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Autologous Regenerative

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https://www.readbyqxmd.com/read/28333339/platelet-rich-plasma-augments-adipose-derived-stem-cell-growth-and-differentiation
#1
Robert P Gersch, Joshua Glahn, Michael G Tecce, Anthony J Wilson, Ivona Percec
Background: Adipose-derived stem cells (ASCs) are a powerful tool for cosmetic surgery and regenerative medicine. The use of autologous platelet rich plasma (PRP), particularly in combination with ASC-based therapy, has significantly expanded in recent years. Unfortunately, the mechanisms and optimal dosing responsible for the beneficial effects of PRP remain poorly understood. Here we investigate the effect of PRP on ASC growth and differentiation. Objectives: To assess the impact of different PRP feeding and cryopreservation protocols on ASC isolation, expansion, and differentiation...
February 22, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28327848/-mesenchymal-stem-cells-definitions-culture-and-potential-applications
#2
Willy Ceron, Iván Lozada-Requena, Kiomi Ventocilla, Sandra Jara, Milagros Pinto, Marco Cabello, José L Aguilar
In recent years, mesenchymal stem cells (MSC) have become very important due to their high plasticity and their ability to release paracrine factors able to interact with various cell types, tissues and organs. The use of MSC in regenerative medicine became of vital importance, since they do not express histocompatibility MHC molecules class II nor costimulant molecules, and low expression of MHC class I, will not be rejected by individuals of same species, they could be used in an autologous, and eventually, allogeneic manner...
October 2016: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28326683/restoring-full-thickness-defects-with-spray-skin-in-conjunction-with-dermal-regenerate-template-and-split-thickness-skin-grafting-a-pilot-study
#3
Daniel Hammer, Juan L Rendon, Jennifer Sabino, Kerry Latham, Mark E Fleming, Ian L Valerio
Complex, full-thickness soft tissue defects secondary to large burns, trauma and war-related injuries continue to challenge reconstructive surgeons. To achieve positive surgical outcomes in these patient populations, novel approaches are needed to restore the functional, protective and aesthetic properties of skin. Herein, we provide the first report describing the staged use of a dermal regenerate template (DRT) with a spray-on epidermal regenerative modality (spray skin) in addition to autologous split-thickness skin grafting (STSG) in non-burn trauma and compare these results with those of patients treated with DRT and STSG alone...
March 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28326305/implications-for-a-stem-cell-regenerative-medicine-based-approach-to-human-intervertebral-disk-degeneration
#4
REVIEW
Petra Kraus, Thomas Lufkin
The human body develops from a single cell, the zygote, the product of the maternal oocyte and the paternal spermatozoon. That 1-cell zygote embryo will divide and eventually grow into an adult human which is comprised of ~3.7 × 10(13) cells. The tens of trillions of cells in the adult human can be classified into approximately 200 different highly specialized cell types that make up all of the different tissues and organs of the human body. Regenerative medicine aims to replace or restore dysfunctional cells, tissues and organs with fully functional ones...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28323545/creation-of-a-bioengineered-skin-flap-scaffold-with-a-perfusable-vascular-pedicle
#5
Bernhard J Jank, Jeremy Goverman, Jacques P Guyette, Jon M Charest, Mark A Randolph, Glenn R Gaudette, Joshua R Gershlak, Martin Purschke, Emilia Javorsky, Rosalynn M Nazarian, David A Leonard, Curtis L Cetrulo, William G Austen, Harald Christian Ott
Full thickness skin loss is a challenging problem due to limited reconstructive options, demanding 75 million surgical procedures annually in the United States. Autologous skin grafting is the gold standard treatment, but results in donor site morbidity and poor aesthetics. Numerous skin substitutes are available on the market to date, however, none truly function as full thickness skin due to their lack of a vascular network. The creation of an autologous full thickness skin analogue with a vascular pedicle would result in a paradigm shift in the management of wounds and in reconstruction of full thickness skin defects...
March 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28300208/practical-whole-tooth-restoration-utilizing-autologous-bioengineered-tooth-germ-transplantation-in-a-postnatal-canine-model
#6
Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamaguchi, Takashi Tsuji, Takuo Kuboki
Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28296613/autologous-induced-stem-cell-derived-retinal-cells-for-macular-degeneration
#7
Michiko Mandai, Akira Watanabe, Yasuo Kurimoto, Yasuhiko Hirami, Chikako Morinaga, Takashi Daimon, Masashi Fujihara, Hiroshi Akimaru, Noriko Sakai, Yumiko Shibata, Motoki Terada, Yui Nomiya, Shigeki Tanishima, Masahiro Nakamura, Hiroyuki Kamao, Sunao Sugita, Akishi Onishi, Tomoko Ito, Kanako Fujita, Shin Kawamata, Masahiro J Go, Chikara Shinohara, Ken-Ichiro Hata, Masanori Sawada, Midori Yamamoto, Sachiko Ohta, Yasuo Ohara, Kenichi Yoshida, Junko Kuwahara, Yuko Kitano, Naoki Amano, Masafumi Umekage, Fumiyo Kitaoka, Azusa Tanaka, Chihiro Okada, Naoko Takasu, Seishi Ogawa, Shinya Yamanaka, Masayo Takahashi
We assessed the feasibility of transplanting a sheet of retinal pigment epithelial (RPE) cells differentiated from induced pluripotent stem cells (iPSCs) in a patient with neovascular age-related macular degeneration. The iPSCs were generated from skin fibroblasts obtained from two patients with advanced neovascular age-related macular degeneration and were differentiated into RPE cells. The RPE cells and the iPSCs from which they were derived were subject to extensive testing. A surgery that included the removal of the neovascular membrane and transplantation of the autologous iPSC-derived RPE cell sheet under the retina was performed in one of the patients...
March 16, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28296571/efficient-generation-of-functional-schwann-cells-from-adipose-derived-stem-cells-in-defined-conditions
#8
Songtao Xie, Fan Lu, Juntao Han, Ke Tao, Hongtao Wang, Alfred Simental, Dahai Hu, Hao Yang
Schwann cells (SCs) are hitherto regarded as the most promising candidates for viable cell-based therapy to peripheral nervous system (PNS) injuries or degenerative diseases. However, the extreme drawbacks of transplanting autologous SCs for clinical applications still represent a significant bottleneck in neural regenerative medicine, mainly owing to the need of sacrificing a functional nerve to generate autologous SCs and the nature of slow expansion of the SCs. Thus, it is of great importance to establish an alternative cell system for the generation of sufficient SCs...
March 15, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28293168/modeling-human-neurological-and-neurodegenerative-diseases-from-induced-pluripotent-stem-cells-to-neuronal-differentiation-and-its-applications-in-neurotrauma
#9
REVIEW
Hisham Bahmad, Ola Hadadeh, Farah Chamaa, Katia Cheaito, Batoul Darwish, Ahmad-Kareem Makkawi, Wassim Abou-Kheir
With the help of several inducing factors, somatic cells can be reprogrammed to become induced pluripotent stem cell (iPSCs) lines. The success is in obtaining iPSCs almost identical to embryonic stem cells (ESCs), therefore various approaches have been tested and ultimately several ones have succeeded. The importance of these cells is in how they serve as models to unveil the molecular pathways and mechanisms underlying several human diseases, and also in its potential roles in the development of regenerative medicine...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28289528/clinical-cardiac-regenerative-studies-in-children
#10
REVIEW
Imre J Pavo, Ina Michel-Behnke
Although the incidence of pediatric heart failure is low, the mortality is relatively high, with severe clinical symptoms requiring repeated hospitalization or intensive care treatment in the surviving patients. Cardiac biopsy specimens have revealed a higher number of resident human cardiac progenitor cells, with greater proliferation and differentiation capacity, in the neonatal period as compared with adults, demonstrating the regeneration potential of the young heart, with rising interest in cardiac regeneration therapy in critically ill pediatric patients...
February 26, 2017: World Journal of Cardiology
https://www.readbyqxmd.com/read/28286618/molecular-and-cellular-interactions-of-allogenic-and-autologus-mesenchymal-stem-cells-with-innate-and-acquired-immunity-and-their-role-in-regenerative-medicine
#11
REVIEW
Roghayeh Hosseinikia, Mohammad Reza Nikbakht, Ali Asghar Moghaddam, Ahmad Tajehmiri, Mahboobe Hosseinikia, Farhad Oubari, Mahin Nikougoftar Zarif, Yahya Pasdar, Kamran Mansouri
Mesenchymal stem cells (MSCs), as major stem cells for cell therapy, have been studied from different aspects in preclinical and clinical settings for more than a decade. These cells modulate the immune system (humoral and cellular immune responses) in vitro by producing soluble factors (anti-inflammatory molecules) and/or making cell-cell contacts. Hence, they could be used in regenerative medicine, tissue engineering and immune therapy. MSCs-based therapy have been recently used for treatment of cancer regarding the migratory potential of these cells towards tumor cells which makes them considerable candidates, also for cell therapy in both allogeneic and autologous settings...
January 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
https://www.readbyqxmd.com/read/28280722/tissue-engineered-solutions-in-plastic-and-reconstructive-surgery-principles-and-practice
#12
REVIEW
Sarah Al-Himdani, Zita M Jessop, Ayesha Al-Sabah, Emman Combellack, Amel Ibrahim, Shareen H Doak, Andrew M Hart, Charles W Archer, Catherine A Thornton, Iain S Whitaker
Recent advances in microsurgery, imaging, and transplantation have led to significant refinements in autologous reconstructive options; however, the morbidity of donor sites remains. This would be eliminated by successful clinical translation of tissue-engineered solutions into surgical practice. Plastic surgeons are uniquely placed to be intrinsically involved in the research and development of laboratory engineered tissues and their subsequent use. In this article, we present an overview of the field of tissue engineering, with the practicing plastic surgeon in mind...
2017: Frontiers in Surgery
https://www.readbyqxmd.com/read/28258665/ppar-%C3%AE-agonist-with-mesenchymal-stem-cells-induces-type-ii-collagen-producing-chondrocytes-in-human-arthritic-synovial-fluid
#13
Bruce E Heck, Joshua J Park, Vishruti Makani, Eun-Cheol Kim, Dong Hyun Kim
Osteoarthritis (OA) is an inflammatory joint disease characterized by degeneration of articular cartilage within synovial joints. An estimated 27 million Americans suffer from OA and the population is expected to reach 67 million in USA by 2030. Thus, it is urgent to find an effective OA treatment. Traditional OA treatments have no disease-modifying effect while regenerative OA therapies such as autologous chondrocyte implantation (ACI) show some promise. Nonetheless, current regenerative therapies do not overcome synovial inflammation that suppresses the differentiation of mesenchymal stem cells (MSCs) to chondrocytes and the expression of type II collagen, the major constituent of functional cartilage...
March 3, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28244829/regenerative-capacity-of-autologous-stem-cell-transplantation-in-elderly-a-report-of-biomedical-outcomes
#14
Maria Teresa Gonzalez-Garza, Delia Elva Cruz-Vega
The occurrence of chronic diseases such as neurological, metabolic and cardiovascular degenerative disorders increases with age. Cell therapy is an emerging approach to the treatment of these conditions. Of particular interest is the application of autologous stem cells because it eliminates post-transplantation immune rejection and there are less ethical concerns associated with their use. The regenerative capacity of stem cells harvested from elderly people is however controversial. In this review, we analyze if self-renewal potential, differentiation capability and expression of stemness genes in stem cells collected from elderly patients validate their application in clinical trials and examine the results...
March 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28242034/mesenchymal-stem-cell-derived-extracellular-vesicles-attenuate-kidney-inflammation
#15
Alfonso Eirin, Xiang-Yang Zhu, Amrutesh S Puranik, Hui Tang, Kelly A McGurren, Andre J van Wijnen, Amir Lerman, Lilach O Lerman
Mesenchymal stem/stromal cells (MSCs) have distinct capability for renal repair, but may have safety concerns. MSC-derived extracellular vesicles emerged as a novel noncellular alternative. Using a porcine model of metabolic syndrome and renal artery stenosis we tested whether extracellular vesicles attenuate renal inflammation, and if this capacity is mediated by their cargo of the anti-inflammatory cytokine interleukin (IL) 10. Pigs with metabolic syndrome were studied after 16 weeks of renal artery stenosis untreated or treated four weeks earlier with a single intrarenal delivery of extracellular vesicles harvested from adipose tissue-derived autologous MSCs...
February 24, 2017: Kidney International
https://www.readbyqxmd.com/read/28235633/cell-therapy-for-heart-disease-after-15-years-unmet-expectations
#16
REVIEW
Patrizia Nigro, Beatrice Bassetti, Laura Cavallotti, Valentina Catto, Corrado Carbucicchio, Giulio Pompilio
Over the past two decades cardiac cell therapy (CCT) has emerged as a promising new strategy to cure heart diseases at high unmet need. Thousands of patients have entered clinical trials for acute or chronic heart conditions testing different cell types, including autologous or allogeneic bone marrow (BM)-derived mononuclear or selected cells, BM- or adipose tissue-derived mesenchymal cells, or cardiac resident progenitors based on their potential ability to regenerate scarred or dysfunctional myocardium. Nowadays, the original enthusiasm surrounding the regenerative medicine field has been cushioned by a cumulative body of evidence indicating an inefficient or modest efficacy of CCT in improving cardiac function, along with the continued lack of indisputable proof for long-term prognostic benefit...
February 21, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28230922/adipose-derived-aldehyde-dehydrogenase-expressing-cells-promote-dermal-regenerative-potential-with-collagen-glycosaminoglycan-scaffold
#17
Hajime Matsumine, Kazuyuki Numakura, Satoshi Tsunoda, Huan Wang, Rui Matsumine, Mihail Climov, Giorgio Giatsidis, Vikas P Sukhatme, Dennis P Orgill
Aldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and highly expressed in stem cells. This study isolated ALDH-expressing cells from subcutaneous adipose tissue and investigated their potential to enhance healing in a full-thickness skin wound in rats by co-implanting them with collagen-glycosaminoglycan (c-GAG) scaffolds. ALDH-positive cells were isolated by a fluorescence-activated cell sorting technique from Lewis rat's stromal-vascular-fraction (SVF) and transplanted with c-GAG scaffolds in a rat full-thickness skin wound model...
November 7, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28224037/tooth-extraction-in-a-patient-after-autologous-skeletal-myoblast-sheet-transplantation-for-severe-dilated-cardiomyopathy
#18
Kohei Okuyama, Yuki Sakamoto, Tomofumi Naruse, Souichi Yanamoto, Masahiro Umeda
The authors describe the first case of tooth extraction in a patient treated with autologous skeletal myocardial sheet transplantation for severe dilated cardiomyopathy. This condition is associated with significant morbidity and mortality that may have identifiable causes or may be idiopathic or inherited. In addition to local disinfection, antimicrobial prophylaxis and continuous anticoagulant therapy during the perioperative period of tooth extraction were needed to prevent infective endocarditis and ensure the success of regenerative therapy of the heart...
March 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28217369/regenerative-medicine-using-dental-pulp-stem-cells-for-liver-diseases
#19
EDITORIAL
Shogo Ohkoshi, Hajime Hara, Haruka Hirono, Kazuhiko Watanabe, Katsuhiko Hasegawa
Acute liver failure is a refractory disease and its prognosis, if not treated using liver transplantation, is extremely poor. It is a good candidate for regenerative medicine, where stem cell-based therapies play a central role. Mesenchymal stem cells (MSCs) are known to differentiate into multiple cell lineages including hepatocytes. Autologous cell transplant without any foreign gene induction is feasible using MSCs, thereby avoiding possible risks of tumorigenesis and immune rejection. Dental pulp also contains an MSC population that differentiates into hepatocytes...
February 6, 2017: World Journal of Gastrointestinal Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28216604/evaluation-of-not-activated-and-activated-prp-in-hair-loss-treatment-role-of-growth-factor-and-cytokine-concentrations-obtained-by-different-collection-systems
#20
Pietro Gentile, John P Cole, Megan A Cole, Simone Garcovich, Alessandra Bielli, Maria Giovanna Scioli, Augusto Orlandi, Chiara Insalaco, Valerio Cervelli
Platelet rich plasma (PRP) was tested as a potential therapy for androgenetic alopecia (AGA) through two different clinical protocols in which one population (18 participants) received half-head treatment with autologous non-activated PRP (A-PRP) produced by CPunT Preparation System (Biomed Device, Modena, Italy) and the other half-head with placebo, and a second separated population in which all participants (n = 6, 3 participants per group) received treatment with calcium-activated PRP (AA-PRP) produced from one of two different PRP collection devices (Regen Blood Cell Therapy or Arthrex Angel System)...
February 14, 2017: International Journal of Molecular Sciences
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