keyword
MENU ▼
Read by QxMD icon Read
search

Myocyte regeneration

keyword
https://www.readbyqxmd.com/read/28194397/a-novel-human-tissue-engineered-3-d-functional-vascularized-cardiac-muscle-construct
#1
Mani T Valarmathi, John W Fuseler, Jeffrey M Davis, Robert L Price
Organ tissue engineering, including cardiovascular tissues, has been an area of intense investigation. The major challenge to these approaches has been the inability to vascularize and perfuse the in vitro engineered tissue constructs. Attempts to provide oxygen and nutrients to the cells contained in the biomaterial constructs have had varying degrees of success. The aim of this current study is to develop a three-dimensional (3-D) model of vascularized cardiac tissue to examine the concurrent temporal and spatial regulation of cardiomyogenesis in the context of postnatal de novo vasculogenesis during stem cell cardiac regeneration...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28181511/natriuretic-peptide-receptor-b-modulates-the-proliferation-of-the-cardiac-cells-expressing-the-stem-cell-antigen-1
#2
Stéphanie Rignault-Clerc, Christelle Bielmann, Lucas Liaudet, Bernard Waeber, François Feihl, Nathalie Rosenblatt-Velin
Brain Natriuretic Peptide (BNP) injections in adult "healthy" or infarcted mice led to increased number of non-myocyte cells (NMCs) expressing the nuclear transcription factor Nkx2.5. The aim of this study was to identify the nature of the cells able to respond to BNP as well as the signaling pathway involved. BNP treatment of neonatal mouse NMCs stimulated Sca-1(+) cell proliferation. The Sca-1(+) cells were characterized as being a mixed cell population involving fibroblasts and multipotent precursor cells...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28163790/hypertrophy-changes-3d-shape-of-hipsc-cardiomyocytes-implications-for-cellular-maturation-in-regenerative-medicine
#3
Cassady E Rupert, Heidi H Chang, Kareen L K Coulombe
Advances in the use of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes for heart regeneration and in vitro disease models demand a greater understanding of how these cells grow and mature in 3-dimensional space. In this study, we developed an analysis methodology of single cardiomyocytes plated on 2D surfaces to assess their 3D myofilament volume and its z-height distribution, or shape, upon hypertrophic stimulation via phenylephrine (PE) treatment or long-term culture ("aging"). Cardiomyocytes were fixed and labeled with α-actinin for confocal microscopy imaging to obtain z-stacks for 3D myofilament volume analysis...
February 2017: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/28155919/obtaining-spontaneously-beating-cardiomyocyte-like-cells-from-adipose-derived-stromal-vascular-fractions-cultured-on-enzyme-crosslinked-gelatin-hydrogels
#4
Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Heart failure often develops after acute myocardial infarction because the injured myocardial tissue fails to recover or regenerate. Stem cell transplantation using adult cell sources, such as adipose-derived stromal vascular fraction (SVF), draws extensive attention. In this study, SVF cells were isolated from rat adipose tissue and cultivated on enzyme-crosslinked gelatin hydrogels. Morphological features of cell development and spontaneous beating behavior from these cells were observed and recorded. Cardiac phenotypes were characterized via immunofluorescence staining, and the expression of cardiac-specific genes was measured via RT-PCR...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28116799/in-vitro-models-of-the-cardiac-microenvironment-to-study-myocyte-and-non-myocyte-crosstalk-bioinspired-approaches-beyond-the-polystyrene-dish
#5
Celinda M Kofron, Ulrike Mende
The heart is a complex pluricellular organ comprised of cardiomyocytes and non-myocytes, including fibroblasts, endothelial cells, and immune cells. Myocytes are responsible for electrical conduction and contractile force generation, while the other cell types are responsible for matrix deposition, vascularization, and injury response. Myocytes and non-myocytes are known to communicate and exert mutual regulatory effects. In concert, they determine the structural, electrical, and mechanical characteristics in the healthy and remodeled myocardium...
January 23, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28104772/the-elusive-progenitor-cell-in-cardiac-regeneration-slip-slidin-away
#6
REVIEW
Chen-Leng Cai, Jeffery D Molkentin
The adult human heart is unable to regenerate after various forms of injury, suggesting that this organ lacks a biologically meaningful endogenous stem cell pool. However, injecting the infarcted area of the adult mammalian heart with exogenously prepared progenitor cells of various types has been reported to create new myocardium by the direct conversion of these progenitor cells into cardiomyocytes. These reports remain controversial because follow-up studies from independent laboratories failed to observe such an effect...
January 20, 2017: Circulation Research
https://www.readbyqxmd.com/read/28087623/a-p53-based-genetic-tracing-system-to-follow-postnatal-cardiomyocyte-expansion-in-heart-regeneration
#7
Qi Xiao, Guoxin Zhang, Huijuan Wang, Lai Chen, Shuangshuang Lu, Dejing Pan, Geng Liu, Zhongzhou Yang
For heart regeneration, the proliferative potential of cardiomyocytes in postnatal mice is under intense investigations. However, solely relying on immunostaining of proliferation markers, the long term proliferation dynamics and potential of the cardiomyocytes cannot be readily addressed. Previously, we found that a p53 promoter driving reporter predominantly marked the proliferating lineages in mice. Here, we established a p53 based genetic tracing system to investigate postnatal cardiomyocyte proliferation and heart regeneration...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28018900/redox-regulation-of-heart-regeneration-an-evolutionary-tradeoff
#8
Waleed M Elhelaly, Nicholas T Lam, Mohamed Hamza, Shuda Xia, Hesham A Sadek
Heart failure is a costly and deadly disease, affecting over 23 million patients worldwide, half of which die within 5 years of diagnosis. The pathophysiological basis of heart failure is the inability of the adult heart to regenerate lost or damaged myocardium. Although limited myocyte turnover does occur in the adult heart, it is insufficient for restoration of contractile function (Nadal-Ginard, 2001; Laflamme et al., 2002; Quaini et al., 2002; Hsieh et al., 2007; Bergmann et al., 2009, 2012). In contrast to lower vertebrates (Poss et al...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27977899/individual-evaluation-of-cardiac-marker-expression-and-self-beating-during-cardiac-differentiation-of-p19cl6-cells-on-different-culture-substrates
#9
Tetsuji Yamaoka, Mitsuhi Hirata, Takaaki Dan, Atsushi Yamashita, Akihisa Otaka, Takahiko Nakaoki, Azizi Miskon, Sachiro Kakinoki, Atsushi Mahara
Cell-based therapies using self-beating cardiomyocytes have been attracting great attention for use in cardiac regeneration, although an effective procedure to improve cardiac differentiation and self-beating induction is required. The purpose of this study is to clarify the effect of the culture substrate on cardiac maturation by separately evaluating the cardiac differentiation step and the beating induction step in vitro. To this end, the well-studied cardiomyocyte-like progenitor cell line P19CL6 and neonatal cardiomyocytes (NCMs) were selected and cultured on substrates coated with collagen type I (Col-I), gelatin (Gel), fibronectin (FN), or poly-L-lysine (PLL)...
December 15, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27967605/early-developmental-disruption-of-type-2-deiodinase-pathway-in-mouse-skeletal-muscle-does-not-impair-muscle-function
#10
Daniele L Ignacio, Diego H S Silvestre, Elena Anne-Palmer, Barbara M L C Bocco, Tatiana L Fonseca, Miriam O Ribeiro, Balázs Gereben, Antonio C Bianco, Joao P Werneck-de-Castro
BACKGROUND: Myogenesis is positively regulated by thyroid hormone (triiodothyronine [T3]), which is amplified by the type 2 deiodinase (D2) activation of thyroxine to T3. Global inactivation of the Dio2 gene impairs skeletal muscle (SKM) differentiation and regeneration in response to muscle injury. Given that newborn and adult mice with late developmental SKM Dio2 disruption do not develop a significant phenotype, it was hypothesized that D2 plays an early role in this process. METHODS: This was tested in mice with SKM disruption of Dio2 driven by two early developmental promoters: MYF5 and MYOD...
February 10, 2017: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/27943289/roles-of-notch1-signaling-in-regulating-satellite-cell-fates-choices-and-postnatal-skeletal-myogenesis
#11
REVIEW
Tizhong Shan, Ziye Xu, Weiche Wu, Jiaqi Liu, Yizhen Wang
Adult skeletal muscle stem cells, also called satellite cells, are indispensable for the growth, maintenance, and regeneration of the postnatal skeletal muscle. Satellite cells, predominantly quiescent in mature resting muscles, are activated after skeletal muscle injury or degeneration. Notch1 signaling is an evolutionarily conserved pathway that plays crucial roles in satellite cells homeostasis and postnatal skeletal myogenesis and regeneration. Activation of Notch1 signaling promotes the muscle satellite cells quiescence and proliferation, but inhibits differentiation of muscle satellite cells...
December 12, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27930666/development-of-pet-imaging-to-visualize-activated-macrophages-accumulated-in-the-transplanted-ipsc-derived-cardiac-myocytes-of-allogeneic-origin-for-detecting-the-immune-rejection-of-allogeneic-cell-transplants-in-mice
#12
Noriyuki Kashiyama, Shigeru Miyagawa, Satsuki Fukushima, Takuji Kawamura, Ai Kawamura, Shohei Yoshida, Akima Harada, Tadashi Watabe, Yasukazu Kanai, Koichi Toda, Jun Hatazawa, Yoshiki Sawa
Allogeneic transplantation (Tx) of induced pluripotent stem cells (iPSCs) is a promising tissue regeneration therapy. However, this inevitably induces macrophage-mediated immune response against the graft, limiting its therapeutic efficacy. Monitoring the magnitude of the immune response using imaging tools would be useful for prolonging graft survival and increasing the therapy longevity. Minimally invasive quantitative detection of activated macrophages by medical imaging technologies such as positron emission tomography (PET) imaging targets translocator protein (TSPO), which is highly expressed on mitochondrial membrane, especially in activated macrophage...
2016: PloS One
https://www.readbyqxmd.com/read/27844250/differential-response-of-human-cardiac-stem-cells-and-bone-marrow-mesenchymal-stem-cells-to-hypoxia-reoxygenation-injury
#13
Deepthi Sreerengam RajendranNair, Jayakumar Karunakaran, Renuka R Nair
Cardiosphere-derived cells (CDCs) and bone marrow mesenchymal stem cells (MSCs) are popularly used in stem cell therapy for myocardial regeneration. The cell type that survives and maintains stem cell characteristics in the adverse microenvironment following ischemia-reperfusion injury is presumed to be ideal for transplantation. The study was therefore aimed at identifying the cell type with relatively greater resistance to ischemia-reperfusion injury. CDCs were isolated from the right atrial appendage and MSCs from bone marrow of patients who underwent coronary artery bypass graft surgery...
January 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27809412/pgc-1%C3%AE-4-gene-expression-is-suppressed-by-the-il-6-mek-erk-1-2-mapk-signaling-axis-and-altered-by-resistance-exercise-obesity-and-muscle-injury
#14
Jacob L Brown, Megan E Rosa-Caldwell, David E Lee, Lemuel A Brown, Richard A Perry, Kevin L Shimkus, Thomas A Blackwell, James D Fluckey, James A Carson, Sami Dridi, Tyrone A Washington, Nicholas P Greene
AIM: PGC-1α4 is a novel regulator of muscle hypertrophy, however, there is limited understanding of the regulation of its expression and role in many (patho)physiologic conditions. Therefore, our purpose was to elicit signaling mechanisms regulating gene expression of Pgc1α4 and examine its response to (patho)physiologic stimuli associated with altered muscle mass. METHODS: IL-6 knockout mice and pharmacological experiments in C2C12 myocytes were used to identify regulation of Pgc1α4 transcription...
November 3, 2016: Acta Physiologica
https://www.readbyqxmd.com/read/27798352/mitogen-activated-protein-kinase-p38-target-regenerating-islet-derived-3%C3%AE-expression-is-upregulated-in-cardiac-inflammatory-response-in-the-rat-heart
#15
Hanna Säkkinen, Jani Aro, Leena Kaikkonen, Pauli Ohukainen, Juha Näpänkangas, Heikki Tokola, Heikki Ruskoaho, Jaana Rysä
Regenerating islet-derived 3γ (Reg3γ) is a multifunctional protein, associated with various tissue injuries and inflammatory states. Since chronic inflammation is characteristics also for heart failure, the aim of this study was to characterize Reg3γ expression in cardiac inflammatory conditions. Reg3γ expression was studied in experimental rat models of myocardial infarction (MI) and pressure overload in vivo. For cell culture studies neonatal rat cardiac myocytes (NRCMs) were used. In addition, adenovirus-mediated gene transfer of upstream mitogen-activated protein kinase (MAPK) kinase 3b and p38α MAPK in vivo and in vitro was performed...
October 2016: Physiological Reports
https://www.readbyqxmd.com/read/27796891/cardiac-telocytes-in-regeneration-of-myocardium-after-myocardial-infarction
#16
Liao Zhaofu, Cai Dongqing
Recent research progress has revealed that a novel type of interstitial cells termed cardiac telocytes (CTs) is found in the interstitium of the heart. We demonstrated that CTs are distributed both longitudinally and within the cross network in the myocardium and that the density of CTs in the atrium-atria and base of the myocardium is higher than that in the middle of the myocardium, while the density of CTs in the epicardium is higher than that in the endocardium. In addition, we documented, for the first time, that the network of CTs in the infarct zone of the myocardium is destroyed during myocardial infarction (MI)...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27790620/immune-modulation-of-cardiac-repair-and-regeneration-the-art-of-mending-broken-hearts
#17
REVIEW
Ivana Zlatanova, Cristina Pinto, Jean-Sébastien Silvestre
The accumulation of immune cells is among the earliest responses that manifest in the cardiac tissue after injury. Both innate and adaptive immunity coordinate distinct and mutually non-exclusive events governing cardiac repair, including elimination of the cellular debris, compensatory growth of the remaining cardiac tissue, activation of resident or circulating precursor cells, quantitative and qualitative modifications of the vascular network, and formation of a fibrotic scar. The present review summarizes the mounting evidence suggesting that the inflammatory response also guides the regenerative process following cardiac damage...
2016: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/27783047/camkk2-suppresses-muscle-regeneration-through-the-inhibition-of-myoblast-proliferation-and-differentiation
#18
Cheng Ye, Duo Zhang, Lei Zhao, Yan Li, Xiaohan Yao, Hui Wang, Shengjie Zhang, Wei Liu, Hongchao Cao, Shuxian Yu, Yucheng Wang, Jingjing Jiang, Hui Wang, Xihua Li, Hao Ying
Skeletal muscle has a major role in locomotion and muscle disorders are associated with poor regenerative efficiency. Therefore, a deeper understanding of muscle regeneration is needed to provide a new insight for new therapies. CaMKK2 plays a role in the calcium/calmodulin-dependent kinase cascade; however, its role in skeletal muscle remains unknown. Here, we found that CaMKK2 expression levels were altered under physiological and pathological conditions including postnatal myogensis, freeze or cardiotoxin-induced muscle regeneration, and Duchenne muscular dystrophy...
October 24, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27780890/novel-protective-effects-of-pulsed-electromagnetic-field-ischemia-reperfusion-injury-rats
#19
Fenfen Ma, Wenwen Li, Xinghui Li, Ba Hieu Tran, Rinkiko Suguro, Ruijuan Guan, Cuilan Hou, Huijuan Wang, Aijie Zhang, Yichun Zhu, YiZhun Zhu
Extracorporeal pulsed electromagnetic field (PEMF) has shown the ability to regenerate tissue by promoting cell proliferation. In the present study, we investigated for the first time whether PEMF treatment could improve the myocardial ischaemia/reperfusion (I/R) injury and uncovered its underlying mechanisms.In our study, we demonstrated for the first time that extracorporeal PEMF has a novel effect on myocardial I/R injury. The number and function of circulating endothelial progenitor cells (EPCs) were increased in PEMF treating rats...
December 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27780147/stem-cell-therapies-for-congenital-heart-disease
#20
REVIEW
Masoumeh Ghafarzadeh, Mehrdad Namdari, Ali Eatemadi
Congenital heart disease (CHD) is the most prevalent congenital anomaly in newborn babies. Cardiac malformations have been induced in different animal model experiments, by perturbing some molecules that take part in the developmental pathways associated with myocyte differentiation, specification, or cardiac morphogenesis. The exact epigenetic, environmental, or genetic, basis for these molecules perturbations is yet to be understood. But, scientist have bridged this gap by introducing autologous stem cell into the defective hearts to treat CHD...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
keyword
keyword
120375
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"