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Thanik Chokjirawat, Mutchuporn Sukpresert, Wicharn Choktanasiri, Wanwisa Waiyaput, Duangporn Saengwimol, Aruchalean Taweewongsounton, Tanjitti Pongrujikorn, Chonthicha Satirapod
Aims: The aim is to study the relation and distribution in gene expression level of the luteinizing hormone receptor (LHR) gene and regulator of G-protein signaling 2 (RGS2) gene expression with oocyte maturation. Setting and Design: This cross-sectional study was undertaken in an instruction-based tertiary care infertility unit, department of obstetrics and gynecology. Materials and Methods: After controlled ovarian hyperstimulation, cumulus granulosa cells (CCs) from 59 oocytes among 18 women being treated by in vitro fertilization/intracytoplasmic sperm injection cycle technique from November 2015 to January 2016 were collected on the day of oocyte retrieval...
January 2018: Journal of Human Reproductive Sciences
Eduardo M Razza, Mateus J Sudano, Patricia K Fontes, Fernanda F Franchi, Katia Roberta A Belaz, Priscila H Santos, Anthony C S Castilho, Daniele F O Rocha, Marcos N Eberlin, Mariana F Machado, Marcelo F G Nogueira
Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus-oocyte complexes (COCs) on the lipid content of oocytes and blastocysts, on the membrane lipid composition of blastocysts and on the transcriptional profiling of cumulus cells and blastocysts in a high-throughput platform. Embryonic development rates to the morula (mean 56.1%) or blastocyst (mean 26...
April 23, 2018: Reproduction, Fertility, and Development
Olivia Moran, Tasnim Zaman, Andrea Eisen, Rochelle Demsky, Kristina Blackmore, Julia A Knight, Christine Elser, Ophira Ginsburg, Kevin Zbuk, Martin Yaffe, Steven A Narod, Leonardo Salmena, Joanne Kotsopoulos
PURPOSE: Mammographic density is a risk factor for breast cancer but the mechanism behind this association is unclear. The receptor activator of nuclear factor κB (RANK)/RANK ligand (RANKL) pathway has been implicated in the development of breast cancer. Given the role of RANK signaling in mammary epithelial cell proliferation, we hypothesized this pathway may also be associated with mammographic density. Osteoprotegerin (OPG), a decoy receptor for RANKL, is known to inhibit RANK signaling...
April 20, 2018: Cancer Causes & Control: CCC
Luiz Fernando Feres, Luiz Gustavo Bruno Siqueira, Miller Pereira Palhao, Lívia Loiola Dos Santos, Felipe Zandonadi Brandao, Joao Henrique Moreira Viana
The aim of the present study was to evaluate the likelihood of pregnancy of in vitro-produced (IVP) embryos from batches with distinct relative efficiencies. Data were retrospectively analyzed from 605 transvaginal ultrasonic-guided follicle aspiration sessions (OPU) followed by in vitro embryo production (IVEP) and 2456 fresh embryo transfers (ET), performed between 2008 and 2012 in individuals of the Gir (dairy Bos indicus) breed. The OPU and IVEP were performed using standard procedures by a single group of technicians at the same laboratory facility...
April 15, 2018: Animal Reproduction Science
X L Xu, J H Bai, T Feng, L L Xiao, Y Q Song, Y X Xiao, Y Liu
BACKGROUND: Studies have shown that ghrelin plays an important role in the mammalian reproductive system, including the central, gonadal levels, and also during in vitro maturation of oocytes; however, the functions of ghrelin in bovine oocyte meiosis require further investigation. OBJECTIVE: We aimed to evaluate the effects of an n-octanoylated ghrelin peptide on oocyte meiotic resumption and the developmental competence of mature oocytes in vitro. EXPERIMENTAL: design: The expression of GHRL(encoding ghrelin) mRNA and its receptor (the growth hormone secretagogue receptor, GHSR) in the cumulus-oocyte complex (COCs), denuded oocytes (DOs), and cumulus cells (CCs) was assessed using quantitative real-time reverse transcription PCR (qRT-PCR), and the effects of the n-octanoylated ghrelin peptide on meiotic resumption were studied at four different doses (0, 10, 50, and 100 ng/mL) in a 6 h culture system...
April 16, 2018: General and Comparative Endocrinology
Sanghoon Lee, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon A Kim, Byeong Chun Lee
Resveratrol and melatonin are known for their antioxidant properties and have various biological activities. The fact that they exhibit possible synergistic effects in phytomedicine researches suggests the use of a combination of these agents to promote porcine in vitro maturation (IVM) of oocytes. Therefore, we investigated the effects of resveratrol and/or melatonin on this process; cumulus-oocyte complexes underwent IVM culture with four different conditions (control, resveratrol, melatonin or their combination)...
April 5, 2018: Theriogenology
B R Abdel-Halim
The current study was conducted to investigate the effect of linoleic acid-treated oocytes during in vitro maturation (IVM) on cumulus cell expansion, oocyte nuclear maturation, blastocyst development and DNA damage of cumulus cells and how its effects were alleviated by supplementation of the maturation medium with Chitosan nanoparticles (CSNPs). Inhibitory effects of LA at a concentration (100 μM/ml) or more significantly decreased the percentage of oocytes reaching metaphase II stage, the rate of fully expanded cumulus cells 24 h after IVM and the percentage of blastocyst rate compared with the control (p < 0...
March 31, 2018: Theriogenology
Kazuki Kansaku, Yasuhisa Munakata, Nobuhiko Itami, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata
This study examined the concentration of cell-free mitochondrial DNA (cf-mtDNA) in porcine follicular fluid (FF) and explored whether the cfDNA level in the culture medium could reflect mitochondrial dysfunction in cumulus cell-oocyte complexes (COCs). cfDNA concentration was higher in the fluid of small-sized follicles, compared to that in larger follicles. The length of cfDNA ranged from short (152 bp) to long (1,914 bp) mtDNA in FF, detected by polymerase chain reaction (PCR). cfDNA concentration in FF significantly correlated with the mtDNA copy number in FF but not with the number of one-copy gene (nuclear DNA) in FF...
April 3, 2018: Journal of Reproduction and Development
Beatriz Fernandez-Fuertes, Alfonso Blanco-Fernandez, Colm J Reid, Kieran Meade, S Fair, Pat Lonergan
This study tested the hypothesis that sperm sialic acid (Sia) is required to reach the site of fertilization, and that successful fertilization requires recognition of Sia from both the sperm and oocyte to occur. In addition, it has recently been reported that Siglecs (Sia-binding-immunoglobulin-like lectins) are present on the sperm surface. Thus, the possibility that the recognition of oocyte Sia was sperm-Siglec-mediated was also addressed. Sperm exposed to neuraminidase (NMase) exhibited lower overall and progressive motility, which translated to a decreased ability to swim through cervical mucus from cows in oestrus...
April 4, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Irma Virant-Klun, Andrej Vogler
BACKGROUND: Some reports show that it is possible to isolate immature oocytes from human ovarian tissue retrieved by a cortex biopsy or ovariectomy of non-stimulated ovaries and mature them in vitro. The mature oocytes can be vitrified and stored for in vitro fertilization, which, along with ovarian tissue cryopreservation, is mostly practiced in young cancer patients to preserve their fertility. There is much less data on this new approach in women with a natural ovarian insufficiency, which can be caused by different factors, including viral infection...
April 5, 2018: Reproductive Biology and Endocrinology: RB&E
S C Wei, Y Y Deng, L J Lai, H Q Liang, G Z Lin, Z D Gong
The present study aimed to assess LH effects on in vitro maturation (IVM) and apoptosis and also to explore the gene expressions of LHR and FSHR in cumulus-oocyte complexes (COCs) of the sheep. COCs were in vitro matured 24h in the IVM medium supplemented with varying concentrations of LH (0, 5, 10, 20 and 30 μg/mL). They were allocated into LH-1 (control group), LH-2, LH-3, LH-4 and LH-5 groups, respectively. FSH (10 IU/mL) addition was as a positive control (FSH group). COCs apoptosis was assessed by TUNEL...
December 2017: Polish Journal of Veterinary Sciences
Caizhu Wang, Guixue Feng, Jinhui Shu, Hong Zhou, Bo Zhang, Huanhua Chen, Ruoyun Lin, Xianyou Gan, Zhulian Wu, Tinglv Wei
OBJECTIVE: To investigate the effectiveness of cumulus oophorus complexes (COCs) in the physiologic selection of spermatozoa for intracytoplasmic sperm injection (ICSI). DESIGN: A prospective sibling oocytes study. SETTING: Center of reproductive medicine. PATIENT(S): Couples undergoing ICSI during 2016, females aged ≤38 years, and at least six metaphase II (MII) oocytes retrieved. Sixty patients were included in the study...
March 28, 2018: Fertility and Sterility
JoAnne S Richards, Mario Ascoli
The central role of luteinizing hormone (LH) and its receptor (LHCGR) in triggering ovulation has been recognized for decades. Because the LHCGR is present in the mural (outermost) granulosa cell layer of preovulatory follicles (POFs), the LH-initiated signal has to be transmitted to another somatic cell type (cumulus granulosa cells) and the oocyte to release a fertilizable oocyte. Recent studies have shown that activation of the LHCGR initiates vectorial transfer of information among the two somatic cell types and the oocyte and the molecules and signaling pathways involved are now better understood...
March 27, 2018: Trends in Endocrinology and Metabolism: TEM
Lina Wei, Chunlei Xin, Wenjuan Wang, Cuifang Hao
PURPOSE: This study aimed to screen key genes and pathways involved in obese polycystic ovary syndrome (PCOS), and predict drugs for treatment of obese PCOS via bioinformatics approaches. METHODS: Microarray dataset GSE10946 were downloaded from the Gene Expression Omnibus database, including 7 cumulus cell samples from obese PCOS patients and 6 lean control samples. Differentially expressed genes (DEGs) between obese PCOS and controls were obtained using Bayesian test after data preprocessing, followed by functional enrichment analyses for DEGs...
March 27, 2018: Gene
Gustavo Nardini Cecchino, Emre Seli, Eduardo Leme Alves da Motta, Juan Antonio García-Velasco
Mitochondria have been implicated as key factors regulating female reproductive processes. Notable progress has been made in determining the role of mitochondria with respect to oocyte maturation, fertilization and early embryo development. In addition, mitochondrial function and dysfunction has been the subject of various studies in ovarian ageing and metabolic stress models. However, the overall mitochondrial impact on female fertility is yet to be uncovered. The mitochondrial DNA content of granulosa, cumulus and trophectoderm cells is being explored as a biomarker of oocyte quality and embryo viability...
March 8, 2018: Reproductive Biomedicine Online
Kouji Komatsu, Satoru Masubuchi
Proper development and maturation of oocytes requires interaction with granulosa cells. Previous reports have indicated that mammalian oocytes connect with cumulus cells through gap junctions at the tip of transzonal projections that extend from the cells. Although the gap junctions between oocytes and transzonal projections provide a pathway through which small molecules (< 1 kDa) can travel, it is unclear how molecules > 1 kDa are transported between the oocytes and cumulus cells. In this study, we presented new connections between oocytes and granulosa cells...
March 24, 2018: Biology of Reproduction
Chang Liu, Bao Yuan, Hongyan Chen, Mingqiang Xu, Xulei Sun, JiaJun Xu, Yan Gao, Chengzhen Chen, Hao Jiang, Jiabao Zhang
BACKGROUND/AIMS: Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), which are secreted by oocytes, are important regulators of follicular growth and development and ovarian function. These two factors can regulate the proliferation and apoptosis of cumulus cells via modulation of the Smad signaling pathway. Studies have shown that BMP15 and GDF9 can affect the level of miR-375, whereas the target gene of miR-375 is BMPR2, the type II receptor of BMP15 and GDF9...
March 21, 2018: Cellular Physiology and Biochemistry
J Ispada, T A Rodrigues, P H B Risolia, R S Lima, D R Gonçalves, D Rettori, M Nichi, W B Feitosa, F F Paula-Lopes
The cellular mechanisms induced by elevated temperature on oocytes are not fully understood. However, there is evidence that some of the deleterious effects of heat shock are mediated by a heat-induced increase in reactive oxygen species (ROS). In this context, carotenoid antioxidants might have a thermoprotective effect. Therefore, the objective of this study was to determine the role of astaxanthin (AST) on oocyte ROS production and on the redox profile and developmental competency of cumulus-oocyte complexes (COCs) after 14h heat shock (41°C) during in vitro maturation (IVM)...
March 28, 2018: Reproduction, Fertility, and Development
V Torres, M Hamdi, M G Millán de la Blanca, R Urrego, J Echeverri, A López-Herrera, D Rizos, A Gutiérrez-Adán, M J Sánchez-Calabuig
Antioxidants have been widely used during in vitro production to decrease the negative effect of reactive oxygen species. It was reported that the complex resveratrol-methyl β-cyclodextrin (RV-CD) improves resveratrol's stability and bioavailability and increases its antioxidant activity. This study evaluates the effect of RV-CD during in vitro oocyte maturation (IVM) or in vitro embryo culture (IVC) on developmental competence and quantitative changes in gene expression of developmental important genes. In experiment 1, RV-CD was added to IVM media and maturation level, embryo development and oocytes, cumulus cells, and blastocysts gene expression by RT-qPCR were examined...
March 26, 2018: Reproduction in Domestic Animals, Zuchthygiene
B R Silva, C Maside, L A Vieira, J Cadenas, B G Alves, A C A Ferreira, F L N Aguiar, A L C Silva, J R Figueiredo, J R V Silva
The aim of the present study was to evaluate the effect of frutalin (FTL) on in vitro maturation (IVM), and fertilization (IVF) of pig oocytes. In the Experiment 1, cumulus-oocyte complexes (COCs) were submitted to IVM in maturation medium alone or supplemented with different FTL concentration (0.6, 6 and 60 μg/mL), or 0.3 μg/mL doxorubicin (DXR). After IVM, some oocytes were evaluated for chromatin configuration, and the remaining oocytes were submitted to in vitro fertilization. In Experiment 2, matured oocytes were fertilized in IVF medium alone (control) or in presence of different FTL concentration (0...
March 17, 2018: Animal Reproduction Science
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