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Seed Priming

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https://www.readbyqxmd.com/read/28196091/pre-breeding-of-lentil-lens-culinaris-medik-for-herbicide-resistance-through-seed-mutagenesis
#1
Muhammad Rizwan, Muhammad Aslam, Muhammad Jawad Asghar, Ghulam Abbas, Tariq Mahmud Shah, Hussein Shimelis
Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28...
2017: PloS One
https://www.readbyqxmd.com/read/28157493/soil-primes-the-seed-epigenetic-landscape-drives-tumor-behavior
#2
Ramon J Whitson, Anthony E Oro
Whether invasive tumor phenotypes like EMT arise from oncogenic drivers or from priming of the pre-tumor cell of origin remains unknown. In this issue of Cell Stem Cell, Latil et al. (2017) show that the pre-tumor niche establishes a chromatin state predisposing squamous cell carcinomas to undergo EMT and metastasis, suggesting that the pre-tumor epigenome has prognostic value.
February 2, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28128224/pyrintegrin-induces-soft-tissue-formation-by-transplanted-or-endogenous-cells
#3
Bhranti S Shah, Mo Chen, Takahiro Suzuki, Mildred Embree, Kimi Kong, Chang H Lee, Ling He, Lusai Xiang, Jeffrey A Ahn, Sheng Ding, Jeremy J Mao
Focal adipose deficiency, such as lipoatrophy, lumpectomy or facial trauma, is a formidable challenge in reconstructive medicine, and yet scarcely investigated in experimental studies. Here, we report that Pyrintegrin (Ptn), a 2,4-disubstituted pyrimidine known to promote embryonic stem cells survival, is robustly adipogenic and induces postnatal adipose tissue formation in vivo of transplanted adipose stem/progenitor cells (ASCs) and recruited endogenous cells. In vitro, Ptn stimulated human adipose tissue derived ASCs to differentiate into lipid-laden adipocytes by upregulating peroxisome proliferator-activated receptor (PPARγ) and CCAAT/enhancer-binding protein-α (C/EBPα), with differentiated cells increasingly secreting adiponectin, leptin, glycerol and total triglycerides...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28114972/optimizing-mesoderm-progenitor-selection-and-three-dimensional-microniche-culture-allows-highly-efficient-endothelial-differentiation-and-ischemic-tissue-repair-from-human-pluripotent-stem-cells
#4
Fengzhi Zhang, Lin Wang, Yaqian Li, Wei Liu, Fuyu Duan, Rujin Huang, Xi Chen, Sophia Chia-Ning Chang, Yanan Du, Jie Na
BACKGROUND: Generation of large quantities of endothelial cells is highly desirable for vascular research, for the treatment of ischemia diseases, and for tissue regeneration. To achieve this goal, we developed a simple, chemically defined culture system to efficiently and rapidly differentiate endothelial cells from human pluripotent stem cells by going through an MESP1 mesoderm progenitor stage. METHODS: Mesp1 is a key transcription factor that regulates the development of early cardiovascular tissue...
January 23, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28093607/osmopriming-induced-salt-tolerance-during-seed-germination-of-alfalfa-most-likely-mediates-through-h2o2-signaling-and-upregulation-of-heme-oxygenase
#5
Rayhaneh Amooaghaie, Fatemeh Tabatabaie
The present study showed that osmopriming or pretreatment with low H2O2 doses (2 mM) for 6 h alleviated salt-reduced seed germination. The NADPH oxidase activity was the main source, and superoxide dismutase (SOD) activity might be a secondary source of H2O2 generation during osmopriming or H2O2 pretreatment. Hematin pretreatment similar to osmopriming improved salt-reduced seed germination that was coincident with the enhancement of heme oxygenase (HO) activity. The semi-quantitative RT-PCR confirmed that osmopriming or H2O2 pretreatment was able to upregulate heme oxygenase HO-1 transcription, while the application of N,N-dimethyl thiourea (DMTU as trap of endogenous H2O2) and diphenyleneiodonium (DPI as inhibitor of NADPHox) not only blocked the upregulation of HO but also reversed the osmopriming-induced salt attenuation...
January 16, 2017: Protoplasma
https://www.readbyqxmd.com/read/28090647/the-multifaceted-role-of-extracellular-vesicles-in-metastasis-priming-the-soil-for-seeding
#6
REVIEW
Brunna Dos Anjos Pultz, Felipe Andrés Cordero da Luz, Sara Socorro Faria, Leandro Peixoto Ferreira de Souza, Paula Cristina Brígido Tavares, Vivian Alonso Goulart, Wagner Fontes, Luiz Ricardo Goulart, Marcelo José Barbosa Silva
Extracellular vesicles (EVs), including exosomes, play a key role in inter and intracellular communication, promoting the proliferation and invasion of recipient cells to support tumor growth and metastasis. Metastasis comprises multiple steps that first include the detachment of tumor cells through epithelial to mesenchymal transition (EMT), allowing the physical dissemination to distant organs. Thereafter, cancer-derived exosomes are still critical components for preparing the tumor microenvironment by (i) enabling tumor cells to escape from the immunological surveillance and (ii) arranging the pre-metastatic site for the engraftment of detached cancer cells...
January 16, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28061743/shotgun-proteomics-of-the-barley-seed-proteome
#7
Ramamurthy Mahalingam
BACKGROUND: Barley seed proteins are of prime importance to the brewing industry, human and animal nutrition and in plant breeding for cultivar identification. To obtain comprehensive proteomic data from seeds, total protein from a two-rowed (Conrad) and a six-rowed (Lacey) barley cultivar were precipitated in acetone, digested in-solution, and the resulting peptides were analyzed by nano-liquid chromatography coupled with tandem mass spectrometry. RESULTS: The raw mass spectra data searched against Uniprot's Barley database using in-house Mascot search engine identified 1168 unique proteins...
January 6, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28056386/h2o2-seed-priming-improves-tolerance-to-salinity-drought-and-their-combined-effect-more-than-mannitol-in-cakile-maritima-when-compared-to-eutrema-salsugineum
#8
Hasna Ellouzi, Souhir Sghayar, Chedly Abdelly
The effect of H2O2 and mannitol seed priming was investigated on plant growth, oxidative stress biomarkers and activities of antioxidant enzymes in leaves of Cakile maritima and Eutrema salsugineum, when exposed to drought and salt stress, either separately applied or combined. Under unprimed conditions, drought severely restricted growth (40% as compared to the control) and redox balance of C. maritima seedlings, whereas E. salsugineum showed these drastic effects under individual salinity (33% as compared to the control)...
December 15, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28054268/optimizing-the-phosphorus-use-in-cotton-by-using-csm-cropgro-cotton-model-for-semi-arid-climate-of-vehari-punjab-pakistan
#9
Asad Amin, Wajid Nasim, Muhammad Mubeen, Muhammad Nadeem, Liaqat Ali, Hafiz Mohkum Hammad, Syeda Refat Sultana, Khawar Jabran, M Habib Ur Rehman, Shakeel Ahmad, Muhammad Awais, Atta Rasool, Shah Fahad, Shah Saud, Adnan Noor Shah, Zahid Ihsan, Shahzad Ali, Ali Ahsan Bajwa, Khalid Rehman Hakeem, Asif Ameen, Amanullah, Hafeez Ur Rehman, Fahad Alghabar, Ghulam Hussain Jatoi, Muhammad Akram, Aziz Khan, Faisal Islam, Syed Tahir Ata-Ul-Karim, Muhammad Ishaq Asif Rehmani, Sajid Hussain, Muhammad Razaq, Amin Fathi
Crop nutrient management is an essential component of any cropping system. With increasing concerns over environmental protection, improvement in fertilizer use efficiencies has become a prime goal in global agriculture system. Phosphorus (P) is one of the most important nutrients, and strategies are required to optimize its use in important arable crops like cotton (Gossypium hirsutum L.) that has great significance. Sustainable P use in crop production could significantly avoid environmental hazards resulting from over-P fertilization...
January 4, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28049533/inflammatory-pre-conditioning-restricts-the-seeded-induction-of-%C3%AE-synuclein-pathology-in-wild-type-mice
#10
Emily J Koller, Mieu M T Brooks, Todd E Golde, Benoit I Giasson, Paramita Chakrabarty
BACKGROUND: Cell-to-cell transmission of α-synuclein (αSyn) is hypothesized to play an important role in disease progression in synucleinopathies. This process involves cellular uptake of extracellular amyloidogenic αSyn seeds followed by seeding of endogenous αSyn. Though it is well known that αSyn is an immunogenic protein that can interact with immune receptors, the role of innate immunity in regulating induction of αSyn pathology in vivo is unknown. Herein, we explored whether altering innate immune activation affects induction of αSyn pathology in wild type mice...
January 3, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28035152/pulsed-magnetic-field-improves-seed-quality-of-aged-green-pea-seeds-by-homeostasis-of-free-radical-content
#11
Jyotsna Bhardwaj, Anjali Anand, V K Pandita, Shantha Nagarajan
To elucidate the mechanism responsible for magnetic field induced seed invigoration in aged seeds an experiment was conducted on six year old garden pea seeds stored under controlled (20 °C and 40% RH) condition. Aged seeds were magnetoprimed by exposing to pulsed magnetic field (PMF) of 100 mT for 1 h in three pulsed modes. The 6 min on and off PMF showed significant improvement in germination (7.6%) and vigor (84.8%) over aged seeds. Superoxide and hydrogen peroxide production increased in germinating primed seeds by 27 and 52%, respectively, over aged seeds...
November 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28009562/synchrotron-based-phase-sensitive-imaging-of%C3%A2-leaves-grown-from-magneto-primed-seeds-of-soybean
#12
A Fatima, S Kataria, L Baghel, K N Guruprasad, A K Agrawal, B Singh, P S Sarkar, T Shripathi, Y Kashyap
Experiments were conducted to study the effects of static magnetic fields (SMFs) on the venation network of soybean leaves using the synchrotron-based X-ray micro-imaging technique. The seeds of soybean (Glycine max, variety JS-335) were pretreated with different SMFs from 50 to 300 mT in steps of 50 mT for 1 h. The phase-contrast images obtained showed that, as the strength of the SMF increased, the area, width of the midrib, area of the midrib and minor vein of the middle leaflets of third trifoliate leaves also increased up to the SMF strength of 200 mT (1 h) and decreased thereafter...
January 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/28004101/salicylic-acid-and-calcium-treatments-improves-wheat-vigor-lipids-and-phenolics-under-high-salinity
#13
Nilgün Yücel Candan, Haklı Heybet Elif
Seed vigor is a complex physiological trait required to ensure the rapid and uniform emergence of plants in the field under different environmental conditions. Therefore, salicylic acid (SA, 0.5 mM) and calcium (Ca2+, 50 mM) priming were used as exogenous growth enhancers to stimulate wheat (Triticum durum Desf. cv. Yelken) seed vigor under high salinity. The main aim was to address whether priming of wheat with SA, Ca2+ and SA+Ca (SA, 0.5 mM + Ca2+, 50 mM; their combination) could bring about supplementary agronomic benefits particularly under stressful environments such as salinity...
December 2016: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/27997597/immunogenicity-and-protective-efficacy-of-the-dar-901-booster-vaccine-in-a-murine-model-of-tuberculosis
#14
Timothy Lahey, Dominick Laddy, Krystal Hill, Jacqueline Schaeffer, Alison Hogg, James Keeble, Belinda Dagg, Mei Mei Ho, Robert D Arbeit, C Fordham von Reyn
BACKGROUND: The development of a novel tuberculosis vaccine is a leading global health priority. SRL172, an inactivated, whole-cell mycobacterial vaccine, was safe, immunogenic and reduced the incidence of culture-confirmed tuberculosis in a phase III trial in HIV-infected and BCG immunized adults in Tanzania. Here we describe the immunogenicity and protective efficacy of DAR-901, a booster vaccine against tuberculosis manufactured from the same seed strain using a new scalable method...
2016: PloS One
https://www.readbyqxmd.com/read/27987472/seed-priming-improves-chilling-tolerance-in-chickpea-by-modulating-germination-metabolism-trehalose-accumulation-and-carbon-assimilation
#15
Muhammad Farooq, Mubshar Hussain, Ahmad Nawaz, Dong-Jin Lee, Salem S Alghamdi, Kadambot H M Siddique
Chilling stress is one of the major abiotic stresses affecting chickpea productivity worldwide. This study evaluated the potential role of seed priming in improving resistance to chilling stress in chickpea (cv. Punjab, 2008). The priming treatments involved soaking seeds of chickpea cultivar Punjab 2008 in either water for 8 h (on-farm priming), aerated water (hydropriming) for 18 h, or CaCl2 solution (ψs -1.25 MPa; osmopriming) for 18 h. Primed and untreated seeds were grown either at 18/15 °C (control) or 13/10 °C (chilling stress)...
December 9, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27927886/biostability-of-the-proanthocyanidins-dentin-complex-and-adhesion-studies
#16
A A Leme-Kraus, B Aydin, C M P Vidal, R M Phansalkar, J W Nam, J McAlpine, G F Pauli, S Chen, A K Bedran-Russo
Oligomeric proanthocyanidins (OPACs) are potent and renewable natural bioactives possible to be refined into chemically standardized mixtures for biological applications. Herein, we found that multiscale interactions of OPACs with the dentin matrix create tight biointerfaces with hydrophobic methacrylate adhesives on wet surfaces. An enriched mixture of OPACs, with a known phytochemical profile, was produced from grape seed crude extract (Vitis vinifera; enriched grape seed extract [e-GSE]) and applied to dentin matrices to determine changes to the mechanical properties and biodegradability of the dentin matrix and favorable resin adhesion mechanisms...
December 7, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27922667/pituitary-sex-hormones-enhance-the-pro%C3%A2-metastatic-potential-of-human-lung-cancer-cells-by-downregulating-the-intracellular-expression-of-heme-oxygenase%C3%A2-1
#17
Ahmed Abdelbaset-Ismail, Daniel Pedziwiatr, Gabriela Schneider, Jacek Niklinski, Radoslaw Charkiewicz, Marcin Moniuszko, Magda Kucia, Mariusz Z Ratajczak
We report that human lung cancer cell lines express functional receptors for pituitary sex hormones (SexHs) and respond to stimulation by follicle‑stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL). Expression of these receptors has also been confirmed in patient lung cancer samples at the mRNA level. Stimulation of human lung cancer cell lines with FSH, LH, or PRL stimulated migration and chemotaxis, and some cell lines responded by enhanced proliferation. Moreover, priming of human lung cancer cells by exposing them to pituitary SexHs resulted in enhanced seeding efficiency of injected human lung cancer cells into bone marrow, liver, and lungs in an immunodeficient mouse model...
January 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/27867393/melatonin-application-to-pisum-sativum-l-seeds-positively-influences-the-function-of-the-photosynthetic-apparatus-in-growing-seedlings-during-paraquat-induced-oxidative-stress
#18
Katarzyna Szafrańska, Russel J Reiter, Małgorzata M Posmyk
Melatonin, due to its pleiotropic effects plays an important role improving tolerance to stresses. Plants increase endogenous melatonin synthesis when faced with harsh environments as well as exogenously applied melatonin limits stress injuries. Presented work demonstrated that single melatonin application into the seeds during pre-sowing priming improved oxidative stress tolerance of growing seedlings exposed to paraquat (PQ). PQ is a powerful herbicide which blocks the process of photosynthesis under light conditions due to free radicals excess production, when O2 is rapidly converted to [Formula: see text] and subsequently to other reactive oxygen species...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27865799/oleic-acid-induced-angptl4-enhances-head-and-neck-squamous-cell-carcinoma-anoikis-resistance-and-metastasis-via-up-regulation-of-fibronectin
#19
Chih-Jie Shen, Shih-Hung Chan, Chung-Ta Lee, Wan-Chen Huang, Jhih-Peng Tsai, Ben-Kuen Chen
Obese patients have higher levels of free fatty acids (FFAs) in their plasma and a higher risk of cancer than their non-obese counterparts. However, the mechanisms involved in the regulation of cancer metastasis by FFAs remain unclear. In this study, we found that oleic acid (OA) induced angiopoietin-like 4 (ANGPTL4) protein expression and secretion and conferred anoikis resistance to head and neck squamous cell carcinomas (HNSCCs). The autocrine production of OA-induced ANGPTL4 further promoted HNSCC migration and invasion...
February 1, 2017: Cancer Letters
https://www.readbyqxmd.com/read/27833081/laricitrin-ameliorates-lung-cancer-mediated-dendritic-cell-suppression-by-inhibiting-signal-transducer-and-activator-of-transcription-3
#20
Wei-An Chang, Jen-Yu Hung, Shu-Fang Jian, Yi-Shiuan Lin, Cheng-Ying Wu, Ya-Ling Hsu, Po-Lin Kuo
Natural polyphenolic compounds of grapes and their seeds are thought to be therapeutic adjuvants in a variety of diseases, including cancer prevention. This study was carried out to investigate the effect of grape phenolic compounds on the regulation of cancer-mediated immune suppression. Laricitrin exhibits the greatest potential to ameliorate the suppressive effects of lung cancer on dendritic cells' (DCs') differentiation, maturation and function. Human lung cancer A549 and CL1-5 cells change the phenotype of DCs that express to high levels of IL-10 and prime T cells towards an immune suppression type-2 response (Th2)...
November 9, 2016: Oncotarget
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