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Chicken postovulatory follicle

Xin Lin, Xingting Liu, Yanfen Ma, Yuling Mi, Weidong Zeng, Jian Li, Caiqiao Zhang
After ovulation in mammals, rupture of mature follicles is reorganized into the corpus luteum that secrets progesterone (P4 ) to stimulate endometrial development. The situation in birds differs considerably. Beyond ovulation the ruptured avian follicle forms a postovulatory follicle (POF) that is not considered analogous to mammalian corpus luteum. The function and regression mechanisms of avian POFs remain poorly understood. Here we investigated the changes in apoptotic and autophagic activities that were involved during POF degradation...
April 29, 2018: Aging
Anna Hrabia, Joanna K Socha, Andrzej Sechman
The study was undertaken to examine mRNA expression and localization of selected matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs), and the activity of MMPs in chicken postovulatory follicles (POFs) during their apoptotic regression. Apoptotic cells and apoptosis-related caspase expression and activity were examined as well. Chickens were sacrificed 2 h and 21 h after ovulation, and five POFs (POF1 to POF5) were isolated from the ovaries. It was found that the number of apoptotic cells (TUNEL-positive) increased along with follicle regression...
May 1, 2018: General and Comparative Endocrinology
Zhenjie Yuan, Yuxia Chen, Qiuyue Chen, Miao Guo, Li Kang, Guiyu Zhu, Yunliang Jiang
Extracelluar matrix undergoes constant remodeling, cell-cell, and cell-matrix interactions during chicken ovarian follicle growth, which is coordinated by matrix metalloproteinases (MMPs), and their associated endogenous inhibitors (TIMPs). Transcriptome analysis revealed upregulation of MMP13 in sexually mature chicken ovaries. In this study, we found that the expression of MMP13 in chicken ovary was stably elevated from 60 d to 159 d, and was significantly higher at 159 d than at the other three developmental stages (P < 0...
May 3, 2016: G3: Genes—Genomes—Genetics
Guiyu Zhu, Li Kang, Qingqing Wei, Xinxing Cui, Shouzhi Wang, Yuxia Chen, Yunliang Jiang
Matrix metalloproteinases (MMPs) are a specific class of proteolytic enzymes that play critical roles in follicular development and luteinization in mammals. However, the role of MMPs in avian ovary remains largely unknown. We found that three MMP genes (MMP1, MMP3, and MMP9) were significantly up-regulated in 23-wk-old (laying phase) chicken ovaries compared with 6-wk-old ovaries (prepubertal phase). In reproductively active chicken ovary, MMP1 expression (both mRNA and protein) remained low in prehierarchical and preovulatory follicles but increased in postovulatory follicles (POFs)...
March 2014: Biology of Reproduction
Agnieszka Leśniak, Anna Hrabia
In the mammalian ovary, matrix metalloproteinase-2 (MMP-2) is thought to be involved in the regulated turnover of the extracellular matrix during the process of follicle development, ovulation and regression. Information concerning the role of MMP-2 in the chicken ovary is scarce. The present investigation was undertaken to determine the distribution of MMP-2 mRNA within the laying hen ovary as well as to define whether the expression of MMP-2 mRNA changes during the ovulatory cycle. Expression of MMP-2 mRNA in ovarian compartments was determined by RT-PCR analysis at two stages of the ovulatory cycle, i...
2012: Folia Biologica (Krakow)
Anna Hrabia, Helena Paczoska-Eliasiewicz, Józef Niezgoda, Janusz Rzasa
The study was undertaken to determine the effect of histamine on blood flow to the ovary and oviduct in the domestic hen (Gallus domesticus). Cardiac output and blood flow were measured with 86RbCl through the ovarian stroma, white ovarian follicles, yellow preovulatory follicles, postovulatory follicles and four oviductal parts: infundibulum, magnum, isthmus and shell gland 1 min and 5 min after histamine treatment. In comparison with control hens which received 0.9% NaCl, histamine significantly increased (by 21...
2005: Folia Biologica (Krakow)
N R Sundaresan, M D Marcus Leo, J Subramani, D Anish, M Sudhagar, K A Ahmed, M Saxena, J S Tyagi, K V H Sastry, V K Saxena
Melatonin (N-acetyl-5-methoxytryptamine), an indole hormone, regulates various biological functions through three different receptor subtypes (Mel-1a, Mel-1b, and Mel-1c). However, the distribution of different melatonin receptor subtypes in chicken reproductive tissues was not known. In the present investigation, the partial sequences of ovarian melatonin receptor subtypes (Mel-1a, Mel-1b, and Mel-1c) were characterized. Further, the expression profile of melatonin receptor subtypes in the granulosa and theca layers of different preovulatory and postovulatory follicles (POF) were studied by semi-quantitative RT-PCR...
January 2009: Veterinary Research Communications
Dale Buchanan Hales, Yan Zhuge, Jo Ann Jaen Lagman, Kristine Ansenberger, Cassandra Mahon, Animesh Barua, Judith L Luborsky, Janice M Bahr
Cyclooxygenase (COX) (PTGS) is the rate-limiting enzyme in the biosynthesis of prostaglandins. Two COX isoforms have been identified, COX-1 and COX-2, which show distinct cell-specific expression and regulation. Ovarian cancer is the most lethal gynecological malignancy and the disease is poorly understood due to the lack of suitable animal models. The laying hen spontaneously develops epithelial ovarian cancer with few or no symptoms until the cancer has progresses to a late stage, similar to the human disease...
June 2008: Endocrine
Sebastien Elis, Florence Batellier, Isabelle Couty, Sandrine Balzergue, Marie-Laure Martin-Magniette, Philippe Monget, Elisabeth Blesbois, Marina S Govoroun
BACKGROUND: The initial stages of development depend on mRNA and proteins accumulated in the oocyte, and during these stages, certain genes are essential for fertilization, first cleavage and embryonic genome activation. The aim of this study was first to search for avian oocyte-specific genes using an in silico and a microarray approaches, then to investigate the temporal and spatial dynamics of the expression of some of these genes during follicular maturation and early embryogenesis...
2008: BMC Genomics
N R Sundaresan, V K Saxena, K V H Sastry, K Nagarajan, Preeti Jain, Rani Singh, D Anish, P V Ravindra, M Saxena, K A Ahmed
The mechanism of postovulatory follicle (POF) regression in birds is still poorly understood. In the current study, expression of IL-1beta, IL-6, GM-CSF, IFN-gamma, IL-2, IL-4, IL-13, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IL-10 and TGF-beta2 mRNAs was estimated in regressing POF by semi-quantitative RT-PCR. In addition, the changes in immune cell population, histological and apoptotic changes were also studied in regressing POF. The expression of cytokines (IL-1beta, IL-6, IL-10 and TGF-beta2) and chemokines (chCXCLi2, chCCLi2, chCCLi4 and chCCLi7) was upregulated in POFs, suggesting a role for these molecules in tissue regression...
2008: Developmental and Comparative Immunology
N R Sundaresan, V K Saxena, K V H Sastry, D Anish, M D Marcus Leo, C Kantaraja, M Saxena, K A Ahmed
Chicken postovulatory follicle (POF) regression occurs via the process of apoptosis. However, the signals and initiator pathways responsible for regression of the POF are unknown. In the current study, we examined gene expression patterns of various caspases (caspase-1, -2 and -3) involved in apoptosis by semi-quantitative RT-PCR. The percentage of apoptotic cells during POF regression was also quantified by flow cytometry. Expression of caspase-3 mRNA was noted in the largest preovulatory follicle (F1). However, the initiator caspases (caspase-1 and -2) were not expressed in F1...
January 2008: Veterinary Research Communications
N R Sundaresan, V K Saxena, K V H Sastry, D Anish, M Saxena, K Nagarajan, K A Ahmed
Nitric oxide (NO) has recently emerged as a regulator of functional and structural regression in mammalian reproductive tissues. However, the role of NO in ovulation and postovulatory follicles (POF) that undergo regression in laying birds is unclear. In the present investigation, the expression profiles of iNOS mRNA, tissue NO levels and the percentage of apoptotic cells were studied in the regressing chicken postovulatory follicle (POF). The postovulatory follicles gradually lost weight during its regression and reached the lowest weight on POF5...
October 2007: Animal Reproduction Science
Kei Miyakoshi, Melinda J Murphy, Richard R Yeoman, Siddhartha Mitra, Christopher J Dubay, Jon D Hennebold
Proteolytic activities are essential for follicular growth, ovulation, as well as for luteal formation and regression. Using suppression subtractive hybridization (SSH), a novel mouse ovary-selective gene (termed protease serine 35, Prss35) was identified. Analysis of the mouse genome database using the Prss35 sequence led to the identification of a homologous protease (protease serine 23, Prss23). PRSS35 possesses general features that are characteristic of serine (Ser) proteases, but is unique in that the canonical Ser that defines this enzyme family is replaced by a threonine (Thr)...
December 2006: Biology of Reproduction
William J Murdoch, Edward A Van Kirk, Brenda M Alexander
A cause-effect relationship between ovulation and common (surface) epithelial ovarian cancer has been suspected for many years. The ovarian surface epithelium apparently becomes exposed to genotoxins that are generated during the ovulatory process. Intensive egg-laying hens readily develop ovarian carcinomatosis. Indeed, elevated levels of potentially mutagenic 8-oxo-guanine adducts were detected in avian ovarian epithelial cells isolated from the apical surfaces and perimeters of pre-and postovulatory follicles, respectively...
June 2005: Experimental Biology and Medicine
T M Lovell, R T Gladwell, N P Groome, P G Knight
To study the potential involvement of inhibin A (inhA), inhibin B (inhB), activin A (actA) and follistatin (FS) in the recruitment of follicles into the preovulatory hierarchy, growing follicles (ranging from 1 mm to the largest designated F1) and the three most recent postovulatory follicles (POFs) were recovered from laying hens (n=11). With the exception of <4 mm follicles and POFs, follicle walls were dissected into separate granulosa (G) and theca (T) layers before extraction. Contents of inhA, inhB, actA and FS in tissue extracts were assayed using specific two-site ELISAs and results are expressed per mg DNA...
April 2003: Journal of Endocrinology
A L Johnson, E V Solovieva, J T Bridgham
The present studies were conducted to address cellular mechanisms responsible for regulating steroidogenic acute regulatory protein (StAR) expression and progesterone synthesis at maturational stages corresponding to both the time of hen follicle selection, as well as before and after the LH surge in preovulatory follicle granulosa cells. A recently published report has established that mitogen-activated protein (MAP) kinase signaling induced by transforming growth factor alpha (TGFalpha) treatment blocks FSH-induced differentiation and StAR expression in cultured hen granulosa cells, whereas inhibitors of MAP kinase signaling enhance FSH-induced differentiation...
October 2002: Biology of Reproduction
J Ahn, S You, H Kim, D N Foster, M E El Halawani
We isolated cDNA encoding turkey inhibin-alpha (tINH-alpha) and -betaA (tINH-betaA) subunits from the turkey ovary using reverse transcription-polymerase chain reaction (RT-PCR). The isolated alpha subunit and betaA subunit included the entire open reading frames encoding 329 and 424 amino acids, respectively. The amino acid sequences of mature tINH-alpha subunit and tINH-betaA subunit (12.6 and 12.9 kDa proteins, respectively), established via DNA sequence analysis, were highly conserved between the chicken and various mammals...
December 2001: Poultry Science
J T Bridgham, A L Johnson
It is now well established that vertebrate ovarian follicles undergo atresia via apoptosis, a process that is initiated within the granulosa cell layer of undifferentiated follicles. Although the exact signals, membrane-bound receptors, and associated intracellular signaling pathways leading to apoptosis within granulosa cells have yet to be established, it is evident that multiple and redundant pathways exist. Fas, together with its ligand, has been the most commonly studied death-inducer in the mammalian ovary; however, nothing is currently known regarding expression of either Fas or the related tumor necrosis factor receptor type 1 (TNFR1), in avian species...
September 2001: Biology of Reproduction
A Barua, H Michiue, Y Yoshimura
The aim of this study was to determine the changes in the population of major histocompatibility complex class II positive (MHC-II(+)) cells in ovarian follicles during the processes of follicular growth, postovulatory regression and follicular atresia in hens. Cryostat sections of ovarian stroma containing cortical follicles, small white follicles, the largest (F(1)) and third largest (F(3)) preovulatory follicles, postovulatory and atretic follicles of laying hens were prepared. The sections were immunostained for MHC-II molecules using mouse anti-chicken MHC-II monoclonal antibody and positive cells were counted using a computer-assisted image analyser under a light microscope...
June 2001: Reproduction: the Official Journal of the Society for the Study of Fertility
T M Lovell, P G Knight, N P Groome, R T Gladwell
Inhibins and activins are firmly implicated in the control of pituitary FSH secretion and ovarian follicular development in mammals. As in mammals, inhibin A and activin A are expressed in the preovulatory follicles of birds, and a defined ovulation cycle for inhibin A has recently been demonstrated in the laying hen. To investigate further the role of inhibin-related proteins in developing pullets, circulating concentrations of inhibin A, inhibin B, total immunoreactive inhibin alpha-subunit (ir-alpha), activin A, LH, FSH, and progesterone were measured from the juvenile state through to sexual maturity in 22 birds...
January 2001: Biology of Reproduction
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