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Jaime prat

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https://www.readbyqxmd.com/read/29675313/high-sensitivity-and-label-free-oligonucleotides-detection-using-photonic-bandgap-sensing-structures-biofunctionalized-with-molecular-beacon-probes
#1
Ángela Ruiz-Tórtola, Francisco Prats-Quílez, Daniel González-Lucas, María-José Bañuls, Ángel Maquieira, Guy Wheeler, Tamas Dalmay, Amadeu Griol, Juan Hurtado, Jaime García-Rupérez
A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.
April 1, 2018: Biomedical Optics Express
https://www.readbyqxmd.com/read/29664230/experimental-study-of-the-evanescent-wave-photonic-sensors-response-in-presence-of-molecular-beacon-conformational-changes
#2
Ángela Ruiz-Tórtola, Francisco Prats-Quílez, Daniel Gónzalez-Lucas, María-José Bañuls, Ángel Maquieira, Guy Wheeler, Tamas Dalmay, Amadeu Griol, Juan Hurtado, Helge Bohlmann, Reiner Götzen, Jaime García-Rupérez
An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes -upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors- is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5' termination. Those MBs incorporate a biotin moiety close to their 3' termination in order to selectively bind a streptavidin molecule to them...
April 17, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29642479/real-time-seismic-data-from-the-bottom-sea
#3
Xavier Roset, Enric Trullols, Carola Artero-Delgado, Joana Prat, Joaquin Del Río, Immaculada Massana, Montserrat Carbonell, Jaime Barco de la Torre, Daniel Mihai Toma
An anchored marine seismometer, acquiring real-time seismic data, has been built and tested. The system consists of an underwater seismometer, a surface buoy, and a mooring line that connects them. Inductive communication through the mooring line provides an inexpensive, reliable, and flexible solution. Prior to the deployment the dynamics of the system have been simulated numerically in order to find optimal materials, cables, buoys, and connections under critical marine conditions. The seismometer used is a high sensitivity triaxial broadband geophone able to measure low vibrational signals produced by the underwater seismic events...
April 8, 2018: Sensors
https://www.readbyqxmd.com/read/29373718/the-aberrant-splicing-of-baf45d-links-splicing-regulation-and-transcription-in-glioblastoma
#4
Guillermo Aldave, Marisol Gonzalez-Huarriz, Angel Rubio, Juan Pablo Romero, Datta Ravi, Belén Miñana, Mar Cuadrado-Tejedor, Ana García-Osta, Roeland Verhaak, Enric Xipell, Naiara Martinez-Vélez, Arlet Acanda de la Rocha, Montserrat Puigdelloses, Marc García-Moure, Miguel Marigil, Jaime Gállego Pérez-Larraya, Oskar Marín-Bejar, Maite Huarte, Maria Stella Carro, Roberto Ferrarese, Cristobal Belda-Iniesta, Angel Ayuso, Ricardo Prat-Acín, Fernando Pastor, Ricardo Díez-Valle, Sonia Tejada, Marta M Alonso
Background: Glioblastoma, the most aggressive primary brain tumor, is a genetically heterogeneous tumor. Alternative spicing (AS) plays a key role in numerous pathologies, including cancer. The objective of our study was to determine whether aberrant AS could play a role in the malignant phenotype of glioma and to understand the mechanism underlying its aberrant regulation. Methods: We obtained surgical samples from patients with glioblastoma who underwent 5-aminolevulinic fluorescence-guided surgery...
January 24, 2018: Neuro-oncology
https://www.readbyqxmd.com/read/29245836/experimental-study-of-the-sensitivity-of-a-porous-silicon-ring-resonator-sensor-using-continuous-in-flow-measurements
#5
Raffaele Caroselli, Salvador Ponce-Alcántara, Francisco Prats Quilez, David Martín Sánchez, Luis Torrijos Morán, Amadeu Griol Barres, Laurent Bellieres, Hanna Bandarenka, Kseniya Girel, Vitaly Bondarenko, Jaime García-Rupérez
A highly sensitive photonic sensor based on a porous silicon ring resonator was developed and experimentally characterized. The photonic sensing structure was fabricated by exploiting a porous silicon double layer, where the top layer of a low porosity was used to form photonic elements by e-beam lithography and the bottom layer of a high porosity was used to confine light in the vertical direction. The sensing performance of the ring resonator sensor based on porous silicon was compared for the different resonances within the analyzed wavelength range both for transverse-electric and transverse-magnetic polarizations...
December 11, 2017: Optics Express
https://www.readbyqxmd.com/read/29206149/real-time-and-in-flow-sensing-using-a-high-sensitivity-porous-silicon-microcavity-based-sensor
#6
Raffaele Caroselli, David Martín Sánchez, Salvador Ponce Alcántara, Francisco Prats Quilez, Luis Torrijos Morán, Jaime García-Rupérez
Porous silicon seems to be an appropriate material platform for the development of high-sensitivity and low-cost optical sensors, as their porous nature increases the interaction with the target substances, and their fabrication process is very simple and inexpensive. In this paper, we present the experimental development of a porous silicon microcavity sensor and its use for real-time in-flow sensing application. A high-sensitivity configuration was designed and then fabricated, by electrochemically etching a silicon wafer...
December 5, 2017: Sensors
https://www.readbyqxmd.com/read/29164877/correction-to-the-discovery-of-novel-antimalarial-aminoxadiazoles-as-a-promising-nonendoperoxide-scaffold
#7
Elena Sandoval, María José Lafuente-Monasterio, María J Almela, Pablo Castañeda, María Belén Jiménez Díaz, María S Martínez-Martínez, Jaume Vidal, Íñigo Angulo-Barturen, Paul Bamborough, Jeremy Burrows, Nicholas Cammack, María J Chaparro, José M Coterón, Cristina de Cozar, Benigno Crespo, Beatriz Díaz, Gerard Drewes, Esther Fernández, Santiago Ferrer-Bazaga, María Teresa Fraile, Francisco J Gamo, Sonja Ghidelli-Disse, Rubén Gómez, John Haselden, Sophie Huss, María Luisa León, Jaime de Mercado, Simon J F Macdonald, José Ignacio Martín Hernando, Sara Prats, Margarita Puente, Anne Rodríguez, Juan C de la Rosa, Lourdes Rueda, Carolyn Selenski, Paul Willis, David M Wilson, Michael Witty, Félix Calderón
No abstract text is available yet for this article.
November 22, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29133142/dedifferentiated-endometrial-carcinomas-with-neuroendocrine-features-a-clinicopathologic-immunohistochemical-and-molecular-genetic-study
#8
Iñigo Espinosa, Antonio De Leo, Emanuela D'Angelo, Juan M Rosa-Rosa, Marina Corominas, Alan Gonzalez, José Palacios, Jaime Prat
Undifferentiated endometrial carcinoma is an aggressive type of uterine cancer which is occasionally associated with a low-grade endometrioid carcinoma component. This combination is referred to as "dedifferentiated endometrioid endometrial carcinoma." Neuroendocrine expression may occur in undifferentiated endometrial carcinoma but its significance in dedifferentiated endometrial carcinomas is unknown. To gain insight into the pathogenesis of these tumors we have analyzed the immunophenotype (ARID1A, MLH1, PMS2, MSH2, MSH6, p53, b-catenin, SMARCB1, synaptophysin, chromogranin A, and CD56) and mutational status (PTEN, KRAS, PIK3CA, TP53 and POLE) of 4 dedifferentiated endometrial carcinomas with strong and diffuse neuroendocrine expression...
November 10, 2017: Human Pathology
https://www.readbyqxmd.com/read/29123263/nuclear-igf-1r-predicts-chemotherapy-and-targeted-therapy-resistance-in-metastatic-colorectal-cancer
#9
Jordi Codony-Servat, Miriam Cuatrecasas, Elena Asensio, Carla Montironi, Anna Martínez-Cardús, Mercedes Marín-Aguilera, Carlos Horndler, Eva Martínez-Balibrea, Michele Rubini, Pedro Jares, Oscar Reig, Iván Victoria, Lydia Gaba, Marta Martín-Richard, Vicente Alonso, Pilar Escudero, Carlos Fernández-Martos, Jaime Feliu, Jose Carlos Méndez, Miguel Méndez, Javier Gallego, Antonieta Salud, Federico Rojo, Antoni Castells, Aleix Prat, Rafael Rosell, Xabier García-Albéniz, Jordi Camps, Joan Maurel
BACKGROUND: Although chemotherapy is the cornerstone treatment for patients with metastatic colorectal cancer (mCRC), acquired chemoresistance is common and constitutes the main reason for treatment failure. Monoclonal antibodies against insulin-like growth factor-1 receptor (IGF-1R) have been tested in pre-treated mCRC patients, but results have been largely deceiving. METHODS: We analysed time to progression, overall survival, and the mutational status of RAS, BRAF and nuclear p-IGF-1R expression by immunohistochemistry, in 470 metastatic CRC patients...
December 5, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29079180/mixed-endometrial-carcinomas-with-a-low-grade-serous-like-component-a-clinicopathologic-immunohistochemical-and-molecular-genetic-study
#10
Iñigo Espinosa, Emanuela D'Angelo, Marina Corominas, Alan Gonzalez, Jaime Prat
Recently, we reported 2 mixed endometrioid endometrial carcinomas with a "low-grade serous"-like component, which does not fit into any of the 4 molecular groups described by The Cancer Genome Atlas. To understand the nature of these tumors, we have done an immunohistochemical and molecular genetic study of these 2 cases and added a third case. Immunoreactivity for p53, ER, Ki67, WT1, MLH1, PMS2, MSH2, and MSH6 was assessed. Targeted next-generation sequencing for somatic mutations, including genes commonly implicated in carcinogenesis including TP53, KRAS, and PIK3CA, and Sanger sequencing for PTEN and POLE were also performed...
January 2018: Human Pathology
https://www.readbyqxmd.com/read/28806082/the-discovery-of-novel-antimalarial-aminoxadiazoles-as-a-promising-nonendoperoxide-scaffold
#11
Elena Sandoval, María José Lafuente-Monasterio, María J Almela, Pablo Castañeda, María Belén Jiménez Díaz, María S Martínez-Martínez, Jaume Vidal, Íñigo Angulo-Barturen, Paul Bamborough, Jeremy Burrows, Nicholas Cammack, María J Chaparro, José M Coterón, Cristina de Cozar, Benigno Crespo, Beatriz Díaz, Gerard Drewes, Esther Fernández, Santiago Ferrer-Bazaga, María Teresa Fraile, Francisco J Gamo, Sonja Ghidelli-Disse, Rubén Gómez, John Haselden, Sophie Huss, María Luisa León, Jaime de Mercado, Simon J F Macdonald, José Ignacio Martín Hernando, Sara Prats, Margarita Puente, Anne Rodríguez, Juan C de la Rosa, Lourdes Rueda, Carolyn Selenski, Paul Willis, David M Wilson, Michael Witty, Félix Calderón
Since the appearance of resistance to the current front-line antimalarial treatments, ACTs (artemisinin combination therapies), the discovery of novel chemical entities to treat the disease is recognized as a major global health priority. From the GSK antimalarial set, we identified an aminoxadiazole with an antiparasitic profile comparable with artemisinin (1), with no cross-resistance in a resistant strains panel and a potential new mode of action. A medicinal chemistry program allowed delivery of compounds such as 19 with high solubility in aqueous media, an acceptable toxicological profile, and oral efficacy...
August 24, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28601659/endometrioid-endometrial-carcinomas-with-microcystic-elongated-and-fragmented-melf-type-of-myoinvasion-role-of-immunohistochemistry-in-the-detection-of-occult-lymph-node-metastases-and-their-clinical-significance
#12
Iñigo Espinosa, Neus Serrat, Gian Franco Zannoni, Ramón Rovira, Emanuela D'Angelo, Jaime Prat
In endometrioid endometrial carcinomas (EECs), microcystic, elongated, and fragmented (MELF) myoinvasion is associated with easily overlooked lymph node metastases; however, the role of immunohistochemistry in their detection and their clinical significance have not been addressed. We identified MELF in 43 of 101 (43%) myoinvasive EECs. Nodes were removed in 49 (49%), 25 with MELF and 24 without MELF. Metastases were initially reported in 3 of the former (12%) and 2 of the latter (8%). All negative nodes were reviewed, and cytokeratin immunohistochemistry was performed...
December 2017: Human Pathology
https://www.readbyqxmd.com/read/28498284/undifferentiated-and-dedifferentiated-endometrial-carcinomas-with-pole-exonuclease-domain-mutations-have-a-favorable-prognosis
#13
Iñigo Espinosa, Cheng-Han Lee, Emanuela D'Angelo, José Palacios, Jaime Prat
POLE exonuclease domain mutations have recently been described in undifferentiated endometrial carcinoma but, because of the rarity of this aggressive type of endometrial cancer, their prognostic significance is unknown. We have analyzed the immunophenotype (ARID1A, MLH1, PMS2, MSH2, MSH6, p53, β-catenin, and SMARCB1) and mutational status (POLE, PIK3CA, and PTEN) of 21 undifferentiated carcinomas (8 undifferentiated and 13 dedifferentiated carcinomas). Loss of ARID1A expression was observed in 9 of 19 cases (47%), loss of expression of at least 1 DNA mismatch repair protein in 7 (7/21; 33%), and p53 immunoreaction was aberrant (mutated/inactivated) in 11 cases (11/21; 52%)...
August 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28277307/pathology-of-borderline-and-invasive-cancers
#14
REVIEW
Jaime Prat
Epithelial ovarian tumors are heterogeneous neoplasms primarily classified according to cell type. They are further subdivided into benign, borderline, and malignant (carcinomas), and this subdivision is very important as it correlates with behavior. Borderline ovarian tumors show epithelial proliferation higher than that seen in their benign counterparts and variable nuclear atypia; however, in contrast to carcinomas, there is no destructive stromal invasion, and their prognosis is much better. Ovarian carcinomas are the most common ovarian cancers and the most lethal gynecological malignancies...
May 2017: Best Practice & Research. Clinical Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/28078436/adhering-to-the-2014-who-terminology-on-borderline-ovarian-tumors
#15
COMMENT
Jaime Prat
No abstract text is available yet for this article.
February 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/27997699/chromatin-remodelling-and-dna-repair-genes-are-frequently-mutated-in-endometrioid-endometrial-carcinoma
#16
Pablo García-Sanz, Juan Carlos Triviño, Alba Mota, María Pérez López, Eva Colás, Alejandro Rojo-Sebastián, Ángel García, Sonia Gatius, María Ruiz, Jaime Prat, Rafael López-López, Miguel Abal, Antonio Gil-Moreno, Jaume Reventós, Xavier Matias-Guiu, Gema Moreno-Bueno
In developed countries, endometrial carcinoma is the most common cancer that affects the female genital tract. Endometrial carcinoma is divided into two main histological types, type I or endometrioid and type II or non-endometrioid, each of which have characteristic, although not exclusive, molecular alterations and mutational profiles. Nevertheless, information about the implication and relevance of some of these genes in this disease is lacking. We sought here to identify new recurrently mutated genes in endometrioid cancers that play a role in tumourigenesis and that influence the clinical outcome...
April 1, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/27895324/molecular-genetic-heterogeneity-in-undifferentiated-endometrial-carcinomas
#17
Juan M Rosa-Rosa, Susanna Leskelä, Eva Cristóbal-Lana, Almudena Santón, Ma Ángeles López-García, Gloria Muñoz, Belen Pérez-Mies, Michele Biscuola, Jaime Prat, Oliva E Esther, Robert A Soslow, Xavier Matias-Guiu, Jose Palacios
No abstract text is available yet for this article.
December 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/27491810/molecular-genetic-heterogeneity-in-undifferentiated-endometrial-carcinomas
#18
Juan M Rosa-Rosa, Susanna Leskelä, Eva Cristóbal-Lana, Almudena Santón, Ma Ángeles López-García, Gloria Muñoz, Belen Pérez-Mies, Michele Biscuola, Jaime Prat, Oliva E Esther, Robert A Soslow, Xavier Matias-Guiu, Jose Palacios
Undifferentiated and dedifferentiated endometrial carcinomas are rare and highly aggressive subtypes of uterine cancer, not well characterized at a molecular level. To investigate whether dedifferentiated carcinomas carry molecular genetic alterations similar to those of pure undifferentiated carcinomas, and to gain insight into the pathogenesis of these tumors, we selected a cohort of 18 undifferentiated endometrial carcinomas, 8 of them with a well-differentiated endometrioid carcinoma component (dedifferentiated endometrioid carcinomas), and studied them by immunohistochemistry and massive parallel and Sanger sequencing...
November 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/27346574/cd8-down-regulation-on-cytotoxic-t-lymphocytes-of-patients-with-endometrioid-endometrial-carcinomas
#19
Mónica Pascual-García, Cristina Bértolo, Juan C Nieto, Neus Serrat, Íñigo Espinosa, Emanuela D'Angelo, Raquel Muñoz, Ramón Rovira, Silvia Vidal, Jaime Prat
Carcinogenesis is a multistep process in which cancer cells and tumor stroma cells play important roles. T lymphocytes are immune constituents of tumor stroma and play a crucial function in anti-tumor response. By immunohistochemistry and flow cytometry, we studied T cytotoxic (CTLs) and T helper lymphocyte distribution and percentage in the tumor microenvironment and peripheral blood from 35 patients with endometrioid endometrial carcinomas (EEC). We also studied 23 healthy donors' blood samples as a control group...
October 2016: Human Pathology
https://www.readbyqxmd.com/read/26975040/mixed-and-ambiguous-endometrial-carcinomas-a-heterogenous-group-of-tumors-with-different-clinicopathologic-and-molecular-genetic-features
#20
Iñigo Espinosa, Emanuela D'Angelo, José Palacios, Jaime Prat
Besides endometrioid, serous, and clear cell carcinomas, there are endometrial carcinomas exhibiting mixed and ambiguous morphologic features. We have analyzed the immunophenotype (p53, p16, β-catenin, ER, HNF-1B, MLH1, and Ki-67) and mutational status (PTEN, KRAS, PIK3CA, and POLE) of 7 mixed carcinomas and 13 ambiguous carcinomas, all of them classified initially as mixed carcinomas. Only 2 of the 7 (28%) mixed carcinomas showed different immunophenotypes in different components. All but 2 tumors (5/7, 71%) overexpressed p53 and p16 and were negative for ER...
July 2016: American Journal of Surgical Pathology
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