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https://www.readbyqxmd.com/read/28736222/hypermucoviscosity-rmpa-and-aerobactin-are-associated-with-community-acquired-klebsiella-pneumoniae-bacteremic-isolates-causing-liver-abscess-in-singapore
#1
Thean Yen Tan, Melissa Ong, Yvonne Cheng, Lily Siew Yong Ng
INTRODUCTION: This retrospective study investigated the clinical etiology of community-acquired bacteremic Klebsiellapneumoniae infections, and characterized laboratory and genetic markers which may be associated with primary liver abscess (PLA). METHODS: Community-onset K. pneumoniae bacteremic episodes from 2010 to 2011 were identified from the laboratory information system. Isolates were retrieved for susceptibility testing, hypermucoviscosity testing, PCR-based serotyping (K1, K2 and K5) and PCR detection of virulence genes (rmpA, alls, kfu and aerobactin)...
July 14, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28712037/introduction-of-sample-tubes-with-sodium-azide-as-a-preservative-for-ethyl-glucuronide-in-urine
#2
Marc Luginbühl, Wolfgang Weinmann, Ali Al-Ahmad
Ethyl glucuronide (EtG) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. However, the instability of EtG in urine against bacterial degradation or the post-collectional synthesis of EtG in contaminated samples may cause false interpretation of EtG results in urine samples. This study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism taking place during growth of bacterial colonies...
July 15, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28705666/in-vitro-activity-of-ceftolozane-tazobactam-against-pseudomonas-aeruginosa-and-enterobacteriaceae-isolates-recovered-from-hospitalized-patients-in-germany
#3
Harald Seifert, Barbara Körber-Irrgang, Michael Kresken
To evaluate the activity of ceftolozane/tazobactam in comparison with other broad-spectrum antimicrobials against Pseudomonas aeruginosa and Enterobacteriaceae, 497 non-duplicate P. aeruginosa and 802 Enterobacteriaceae clinical isolates were consecutively collected during the period from September 2014 to April 2015 from patients in Germany with bloodstream, lower respiratory tract, intraabdominal or urinary tract infections. Antimicrobial susceptibility testing was performed by broth microdilution. Results were interpreted according to EUCAST criteria...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28683780/bacterial-agents-and-antibiotic-resistance-profiles-of-infections-from-different-sites-that-occurred-among-patients-at-debre-markos-referral-hospital-ethiopia-a-cross-sectional-study
#4
Wondemagegn Mulu, Bayeh Abera, Mulat Yimer, Tadesse Hailu, Haimanot Ayele, Dereje Abate
BACKGROUND: In developing countries like Ethiopia, infections with antibiotic resistant bacteria become a real threat. Hence, monitoring of local level antimicrobial resistance profile is indispensable to contain the spread of drug resistant bacteria and intervene poor awareness on antimicrobial resistance. Therefore, this study aimed at determining bacterial and antibiotic resistance profiles of infections from different sites that occurred among patients. METHODS: Retrospective data recorded were analyzed on culture and drug susceptibility test results at Debre Markos Referral Hospital which were performed from 2011 to 2014...
July 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28671150/emerging-trends-of-nosocomial-pneumonia-in-intensive-care-unit-of-a-tertiary-care-public-teaching-hospital-in-western-india
#5
Rakesh Bhadade, Minal Harde, Rosemarie deSouza, Ashwini More, Ramesh Bharmal
BACKGROUND: Nosocomial pneumonia poses great challenge to an intensivist. Detailed information about hospital-acquired pneumonia (HAP) and ventilator-acquired pneumonia (VAP) is crucial for prevention and optimal management, thus improving quality Intensive Care Unit (ICU) care. Hence, we aimed to study the current trend of nosocomial pneumonia in ICU. MATERIALS AND METHODS: It was a prospective observational cohort study, conducted in the ICU of a tertiary care teaching public hospital over a period of 18 months...
July 2017: Annals of African Medicine
https://www.readbyqxmd.com/read/28669814/quick-96fasp-for-high-throughput-quantitative-proteome-analysis
#6
Yanbao Yu, Shiferaw Bekele, Rembert Pieper
Filter aided sample preparation (FASP) is becoming a central method for proteomic sample cleanup and peptide generation prior to LC-MS analysis. We previously adapted this method to a 96-well filter plate, and applied to prepare protein digests from cell lysate and body fluid samples in a high throughput quantitative manner. While the 96FASP approach is scalable and can handle multiple samples simultaneously, two key advantages compared to single FASP, it is also time-consuming. The centrifugation-based liquid transfer on the filter plate takes 3-5 times longer than single filter...
June 29, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28668690/in-vivo-development-of-tigecycline-resistance-in-klebsiella-pneumoniae-due-to-deletion-of-ramr-ribosomal-binding-site
#7
Meiping Ye, Baixing Ding, Hongliang Qian, Qingqing Xu, Jianping Jiang, Jinwei Huang, Hongyu Ou, Fupin Hu, Minggui Wang
Tigecycline resistance is emerging among Klebsiella pneumoniae isolates, but knowledge on in vivo development of tigecycline resistance is limited. Here, we report a new mechanism of tigecycline resistance in K. pneumoniae which evolved during tigecycline therapy. K. pneumoniae isolates were consecutively obtained from urine samples of a patient with scrotal abscess and urinary tract infection before and during tigecycline treatment. Two tigecycline-resistant K. pneumoniae strains (KP-3R and KP-4R, MIC=8 µg/ml) were isolated after 41 and 47 days of tigecycline therapy...
June 28, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28663538/urinary-tract-infections-after-kidney-transplantation-a-risk-factor-analysis-of-417-patients
#8
Liselotte Ooms, Jan IJzermans, Anne Voor In 't Holt, Michiel Betjes, Margreet Vos, Turkan Terkivatan
BACKGROUND The aim of this study was to evaluate the number of urinary tract infections (UTI) that occur after kidney transplantation (KT) and to identify possible risk factors for development of a UTI. MATERIAL AND METHODS We retrospectively analyzed all KTs performed between January 2012 and December 2013 in the Erasmus University Medical Center, Rotterdam. UTI was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacterium of ≥10^5 CFU/mL, (3) which was treated with antibiotics, and (4) which occurred within 3 months after KT...
June 30, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28643188/impact-of-appropriateness-of-empiric-therapy-on-outcomes-in-community-onset-bacteremia-by-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-klebisella-pneumoniae-definitively-treated-with-carbapenems
#9
E-J Joo, D A Park, N R Lee, S-Y Moon, J-K Choi, J-H Ko, K R Peck
Despite a significant increase of bloodstream infection caused by extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae in the community-setting, information regarding clinical outcomes of inappropriate empiric therapy (IAT) in patients with those infections is limited. A multicenter-retrospective cohort study was conducted in four hospitals. A total of 249 adults were identified to have community-onset bacteremia caused by ESBL-producing Escherichia coli and Klebsiella pneumoniae, and definitively treated with carbapenems...
June 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28638594/high-burden-of-antimicrobial-resistance-among-gram-negative-bacteria-causing-healthcare-associated-infections-in-a-critical-care-unit-of-nepal
#10
Narayan Prasad Parajuli, Subhash Prasad Acharya, Shyam Kumar Mishra, Keshab Parajuli, Basista Prasad Rijal, Bharat Mani Pokhrel
BACKGROUND: Healthcare associated infections (HCAI) and antimicrobial resistance are principal threats to the patients of intensive care units and are the major determining factors for patient outcome. They are associated with increased morbidity, mortality, excess hospitalization and financial costs. The present study is an attempt to investigate the spectrum and antimicrobial resistance of bacterial isolates involved in healthcare associated infections (HCAI) in the patients of a critical care unit at a tertiary care university hospital in Kathmandu, Nepal...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28632788/high-throughput-identification-and-rational-design-of-synergistic-small-molecule-pairs-for-combating-and-bypassing-antibiotic-resistance
#11
Morgan A Wambaugh, Viplendra P S Shakya, Adam J Lewis, Matthew A Mulvey, Jessica C S Brown
Antibiotic-resistant infections kill approximately 23,000 people and cost $20,000,000,000 each year in the United States alone despite the widespread use of small-molecule antimicrobial combination therapy. Antibiotic combinations typically have an additive effect: the efficacy of the combination matches the sum of the efficacies of each antibiotic when used alone. Small molecules can also act synergistically when the efficacy of the combination is greater than the additive efficacy. However, synergistic combinations are rare and have been historically difficult to identify...
June 2017: PLoS Biology
https://www.readbyqxmd.com/read/28625025/-causative-bacterial-strains-and-sensitivityto-antimicrobial-agents-in-female-acute-uncomplicated-cystitis
#12
Takuya Owari, Tomoki Yamamoto, Shinichiro Mizobuchi, Yositaka Itami, Tomonori Nakahama, Yosihiro Matsumoto, Kaoru Yamada, Hitoshi Momose
Recently, wide spreading of fluoloquinolone resistant Escherichia coli is a serious problem inthe treatment of urinary tract infection. To investigate the causative bacterial strains of female acute uncomplicated cystitis (AUC) in the community and their sensitivity to antimicrobial agents, we retrospectively reviewed the medical records of 215 female AUC patients treated at our clinics from April 2014 to June 2015. Two hundred and nineteen strains were isolated as the causative bacteria from the patients'urine samples, including E...
May 2017: Hinyokika Kiyo. Acta Urologica Japonica
https://www.readbyqxmd.com/read/28621642/spectrum-and-antibiotic-resistance-of-uropathogens-between-2004-and-2015-in-a-tertiary-care-hospital-in-hungary
#13
András Magyar, Béla Köves, Károly Nagy, András Dobák, Vinodh Kumar Adithyaa Arthanareeswaran, Péter Bálint, Florian Wagenlehner, Péter Tenke
PURPOSE: Surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (UTIs), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time. METHODOLOGY: We retrospectively analysed all urine samples taken in the department of urology in a tertiary care hospital in Hungary from January 2004 to December 2015...
June 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28617330/identification-of-pathogenic-factors-in-klebsiella-pneumoniae-using-impedimetric-sensor-equipped-with-biomimetic-surfaces
#14
Duyen Thi Ngoc Huynh, Ah-Young Kim, Young-Rok Kim
K.pneumoniae is an opportunistic pathogen that causes nosocomial infections, such as, pneumonia, urinary tract infections, septic shock, and gastro intestinal disease. Lipopolysaccharide (LPS), capsular polysaccharide, and fimbriae are recognized major virulence factors of K.pneumoniae and play key roles during early stages of infections. In this study, we functionalized the surface of gold electrode with mannose and mucin to monitor the adhesion-associated virulence factors of K.pneumoniae. The binding characteristics of K...
June 15, 2017: Sensors
https://www.readbyqxmd.com/read/28607025/pharmacodynamic-evaluation-of-fosfomycin-against-e-coli-and-klebsiella-spp-from-urinary-tract-infections-and-the-influence-of-ph-on-fosfomycin-activities
#15
Nayara Helisandra Fedrigo, Josmar Mazucheli, James Albiero, Danielle Rosani Shinohara, Fernanda Gomes Lodi, Ana Cristina Dos Santos Machado, Sherwin K B Sy, Maria Cristina Bronharo Tognim
Fosfomycin is widely used for the treatment of uncomplicated urinary tract infection (UTI) and recently have been recommended for treating multidrug-resistant (MDR) gram-negative bacilli. Whether urine acidification can improve bacterial susceptibility to fosfomycin oral dosing regimen has not been analyzed. Minimal inhibitory concentration (MIC) was determined using agar dilution in pH 6.0 and 7.0 for 245 Gram negative bacterial isolates, consisting of 158 E. coli and 87 Klebsiella spp. which were collected from patients with UTI...
June 12, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28584733/nosocomial-infections-and-antibiotic-resistance-pattern-in-open-heart-surgery-patients-at-imam-ali-hospital-in-kermanshah-iran
#16
Fatemeh Heydarpour, Youssef Rahmani, Behzad Heydarpour, Atefeh Asadmobini
Background: Patients undergoing open heart surgery have a relatively high risk of acquiring nosocomial infections. The development of antibiotic-resistant infections is associated with prolonged hospital stays and mortalities. Objectives: The present study was conducted to investigate nosocomial infections and the antibiotic resistance pattern in bacteria causing these infections in open heart surgery patients at Imam Ali Hospital in Kermanshah in the west of Iran over a 4-year period from March 2011 to March 2014...
2017: GMS Hygiene and Infection Control
https://www.readbyqxmd.com/read/28584431/hospital-acquired-infection-prevalence-and-outcome-in-infants-undergoing-open-heart-surgery-in-the-present-era
#17
Manoj Kumar Sahu, Ch Bharat Siddharth, Velayudham Devagouru, Sachin Talwar, Sarvesh Pal Singh, Shiv Chaudhary, Balram Airan
BACKGROUND: The aim of this study is to evaluate the causal relation between hospital-acquired infection (HAI) and clinical outcomes following cardiac surgery in neonates and infants and to identify the risk factors for the development of HAI in this subset of patients. MATERIALS AND METHODS: After Ethics committee approval, one hundred consecutive infants undergoing open heart surgery (OHS) between June 2015 and June 2016 were included in this prospective observational study...
May 2017: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28580804/burden-of-antimicrobial-resistance-in-an-era-of-decreasing-susceptibility
#18
Glenn S Tillotson, Stephen H Zinner
Antimicrobial resistance has become a global problem. Many pathogens are becoming multidrug-resistant with the attendant increased risk of failure of standard therapies and the under-recognised outcomes such as increased morbidity, mortality, length of hospitalization and costs of treatment. Areas covered: We undertook a review of the literature using standard search engines including PubMed, Google Scholar, Scopus and internet sources. Key search terms included antimicrobial resistance, antibiotic resistance, bacterial resistance, clinical outcomes, economic consequences, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae and Staphylococcus aureus...
June 13, 2017: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/28571186/clinical-and-laboratory-profile-of-urinary-tract-infections-in-type-2-diabetics-aged-over-60-years
#19
Sadhna Sharma, Biju Govind, Sujeet Kumar Naidu, Srinivas Kinjarapu, Mohammed Rasool
INTRODUCTION: Urinary tract infections are frequently encountered among diabetic patients and the incidence rate increases with age. There have been growing research to identify the clinical profile of urinary tract infections in diabetic patients. However, such studies on elderly patients are rare. AIM: To determine the risk factors, clinical/laboratory profiles, causative organisms and antimicrobial susceptibilities in type 2 diabetics aged over 60 years. MATERIALS AND METHODS: This prospective single centre study was conducted at NRI Medical College and General Hospital, Guntur, India, between November 2012 and November 2014...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28561794/antibiotic-resistance-and-virulence-phenotypes-of-recent-bacterial-strains-isolated-from-urinary-tract-infections-in-elderly-patients-with-prostatic-disease
#20
Cristina Delcaru, Paulina Podgoreanu, Ionela Alexandru, Nela Popescu, Luminiţa Măruţescu, Coralia Bleotu, George Dan Mogoşanu, Mariana Carmen Chifiriuc, Marinela Gluck, Veronica Lazăr
Acute bacterial prostatitis is one of the frequent complications of urinary tract infection (UTI). From the approximately 10% of men having prostatitis, 7% experience a bacterial prostatitis. The purpose of this study was to investigate the prevalence of uropathogens associated with UTIs in older patients with benign prostatic hyperplasia and to assess their susceptibility to commonly prescribed antibiotics as well as the relationships between microbial virulence and resistance features. Uropathogenic Escherichia coli was found to be the most frequent bacterial strain isolated from patients with benign prostatic hyperplasia, followed by Enterococcus spp...
May 31, 2017: Pathogens
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