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immunohistochemistry parotid

Lora H Rigatti, Tuna Toptan, Joseph T Newsome, Patrick S Moore, Yuan Chang
Polyomaviruses (PyVs) are known to infect a wide range of vertebrates and invertebrates and are associated with a broad spectrum of diseases, including cancers, particularly in immune-suppressed hosts. A novel polyomavirus, designated rat polyomavirus 2 (RatPyV2), was identified from a breeding colony of rats having X-linked severe combined immunodeficiency. Using a human panpolyomavirus immunohistochemistry test (P-PIT), RatPyV2 was initially detected in the parotid salivary gland of a colony member. Rolling circle amplification using DNA from harderian and parotid glands identified a novel 5...
November 2016: MSphere
Sainath K Andola, Meenakshi M Masgal, Rajeev M Reddy
Primary malignant lymphomas of the salivary glands are rare, accounting for 2-5% of salivary gland tumors and 5% of extranodal lymphomas, frequently seen in the parotid gland. There are single case reports mentioned in the literature. Clinical presentation is not characteristic and the disease is often overlooked with delay in diagnosis and treatment. We are reporting a case of bilateral parotid gland lymphoma in a 55-year-old male, presented with bilateral enlarged parotids. Magnetic resonance imaging (MRI) showed bilateral enlarged parotid glands with multiple well-defined intraparotid lesions...
October 2016: Journal of Cytology
Florin Liviu Gherghina, Adrian Camen, Maria Cristina Munteanu, Claudiu Mărgăritescu, Călin Gabriel Şarlă
Here we report the case of a 63-year-old female with a parotid sclerosing mucoepidermoid carcinoma diagnosed and treated at the Department of Oral and Maxillofacial Surgery, Emergency County Hospital of Craiova, Romania. The clinical and imaging investigation revealed a parotid malignant tumor with central fluid-filled cystic formation. Histopathology found an intermediate grade sclerosing mucoepidermoid carcinoma that invaded the adjacent adipose and striated muscle tissues, but without perineural and lymphovascular invasion...
2016: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
A C Liu, Y Chen, J S Jia, S Y Gao, Y Y Liu
IgG4-related disease is a systemic disorder involving a spectrum of multiple indications, and various histopathological features are shared among different IgG4-related disease subtypes, which challenge diagnosis, although certain syndromes have organ-specific involvement. Among them, Mikulicz's disease affecting the salivary and lacrimal glands, distinguished by often elevated levels of serum IgG4, infiltration of IgG4(+) plasma cells into target tissues, and diffuse swelling, mass formation, or fibrosis of affected organs...
December 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
Sonam Sharma, Rahul Mannan, Tejinder Bhasin
Intraparotid hemangioma of the children is a rare neoplasm, posing diagnostic dilemma to the diagnosticians as well as treating clinicians. A 2-month-old male infant presented with a diffuse swelling in the parotid region since birth that was gradually increasing in size. The ultrasonography (USG) report was suggestive of a right intraparotid mass of uncertain etiology; whereas magnetic resonance imaging (MRI) report inclined toward a mass associated with chronic inflammatory pathology. Fine-needle aspiration cytology (FNAC) suggested two differentials - a vascular neoplasm of the parotid gland and a spindle cell neoplasm with increased vascularity...
July 2016: Journal of Cytology
Alina Busch, Larissa Bauer, Eva Wardelmann, Claudia Rudack, Inga Grünewald, Markus Stenner
AIMS: Cancer of the major salivary glands comprises a morphologically diverse group of rare tumours of largely unknown cause. Epithelial-mesenchymal transition (EMT) has been shown to play a significant prognostic role in various human cancers. The aim was to assess the expression of EMT markers in different histological subtypes of parotid gland cancer (PGC) and analyse their prognostic value. METHODS: We examined 94 PGC samples (13 histological subtypes) for the expression of MIB-1, epithelial cadherin (E-cadherin), β-catenin, vimentin and cytokeratin 8/18 (CK8/18) by means of immunohistochemistry...
October 11, 2016: Journal of Clinical Pathology
Vitorino Modesto Dos Santos, Manoel da Costa Gondim Neto, Tereza Rodrigues de Carvalho Vieira de Melo, Iara Machado Motta
A 60-year Brazilian woman, presented with an enlarged lymph node in the neck for one year, and a superficial nonulcerated lesion was observed in the scalp. Fine needle aspiration and biopsy of the lymph node revealed amelanocytic metastasis, and immunohistochemistry study showed Melan-A/ Mart-1 antigen (clone A103 and S-100 protein). The entire suspected area of the scalp was further resected and an amelanotic melanoma without angiolymphatic invasion was diagnosed. Ultrasonography and PET-computed tomography showed hypermetabolic cystic area in the right parotid...
September 2016: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
Moacyr J B M Rêgo, Antônio F Silva Filho, Ana P V Sobral, Eduardo I C Beltrão
The formation of new and functional structural components of several organs, such as parotid glands, can be influenced by the glycocode. This study analyzed the glycobiology of parotid salivary gland tissue during fetal development using specific biochemical probes (lectins and antibodies). Eleven parotid gland samples from human fetuses were obtained from spontaneous abortions at 14-28 weeks of gestation, and tissue sections were analyzed for lectin histochemistry and immunohistochemistry. From the 18th to 26th week, Canavalia ensiformis agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin-I, peanut agglutinin, Sambucus nigra agglutinin, and Vicia villosa agglutinin lectin staining were predominantly observed in the apical and/or basement membranes of the ducts and tubulo-acinar units...
2016: Journal of Oral Science
Mariana Mirim Monteiro, Talyta Thereza Soares D'Epiro, Lisiane Bernardi, Anna Christina Medeiros Fossati, Marinilce Fagundes Dos Santos, Marcelo Lazzaron Lamers
OBJECTIVE: In this study we performed a temporal analysis of the effects of Diabetes Mellitus on morphology and laminin deposition in salivary glands of young (2 months-old) and aging (12 months-old) male Wistar rats, using immunohistochemistry. MATERIALS AND METHODS: The animals were divided in control and diabetic (Streptozotocin induced) groups and euthanized after short and long-term diabetes induction. RESULTS: Short-term induction led to vacuolization of parotid acinar cells and increased laminin deposition in both animal ages...
January 2017: Archives of Oral Biology
Nathalia Carolina Fernandes Fagundes, Luanna Melo Pereira Fernandes, Ricardo Sousa de Oliveira Paraense, Paulo Mecenas Alves de Farias-Junior, Francisco Bruno Teixeira, Sergio Melo Alves-Junior, João de Jesus Viana Pinheiro, Maria Elena Crespo-López, Cristiane Socorro Ferraz Maia, Rafael Rodrigues Lima
This study investigates morphological and biochemistry effects of binge ethanol consumption in parotid (PG) and submandibular (SG) salivary glands of rats from adolescence to adulthood. Female Wistar rats (n = 26) received ethanol at 3 g/kg/day (20% w/v) for 3 consecutive days/week from the 35th until the 62nd day of life. Animals were treated in two periods: 1 week (G1) and 4 weeks (G2), with a control (treated with distilled water) and an ethanol group to each period. In morphological analysis, morphometric and immunohistochemistry evaluation for smooth muscle actin (αSMA), cytokeratin-18 (CK-18), and vimentin (VIM) were made...
2016: Oxidative Medicine and Cellular Longevity
Sarah T Garber, Laith Khoury, Diana Bell, Donald F Schomer, Filip Janku, Ian E McCutcheon
BACKGROUND: Intracranial spread of an adenoid cystic carcinoma (ACC) of the parotid gland is rare, and metastatic ACC to the splenium of the corpus callosum mimicking butterfly glioblastoma (GBM) has not been reported previously. We report a rare case of metastasis to the splenium of the corpus callosum from ACC of the parotid gland. CASE DESCRIPTION: The tumor occupied the splenium and mimicked the presentation of a butterfly glioma. The patient had undergone parotidectomy 5 years before presentation with this intracranial lesion...
November 2016: World Neurosurgery
Vanessa Schneider, Antoine Nobile, Bertrand Duvoisin, Chiara Saglietti, Massimo Bongiovanni
Myoepithelioma is a rare and usually benign salivary gland tumor derived from myoepithelial cells. Variability in cellular morphology and stromal composition leads to diagnostic pitfalls on fine-needle aspiration cytology; therefore, histology and immunohistochemistry are often required for definitive diagnosis. We describe a case of parotid gland myoepithelioma in a 76-year-old woman, which was discovered incidentally on cerebral magnetic resonance imaging. Fine-needle aspiration sampled cohesive aggregates of spindle-shaped cells embedded in a fibrillary matrix as well as abundant mature adipocytes, initially considered as part of normal salivary gland parenchyma...
December 2016: Diagnostic Cytopathology
Dhanalakshmi Arumugam, Pavithra Thandavarayan, Lalitha Chidambaram, Sudha Boj, Sumathi Marudasalam
Yolk sac tumour also known as primitive endodermal tumour is the most common malignant germ cell tumour (GCT) in the paediatric age group. Most common sites of involvement are ovaries and testes, but rarely can occur in the extragonadal sites. In the head and neck region, yolk sac tumours have been reported in the nasopharynx, sinonasal tract, orbit, ear and parotid gland. Nasopharynx is an uncommon site for yolk sac tumour and very few cases of nasopharngeal pure yolk sac tumour have been reported so far. Yolk sac tumours are highly malignant and have a poor prognosis...
May 2016: Journal of Clinical and Diagnostic Research: JCDR
Bin Xu, Lu Wang, Laetitia Borsu, Ronald Ghossein, Nora Katabi, Ian Ganly, Snjezana Dogan
AIMS: In the current study, we aimed to examine primary parotid squamous cell carcinoma (ParSCC) for the presence of high-risk human papillomavirus (HR-HPV) and associated molecular alterations. METHODS AND RESULTS: Eight cases of ParSCC were retrieved after a detailed clinicopathological review to exclude the possibility of metastasis and/or extension from another primary site. HR-HPV status was determined on the basis of immunohistochemistry (IHC) for p16 expression and chromogenic in-situ hybridization (CISH) for HR-HPV...
December 2016: Histopathology
Tewarat Kumchantuek, Hiroki Nakata, Natthiya Sakulsak, Miyuki Yamamoto, Shoichi Iseki
OBJECTIVES: Calpains comprise a family of intracellular Ca(2+)-dependent cysteine proteases and are considered to play roles in various physiological phenomena with limited proteolytic activities against specific substrates. We herein revealed the expression and localization of calpain 3, the muscle-type calpain, in the submandibular gland (SMG) of mice. DESIGN: The expression of the mRNA for conventional, ubiquitous calpains 1 and 2 and skeletal muscle-specific calpain 3 was examined in the major salivary glands of mice using RT-PCR, and the expression and localization of calpain 3 protein was examined in the SMG of mice using immunohistochemistry and Western blotting...
October 2016: Archives of Oral Biology
Abhishek Purkayastha, Neelam Sharma, Abhishek Pathak, Bhupendra Nath Kapur, Vibha Dutta
Retinoblastoma (Rb) is a common childhood malignancy but bilateral Rb with metastasis to parotids is very uncommon. To the best of our knowledge, bilateral Rb metastasizing to parotids is very rare and this is the fifth such case reported in world literature till date in a 2-year-old male child who underwent exenteration of left eye for bilateral Rb and later developed recurrent metastasis to left parotid requiring parotidectomy. A year later he presented again with swelling left parotid region extending from occipital region reaching upto left anterior chest wall with intra-cranial extension on magnetic resonance imaging...
April 2016: Translational pediatrics
Jeannette P Staheli, Michael R Dyen, Gail H Deutsch, Ryan S Basom, Matthew P Fitzgibbon, Patrick Lewis, Serge Barcy
UNLABELLED: Human herpesvirus 6A (HHV-6A), HHV-6B, and HHV-7 are classified as roseoloviruses and are highly prevalent in the human population. Roseolovirus reactivation in an immunocompromised host can cause severe pathologies. While the pathogenic potential of HHV-7 is unclear, it can reactivate HHV-6 from latency and thus contributes to severe pathological conditions associated with HHV-6. Because of the ubiquitous nature of roseoloviruses, their roles in such interactions and the resulting pathological consequences have been difficult to study...
August 1, 2016: Journal of Virology
Francesco di Dio, Ilaria Mariotti, Elena Coccolini, Patrizia Bruzzi, Barbara Predieri, Lorenzo Iughetti
BACKGROUND: Rosai-Dorfman disease (RDD) is a rare form of histiocytosis characterized by histiocyte proliferation within lymph nodes and extranodal tissue. Here we report an unusual presentation of RDD in an Italian toddler. Moreover, we reviewed the pediatric case reports published between 2004 and 2014, focusing in particular on medical therapy. CASE PRESENTATION: We report the case of a 14-month-old child who developed a progressive swelling of the right parotid, associated with systemic symptoms and abnormal blood tests...
2016: BMC Pediatrics
Alok R Khandelwal, Xiaohui Ma, Paige Egan, Nadine M Kaskas, Tara Moore-Medlin, Gloria Caldito, Fleurette Abreo, Xin Gu, Lurie Aubrey, Edward Milligan, Cherie-Ann O Nathan
OBJECTIVE: Aggressive cutaneous squamous cell carcinoma (cSCC) patients are at increased risk of metastasis. Currently, there are no accepted criteria or biomarkers for reliably predicting individuals at risk for recurrence and metastasis. Our objective is to determine if pS6 and pERK can predict cSCC aggressiveness and to identify primary tumor characteristics that may predict parotid metastasis. STUDY DESIGN: Retrospective case series. SETTINGS: Tertiary care center...
August 2016: Otolaryngology—Head and Neck Surgery
Rachel L Geller, Kim Hookim, Harold C Sullivan, Lauren N Stuart, Mark A Edgar, Michelle D Reid
BACKGROUND: This study describes the cytologic features of 26 angiosarcomas diagnosed on fine-needle aspiration. METHODS: Twenty-six angiosarcomas from 20 patients were confirmed by cytomorphology and immunocytochemical (immunohistochemistry) positivity for at least 2 of 3 vascular markers. Specimens were examined for spindled/epithelioid/plasmacytoid single cells, 3-dimensional clusters, multiple prominent/bar-shaped nucleoli (5 times longer than their width), chromatin strands, abnormal mitoses, necrosis, and vasoformative features...
September 2016: Cancer Cytopathology
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