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https://www.readbyqxmd.com/read/29556382/characterization-of-human-dental-pulp-cells-grown-in-chemically-defined-serum-free-medium
#1
Sakiko Fujii, Katsumi Fujimoto, Noriko Goto, Yoshimitsu Abiko, Asayo Imaoka, Jinchang Shao, Kazuko Kitayama, Masami Kanawa, Agung Sosiawan, Ketut Suardita, Fusanori Nishimura, Yukio Kato
Dental pulp cells (DPCs) are promising candidates for use as transplantable cells in regenerative medicine. However, ex vivo expansion of these cells typically requires culture media containing fetal bovine serum, which may cause infection and immunological reaction following transplantation. In addition, the proliferation and differentiation of DPCs markedly depend upon serum batches. Therefore, the present study examined whether DPCs could be expanded under serum-free conditions. DPCs obtained from four donors were identified to proliferate actively in the serum-free medium, STK2, when compared with those cells in control medium (Dulbecco's modified Eagle's medium containing 10% serum)...
April 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29552452/early-electronic-screen-exposure-and-autistic-like-symptoms
#2
Donna Hermawati, Farid Agung Rahmadi, Tanjung Ayu Sumekar, Tri Indah Winarni
Prevalence autism spectrum disorders (ASD) has been on rise, but many studies suggests over-diagnosed. Currently, children have more access to electronic media on the daily basis than those of previous generation. Some studies suggest that increases screen time is associated with melanopsin-expressing neurons and decreasing gamma-aminobutyric acid (GABA) neurotransmitter, and thus results aberrant behavior, decreased cognitive, and language development. Early exposure of electronic media in early life (< 2 years old) gives an impact on language, but it still inconclusive...
February 2018: Intractable & Rare Diseases Research
https://www.readbyqxmd.com/read/29441323/the-ethanolic-extract-of-ashitaba-stem-angelica-keskei-miq-koidz-as-future-antituberculosis
#3
Sri Agung Fitri Kusuma, Yoppi Iskandar, Mutiara Ayu Dewanti
Considering the easy contagion of tuberculosis (TB) disease spread and the emergence of multidrug-resistant TB, which directly impacts the failure of therapeutic goals and mortality rates increasing, TB disease control remains to be the main concern of continuous health development effort. Therefore, the discovery of new TB drug is needed. This research assessed the new natural anti-TB drug from the ethanolic extract of Angelica keiskei stem obtained from Lombok, Indonesia. The objectives of this study were to evaluate the sensitivity of Mycobacterium tuberculosis (Mtb) H37Rv strain to A...
January 2018: Journal of Advanced Pharmaceutical Technology & Research
https://www.readbyqxmd.com/read/29354609/side-to-side-ileosigmoidostomy-shunting-surgery-for-the-treatment-of-elderly-patients-with-chronic-constipation
#4
Yuda Handaya, Agung Maryanto, Marijata
Constipation is a digestive disorder that often occurs in the elderly; its main cause is bowel motility disorder. Treatments for patients with chronic constipation include pharmacotherapy, diet changes, and surgery if other therapies do not offer satisfactory results. We describe 4 patients, 2 men (70 and 65 years old) and 2 women (75 and 66 years old), who were diagnosed with chronic constipation (slow transit constipation) and treated with conventional therapy, but did not improve. For that reason, side-to-side ileosigmoidostomy shunting surgery was performed...
December 2017: Annals of Coloproctology
https://www.readbyqxmd.com/read/29350579/evaluation-of-respiratory-motion-corrected-cone-beam-ct-at-end-expiration-in-abdominal-radiotherapy-sites-a-prospective-study
#5
Russell E Kincaid, Agung E Hertanto, Yu-Chi Hu, Abraham J Wu, Karyn A Goodman, Hai D Pham, Ellen D Yorke, Qinghui Zhang, Qing Chen, Gig S Mageras
BACKGROUND: Cone beam computed tomography (CBCT) for radiotherapy image guidance suffers from respiratory motion artifacts. This limits soft tissue visualization and localization accuracy, particularly in abdominal sites. We report on a prospective study of respiratory motion-corrected (RMC)-CBCT to evaluate its efficacy in localizing abdominal organs and improving soft tissue visibility at end expiration. MATERIAL AND METHODS: In an IRB approved study, 11 patients with gastroesophageal junction (GEJ) cancer and five with pancreatic cancer underwent a respiration-correlated CT (4DCT), a respiration-gated CBCT (G-CBCT) near end expiration and a one-minute free-breathing CBCT scan on a single treatment day...
January 19, 2018: Acta Oncologica
https://www.readbyqxmd.com/read/29326761/prevalence-rate-of-adolescent-idiopathic-scoliosis-results-of-school-based-screening-in-surabaya-indonesia
#6
I S Komang-Agung, S B Dwi-Purnomo, A Susilowati
Introduction: Scoliosis is a lateral spinal deformity of 10 degrees or more, resulting in a C-shaped or S-shaped curve of the spine. Information about adolescent idiopathic scoliosis (AIS) prevalence rate is important not only for paediatric health care planning strategy but also for parent's awareness. This study aims to find the suitable inclination cut-off angle and the prevalence rate of AIS in Surabaya, Indonesia. Materials and Methods: This is a descriptive cross-sectional study conducted in 2010. We performed stratified random sampling of 784 Elementary and Junior High School students in Surabaya between 9-16 years of age...
November 2017: Malaysian Orthopaedic Journal
https://www.readbyqxmd.com/read/29248210/results-of-an-international-comparison-of-activity-measurements-of-68-ge
#7
J T Cessna, R Fitzgerald, B E Zimmerman, L Laureano-Pérez, D E Bergeron, F van Wyngaardt, M Smith, T Jackson, B Howe, C J da Silva, A Iwahara, P A L da Cruz, M Zhang, H Liu, J Liang, C Fréchou, C Bobin, P Cassette, K Kossert, O Nähle, J Marganiec-Gałązka, L Joseph, A Ravindra, D N Kulkarni, A Yunoki, Y Sato, K B Lee, J M Lee, Agung, T Dziel, A Listkowska, Z Tymiński, M Sahagia, A Antohe, M-R Ioan, A Luca, M Krivosek, J Ometakova, A Javornik, M Zalesakova, E García-Toraño Martinez, M Roteta, M Mejuto, Y Nedjadi, F Juget, M-C Yuan, C Y Yeh, E Yeltepe, A Dirican, J Keightley, A Pearce
An international key comparison, identifier CCRI(II)-K2.Ge-68, has been performed. The National Institute of Standards and Technology (NIST) served as the pilot laboratory, distributing aliquots of a68 Ge/68 Ga solution. Results for the activity concentration, CA , of68 Ge at a reference date of 12h00 UTC 14 November 2014 were submitted by 17 laboratories, encompassing many variants of coincidence methods and liquid-scintillation counting methods. The first use of 4π(Cherenkov)β-γ coincidence and anticoincidence methods in an international comparison is reported...
April 2018: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29238241/predictors-of-mortality-within-prison-and-after-release-among-persons-living-with-hiv-in-indonesia
#8
Gabriel J Culbert, Forrest W Crawford, Astia Murni, Agung Waluyo, Alexander R Bazazi, Junaiti Sahar, Frederick L Altice
Objectives: HIV-related mortality is increasing in Indonesia, where prisons house many people living with HIV and addiction. We examined all-cause mortality in HIV-infected Indonesian prisoners within prison and up to 24 months post-release. Materials and Methods: Randomly selected HIV-infected male prisoners (n=102) from two prisons in Jakarta, Indonesia completed surveys in prison and were followed up for 2 years (until study completion) or until they died or were lost to follow-up...
2017: Research and Reports in Tropical Medicine
https://www.readbyqxmd.com/read/29224362/structure-determination-and-quantification-of-a-new-flavone-glycoside-with-anti-acetylcholinesterase-activity-from-the-herbs-of-elsholtzia-ciliata
#9
Agung Nugroho, Jong-Hyuk Park, Jae Sue Choi, Kyoung-Sik Park, Joon-Pyo Hong, Hee-Juhn Park
Three acacetin triglycosides (compounds 1, 2 and 3) were isolated from the herbs of Elsholtzia ciliata (Labiatae). The structure were identified as 7-O-β-D-glucopyranosyl-(1 → 2)[α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside (compound 1), 7-O-(6-O-acetyl)-β-D-glucopyranosyl-(1 → 2)[α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside (compound 2) and 7-O-(6-O-acetyl)-β-D-glucopyranosyl-(1 → 2)[(4-O-acetyl)-α-L-rhamnopyranosyl-(1 → 6)]-β-D-glucopyranoside (compound 3) of acacetin...
December 11, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29203099/effects-of-8-hydroxyisocapnolactone-2-3-diol-and-friedelin-on-mast-cell-degranulation
#10
Muhammad Novrizal Abdi Sahid, Agung Endro Nugroho, Ratna Asmah Susidarti, Kazutaka Maeyama
OBJECTIVE: To investigate the effects of friedelin (terpenoid) and 8-hydroxyisocapnolactone-2-3-diol (coumarin) with concentration 10 μM, 30 μM, and 100 μM on inhibiting mast cells (MCs) degranulation. METHODS: The investigation was performed in vitro by administering each compound into rat peritoneal MCs and rat basophilic leukemia-2H3 cells followed by activation with 50 μg/mL of compound 48/80 or 1 μM of ionomycin. The concentration of histamine released from each group was measured by a high-performance liquid chromatography-fluorometry system with post-column derivatization using o-phthalaldehyde...
November 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29101341/quantum-mechanics-as-classical-statistical-mechanics-with-an-ontic-extension-and-an-epistemic-restriction
#11
Agung Budiyono, Daniel Rohrlich
Where does quantum mechanics part ways with classical mechanics? How does quantum randomness differ fundamentally from classical randomness? We cannot fully explain how the theories differ until we can derive them within a single axiomatic framework, allowing an unambiguous account of how one theory is the limit of the other. Here we derive non-relativistic quantum mechanics and classical statistical mechanics within a common framework. The common axioms include conservation of average energy and conservation of probability current...
November 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/29068106/clinical-profile-of-congenital-rubella-syndrome-in-yogyakarta-indonesia
#12
Elisabeth S Herini, Gunadi, Agung Triono, Fita Wirastuti, Kristy Iskandar, Niprida Mardin, Yati Soenarto
BACKGROUND: Congenital rubella syndrome (CRS) has many severe neurological manifestations and other systemic consequences. Although various studies have been done in Indonesia, there are no conclusive results on CRS incidence. The aim of this study was therefore to investigate the incidence, clinical manifestations and outcomes of CRS in Yogyakarta, Indonesia. METHODS: A descriptive study involving a review of congenital anomalies associated with CRS was carried out at Dr Sardjito Hospital, Yogyakarta, Indonesia, from July 2008 to June 2013...
February 2018: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/28919828/jugular-bulb-oxygen-saturation-correlates-with-full-outline-of-responsiveness-score-in-severe-traumatic-brain-injury-patients
#13
Tjokorda Gde Agung Senapathi, Made Wiryana, Ketut Sinardja, Ketut Wibawa Nada, Ida Bagus Krisna Jaya Sutawan, Christopher Ryalino, Aloysius Alphonso
BACKGROUND: Maintaining brain oxygenation status is the main goal of treatment in severe traumatic brain injury (TBI). Jugular venous oxygen saturation (SjvO2) monitoring is a technique to estimate global balance between cerebral oxygen supply and its metabolic requirement. Full Outline of Responsiveness (FOUR) score, a new consciousness measurement scoring, is expected to become an alternative for Glasgow Coma Scale (GCS) in evaluating neurologic status of patients with severe traumatic head injury, especially for those under mechanical ventilation...
2017: Open Access Emergency Medicine: OAEM
https://www.readbyqxmd.com/read/28827562/transcriptional-control-of-motility-enables-directional-movement-of-escherichia-coli-in-a-signal-gradient
#14
Jayamary Divya Ravichandar, Adam G Bower, A Agung Julius, Cynthia H Collins
Manipulation of cellular motility using a target signal can facilitate the development of biosensors or microbe-powered biorobots. Here, we engineered signal-dependent motility in Escherichia coli via the transcriptional control of a key motility gene. Without manipulating chemotaxis, signal-dependent switching of motility, either on or off, led to population-level directional movement of cells up or down a signal gradient. We developed a mathematical model that captures the behaviour of the cells, enables identification of key parameters controlling system behaviour, and facilitates predictive-design of motility-based pattern formation...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28761368/ultrasound-guided-bilateral-superficial-cervical-plexus-block-is-more-effective-than-landmark-technique-for-reducing-pain-from-thyroidectomy
#15
Tjokorda Gde Agung Senapathi, I Made Gede Widnyana, I Gusti Ngurah Mahaalit Aribawa, Made Wiryana, I Ketut Sinardja, I Ketut Wibawa Nada, Aa Gde Putra Semara Jaya, I Gede Koko Swadharma Putra
PURPOSE: Thyroidectomy causes postoperative pain and patient discomfort. Bilateral superficial cervical plexus block is a regional anesthesia technique that can provide analgesia during and after surgery. This study aims to compare the effectiveness of ultrasound (US)-guided versus landmark (LM) technique for bilateral superficial cervical plexus block in thyroidectomy. PATIENTS AND METHODS: Thirty-six patients undergoing thyroidectomy were divided into two groups randomly (n=18); either US-guided (US group) or LM technique (LM group) for bilateral superficial cervical plexus block...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28752803/designing-and-collecting-data-for-a-longitudinal-study-the-sleman-health-and-demographic-surveillance-system-hdss
#16
Fatwa S T Dewi, Ifta Choiriyyah, Citra Indriyani, Abdul Wahab, Lutfan Lazuardi, Agung Nugroho, Susetyowati Susetyowati, Rosalia K Harisaputra, Risalia Santi, Septi K Lestari, Nawi Ng, Mohammad Hakimi, Hari K Josef, Adi Utarini
BACKGROUND: This paper describes the methodological considerations of developing an urban Health and Demographic Surveillance System (HDSS), in the Sleman District of Yogyakarta, Indonesia. METHODS: 1) The Sleman District was selected because it is mostly an urban area. 2) The minimum sample size was calculated to measure infant mortality as the key variable and resulted in a sample of 4942 households. A two-stage cluster sampling procedure with probability proportionate to size was applied; first, 216 Censuses Blocks (CBs) were selected, and second, 25 households in each CB were selected...
July 1, 2017: Scandinavian Journal of Public Health
https://www.readbyqxmd.com/read/28695925/anti-inflammatory-effects-of-an-ethanol-extract-of-aster-glehni-via-inhibition-of-nf-%C3%AE%C2%BAb-activation-in-mice-with-dss-induced-colitis
#17
Jun-Hyeok Choi, Kyung-Sook Chung, Bo-Ram Jin, Se-Yun Cheon, Agung Nugroho, Seong-Soo Roh, Hyo-Jin An
Although Aster glehni has been reported to prevent diabetes mellitus, hypercholesterolemia, insomnia, and cardiovascular disease, the anti-inflammatory effect of Aster glehni in colonic tissue remains unclear. In this study, we investigated the anti-inflammatory effects and the underlying molecular mechanism of an ethanol extract of Aster glehni (AG) in mice with dextran sulfate sodium (DSS)-induced colitis. AG significantly attenuated DSS-induced DAI scores, which implied that it suppressed diarrhea, gross bleeding, and the infiltration of immune cells...
July 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28658964/products-of-chickpea-processing-as-texture-improvers-in-gluten-free-bread
#18
Libby G Bird, Casey L Pilkington, Agung Saputra, Luca Serventi
Recent market developments raised the need for alternatives to hydrocolloids as texture improver in gluten-free bread. Chickpea exerts several physicochemical properties (water- and oil-binding, emulsifying and foaming) that might address this need. Therefore, the effect of processing on chickpea functionality was tested on low ingredient dose, comparably to that of common hydrocolloids. Control bread was small, hard and with low gas retention ability as shown by microscopy, depicting holes inside crumb pores...
December 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28645761/beach-macro-litter-monitoring-and-floating-microplastic-in-a-coastal-area-of-indonesia
#19
Agung Dhamar Syakti, Rafika Bouhroum, Nuning Vita Hidayati, Chandra Joei Koenawan, Abdelaziz Boulkamh, Isdy Sulistyo, Stephanie Lebarillier, Syafsir Akhlus, Pierre Doumenq, Pascal Wong-Wah-Chung
Qualitative analysis of the structures of the polymers composing floating plastic debris was performed using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and the aging of the debris was assessed by measuring carbonyl group formation on the particle surfaces. Plastic material made up >75% of the 2313 items collected during a three-year survey. The size, shape and color of the microplastic were correlated with the polymer structure. The most abundant plastic materials were polypropylene (68%) and low-density polyethylene (11%), and the predominant colors of the plastics were white, blue and green...
September 15, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28645725/cell-surface-n-glycan-alteration-in-hepad38-cell-lines-expressing-hepatitis-b-virus
#20
Surya Agung Priyambada, Ryo Misaki, Toru Okamoto, Yuta Okamoto, Takao Ohashi, Keiji Ueda, Yoshiharu Matsuura, Kazuhito Fujiyama
Hepatitis B virus (HBV) is the smallest partially double-stranded DNA virus known to infect humans. Worldwide, more than 50% of hepatocellular carcinoma (HCC) cases are related to chronic Hepatitis B. Development of HCC from normal liver cells is characterized by changes in cell surface N-glycans, which can promote the invasive behavior of tumor cells, leading ultimately to the progression of cancer. However, little is understood about the cell surface N-glycans of HBV-infected liver cells. We try to address this by taking advantage of the HepAD38 cell line, which can replicate HBV in the absence of tetracycline [tet(-)] in growth medium...
June 20, 2017: Virus Research
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