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https://www.readbyqxmd.com/read/29668079/co-culture-of-adipose-derived-stem-cells-and-chondrocytes-on-three-dimensionally-printed-bioscaffolds-for-craniofacial-cartilage-engineering
#1
Robert J Morrison, Hassan B Nasser, Khaled N Kashlan, David A Zopf, Derek J Milner, Colleen L Flanangan, Matthew B Wheeler, Glenn E Green, Scott J Hollister
OBJECTIVES/HYPOTHESIS: Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered craniofacial cartilage holds immense potential to surpass current reconstructive options, but limitations to clinical translation exist. We endeavored to determine the viability of utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing to produce 3D bioscaffolds for cartilage tissue engineering...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667084/investigation-of-the-aryl-hydrocarbon-receptor-and-the-intrinsic-tumoral-component-of-the-kynurenine-pathway-of-tryptophan-metabolism-in-primary-brain-tumors
#2
Anthony R Guastella, Sharon K Michelhaugh, Neil V Klinger, Hassan A Fadel, Sam Kiousis, Rouba Ali-Fehmi, William J Kupsky, Csaba Juhász, Sandeep Mittal
INTRODUCTION: There is mounting evidence supporting the role of tryptophan metabolism via the kynurenine pathway (KP) in the pathogenesis of primary brain tumors. Under normal physiological conditions, the KP is the major catabolic pathway for the essential amino acid tryptophan. However, in cancer cells, the KP becomes dysregulated, depletes local tryptophan, and contributes to an immunosuppressive tumor microenvironment. METHODS: We examined the protein expression levels (in 73 gliomas and 48 meningiomas) of the KP rate-limiting enzymes indoleamine 2,3-dioxygenase (IDO) 1, IDO2, and tryptophan 2,3-dioxygenase (TDO2), as well as, the aryl hydrocarbon receptor (AhR), a carcinogenic transcription factor activated by KP metabolites...
April 17, 2018: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29665503/zebrafish-extracellular-matrix-improves-neuronal-viability-and-network-formation-in-a-3-dimensional-culture
#3
Sung-Min Kim, Daniel Ward Long, Michael Wai Kok Tsang, Yadong Wang
Mammalian central nervous system (CNS) has limited capacity for regeneration. CNS injuries cause life-long debilitation and lead to $50 billion in healthcare costs in U.S. alone each year. Despite numerous efforts in the last few decades, CNS-related injuries remain as detrimental as they were 50 years ago. Some functional recovery can occur, but most regeneration are limited by an extracellular matrix (ECM) that actively inhibits axonal repair and promotes glial scarring. In most tissues, the ECM is an architectural foundation that plays an active role in supporting cellular development and regenerative response after injury...
April 6, 2018: Biomaterials
https://www.readbyqxmd.com/read/29665365/dehydroeffusol-inhibits-viability-and-epithelial-mesenchymal-transition-through-the-hedgehog-and-akt-mtor-signaling-pathways-in-neuroblastoma-cells
#4
Kang He, Guoqing Duan, Yanyang Li
Neuroblastoma (NB) is the most predominant extracranial solid tumor of infancy in the world. However, current chemotherapy has limited efficacy for more advanced stages of NB due to acquired chemoresistance or acute toxicity in NB patients. Therefore, effective novel anti-NB drugs are desperately needed. The present study aimed to investigate the effects of dehydroeffusol (DHE), a phenanthrene isolated from J. effuses, on NB cells and its underlying mechanism. The results showed that DHE treatment effectively inhibited NB cell viability in a dose-dependent manner...
April 14, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29665026/anhydrous-polymer-based-coating-with-sustainable-controlled-release-functionality-for-facile-efficacious-impregnation-and-delivery-of-antimicrobial-peptides
#5
Kaiyang Lim, Rathi Saravanan, Kian Long Kelvin Chong, Hwee Mian Sharon Goh, Ray Rong Yuan Chua, Paul Anantharajah Tambyah, Matthew Wook Chang, Kimberly A Kline, Susanna Su Jan Leong
Anhydrous polymers are actively explored as alternative materials to overcome limitations of conventional hydrogel-based antibacterial coating. However, the requirement for strong organic solvent in polymerization reactions often necessitates extra protection steps for encapsulation of target biomolecules, lowering encapsulation efficiency and increasing process complexity. This study reports a novel coating strategy that allows direct solvation and encapsulation of antimicrobial peptides (HHC36) into anhydrous polycaprolactone (PCL) polymer-based dual layer coating...
April 17, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29662849/dextran-conjugated-lysozymes-inhibit-the-growth-of-shigella-sonnei-and-viral-hemorrhagic-septicemia-virus
#6
Joongjae Lee, Inyong Kim, Seoungsoon Yeo, Duwoon Kim, Misook Kim
Lysozyme is well known as a natural antimicrobial agent, but its function is limited in that it only combats Gram-positive bacteria. We investigated the inhibitory effects of dextran-conjugated lysozymes (DLs) against some strains of Gram-negative bacteria and viral hemorrhagic septicemia virus (VHSV). The Maillard reactions of the DL were performed at various pHs (3.0, 7.0, and 8.5) in the presence of saturated KBr solution for 1, 3, 5, 7, and 9 days. The growth inhibition effects against Gram-negative bacterial strains, such as Escherichia coli , Vibrio parahaemolyticus , Pseudomonas aeruginosa , Shigella sonnei , and Shigella flexneri were found only in some DLs...
March 2018: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29662625/adam17-inhibition-enhances-platinum-efficiency-in-ovarian-cancer
#7
Nina Hedemann, Christoph Rogmans, Susanne Sebens, Daniela Wesch, Manuel Reichert, Dirk Schmidt-Arras, Hans-Heinrich Oberg, Ulrich Pecks, Marion van Mackelenbergh, Jörg Weimer, Norbert Arnold, Nicolai Maass, Dirk O Bauerschlag
Chemotherapeutic resistance evolves in about 70 % of ovarian cancer patients and is a major cause of death in this tumor entity. Novel approaches to overcome these therapeutic limitations are therefore highly warranted. A disintegrin and metalloprotease 17 (ADAM17) is highly expressed in ovarian cancer and required for releasing epidermal growth factor receptor (EGFR) ligands like amphiregulin (AREG). This factor has recently been detected in ascites of advanced stage ovarian cancer patients. However, it is not well understood, whether and how ADAM17 might contribute to chemo resistance of ovarian cancer...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29661755/a-cd123-targeting-antibody-drug-conjugate-imgn632-designed-to-eradicate-aml-while-sparing-normal-bone-marrow-cells
#8
Yelena Kovtun, Gregory E Jones, Sharlene Adams, Lauren Harvey, Charlene A Audette, Alan Wilhelm, Chen Bai, Lingyun Rui, Rassol Laleau, Fenghua Liu, Olga Ab, Yulius Setiady, Nicholas C Yoder, Victor S Goldmacher, Ravi V J Chari, Jan Pinkas, Thomas Chittenden
The outlook for patients with refractory/relapsed acute myeloid leukemia (AML) remains poor, with conventional chemotherapeutic treatments often associated with unacceptable toxicities, including severe infections due to profound myelosuppression. Thus there exists an urgent need for more effective agents to treat AML that confer high therapeutic indices and favorable tolerability profiles. Because of its high expression on leukemic blast and stem cells compared with normal hematopoietic stem cells and progenitors, CD123 has emerged as a rational candidate for molecularly targeted therapeutic approaches in this disease...
April 24, 2018: Blood Advances
https://www.readbyqxmd.com/read/29660693/the-partial-root-zone-saline-irrigation-system-and-antioxidant-responses-in-tomato-plants
#9
Rita de Cássia Alves, Ana Santana de Medeiros, Mayara Cristina Malvas Nicolau, Antônio Pizolato Neto, Francisco de Assis Oliveira, Leonardo Warzea Lima, Tiago Tezotto, Priscila Lupino Gratão
Salinity is a limiting factor that can affect plant growth and cause significant losses in agricultural productivity. This study provides an insight about the viability of partial root-zone irrigation (PRI) system with saline water supported by a biochemical approach involving antioxidant responses. Six different irrigation methods using low and high salt concentrations (S1-0.5 and S2-5.0 dS m-1 ) were applied, with or without PRSI, so that one side of the root-zone was submitted to saline water while the other side was low salinity water irrigated...
April 6, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29660518/hoxb4-knockdown-enhances-the-cytotoxic-effect-of-paclitaxel-and-cisplatin-by-downregulating-abc-transporters-in-ovarian-cancer-cells
#10
Ying Li, Jingli Sun, Shaofeng Gao, Heping Hu, Pengmu Xie
Therapeutic effects of anti-cancer drugs for ovarian cancer were limited due to the rapid development of chemotherapy resistance. The aim of this study was to test whether knockdown of Homeobox B4 (HOXB4) enhanced the cytotoxic effect of paclitaxel and cisplatin in ovarian cancer cells. HOXB4 expressions at mRNA and protein levels were upregulated in Taxol-resistant A2780 (A2780/Taxol) and DDP-resistant SKOV-3 (SKOV-3/DDP) cells. HOXB4 knockdown enhanced the cytotoxic effects of Taxol and DDP in A2780/Taxol and SKOV-3/DDP cells, respectively...
April 13, 2018: Gene
https://www.readbyqxmd.com/read/29660265/hypochlorite-mediated-modulation-of-photo-induced-electron-transfer-in-ptz-bodipy-electron-donor-acceptor-dyad-a-highly-water-soluble-turn-on-fluorescent-probe-for-hypochlorite
#11
Raghu Chitta, Disha Soni, Naresh Duvva, Deepak Badgurjar, Tapta Kanchan Roy, Surendra Nimesh, Geeta Arya, Lingamallu Giribabu
A highly water soluble phenothiazine (PTZ)-borondipyrromethene (BODIPY) based electron donor-acceptor dyad, WS-Probe, containing BODIPY as the signaling antennae and PTZ as the OCl- reactive group was designed and used as a fluorescent chemosensor for the detection of OCl-. Upon addition of incremental amounts of NaOCl, the quenched fluorescence of WS-Probe was enhanced drastically, indicating the inhibition of reductive photo-induced electron transfer (PET) from PTZ to 1BODIPY* and the detection limit was calculated to be 26...
April 16, 2018: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/29660027/the-6-hydroxychromanol-derivative-sul-109-ameliorates-renal-injury-after-deep-hypothermia-and-rewarming-in-rats
#12
Pieter C Vogelaar, Maurits Roorda, Edwin L de Vrij, Martin C Houwertjes, Maaike Goris, Hjalmar Bouma, Adrianus C van der Graaf, Guido Krenning, Robert H Henning
Background: Mitochondrial dysfunction plays an important role in kidney damage in various pathologies, including acute and chronic kidney injury and diabetic nephropathy. In addition to the well-studied ischaemia/reperfusion (I/R) injury, hypothermia/rewarming (H/R) also inflicts acute kidney injury. Substituted 6-hydroxychromanols are a novel class of mitochondrial medicines that ameliorate mitochondrial oxidative stress and protect the mitochondrial network. To identify a novel 6-hydroxychromanol that protects mitochondrial structure and function in the kidney during H/R, we screened multiple compounds in vitro and subsequently assessed the efficacy of the 6-hydroxychromanol derivatives SUL-109 and SUL-121 in vivo to protect against kidney injury after H/R in rats...
April 11, 2018: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29659619/characterisation-of-two-toxoplasma-proppins-homologous-to-atg18-wipi-suggests-they-have-evolved-distinct-specialised-functions
#13
Hoa Mai Nguyen, Shuxian Liu, Wassim Daher, Feng Tan, Sébastien Besteiro
Toxoplasma gondii is a parasitic protist possessing a limited set of proteins involved in the autophagy pathway, a self-degradative machinery for protein and organelle recycling. This distant eukaryote has even repurposed part of this machinery, centered on protein ATG8, for a non-degradative function related to the maintenance of the apicoplast, a parasite-specific organelle. However, some evidence also suggest Toxoplasma is able to generate autophagic vesicles upon stress, and that some autophagy-related proteins, such as ATG9, might be involved solely in the canonical autophagy function...
2018: PloS One
https://www.readbyqxmd.com/read/29659243/carbonization-of-human-fingernails-towards-the-sustainable-production-of-multifunctional-nitrogen-and-sulfur-co-doped-carbon-nanodots-with-highly-luminescent-probing-and-cell-proliferative-migration-properties
#14
Theodoros G Chatzimitakos, Athanasia Kasouni, Anastassios N Troganis, Constantine D Stalikas
A simple, yet effective method is employed to prepare multifunctional fluorescent carbon nanodots (CNDs) from human fingernails. The results demonstrate that the CNDs have excellent optical properties and a quantum yield of 81%, which is attributed to the intrinsic composition of precursor material itself. The CNDs are used to develop an ultra-sensitive fluorescent probe for the detection of hexavalent chromium (limit of detection: 0.3 nM) via a combined inner- filter and static mechanism. Moreover, the toxicity of the CNDs over four epithelial cell lines is assessed...
April 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29656034/breastmilk-donations-bacteriological-assessment-analysis-of-causes-of-non-compliance-and-suggestions-for-improvement
#15
C Mullié, O Obin, G Outurquin, S Grognet, A Léké, C Adjidé
A total of 1099 breastmilk donations received by the milk bank at the Amiens University Hospital from January to June 2016 were assessed for bacteriological contamination according to French regulations. This consisted in enumerating the total aerobic flora before and after heat treatment as well as the specific enumeration of coagulase-positive staphylococci. Results above the mandatory limits for at least one of these parameters were found in 25.9% of the donations, resulting in the destruction of approximately one-quarter of the volume of the donations (∼195L)...
April 11, 2018: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/29651966/specific-cytostatic-and-cytotoxic-effect-of-dihydrochelerythrine-in-glioblastoma-cells-role-of-nf-kb-b-catenin-and-stat3-il-6-pathways
#16
Tereza Cristina Costa Silva, Noelio de Jesus Menezes-Filho, Giselle Pinto de Faria Lopes, Diego Madureira de Oliveira, Ezequiel Pereira, Bruno Penas Seara Pitanga, Eudes S Velozo, Songeli Menezes Freire, Jorge Clarencio de Souza Andrade, Helena Lobo Borges, Stevens Kastrup Rehen, Vivaldo Moura-Neto, Silvia Lima Costa
A glioblastoma is a primary CNS tumor that is more aggressive and lethal than other brain tumors. Its location, rapid proliferation, invasive growth, angiogenesis and immunosuppression are the main factors that limit its treatment, making it a major challenge to neuro-oncology. OBJECTIVE: This study investigated the in vitro effects of the alkaloid dihydrochelerythrine (DHC), which is extracted from Zanthoxylum stelligerum, on the viability, proliferation, cell death and b-catenin, NFkB, STAT3/pSTAT3 and interleukins roles...
April 12, 2018: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29645346/identification-of-a-siglec-f-granulocyte-macrophage-progenitor
#17
Jessica E Bolden, Erin C Lucas, Geyu Zhou, Jeremy A O'Sullivan, Carolyn A de Graaf, Mark D McKenzie, Ladina Di Rago, Tracey M Baldwin, Jake Shortt, Warren S Alexander, Bruce S Bochner, Matthew E Ritchie, Douglas J Hilton, Kirsten A Fairfax
In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils...
April 12, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29642963/nanoencapsulation-of-benznidazole-in-calcium-carbonate-increases-its-selectivity-to-trypanosoma-cruzi
#18
Louise Donadello Tessarolo, Ramon Róseo Paula Pessoa Bezerra de Menezes, Clarissa Perdigão Mello, Dânya Bandeira Lima, Emanuel Paula Magalhães, Eveline Matias Bezerra, Francisco Adilson Matos Sales, Ito Liberato Barroso Neto, Maria de Fátima Oliveira, Ricardo Pires Dos Santos, Eudenilson L Albuquerque, Valder Nogueira Freire, Alice Maria Martins
Chagas disease is a public health problem, affecting about 7 million people worldwide. Benznidazole (BZN) is the main treatment option, but it has limited effectiveness and can cause severe adverse effects. Drug delivery through nanoparticles has attracted the interest of the scientific community aiming to improve therapeutic options. The aim of this study was to evaluate the cytotoxicity of benznidazole-loaded calcium carbonate nanoparticles (BZN@CaCO3) on Trypanosoma cruzi strain Y. It was observed that BZN@CaCO3 was able to reduce the viability of epimastigote, trypomastigote and amastigote forms of T...
April 12, 2018: Parasitology
https://www.readbyqxmd.com/read/29642830/-a-new-strategy-for-enhancing-acanthamoebicidal-activity-with-synthesis-of-nanoflower-of-laurocerausus-officinalis-roemer-cherry-laurel-fruit-extracts
#19
Ayşe Baldemir, Ülkü Karaman, Sadi Yusufbeyoğlu, Ayşe Eken, Nilay Ildız, Selen İlgün, Cemil Çolak, Gamze Kaçmaz, İsmail Öçsoy, Soner Çankaya
Pathogenic Acanthamoeba species often cause infection known as Acanthamoeba keratitis among people who use contact lenses. It is a type of infection that can result in corneal ulceration, visual loss or even blindness, if not treated. There are various therapeutic options available in the treatment of Acanthamoeba infections but they are usually tough treatments with limited efficacy. For instance, hydrogen peroxide (H2 O2 ) is a commonly used contact lens disinfectant which is effective against Acanthamoeba but it is toxic to the cornea...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29642388/oligodendroglioma-cells-lack-glutamine-synthetase-and-are-auxotrophic-for-glutamine-but-do-not-depend-on-glutamine-anaplerosis-for-growth
#20
Martina Chiu, Giuseppe Taurino, Massimiliano G Bianchi, Laura Ottaviani, Roberta Andreoli, Tecla Ciociola, Costanza A M Lagrasta, Saverio Tardito, Ovidio Bussolati
In cells derived from several types of cancer, a transcriptional program drives high consumption of glutamine (Gln), which is used for anaplerosis, leading to a metabolic addiction for the amino acid. Low or absent expression of Glutamine Synthetase (GS), the only enzyme that catalyzes de novo Gln synthesis, has been considered a marker of Gln-addicted cancers. In this study, two human cell lines derived from brain tumors with oligodendroglioma features, HOG and Hs683, have been shown to be GS-negative. Viability of both lines depends from extracellular Gln with EC50 of 0...
April 6, 2018: International Journal of Molecular Sciences
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