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https://www.readbyqxmd.com/read/28212822/cryptosporidium-contaminated-water-disinfection-by-a-novel-fenton-process
#1
Sina Matavos-Aramyan, Mohsen Moussavi, Hedieh Matavos-Aramyan, Sara Roozkhosh
Three novel modified advanced oxidation process systems including ascorbic acid-, pro-oxidants- and ascorbic acid-pro-oxidants-modified Fenton system were utilized to study the disinfection efficiency on Cryptosporidium-contaminated drinking water samples. Different concentrations of divalent and trivalent iron ions, hydrogen peroxide, ascorbic acid and pro-oxidants at different exposure times were investigated. These novel systems were also compared to the classic Fenton system and to the control system which comprised of only hydrogen peroxide...
February 16, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28211933/enhanced-bone-tissue-regeneration-using-a-3d-printed-microstructure-incorporated-with-a-hybrid-nano-hydrogel
#2
Dong Nyoung Heo, Nathan J Castro, Se-Jun Lee, Hanaul Noh, Wei Zhu, Lijie Grace Zhang
Three-dimensional (3D) functional constructs with biomimetic mechanical and chemical properties are ideal for various regenerative medicine applications. These properties of 3D fabricated constructs mainly depend on the intrinsic characteristics of the materials and fabrication method. In this respect, the current use of hydrogels for musculoskeletal tissue repair is not ideal due to the lack of suitable mechanical properties, as well as the high biomimetic requirement for success. To overcome this limitation, we developed a novel functionalized hydrogel with bioactive gold nanoparticles (GNPs), reinforcing a 3D printed microstructure via fused deposition modeling (FDM) for bone tissue regeneration...
February 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28210981/assessing-common-bean-cultivars-for-resistance-to-the-soybean-looper-chrysodeixis-includens-lepidoptera-noctuidae
#3
R Morando, E L L Baldin, P L Cruz, A L Lourenção
The soybean looper Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae) is known as an important pest of leguminous plants worldwide. In Brazil, this pest species is gaining importance to producers of the common bean Phaseolus vulgaris L. (Fabaceae) because it limits field production of the crop. Chemical control is still the primary method of insect control. However, due to the possible harmful effects of pesticides to humans and the environment, alternative and less aggressive practices are being investigated...
February 16, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28210432/biocompatibility-of-mineral-trioxide-aggregate-with-tio2-nanoparticles-on-human-gingival-fibroblasts
#4
Mohammad Samiei, Negin Ghasemi, Marzieh Aghazadeh, Baharak Divband, Farzaneh Akbarzadeh
BACKGROUND: The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA's ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at different storage times after mixing on human gingival fibroblasts (HGFs). MATERIAL AND METHODS: HGFs were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco's Modified Eagle medium, supplemented with 10% fetal calf serum, penicillin and streptomycin...
February 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28210254/influence-of-oxidative-stress-on-biocontrol-activity-of-cryptococcus-laurentii-against-blue-mold-on-peach-fruit
#5
Zhanquan Zhang, Jian Chen, Boqiang Li, Chang He, Yong Chen, Shiping Tian
The limitations of chemical fungicides for the control of postharvest diseases have recently become more apparent. The utilization of antagonistic microorganisms is a promising alternative to that of fungicides to control postharvest decay. In previous studies, the antagonistic yeast Cryptococcus laurentii has shown excellent effects of biocontrol and great potential for practical application. Adverse conditions, such as oxidative stress, limit the practical application of antagonistic yeast. In this study, we investigated the oxidative stress tolerance of C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28209403/small-molecules-that-sabotage-bacterial-virulence
#6
REVIEW
Benjamin K Johnson, Robert B Abramovitch
The continued rise of antibiotic-resistant bacterial infections has motivated alternative strategies for target discovery and treatment of infections. Antivirulence therapies function through inhibition of in vivo required virulence factors to disarm the pathogen instead of directly targeting viability or growth. This approach to treating bacteria-mediated diseases may have advantages over traditional antibiotics because it targets factors specific for pathogenesis, potentially reducing selection for resistance and limiting collateral damage to the resident microbiota...
February 13, 2017: Trends in Pharmacological Sciences
https://www.readbyqxmd.com/read/28207788/self-complementary-adeno-associated-virus-serotype-6-mediated-knockdown-of-adamts4-induces-long-term-and-effective-enhancement-of-aggrecan-in-degenerative-human-nucleus-pulposus-cells-a-new-therapeutic-approach-for-intervertebral-disc-disorders
#7
Demissew Shenegelegn Mern, Anja Tschugg, Sebastian Hartmann, Claudius Thomé
Inhibition of intervertebral disc (IVD) degeneration, which is often accompanied by painful inflammatory and immunopathological processes, is challenging. Current IVD gene therapeutic approaches are based on adenoviral gene delivery systems, which are limited by immune reactions to their viral proteins. Their applications in IVDs near to sensitive neural structure could provoke toxicity and immunological side-effects with neurological deficits. Self-complementary adeno-associated virus (scAAV) vectors, which do not express any viral gene and are not linked with any known disease in humans, are attractive therapeutic gene delivery vectors in degenerative IVDs...
2017: PloS One
https://www.readbyqxmd.com/read/28206821/simulated-space-radiation-impact-of-four-different-types-of-high-dose-ionizing-radiation-on-the-lichen-xanthoria-elegans
#8
Annette Brandt, Joachim Meeßen, Reiner U Jänicke, Marina Raguse, Sieglinde Ott
This study addresses the viability of the lichen Xanthoria elegans after high-dose ionizing irradiation in the frame of the STARLIFE campaign. The first set of experiments was intended to resemble several types of galactic cosmic radiation (GCR) as present beyond the magnetic shield of Earth. In the second set of experiments, γ radiation up to 113 kGy was applied to test the limit of lichen resistance to ionizing radiation. Entire thalli of Xanthoria elegans were irradiated in the anhydrobiotic state. After STARLIFE 1, the metabolic activity of both symbionts was quantified by live/dead staining with confocal laser scanning microscopy...
February 2017: Astrobiology
https://www.readbyqxmd.com/read/28202079/a-novel-cytoprotective-peptide-protects-mesenchymal-stem-cells-against-mitochondrial-dysfunction-and-apoptosis-induced-by-starvation-via-nrf2-sirt3-foxo3a-pathway
#9
Shuo Wang, Chao Zhang, Sidikejiang Niyazi, Long Zheng, Jiawei Li, Weitao Zhang, Ming Xu, Ruiming Rong, Cheng Yang, Tongyu Zhu
BACKGROUND: Mesenchymal stem cell (MSC) has been widely explored in the past decade as a cell-based treatment for various diseases. However, poor survival of adaptively transferred MSCs limits their clinical therapeutic potentials, which is largely ascribed to the nutrient starvation. In this study, we determined whether a novel kidney protective peptide CHBP could protect MSCs against starvation and invested the underlying mechanisms. METHODS: MSCs were subjected to serum deprivation and CHBP of graded concentrations was administered...
February 15, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28202053/up-regulation-of-mir-146a-increases-the-sensitivity-of-non-small-cell-lung-cancer-to-ddp-by-downregulating-cyclin-j
#10
Lin Shi, Zhaozhong Xu, Gang Wu, Xiaoting Chen, Yuanyuan Huang, Yanjing Wang, Weiqiang Jiang, Bin Ke
BACKGROUND: Cisplatin (DDP)-based chemotherapy is the common first-line therapy for lung cancer. However, their efficacy is often limited by primary drug resistance and/or acquired drug resistance. The aim of this study was to investigate the function of miRNA-146a (miR-146a) in DDP-resistant non-small cell lung cancer (NSCLC), as well as the underlying mechanisms. METHODS: The effect of overexpression of miR-146a and/or knockdown of cyclin J (CCNJ) in A549/DDP and SPC-A1/DDP cells were investigated as follows...
February 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28195888/a-novel-application-of-double-paddle-peroneal-chimeric-flap-as-external-sentinel-monitor-in-hypopharyngeal-reconstruction
#11
Jung-Hua Hsueh, Yu-Hsuan Hsieh, Ying-Sheng Lin, Yaoh-Shiang Lin, Kuo-Chung Yang, Wen-Chung Liu
OBJECTIVES: Monitoring the viability of buried free flaps in hypopharyngeal reconstruction is difficult. Various methods have been proposed to monitor these buried flaps, but limitations remain. METHOD: We present a method of using double-paddle chimeric peroneal flaps that permanently externalizes a flap skin paddle as a monitoring paddle in hypopharyngeal reconstructions. This study was performed in a medical center in southern Taiwan; between 2013 and 2015, 10 patients underwent double-paddle chimeric peroneal flap reconstruction in advanced stages of hypopharyngeal cancer...
February 14, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28193999/human-skeletal-muscle-plasmalemma-alters-its-structure-to-change-its-ca-2-handling-following-heavy-load-resistance-exercise
#12
Tanya R Cully, Robyn M Murphy, Llion Roberts, Truls Raastad, Robert G Fassett, Jeff S Coombes, Isuru D Jayasinghe, Bradley S Launikonis
High-force eccentric exercise results in sustained increases in cytoplasmic Ca(2+) levels ([Ca(2+)]cyto), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system, causing the loss of its predominantly transverse organization and an increase in vacuolation of its longitudinal tubules across adjacent sarcomeres. The transverse tubules and vacuoles displayed distinct Ca(2+)-handling properties...
February 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28193542/sulfated-hyaluronic-acid-hydrogels-with-retarded-degradation-and-enhanced-growth-factor-retention-promote-hmsc-chondrogenesis-and-articular-cartilage-integrity-with-reduced-hypertrophy
#13
Qian Feng, Sien Lin, Kunyu Zhang, Chaoqun Dong, Tianyi Wu, Heqin Huang, Xiaohui Yan, Li Zhang, Gang Li, Liming Bian
: Recently, hyaluronic acid (HA) hydrogels have been extensively researched for delivering cells and drugs to repair damaged tissues, particularly articular cartilage. However, the in vivo degradation of HA is fast, thus limiting the clinical translation of HA hydrogels. Furthermore, HA cannot bind proteins with high affinity because of the lack of negatively charged sulfate groups. In this study, we conjugated sulfate groups to HA. These sulfated HA exhibit significantly slower degradation by hyaluronidase compared to the wild type HA...
February 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28191587/cytotoxic-responses-of-carnosic-acid-and-doxorubicin-on-breast-cancer-cells-in-butterfly-shaped-microchips-in-comparison-to-2d-and-3d-culture
#14
Ece Yildiz-Ozturk, Sultan Gulce-Iz, Muge Anil, Ozlem Yesil-Celiktas
Two dimensional (2D) cell culture systems lack the ability to mimic in vivo conditions resulting in limitations for preclinical cell-based drug and toxicity screening assays and modelling tumor biology. Alternatively, 3D cell culture systems mimic the specificity of native tissue with better physiological integrity. In this regard, microfluidic chips have gained wide applicability for in vitro 3D cancer cell studies. The aim of this research was to develop a 3D biomimetic model comprising culture of breast cancer cells in butterfly-shaped microchip to determine the cytotoxicity of carnosic acid and doxorubicin on both estrogen dependent (MCF-7) and independent (MDA-MB231) breast cancer cells along with healthy mammary epithelial cells (MCF-10A) in 2D, 3D Matrigel™ and butterfly-shaped microchip environment...
February 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28188686/toxic-effects-of-tio2-nanoparticles-in-primary-cultured-rat-sertoli-cells-are-mediated-via-a-dysregulated-ca-2-pkc-p38-mapk-nf-%C3%AE%C2%BAb-cascade
#15
Lingqun Ye, Fashui Hong, Xiao Ze, Lingjuan Li, Yaoming Zhou, Yuguan Ze
Although numerous studies have demonstrated that titanium dioxide nanoparticles (TiO2 NPs) can be accumulated in various animal organs and can cause toxicity, there is currently only limited data regarding reproductive toxicity especially on the toxic mechanisms of TiO2 NPs in Sertoli cells. In order to investigate the mechanism of reproductive toxicity, primary cultured rat Sertoli cells were exposed to 5, 15 or 30 μg/ml TiO2 NPs for 24 h, and TiO2 NPs internalization, expression of PKC (p-PKC) and p38 MAPK (p-p38 MAPK) as well as calcium homeostasis were examined...
February 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28188071/a-good-manufacturing-practice-grade-standard-protocol-for-exclusively-human-mesenchymal-stromal-cell-derived-extracellular-vesicles
#16
Karin Pachler, Thomas Lener, Doris Streif, Zsuzsanna A Dunai, Alexandre Desgeorges, Martina Feichtner, Michaela Öller, Katharina Schallmoser, Eva Rohde, Mario Gimona
BACKGROUND AIMS: Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) may contribute to biological processes such as tissue regeneration, immunomodulation and neuroprotection. Evaluation of their therapeutic potential and application in future clinical trials demands thorough characterization of EV content and production under defined medium conditions, devoid of xenogenic substances and serum-derived vesicles. Addressing the apparent need for such a growth medium, we have developed a medium formulation based on pooled human platelet lysate (pHPL), free from animal-derived xenogenic additives and depleted of EVs...
February 7, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28187326/effect-of-chitosan-and-so2-on-viability-of-acetobacter-strains-in-wine
#17
Maria José Valera, Florencia Sainz, Albert Mas, María Jesús Torija
Wine spoilage is an important concern for winemakers to preserve the quality of their final product and avoid contamination throughout the production process. The use of sulphur dioxide (SO2) is highly recommended to prevent wine spoilage due to its antimicrobial activity. However, SO2 has a limited effect on the viability of acetic acid bacteria (AAB). Currently, the use of SO2 alternatives is favoured in order to reduce the use of chemicals and improve stabilization in winemaking. Chitosan is a biopolymer that is approved by the European authorities and the International Organization of Vine and Wine to be used as a fining agent and antimicrobial in wines...
February 1, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28187190/widespread-gli-expression-but-limited-canonical-hedgehog-signaling-restricted-to-the-ductular-reaction-in-human-chronic-liver-disease
#18
Candice Alexandra Grzelak, Nicholas David Sigglekow, Janina Elke Eleonore Tirnitz-Parker, Elizabeth Jane Hamson, Alessandra Warren, Bharvi Maneck, Jinbiao Chen, Bramilla Patkunanathan, Jade Boland, Robert Cheng, Nicholas Adam Shackel, Devanshi Seth, David Geoffrey Bowen, Luciano Gastón Martelotto, D Neil Watkins, Geoffrey William McCaughan
Canonical Hedgehog (Hh) signaling in vertebrate cells occurs following Smoothened activation/translocation into the primary cilia (Pc), followed by a GLI transcriptional response. Nonetheless, GLI activation can occur independently of the canonical Hh pathway. Using a murine model of liver injury, we previously identified the importance of canonical Hh signaling within the Pc+ liver progenitor cell (LPC) population and noted that SMO-independent, GLI-mediated signals were important in multiple Pc-ve GLI2+ intrahepatic populations...
2017: PloS One
https://www.readbyqxmd.com/read/28186983/differentiation-status-of-primary-chronic-myeloid-leukemia-cells-affects-sensitivity-to-bcr-abl1-inhibitors
#19
Paavo O Pietarinen, Christopher A Eide, Pilar Ayuda-Durán, Swapnil Potdar, Heikki Kuusanmäki, Emma I Andersson, John P Mpindi, Tea Pemovska, Mika Kontro, Caroline A Heckman, Olli Kallioniemi, Krister Wennerberg, Henrik Hjorth-Hansen, Brian J Druker, Jorrit M Enserink, Jeffrey W Tyner, Satu Mustjoki, Kimmo Porkka
Tyrosine kinase inhibitors (TKI) are the mainstay treatment of BCR-ABL1-positive leukemia and virtually all patients with chronic myeloid leukemia in chronic phase (CP CML) respond to TKI therapy. However, there is limited information on the cellular mechanisms of response and particularly on the effect of cell differentiation state to TKI sensitivity in vivo and ex vivo/in vitro. We used multiple, independent high-throughput drug sensitivity and resistance testing platforms that collectively evaluated 295 oncology compounds to characterize ex vivo drug response profiles of primary cells freshly collected from newly-diagnosed patients with BCR-ABL1-positive leukemia (n = 40) and healthy controls (n = 12)...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28184014/autophagy-inhibition-enhances-sunitinib-efficacy-in-clear-cell-ovarian-carcinoma
#20
Lindsay DeVorkin, Matthew Hattersley, Paul Kim, Jenna Ries, Jaeline Spowart, Michael S Anglesio, Samuel M Levi, David G Huntsman, Ravi K Amaravadi, Jeffrey D Winkler, Anna V Tinker, Julian J Lum
Clear cell ovarian carcinoma (CCOC) is an aggressive form of epithelial ovarian cancer that exhibits low response rates to systemic therapy and poor patient outcomes. Multiple studies in CCOC have revealed expression profiles consistent with increased hypoxia, and our previous data suggest that hypoxia is correlated with increased autophagy in CCOC. Hypoxia-induced autophagy is a key factor promoting tumor cell survival and resistance to therapy. Recent clinical trials with the molecular-targeted receptor tyrosine kinase (RTK) inhibitor sunitinib have demonstrated limited activity...
February 9, 2017: Molecular Cancer Research: MCR
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