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https://www.readbyqxmd.com/read/28645304/validation-of-an-automated-technique-for-ovarian-cortex-dissociation-isolation-of-viable-ovarian-cells-and-their-qualification-by-multicolor-flow-cytometry
#1
Tristan Zver, Elodie Mouloungui, Aurélie Berdin, Christophe Roux, Clotilde Amiot
BACKGROUND: Ovarian tissue cryopreservation is a technique for fertility preservation addressed to prepubertal girls or to patients for whom no ovarian stimulation is possible before initiation of gonadotoxic treatments. Autotransplantation of frozen-thawed ovarian tissue is the only available option for reuse but presents some limitations: ischemic tissue damages post-transplant and reintroduction of malignant cells in cases of cancer. It is therefore essential to qualify ovarian tissue before autograft on a functional and oncological point of view...
June 23, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28644033/direct-indirect-nature-of-the-bandgap-in-lead-free-perovskite-nanocrystals
#2
Yuhai Zhang, Jun Yin, Manas R Parida, Ghada H Ahmed, Jun Pan, Osman M Bakr, Jean-Luc Bredas, Omar F Mohammed
With record efficiencies achieved in lead halide perovskite-based photovoltaics, urgency has shifted towards finding alternative materials that are stable and less-toxic. Bismuth-based perovskite materials are currently one of the most promising candidates amongst those alternatives. However, the band structures of these materials, including the nature of the bandgaps, remain elusive due to extremely low photoluminescence quantum yield (PLQY) and scattering issues in their thin-film or bulk form. Here, we overcome those limitations and reveal the specific nature of the material's electronic transitions by realizing monodisperse colloidal nanocrystals (NCs) of hexagonal-phase Cs3Bi2X9 perovskites, which afford well-resolved PL features...
June 23, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28638116/laser-ultrasonic-delivery-of-agents-into-articular-cartilage
#3
Heikki J Nieminen, Gonçalo Barreto, Mikko A Finnilä, Alejandro García-Pérez, Ari Salmi, Sanjeev Ranjan, Kari K Eklund, Kenneth P H Pritzker, Simo Saarakkala, Edward Hæggström
Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound (laser-induced plasma sound source) to deliver agents (methylene blue, MB, in PBS) into bovine AC. Treatment samples (n = 10) were immersed in MB solution simultaneously with LU exposure, while adjacent control 1 tissue (n = 10) was pre-treated with LU followed by immersion in MB and adjacent control 2 tissue (n = 10) was only immersed in MB...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637493/the-role-of-nlrp3-inflammasome-in-5-fluorouracil-resistance-of-oral-squamous-cell-carcinoma
#4
Xiaodong Feng, Qingqiong Luo, Han Zhang, Han Wang, Wantao Chen, Guangxun Meng, Fuxiang Chen
BACKGROUND: 5-Fluorouracil (5-FU) is a widely used drug for the therapy of cancer. However, the chemoresistance of tumor cells to 5-FU usually limits its clinical effectiveness. In this study, we explored the role of NLRP3 inflammasome in 5-FU resistance of oral squamous cell carcinoma (OSCC). METHODS: The mRNA and protein expression levels of NLRP3, Caspase1 and IL-1β in resected OSCC specimens or cell lines were measured respectively by quantitative real time-PCR (qRT-PCR) and western blot...
June 21, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28637492/expression-patterns-of-fshd-causing-dux4-and-myogenic-transcription-factors-pax3-and-pax7-are-spatially-distinct-in-differentiating-human-stem-cell-cultures
#5
Premi Haynes, Kelly Kernan, Suk-Lin Zhou, Daniel G Miller
BACKGROUND: Facioscapulohumeral muscular dystrophy (FSHD) is most commonly inherited in an autosomal dominant pattern and caused by the abnormal expression of DUX4 in skeletal muscle. The DUX4 transcription factor has DNA binding domains similar to several paired class homeotic transcription factors, but only myogenic factors PAX3 and PAX7 rescue cell viability when co-expressed with DUX4 in mouse myoblasts. This observation suggests competition for DNA binding sites in satellite cells might limit muscle repair and may be one aspect of DUX4-associated myotoxicity...
June 21, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/28637488/a-high-throughput-colorimetric-assay-for-detection-of-schistosoma-mansoni-viability-based-on-the-tetrazolium-salt-xtt
#6
Pedro Henrique Nascimento Aguiar, Núbia Monteiro Gonçalves Soares Fernandes, Carlos Leomar Zani, Marina Moraes Mourão
BACKGROUND: Schistosoma mansoni is a trematode parasite that causes schistosomiasis, one of the most prevalent neglected tropical diseases, leading to the loss of 2.6 million disability-adjusted life years. Praziquantel is the only drug available, and new drugs are required. The most common strategy in schistosomiasis drug discovery is the use of the schistosomula larval-stage for a pre-screen in drug sensitivity assays. However, assessing schistosomula viability by microscopy has always been a limitation to the throughput of such assays...
June 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28634289/sex-specific-early-survival-drives-adult-sex-ratio-bias-in-snowy-plovers-and-impacts-mating-system-and-population-growth
#7
Luke J Eberhart-Phillips, Clemens Küpper, Tom E X Miller, Medardo Cruz-López, Kathryn H Maher, Natalie Dos Remedios, Martin A Stoffel, Joseph I Hoffman, Oliver Krüger, Tamás Székely
Adult sex ratio (ASR) is a central concept in population biology and a key factor in sexual selection, but why do most demographic models ignore sex biases? Vital rates often vary between the sexes and across life history, but their relative contributions to ASR variation remain poorly understood-an essential step to evaluate sex ratio theories in the wild and inform conservation. Here, we combine structured two-sex population models with individual-based mark-recapture data from an intensively monitored polygamous population of snowy plovers...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28633471/development-of-a-chicken-ileal-explant-culture-model-for-measurement-of-gut-inflammation-induced-by-lipopolysaccharide
#8
Qian Zhang, Susan D Eicher, Kolapo M Ajuwon, Todd J Applegate
Gut mucosa holds a single layer of epithelial cells and the largest mass of lymphoid tissue in the body. Although the epithelial cell culture model is widely used to assess intestinal barrier function, it has limitations for studying cellular interactions, in particular those of the immune system. In this study, a chicken ileal explant culture model was developed for investigating short-term gut inflammatory and secretory responses in an ex vivo environment. Initially, ileal explants from broilers at 21 d of age were cultured ex vivo up to 6 h...
June 16, 2017: Poultry Science
https://www.readbyqxmd.com/read/28632379/intracellular-delivery-platform-for-recalcitrant-cells-when-polymeric-carrier-marries-photoporation
#9
Jingxian Wu, Hui Xue, Zhonglin Lyu, Zhenhua Li, Yangcui Qu, Yajun Xu, Lei Wang, Qian Yu, Hong Chen
The intracellular delivery of exogenous macromolecules is of great interest for both fundamental biological research and clinical applications. Although traditional delivery systems based on either carrier mediation or membrane disruption have some advantages; however, they are generally limited with respect to delivery efficiency and cytotoxicity. Herein, a collaborative intracellular delivery platform with excellent comprehensive performance is developed using polyethylenimine of low molecular weight (LPEI) as a gene carrier in conjunction with a gold nanoparticle layer (GNPL) acting as a photoporation agent...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28631080/comparison-of-volumetric-and-functional-parameters-in-simultaneous-cardiac-pet-mr-feasibility-of-volumetric-assessment-with-residual-activity-from-prior-pet-ct
#10
C Lücke, B Oppolzer, P Werner, B Foldyna, P Lurz, T Jochimsen, B Brenneis, L Lehmkuhl, B Sattler, M Grothoff, H Barthel, O Sabri, M Gutberlet
OBJECTIVE: To compare cardiac left ventricular (LV) parameters in simultaneously acquired hybrid fluorine-18-fluorodeoxyglucose ([18F] FDG) positron emission tomography/magnetic resonance imaging (PET/MRI) in patients with residual tracer activity of upstream PET/CT. METHODS: Twenty-nine patients (23 men, age 58±17 years) underwent cardiac PET/MRI either directly after a non-cardiac PET/CT with homogenous cardiac [18F] FDG uptake (n=20) or for viability assessment (n=9)...
June 19, 2017: European Radiology
https://www.readbyqxmd.com/read/28630930/real-time-quantitative-analysis-of-metabolic-flux-in-live-cells-using-a-hyperpolarized-micromagnetic-resonance-spectrometer
#11
Sangmoo Jeong, Roozbeh Eskandari, Sun Mi Park, Julio Alvarez, Sui Seng Tee, Ralph Weissleder, Michael G Kharas, Hakho Lee, Kayvan R Keshari
Metabolic reprogramming is widely considered a hallmark of cancer, and understanding metabolic dynamics described by the conversion rates or "fluxes" of metabolites can shed light onto biological processes of tumorigenesis and response to therapy. For real-time analysis of metabolic flux in intact cells or organisms, magnetic resonance (MR) spectroscopy and imaging methods have been developed in conjunction with hyperpolarization of nuclear spins. These approaches enable noninvasive monitoring of tumor progression and treatment efficacy and are being tested in multiple clinical trials...
June 2017: Science Advances
https://www.readbyqxmd.com/read/28630928/novel-phase-diagram-behavior-and-materials-design-in-heterostructural-semiconductor-alloys
#12
Aaron M Holder, Sebastian Siol, Paul F Ndione, Haowei Peng, Ann M Deml, Bethany E Matthews, Laura T Schelhas, Michael F Toney, Roy G Gordon, William Tumas, John D Perkins, David S Ginley, Brian P Gorman, Janet Tate, Andriy Zakutayev, Stephan Lany
Structure and composition control the behavior of materials. Isostructural alloying is historically an extremely successful approach for tuning materials properties, but it is often limited by binodal and spinodal decomposition, which correspond to the thermodynamic solubility limit and the stability against composition fluctuations, respectively. We show that heterostructural alloys can exhibit a markedly increased range of metastable alloy compositions between the binodal and spinodal lines, thereby opening up a vast phase space for novel homogeneous single-phase alloys...
June 2017: Science Advances
https://www.readbyqxmd.com/read/28630443/chemical-chaperone-tudca-unlike-pba-mitigates-protein-aggregation-efficiently-and-resists-er-and-non-er-stress-induced-hepg2-cell-death
#13
Jagadeesh Kumar Uppala, Amina R Gani, Kolluru V A Ramaiah
Stress induced BSA (bovine serum albumin) protein aggregation is effectively mitigated in vitro by TUDCA (tauroursodeoxycholic acid) than by PBA (4- phenylbutyric acid), chemical chaperones approved by FDA for the treatment of biliary cirrhosis and urea cycle disorders respectively. TUDCA, unlike PBA, enhances trypsin mediated digestion of BSA. TUDCA activates PERK, an ER-resident kinase that phosphorylates the alpha-subunit of eukaryotic initiation factor2 (eIF2α) and promotes the expression of activated transcription factor 4 (ATF4) in HepG2 cells...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28627179/water-lean-solvents-for-post-combustion-co2-capture-fundamentals-uncertainties-opportunities-and-outlook
#14
David J Heldebrant, Phillip K Koech, Vassiliki-Alexandra Glezakou, Roger Rousseau, Deepika Malhotra, David C Cantu
This review is designed to foster the discussion regarding the viability of postcombustion CO2 capture by water-lean solvents, by separating fact from fiction for both skeptics and advocates. We highlight the unique physical and thermodynamic properties of notable water-lean solvents, with a discussion of how such properties could translate to efficiency gains compared to aqueous amines. The scope of this review ranges from the purely fundamental molecular-level processes that govern solvent behavior to bench-scale testing, through process engineering and projections of process performance and cost...
June 19, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28626431/artificial-intelligence-assisted-online-social-therapy-for-youth-mental-health
#15
Simon D'Alfonso, Olga Santesteban-Echarri, Simon Rice, Greg Wadley, Reeva Lederman, Christopher Miles, John Gleeson, Mario Alvarez-Jimenez
Introduction: Benefits from mental health early interventions may not be sustained over time, and longer-term intervention programs may be required to maintain early clinical gains. However, due to the high intensity of face-to-face early intervention treatments, this may not be feasible. Adjunctive internet-based interventions specifically designed for youth may provide a cost-effective and engaging alternative to prevent loss of intervention benefits. However, until now online interventions have relied on human moderators to deliver therapeutic content...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28625364/semi-quantitative-models-for-identifying-potent-and-selective-transthyretin-amyloidogenesis-inhibitors
#16
Stephen Connelly, David E Mortenson, Sungwook Choi, Ian A Wilson, Evan T Powers, Jeffery W Kelly, Steven M Johnson
Rate-limiting dissociation of the tetrameric protein transthyretin (TTR), followed by monomer misfolding and misassembly, appears to cause degenerative diseases in humans known as the transthyretin amyloidoses, based on human genetic, biochemical and pharmacologic evidence. Small molecules that bind to the generally unoccupied thyroxine binding pockets in the native TTR tetramer kinetically stabilize the tetramer, slowing subunit dissociation proportional to the extent that the molecules stabilize the native state over the dissociative transition state-thereby inhibiting amyloidogenesis...
May 26, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28625015/crispr-cas9-mediated-lag-3-disruption-in-car-t-cells
#17
Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang
T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The immunosuppressive nature of tumor microenvironment is considered one of the key factors limiting CAR-T efficacy. One negative regulator of Tcell activity is lymphocyte activation gene-3 (LAG-3). We successfully generated LAG-3 knockout Tand CAR-T cells with high efficiency using CRISPR-Cas9 mediated gene editing and found that the viability and immune phenotype were not dramatically changed during in vitro culture...
June 17, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28623712/deoxypodophyllotoxin-induces-cytoprotective-autophagy-against-apoptosis-via-inhibition-of-pi3k-akt-mtor-pathway-in-osteosarcoma-u2os-cells
#18
Sang-Hun Kim, Kyo-Min Son, Kwang-Youn Kim, Sun-Nyoung Yu, Sul-Gi Park, Young-Wook Kim, Hyo-Won Nam, Jeung-Tak Suh, Jae-Hoon Ji, Soon-Cheol Ahn
BACKGROUND: A natural compound deoxypodophyllotoxin (DPT) possesses potent anti-proliferative and anti-tumor properties on several cancer types. It triggers cell cycle arrest followed by apoptosis through various cellular processes. However, it is limited to the action mechanism of DPT-mediated cell death modes via apoptosis and autophagy. METHODS: Cell viability assay, morphological changes, annexin-V/propidium iodide (PI) assay, reactive oxygen species (ROS), acridine orange staining, and Western blot analyses were evaluated...
April 13, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28623695/injectable-cytocompatible-elastic-free-radical-scavenging-and-electroconductive-hydrogel-for-cardiac-cell-encapsulation
#19
Remya Komeri, Jayabalan Muthu
The injectable electroconductive hydrogels are desirable for the regenerative therapy of electroresponsive tissues like heart. With the present electroconductive hydrogels, the issues of cytotoxicity, biodegradability, and diffusion of the conductive element and poor water solubility limit their applications. Here, electroconductive injectable single component hydrogels, PANIE-P/PEGDA and PANIS-P/PEGDA, are prepared with fumarate-co-PEG-co-sebacate comacromer conjugated with non-sulfonated/sulfonated polyaniline and PEGDA...
June 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28622897/the-efficacy-of-local-autologous-bone-dust-a-systematic-review
#20
Matthew Street, Ryan Gao, Waldron Martis, Jacob Munro, David Musson, Jillian Cornish, John Ferguson
STUDY DESIGN: A systematic review of bone dust as an autologous bone graft to encourage osseous fusion. OBJECTIVE: To identify and review studies that report on the therapeutic potential of bone dust. The research question was structured as follows: populations-animal and human sources of bone dust harvested using burrs; interventions-autologous bone dust compared with other clinically utilized bone graft options; outcomes assessed-(1) in vitro cell viability, cell differentiation, and osteogenic potential and (2) clinical efficacy in the form of fusion rates as assessed using plain radiographs; study designs-in vitro, preclinical in vivo and clinical studies investigating the therapeutic potential of bone dust, harvested by burring, are included in this systematic review...
July 2017: Spine Deformity
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