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Guang-Yu Bai, Si-Hang Song, Yu-Wei Zhang, Xiang Huang, Xing-Wei Huang, Rui-Zhen Sun, Lei Lei
Somatic cell nuclear transfer (SCNT) is an important technique for life science research. However, most SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we show that abnormal Xi occurs in somatic cell NT blastocysts, whereas in female blastocysts derived from cumulus cell nuclear transfer, both X chromosomes were inactive. H3K27me3 removal by Kdm6a mRNA overexpression could significantly improve preimplantation development of NT embryos, and even reached a 70.2% blastocyst rate of cleaved embryos compared with the 38...
December 14, 2017: Zygote: the Biology of Gametes and Early Embryos
A U Husna, A Azam, S Qadeer, M A Awan, S Nasreen, Q Shahzad, A Fouladi-Nashta, M Khalid, S Akhter
Routinely, swim-up method is used to separate high-quality sperm; however, long processing time and close cell-to-cell contact during the centrifugation step are inevitable elements of oxidative stress to sperm. The objective was to evaluate Sephadex™ and glass wool filtration to separate motile, intact and viable sperm for in vitro fertilization in buffalo. The cumulus-oocyte complexes (COCs) were collected from ovaries of slaughtered buffaloes by aspiration and matured for 24 hr in CO2 incubator at 38...
December 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
Fakhroddin Mesbah, Aris Donic Pracha, Tahereh Talaei-Khozani, Soghra Bahmanpour
Objective: The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods: In this experimental study, the COCs were incubated in Ham's F-10 supplemented with either 20% AOE, 20% fetal bovine serum (FBS) or serum-free media...
December 2017: Iranian Journal of Basic Medical Sciences
Jessica Vial, Sandrine Bony, Bjorn Stevens, Raphaela Vogel
Shallow cumulus clouds in the trade-wind regions are at the heart of the long standing uncertainty in climate sensitivity estimates. In current climate models, cloud feedbacks are strongly influenced by cloud-base cloud amount in the trades. Therefore, understanding the key factors controlling cloudiness near cloud-base in shallow convective regimes has emerged as an important topic of investigation. We review physical understanding of these key controlling factors and discuss the value of the different approaches that have been developed so far, based on global and high-resolution model experimentations and process-oriented analyses across a range of models and for observations...
2017: Surveys in Geophysics
Danielle Kaiser de Souza, Loise Pedrosa Salles, Ricardo Camargo, Laura Vanessa Mourão Gulart, Suellen Costa E Silva, Beatriz Dolabela de Lima, Fernando Araripe Gonçalves Torres, Alzira Amélia Martins Rosa E Silva
The purpose of this study was to evaluate the effects of FSH and PI3K on the nuclear maturation, viability, steroidogenesis and embryo development of bovine cumulus-oocyte complexes (COCs). Oocyte maturation was achieved with MIV B, MIV B+100 µM LY294002, MIV B+10 ng/mL follicle stimulating hormone (FSH), or MIV B+10 ng/mL FSH+100 µM LY294002 treatments for 22-24 h. After the cultured COCs were denuded, oocytes were separated into those that extruded polar bodies (mature) and those that did not, and real-time polymerase chain reaction (PCR) for BAX, BCL2, LHR, FSHR, CYP11A1, CYP19A1 and HSD17B1 genes was performed...
December 13, 2017: Zygote: the Biology of Gametes and Early Embryos
Mingzhu Xu, Yaqiong Zeng, Daming Chi, Linan Si, Xiao Qu, Juan Li
Lipid droplets (LDs) are the main energy resource for porcine preimplantation embryonic development. PLIN3 has been implicated in LD formation and regulation. Therefore, this study aimed to detect the dynamic pattern of PLIN3 in pig oocytes and cumulus cells (CC) during in vitro maturation (IVM), and to determine the relationship between PLIN3 and LD content. IVM with cumulus-enclosed oocytes (CEO), cumulus-denuded oocytes (DO) and the CCs denuded from the corresponding oocytes (DCC) was performed in porcine follicular fluid (PFF) or PFF-free optimized medium...
December 13, 2017: Zygote: the Biology of Gametes and Early Embryos
Sanghoon Lee, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon A Kim, Byeong Chun Lee
The beneficial effects of resveratrol on in vitro maturation (IVM) have been explained mainly by indirect antioxidant effects and limited information is available on the underlying mechanism by which resveratrol acts directly on porcine cumulus oocyte complexes (COCs). Recently, several studies reported that sonic hedgehog (SHH) signaling mediates resveratrol to exert its biological activities. Furthermore, SHH is an important signaling molecule for follicle development, oocyte maturation and embryo development...
December 7, 2017: Journal of Cellular Physiology
Shuang Liang, Zheng-Wen Nie, Minghui Zhao, Ying-Jie Niu, Kyung-Tae Shin, Xiang-Shun Cui
Excessive long-term fluoride intake is associated with several health problems, including infertility. However, limited information is available on the toxic effects of fluoride exposure on the female reproductive system, especially oocyte maturation. In this study, we investigated the toxic effect of sodium fluoride (NaF) exposure on porcine oocyte maturation and its possible underlying mechanisms. Our results showed that NaF exposure during porcine oocyte maturation inhibited cumulus cell expansion and impaired polar body extrusion...
December 6, 2017: Scientific Reports
Akane Sato, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Asuka Hara, Khurchabiling Atchalalt, Naoko Sugane, Rika Fukumori, Yoshikazu Nagao
The maturation rate of canine oocytes during in vitro maturation (IVM) needs to be improved. The present study was designed to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the IVM of canine oocytes. Ovaries were obtained by ovariohysterectomy and were sliced to release cumulus-oocyte complexes (COCs). In Experiment 1, the effects of different concentrations of IGF-1 on the nuclear maturation of oocytes was investigated. The COCs were cultured in a modified medium (mTCM199) with IGF-1 (0, 0...
December 7, 2017: Journal of Reproduction and Development
Hongmei Hu, Xianhong Mo, Xue Li, Xiangwei Fu, Yunpeng Hou
Intracellular free calcium ([Ca2+ ]i ) is essential for oocyte maturation and early embryonic development. Here, we investigated the role of [Ca2+ ]i in oocytes from cumulus-oocyte complexes (COCs) with respect to maturation and early embryonic development, using the calcium-buffering agent BAPTA-AM (1,2-bis[2-aminophenoxy]ethane-N,N,N',N'-tetraacetic acid tetrakis [acetoxymethyl ester]). COCs were graded based on compactness of the cumulus mass and appearance of the cytoplasm, with Grade 1 indicating higher quality and developmental potential than Grade 3...
December 4, 2017: Molecular Reproduction and Development
Nafisa Zohora, Ahmad Esmaielzadeh Kandjani, Antony Orth, Hannah M Brown, Mark R Hutchinson, Brant C Gibson
Conventional organic fluorophores lose their ability to fluoresce after repeated exposure to excitation light due to photobleaching. Therefore, research into emerging bright and photostable nanomaterials has become of great interest for a range of applications such as bio-imaging and tracking. Among these emerging fluorophores, metal oxide-based nanomaterials have attracted significant attention as a potential multifunctional material with photocatalytic and angeogenisis abilities in addition to fluorescnce applications...
December 4, 2017: Scientific Reports
Ayse Seyhan, Bulent Urman, Engin Turkgeldi, Baris Ata
Whether endometriomas grow because of supraphysiological oestradiol levels attained during ovarian stimulation for assisted reproduction techniques is a concern. In this prospective study, 25 women with 28 endometriomas underwent three-dimensional ultrasound using sono-automated volume calculation software. Endometrioma volume was measured on the first day of gonadotrophin injection (V1) and the day of ovulation trigger (V2). Nine (36%) women were stimulated in a gonadotrophin releasing hormone antagonist protocol (GnRH), 13 (52%) in a long, and three (12%) in an ultra-long GnRH agonist protocol...
November 17, 2017: Reproductive Biomedicine Online
Claudinéia Silva Mendes, Larissa Pires Barbosa, Monna Lopes de Araújo, Emmanuel Emydio Gomes Pinheiro, Maicon Pereira Lents, Lincoln da Silva Amorim, Cláudia Kazumi Kiya, Ana Lúcia Almeida Santana
In this study, goats were subjected to ovarian stimulation protocols to evaluate possible differences in ovarian follicular responses and oocyte production. Two experiments were conducted to assess the effects of hormonal protocol duration (seven or twelve days) and number of follicle stimulating hormone (FSH) applications (one or five doses). All animals received intravaginal sponges saturated with 60 mg medroxyprogesterone acetate and an application of 125 μg cloprostenol 72 h before the sponges were removed...
November 26, 2017: Theriogenology
Farzaneh Fesahat, Mohammad Hasan Sheikhha, Seyed Mehdi Kalantar, Nasim Tabibnejad, Razieh Dehghani Firouzabadi, Hojjatollah Saeedi, Mohammad Ali Khalili
The purpose was to assess the developmental competence of the in vitro or in vivo matured human oocytes as well as the apoptotic genes expression of cumulus cells (CCs) regarding nuclear maturity status of associated oocytes retrieved from stimulated ICSI cycles. A total of 590 oocytes and the associated CCs were retrieved and divided into groups of test and control according to the nuclear maturity status in order to the developmental evaluation as well as expression patterns of apoptosis-related genes using real time PCR...
November 28, 2017: Reproductive Biology
Minghui Yang, Jingli Tao, Menglong Chai, Hao Wu, Jing Wang, Guangdong Li, Changjiu He, Lu Xie, Pengyun Ji, Yunping Dai, Liguo Yang, Guoshi Liu
The inferior oocytes (IOs), which are not suitable for embryo development, occupy roughly one-third or more of the collected immature bovine oocytes. The IOs are usually discarded from the in vitro bovine embryo production process. Improving the quality of the inferior oocytes (IOs) and make them available in in vitro embryo production would have important biological, as well as commercial, value. This study was designed to investigate whether melatonin could improve the quality of IOs and make them usable in the in vitro maturation (IVM) and subsequent (in vitro fertilization) IVF embryo development...
November 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jakub A Kochan, Emilie C B Desclos, Ruben Bosch, Luna Meister, Lianne E M Vriend, Haico V Attikum, Przemek M Krawczyk
Most proteins involved in the DNA double-strand break response (DSBR) accumulate at the damage sites, where they perform functions related to damage signaling, chromatin remodeling and repair. Over the last two decades, studying the accumulation of many DSBR proteins provided information about their functionality and underlying mechanisms of action. However, comparison and systemic interpretation of these data is challenging due to their scattered nature and differing experimental approaches. Here, we extracted, analyzed and compared the available results describing accumulation of 79 DSBR proteins at sites of DNA damage, which can be further explored using Cumulus (http://www...
November 22, 2017: Nucleic Acids Research
Kazuki Kansaku, Shun Takeo, Nobuhiko Itami, Airi Kin, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata
Mitochondrial quality control is important for maintaining cellular and oocyte viability. In addition, aging affects mitochondrial quality in many cell types. In the present study, we examined how aging affects oocyte mitochondrial biogenesis and degeneration in response to induced mitochondrial dysfunction. Cumulus oocyte complexes were harvested from the ovaries of young (21‒45 months) and aged (≥120 months) cows and treated for 2 hours with 10 μM carbonyl cyanide-m- chlorophenylhydrazone (CCCP), or a vehicle control, after which cumulus oocyte complexes were subjected to in vitro fertilization and culture...
2017: PloS One
Virginia L Trasorras, María Ignacia Carretero, Deborah M Neild, Maria Graciela Chaves, Susana M Giuliano, Marcelo H Miragaya
The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection...
2017: Frontiers in Veterinary Science
Priscila Chediek Dall'Acqua, Beatriz Caetano da Silva Leão, Nathália Alves de Souza Rocha-Frigoni, Fernanda Patrícia Gottardi, Gisele Zoccal Mingoti
This study examined the effects of meiosis inhibition during bovine oocyte transportation on developmental competence and quality of produced embryos. The transportation medium was supplemented with: 100 μM butyrolactone I (BL), 500 μM IBMX + 100 μM forskolin (mSPOM), 100 μM milrinone (MR) or follicular fluid (bFF), and was carried out in a portable incubator for 6 h. Next, oocytes were in vitro matured (IVM) for 18 h, without the meiotic inhibitors, with the exception of mSPOM group, in which was added 20 μM cilostamide...
November 27, 2017: Zygote: the Biology of Gametes and Early Embryos
J A Ahmed, N Nashiruddullah, D Dutta, R K Biswas, P Borah
Cumulus-oocyte complexes (COCs) from cows were matured under normal (38.5°C) and elevated temperatures (41°C) simulating heat stress and their maturation was assessed based on measurement of cumulus expansion in both groups. There was a significant reduction (P<0.01) in maturation rate in the heat stressed oocytes. The ultrastructural events associated with in vitro oocyte maturation and changes associated with elevated temperature were also studied by transmission electron microscopy (TEM). Normal maturation cellular events were marked by migration of Golgi and mitochondria from the cortical regions, and conversely by a migration of cortical granules from the inner regions to a sub-perivitelline zone...
2017: Iranian Journal of Veterinary Research
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