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https://www.readbyqxmd.com/read/28453746/activation-of-cecr1-in-m2-like-tams-promotes-paracrine-stimulation-mediated-glial-tumor-progression
#1
Changbin Zhu, Dana Mustafa, Ping-Pin Zheng, Marcel van der Weiden, Andrea Sacchetti, Maarten Brandt, Ihsan Chrifi, Dennie Tempel, Pieter J M Leenen, Dirk Jan Duncker, Caroline Cheng, Johan M Kros
Background.: The majority of glioma-associated microglia/macrophages have been identified as M2-type macrophages with immune suppressive and tumor supportive action. Recently, the extracellular adenosine deaminase protein Cat Eye Syndrome Critical Region Protein 1 (CECR1) was shown to regulate macrophage maturation. In this study, we investigate the role of CECR1 in the regulation of the glioma-associated macrophage response. Methods.: Expression of CECR1 was assessed in human glioma samples...
May 1, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28453717/reaching-the-global-target-to-reduce-stunting-an-investment-framework
#2
Meera Shekar, Jakub Kakietek, Mary R D'Alimonte, Hilary E Rogers, Julia Dayton Eberwein, Jon Kweku Akuoku, Audrey Pereira, Shan Soe-Lin, Robert Hecht
Childhood stunting, being short for one's age, has life-long consequences for health, human capital and economic growth. Being stunted in early childhood is associated with slower cognitive development, reduced schooling attainment and adult incomes decreased by 5-53%. The World Health Assembly has endorsed global nutrition targets including one to reduce the number of stunted children under five by 40% by 2025. The target has been included in the Sustainable Development Goals (SDG target 2.2). This paper estimates the cost of achieving this target and develops scenarios for generating the necessary financing...
June 1, 2017: Health Policy and Planning
https://www.readbyqxmd.com/read/28448877/synthesis-structure-and-cytotoxicity-of-a-series-of-dioxidomolybdenum-vi-complexes-featuring-salan-ligands
#3
Satabdi Roy, Monalisa Mohanty, Sagarika Pasayat, Sudarshana Majumder, Kulanthaivel Senthilguru, Indranil Banerjee, Martin Reichelt, Hans Reuter, Ekkehard Sinn, Rupam Dinda
Seven hexacoordinated cis-dioxidomolybdenum(VI) complexes [MoO2L(1-7)] (1-7) derived from various tetradentate diamino bis(phenolato) "salan" ligands, N,N'-dimethyl-N,N'-bis-(2-hydroxy-3-X-5-Y-6-Z-benzyl)-1,2-diaminoethane {(X=Br, Y=Me, Z=H (H2L(1)); X=Me, YCl, Z=H (H2L(2)); X=(i)Pr, Y=Cl, Z=Me (H2L(3))} and N,N'-bis-(2-hydroxy-3-X-5-Y-6-Z-benzyl)-1,2-diaminopropane {(X=Y=(t)Bu, Z=H (H2L(4)); X=Y=Me, Z=H (H2L(5)); X=(i)Pr, YCl, Z=Me (H2L(6)); X=Y=Br, Z=H (H2L(7))} containing O-N donor atoms, have been isolated and structurally characterized...
April 18, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28448587/collection-of-cell-free-dna-for-genomic-analysis-of-solid-tumors-in-a-clinical-laboratory-setting
#4
Christopher K Raymond, Jennifer Hernandez, Reynold Karr, Kay Hill, Mark Li
The breadth of diagnostic procedures that utilize cell free DNA (cfDNA) from human plasma has increased dramatically in recent years. Here, we confirm that tumor-derived cfDNA fragments are similar in size distribution to cfDNA derived from normal tissues. Therefore, collection procedures optimized with healthy donor specimens are likely to be applicable to the diagnosis and monitoring of many different cancer types. We verify that the distribution and DNA sequences of fragmentation sites in cfDNA from both normal-germline and tumor-derived cfDNA are non-random...
2017: PloS One
https://www.readbyqxmd.com/read/28447575/adipose-derived-mesenchymal-stem-cells-from-the-elderly-exhibit-decreased-migration-and-differentiation-abilities-with-senescent-properties
#5
Meichen Liu, Hua Lei, Ping Dong, Xin Fu, Zhigang Yang, Ying Yang, Jiguang Ma, Xia Liu, Yilin Cao, Ran Xiao
Adipose-derived stem cells (ADSCs) can be applied extensively in the clinic because they can be easily isolated and cause less donor-site morbidity; however, their application can be complicated by patient-specific factors, such as age and harvest site. In this study, we systematically evaluated the effects of age on the quantity and quality of hADSCs isolated from excised chest subcutaneous adipose tissue and investigated the underlying molecular mechanism. hADSCs were isolated from donors of three different age groups (i...
April 26, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28446235/differential-expression-of-cell-cycle-and-wnt-pathway-related-genes-accounts-for-differences-in-the-growth-and-differentiation-potential-of-wharton-s-jelly-and-bone-marrow-derived-mesenchymal-stem-cells
#6
Aristea K Batsali, Charalampos Pontikoglou, Dimitrios Koutroulakis, Konstantia I Pavlaki, Athina Damianaki, Irene Mavroudi, Kalliopi Alpantaki, Elisavet Kouvidi, George Kontakis, Helen A Papadaki
BACKGROUND: In view of the current interest in exploring the clinical use of mesenchymal stem cells (MSCs) from different sources, we performed a side-by-side comparison of the biological properties of MSCs isolated from the Wharton's jelly (WJ), the most abundant MSC source in umbilical cord, with bone marrow (BM)-MSCs, the most extensively studied MSC population. METHODS: MSCs were isolated and expanded from BM aspirates of hematologically healthy donors (n = 18) and from the WJ of full-term neonates (n = 18)...
April 26, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28445410/c-c-coupling-reactions-between-benzofurazan-derivatives-and-1-3-diaminobenzenes
#7
Gabriele Micheletti, Silvia Bordoni, Elena Chugunova, Carla Boga
Aromatic substitution reactions between 1,3-diaminobenzene and chloronitrobenzofurazan derivatives have never been reported so far. The aim of the current study was to synthesize novel electron-donor and -acceptor architectures of interest in applied fields and to provide new insights on the nucleophilic behavior of 1,3-diaminobenzenes. The reaction of 1,3-dipiperidinyl-, 1,3-dimorpholinyl-, 1,3-dipyrrolidinyl-, or 1,3-dimethylamino-benzene with 7-chloro-4,6-dinitrobenzofuroxan or with a series of chloro-nitrobenzofurazans has been carried out in mild conditions...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28443932/nucleoside-linked-shunt-products-in-the-reaction-catalyzed-by-the-class-c-radical-s-adenosylmethionine-methyltransferase-nosn
#8
Wei Ding, Yujie Wu, Xinjian Ji, Haocheng Qianzhu, Fener Chen, Zixin Deng, Yi Yu, Qi Zhang
NosN is a class C radical S-adenosylmethionine (SAM) methyltransferase (RSMT) involved in the biosynthesis of nosiheptide, a clinically interesting thiopeptide antibiotic produced by Streptomyces actuosus. NosN employs an unprecedented catalytic mechanism, in which SAM is converted to 5'-methylthioadenosine (MTA) as a direct methyl donor. In this study, we report identification of several nucleoside-linked shunt products in the NosN-catalyzed reaction. Comparative analysis of NosN and the class A RSMT RlmN and further density functional theory (DFT) calculations reveal important mechanistic insights into the catalyses of the two types of enzymes, showing that the radical intermediates generated by similar pathways can have very diverse reactivities...
April 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28443864/planar-chiral-ferrocenylphosphine-borane-complexes-featuring-agostic-type-b-he-e-hg-sn-interactions
#9
Alain C Tagne Kuate, Roger A Lalancette, F Jäkle
The synthesis of ferrocenylphosphine-borane adducts 1,2-fc(E)(PPh2·BH3) (E = SnR2R', HgX; 1,2-fc = 1,2-ferrocenediyl) that are substituted with organotin or organomercury Lewis acid moieties in ortho-position is presented. Several compounds that feature two ferrocenylphosphine-borane moieties bridged by Sn or Hg are also introduced. The products are fully characterized by multinuclear NMR spectroscopy, high-resolution MALDI-TOF mass spectrometry and elemental analysis. The attachment of the Lewis acid substituent to the same Cp ring of the ferrocene results in planar-chirality and the close proximity between the boron hydride group and the Lewis acid is expected to allow for agostic-type B-HE (E = Sn, Hg) interactions...
April 26, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28443688/use-of-c-banding-for-identifying-radiation-induced-micronuclei
#10
S C de Lima, A Amaral, T de Salazar E Fernandes
Differentiation of micronuclei (MN) caused by ionizing radiation from those caused by chemicals is a crucial step for managing treatment of individuals exposed to radiation. MN in binucleated lymphocytes in peripheral blood are widely used as biomarkers for estimating dose of radiation, but they are not specific for ionizing radiation. MN induced by ionizing radiation originate predominantly as a result of chromosome breaks (clastogenic action), whereas MN caused by chemical agents are derived from the loss of entire chromosomes (aneugenic action)...
April 26, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28443134/characterization-of-x-chromosome-gene-expression-in-bovine-blastocysts-derived-by-in-vitro-fertilization-and-somatic-cell-nuclear-transfer
#11
Byungkuk Min, Jung Sun Park, Kyuheum Jeon, Yong-Kook Kang
To better understand X-chromosome reactivation (XCR) during early development, we analyzed transcriptomic data obtained from bovine male and female blastocysts derived by in-vitro fertilization (IVF) or somatic-cell nuclear transfer (SCNT). We found that X-linked genes were upregulated by almost two-fold in female compared with male IVF blastocysts. The upregulation of X-linked genes in female IVFs indicated a transcriptional dimorphism between the sexes, because the mean autosomal gene expression levels were relatively constant, regardless of sex...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28441386/graft-derived-cell-free-dna-a-noninvasive-early-rejection-and-graft-damage-marker-in-liver-transplantation-a-prospective-observational-multicenter-cohort-study
#12
Ekkehard Schütz, Anna Fischer, Julia Beck, Markus Harden, Martina Koch, Tilo Wuensch, Martin Stockmann, Björn Nashan, Otto Kollmar, Johannes Matthaei, Philipp Kanzow, Philip D Walson, Jürgen Brockmöller, Michael Oellerich
BACKGROUND: Graft-derived cell-free DNA (GcfDNA), which is released into the blood stream by necrotic and apoptotic cells, is a promising noninvasive organ integrity biomarker. In liver transplantation (LTx), neither conventional liver function tests (LTFs) nor immunosuppressive drug monitoring are very effective for rejection monitoring. We therefore hypothesized that the quantitative measurement of donor-derived cell-free DNA (cfDNA) would have independent value for the assessment of graft integrity, including damage from acute rejection...
April 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28441264/cd19-isoforms-enabling-resistance-to-cart-19-immunotherapy-are-expressed-in-b-all-patients-at-initial-diagnosis
#13
Jeannette Fischer, Claudia Paret, Khalifa El Malki, Francesca Alt, Arthur Wingerter, Marie A Neu, Bettina Kron, Alexandra Russo, Nadine Lehmann, Lea Roth, Eva-M Fehr, Sebastian Attig, Alexander Hohberger, Thomas Kindler, Jörg Faber
B-cell acute lymphoblastic leukemia (B-ALL) is the commonest childhood cancer and the prognosis of children with relapsed or therapy refractory disease remains a challenge. Treatment with chimeric antigen receptor-modified T cells targeting the CD19 antigen (CART-19 therapy) has been presented as a promising approach toward improving the outcome of relapsed or refractory disease. However, 10%-20% of the patients suffer another relapse. Epitope-loss under therapy pressure has been suggested as a mechanism of tumor cells to escape the recognition from CART-19 therapy...
April 24, 2017: Journal of Immunotherapy
https://www.readbyqxmd.com/read/28441012/rgb-switchable-porous-electrospun-nanofiber-chemoprobe-filter-prepared-from-multifunctional-copolymers-for-versatile-sensing-of-ph-and-heavy-metals
#14
Fang-Cheng Liang, Chi Ching Kuo, Bo-Yu Chen, Chia-Jung Cho, Chih-Chien Hung, Wen-Chang Chen, Redouane Borsali
Novel red-green-blue (RGB) switchable probes based on fluorescent porous electrospun (ES) nanofibers exhibiting high sensitivity to pH and mercury ions (Hg2+) were prepared with one type of copolymer (poly(methyl methacrylatete-co-1, 8-naphthalimide derivatives-co-rhodamine derivative); poly(MMA-co-BNPTU-co-RhBAM)) by using a single-capillary spinneret. The MMA, BNPTU, and RhBAM moieties were designed to (i) permit formation of porous fibers, (ii) fluoresce for Hg2+ detection, and (iii) fluoresce for pH, respectively...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28440624/acid-base-catalysis-in-glycosidations-a-nature-derived-alternative-to-the-generally-employed-methodology
#15
Peng Peng, Richard R Schmidt
Inverting glycosyltransferases enforce in the active site an intramolecular, acid-base catalyzed glycosidation that, due to proximity of the donor anomeric carbon and the acceptor hydroxyl group, follows an SN2-type reaction. Spacers, tethering donor and acceptor via nonreacting functional groups, led in intramolecular glycosidations to excellent yields and, independent of the donor anomeric configuration, to either the α- or the β-anomer. The requirement of a demanding protecting group pattern confines the application of this efficient method...
April 25, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28439897/platelet-lysate-obtained-via-plateletpheresis-performed-in-standing-and-awake-equine-donors
#16
Scarlett M Sumner, Maria C Naskou, Merrilee Thoresen, Ian Copland, John F Peroni
BACKGROUND: Platelet preparations containing growth factors, attachment factors, and enzymes are appealing to enhance healing of injured tissues and as an alternative to xenogenic serum in cell culture media. Plateletpheresis is commonly used to collect platelets in human medicine but has not been validated in horses. STUDY DESIGN AND METHODS: Plateletpheresis to collect platelet concentrate was performed on six female, mixed breed, chemically restrained horses using commercially available apheresis equipment...
April 25, 2017: Transfusion
https://www.readbyqxmd.com/read/28438482/manufacture-and-preparation-of-human-placenta-derived-mesenchymal-stromal-cells-for-local-tissue-delivery
#17
Lee Lankford, Y Julia Chen, Zoe Saenz, Priyadarsini Kumar, Connor Long, Diana Farmer, Aijun Wang
BACKGROUND: In this study we describe the development of a Current Good Manufacturing Practice (CGMP)-compliant process to isolate, expand and bank placenta-derived mesenchymal stromal cells (PMSCs) for use as stem cell therapy. We characterize the viability, proliferation and neuroprotective secretory profile of PMSCs seeded on clinical-grade porcine small intestine submucosa extracellular matrix (SIS-ECM; Cook Biotech). METHODS: PMSCs were isolated from early gestation placenta chorionic villus tissue via explant culture...
April 21, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28437857/influence-of-inflammation-and-nitric-oxide-upon-platelet-aggregation-following-deposition-of-diesel-exhaust-particles-in-the-airways
#18
E Smyth, A Solomon, M A Birrell, M J Smallwood, P G Winyard, T D Tetley, M Emerson
BACKGROUND AND PURPOSE: Exposure to nanoparticulate pollution has been implicated in platelet-driven thrombotic events such as myocardial infarction. Inflammation and impairment of nitric oxide (NO) bioavailability have been proposed as potential causative mechanisms. It is unclear, however, whether airways exposure to combustion-derived nanoparticles such as diesel exhaust particles (DEP) or carbon black (CB) can augment platelet aggregation in vivo and the underlying mechanisms remain undefined...
April 24, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28437790/utility-in-treating-kidney-failure-in-end-stage-liver-disease-with-simultaneous-liver-kidney-transplantation
#19
Xingxing S Cheng, Margaret R Stedman, Glenn M Chertow, W Ray Kim, Jane C Tan
BACKGROUND: Simultaneous liver-kidney (SLK) transplantation plays an important role in treating kidney failure in patients with end-stage liver disease. It used 5% of deceased donor kidney transplanted in 2015. We evaluated the utility, defined as posttransplant kidney allograft lifespan, of this practice. METHODS: Using data from the Scientific Registry of Transplant Recipients, we compared outcomes for all SLK transplants between January 1, 1995, and December 3, 2014, to their donor-matched kidney used in kidney-alone (Ki) or simultaneous pancreas kidney (SPK) transplants...
May 2017: Transplantation
https://www.readbyqxmd.com/read/28437180/a-novel-drug-induced-hybrid-electrospun-pcl-cell-derived-ecm-scaffold-for-liver-tissue-engineering
#20
Rhiannon Grant, David Hay, Anthony Callanan
Liver transplant is the only treatment option for patients with end-stage liver failure, however there are too few donor livers available for transplant. Whole organ tissue engineering presents a potential solution to the problem of rapidly escalating donor liver shortages world-wide. A major challenge for liver tissue engineers is the creation of a hepatocyte micro-environment; a niche in which liver cells can survive and function optimally. While polymers and decellularized tissues pose an attractive option for scaffold manufacturing, neither alone has thus far proved sufficient...
February 24, 2017: Tissue Engineering. Part A
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