sessions, Nevin Gül Karagüler
In NADH regeneration, Candida methylica formate dehydrogenase (cmFDH) is a highly significant enzyme in pharmaceutical industry. In this work, site saturation mutagenesis (SSM) which is a combination of both rational design and directed evolution approaches is applied to alter the coenzyme specificity of NAD(+)-dependent cmFDH from NAD(+) to NADP(+) and increase its thermostability. For this aim, two separate libraries are constructed for screening a change in coenzyme specificity and an increase in thermostability...
2016: Scientifica
https://www.readbyqxmd.com/read/27833014/protein-engineering-of-aldolase-lbdera-for-enhanced-activity-toward-real-substrates-with-a-high-throughput-screening-method-coupled-with-an-aldehyde-dehydrogenase
#11
Xue-Cheng Jiao, Jiang Pan, Xu-Dong Kong, Jian-He Xu
A new high-throughput method for screening 2-deoxyribose-5-phosphate aldolase variants with a higher activity toward aldol reaction of unnatural aldehydes was established for the first time by coupling with an aldehyde dehydrogenase LeADH. The error-prone PCR and site-directed saturation mutagenesis libraries of aldolase LbDERA were constructed and screened using the high-throughput method. Two improved variants, LbDERAT29L and LbDERAF163Y, were identified and combined, giving a double mutant LbDERAT29L/F163Y which showed 7-fold higher activity than the native enzyme...
November 7, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27831548/a-novel-more-efficient-staged-approach-for-critical-congenital-heart-disease-screening
#12
J Mouledoux, S Guerra, J Ballweg, Y Li, W Walsh
OBJECTIVE: Screening for critical congenital heart disease (CCHD) using pulse oximetry has been endorsed by the American Academy of Pediatrics and the American Heart Association. The recommended screening requires two saturation readings. We sought to determine the incidence of undetected CCHD in Tennessee for the 2 years following implementation of an algorithm that assigned an immediate pass to a single lower extremity saturation of 97% or higher. STUDY DESIGN: State Genetic Screening records and reports of missed cases from the Tennessee Initiative for Perinatal Quality Care were used to determine if CCHD cases were missed by the new screening algorithm...
November 10, 2016: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/27830294/single-mutations-of-ketoreductase-chkred20-enhance-the-bioreductive-production-of-1s-2-chloro-1-3-4-difluorophenyl-ethanol
#13
Feng-Jiao Zhao, Yan Liu, Xiao-Qiong Pei, Chao Guo, Zhong-Liu Wu
(1S)-2-chloro-1-(3, 4-difluorophenyl) ethanol ((S)-CFPL) is an intermediate for the drug ticagrelor, and is manufactured via chemical approaches. To develop a biocatalytic solution to (S)-CFPL, an inventory of ketoreductases from Chryseobacterium sp. CA49 were rescreened, and ChKRED20 was found to catalyze the reduction of the ketone precursor with excellent stereoselectivity (>99 % ee). After screening an error-prone PCR library of the wild-type ChKRED20, two mutants, each bearing a single amino acid substitution of H145L or L205M, were identified with significantly increased activity...
November 9, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27827901/structure-activity-relationship-studies-using-natural-and-synthetic-okadaic-acid-dinophysistoxin-toxins
#14
Michael J Twiner, Gregory J Doucette, Yucheng Pang, Chao Fang, Craig J Forsyth, Christopher O Miles
Okadaic acid (OA) and the closely related dinophysistoxins (DTXs) are algal toxins that accumulate in shellfish and are known serine/threonine protein phosphatase (ser/thr PP) inhibitors. Phosphatases are important modulators of enzyme activity and cell signaling pathways. However, the interactions between the OA/DTX toxins and phosphatases are not fully understood. This study sought to identify phosphatase targets and characterize their structure-activity relationships (SAR) with these algal toxins using a combination of phosphatase activity and cytotoxicity assays...
November 4, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27821715/a-novel-diet-induced-murine-model-of-steatohepatitis-with-fibrosis-for-screening-and-evaluation-of-drug-candidates-for-nonalcoholic-steatohepatitis
#15
Chieko Ejima, Haruna Kuroda, Sonoko Ishizaki
Many animal models of nonalcoholic steatohepatitis have been reported. While these models exhibit mild onset of hepatitis and fibrosis, induction is often slow. For faster screening of drug candidates, there is a compelling need for convenient animal models of steatohepatitis and nonalcoholic steatohepatitis in which fatty liver and hepatitis are stably induced within a short period. Here, we analyzed the hepatic lipid composition in nonalcoholic steatohepatitis, and used this information to successfully establish a murine model where steatohepatitis is induced within only 1 week using a novel diet (steatohepatitis-inducing high-fat diet, STHD-01) high in saturated fatty acids and cholesterol...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27811597/predictors-of-at-home-arterial-oxygen-desaturation-events-in-ambulatory-surgical-patients
#16
Chuck Biddle, Charles Elam, Laura Lahaye, Gordon Kerr, Laura Chubb, Brad Verhulst
OBJECTIVES: Little is known about the early recovery phase occurring at-home after anesthesia and surgery in ambulatory surgical patients. We studied quantitative oximetry and quality-of-life metrics in the first 48 hours after same-day orthopedic surgery examining the association between the recovery metrics and specific patient and procedural factors. METHODS: We used the STOP-Bang score to quantify patient risk for obstructive sleep apnea in 50 adult patients at 2 centers using continuous portable oximetry and patient journaling...
November 2, 2016: Journal of Patient Safety
https://www.readbyqxmd.com/read/27793673/lead-discovery-for-mammalian-elongation-of-long-chain-fatty-acids-family-6-using-a-combination-of-high-throughput-fluorescent-based-assay-and-rapidfire-mass-spectrometry-assay
#17
Mari Takamiya, Masaaki Sakurai, Fumie Teranishi, Tomoko Ikeda, Tsutomu Kamiyama, Akira Asai
A high-throughput RapidFire mass spectrometry assay is described for elongation of very long-chain fatty acids 6 (Elovl6). Elovl6 is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids. Elovl6 may be a new therapeutic target for fat metabolism disorders such as obesity, type 2 diabetes, and nonalcoholic steatohepatitis. To identify new Elovl6 inhibitors, we developed a high-throughput fluorescence screening assay in 1536-well format. However, a number of false positives caused by fluorescent interference have been identified...
October 25, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27792838/adsorption-of-shiga-toxin-to-poly-%C3%AE-glutamate-precipitated
#18
Tsukie Goto, Makiko Tsuji, Kaori Kanemaru, Kumio Yokoigawa
We screened foods containing indigestible ingredients in the ability to adsorb Shiga toxin (Stx). When 5 mg of foods and dietary fibers such as dry vegetables and inulin were mixed and incubated with 0.5 mL of Stx solution (100 ng/mL) containing 0.5% bovine serum albumin, both Stx1 and Stx2 seemed to be adsorbed by only a fermented food, natto (a traditional Japanese food prepared from steamed soybeans by the biological action of Bacillus subtilis). We purified the Stx-adsorbing substance from natto by extraction with H2 O, acid treatment, Proteinase K treatment, and an ion exchange chromatography...
October 28, 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27786320/dynamic-behaviour-of-the-silica-water-bio-electrical-double-layer-in-the-presence-of-a-divalent-electrolyte
#19
B M Lowe, Y Maekawa, Y Shibuta, T Sakata, C-K Skylaris, N G Green
Electronic devices are becoming increasingly used in chemical- and bio-sensing applications and therefore understanding the silica-electrolyte interface at the atomic scale is becoming increasingly important. For example, field-effect biosensors (BioFETs) operate by measuring perturbations in the electric field produced by the electrical double layer due to biomolecules binding on the surface. In this paper, explicit-solvent atomistic calculations of this electric field are presented and the structure and dynamics of the interface are investigated in different ionic strengths using molecular dynamics simulations...
October 27, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27782307/estimation-of-relative-free-energies-of-binding-using-pre-computed-ensembles-based-on-the-single-step-free-energy-perturbation-and-the-site-identification-by-ligand-competitive-saturation-approaches
#20
E Prabhu Raman, Sirish Kaushik Lakkaraju, Rajiah Aldrin Denny, Alexander D MacKerell
Accurate and rapid estimation of relative binding affinities of ligand-protein complexes is a requirement of computational methods for their effective use in rational ligand design. Of the approaches commonly used, free energy perturbation (FEP) methods are considered one of the most accurate, although they require significant computational resources. Accordingly, it is desirable to have alternative methods of similar accuracy but greater computational efficiency to facilitate ligand design. In the present study relative free energies of binding are estimated for one or two non-hydrogen atom changes in compounds targeting the proteins ACK1 and p38 MAP kinase using three methods...
October 26, 2016: Journal of Computational Chemistry
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