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https://www.readbyqxmd.com/read/27936405/identification-of-lipopolysaccharide-interacting-plasma-membrane-type-proteins-in-arabidopsis-thaliana
#1
Cornelius S Vilakazi, Ian A Dubery, Lizelle A Piater
Lipopolysaccharide (LPS) is an amphiphatic bacterial glycoconjugate found on the external membrane of Gram-negative bacteria. This endotoxin is considered as a microbe-associated molecular pattern (MAMP) molecule and has been shown to elicit defense responses in plants. Here, LPS-interacting proteins from Arabidopsis thaliana plasma membrane (PM)-type fractions were captured and identified in order to investigate those involved in LPS perception and linked to triggering of innate immune responses. A novel proteomics-based affinity-capture strategy coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed for the enrichment and identification of LPS-interacting proteins...
November 30, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27936346/antibodies-to-staphylococcus-aureus-capsular-polysaccharides-5-and-8-perform-similarly-in-vitro-but-are-functionally-distinct-in-vivo
#2
Bo Liu, Saeyoung Park, Christopher D Thompson, Xue Li, Jean C Lee
The capsular polysaccharide (CP) produced by Staphylococcus aureus is a virulence factor that allows the organism to evade uptake and killing by host neutrophils. Polyclonal antibodies to the serotype 5 (CP5) and type 8 (CP8) capsular polysaccharides are opsonic and protect mice against experimental bacteremia provoked by encapsulated staphylococci. Thus, passive immunotherapy using CP antibodies has been considered for the prevention or treatment of invasive antibiotic-resistant S. aureus infections. In this report, we generated monoclonal antibodies (mAbs) against S...
December 9, 2016: Virulence
https://www.readbyqxmd.com/read/27936230/the-circulating-ctrp13-in-type-2-diabetes-and-non-alcoholic-fatty-liver-patients
#3
Mehrnoosh Shanaki, Reza Fadaei, Nariman Moradi, Solaleh Emamgholipour, Hossein Poustchi
Numerous studies have shown that C1q/TNF-related proteins (CTRPs) are involved in the pathophysiology of metabolic disorders, such as Non-alcoholic fatty liver disease (NAFLD) and Type 2 Diabetes (T2DM). There is a little information concerning CTRP13 in the context of NAFLD and T2DM. We evaluated the plasma levels of CTRP13 in healthy control and patients with NAFLD, T2DM and NAFLD+T2DM, and also correlations between CTRP13 plasma levels and clinical and subclinical features. Circulating CTRP13 was examined in 88 male (20 healthy control, 22 T2DM patients, 22 NAFLD patients and 22 NAFLD+T2DM patients)...
2016: PloS One
https://www.readbyqxmd.com/read/27936199/circulating-microvesicles-are-elevated-acutely-following-major-burns-injury-and-associated-with-clinical-severity
#4
Kieran P O'Dea, John R Porter, Nikhil Tirlapur, Umar Katbeh, Suveer Singh, Jonathan M Handy, Masao Takata
Microvesicles are cell-derived signaling particles emerging as important mediators and biomarkers of systemic inflammation, but their production in severe burn injury patients has not been described. In this pilot investigation, we measured circulating microvesicle levels following severe burns, with severe sepsis patients as a comparator group. We hypothesized that levels of circulating vascular cell-derived microvesicles are elevated acutely following burns injury, mirroring clinical severity due to the early onset and prevalence of systemic inflammatory response syndrome (SIRS) in these patients...
2016: PloS One
https://www.readbyqxmd.com/read/27936190/two-isoforms-of-yersinia-pestis-plasminogen-activator-pla-intraspecies-distribution-intrinsic-disorder-propensity-and-contribution-to-virulence
#5
Svetlana V Dentovskaya, Mikhail E Platonov, Tat'yana E Svetoch, Pavel Kh Kopylov, Tat'yana I Kombarova, Sergey A Ivanov, Rima Z Shaikhutdinova, Lyubov' V Kolombet, Sadhana Chauhan, Vitaly G Ablamunits, Vladimir L Motin, Vladimir N Uversky, Andrey P Anisimov
It has been shown previously that several endemic Y. pestis isolates with limited virulence contained the I259 isoform of the outer membrane protease Pla, while the epidemic highly virulent strains possessed only the T259 Pla isoform. Our sequence analysis of the pla gene from 118 Y. pestis subsp. microtus strains revealed that the I259 isoform was present exclusively in the endemic strains providing a convictive evidence of more ancestral origin of this isoform. Analysis of the effects of the I259T polymorphism on the intrinsic disorder propensity of Pla revealed that the I259T mutation slightly increases the intrinsic disorder propensity of the C-terminal tail of Pla and makes this protein slightly more prone for disorder-based protein-protein interactions, suggesting that the T259 Pla could be functionally more active than the I259 Pla...
2016: PloS One
https://www.readbyqxmd.com/read/27936169/effects-of-supplementation-of-the-synbiotic-ecologic%C3%A2-825-fos-p6-on-intestinal-barrier-function-in-healthy-humans-a-randomized-controlled-trial
#6
E Wilms, J Gerritsen, H Smidt, I Besseling-van der Vaart, G T Rijkers, A R Garcia Fuentes, A A M Masclee, F J Troost
BACKGROUND AND AIMS: Probiotics, prebiotics and synbiotics have been suggested as dietary strategies to improve intestinal barrier function. This study aimed to assess the effect of two weeks synbiotic supplementation on intestinal permeability under basal and stressed conditions. Secondary aims were the assessment of two weeks synbiotic supplementation on systemic immune function and gastrointestinal symptoms including defecation pattern. DESIGN: Twenty healthy adults completed a double-blind, controlled, randomized, parallel design study...
2016: PloS One
https://www.readbyqxmd.com/read/27936141/hbs-binding-to-gp1b%C3%AE-activates-platelets-in-sickle-cell-disease
#7
Gowtham K Annarapu, Rashi Singhal, Avinash Gupta, Sheetal Chawla, Harish Batra, Tulika Seth, Prasenjit Guchhait
Intravascular hemolysis increases the risk of thrombosis in hemolytic disorders. Our previous study showed that the binding of adult hemoglobin (HbA) to glycoprotein (GP) 1bα induced the activation of platelets. The elevated plasma Hb or platelet surface bound Hb positively correlated with platelet activation in patients with paroxysmal nocturnal hemoglobinuria (PNH). Furthermore, this study shows that the sickle Hb [HbS, occurs due to single nucleotide polymorphism at A>T of β-globin gene of Hb and causes sickle cell disease (SCD)] also bound to GP1bα and activated platelets in a concentration-dependent manner...
2016: PloS One
https://www.readbyqxmd.com/read/27936130/angiogenic-proteins-placental-weight-and-perinatal-outcomes-among-pregnant-women-in-tanzania
#8
Chloe R McDonald, Anne M Darling, Enju Liu, Vanessa Tran, Ana Cabrera, Said Aboud, Willy Urassa, Kevin C Kain, Wafaie W Fawzi
INTRODUCTION: Placental vascular development, and ultimately placental weight, is essential to healthy fetal development. Here, we examined placental weight in a cohort of Tanzanian women in association with angiogenic proteins known to regulate placental vascular development and perinatal outcomes. METHODS: A total of n = 6579 women with recorded placental weight were included in this study. The relative risk of adverse perinatal outcomes (Apgar score, death, asphyxia, respiratory distress, seizures, pneumonia and sepsis) was compared between placental weight in the bottom and top 10th percentiles...
2016: PloS One
https://www.readbyqxmd.com/read/27936120/sweat-facilitated-amino-acid-losses-in-male-athletes-during-exercise-at-32-34%C3%A2-c
#9
R Hugh Dunstan, Diane L Sparkes, Benjamin J Dascombe, Margaret M Macdonald, Craig A Evans, Christopher J Stevens, Marcus J Crompton, Johan Gottfries, Jesse Franks, Grace Murphy, Ryan Wood, Timothy K Roberts
Sweat contains amino acids and electrolytes derived from plasma and athletes can lose 1-2L of sweat per hour during exercise. Sweat may also contain contributions of amino acids as well as urea, sodium and potassium from the natural moisturizing factors (NMF) produced in the stratum corneum. In preliminary experiments, one participant was tested on three separate occasions to compare sweat composition with surface water washings from the same area of skin to assess contributions from NMF. Two participants performed a 40 minute self-paced cycle session with sweat collected from cleansed skin at regular intervals to assess the contributions to the sweat load from NMF over the period of exercise...
2016: PloS One
https://www.readbyqxmd.com/read/27936119/anti-tnf-%C3%AE-drugs-differently-affect-the-tnf%C3%AE-stnfr-system-and-monocyte-subsets-in-patients-with-psoriasis
#10
Lara Gibellini, Sara De Biasi, Elena Bianchini, Regina Bartolomeo, Antonella Fabiano, Marco Manfredini, Federica Ferrari, Giuseppe Albertini, Tommaso Trenti, Milena Nasi, Marcello Pinti, Anna Iannone, Carlo Salvarani, Andrea Cossarizza, Giovanni Pellacani
TNF-α has a central role in the development and maintenance of psoriatic plaques, and its serum levels correlate with disease activity. Anti-TNF-α drugs are, however, ineffective in a relevant percentage of patients for reasons that are currently unknown. To understand whether the response to anti-TNF-α drugs is influenced by the production of anti-drug antibodies or by the modulation of the TNFα-TNFα receptor system, and to identify changes in monocyte phenotype and activity, we analysed 119 psoriatic patients who either responded or did not respond to different anti-TNF-α therapies (adalimumab, etanercept or infliximab), and measured plasma levels of TNF-α, TNF-α soluble receptors, drug and anti-drug antibodies...
2016: PloS One
https://www.readbyqxmd.com/read/27936114/long-circulating-curcumin-loaded-liposome-formulations-with-high-incorporation-efficiency-stability-and-anticancer-activity-towards-pancreatic-adenocarcinoma-cell-lines-in-vitro
#11
Mohamed Mahmud, Adriana Piwoni, Nina Filiczak, Martyna Janicka, Jerzy Gubernator
The incorporation of hydrophobic drugs into liposomes improve their bioavailability and leads to increased stability and anticancer activity, along with decreased drug toxicity. Curcumin (Cur) is a natural polyphenol compound with a potent anticancer activity in pancreatic adenocarcinoma (PA). In the present study, different types of Cur-loaded liposomal formulations were prepared and characterized in terms of size, shape, zeta potential, optimal drug-to-lipid ratio and stability at 4°C, 37°C; and in human plasma in vitro...
2016: PloS One
https://www.readbyqxmd.com/read/27936098/analysis-of-hiv-diversity-in-hiv-infected-black-men-who-have-sex-with-men-hptn-061
#12
Iris Chen, Gordon Chau, Jing Wang, William Clarke, Mark A Marzinke, Vanessa Cummings, Autumn Breaud, Oliver Laeyendecker, Sheldon D Fields, Sam Griffith, Hyman M Scott, Steven Shoptaw, Carlos Del Rio, Manya Magnus, Sharon Mannheimer, Hong-Van Tieu, Darrell P Wheeler, Kenneth H Mayer, Beryl A Koblin, Susan H Eshleman
BACKGROUND: HIV populations often diversify in response to selective pressures, such as the immune response and antiretroviral drug use. We analyzed HIV diversity in Black men who have sex with men who were enrolled in the HIV Prevention Trials Network 061 study. METHODS: A high resolution melting (HRM) diversity assay was used to measure diversity in six regions of the HIV genome: two in gag, one in pol, and three in env. HIV diversity was analyzed for 146 men who were HIV infected at study enrollment, including three with acute infection and 13 with recent infection (identified using a multi-assay algorithm), and for 21 men who seroconverted during the study...
2016: PloS One
https://www.readbyqxmd.com/read/27936074/association-between-metabolic-syndrome-and-cognitive-impairment-after-acute-ischemic-stroke-a-cross-sectional-study-in-a-chinese-population
#13
Pan Li, Wei Quan, Da Lu, Yan Wang, Hui-Hong Zhang, Shuai Liu, Rong-Cai Jiang, Yu-Ying Zhou
BACKGROUND AND OBJECTIVES: Metabolic syndrome (MetS), a risk factor for many vascular conditions, is associated with vascular cognitive disorders. The objective of the present study was to explore the associations of MetS and its individual components with the risks of cognitive impairment and neurological dysfunction in patients after acute stroke. METHODS: This cross-sectional study enrolled 840 patients ranging in age from 53 to 89 years from the Tianjin area of North China...
2016: PloS One
https://www.readbyqxmd.com/read/27936047/cross-neutralizing-antibodies-in-hiv-1-individuals-infected-by-subtypes-b-f1-c-or-the-b-bbr-variant-in-relation-to-the-genetics-and-biochemical-characteristics-of-the-env-gene
#14
Dalziza Victalina de Almeida, Karine Venegas Macieira, Beatriz Gilda Jegerhorn Grinsztejn, Valdiléa Gonçalves Veloso Dos Santos, Monick Lindenmeyer Guimarães
Various HIV-1 env genetic and biochemical features impact the elicitation of cross-reactive neutralizing antibodies in natural infections. Thus, we aimed to investigate cross-neutralizing antibodies in individuals infected with HIV-1 env subtypes B, F1, C or the B/Bbr variant as well as env characteristics. Therefore, plasma samples from Brazilian chronically HIV-1 infected individuals were submitted to the TZM-bl neutralization assay. We also analyzed putative N-glycosylation sites (PNGLs) and the size of gp120 variable domains in the context of HIV-1 subtypes prevalent in Brazil...
2016: PloS One
https://www.readbyqxmd.com/read/27936037/the-antioxidative-fraction-of-white-mulberry-induces-apoptosis-through-regulation-of-p53-and-nf%C3%AE%C2%BAb-in-eac-cells
#15
Ahm Khurshid Alam, Asm Sakhawat Hossain, Muhammad Ali Khan, Syed Rashel Kabir, Md Abu Reza, Md Mahbubur Rahman, Mohammad Saiful Islam, Md Aziz Abdur Rahman, Mamunur Rashid, Md Golam Sadik
In this study, the antioxidative fraction of white mulberry (Morus alba) was found to have an apotogenic effect on Ehrlich's ascites carcinoma cell-induced mice (EAC mice) that correlate with upregulated p53 and downregulated NFκB signaling. The antioxidant activities and polyphenolic contents of various mulberry fractions were evaluated by spectrophotometry and the ethyl acetate fraction (EAF) was selected for further analysis. Strikingly, the EAF caused 70.20% tumor growth inhibition with S-phase cell cycle arrest, normalized blood parameters including red/white blood cell counts and suppressed the tumor weight of EAC mice compared with untreated controls...
2016: PloS One
https://www.readbyqxmd.com/read/27935992/intense-exercise-and-aerobic-conditioning-associated-with-chromium-or-l-carnitine-supplementation-modified-the-fecal-microbiota-of-fillies
#16
Maria Luiza Mendes de Almeida, Walter Heinz Feringer, Júlia Ribeiro Garcia Carvalho, Isadora Mestriner Rodrigues, Lilian Rezende Jordão, Mayara Gonçalves Fonseca, Adalgiza Souza Carneiro de Rezende, Antonio de Queiroz Neto, J Scott Weese, Márcio Carvalho da Costa, Eliana Gertrudes de Macedo Lemos, Guilherme de Camargo Ferraz
Recent studies performed in humans and rats have reported that exercise can alter the intestinal microbiota. Athletic horses perform intense exercise regularly, but studies characterizing horse microbiome during aerobic conditioning programs are still limited. Evidence has indicated that this microbial community is involved in the metabolic homeostasis of the host. Research on ergogenic substances using new sequencing technologies have been limited to the intestinal microbiota and there is a considerable demand for scientific studies that verify the effectiveness of these supplements in horses...
2016: PloS One
https://www.readbyqxmd.com/read/27935988/effects-of-a-community-based-healthy-lifestyle-intervention-program-co-help-among-adults-with-prediabetes-in-a-developing-country-a-quasi-experimental-study
#17
Norliza Ibrahim, Foong Ming Moy, Intan Attikah Nur Awalludin, Zainudin Mohd Ali, Ikram Shah Ismail
BACKGROUND: The prevalence of type 2 diabetes among Malaysian adults has increased by more than two folds over the past two decades. Strategies to collaborate with the existing community partners may become a promising channel for wide-scale dissemination of diabetes prevention in the country. The objectives of this study were to determine the effects of community-based lifestyle interventions delivered to adults with prediabetes and their health-related quality of life as compared to the usual care group...
2016: PloS One
https://www.readbyqxmd.com/read/27935986/quantifying-adaptive-and-innate-immune-responses-in-hiv-infected-participants-using-a-novel-high-throughput-assay
#18
Michelle K Yong, Paul U Cameron, Tim Spelman, Julian H Elliott, Christopher K Fairley, Jeffrey Boyle, Misato Miyamasu, Sharon R Lewin
OBJECTIVES: HIV infection is characterised by persistent immune dysfunction of both the adaptive and innate immune responses. The aim of this study was to evaluate these responses using a novel high throughput assay in healthy controls and HIV-infected individuals prior to and following anti-retroviral treatment (ART). DESIGN: Cross-sectional study. METHODS: Whole blood was assessed using the QuantiFERON Monitor® (QFM) assay containing adaptive and innate immunostimulants...
2016: PloS One
https://www.readbyqxmd.com/read/27935985/mir-125b-enhances-il-8-production-in-early-onset-severe-preeclampsia-by-targeting-sphingosine-1-phosphate-lyase-1
#19
Weiwei Yang, Anning Wang, Chunling Zhao, Qinghua Li, Zhifang Pan, Xuefu Han, Cuijuan Zhang, Guohui Wang, Chao Ji, Guili Wang, Guangtao Jia, Jiyu Ju, Wei Gao, Wenjing Yu, Xiaoying Liu, Xi Chen, Weiguo Feng, Zhiqin Gao, Jie Li, Chune Ren
Preeclampsia (PE) is one of the leading causes of maternal and perinatal mortality and morbidity. One of the main hallmarks observed in PE is impaired inflammation state. In the current study, we found that miR-125b was deregulated in placental tissues and plasma derived from PE patients, which suggest a potential association between this miRNA and the pathogenesis of PE. Overexpression of miR-125b significantly reduced SGPL1 expression, and luciferase assays confirmed that SGPL1 is a direct target of miR-125b...
2016: PloS One
https://www.readbyqxmd.com/read/27935966/the-transcription-factor-nfatc2-regulates-%C3%AE-cell-proliferation-and-genes-associated-with-type-2-diabetes-in-mouse-and-human-islets
#20
Mark P Keller, Pradyut K Paul, Mary E Rabaglia, Donnie S Stapleton, Kathryn L Schueler, Aimee Teo Broman, Shuyun Isabella Ye, Ning Leng, Christopher J Brandon, Elias Chaibub Neto, Christopher L Plaisier, Shane P Simonett, Melkam A Kebede, Gloria M Sheynkman, Mark A Klein, Nitin S Baliga, Lloyd M Smith, Karl W Broman, Brian S Yandell, Christina Kendziorski, Alan D Attie
Human genome-wide association studies (GWAS) have shown that genetic variation at >130 gene loci is associated with type 2 diabetes (T2D). We asked if the expression of the candidate T2D-associated genes within these loci is regulated by a common locus in pancreatic islets. Using an obese F2 mouse intercross segregating for T2D, we show that the expression of ~40% of the T2D-associated genes is linked to a broad region on mouse chromosome (Chr) 2. As all but 9 of these genes are not physically located on Chr 2, linkage to Chr 2 suggests a genomic factor(s) located on Chr 2 regulates their expression in trans...
December 2016: PLoS Genetics
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