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https://www.readbyqxmd.com/read/29344171/depdc7-inhibits-cell-proliferation-migration-and-invasion-in-hepatoma-cells
#1
Zhijun Liao, Xinrui Wang, Xiaojiang Wang, Lisheng Li, Dexin Lin
DEP Domain Containing 7 (DEPDC7) is highly and specifically expressed in normal liver tissue, belonging to the class of genes of liver-selective cell communication. Although the function of DEPDC7 remains poorly understood, its expression is decreased in liver cancer compared with normal liver tissues. It has previously been demonstrated that knockdown of DEPDC7 promotes cell growth, S phase entry and cell mobility and invasion in HepG2 cells. In the present study, it was shown that DEPDC7 expression is downregulated in four hepatoma cell lines (SMMC-7721, Huh-7, SK-Hep-1 and HepG2) and 48 hepatoma tissues, determined using western blot and immunohistochemical analysis...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29333089/lentivirus-mediated-shrna-targeting-cnn2-inhibits-hepatocarcinoma-in-vitro-and-in-vivo
#2
Xueqing Kang, Feng Wang, Xiuwan Lan, Xiaolong Li, Shunxin Zheng, Zhilue Lv, Yuan Zhuang, Yongxiang Zhao, Sufang Zhou
Objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high rate of mortality. Our previous study shows the expression of calponin 2 (CNN2) is up-regulated in hepatocellular carcinoma tissues, especially in metastatic ones. To better understand the role of CNN2 in HCC, RNA interference (RNAi) was used to explore its role in tumor growth and metastasis. Methods: Lentivirus-mediated CNN2-shRNA was transfected into SK-hep-1 cells, and the efficacy of CNN2 expression, cell migration, invasion, proliferation and cell cycles were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot (WB), Transwell assay, methyl thiazol tetrazolium assay and flow cytometry, respectively...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29324278/oleanane-type-triterpenoid-saponins-from-lysimachia-fortunei-maxim
#3
Shu-Lin Zhang, Zi-Ni Yang, Cui He, Hai-Bing Liao, Heng-Shan Wang, Zhen-Feng Chen, Dong Liang
Six previously undescribed oleanane-type triterpenoid saponins, fortunosides A-F, together with six known ones, were isolated from the aerial parts of Lysimachia fortunei Maxim. Their structures were established by spectroscopic data analyses (1D, 2D-NMR and HRESIMS) and chemical methods. All isolated triterpenoid saponins were evaluated for their cytotoxicity against four human liver cancer cell lines (SMMC-7721, Hep3B, HuH7, and SK-Hep-1). Three saponins with the aglycone protoprimulagenin A exhibited moderate cytotoxicity against all of the tested human cancer cell lines, with IC50 values ranging from 4...
January 8, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29277356/translocation-of-epidermal-growth-factor-egf-to-the-nucleus-has-distinct-kinetics-between-adipose-tissue-derived-mesenchymal-stem-cells-and-a-mesenchymal-cancer-cell-lineage
#4
Camila Cristina Fraga Faraco, Jerusa Araújo Quintão Arantes Faria, Marianna Kunrath Lima, Marcelo Coutinho de Miranda, Mariane Izabella Abreu de Melo, Andrea da Fonseca Ferreira, Michele Angela Rodrigues, Dawidson Assis Gomes
Nuclear Epidermal Growth Factor Receptor (EGFR) has been associated with worse prognosis and treatment resistance for several cancer types. After Epidermal Growth Factor (EGF) binding, the ligand-receptor complex can translocate to the nucleus where it functions in oncological processes. By three-dimensional quantification analysis of super-resolution microscopy images, we verified the translocation kinetics of fluorescent conjugated EGF to the nucleus in two mesenchymal cell types: human adipose tissue-derived stem cells (hASC) and SK-HEP-1 tumor cells...
December 19, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/29251325/cd90-promotes-cell-migration-viability-and-sphere%C3%A2-forming-ability-of-hepatocellular-carcinoma-cells
#5
Ketao Zhang, Siyao Che, Zheng Su, Shangyou Zheng, Huayao Zhang, Shanglin Yang, Wenda Li, Jianping Liu
Cluster of differentiation (CD)90 (Thy‑1) was proposed as a marker for the liver cancer stem cells that are responsible for tumorigenic activity, however its involvement in the progression of hepatocellular carcinoma (HCC) remains unknown. The aim of the present study was to determine the effect of CD90 on the biological functions of HCC and to investigate the associated circular RNA (circRNA) involved in this process. The analysis of the in vitro data demonstrated that CD90+ cells isolated from SK‑Hep‑1 cells exhibited increased viability, migration and invasive abilities compared with CD90‑ cells...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29199553/apoptotic-effect-of-taiwanofungus-salmoneus-agaricomycetes-mycelia-and-solid-state-fermented-products-on-cancer-cells
#6
Rao-Chi Chien, Yu-Chieh Lin, Jeng-Leun Mau
Because of the high incidence of and high mortality rates associated with cancer, effective therapy for cancer is currently an imperative issue. Taiwanofungus salmoneus, a medicinal mushroom indigenous to Taiwan, has been demonstrated to exhibit various physiological activities and harmless effects. The bioactive compounds and anticancer abilities of T. salmoneus mycelia and fermented products were evaluated in this study. Total phenols, flavonoids, and ergothioneine were detected in mycelia and fermented products, and the amount of ergothioneine was evaluated in buckwheat (56...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29159945/cantharidic-acid-induces-apoptosis-through-the-p38-mapk-signaling-pathway-in-human-hepatocellular-carcinoma
#7
I-Che Feng, Ming-Ju Hsieh, Pei-Ni Chen, Yi-Hsien Hsieh, Hsin-Yu Ho, Shun-Fa Yang, Chao-Bin Yeh
Cantharidin analogs exhibit anticancer activities, including apoptosis. However, the molecular mechanisms underlying the effects of cantharidic acid (CA), a cantharidin analog, on apoptosis in hepatocellular carcinoma (HCC) cells are unclear. Thus, in this study, we evaluated the anticancer activities of CA by investigating its ability to trigger apoptosis in SK-Hep-1 cells. Our data demonstrated that CA effectively inhibited the proliferation of SK-Hep-1 cells in a dose-dependent manner. Furthermore, CA effectively triggered cell cycle arrest and induced apoptosis, as determined by flow cytometric analysis...
November 21, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29152126/extracellular-atp-as-an-energy-and-phosphorylating-molecule-induces-different-types-of-drug-resistances-in-cancer-cells-through-atp-internalization-and-intracellular-atp-level-increase
#8
Xuan Wang, Yunsheng Li, Yanrong Qian, Yanyang Cao, Pratik Shriwas, Haiyun Zhang, Xiaozhuo Chen
Cancer cells are able to uptake extracellular ATP (eATP) via macropinocytosis to elevate intracellular ATP (iATP) levels, enhancing their survival in drug treatment. However, the involved drug resistance mechanisms are unknown. Here we investigated the roles of eATP as either an energy or a phosphorylating molecule in general drug resistance mediated by ATP internalization and iATP elevation. We report that eATP increased iATP levels and promoted drug resistance to various tyrosine kinase inhibitors (TKIs) and chemo-drugs in human cancer cell lines of five cancer types...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29128513/dihydroartemisinin-induced-caspase-dependent-apoptosis-through-inhibiting-the-specificity-protein-1-pathway-in-hepatocellular-carcinoma-sk-hep-1-cells
#9
Eunji Im, Changhwan Yeo, Hyo-Jeong Lee, Eun-Ok Lee
AIMS: Dihydroartemisinin (DHA) is a semi-synthetic derivative of artemisinin, well known for a safe and effective first-line antimalarial agent. This study investigated whether and how DHA induces apoptosis focusing on the specificity protein 1 (Sp1) pathway in hepatocellular carcinoma (HCC) SK-Hep-1 cells. MAIN METHODS: The cell viability was evaluated by MTT assay. Cell cycle analysis was performed after PI staining by flow cytometry system. Apoptosis was confirmed by DAPI staining and by detecting cytoplasmic histone-associated-DNA-fragments using a cell death detection ELISA(PLUS) kit...
November 8, 2017: Life Sciences
https://www.readbyqxmd.com/read/29098406/molecular-characterization-and-bioactivity-evaluation-of-two-novel-bombinin-peptides-from-the-skin-secretion-of-oriental-fire-bellied-toad-bombina-orientalis
#10
Xin Peng, Chang Zhou, Xuan Hou, Yao Liu, Zhengming Wang, Xiaolin Peng, Zhe Zhang, Ran Wang, Dexin Kong
Following the exploration of biochemicals in amphibian defensive skin secretion, great attention has been focused on the novel bioactive peptides with unique molecular structures and complicated features and functions. In this study, the skin secretion of Oriental fire-bellied toad, Bombina orientalis, was acquired to search peptides with therapeutic potential. Using "shotgun" cloning technique, a full-length peptide precursor co-encoding two novel bombinin peptides was cloned from the skin secretion-derived cDNA library of B...
November 2, 2017: Amino Acids
https://www.readbyqxmd.com/read/29097358/lncrna-miat-promoted-proliferation-and-invasion-of-hcc-cells-via-sponging-mir-214
#11
Xinli Huang, Yun Gao, Jianjie Qin, Sen Lu
BACKGROUND: The aberrant expression of lncRNAs has been involved in various human tumors including hepatocellular carcinoma (HCC). Our study aimed to investigate the potential molecular mechanism of lncRNA-MIAT in HCC. METHODS: The expression of MIAT and miR-214 in HCC tissues and cells was examined by qRT-PCR, and the levels of EZH2 and β-catenin were detected by western blot assay. The immunoprecipitation analysis was used to detect the level of H3/H4 histone acetylation...
November 2, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29075151/sporadic-pcdh18-somatic-mutations-in-epcam-positive-hepatocellular-carcinoma
#12
Takehiro Hayashi, Taro Yamashita, Hikari Okada, Kouki Nio, Yasumasa Hara, Yoshimoto Nomura, Tomoyuki Hayashi, Yoshiro Asahina, Mariko Yoshida, Naoki Oishi, Hajime Sunagozaka, Hajime Takatori, Masao Honda, Shuichi Kaneko
BACKGROUND: The relationship between specific genome alterations and hepatocellular carcinoma (HCC) cancer stem cells (CSCs) remains unclear. In this study, we evaluated the relationship between somatic mutations and epithelial cell adhesion molecule positive (EpCAM(+)) CSCs. METHODS: Two patient-derived HCC samples (HCC1 and HCC2) were sorted by EpCAM expression and analyzed by whole exome sequence. We measured PCDH18 expression level in eight HCC cell lines as well as HCC1 and HCC2 by real-time quantitative RT-PCR...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/29069721/evolutionary-cancer-favoring-engineered-vaccinia-virus-for-metastatic-hepatocellular-carcinoma
#13
So Young Yoo, Su-Nam Jeong, Dae Hwan Kang, Jeong Heo
Engineered vaccinia virus-based therapy shows promising results in patients with advanced hepatocellular carcinoma, although a strategic virus design for the metastatic liver and the study of its efficacy in treating the cancer has not been well assessed. In this paper, we proposed a simple and strategic virus design for targeting metastatic hepatocellular carcinoma. We developed an evolutionary cancer-favoring engineered vaccinia virus (CVV, which is produced by repeated selective replication in cancerous tissues and then deleting viral thymidine kinase genes) and investigated its therapeutic effects on metastatic liver cancer...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28943923/specific-expression-of-proton-coupled-oligopeptide-transporter-1-in-primary-hepatocarcinoma-a-novel-strategy-for-tumor-targeted-therapy
#14
Yanxia Gong, Jie Zhang, Xiang Wu, Tao Wang, Jia Zhao, Zhi Yao, Qingyu Zhang, Xi Liu, Xu Jian
Proton-coupled oligopeptide transporter 1 (PEPT1) is a membrane protein which expressed predominantly in intestine and recognized as the target of dietary nutrients (di/tripeptide) or peptidomimetic drug for delivery. The information on the existence of PEPT1 in carcinomas were limited. Our study aimed to investigate the expression profile and transport activity of PEPT1 both in human hepatocarcinoma tissues and cell lines. Western blotting and an immunofluorescence assay revealed the high level of PEPT1 protein expression in hepatocarcinoma Bel-7402, SMMC-7721, HepG2, HEP3B, SK-HEP-1 cell lines...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28928894/mzf-1-elk-1-pkc%C3%AE-is-associated-with-poor-prognosis-in-patients-with-hepatocellular-carcinoma
#15
Je-Chiuan Ye, Li-Sung Hsu, Jen-Hsiang Tsai, Hsin-Ling Yang, Meen-Woon Hsiao, Jin-Ming Hwang, Chia-Jen Lee, Jer-Yuh Liu
Background: Protein kinase C alpha (PKCα) is a key signaling molecule in human cancer development. As a therapeutic strategy, targeting PKCα is difficult because the molecule is ubiquitously expressed in non-malignant cells. PKCα is regulated by the cooperative interaction of the transcription factors myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) in human cancer cells. Methods: By conducting tissue array analysis, herein, we determined the protein expression of MZF-1/Elk-1/PKCα in various cancers...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28902357/expression-of-wnt3a-in-hepatocellular-carcinoma-and-its-effects-on-cell-cycle-and-metastasis
#16
Caijie Lu, Yifeng He, Juan Duan, Yongguang Yang, Chunqiang Zhong, Jian Zhang, Weiguo Liao, Xiaojie Huang, Runzhi Zhu, Mingyi Li
Invasion and metastasis are the primary causes of mortality from hepatocellular carcinoma (HCC). Effective inhibition against participants in the tumourigenesis and metastasis process is critical for treatment of HCC. Wnt3a is involved in the development and metastasis of many malignant tumours. However, the specific mechanisms of Wnt3a-mediated cell proliferation, invasion and metastasis in HCC remain unclear. In this study, we found that Wnt3a and its target gene c‑Myc showed higher expression in tumour tissues than normal liver tissues in HCC patients; 71...
October 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28829830/nanofibrillar-cellulose-alginate-hydrogel-coated-surgical-sutures-as-cell-carrier-systems
#17
Patrick Laurén, Petter Somersalo, Irina Pitkänen, Yan-Ru Lou, Arto Urtti, Jouni Partanen, Jukka Seppälä, Mari Madetoja, Timo Laaksonen, Antti Mäkitie, Marjo Yliperttula
Hydrogel nanomaterials, especially those that are of non-human and non-animal origins, have great potential in biomedical and pharmaceutical sciences due to their versatility and inherent soft-tissue like properties. With the ability to simulate native tissue function, hydrogels are potentially well suited for cellular therapy applications. In this study, we have fabricated nanofibrillar cellulose-alginate (NFCA) suture coatings as biomedical devices to help overcome some of the limitations related to cellular therapy, such as low cell survivability and distribution out of target tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28800461/design-synthesis-and-biological-evaluation-of-novel-3-substituted-imidazo-1-2-a-pyridine-and-quinazolin-4-3h-one-derivatives-as-pi3k%C3%AE-inhibitors
#18
Yan-Hua Fan, Wei Li, Dan-Dan Liu, Meng-Xuan Bai, Hong-Rui Song, Yong-Nan Xu, SangKook Lee, Zhi-Peng Zhou, Jian Wang, Huai-Wei Ding
Phosphatidylinositol 3-kinase (PI3K) is a pivotal regulator of intracellular signaling pathways and considered as a promising target in the development of a therapeutic treatment of cancer. Among the different PI3K subtypes, the PIK3CA gene encoding PI3K p110α is frequently mutated and overexpressed in majority of human cancers. Therefore, the inhibition of PI3Kα has been considered to be an efficient approach for the treatment of cancer. In this study, two series compounds containing hydrophilic group in imidazo[1,2-a]pyridine and quinazolin-4(3H)-one were synthesized and their antiproliferative activities against five cancer cell lines, including HCT-116, SK-HEP-1, MDA-MB-231, SNU638 and A549, were evaluated...
October 20, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28723216/lycopene-inhibits-metastasis-of-human-liver-adenocarcinoma-sk-hep-1-cells-by-downregulation-of-nadph-oxidase-4-protein-expression
#19
Bo-Yi Jhou, Tuzz-Ying Song, Inn Lee, Miao-Lin Hu, Nae-Cherng Yang
NADPH oxidase 4 (NOX4), with the sole function to produce reactive oxygen species (ROS), can be a molecular target for disrupting cancer metastasis. Several studies have indicated that lycopene exhibited anti-metastatic actions in vitro and in vivo. However, the role of NOX4 in the anti-metastatic action of lycopene remains unknown. Herein, we first confirmed the anti-metastatic effect of lycopene (0.1-5 μM) on human liver adenocarcinoma SK-Hep-1 cells. We showed that lycopene significantly inhibited NOX4 protein expression, with the strongest inhibition of 64...
August 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28722351/%C3%AE-mangostin-suppresses-human-hepatocellular-carcinoma-cell-invasion-through-inhibition-of-mmp-2-and-mmp-9-expression-and-activating-the-erk-and-jnk-pathways
#20
Chien-Feng Huang, Ying-Hock Teng, Fung-Jou Lu, Wen-Hung Hsu, Chia-Liang Lin, Chia-Chen Hung, Jai-Nien Tung, Yi-Hsien Hsieh, Chung-Jung Liu
β-mangostin is a dietary xanthone that has been reported to have the anticancer properties in some human cancer cell types. However, the antimetastatic effect and molecular mechanism of β-mangostin action in human hepatocellular carcinoma (HCC) cells remain unknown. In this study, we found that β-mangostin did not induce cytotoxicity in human HCC cells (SK-Hep-1, Huh-7 and HA22T/VGH cells). β-mangostin could inhibit migration and invasion of human HCC cells. Meanwhile, β-mangostin significantly decreased the protein activities and expression of matrix metalloproteinase (MMP)-2 and MMP-9 via increasing the activation of MEK1/2, ERK1/2, MEK4 and JNK1/2 signaling pathways...
July 19, 2017: Environmental Toxicology
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