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https://www.readbyqxmd.com/read/28550091/transgenic-expression-of-il15-improves-antiglioma-activity-of-il13ralpha2-car-t-cells-but-results-in-antigen-loss-variants
#1
Giedre Krenciute, Brooke L Prinzing, Zhongzhen Yi, Meng-Fen Wu, Hao Liu, Gianpietro Dotti, Irina V Balyasnikova, Stephen Gottschalk
Glioblastoma (GBM) is the most aggressive primary brain tumor in adults and is virtually incurable with conventional therapies. Immunotherapy with T cells expressing GBM-specific chimeric antigen receptors (CARs) is an attractive approach to improve outcomes. Although CAR T cells targeting GBM antigens such as IL13Ralpha2 (interleukin 13 Receptor Subunit Alpha 2), HER2 (human epidermal growth factor receptor 2), and EGFRvIII (epidermal growth factor receptor variant III) have had antitumor activity in preclinical models, early phase clinical testing has demonstrated limited antiglioma activity...
May 26, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/28549559/the-novel-indole-3-formaldehyde-2-aitfei-3-f-is-involved-in-processes-of-apoptosis-induction
#2
Mojgan Noroozi Karimabad, Mehdi Mahmoodi, Abdollah Jafarzadeh, Ali Darehkordi, Mohammad Reza Hajizadeh, Hossein Khorramdelazad, Soudeh Khanamani Falahati-Pour, Gholamhossein Hassanshahi
AIM AND OBJECTIVES: Balancing between Bax and Bcl-2 plays critical roles in both proliferation and self-renewal activation of cancer cells. Indole-3-formaldehyde derivatives limit the growth and facilitate cell death in different cell systems. In this study, we introduced a novel indole derivative (2-AITFEI-3-F) with tendency to facilitate apoptosis in NB4 line in comparison to basal Indole-3-formaldehyde (I3F). METHODS: The NB4 cells were cultured in RPMI1640 medium contained 2-AITFEI-3-F and I3F (15...
May 23, 2017: Life Sciences
https://www.readbyqxmd.com/read/28549252/alkaline-and-oxidative-pretreatments-for-the-anaerobic-digestion-of-cow-manure-and-maize-straw-factors-influencing-the-process-and-preliminary-economic-viability-of-an-industrial-application
#3
Juan Luis Ramos-Suárez, Daniel Gómez, Leticia Regueiro, Andrea Baeza, Felipe Hansen
This paper studies the application of calcium oxide (CaO), peracetic acid (PAA) and a combination of both in order to reduce lignin content and increase biogas potential of cow manure and maize straw. Changes in organic matter were mainly affected by the type of reagent use and the dosage, with minimum influence of exposure time and dilution. Changes in pH may limit the application of chemicals. Increase in biogas production with a combination of CaO and PAA, and separate application of PAA and CaO was 156...
May 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28548167/light-induced-antibiotic-release-from-a-coumarin-caged-compound-on-the-ultrafast-timescale
#4
L-M Herzig, I Elamri, H Schwalbe, J Wachtveitl
A synthesis route for puromycin caged with the photo-responsive 7-diethylaminocoumarinyl protecting group carbamate was developed. The inactivation and recovery of the cytotoxic effect of puromycin was tested with a XTT cell viability assay. The uncaging mechanism was studied by ultrafast transient absorption spectroscopy and by time-correlated single photon counting. The combination of these results with quantum-chemical calculations provided detailed insights in dynamics upon excitation. Interestingly, a change of the dipole moment due to structural rearrangements of the amino moiety led to an intermolecular charge transfer on the picosecond time-scale...
May 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28546541/protective-effect-of-low-dose-intra-articular-cadmium-on-inflammation-and-joint-destruction-in-arthritis
#5
Paola Bonaventura, Guillaume Courbon, Aline Lamboux, Fabien Lavocat, Hubert Marotte, Francis Albarède, Pierre Miossec
Synovium hyperplasia characterizes joint diseases, such as rheumatoid arthritis (RA). The cytotoxic effect of low-dose Cadmium (Cd) was tested in vitro and ex vivo on synoviocytes, the mesenchymal key effector cells of inflammation and proliferation in arthritis. The anti-inflammatory and anti-proliferative effects of Cd were tested in vivo by intra-articular injection in the adjuvant induced arthritis rat joints, where the clinical scores and the consequences of arthritis were evaluated. Cell death through apoptosis was highly induced by Cd in inflammatory synoviocytes (80% reduction of cell viability, p < 0...
May 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28545072/effect-of-vitamin-d-on-endothelial-progenitor-cells-function
#6
Yoav Hammer, Alissa Soudry, Amos Levi, Yeela Talmor-Barkan, Dorit Leshem-Lev, Joel Singer, Ran Kornowski, Eli I Lev
BACKGROUND: Endothelial progenitor cells (EPCs) are a population of bone marrow-derived cells, which have an important role in the process of endothelialization and vascular repair following injury. Impairment of EPCs, which occurs in patients with diabetes, was shown to be related to endothelial dysfunction, coronary artery disease (CAD) and adverse clinical outcomes. Recent evidence has shown that calcitriol, the active hormone of vitamin D, has a favorable impact on the endothelium and cardiovascular system...
2017: PloS One
https://www.readbyqxmd.com/read/28544579/frizzled7-antibody-functionalized-nanoshells-enable-multivalent-binding-for-wnt-signaling-inhibition-in-triple-negative-breast-cancer-cells
#7
Rachel S Riley, Emily S Day
Antibodies that antagonize cell signaling pathways specific to their targeted receptor are invaluable tools to study and treat malignancies, but their utility is limited by high production costs and treatment dosages. Researchers have shown that antibodies conjugated to nanoparticles display increased affinity for their target relative to freely delivered antibodies due to multivalency, and this study investigates how this multivalency can enable antibody-nanoparticle conjugates to inhibit oncogenic cell signaling more effectively than freely delivered antibodies...
May 22, 2017: Small
https://www.readbyqxmd.com/read/28542705/a-pump-free-microfluidic-3d-perfusion-platform-for-the-efficient-differentiation-of-human-hepatocyte-like-cells
#8
Louis Jun Ye Ong, Lor Huai Chong, Lin Jin, Pawan Kumar Singh, Poh Seng Lee, Hanry Yu, Abhishek Ananthanarayanan, Hwa Liang Leo, Yi-Chin Toh
The practical application of microfluidic liver models for in vitro drug testing is partly hampered by their reliance on human primary hepatocytes, which are limited in number and have batch-to-batch variation. Human stem cell-derived hepatocytes offer an attractive alternative cell source, although their 3D differentiation and maturation in a microfluidic platform have not yet been demonstrated. We develop a pump-free microfluidic 3D perfusion platform to achieve long-term and efficient differentiation of human liver progenitor cells into hepatocyte-like cells (HLCs)...
May 20, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28540295/relaxin-attenuates-contrast-induced-human-proximal-tubular-epithelial-cell-apoptosis-by-activation-of-the-pi3k-akt-signaling-pathway-in-vitro
#9
Xiang-Cheng Xie, Yizhi Cao, Xiu Yang, Qun-Hong Xu, Wei Wei, Ming Wang
Background. Contrast-induced acute kidney injury (CI-AKI) is one of the main causes of iatrogenic acute kidney injury (AKI); however, therapeutic strategies for AKI remain limited. This study aims to explore the effect of relaxin (RLX) on contrast-induced HK-2 apoptosis and its underlying mechanisms. Methods. Renal tubular epithelial cells (HK-2) were incubated either with or without ioversol, human H2 relaxin, and LY294002 (the inhibitor of the PI3K/Akt signal pathway). Cell viability was evaluated with a CCK-8 assay...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28537595/engineering-live-cell-surfaces-with-functional-polymers-via-cytocompatible-controlled-radical-polymerization
#10
Jia Niu, David J Lunn, Anusha Pusuluri, Justin I Yoo, Michelle A O'Malley, Samir Mitragotri, H Tom Soh, Craig J Hawker
The capability to graft synthetic polymers onto the surfaces of live cells offers the potential to manipulate and control their phenotype and underlying cellular processes. Conventional grafting-to strategies for conjugating preformed polymers to cell surfaces are limited by low polymer grafting efficiency. Here we report an alternative grafting-from strategy for directly engineering the surfaces of live yeast and mammalian cells through cell surface-initiated controlled radical polymerization. By developing cytocompatible PET-RAFT (photoinduced electron transfer-reversible addition-fragmentation chain-transfer polymerization), synthetic polymers with narrow polydispersity (Mw/Mn < 1...
June 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28536700/fabrication-and-evaluation-of-electrospun-3d-bioplotted-and-combination-of-electrospun-3d-bioplotted-scaffolds-for-tissue-engineering-applications
#11
Liliana F Mellor, Pedro Huebner, Shaobo Cai, Mahsa Mohiti-Asli, Michael A Taylor, Jeffrey Spang, Rohan A Shirwaiker, Elizabeth G Loboa
Electrospun scaffolds provide a dense framework of nanofibers with pore sizes and fiber diameters that closely resemble the architecture of native extracellular matrix. However, it generates limited three-dimensional structures of relevant physiological thicknesses. 3D printing allows digitally controlled fabrication of three-dimensional single/multimaterial constructs with precisely ordered fiber and pore architecture in a single build. However, this approach generally lacks the ability to achieve submicron resolution features to mimic native tissue...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28534597/metabolomics-for-improving-pregnancy-outcomes-in-women-undergoing-assisted-reproductive-technologies
#12
REVIEW
Charalampos S Siristatidis, Eleni Sertedaki, Dennis Vaidakis
BACKGROUND: In order to overcome the low effectiveness of assisted reproductive technologies (ART) and the high incidence of multiple births, metabolomics is proposed as a non-invasive method to assess oocyte quality, embryo viability, and endometrial receptivity, and facilitate a targeted subfertility treatment. OBJECTIVES: To evaluate the effectiveness and safety of metabolomic assessment of oocyte quality, embryo viability, and endometrial receptivity for improving live birth or ongoing pregnancy rates in women undergoing ART, compared to conventional methods of assessment...
May 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28534592/thermo-electrochemical-cells-for-waste-heat-harvesting-progress-and-perspectives
#13
M F Dupont, D R MacFarlane, J M Pringle
Thermo-electrochemical cells (also called thermocells) are promising devices for harvesting waste heat for the sustainable production of energy. Research into thermocells has increased significantly in recent years, driven by advantages such as their ability to continuously convert heat into electrical energy without producing emissions or consuming materials. Until relatively recently, the commercial viability of thermocells was limited by their low power output and conversion efficiency. However, there have lately been significant advances in thermocell performance as a result of improvements to the electrode materials, electrolyte and redox chemistry and various features of the cell design...
May 23, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28534057/the-effect-of-thawing-protocols-on-follicle-conservation-in-human-ovarian-tissue-cryopreservation
#14
T Morewood, N Getreu, B Fuller, J Morris, P Hardiman
BACKGROUND: Ovarian tissue cryopreservation has the potential to improve fertility preservation for a growing number of patients undergoing sterilising therapy, particularly where oocyte or embryo cryopreservation is not suitable. However, its success is limited by significant follicular apoptosis upon thawing, and there is wide variation in thawing protocols used with little evidence of efficacy. OBJECTIVE: To determine the best warming rates to maintain tissue viability...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28533959/preservation-media-durations-and-cell-concentrations-of-short-term-storage-affect-key-features-of-human-adipose-derived-mesenchymal-stem-cells-for-therapeutic-application
#15
Fengli Zhang, Huaijuan Ren, Xiaohu Shao, Chao Zhuang, Yantian Chen, Nianmin Qi
BACKGROUND: Adipose-derived mesenchymal stem cells (ADSCs) have shown great potential in the treatment of various diseases. However, the optimum short-term storage condition of ADSCs in 2∼8 °C is rarely reported. This study aimed at optimizing a short-term storage condition to ensure the viability and function of ADSCs before transplantation. METHODS: Preservation media and durations of storage were evaluated by cell viability, apoptosis, adhesion ability and colony-forming unit (CFU) capacity of ADSCs...
2017: PeerJ
https://www.readbyqxmd.com/read/28532179/capturing-context-related-change-in-emotional-dynamics-via-fixed-moderated-time-series-analysis
#16
Janne K Adolf, Manuel C Voelkle, Annette Brose, Florian Schmiedek
Much of recent affect research relies on intensive longitudinal studies to assess daily emotional experiences. The resulting data are analyzed with dynamic models to capture regulatory processes involved in emotional functioning. Daily contexts, however, are commonly ignored. This may not only result in biased parameter estimates and wrong conclusions, but also ignores the opportunity to investigate contextual effects on emotional dynamics. With fixed moderated time series analysis, we present an approach that resolves this problem by estimating context-dependent change in dynamic parameters in single-subject time series models...
May 22, 2017: Multivariate Behavioral Research
https://www.readbyqxmd.com/read/28532009/exploring-recent-developments-to-improve-antioxidant-anti-inflammatory-and-antimicrobial-efficacy-of-curcumin-a-review-of-new-trends-and-future-perspectives
#17
REVIEW
Zahid Hussain, Hnin Ei Thu, Muhammad Wahab Amjad, Fahad Hussain, Tarek A Ahmed, Shahzeb Khan
Curcumin derivatives have been well-documented due to their natural antioxidant, antimicrobial and anti-inflammatory activities. Curcuminoids have also gained widespread recognition due to their wide range of other activities which include anti-infective, anti-mutagenic, anticancer, anti-coagulant, antiarthrititc, and wound healing potential. Despite of having a wide range of activities, the inherent physicochemical characteristics (poor water solubility, low bioavailability, chemical instability, photodegradation, rapid metabolism and short half-life) of curcumin derivatives limit their pharmaceutical significance...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531172/experimental-evaluation-of-cryopreservative-solutions-to-maintain-in-vitro-and-in-vivo-infectivity-of-p-berghei-sporozoites
#18
Naresh Singh, Samantha J Barnes, Sandra Kennedy, John H Adams
The rodent malaria parasite Plasmodium berghei is an excellent model organism for laboratory-based experimental evaluation of anti-malarial therapeutics prior to studies with human malaria parasites. The rodent model is especially important for evaluation of pre-erythrocytic (PE) stage therapies, especially as current efforts to develop new PE vaccines and drugs is limited by access to P. falciparum and P. vivax sporozoites. Developing a more effective method for cryopreservation of sporozoites would help improve access to sporozoites for laboratories lacking suitable insectary facilities...
2017: PloS One
https://www.readbyqxmd.com/read/28528134/enhancing-tumor-cell-response-to-multidrug-resistance-with-ph-sensitive-quercetin-and-doxorubicin-conjugated-multifunctional-nanoparticles
#19
Cenk Daglioglu
Classical chemotherapy uses chemotherapeutic agents as a mainstay of anticancer treatment. However, the development of multidrug resistance to chemotherapy limits the effectiveness of current cancer treatment. Nanosized bioconjugates combining a chemotherapeutic agent with a pharmacological approach may improve the curative effect of chemotherapeutic agents. Herein I addressed this issue by describing the synthesis, and testing of, pH-responsive Fe3O4@SiO2(FITC)-BTN/QUR/DOX multifunctional nanoparticles. The particles were designed to modulate resistance-mediating factors and to potentiate the efficacy of DOX against chemoresistance...
May 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28528117/composite-elastomeric-polyurethane-scaffolds-incorporating-small-intestinal-submucosa-for-soft-tissue-engineering
#20
Lincui Da, Mei Gong, Anjing Chen, Yi Zhang, Yizhou Huang, Zhijun Guo, Shengfu Li, Jesse Li-Ling, Li Zhang, Huiqi Xie
Although soft tissue replacement has been clinically successful in many cases, the corresponding procedure has many limitations including the lack of resilience and mechanical integrity, significant donor-site morbidity, volume loss with time, and fibrous capsular contracture. These disadvantages can be alleviated by utilizing bio-absorbable scaffolds with high resilience and large strain, which are capable of stimulating natural tissue regeneration. Hence, the chemically crosslinked tridimensional scaffolds obtained by incorporating water-based polyurethane (PU) (which was synthesized from polytetramethylene ether glycol, isophorone diisocyanate, and 2,2-bis(hydroxymethyl) butyric acid) into a bioactive extracellular matrix consisting of small intestinal submucosa (SIS) have been tested in this study to develop a new approach for soft tissue engineering...
May 17, 2017: Acta Biomaterialia
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