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Cytokine expression

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https://www.readbyqxmd.com/read/28817786/propofol-attenuates-bv2-microglia-inflammation-via-nmda-receptor-inhibition
#1
Qichao Wu, Yanjun Zhao, Xiangyuan Chen, Minmin Zhu, Changhong Miao
Activated microglia, involved in the occurrence and improvement of sepsis-associated encephalopathy, can induce the expression of pro-inflammatory cytokines and pro-inflammatory enzymes, resulting in inflammation-mediated neuronal cell death. It was reported that propofol could inhibit lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and pro-inflammatory enzymes expression in BV2 and primary microglial cells. However, the underlying mechanism is not well known. In the present study, we investigated whether and how propofol inhibited LPS-induced the expression of pro-inflammatory cytokines and pro-inflammatory enzymes in BV2 cells...
August 17, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28817120/the-erythropoietin-derived-peptide-mk-x-and-erythropoietin-have-neuroprotective-effects-against-ischemic-brain-damage
#2
Seung-Jun Yoo, Bongki Cho, Deokho Lee, Gowoon Son, Yeong-Bae Lee, Hyung Soo Han, Eunjoo Kim, Chanil Moon, Cheil Moon
Erythropoietin (EPO) has been well known as a hematopoietic cytokine over the past decades. However, recent reports have demonstrated that EPO plays a neuroprotective role in the central nervous system, and EPO has been considered as a therapeutic target in neurodegenerative diseases such as ischemic stroke. Despite the neuroprotective effect of EPO, clinical trials have shown its unexpected side effects, including undesirable proliferative effects such as erythropoiesis and tumor growth. Therefore, the development of EPO analogs that would confer neuroprotection without adverse effects has been attempted...
August 17, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28816577/coenzyme-q10-inhibits-th17-and-stat3-signaling-pathways-to-ameliorate-colitis-in-mice
#3
Seon-Yeong Lee, Seung Hoon Lee, Eun-Ji Yang, Jae-Kyung Kim, Eun-Kyung Kim, KyungAh Jung, Hongsoon Jung, Kyungjin Lee, Han Hee Lee, Bo-In Lee, Sung-Hwan Park, Dong Yun Shin, Mi-La Cho
Coenzyme Q10 (CoQ10) is a powerful antioxidant substance synthesized in the body. The current study aimed to determine whether CoQ10 suppresses inflammation and inhibits p-STAT3 expression in an experimental colitis mouse model. The mice were orally fed with CoQ10 once a day for 13 days. Histological analysis of the colons was performed by immunohistochemistry. Expression of IL-17, FOXP3, p53, AMPK, and mTOR and activation of p-STAT3 and p-STAT5 in lymph node and spleen tissues were detected by confocal microscopy of stained tissue sections...
August 17, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28816551/1%C3%AE-25-dihydroxyvitamin-d3-up-regulates-il-34-expression-in-sh-sy5y-neural-cells
#4
Dong Zhang, Miaomiao Li, Yang Dong, Xinhui Zhang, Xingyun Liu, Zhangming Chen, Yongji Zhu, Huiming Wang, Xuwen Liu, Jialiang Zhu, Yujun Shen, Heinrich Korner, Songcheng Ying, Shengyun Fang, Yuxian Shen
Vitamin D supplementation is regarded as a novel approach to treat Alzheimer's disease, but the underlying mechanism remains elusive. The cytokine IL-34 provides strong neuroprotective and survival signals in brain injury and neurodegeneration and could be an immunological mediator for the vitamin D-induced protection. The aim of this study was to investigate whether human IL-34 is up-regulated in neuronal cells by the hormonally active form of vitamin D, 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3]. We found that IL-34 was detectable in a variety of cell lines and its expression was strongly induced in SH-SY5Y neural cells in a dose- and time-dependent manner by 1α,25(OH)2D3 through the vitamin D receptor (VDR)...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/28816360/c-c-motif-chemokine-ligand-2-regulates-lps-induced-inflammation-and-er-stress-to-enhance-proliferation-of-bovine-endometrial-epithelial-cells
#5
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Sung-Man Kim, Gwonhwa Song
Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, C-C motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28816340/coagulation-factor-xii-regulates-inflammatory-responses-in-human-lungs
#6
Rosanna Hess, Lukasz Wujak, Christina Hesse, Katherina Sewald, Danny Jonigk, Gregor Warnecke, Hans-Gerd Fieguth, Steven de Maat, Coen Maas, Francesco Bonella, Klaus T Preissner, Benjamin Weiss, Liliana Schaefer, Wolfgang M Kuebler, Philipp Markart, Malgorzata Wygrecka
Increased procoagulant activity in the alveolar compartment and uncontrolled inflammation are hallmarks of the acute respiratory distress syndrome (ARDS). Here, we investigated whether the contact phase system of coagulation is activated and may regulate inflammatory responses in human lungs. Components of the contact phase system were characterized in bronchoalveolar lavage fluids (BALF) from 54 ARDS patients and 43 controls, and their impact on cytokine/chemokine expression in human precision cut lung slices (PCLS) was assessed by a PCR array...
August 17, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28816231/molecular-mechanisms-of-the-analgesic-action-of-wu-tou-decoction-on-neuropathic-pain-in-mice-revealed-using-microarray-and-network-analysis
#7
Yan-Qiong Zhang, Chao Wang, Qiu-Yan Guo, Chun-Yan Zhu, Chen Yan, Dan-Ni Sun, Qiong-Hong Xu, Na Lin
Wu-tou Decoction (WTD) is a classic herbal formula in traditional Chinese medicine for the treatment of joint diseases, neuropathic pain (NP) and inflammatory pain. In this study we investigated whether WTD produced analgesic action in a mouse spinal nerve ligation (SNL) model and elucidated the underlying molecular mechanisms. Mice were subjected to SNL and orally treated with WTD (3.15, 6.30 or 12.60 g·kg(-1)·d(-1)) for 21 d. SNL induced mechanical hyperalgesia and heat hyperalgesia characterized by rapid and persistent pain hypersensitivity...
August 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28815947/olive-oil-phenolics-prevent-oxysterol-induced-pro-inflammatory-cytokine-secretion-and-ros-production-in-human-pbmcs-through-modulation-of-p38-and-jnk-pathways
#8
Gessica Serra, Monica Deiana, Jeremy P E Spencer, Giulia Corona
SCOPE: The aim of the present study was to investigate the ability of extra virgin olive oil (EVOO) polyphenols to counteract the pro-inflammatory effects induced by dietary and endogenous oxysterols in ex-vivo immune cells. METHODS AND RESULTS: Peripheral blood mononuclear cells (PBMCs), separated from the whole blood of healthy donors, were utilised and were stimulated with an oxysterols mixture, in the presence of physiologically relevant concentrations of the EVOO polyphenols, hydroxytyrosol (HT), tyrosol (TYR) and homovanillic alcohol (HVA)...
August 16, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28815854/expression-of-natural-cytotoxicity-receptors-and-cytokine-production-on-endometrial-natural-killer-cells-in-women-with-recurrent-pregnancy-loss-or-implantation-failure-and-the-expression-of-natural-cytotoxicity-receptors-on-peripheral-blood-natural-killer-cells
#9
Atsushi Fukui, Ayano Funamizu, Rie Fukuhara, Hiroaki Shibahara
AIM: Natural cytotoxicity receptors (NCR) are unique markers that regulate natural killer (NK) cell cytotoxicity and cytokine production. In this study, we investigated the expression of NCR (NKp46, NKp44, and NKp30) and cytokine production in NK cells derived from the uterine endometrium of women with recurrent pregnancy loss (RPL). We also investigated the expression of NCR in peripheral blood NK cells in pregnant women with and without a history of RPL. METHODS: The expression of NCR (NKp46, NKp44, and NKp30) in NK cells (CD56(dim) and CD56(bright) ) in the uterine endometrium was analyzed using 3-color flow cytometry...
August 17, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28815719/cytokine-profiling-of-mcf-7-cell-line-2d-progressive-3d-and-3d-revert-cultures
#10
Aishwarya Subramaniyan, Maddaly Ravi
Cancer cytokines are known to mediate several complex cancer cell physiologies. Also, cancer cells themselves are known to secrete cytokines whose expressions and net inducible results are controlled by a variety of factors. We profiled a few cytokines secreted by 2D, 3D aggregates and the 3DRs of MCF-7 cell line at various time points. Several cytokines were seen more expressed by 3D cultures on the 4(th) day and IL-10 peaked on the day 1 of 3D cultures while TNF-α level peaked on the 7(th) day. α-Defensin, SDF-7 and TGF-β also showed markedly higher levels...
August 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28815701/lincrna-dyn-lrb2-2-upregulates-cholesterol-efflux-by-decreasing-tlr2-expression-in-macrophages
#11
Yongqiang Li, Shuxin Shen, Shoukun Ding, Lixia Wang
OBJECTIVE: This study is designed to determine whether lincRNA-DYNLRB2-2 could promote cholesterol efflux through regulating the expression of TLR2. METHODS: THP-1 and RAW264.7 cells were incubated with oxLDL for 48 h to induce the formation of foam cells, and ORO staining was performed and intracellular cholesterol contents were measured by HPLC assay. qRT-PCR and Western blotting were performed to detect mRNA and protein expression levels, respectively. Lentiviral vector LV-DYNLRB2-2 and lincRNA-DYNLRB2-2 siRNA was constructed to explore its potential role...
August 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28815581/th1-polarizing-%C3%AE-%C3%AE-t-cells-and-scavenger-receptors-contribute-to-the-pathogenesis-of-pemphigus-vulgaris
#12
Dayasagar Das, Vivek Anand, Sujay Khandpur, V K Sharma, Alpana Sharma
Gamma delta T cells (γδ T cells) and Scavenger receptors are key innate immune machinery playing significant role in regulating immune homeostasis at epithelial surface. The roles of these immune components are not yet characterized for the autoimmune skin disorder Pemphigus Vulgaris (PV). Phenotyping and frequency of γδ T cells estimated by Flowcytometry has shown increased frequency of γδ T cells (6.7% vs. 4.4%) producing IFN-γ (35.2% vs. 26.68%), in circulation of patients compared to controls. Dual cytokine (IFN-γ & IL-4) secreting γδ T cells indicate plasticity of these cells...
August 16, 2017: Immunology
https://www.readbyqxmd.com/read/28815218/capturing-the-systemic-immune-signature-of-a-norovirus-infection-an-n-of-1-case-study-within-a-clinical-trial
#13
Antony J Cutler, Joao Oliveira, Ricardo C Ferreira, Ben Challis, Neil M Walker, Sarah Caddy, Jia Lu, Helen E Stevens, Deborah J Smyth, Marcin L Pekalski, Jane Kennet, Kara M D Hunter, Ian Goodfellow, Linda S Wicker, John A Todd, Frank Waldron-Lynch
BACKGROUND:  The infection of a participant with norovirus during the adaptive study of interleukin-2 dose on regulatory T cells in type 1 diabetes (DILT1D) allowed a detailed insight into the cellular and cytokine immune responses to this prevalent gastrointestinal pathogen. METHODS:  Serial blood, serum and peripheral blood mononuclear cell (PBMC) samples were collected pre-, and post-development of the infection. To differentiate between the immune response to norovirus and to control for the administration of a single dose of aldesleukin (recombinant interleukin-2, rIL-2) alone, samples from five non-infected participants administered similar doses were analysed in parallel...
2017: Wellcome Open Research
https://www.readbyqxmd.com/read/28814613/osteoimmunology-the-conceptual-framework-unifying-the-immune-and-skeletal-systems
#14
REVIEW
Kazuo Okamoto, Tomoki Nakashima, Masahiro Shinohara, Takako Negishi-Koga, Noriko Komatsu, Asuka Terashima, Shinichiro Sawa, Takeshi Nitta, Hiroshi Takayanagi
The immune and skeletal systems share a variety of molecules, including cytokines, chemokines, hormones, receptors, and transcription factors. Bone cells interact with immune cells under physiological and pathological conditions. Osteoimmunology was created as a new interdisciplinary field in large part to highlight the shared molecules and reciprocal interactions between the two systems in both heath and disease. Receptor activator of NF-κB ligand (RANKL) plays an essential role not only in the development of immune organs and bones, but also in autoimmune diseases affecting bone, thus effectively comprising the molecule that links the two systems...
October 1, 2017: Physiological Reviews
https://www.readbyqxmd.com/read/28814601/il-15-complexes-induce-migration-of-resting-memory-cd8-t-cells-into-mucosal-tissues
#15
Ryan T Sowell, Josef W Goldufsky, Magdalena Rogozinska, Zurisaday Quiles, Yanxia Cao, Eliseo F Castillo, Alison Finnegan, Amanda L Marzo
IL-15 is an essential cytokine known to promote T cell survival and activate the effector function of memory phenotype CD8 T cells. Blocking IL-15 signals also significantly impacts tissue-specific effector and memory CD8 T cell formation. In this study, we demonstrate that IL-15 influences the generation of memory CD8 T cells by first promoting their accumulation into mucosal tissues and second by sustaining expression of Bcl-6 and T-bet. We show that the mechanism for this recruitment is largely dependent on mammalian target of rapamycin and its subsequent inactivation of FoxO1...
August 16, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28814530/a-high-fat-diet-decreases-bone-mass-in-growing-mice-with-systemic-chronic-inflammation-induced-by-low-dose-slow-release-lipopolysaccharide-pellets
#16
Jay J Cao, Brian R Gregoire, Chwan-Li Shen
Background: Chronic inflammation is associated with increased bone resorption and is linked to osteopenia, or low bone mass. Obesity is also associated with low-grade chronic upregulation of inflammatory cytokines.Objective: This study investigated the effect of high-fat (HF) diet-induced obesity on bone structure changes in growing mice with existing systemic chronic inflammation induced by low-dose, slow-release lipopolysaccharide (LPS).Methods: Forty-eight 6-wk-old female C57BL/6 mice were randomly assigned to 4 treatment groups (n = 12/group) in a 2 × 2 factorial design-control (placebo) or LPS treatment (1...
August 16, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28814066/mice-expressing-human-erap1-variants-associated-with-ankylosing-spondylitis-have-altered-t-cell-repertoires-and-nk-cell-functions-as-well-as-increased-in-utero-and-perinatal-mortality
#17
David P W Rastall, Fadel S Alyaquob, Patrick O'Connell, Yuliya Pepelyayeva, Douglas Peters, Sarah Godbehere-Roosa, Cristiane Pereira-Hicks, Yasser A Aldhamen, Andrea Amalfitano
Specific variants of endoplasmic reticulum-associated aminopeptidase 1 (ERAP1) identified by genome-wide association study modify the risk for developing ankylosing spondylitis. We previously confirmed that disease-associated ERAP1 variants have altered enzymatic abilities that can impact upon the production of pro-inflammatory cytokines from cells expressing the same ERAP1 variants. To determine if these ERAP1 variants also impacted immune responses in vivo, we generated two strains of transgenic mice expressing human ERAP1 genes containing non-synonymous single-nucleotide polymorphisms associated with an increased (ERAP1-High) or decreased (ERAP1-Low) risk for developing autoimmune disease...
June 1, 2017: International Immunology
https://www.readbyqxmd.com/read/28813781/bee-venom-decreases-lps-induced-inflammatory-responses-in-bovine-mammary-epithelial-cells
#18
Chang Hee Jeong, Wei Nee Cheng, Hyojin Bae, Kyung Woo Lee, Sang Mi Han, Michael C Petriello, Hong Gu Lee, Han Geuk Seo, Sung Gu Han
The world dairy industry has long been challenged by bovine mastitis, an inflammatory disease, which causes economic loss due to decreased milk production and quality. Attempts have been made to prevent or treat this disease with multiple approaches, primarily through increased abuse of antibiotics, but effective natural solutions remain elusive. Bee venom (BV) contains a variety of peptides (e.g., melittin) and that shows multiple bioactivities including prevention of inflammation. Thus, in the current study, it was hypothesized that BV can reduce inflammation in bovine mammary epithelial cells (MAC-T)...
August 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28813640/innate-immune-signaling-in-the-ventral-tegmental-area-contributes-to-drug-primed-reinstatement-of-cocaine-seeking
#19
Kyle T Brown, Sophia C Levis, Casey E O'Neill, Alexis L Northcutt, Timothy J Fabisiak, Linda R Watkins, Ryan K Bachtell
Cocaine addiction is a chronic relapsing disorder characterized by persistent perturbations to an organism's homeostatic processes that result in maladaptive drug seeking. Although considerable attention has been directed at the consequences of neuronal changes following chronic cocaine taking, few studies have examined the role of microglia, the brain's resident immune cells, following chronic cocaine administration. Toll-Like Receptor 4 (TLR4) is a molecular pattern receptor that recognizes pathogens, danger signals, and xenobiotics and induces proinflammatory signaling in the central nervous system...
August 13, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28813446/variable-stretch-reduces-the-pro-inflammatory-response-of-alveolar-epithelial-cells
#20
Ines Rentzsch, Cíntia L Santos, Robert Huhle, Jorge M C Ferreira, Thea Koch, Christian Schnabel, Edmund Koch, Paolo Pelosi, Patricia R M Rocco, Marcelo Gama de Abreu
Mechanical ventilation has the potential to increase inflammation in both healthy and injured lungs. Several animal studies have shown that variable ventilation recruits the lungs and reduces inflammation. However, it is unclear which cellular mechanisms are involved in those findings. We hypothesized that variable stretch of LPS-stimulated alveolar epithelial cells (AECs) reduces the production of pro-inflammatory cytokines compared to non-variable stretch. AECs were subjected to non-variable or variable cyclic stretch (sinusoidal pattern), with and without LPS stimulation...
2017: PloS One
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