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https://www.readbyqxmd.com/read/28109145/-short-term-use-of-rapamycin-combined-with-tregs-prolongs-mouse-cardiac-graft-survival-but-attenuates-recipient-s-anti-tumor-immunity
#1
Ting Yan, Chen Luo, Xianmo Yang, Lei Ji, Shiqiao Luo
Objective To study the effect of short-term use of rapamycin(Rap) combined with regulatory T cells (Tregs) on the long-time survival of allogeneic mouse cardiac transplant, and its impact on the anti-tumor immunity of recipient. MethodsMouse Tregs were purified from recipients' spleen by magnetic activated cell sorting (MACS), and expanded by CD3/CD28 monoclonal antibody immunomagnetic beads and 2000 U/mL recombinant mouse IL-2 (rmIL-2) ex vivo. The purity was tested by fluorescence-activated cell sorting (FACS)...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#2
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28107450/systemic-t-cells-immunosuppression-of-glioma-stem-cell-derived-exosomes-is-mediated-by-monocytic-myeloid-derived-suppressor-cells
#3
Rossana Domenis, Daniela Cesselli, Barbara Toffoletto, Evgenia Bourkoula, Federica Caponnetto, Ivana Manini, Antonio Paolo Beltrami, Tamara Ius, Miran Skrap, Carla Di Loreto, Giorgia Gri
A major contributing factor to glioma development and progression is its ability to evade the immune system. Nano-meter sized vesicles, exosomes, secreted by glioma-stem cells (GSC) can act as mediators of intercellular communication to promote tumor immune escape. Here, we investigated the immunomodulatory properties of GCS-derived exosomes on different peripheral immune cell populations. Healthy donor peripheral blood mononuclear cells (PBMCs) stimulated with anti-CD3, anti-CD28 and IL-2, were treated with GSC-derived exosomes...
2017: PloS One
https://www.readbyqxmd.com/read/28107215/metabolic-cardiac-and-renal-effects-of-the-slow-hydrogen-sulfide-releasing-molecule-gyy4137-during-resuscitated-septic-shock-in-swine-with-pre-existing-coronary-artery-disease
#4
Benedikt L Nubaum, Josef Vogt, Ulrich Wachter, Oscar McCook, Martin Wepler, José Matallo, Enrico Calzia, Michael Gröger, Michael Georgieff, Mark E Wood, Matthew Whiteman, Peter Radermacher, Sebastian Hafner
Decreased levels of endogenous hydrogen sulfide (H2S) contribute to atherosclerosis, whereas equivocal data are available on H2S effects during sepsis. Moreover, H2S improved glucose utilization in anaesthetized, ventilated, hypothermic mice, but normothermia and/or sepsis blunted this effect. The metabolic effects of H2S in large animals are controversial. Therefore, we investigated the effects of the H2S donor GYY4137 during resuscitated, fecal peritonitis-induced septic shock in swine with genetically and diet-induced coronary artery disease (CAD)...
January 19, 2017: Shock
https://www.readbyqxmd.com/read/28106939/maximizing-output-in-rna-programmed-peptidyl-transfer-reactions
#5
Margherita Di Pisa, Anett Hauser, Oliver Seitz
A chemical reaction that is triggered by specific RNA molecules may provide opportunities for the design of artificial feedback loops. We envision a peptidyl transfer reaction, in which mRNA encoding for antiapoptotic protein would instruct the synthesis of apoptosis-inducing peptides. In this study, we tackled the RNA-programmed synthesis of a 16mer peptide derived from the Bak-BH3 protein which inhibits the antiapoptotic protein Bcl-xL. The reaction involves the transfer of a thioester-linked donor peptide fragment from one PNA conjugate onto an acceptor peptide-PNA conjugate...
January 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28105978/simultaneous-transplantation-of-fetal-ventral-mesencephalic-tissue-and-encapsulated-genetically-modified-cells-releasing-gdnf-in-a-hemi-parkinsonian-rat-model-of-parkinson-s-disease
#6
Alberto Perez-Bouza, Stefano Di, Stefanie Seiler, Morten Meyer, Lukas Andereggen, Alexander Huber, Raphael Guzman, Hans R
Transplantation of fetal ventral mesencephalic (VM) neurons for Parkinson's disease (PD) is limited by poor survival and suboptimal integration of grafted tissue into the host brain. In a 6-OHDA rat model of PD we investigated the feasibility of simultaneous transplantation of rat fetal VM tissue and polymer-encapsulated C2C12 myoblasts genetically modified to produce glial cell line-derived neurotrophic factor or mocktransfected myoblasts on graft function. Amphetamine-induced rotations were assessed prior and 2, 4, 6 and 9 weeks post-transplantation...
January 20, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28103989/-experimental-research-on-the-effects-of-different-activators-on-the-formation-of-platelet-rich-gel-and-the-release-of-bioactive-substances-in-human-platelet-rich-plasma
#7
Y Yang, W Zhang, B Cheng
Objective: To explore the effects of calcium gluconate and thrombin on the formation of platelet-rich gel (PRG) and the release of bioactive substances in human platelet-rich plasma (PRP) and the clinical significance. Methods: Six healthy blood donors who met the inclusion criteria were recruited in our unit from May to August in 2016. Platelet samples of each donor were collected for preparation of PRP. (1) PRP in the volume of 10 mL was collected from each donor and divided into thrombin activation group (TA, added with 0...
January 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28103692/phenotypic-screen-for-cardiac-regeneration-identifies-molecules-with-differential-activity-in-human-epicardium-derived-cells-versus-cardiac-fibroblasts
#8
Amalia I Paunovic, Lauren Drowley, Anneli Nordqvist, Elke Ericson, Elizabeth Mouchet, Anna Jonebring, Gunnar Grönberg, Alexander J Kvist, Ola Engkvist, Martin R Brown, Karin Gedda, Marie-José Goumans, Qing-Dong Wang, Alleyn T Plowright
Activation and proliferation of resident cardiac progenitor cells has therapeutic potential to repair the heart after injury. However, research has been impeded by a lack of well-defined and characterized cell sources and difficulties in translation to screening platforms. Here, we describe the development, validation, and use of a 384-well phenotypic assay in primary human epicardium-derived cells (EPDCs) to identify compounds that induce proliferation while maintaining the progenitor phenotype. Using this assay, we screened 7400 structurally diverse compounds where greater than 90% are biologically annotated and known to modulate a broad range of biological targets...
January 20, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28103425/efficient-synthesis-of-%C3%AE-galactosyl-oligosaccharides-using-a-mutant-bacteroides-thetaiotaomicron-retaining-%C3%AE-galactosidase-btgh97b
#9
Masayuki Okuyama, Kana Matsunaga, Ken-Ichi Watanabe, Keitaro Yamashita, Takayoshi Tagami, Asako Kikuchi, Min Ma, Patcharapa Klahan, Haruhide Mori, Min Yao, Atsuo Kimura
The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages. However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors. Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors...
January 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28102039/a-dispermic-chimerism-detected-in-a-taiwanese-potential-unrelated-hematopoietic-stem-cell-donor
#10
E K L Yang, S G E Marsh, P-Y Chen, C-P Chen, S-P Chen, P Y Lin
Chimerism is defined as the presence of 2 or more than 1 genetically distinct cell populations in an organism. Dispermic chimeras are derived from the fertilization of 1 or 2 matured nuclei by 2 sperms. We here report detection of a healthy and phenotypically normal female with normal ABO red blood cell typing in whom dispermic chimerism was suspected after 3 alleles were identified at multiple human leukocyte antigen (HLA) loci using molecular HLA analysis. Molecular HLA typing showed the donor to have 3 HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 alleles in blood, saliva and nail samples...
February 2017: HLA
https://www.readbyqxmd.com/read/28101950/molecular-binding-of-toxic-phenothiazinium-derivatives-azures-to-bovine-serum-albumin-a-comparative-spectroscopic-calorimetric-and-in-silico-study
#11
Somnath Das, Md Maidul Islam, Gopal Chandra Jana, Anirudha Patra, Pradeep K Jha, Maidul Hossain
In this paper, the comparative binding behavior of antimalarial drug azure A, azure B and azure C with bovine serum albumin (BSA) has been studied. The interaction has been confirmed by multispectroscopic (UV, fluorescence, Fourier transform infrared (FT-IR), and circular dichroism) and molecular docking techniques. The experimental results show that azure B has the highest BSA binding affinity followed by azure A and azure C. The experimental evidence of binding showed a static quenching mechanism in the interaction azures with BSA...
January 19, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/28099402/transplant-center-volume-and-the-risk-of-pancreas-allograft-failure
#12
Tarek Alhamad, Andrew F Malone, Daniel C Brennan, Robert J Stratta, Su-Hsin Chang, Jason R Wellen, Timothy A Horwedel, Krista L Lentine
BACKGROUND: Successful pancreas transplantation requires surgical expertise and multidisciplinary medical management. The impact of transplant center volume on pancreas allograft survival remains unclear. METHODS: We examined Organ Procurement and Transplantation Network (OPTN) data on 11,568 simultaneous pancreas-kidney (SPK) and 4308 solitary pancreas (pancreas transplant alone and pancreas after kidney) transplants between 2000 and 2013. RESULTS: Average annual transplant center volume was categorized by tertiles into low, medium and high volume, respectively, as follows: 1-6 (n=3,861), 7-13 (n=3,891), and 14-34 (n=3,888) for SPK, and 1-3 (n=1,417), 4-10 (n=1,518), and 11-33 (n=1,377) for solitary pancreas transplants...
January 17, 2017: Transplantation
https://www.readbyqxmd.com/read/28099204/narrowing-in-on-the-anti-%C3%AE-cell-specific-t-cells-looking-where-the-action-is
#13
Sally C Kent, Jenny Aurielle B Babon
PURPOSE OF THE REVIEW: By necessity, the vast majority of information we have on autoreactive T cells in human type 1 diabetes (T1D) has come from the study of peripheral blood of donors with T1D. It is not clear how representative the peripheral autoreactive T-cell repertoire is of the autoreactive T cells infiltrating the islets in T1D. We will summarize and discuss what is known of the immunohistopathology of insulitis, the T-cell receptor repertoire expressed by islet-infiltrating T cells, and the autoreactivity and function of islet-infiltrating T cells in T1D...
January 17, 2017: Current Opinion in Endocrinology, Diabetes, and Obesity
https://www.readbyqxmd.com/read/28098286/tridentate-lewis-acids-based-on-triphenylsilane
#14
Janek Tomaschautzky, Beate Neumann, Hans-Georg Stammler, Andreas Mix, Norbert W Mitzel
Several derivatives of the propeller-shaped ortho-substituted triphenylsilanes, carrying metal- or silicon-based acceptor groups, are reported. They were synthesized starting from tris(2-bromophenyl)fluorosilane, tris(2-vinylphenyl)fluorosilane and tris(2-ethynylphenyl)fluorosilane to generate a scope of Lewis-acidic molecules with different cavities. An improved synthetic protocol for donor-free tris(2-lithiophenyl)silanes is described. First attempts in host-guest chemistry to probe the binding between a threefold alane-functionalised ortho-substituted triphenylsilane and a tridentate Lewis-basic guest molecule are presented...
January 18, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28094830/molecular-electron-acceptors-for-efficient-fullerene-free-organic-solar-cells
#15
Shuixing Li, Zhongqiang Zhang, Minmin Shi, Chang-Zhi Li, Hongzheng Chen
Nowadays, organic solar cells (OSCs) with efficiencies over 10% have been achieved through the elaborate design of electron donors and fullerene acceptors. However, the drawbacks of fullerene acceptors, like poor absorption, limited chemical and energetic tunabilities, high-cost purification and morphological instability, have become the bottlenecks for the further improvement of OSCs. To overcome the mentioned shortages from fullerene, research studies on non-fullerene electron acceptors have boomed. To date, the highest efficiency of fullerene-free OSCs has been pushed to be 12%, which surpasses that of fullerene-based OSCs...
January 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28094779/suppression-of-colitis-by-adoptive-transfer-of-helminth-antigen-treated-dendritic-cells-requires-interleukin-4-receptor-%C3%AE-signaling
#16
C E Matisz, B Faz-López, E Thomson, A Al Rajabi, F Lopes, L I Terrazas, A Wang, K A Sharkey, D M McKay
Infection with helminth parasites has been explored as a treatment for autoimmune and inflammatory diseases. As helminth antigens have potent immunomodulation properties capable of inducing regulatory programs in a variety of cell types, transferring cells treated with helminth antigens represents a novel extension to helminth therapy. Previous work determined that transfer of bone marrow-derived dendritic cells (DC) pulsed with a crude extract of the tapeworm Hymenolepis diminuta (HD) can suppress colitis in recipient mice...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28094502/highly-efficient-full-color-thermally-activated-delayed-fluorescent-organic-light-emitting-diodes-extremely-low-efficiency-roll-off-utilizing-a-host-with-small-singlet-triplet-splitting
#17
Dongdong Zhang, Chongguang Zhao, Yunge Zhang, Xiaozeng Song, Pengcheng Wei, Minghan Cai, Lian Duan
Numerous efforts have been devoted to boost the efficiency of thermally activated delayed fluorescence (TADF) devices; however, strategies to suppress the device efficiency roll-off are still in urgent need. Here, a general and effective approach to suppress the efficiency roll-off of TADF devices is proposed, that is utilizing TADF materials as the hosts for TADF emitters. Bearing small singlet-triplet splitting (ΔEST) with donor and acceptor units, TADF materials as the hosts possess the potential to achieve matched frontier energy levels with the adjacent transporting layers, facilitating balanced charge injection, as well as bipolar charge transport mobilities, beneficial to the balanced charges transportation...
January 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28094376/cyclopentadithiophene-organic-core-in-small-molecule-organic-solar-cells-morphological-control-of-carrier-recombination
#18
Rocío Domínguez, Núria F Montcada, Pilar de la Cruz, Emilio Palomares, Fernando Langa
Two new planar and symmetrical A-D-A (electron acceptor-electron donor-electron acceptor) small molecules based on a commercial cyclopentadithiophene derivative have been synthesized for solution processed small molecule organic solar cells. The aim was to synthesise the molecules to be energetically identical (similar HOMO-LUMO energy levels) in order to assign the differences observed to changes in the film morphology or to differences in the interfacial recombination kinetics or both. Devices were electrically characterized under one sun simulated (1...
January 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28093899/crucial-role-of-donor-density-in-the-performance-of-oxynitride-perovskite-latio2n-for-photocatalytic-water-oxidation-reaction
#19
Feng-Qiang Xiong, Lipeng Wan, Yue Li, Tiju Thomas, Francis Joseph DiSalvo, Minghui Yang
LaTiO2N photocatalysts were prepared via thermal ammonalysis of flux-synthesized La2Ti2O7 and La2TiO5, and were investigated for water oxidation. Though LaTiO2N derived from La2TiO5 appears defect-free in UV-Vis-NIR and electron paramagnetic resonance spectroscopies, its performance is much lower than conventional La2Ti2O7-derived LaTiO2N with defects. It is shown by Mott-Schottky analysis that La2TiO5-derived LaTiO2N has significantly lower donor density; this can result in insufficient build-in electric field for the separation of photogenerated electrons and holes...
January 17, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28092668/upregulation-of-ret-induces-perineurial-invasion-of-pancreatic-adenocarcinoma
#20
M Amit, S Na'ara, L Leider-Trejo, Y Binenbaum, N Kulish, E Fridman, A Shabtai-Orbach, R J Wong, Z Gil
Tumor spread along nerves, a phenomenon known as perineurial invasion, is common in various cancers including pancreatic ductal adenocarcinoma (PDAC). Neural invasion is associated with poor outcome, yet its mechanism remains unclear. Using the transgenic Pdx-1-Cre/KrasG12D /p53R172H (KPC) mouse model, we investigated the mechanism of neural invasion in PDAC. To detect tissue-specific factors that influence neural invasion by cancer cells, we characterized the perineurial microenvironment using a series of bone marrow transplantation (BMT) experiments in transgenic mice expressing single mutations in the Cx3cr1, GDNF and CCR2 genes...
January 16, 2017: Oncogene
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