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https://www.readbyqxmd.com/read/28835937/leukemia-inhibitory-factor-increases-the-proliferation-of-human-endometrial-stromal-cells-and-expression-of-genes-related-to-pluripotency
#1
Mojdeh Salehnia, Mehri Fayazi, Shokreya Ehsani
BACKGROUND: Concerning the low population of human endometrial mesenchymal cells within the tissue and their potential application in the clinic and tissue engineering, some researches have been focused on their in vitro expansion. OBJECTIVE: The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) as a proliferative factor on the expansion and proliferation of human endometrial stromal cells. MATERIALS AND METHODS: In this experimental study, the isolated and cultured human endometrial stromal cells from women at ovulatory phase aged 20-35 years, after fourth passage were divided into control and LIF-treated groups...
April 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28751909/simultaneous-methylation-level-assessment-of-hundreds-of-cpg-sites-by-targeted-bisulfite-pcr-sequencing-tbpseq
#2
Kyuheum Jeon, Byungkuk Min, Jung S Park, Yong-Kook Kang
Methylated-DNA sequencing technologies are producing vast amounts of methylome data from cancer samples, from which cancer-associated differentially methylated CpG sites (cDMCs) are continuously identified and filed. The inclusion of as many cDMCs as possible helps improve the accuracy of cancer diagnosis and sometimes identify cancer subtypes. However, the lack of an established method for the analysis of 100s of cDMCs practically impedes their robust use in clinical medicine. Here, we tested the availability of targeted bisulfite-PCR-sequencing (TBPseq) technology for the assessment of methylation levels of a myriad of CpGs scattered over the genome...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28728562/low-oxygen-tension-increases-mitochondrial-membrane-potential-and-enhances-expression-of-antioxidant-genes-and-implantation-protein-of-mouse-blastocyst-cultured-in-vitro
#3
Yun-Yi Ma, Huei-Wen Chen, Chii-Ruey Tzeng
BACKGROUND: In human IVF, embryos cultured with a lower O2 tension (5%) can give rise to higher success rates when compared with normoxic conditions (20%). However, the mechanisms behind the beneficial effects of reduced oxygen tension in embryogenesis remain unclear. The aim of this study was to evaluate the expression of oxygen related and antioxidant genes and mitochondrial function in mouse embryo cultured under hypoxic and normoxic conditions, to investigate the beneficial effect of low oxygen tension in preimplantation embryogenesis...
July 20, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28629464/mir-629-3p-may-serve-as-a-novel-biomarker-and-potential-therapeutic-target-for-lung-metastases-of-triple-negative-breast-cancer
#4
Jin Wang, Cailu Song, Hailin Tang, Chao Zhang, Jun Tang, Xing Li, Bo Chen, Xiaoming Xie
BACKGROUND: Different breast cancer subtypes show distinct tropisms for sites of metastasis. Notably, the lung is the most common site for the first distant recurrence in triple-negative breast cancer (TNBC). The identification of novel biomarkers for lung metastasis is of great importance to improving the outcome of TNBC. In this study, we sought to identify a microRNA (miRNA)-based biomarker and therapeutic target for lung metastasis of TNBC. METHODS: A total of 669 patients without de novo stage IV TNBC were recruited for this study...
June 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28533357/tlr9-expression-and-secretion-of-lif-by-prostate-cancer-cells-stimulates-accumulation-and-activity-of-polymorphonuclear-mdscs
#5
Haejung Won, Dayson Moreira, Chan Gao, Priyanka Duttagupta, Xingli Zhao, Edwin Manuel, Don Diamond, Yate-Ching Yuan, Zheng Liu, Jeremy Jones, Massimo D'Apuzzo, Sumanta Pal, Marcin Kortylewski
Proinflammatory signals promote prostate tumorigenesis and progression, but their origins and downstream effects remain unclear. We recently demonstrated that the expression of an innate immune receptor, TLR9, by prostate cancer cells is critical for their tumor-propagating potential. We investigated whether cancer cell-intrinsic TLR9 signaling alters composition of the prostate tumor microenvironment. We generated Ras/Myc (RM9) and Myc-driven (Myc-CaP) prostate cancer cells expressing the tetracycline-inducible gene Tlr9 (Tlr9(ON) ) or the control LacZ (LacZ(ON) )...
August 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28433851/dna-methylation-regulates-gene-expression-in-intracranial-aneurysms
#6
Lanbing Yu, Jia Wang, Shuo Wang, Dong Zhang, Yuanli Zhao, Rong Wang, Jizong Zhao
BACKGROUND: Different gene expression profiles are observed in intracranial aneurysm tissues. Understanding these genes and what regulates their expression will provide insight into the pathogenesis of intracranial aneurysms. We investigated whether differences in DNA methylation regulate gene expression in intracranial aneurysms. METHODS: We compared 20 intracranial aneurysm tissue specimens with 20 matched specimens from the superficial temporal artery as controls...
September 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28411194/systematic-characterization-of-a-to-i-rna-editing-hotspots-in-micrornas-across-human-cancers
#7
Yumeng Wang, Xiaoyan Xu, Shuangxing Yu, Kang Jin Jeong, Zhicheng Zhou, Leng Han, Yiu Huen Tsang, Jun Li, Hu Chen, Lingegowda S Mangala, Yuan Yuan, A Karina Eterovic, Yiling Lu, Anil K Sood, Kenneth L Scott, Gordon B Mills, Han Liang
RNA editing, a widespread post-transcriptional mechanism, has emerged as a new player in cancer biology. Recent studies have reported key roles for individual miRNA editing events, but a comprehensive picture of miRNA editing in human cancers remains largely unexplored. Here, we systematically characterized the miRNA editing profiles of 8595 samples across 20 cancer types from miRNA sequencing data of The Cancer Genome Atlas and identified 19 adenosine-to-inosine (A-to-I) RNA editing hotspots. We independently validated 15 of them by perturbation experiments in several cancer cell lines...
July 2017: Genome Research
https://www.readbyqxmd.com/read/28371410/new-genes-associated-with-rheumatoid-arthritis-identified-by-gene-expression-profiling
#8
H Wang, J Guo, J Jiang, W Wu, X Chang, H Zhou, Z Li, J Zhao
In this study, we aimed to find new genes associated with rheumatoid arthritis (RA) so that more comprehensive genes would be used for monitoring and/or diagnosing patients. Illumina digital gene expression profiling was applied in two sample types - peripheral blood mononuclear cells (PBMCs) and synovial cells to compare the gene expression pattern between 17 patients with RA and three control groups (six osteoarthritis patients, three ankylosing spondylitis patients and 17 healthy controls). Bioinformatics was performed on pathway analysis and protein-protein interaction networks...
April 2, 2017: International Journal of Immunogenetics
https://www.readbyqxmd.com/read/28368537/tissue-specific-ablation-of-the-lif-receptor-in-the-murine-uterine-epithelium-results-in-implantation-failure
#9
JrGang Cheng, Gracy Rosario, Tatiana V Cohen, Jianbo Hu, Colin L Stewart
The cytokine leukemia inhibitory factor (LIF) is essential for rendering the uterus receptive for blastocyst implantation. In mice, LIF receptor expression (LIFR) is largely restricted to the uterine luminal epithelium (LE). LIF, secreted from the endometrial glands (GEs), binds to the LIFR, activating the Janus kinase-signal transducer and activation of transcription (STAT) 3 (Jak-Stat3) signaling pathway in the LE. JAK-STAT activation converts the LE to a receptive state so that juxtaposed blastocysts begin to implant...
June 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28334964/mutations-in-the-leukemia-inhibitory-factor-receptor-lifr-gene-and-lifr-deficiency-cause-urinary-tract-malformations
#10
Anne Kosfeld, Frank Brand, Anna-Carina Weiss, Martin Kreuzer, Michaela Goerk, Helge Martens, Stephanie Schubert, Anne-Kathrin Schäfer, Vera Riehmer, Imke Hennies, Jan Hinrich Bräsen, Lars Pape, Kerstin Amann, Lars Krogvold, Anna Bjerre, Christoph Daniel, Andreas Kispert, Dieter Haffner, Ruthild G Weber
Congenital anomalies of the kidneys and urinary tract (CAKUT) are the most common cause of chronic kidney disease in children. As CAKUT is a genetically heterogeneous disorder and most cases are genetically unexplained, we aimed to identify new CAKUT causing genes. Using whole-exome sequencing and trio-based de novo analysis, we identified a novel heterozygous de novo frameshift variant in the leukemia inhibitory factor receptor (LIFR) gene causing instability of the mRNA in a patient presenting with bilateral CAKUT and requiring kidney transplantation at one year of age...
May 1, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28244758/glycosylation-changes-in-serum-proteins-identify-patients-with-pancreatic-cancer
#11
Anna Drabik, Anna Bodzon-Kulakowska, Piotr Suder, Jerzy Silberring, Jan Kulig, Marek Sierzega
After more than a decade of biomarker discovery using advanced proteomic and genomic approaches, very few biomarkers have been involved in clinical diagnostics. Most candidate biomarkers are focused on the protein component. Targeting post-translational modifications (PTMs) in combination with protein sequences will provide superior diagnostic information with regards to sensitivity and specificity. Glycosylation is one of the most common and functionally important PTMs. It plays a central role in many biological processes, including protein folding, host-pathogen interactions, immune response, and inflammation...
March 20, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28228280/autocrine-loop-involving-il-6-family-member-lif-lif-receptor-and-stat4-drives-sustained-fibroblast-production-of-inflammatory-mediators
#12
Hung N Nguyen, Erika H Noss, Fumitaka Mizoguchi, Christine Huppertz, Kevin S Wei, Gerald F M Watts, Michael B Brenner
Fibroblasts are major contributors to and regulators of inflammation and dominant producers of interleukin-6 (IL-6) in inflammatory diseases like rheumatoid arthritis. Yet, compared to leukocytes, the regulation of inflammatory pathways in fibroblasts is largely unknown. Here, we report that analyses of genes coordinately upregulated with IL-6 pointed to STAT4 and leukemia inhibitory factor (LIF) as potentially linked. Gene silencing revealed that STAT4 was required for IL-6 transcription. STAT4 was recruited to the IL-6 promoter after fibroblast activation, and LIF receptor (LIFR) and STAT4 formed a molecular complex that, together with JAK1 and TYK2 kinases, controlled STAT4 activation...
February 21, 2017: Immunity
https://www.readbyqxmd.com/read/28122243/opposing-roles-of-acetylation-and-phosphorylation-in-lifr-dependent-self-renewal-growth-signaling-in-mouse-embryonic-stem-cells
#13
Xiong-Jun Wang, Yunbo Qiao, Minzhe M Xiao, Lingbo Wang, Jun Chen, Wenjian Lv, Li Xu, Yan Li, Yumei Wang, Ming-Dian Tan, Chao Huang, Jinsong Li, Ting C Zhao, Zhaoyuan Hou, Naihe Jing, Y Eugene Chin
LIF promotes self-renewal of mouse embryonic stem cells (mESCs), and in its absence, the cells differentiate. LIF binds to the LIF receptor (LIFR) and activates the JAK-STAT3 pathway, but it remains unknown how the receptor complex triggers differentiation or self-renewal. Here, we report that the LIFR cytoplasmic domain contains a self-renewal domain within the juxtamembrane region and a differentiation domain within the C-terminal region. The differentiation domain contains four SPXX repeats that are phosphorylated by MAPK to restrict STAT3 activation; the self-renewal domain is characterized by a 3K motif that is acetylated by p300...
January 24, 2017: Cell Reports
https://www.readbyqxmd.com/read/28116096/an-inhaled-dose-of-budesonide-induces-genes-involved-in-transcription-and-signaling-in-the-human-airways-enhancement-of-anti-and-proinflammatory-effector-genes
#14
Richard Leigh, Mahmoud M Mostafa, Elizabeth M King, Christopher F Rider, Suharsh Shah, Curtis Dumonceaux, Suzanne L Traves, Andrew McWhae, Tyler Kolisnik, Cora Kooi, Donna M Slater, Margaret M Kelly, Mark Bieda, Anna Miller-Larsson, Robert Newton
Although inhaled glucocorticoids, or corticosteroids (ICS), are generally effective in asthma, understanding their anti-inflammatory actions in vivo remains incomplete. To characterize glucocorticoid-induced modulation of gene expression in the human airways, we performed a randomized placebo-controlled crossover study in healthy male volunteers. Six hours after placebo or budesonide inhalation, whole blood, bronchial brushings, and endobronchial biopsies were collected. Microarray analysis of biopsy RNA, using stringent (≥2-fold, 5% false discovery rate) or less stringent (≥1...
August 2016: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/27903796/decreased-endometrial-expression-of-leukemia-inhibitory-factor-receptor-disrupts-the-stat3-signaling-in-adenomyosis-during-the-implantation-window
#15
Chih-Feng Yen, Shuen-Kuei Liao, S Joseph Huang, Selcuk Tabak, Felice Arcuri, Chyi-Long Lee, Aydin Arici, Felice Petraglia, Hsin-Shih Wang, Umit A Kayisli
BACKGROUND: Adenomyosis was found to have negative impacts on embryo implantation. Leukemia inhibitory factor (LIF), proposed to be a molecular marker for endometrial receptivity, works through the LIF receptor (LIFR) on both the embryo and the endometrium. We aimed to evaluate the endometrial expression of LIF and LIFR and its subsequent signaling in patients with adenomyosis during the window of implantation (WOI). METHODS: Endometrium was obtained during the WOI from patients with adenomyosis (age <45 years) who underwent hysterectomy and from age-matched controls who had no endometriosis or adenomyosis...
January 1, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27825285/leukemia-inhibitory-factor-contributes-to-reactive-astrogliosis-via-activation-of-signal-transducer-and-activator-of-transcription-3-signaling-after-intracerebral-hemorrhage-in-rats
#16
Hua-Jun Zhou, Xi Yang, Han-Jin Cui, Tao Tang, Jian-Hua Zhong, Jie-Kun Luo, A-Li Yang, Qi-Mei Zhang, Jing-Hua Zhou, Qiang Zhang
Reactive astrogliosis has occurred after intracerebral hemorrhage (ICH). Leukemia inhibitory factor (LIF) can act as a modulator for glial gene expression. Signal transducer and activator of transcription 3 (STAT3) is a critical regulator of reactive astrogliosis. The present study tested whether endogenous LIF acted on ICH-induced reactive astrogliosis via the STAT3 signaling pathway. Rats were divided into three experimental groups: 1) Rats received either an ICH or a needle insertion (sham), 2) Rats received 100 ng LIF or an equal volume of phosphate-buffered saline (PBS) by direct infusion into the lateral ventricle (LV) after ICH, and 3) AG490 (0...
April 15, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/27784904/erratum-induction-of-lifr-confers-a-dormancy-phenotype-in-breast-cancer-cells-disseminated-to-the-bone-marrow
#17
Rachelle W Johnson, Elizabeth C Finger, Monica M Olcina, Marta Vilalta, Todd Aguilera, Yu Miao, Alyssa R Merkel, Joshua R Johnson, Julie A Sterling, Joy Y Wu, Amato J Giaccia
No abstract text is available yet for this article.
October 27, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27642788/induction-of-lifr-confers-a-dormancy-phenotype-in-breast-cancer-cells-disseminated-to-the-bone-marrow
#18
Rachelle W Johnson, Elizabeth C Finger, Monica M Olcina, Marta Vilalta, Todd Aguilera, Yu Miao, Alyssa R Merkel, Joshua R Johnson, Julie A Sterling, Joy Y Wu, Amato J Giaccia
Breast cancer cells frequently home to the bone marrow, where they may enter a dormant state before forming a bone metastasis. Several members of the interleukin-6 (IL-6) cytokine family are implicated in breast cancer bone colonization, but the role for the IL-6 cytokine leukaemia inhibitory factor (LIF) in this process is unknown. We tested the hypothesis that LIF provides a pro-dormancy signal to breast cancer cells in the bone. In breast cancer patients, LIF receptor (LIFR) levels are lower with bone metastases and are significantly and inversely correlated with patient outcome and hypoxia gene activity...
September 19, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27622335/feedback-activation-of-leukemia-inhibitory-factor-receptor-limits-response-to-histone-deacetylase-inhibitors-in-breast-cancer
#19
Hanlin Zeng, Jia Qu, Nan Jin, Jun Xu, Chenchu Lin, Yi Chen, Xinying Yang, Xiang He, Shuai Tang, Xiaojing Lan, Xiaotong Yang, Ziqi Chen, Min Huang, Jian Ding, Meiyu Geng
Histone deacetylase (HDAC) inhibitors have demonstrated clinical benefits in subtypes of hematological malignancies. However, the efficacy of HDAC inhibitors in solid tumors remains uncertain. This study takes breast cancer as a model to understand mechanisms accounting for limited response of HDAC inhibitors in solid tumors and to seek combination solutions. We discover that feedback activation of leukemia inhibitory factor receptor (LIFR) signaling in breast cancer limits the response to HDAC inhibition. Mechanistically, HDAC inhibition increases histone acetylation at the LIFR gene promoter, which recruits bromodomain protein BRD4, upregulates LIFR expression, and activates JAK1-STAT3 signaling...
September 12, 2016: Cancer Cell
https://www.readbyqxmd.com/read/27562703/water-extracted-perilla-frutescens-increases-endometrial-receptivity-though-leukemia-inhibitory-factor-dependent-expression-of-integrins
#20
Eun-Yeong Kim, Hee-Jung Choi, Tae-Wook Chung, Jun-Yong Choi, Hyung Sik Kim, Yeon-Seop Jung, Syng-Ook Lee, Ki-Tae Ha
The leaves and stems of Perilla frutescens var. acuta Kudo (PF) have been used to prevent threatened abortion in traditional medicine in the East Asian countries. Because reduced receptivity of endometrium is a cause of abortion, we analyzed the action of PF on the endometrial receptivity. PF increased the level of leukemia inhibitory factor (LIF), a major cytokine regulating endometrial receptivity, and LIF receptor in human endometrial Ishikawa cells. The PF-induced LIF expression was mediated by c-jun N-terminal kinase (JNK) and p38 pathways...
August 2016: Journal of Pharmacological Sciences
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