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https://www.readbyqxmd.com/read/29458137/bisphenol-a-alters-oocyte-maturation-by-prematurely-closing-gap-junctions-in-the-cumulus-cell-oocyte-complex
#1
Deyanira Guadalupe Acuña-Hernández, Laura Arreola-Mendoza, Ramsés Santacruz-Márquez, Sihomara Patricia García-Zepeda, Lyda Yuliana Parra-Forero, Jesús Alberto Olivares-Reyes, Isabel Hernández-Ochoa
In ovarian follicles, cumulus cells communicate with the oocyte through gap junction intercellular communication (GJIC), to nurture the oocyte and control its meiosis arrest and division. Bisphenol A (BPA) is a monomer found in polycarbonate-made containers that can induce functional alterations, including impaired oocyte meiotic division and reduced molecule transfer in GJIC. However, how BPA alters oocyte meiotic division is unclear. We investigated whether BPA effects on oocyte meiotic division were correlated with reduced transfer in GJIC...
February 16, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29454297/intracytoplasmic-sperm-injection-icsi-of-in-vitro-matured-oocytes-with-stored-epididymal-spermatozoa-in-camel-camelus-dromedarius-effect-of-exogenous-activation-on-in-vitro-embryo-development
#2
Nisar A Wani, Seungbum Hong
Experiments were conducted to investigate the development of in vitro matured camel oocytes after their intra-cytoplasmic sperm injection (ICSI) with epididymal sperm collected from slaughtered male camels. Ovaries and testes were collected from a local slaughterhouse in normal saline solution (NSS) at 37 °C and on ice (0-1 °C), respectively. Cumulus-oocyte complexes (COCs) aspirated from the follicles were randomly distributed to 4-well culture plates (20-25 COCs/well) containing 500 μL of maturation medium and cultured at 38...
February 8, 2018: Theriogenology
https://www.readbyqxmd.com/read/29448974/phyto-oestrogens-affect-fertilisation-and-embryo-development-in-vitro-in-sheep
#3
Anna Aryani Amir, Jennifer M Kelly, David O Kleemann, Zoey Durmic, Dominique Blache, Graeme B Martin
Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Each isoflavone compound was evaluated at five concentrations (0, 2.5, 5, 10, 25µgmL-1) and the entire protocol was repeated four times. Cumulus-oocyte complexes were randomly allocated to the treatments, then fertilised and cultured in vitro...
February 16, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29448847/effect-of-early-cumulus-cells-removal-and-early-rescue-icsi-on-pregnancy-outcomes-in-high-risk-patients-of-fertilization-failure
#4
Yuxia He, Haiying Liu, Haiyan Zheng, Li Li, Xin Fu, Jianqiao Liu
OBJECTIVE: The objective of this retrospective study was to evaluate clinical outcomes of early cumulus cells removal and early rescue intracytoplasmic sperm injection (ICSI) in high-risk patients of fertilization failure during human in vitro fertilization (IVF). METHODS: A total of 5,518 patients were enrolled between January 2014 to December 2016. Of which 505 couples performed short insemination with >30% fertilization rate were included in short-term IVF group, 102 patients detected total fertilization failure (TFF) were treated with early rescue ICSI (R-ICSI group), and 4911 couples underwent conventional IVF with overnight co-incubation of gametes (traditional IVF group)...
February 15, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29442425/effect-of-co-culture-human-endothelial-progenitor-cells-with-porcine-oocytes-during-maturation-subsequent-embryo-development-of-parthenotes-in-vitro
#5
Seok Hee Lee, Hyun Ju Oh, Min Jung Kim, Erif Maha Nugraha Setyawan, Yoo Bin Choi, Byeong Chun Lee
Human endothelial progenitor cells (EPCs) have been applied to regenerative medicine for their roles in angiogenesis as well as neovascularization, and these angiogenetic functions have beneficial effects on maturation of ovarian follicles. However, little information is available on whether EPCs on culture systems affect oocyte maturation and subsequent embryo development. Therefore, the objective of this study was to investigate the effect of EPC co-culture on porcine oocytes during in vitro maturation (IVM) and subsequent embryo development, and to examine gene expression in cumulus cells, oocytes and blastocysts...
February 14, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29435922/isolation-and-analysis-of-exosomal-micrornas-from-ovarian-follicular-fluid
#6
Juliano Da Silveira, Gabriella M Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A Winger, Gerrit J Bouma
Mammalian ovarian follicular growth is characterized by development of a large fluid filled antrum that separates mural granulosa cells and cumulus cells. Extensive communication between the different cell types is necessary for maturation of a developmentally competent oocyte. Here, we describe an approach for the isolation of cell-secreted exosomes from ovarian follicular fluid, identification of small RNAs (i.e., microRNAs) in exosomes, labeling of exosomes, and examining cell uptake of exosomes by follicular cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29428846/effect-of-different-shipping-temperatures-%C3%A2-22%C3%A2-%C3%A2-c-vs-%C3%A2-7%C3%A2-%C3%A2-c-and-holding-media-on-blastocyst-development-after-overnight-holding-of-immature-equine-cumulus-oocyte-complexes
#7
Mouhamadou Diaw, Renato M Salgado, Heloísa S Canesin, Nell Gridley, Katrin Hinrichs
Intracytoplasmic sperm injection (ICSI) is an important tool for equine embryo production in both clinical and research settings. In clinical ICSI programs, immature equine cumulus-oocyte complexes (COCs) are often collected at the mare's location and shipped to the ICSI laboratory. To simplify shipment and aid scheduling of subsequent procedures, COCs can be held overnight at room temperature (∼22 °C) before placement into maturation culture, with no detrimental effect on meiotic or developmental competence...
February 2, 2018: Theriogenology
https://www.readbyqxmd.com/read/29428720/effects-of-pre-incubation-with-c-type-natriuretic-peptide-on-nuclear-maturation-mitochondrial-behavior-and-developmental-competence-of-sheep-oocytes
#8
Tong Zhang, Xiaomei Fan, Ruilan Li, Chunqiang Zhang, Jiaxin Zhang
In vitro produced mammalian embryos suffer from developmental failure, with a large proportion showing embryonic retardation, degradation, or apoptosis. This failure is due, in part, to incomplete oocyte cytoplasmic maturation. C-type natriuretic peptide (CNP) has been reported to act as a meiotic inhibitor. Here we explored the potential effects of CNP pre-treatment sheep oocytes on nuclear maturation, changes in mitochondrial behavior and developmental competence of in vitro fertilized embryos. Sheep cumulus-oocyte complexes (COCs) were aspirated from abattoir-derived ovaries...
February 8, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29428306/cumulus-cell-transcriptome-profiling-is-not-predictive-of-live-birth-after-in%C3%A2-vitro-fertilization-a-paired-analysis-of-euploid-sibling-blastocysts
#9
Katherine A Green, Jason M Franasiak, Marie D Werner, Xin Tao, Jessica N Landis, Richard T Scott, Nathan R Treff
OBJECTIVE: To compare the transcriptome of cumulus cells associated with a euploid embryo that resulted in live birth with that of a sibling euploid embryo without sustained implantation. DESIGN: Paired analysis. SETTING: Academic institution. PATIENT(S): Couples undergoing IVF/intracytoplasmic sperm injection with preimplantation genetic screening with female age ≤42 years and normal ovarian reserve. INTERVENTION(S): Transcriptome profiling of cumulus cells from sibling oocytes for correlation with live birth after euploid blastocyst transfer...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29420611/inductions-of-granulosa-cell-luteinization-and-cumulus-expansion-are-dependent-on-the-fibronectin-integrin-pathway-during-ovulation-process-in-mice
#10
Hiroya Kitasaka, Tomoko Kawai, S A Masudul Hoque, Takashi Umehara, Youko Fujita, Masayuki Shimada
It has been known that EGF-like factor secreted from LH-stimulated granuloma cells acts on granulosa cells and cumulus cells to induce ovulation process. Granulosa cells are changed the morphology with differentiating cell functions to produce progesterone. Cumulus cells are detached to make a space between the cells to accumulate hyaluronan rich matrix. LH also changes extracellular matrix (ECM) components including fibronectin in the follicular walls and granulosa cell layers. EGF like factor and fibronectin synergistically play important roles in numerous cell functions, especially cancer cell migration, estimating that fibronectin would impact on granulosa cells and cumulus cells...
2018: PloS One
https://www.readbyqxmd.com/read/29408277/molecular-organization-and-mechanical-properties-of-the-hyaluronan-matrix-surrounding-the-mammalian-oocyte
#11
REVIEW
Antonietta Salustri, Luisa Campagnolo, Francesca Gioia Klinger, Antonella Camaioni
Successful ovulation and oocyte fertilization are essential prerequisites for the beginning of life in sexually reproducing animals. In mammalian fertilization, the relevance of the protein coat surrounding the oocyte plasma membrane, known as zona pellucida, has been widely recognized, while, until not too long ago, the general belief was that the cumulus oophorus, consisting of follicle cells embedded in a hyaluronan rich extracellular matrix, was not essential. This opinion was based on in vitro fertilization procedures, in which a large number of sperms are normally utilized and the oocyte can be fertilized even if depleted of cumulus cells...
February 2, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29402289/a-comparison-of-five-methods-of-measuring-mammographic-density-a-case-control-study
#12
Susan M Astley, Elaine F Harkness, Jamie C Sergeant, Jane Warwick, Paula Stavrinos, Ruth Warren, Mary Wilson, Ursula Beetles, Soujanya Gadde, Yit Lim, Anil Jain, Sara Bundred, Nicola Barr, Valerie Reece, Adam R Brentnall, Jack Cuzick, Tony Howell, D Gareth Evans
BACKGROUND: High mammographic density is associated with both risk of cancers being missed at mammography, and increased risk of developing breast cancer. Stratification of breast cancer prevention and screening requires mammographic density measures predictive of cancer. This study compares five mammographic density measures to determine the association with subsequent diagnosis of breast cancer and the presence of breast cancer at screening. METHODS: Women participating in the "Predicting Risk Of Cancer At Screening" (PROCAS) study, a study of cancer risk, completed questionnaires to provide personal information to enable computation of the Tyrer-Cuzick risk score...
February 5, 2018: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29396444/differential-long-non-coding-rna-expression-profiles-in-human-oocytes-and-cumulus-cells
#13
Julien Bouckenheimer, Patricia Fauque, Charles-Henri Lecellier, Céline Bruno, Thérèse Commes, Jean-Marc Lemaître, John De Vos, Said Assou
Progress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1)...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29395327/cloning-of-macaque-monkeys-by-somatic-cell-nuclear-transfer
#14
Zhen Liu, Yijun Cai, Yan Wang, Yanhong Nie, Chenchen Zhang, Yuting Xu, Xiaotong Zhang, Yong Lu, Zhanyang Wang, Muming Poo, Qiang Sun
Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys (Macaca fascicularis) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted SCNT embryos in surrogate monkeys...
January 20, 2018: Cell
https://www.readbyqxmd.com/read/29390119/metaphase-ii-oocytes-from-human-unilaminar-follicles-grown-in-a-multi-step-culture-system
#15
M McLaughlin, D F Albertini, W H B Wallace, R A Anderson, E E Telfer
STUDY QUESTION: Can complete oocyte development be achieved from human ovarian tissue containing primordial/unilaminar follicles and grown in vitro in a multi-step culture to meiotic maturation demonstrated by the formation of polar bodies and a Metaphase II spindle? SUMMARY ANSWER: Development of human oocytes from primordial/unilaminar stages to resumption of meiosis (Metaphase II) and emission of a polar body was achieved within a serum free multi-step culture system...
January 30, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29385411/palmitic-acid-induces-ceramide-accumulation-mitochondrial-protein-hyper-acetylation-and-mitochondrial-dysfunction-in-porcine-oocytes
#16
Nobuhiko Itami, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata
Low oocyte quality is a possible causal factor of obesity-induced infertility. High palmitic acid (PA) concentration in follicular fluid is a crucial feature noted in obese women. The present study examined how high PA concentration reduced mitochondrial quality in oocytes and investigated a possible countermeasure against mitochondrial dysfunction. Cumulus cell-oocyte complexes (COCs) were obtained from the ovaries of gilts, and incubated in medium containing PA (0.5 mM) or vehicle (BSA) for 44 h. Culturing oocytes at high PA concentration induced mitochondrial dysfunction determined by high ROS and low ATP content in oocytes...
January 29, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29378439/steroid-depleted-polycystic-ovarian-syndrome-serum-promotes-in-vitro-oocyte-maturation-and-embryo-development
#17
Elham Eyvaznejad, Mohammad Nouri, Aliyeh Ghasemzadeh, Amir Mehdizadeh, Vahideh Shahnazi, Samira Asghari, Alireza Mardomi, Masoud Darabi
In vitro maturation (IVM) of immature oocytes obtained from patients with polycystic ovarian syndrome (PCOS) is considered as a novel strategy in order to reduce clinical side effects and cost of in vitro fertilization (IVF) technique. The aim of this study was to evaluate the effects of PCOS whole and steroid-depleted serums on in vitro oocyte maturation indices. Patients with PCOS were selected according to the Rotterdam criteria. Cumulus-oocyte complexes and blood serums were collected and pooled. Cumulus cells and immature oocytes were treated with 10% whole or steroid-depleted serums...
January 29, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29375042/effect-of-bone-morphogenetic-protein-15-on-gonadotropin-stimulated-synthesis-of-hyaluronan-and-progesterone-in-porcine-ovarian-follicle
#18
E Nagyova, L Nemcova, A Bujnakova Mlynarcikova, M Blaha, R Prochazka, S Scsukova
Bone morphogenetic protein-15 (BMP-15), an oocyte-derived growth factor, has been shown to play integral roles in regulation of ovarian follicular function in mammals. Despite the recognition of the physiological importance of the BMP system in regulation of gonadotropin action in the ovary, molecular mechanisms of BMP-15 effect on oocyte and somatic follicular cell functions remain poorly understood. The objective of this study was to determine the effect of BMP-15 on the FSH/LH-stimulated synthesis of hyaluronan (HA) by oocyte cumulus complexes (OCC) and progesterone by OCC and granulosa cells (GC) in the presence or absence of serum using primary porcine cultures...
October 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29365049/reproductive-failure-in-mice-expressing-transgenic-follicle-stimulating-hormone-is-not-caused-by-loss-of-oocyte-quality
#19
Dannielle H Upton, Kirsty A Walters, Kirsten J McTavish, Janet Holt, David J Handelsman, Charles M Allan
Human female reproductive aging features declining ovarian follicle reserve and oocyte quality, and rising levels of circulating follicle stimulating hormone (FSH). We determined the effects of elevated FSH on oocyte-embryo development in mature mice exhibiting premature infertility caused by progressively rising transgenic human FSH (TgFSH) levels. Oocyte-embryo developmental competence and quality were examined using oocyte maturation and aneuploidy rates, biomarkers of oocyte quality, and reciprocal embryo transfers assessed for implantation and pregnancy...
January 22, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29361408/supplementing-in-vitro-embryo-production-media-by-nppc-and-sildenafil-affect-the-cytoplasmic-lipid-content-and-gene-expression-of-bovine-cumulus-oocyte-complexes-and-embryos
#20
Ramon Cesar Botigelli, Eduardo Montanari Razza, Elisa Mariano Pioltine, Patricia Kubo Fontes, Kátia Regina Lancellotti Schwarz, Cláudia Lima Verde Leal, Marcelo Fábio Gouveia Nogueira
In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We evaluated the effects on the lipid content (LC) of oocytes and embryos and also verified the expression of 96 transcripts related to competence in matured COCs and 96 transcripts related to embryo quality in blastocysts. After IVM, LC was decreased in oocytes by NPPC while sildenafil did not affect LC in oocytes...
January 17, 2018: Reproductive Biology
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