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Seed Priming

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https://www.readbyqxmd.com/read/29774513/coordinated-effects-of-lead-toxicity-and-nutrient-deprivation-on-growth-oxidative-status-and-elemental-composition-of-primed-and-non-primed-rice-seedlings
#1
Fahad Khan, Saddam Hussain, Mohsin Tanveer, Sehrish Khan, Hafiz Athar Hussain, Biland Iqbal, Mingjian Geng
Rice crop is highly susceptible to the toxic levels of lead (Pb) during early growth stages. Moreover, a sufficient availability of mineral nutrients is critical for survival of plants particularly under stressful conditions. An experiment was carried out to unravel the coordinated effects of Pb stress (1-mM PbCl2 ) and different nutrient treatments (sufficient nutrient supply, nitrogen (N) deprivation, phosphorus (P) deprivation, and potassium (K) deprivation) on morphological growth, reactive oxygen species (ROS), antioxidants, and nutrient status in primed and non-primed rice seedlings...
May 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29763517/novel-engineered-tendon-fibrocartilage-bone-composite-with-cyclic-tension-for-rotator-cuff-repair
#2
Qian Liu, Taku Hatta, Jun Qi, Haoyu Liu, Andrew R Thoreson, Peter C Amadio, Steven L Moran, Scott P Steinmann, Anne Gingery, Chunfeng Zhao
Surgical repair of rotator cuff tears presents a significant clinical challenge with high failure rates and inferior functional outcomes. Graft augmentation improves repair outcomes, however currently available grafting materials have limitations. While cell-seeded decellularized tendon slices may facilitate cell infiltration, promote tendon incorporation and preserve original mechanical strength, the unique fibrocartilage zone is yet to be successfully reestablished. In this study, we investigated the biological and mechanical properties of an engineered tendon-fibrocartilage-bone composite (TFBC) with cyclic tension (3% strain, 0...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29679934/nanopriming-with-zero-valent-iron-nzvi-enhances-germination-and-growth-in-aromatic-rice-cultivar-oryza-sativa-cv-gobindabhog-l
#3
Titir Guha, K V G Ravikumar, Amitava Mukherjee, Anita Mukherjee, Rita Kundu
Engineered nanoparticles are utilized in agriculture for various purposes. They can be used as fertilizer, carrier for macro/micro nutrients or priming agents. Various nanoparticles are reported to have toxicity at very high doses, but at optimum concentration, they can be beneficial for plant growth and development. In the present study, low concentrations of nZVI nanoparticles were evaluated for their growth enhancement potential as seed priming agent in an aromatic rice cultivar, Oryza sativa cv. Gobindabhog...
April 12, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29652802/tunel-assay-and-dapi-staining-revealed-few-alterations-of-cellular-morphology-in-naturally-and-artificially-aged-seeds-of-cultivated-flax
#4
Yong-Bi Fu, Zaheer Ahmed, Hui Yang, Carolee Horbach
In a search for useful seed aging signals as biomarkers for seed viability prediction, we conducted an experiment using terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay and 4′,6-diamidino-2-phenylindole (DAPI) staining to analyze morphological and molecular changes in naturally aged (NA) and artificially aged (AA) flax ( Linum usitatissimum L.) seeds. A total of 2546 sections were performed from 112 seeds of 12 NA and AA seed samples with variable germination rates...
April 13, 2018: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29593496/q-rich-yeast-prion-psi-accelerates-aggregation-of-transthyretin-a-non-q-rich-human-protein
#5
Meenakshi Verma, Amandeep Girdhar, Basant Patel, Nirmal K Ganguly, Ritushree Kukreti, Vibha Taneja
Interactions amongst different amyloid proteins have been proposed as a probable mechanism of aggregation and thus an important risk factor for the onset as well as progression of various neurodegenerative disorders including Alzheimer's, Parkinson's, Huntington's, and Amyotrophic Lateral Sclerosis. Evidences suggest that transthyretin (TTR), a plasma protein associated with transthyretin amyloidosis or familial polyneuropathy (FAP) interacts with heterologous amyloid proteins including amyloid beta and islet amyloid polypeptide...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29587436/harnessing-the-power-of-t-cells-the-promising-hope-for-a-universal-influenza-vaccine
#6
REVIEW
E Bridie Clemens, Carolien van de Sandt, Sook San Wong, Linda M Wakim, Sophie A Valkenburg
Next-generation vaccines that utilize T cells could potentially overcome the limitations of current influenza vaccines that rely on antibodies to provide narrow subtype-specific protection and are prone to antigenic mismatch with circulating strains. Evidence from animal models shows that T cells can provide heterosubtypic protection and are crucial for immune control of influenza virus infections. This has provided hope for the design of a universal vaccine able to prime against diverse influenza virus strains and subtypes...
March 26, 2018: Vaccines
https://www.readbyqxmd.com/read/29577017/high-density-human-mesenchymal-stem-cell-rings-with-spatiotemporally-controlled-morphogen-presentation-as-building-blocks-for-engineering-bone-diaphyseal-tissue
#7
Samuel Herberg, Daniel Varghai, Yuxuan Cheng, Anna D Dikina, Phuong N Dang, Marsha W Rolle, Eben Alsberg
Emerging biomimetic tissue engineering strategies aim to partially recapitulate fundamental events that transpire during embryonic skeletal development; namely, cellular self-organization and targeted morphogenetic pathway activation. Here, we describe self-assembled, scaffold-free human mesenchymal stem cell (hMSC) rings featuring microparticle-mediated presentation of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2). We tested the hypothesis that spatiotemporally-controlled dual presentation of TGF-β1 and BMP-2 is superior in modulating in vitro endochondral ossification of high-density cellular constructs compared to single morphogen delivery...
2018: Nanotheranostics
https://www.readbyqxmd.com/read/29564630/proteomic-analysis-of-melatonin-mediated-osmotic-tolerance-by-improving-energy-metabolism-and-autophagy-in-wheat-triticum-aestivum-l
#8
Guibin Cui, Fengli Sun, Xinmei Gao, Kunliang Xie, Chao Zhang, Shudong Liu, Yajun Xi
Melatonin-mediated osmotic tolerance was attributed to increased antioxidant capacity, energy metabolism, osmoregulation and autophagy in wheat (Triticum aestivum L.). Melatonin is known to play multiple roles in plant abiotic stress tolerance. However, its role in wheat has been rarely investigated. In this study, 25% polyethylene glycol 6000 (PEG 6000) was used to simulate osmotic stress, and wheat seeds and seedlings were treated with different concentrations of melatonin under PEG stress. Isobaric tag for relative and absolute quantification (iTRAQ)-based proteomic techniques were used to identify the differentially accumulated proteins from melatonin-treated and non-treated seedlings...
March 21, 2018: Planta
https://www.readbyqxmd.com/read/29518353/nanoparticle-fusion-protein-complexes-protect-against-mycobacterium-tuberculosis-infection
#9
Peter Hart, Alastair Copland, Gil Reynolds Diogo, Shane Harris, Ralf Spallek, Wulf Oehlmann, Mahavir Singh, Juan Basile, Martin Rottenberg, Matthew John Paul, Rajko Reljic
Tuberculosis (TB) is the leading cause of death from infectious disease, and the current vaccine, Bacillus Calmette-Guerin (BCG), is inadequate. Nanoparticles (NPs) are an emerging vaccine technology, with recent successes in oncology and infectious diseases. NPs have been exploited as antigen delivery systems and also for their adjuvantic properties. However, the mechanisms underlying their immunological activity remain obscure. Here, we developed a novel mucosal TB vaccine (Nano-FP1) based upon yellow carnauba wax NPs (YC-NPs), coated with a fusion protein consisting of three Mycobacterium tuberculosis (Mtb) antigens: Acr, Ag85B, and HBHA...
March 7, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29515318/seed-priming-with-sorghum-extracts-and-benzyl-aminopurine-improves-the-tolerance-against-salt-stress-in-wheat-triticum-aestivum-l
#10
Ali Ahsan Bajwa, Muhammad Farooq, Ahmad Nawaz
Salt stress impedes the productivity of wheat ( Triticum aestivum L.) in many parts of the world. This study evaluated the potential role of benzyl aminopurine (BAP) and sorghum water extract (SWE) in improving the wheat performance under saline conditions. Seeds were primed with BAP (5 mg L-1 ), SWE (5% v/v), BAP + SWE, and distilled water (hydropriming). Soil filled pots maintained at the soil salinity levels of 4 and 10 dS m-1 were used for the sowing of primed and non-primed seeds. Salt stress suppressed the wheat growth; seed priming treatments significantly improved the wheat growth under optimal and suboptimal conditions...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29494674/post-mortem-findings-and-their-relation-to-aa-amyloidosis-in-free-ranging-herring-gulls-larus-argentatus
#11
Désirée S Jansson, Caroline Bröjer, Aleksija Neimanis, Torsten Mörner, Charles L Murphy, Faruk Otman, Per Westermark
Since the late 1990s, high mortality and declining populations have been reported among sea birds including Herring gulls (Larus argentatus) from the Baltic Sea area in Northern Europe. Repeated BoNT type C/D botulism outbreaks have occurred, but it remains unclear whether this is the sole and primary cause of mortality. Thiamine deficiency has also been suggested as a causal or contributing factor. With this study, we aimed to investigate gross and microscopic pathology in Herring gulls from affected breeding sites in Sweden in search of contributing diseases...
2018: PloS One
https://www.readbyqxmd.com/read/29487612/the-effect-of-storage-condition-and-duration-on-the-deterioration-of-primed-rice-seeds
#12
Weiqin Wang, Aibin He, Shaobing Peng, Jianliang Huang, Kehui Cui, Lixiao Nie
Seed priming is a successful practice to improve crop establishment under adverse environment. However, reduced longevity of primed rice ( Oryza sativa L.) seeds during storage limited the adoption of this technique. Present study investigated the effect of temperature, relative air humidity (RH) and oxygen on the longevity of primed rice seeds in a range of 60 days storage. In addition, the biochemical and morphological mechanisms associated with deterioration of primed seeds during storage were explored. Three types of priming treated rice seeds and one non-primed control were stored under (1) low temperature-vacuum (LT-V), (2) room temperature-vacuum (RT-V), (3) room temperature-aerobic-low RH (RT-A-LH) and (4) room temperature-aerobic- high RH (RT-A-HH) for 0, 15, 30, 45, and 60 days...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29453498/seed-priming-with-iron-and-zinc-in-bread-wheat-effects-in-germination-mitosis-and-grain-yield
#13
Sara Reis, Ivo Pavia, Ana Carvalho, José Moutinho-Pereira, Carlos Correia, José Lima-Brito
Currently, the biofortification of crops like wheat with micronutrients such as iron (Fe) and zinc (Zn) is extremely important due to the deficiencies of these micronutrients in the human diet and in soils. Agronomic biofortification with Fe and Zn can be done through different exogenous strategies such as soil application, foliar spraying, and seed priming. However, the excess of these micronutrients can be detrimental to the plants. Therefore, in the last decade, a high number of studies focused on the evaluation of their phytotoxic effects to define the best strategies for biofortification of bread wheat...
February 16, 2018: Protoplasma
https://www.readbyqxmd.com/read/29453255/endophyte-mediated-modulation-of-defense-related-genes-and-systemic-resistance-in-withania-somnifera-l-dunal-under-alternaria-alternata-stress
#14
Aradhana Mishra, Satyendra Pratap Singh, Sahil Mahfooz, Surendra Pratap Singh, Arpita Bhattacharya, Nishtha Mishra, C S Nautiyal
Endophytes have been explored and found to perform an important role in plant health. However, their effects on the host physiological function and disease management remain elusive. The present study aimed to assess the potential effects of endophytes, singly as well as in combination, in Withania somnifera (L.) Dunal, on various physiological parameters and systemic defense mechanisms against Alternaria alternata Seeds primed with the endophytic bacteria Bacillus amyloliquefaciens and Pseudomonas fluorescens individually and in combination demonstrated an enhanced vigor index and germination rate...
April 15, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29435762/establishment-of-a-myelinating-co-culture-system-with-a-motor-neuron-like-cell-line-nsc-34-and-an-adult-rat-schwann-cell-line-ifrs1
#15
Shizuka Takaku, Hideji Yako, Naoko Niimi, Tomoyo Akamine, Daiji Kawanami, Kazunori Utsunomiya, Kazunori Sango
Co-culture models of neurons and Schwann cells have been utilized for the study of myelination and demyelination in the peripheral nervous system; in most of the previous studies, however, these cells were obtained by primary culture with embryonic or neonatal animals. A spontaneously immortalized Schwann cell line IFRS1 from long-term cultures of adult Fischer rat peripheral nerves has been shown to retain fundamental ability to myelinate neurites in co-cultures with adult rat dorsal root ganglion neurons and nerve growth factor-primed PC12 cells...
February 12, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29416180/molecular-dynamics-in-germinating-endophyte-colonized-quinoa-seeds
#16
Andrea Pitzschke
Aims: The pseudo-cereal quinoa has an outstanding nutritional value. Seed germination is unusually fast, and plant tolerance to salt stress exceptionally high. Seemingly all seeds harbor bacterial endophytes. This work examines mitogen-activated protein kinase (MAPK) activities during early development. It evaluates possible contribution of endophytes to rapid germination and plant robustness. Methods: MAPK activities were monitored in water- and NaCl-imbibed seeds over a 4-h-period using an immunoblot-based approach...
January 2018: Plant and Soil
https://www.readbyqxmd.com/read/29407784/effect-of-selenium-induced-seed-priming-on-arsenic-accumulation-in-rice-plant-and-subsequent-transmission-in-human-food-chain
#17
Debojyoti Moulick, Subhas Chandra Santra, Dibakar Ghosh
The south-east Asian countries are facing a serious threat of arsenic (As) toxicity due to extensive use of As contaminated groundwater for rice cultivation. This experiment was configured to assess the consequences of rice seed priming with selenium (Se) and cultivation in As free and As contaminated soil. The experiment was arranged in a factorial complete randomized design having two factors viz. seed priming and soil As stress with total twenty-five treatment combinations replicated thrice. Seed priming with Se promotes growth, yield under both As free and As stressed conditions...
May 15, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29397260/vibration-induced-stress-priming-during-seed-culture-increases-microalgal-biomass-in-high-shear-field-cultivation
#18
Sang-Min Paik, EonSeon Jin, Sang Jun Sim, Noo Li Jeon
Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10-30 Hz and 0.15-0.45 G) was used to prime the cells for enhanced biomass. When exposed to 10 Hz at 0.15 G for 72 h and inoculated in baffled flasks of large shear fields (0...
April 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29331924/gene-expression-patterns-regulating-the-seed-metabolism-in-relation-to-deterioration-ageing-of-primed-mung-bean-vigna-radiata-l-seeds
#19
Satyendra Nath Sharma, Ankita Maheshwari, Chitra Sharma, Nidhi Shukla
We are proposing mechanisms to account for the loss of viability (seed deterioration/ageing) and enhancement in seed quality (post-storage priming treatment). In order to understand the regulatory mechanism of these traits, we conducted controlled deterioration (CD) test for up to 8 d using primed mung bean seeds and examined how CD effects the expression of many genes, regulating the seed metabolism in relation to CD and priming. Germination declined progressively with increased duration of CD, and the priming treatment completely/partially reversed the inhibition depending on the duration of CD...
March 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29310584/long-branch-chains-of-amylopectin-with-b-type-crystallinity-in-rice-seed-with-inhibition-of-starch-branching-enzyme-i-and-iib-resist-in-situ-degradation-and-inhibit-plant-growth-during-seedling-development-degradation-of-rice-starch-with-inhibition-of-sbei
#20
Ting Pan, Lingshang Lin, Juan Wang, Qiaoquan Liu, Cunxu Wei
BACKGROUND: Endosperm starch provides prime energy for cereal seedling growth. Cereal endosperm with repression of starch branching enzyme (SBE) has been widely studied for its high resistant starch content and health benefit. However, in barley and maize, the repression of SBE changes starch component and amylopectin structure which affects grain germination and seedling establishment. A high resistant starch rice line (TRS) has been developed through inhibiting SBEI/IIb, and its starch has very high resistance to in vitro hydrolysis and digestion...
January 8, 2018: BMC Plant Biology
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