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Fatih Saban Beris, Lina De Smet, Hakan Karaoglu, Sabriye Canakci, Jozef Van Beeumen, Ali Osman Belduz
The G2ALT gene was cloned and sequenced from the thermophilic bacterium Anoxybacillus gonensis G2. The gene is 666 bp long and encodes a protein 221 amino acids in length. The gene was overexpressed in E. coli and purified to homogeneity and biochemically characterized. The enzyme has a molecular mass of 24.5 kDa and it could be classified as a member of the family of bacterial aluminium resistance proteins based on homology searches. When this fragment was expressed in E. coli, it endowed E. coli with Al tolerance to 500 μM...
August 2011: Journal of Microbiology / the Microbiological Society of Korea
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