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https://www.readbyqxmd.com/read/29028843/efficiency-of-different-air-filter-types-for-pig-facilities-at-laboratory-scale
#1
Cindy Wenke, Janina Pospiech, Tobias Reutter, Uwe Truyen, Stephanie Speck
Air filtration has been shown to be efficient in reducing pathogen burden in circulating air. We determined at laboratory scale the retention efficiency of different air filter types either composed of a prefilter (EU class G4) and a secondary fiberglass filter (EU class F9) or consisting of a filter mat (EU class M6 and F8-9). Four filter prototypes were tested for their capability to remove aerosol containing equine arteritis virus (EAV), porcine reproductive and respiratory syndrome virus (PRRSV), bovine enterovirus 1 (BEV), Actinobacillus pleuropneumoniae (APP), and Staphylococcus (S...
2017: PloS One
https://www.readbyqxmd.com/read/29027187/midterm-results-of-trochanteric-flip-osteotomy-technique-for-management-of-fractures-around-the-hip
#2
Vivek Trikha, Saubhik Das, Arkesh Madegowda, Prabhat Agrawal
INTRODUCTION: In this study, we aimed to investigate safety and efficacy of the trochanteric flip osteotomy with surgical hip dislocation technique in selected displaced acetabular and femoral head fractures with clinico-radiological outcome and potential complications. MATERIALS AND METHODS: We retrospectively reviewed 32 patients from January 2009 to June 2014. Selected displaced acetabular fractures with comminution and/or cranial extension of posterior wall, marginal impaction, intraarticular fragment, femoral head fractures and hip fracture-dislocations were operated by this modified approach of trochanteric flip osteotomy and surgical hip dislocation...
October 9, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29025637/electrospun-biphasic-tubular-scaffold-with-enhanced-mechanical-properties-for-vascular-tissue-engineering
#3
Abdalla Abdal-Hay, Michal Bartnikowski, Stephen Hamlet, Sašo Ivanovski
Polymer scaffolds produced through an electrospinning process are frequently explored as tissue substitutes for regenerative medicine. Despite offering desirable surface area to volume ratios and tailorable pore sizes, their poor structural mechanical properties limit their applicability in load-bearing regions. In this study, we present a simple strategy to improve the mechanical properties of a vascular graft scaffold. We achieved the formation of biphasic tubular scaffolds by electrospinning polyurethane (PU) onto an airbrushed tube made of polycaprolactone (PCL)...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29024547/equine-endometrial-explants-undergo-significant-degenerative-changes-in-culture
#4
Rose Schwinghamer, Alessandro Massolo, Cameron Knight, Claudia Klein
The current study evaluated equine endometrial explants following 12, 24, and 48 hours in culture. Measurement of an indicator of cell death in explant supernatant, light microscopy, and gene expression of biomarkers of endometrial function and cellular stress, were used to compare the effect of six different media on explant viability and morphology. Viability of explants was assessed indirectly through measuring LDH activity in the culture supernatant. Regardless of culture medium composition, a significant increase in LDH activity was observed within 12 hours of culture, indicating occurrence of cell damage...
October 12, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29023454/antifungal-defense-of-probiotic-lactobacillus-rhamnosus-gg-is-mediated-by-blocking-adhesion-and-nutrient-depletion
#5
Daniela Mailänder-Sánchez, Christina Braunsdorf, Christian Grumaz, Christoph Müller, Stefan Lorenz, Philip Stevens, Jeanette Wagener, Betty Hebecker, Bernhard Hube, Franz Bracher, Kai Sohn, Martin Schaller
Candida albicans is an inhabitant of mucosal surfaces in healthy individuals but also the most common cause of fungal nosocomial blood stream infections, associated with high morbidity and mortality. As such life-threatening infections often disseminate from superficial mucosal infections we aimed to study the use of probiotic Lactobacillus rhamnosus GG (LGG) in prevention of mucosal C. albicans infections. Here, we demonstrate that LGG protects oral epithelial tissue from damage caused by C. albicans in our in vitro model of oral candidiasis...
2017: PloS One
https://www.readbyqxmd.com/read/29022506/autologous-cell-therapy-for-vascular-regeneration-the-role-of-proangiogenic-cells
#6
Vanessa Bianconi, Francesca Fallarino, Massimo Raffaele Mannarino, Francesco Bagaglia, Meisam Naeimi Kararoudi, Caterina Oriana Aragona, Rita Romani, Matteo Pirro
Autologous cell therapy represents a novel treatment option for vascular regeneration in different disease conditions. Both experimental and clinical studies indicate a therapeutic potential for different proangiogenic cells (PCs), including endothelial progenitor cells (EPCs) in the treatment of peripheral artery disease. A significant improvement of ischemic symptoms has been reported in different trials evaluating PCs for the treatment of critical limb ischemia. However, contrasting results from different meta-analyses question the efficacy of PC-based approaches on long-term outcomes, including amputation and survival...
October 12, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29022483/in-vitro-combined-effect-of-doxorubicin-and-sulfonated-zinc-phthalocyanine-mediated-photodynamic-therapy-on-mcf-7-breast-cancer-cells
#7
Eric Chekwube Aniogo, Blassan Plackal Adimuriyil George, Heidi Abrahamse
Doxorubicin is a broad-spectrum antibiotic and anticancer drug used to treat a variety of human malignancies like breast cancer and leukaemia. Unfortunately, a dose-dependent side effect of this drug is common, representing a major obstacle to its use despite its therapeutic efficacy. Photodynamic therapy is an emerging non-invasive potential adjuvant for conventional cancer treatment. In an attempt to circumvent the dose-limiting effect of doxorubicin, this study aimed to investigate cellular anticancer activity of doxorubicin and sulfonated zinc phthalocyanine-mediated photodynamic therapy on MCF-7 cells alone and in combination...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29020015/anti-inflammatory-and-wound-healing-activities-of-calophyllolide-isolated-from-calophyllum-inophyllum-linn
#8
Van-Linh Nguyen, Cong-Tri Truong, Binh Cao Quan Nguyen, Thanh-Niem Van Vo, Trong-Thuc Dao, Van-Dan Nguyen, Dieu-Thuong Thi Trinh, Hieu Kim Huynh, Chi-Bao Bui
Due to the high-cost and limitations of current wound healing treatments, the search for alternative approaches or drugs, particularly from medicinal plants, is of key importance. In this study, we report anti-inflammatory and wound healing activities of the major calophyllolide (CP) compound isolated from Calophyllum inophyllum Linn. The results showed that CP had no effect on HaCaT cell viability over a range of concentrations. CP reduced fibrosis formation and effectively promoted wound closure in mouse model without causing body weight loss...
2017: PloS One
https://www.readbyqxmd.com/read/29018915/-non-cryoconserving-storage-strategies-for-fresh-osteochondral-allografts
#9
REVIEW
Luisa Marilena Schäck, Jan-Dierk Clausen, Sandra Noack, Afif Harb, Christian Krettek, Claudia Neunaber
BACKGROUND: The clinical outcome of fresh allogeneic osteochondral allografts (OCA) is greatly dependent on the number of viable chondrocytes at the time of implantation. The selection and preparation of a suitable recipient can be very time-consuming and the number of tissue donors is greatly limited; therefore, the preservation of high allograft viability before transplantation is a focal point of current research. OBJECTIVE: The objective of this review is to give an overview of established storage strategies for OCA and to serve as a decision-making aid for German clinics in the choice of a suitable storage strategy...
October 10, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/29018432/maize-inoculation-with-azospirillum-brasilense-ab-v5-cells-enriched-with-exopolysaccharides-and-polyhydroxybutyrate-results-in-high-productivity-under-low-n-fertilizer-input
#10
André L M Oliveira, Odair J A P Santos, Paulo R F Marcelino, Karina M L Milani, Mónica Y A Zuluaga, Claudemir Zucareli, Leandro S A Gonçalves
Although Azospirillum strains used in commercial inoculant formulations presents diazotrophic activity, it has been reported that their ability to produce phytohormones plays a pivotal role in plant growth-promotion, leading to a general recommendation of its use in association with regular N-fertilizer doses. In addition, a high variability in the effectiveness of Azospirillum inoculants is still reported under field conditions, contributing to the adoption of the inoculation technology as an additional management practice rather than its use as an alternative practice to the use of chemical inputs in agriculture...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29017900/neural-electrode-resilience-against-dielectric-damage-may-be-improved-by-use-of-highly-doped-silicon-as-a-conductive-material
#11
Ryan Caldwell, Rohit Sharma, Pavel Takmakov, Matthew G Street, Florian Solzbacher, Prashant Tathireddy, Loren Rieth
BACKGROUND: Dielectric damage occurring in vivo to neural electrodes, leading to conductive material exposure and impedance reduction over time, limits the functional lifetime and clinical viability of neuroprosthetics. We used silicon micromachined Utah Electrode Arrays (UEAs) with iridium oxide (IrOx) tip metallization and parylene C dielectric encapsulation to understand the factors affecting device resilience and drive improvements. NEW METHOD: In vitro impedance measurements and finite element analyses were conducted to evaluate how exposed surface area of silicon and IrOx affect UEA properties...
October 7, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29016959/low-boron-tolerance-strategies-involving-the-pectin-mediated-cell-wall-mechanical-properties-in-brassica-napus
#12
Ting Zhou, Yingpeng Hua, Baocai Zhang, Xiuqing Zhang, Yihua Zhou, Lei Shi, Fangsen Xu
Boron (B) is an essential micronutrient for the growth and development of plants. Oilseed rape (Brassica napus L.) is a staple oleaginous crop, which is greatly susceptible to B deficiency. Significant differences in the tolerance to low-B stresses are observed in rapeseed genotypes, whereas the underlying mechanism remains unclear, particularly at the single-cell level. Here, we provide novel insights into the pectin-mediated cell wall (CW) mechanical properties implicated in the differential tolerance to low-B in rapeseed genotypes...
September 15, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29016838/the-hand1-frameshift-a126fs-mutation-does-not-cause-hypoplastic-left-heart-syndrome-in-mice
#13
Beth A Firulli, Kevin P Toolan, Jade Harkin, Hannah Millar, Santiago Pineda, Anthony B Firulli
Aims: To test if a human Hand1 frame shift mutation identified in human samples is causative of hypoplastic left heart syndrome (HLHS). Methods and results: HLHS is a poorly understood single ventricle congenital heart defect that affects two to three infants in every 10 000 live births. The aetiologies of HLHS are largely unknown. The basic helix-loop-helix transcription factor HAND1 is required for normal heart development. Interrogation of HAND1 sequence from fixed HLHS tissues identified a somatic frame-shift mutation at Alanine 126 (NP_004812...
August 31, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/29016820/selective-bcl-xl-inhibition-promotes-apoptosis-in-combination-with-mln8237-in-medulloblastoma-and-pediatric-glioblastoma-cells
#14
Jane Levesley, Lynette Steele, Anke Brüning-Richardson, Adam Davison, Jia Zhou, Chunyong Ding, Sean Lawler, Susan C Short
Background: CNS tumors, including medulloblastoma and pediatric glioblastoma (pGBM) account for the majority of solid pediatric malignancies. There remains an unmet need to identify novel treatment approaches in poor prognosis and relapsed pediatric brain tumors, where therapeutic options are limited. Small-molecule B-cell lymphoma 2 (BCL-2) family inhibitors may enhance tumor cell killing when combined with conventional and targeted chemotherapeutic agents. We investigated the effect of disrupting BCL-2 and B cell lymphoma-extra large (BCL-XL) protein function using ABT-263, ABT-199 and WEHI-539 in medulloblastoma and pGBM cells following treatment with MLN8237, an Aurora kinase inhibitor under investigation as a novel agent for the treatment of malignant brain tumors...
July 20, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28994788/evaluating-primary-blast-effects-in-vitro
#15
Niall J Logan, Hari Arora, Claire A Higgins
Exposure to blast events can cause severe trauma to vital organs such as the lungs, ears, and brain. Understanding the mechanisms behind such blast-induced injuries is of great importance considering the recent trend towards the use of explosives in modern warfare and terrorist-related incidents. To fully understand blast-induced injury, we must first be able to replicate such blast events in a controlled environment using a reproducible method. In this technique using shock tube equipment, shock waves at a range of pressures can be propagated over live cells grown in 2D, and markers of cell viability can be immediately analyzed using a redox indicator assay and the fluorescent imaging of live and dead cells...
September 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28993697/n-terminal-gelsolin-fragment-potentiates-trail-mediated-death-in-resistant-hepatoma-cells
#16
Keith Meyer, Young-Chan Kwon, Ratna B Ray, Ranjit Ray
TNF-α related apoptosis-inducing ligand (TRAIL) selectively kills tumor cells, without damaging normal cells. TRAIL receptors facilitate induction of apoptosis for selective elimination of malignant cells. However, some cancer cells have developed resistances to TRAIL which limits anticancer potential. Gelsolin, a multifunctional actin-binding protein, mediates cell death involving the TRAIL receptors in the hepatic stellate cell line, LX2. Here, we have shown that conditioned medium (CM) containing gelsolin fragments or an N-terminal gelsolin fragment (amino acid residues 1-70) in the presence of TRAIL impairs cell viability of TRAIL resistant transformed human hepatocytes (HepG2)...
October 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28993205/a-novel-technology-to-model-pressure-induced-cellular-injuries-in-the-brain
#17
Michael E Smith, Ramin Eskandari
BACKGROUND: Elevated intracranial pressure (ICP) accompanying a number of neurological emergencies is poorly understood, and lacks a model to determine cellular pathophysiology. This limits our ability to identify cellular and molecular biomarkers associated with the pathological progression from physiologic to pathologic ICP. NEW METHOD: We developed an ex vivo model of pressure-induced brain injury, which combines 3D neural cell cultures and a newly developed Pressure Controlled Cell Culture Incubator (PC(3)I)...
October 6, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28993129/separating-the-agony-from-ecstasy-r-3-4-methylenedioxymethamphetamine-has-prosocial-and-therapeutic-like-effects-without-signs-of-neurotoxicity-in-mice
#18
Daniel W Curry, Matthew B Young, Andrew N Tran, Georges E Daoud, Leonard L Howell
S,R(+/-)-3,4-methylenedioxymethamphetamine (SR-MDMA) is an amphetamine derivative with prosocial and putative therapeutic effects. Ongoing clinical trials are investigating it as a treatment for post-traumatic stress disorder (PTSD) and other conditions. However, its potential for adverse effects such as hyperthermia and neurotoxicity may limit its clinical viability. We investigated the hypothesis that one of the two enantiomers of SR-MDMA, R-MDMA, would retain the prosocial and therapeutic effects but with fewer adverse effects...
October 6, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28991381/rational-in-silico-design-of-an-organic-semiconductor-with-improved-electron-mobility
#19
Pascal Friederich, Verónica Gómez, Christian Sprau, Velimir Meded, Timo Strunk, Michael Jenne, Andrea Magri, Franz Symalla, Alexander Colsmann, Mario Ruben, Wolfgang Wenzel
Organic semiconductors find a wide range of applications, such as in organic light emitting diodes, organic solar cells, and organic field effect transistors. One of their most striking disadvantages in comparison to crystalline inorganic semiconductors is their low charge-carrier mobility, which manifests itself in major device constraints such as limited photoactive layer thicknesses. Trial-and-error attempts to increase charge-carrier mobility are impeded by the complex interplay of the molecular and electronic structure of the material with its morphology...
October 9, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28990205/developing-a-protocol-for-normothermic-ex-situ-limb-perfusion
#20
Eliana F R Duraes, Maria Madajka, Russell Frautschi, Basem Soliman, Cagri Cakmakoglu, Addison Barnett, Kashyap Tadisina, Qiang Liu, Patrick Grady, Cristiano Quintini, Toshihiro Okamoto, Francis Papay, Antonio Rampazzo, Bahar Bassiri Gharb
BACKGROUND: Ischemia time represents a significant limitation for successful extremity transplantation because of the rapid deterioration of ischemic muscle. Normothermic ex-situ preservation is an emergent method to prolong the organ viability following procurement, by replicating the physiologic conditions. The aim of this study was to develop an ex-situ normothermic limb perfusion system to preserve the viability and function of porcine limbs for 12 hours following procurement. METHODS: A total of 18 swine limbs were perfused...
October 9, 2017: Microsurgery
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