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https://www.readbyqxmd.com/read/28442387/establishment-of-microrna-delivery-system-by-pp7-bacteriophage-like-particles-carrying-cell-penetrating-peptide
#1
Yanli Sun, Yanhua Sun, Ronglan Zhao
MicroRNAs have great therapeutic potential in cancer and other diseases. However, their instability and low in vivo delivery efficiency limits their application. Recombinant PP7 bacteriophage-based virus-like particles (VLPs) could protect microRNAs against rapid degradation by RNase by packaging specific exogenous pre-microRNAs using the pac site. Insertion of a cell-penetrating peptide (CPP) into the AB-loop of VLPs could significantly improve the delivery efficiency of microRNAs into mammalian cells. Unlike other microRNA delivery methods (viral or non-viral vectors), recombinant PP7 VLPs carrying a CPP and microRNA could be efficiently expressed in Escherichia coli using the one-plasmid double expression system...
April 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28442344/mir-221-regulates-cd44-in-hepatocellular-carcinoma-through-the-pi3k-akt-mtor-pathway
#2
Jihye Kim, Jinmai Jiang, Mohamed Badawi, Thomas D Schmittgen
CD44 and miR-221 are upregulated in hepatocellular carcinoma (HCC) cell lines and tumors, however a connection between the two has not been identified. As the expression of miR-221 directly correlated with CD44 in HCC cells, we hypothesized that miR-221 may directly or indirectly regulate CD44 expression. Inhibition of miR-221 with antisense in Sk-Hep-1 or SNU-449 cell lines reduced CD44 protein expression while miR-221 mimic increased CD44 protein levels. miR-221 antisense did not alter the CD44 mRNA levels in Sk-Hep-1 or SNU-449 cells suggesting that regulation of CD44 protein occurs post transcriptionally...
April 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28375585/down-regulation-of-talin1-promotes-hepatocellular-carcinoma-progression-via-activation-of-the-erk1-2-pathway
#3
Chen Peijuan, Lei Ling, Wang Jian, Zou Xuejing, Zhang Dongyan, Deng Ling, Wu Dehua
Talin1 is an adaptor protein that conjugates integrins to the cytoskeleton and regulates integrins and focal adhesion signaling. Several studies have found that Talin1 is over-expressed in several tumor types and promotes tumor progression. However, the explicit role of Talin1 in hepatocellular carcinoma (HCC) progression is still unclear and its functional mechanism remains largely unknown. In this study, we showed a trend of gradually decreasing expression of Talin1 from normal liver tissues to hepatocirrhosis, liver hyperplasia, the corresponding adjacent non-tumor, primary HCC and eventually metastatic foci, indicating that Talin1 maybe correlate with HCC initiation to progression...
April 4, 2017: Cancer Science
https://www.readbyqxmd.com/read/28341836/fam46c-is-critical-for-the-anti-proliferation-and-pro-apoptotic-effects-of-norcantharidin-in-hepatocellular-carcinoma-cells
#4
Qiao-Yan Zhang, Xiao-Qiang Yue, Yi-Ping Jiang, Ting Han, Hai-Liang Xin
Norcantharidin (NCTD), a demethylated analog of cantharidin derived from Chinese traditional medicine blister beetle, has been currently used as an anticancer drug for various cancers including hepatocellular carcinoma (HCC). In this study, for a more comprehensive understanding of the targets of NCTD in HCC, next-generation RNA-Seq was utilized. We revealed that the expression of FAM46C, which has been reported as a tumor suppressor for multiple myeloma, was enhanced after NCTD treatment. Re-analysis of TCGA (The Cancer Genome Atlas) LIHC (liver hepatocellular carcinoma) dataset demonstrated that FAM46C expression was significantly lower in HCC tissues than in normal liver tissues...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28333112/synthesis-and-cytotoxicity-of-n-substituted-dibenzo-a-j-xanthene-3-11-dicarboxamide-derivatives
#5
Yongbin Song, Yihui Yang, Lijun Wu, Naiwei Dong, Shang Gao, Hongrui Ji, Xia Du, Bo Liu, Guoyou Chen
In order to study the structure-activity relationships of xanthene derivatives, four series of N-substituted 14-aryl-14H-dibenzo[a,j]xanthene-3,11-dicarboxamide derivatives were synthesized. The structures of all compounds were identified by ¹H-NMR, HR-MS and IR spectra, in which compounds 6a-h were further identified by (13)C-NMR spectra. The in vitro antitumor activity of the synthesized compounds was tested by MTT assay. Most of them displayed strong inhibitory activity on human hepatocellular carcinoma cell lines (SK-HEP-1, HepG2 and SMMC-7721 cells) and acute promyelocytic leukemia NB4 cells...
March 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28276313/inhibition-of-mmp-2-expression-enhances-the-anti-tumor-effect-of-sorafenib-in-hepatocellular-carcinoma-by-suppressing-pi3k-akt-mtor-pathway
#6
Wenliang Tan, Sicong Zhu, Jun Cao, Lei Zhang, Wenda Li, Kairui Liu, Jinyi Zhong, Changzhen Shang, Yajin Chen
Sorafenib has been globally approved as the standard treatment for patients with advanced Hepatocellular carcinoma (HCC). However, the response rate of HCC patients to sorafenib is limited due to tumor recurrence and metastasis. Therefore, seeking combined therapeutic strategies with sorafenib is necessary to improve the anti-tumor efficiency. Here we demonstrated that expression of MMP-2 positively correlated with the migration ability of HCC cells. Cells with higher MMP-2 expression (SK-HEP-1 cells) were less sensitive to sorafenib than those with lower MMP-2 expression (HepG2 cells)...
March 8, 2017: Oncology Research
https://www.readbyqxmd.com/read/28199981/growth-differentiation-factor-15-induces-growth-and-metastasis-of-human-liver-cancer-stem-like-cells-via-akt-gsk-3%C3%AE-%C3%AE-catenin-signaling
#7
Qiong Xu, Hai-Xu Xu, Jin-Ping Li, Song Wang, Zheng Fu, Jing Jia, Li Wang, Zhi-Feng Zhu, Rong Lu, Zhi Yao
Cancer stem cells in liver cancer are thought to be responsible for tumor recurrence and metastasis. However, the factors that mediate this mechanism have yet to be completely elucidated. In this study, we isolated CD13+CD44+ sphere cells (SCs) derived from liver cancer tissues and SK-Hep-1 cells, which possessed cancer stem cell-like properties. Through cytokine array analysis, growth differentiation factor 15 (GDF15) was significantly increased in SCs. Clinical data showed GDF15 was overexpressed in liver cancer tissues and was positively related to pathological grading...
March 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28191864/overexpression-of-nad-p-h-quinone-oxidoreductase-1-inhibits-hepatocellular-carcinoma-cell-proliferation-and-induced-apoptosis-by-activating-ampk-pgc-1%C3%AE-pathway
#8
Xin Zhang, Kun Han, Dong-Hong Yuan, Cun-Ying Meng
Hepatocellular carcinoma (HCC) is the most common lethal malignancy and a leading cause of malignancy-associated death in many countries, but mainly in Asia. Expression of the NAD(P)H:quinone oxidoreductase 1 (NQO1) protein is involved in the growth of various human cancers, including HCC. NQO1 is considered an inhibitor of cancers. The present study aimed to investigate the function and mechanism of NQO1 in HCC. In this study, we found that NQO1 overexpression decreased HCC cell SK-hep-1 and Hep3B cell proliferation and induced apoptosis...
April 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28103717/baccharis-trimera-inhibits-reactive-oxygen-species-production-through-pkc-and-down-regulation-p47-phox-phosphorylation-of-nadph-oxidase-in-sk-hep-1-cells
#9
Glaucy Rodrigues de Araújo, Ana Carolina Silveira Rabelo, Janaína Serenato Meira, Joamyr Victor Rossoni-Júnior, William de Castro-Borges, Renata Guerra-Sá, Maurício Azevedo Batista, Denise da Silveira-Lemos, Gustavo Henrique Bianco de Souza, Geraldo Célio Brandão, Míriam Martins Chaves, Daniela Caldeira Costa
Baccharis trimera, popularly known as "carqueja", is a native South-American plant possessing a high concentration of polyphenolic compounds and therefore high antioxidant potential. Despite the antioxidant potential described for B. trimera, there are no reports concerning the signaling pathways involved in this process. So, the aim of the present study was to assess the influence of B. trimera on the modulation of PKC signaling pathway and to characterize the effect of the nicotinamide adenine dinucleotide phosphate oxidase enzyme (NOX) on the generation of reactive oxygen species in SK Hep-1 cells...
February 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28008721/therapy-of-primary-and-metastatic-liver-cancer-by-human-ips-cell-derived-myeloid-cells-producing-interferon-%C3%AE
#10
Masataka Sakisaka, Miwa Haruta, Yoshihiro Komohara, Satoshi Umemoto, Keiko Matsumura, Tokunori Ikeda, Motohiro Takeya, Yukihiro Inomata, Yasuharu Nishimura, Satoru Senju
BACKGROUND: iPS-ML are myeloid lineage cells with a proliferative capacity derived from induced pluripotent stem (iPS) cells. This study aimed to examine therapeutic effect of iPS-ML producing interferon-β (iPS-ML/IFN-β) towards primary and metastatic liver cancer and investigate the mechanism of that effect. METHODS: We established a xenograft model of liver metastasis by injecting the spleen of SCID mice with MKN-45 human gastric cancer cells and also a primary liver cancer model by injecting SK-HEP-1 human hepatocellular carcinoma cells into the liver...
February 2017: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/28000898/regorafenib-induces-extrinsic-and-intrinsic-apoptosis-through-inhibition-of-erk-nf-%C3%AE%C2%BAb-activation-in-hepatocellular-carcinoma-cells
#11
Jai-Jen Tsai, Po-Jung Pan, Fei-Ting Hsu
The aim of the present study was to investigate the role of NF-κB inactivation in regorafenib-induced apoptosis in human hepatocellular carcinoma SK-HEP-1 cells. SK-HEP-1 cells were treated with different concentrations of the NF-κB inhibitor 4-N-[2-(4-phenoxyphenyl)ethyl]quinazoline-4,6-diamine (QNZ) or regorafenib for different periods. The effects of QNZ and regorafenib on cell viability, expression of NF-κB-modulated anti-apoptotic proteins and apoptotic pathways were analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blotting, DNA gel electrophoresis, flow cytometry and NF-κB reporter gene assay...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/27989599/lycopene-inhibits-reactive-oxygen-species-production-in-sk-hep-1%C3%A2-cells-and-attenuates-acetaminophen-induced-liver-injury-in-c57bl-6-mice
#12
Ana Carla Balthar Bandeira, Talita Prato da Silva, Glaucy Rodrigues de Araujo, Carolina Morais Araujo, Rafaella Cecília da Silva, Wanderson Geraldo Lima, Frank Silva Bezerra, Daniela Caldeira Costa
Our aim was to investigate the antioxidant potential of lycopene in different experimental liver models: in vitro, to evaluate the influence of lycopene on reactive oxygen species (ROS) production mediated by the PKC pathway and in vivo, to evaluate the protective effects of lycopene in an experimental model of hepatotoxicity. The in vitro study assessed the lycopene antioxidant potential by the quantification of ROS production in SK-Hep-1 cells unstimulated or stimulated by an activator of the PKC pathway...
February 1, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27941649/extract-of-monascus-purpureus-cwt715-fermented-from-sorghum-liquor-biowaste-inhibits-migration-and-invasion-of-sk-hep-1-human-hepatocarcinoma-cells
#13
Wen-Teish Chang, Cheng-Hung Chuang, Wan-Ju Lee, Chin-Shiu Huang
Liver cancer is the most endemic cancer in a large region of the world. This study investigated the anti-metastatic effects of an extract of Monascus purpureus CWT715 (MP) fermented from sorghum liquor biowaste and its mechanisms of action in highly metastatic human hepatocarcinoma SK-Hep-1 cells. Kinmen sorghum liquor waste was used as the primary nutrient source to produce metabolites (including pigments) of MP. In the presence of 10 µg/mL MP-fermented broth (MFB), the anti-invasive activity increased with increasing fermentation time reaching a maximum at six days of fermentation...
December 8, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27914985/combination-therapy-with-doxorubicin-loaded-galactosylated-poly-ethyleneglycol-lithocholic-acid-to-suppress-the-tumor-growth-in-an-orthotopic-mouse-model-of-liver-cancer
#14
Bijay Singh, Yoonjeong Jang, Sushila Maharjan, Hyeon-Jeong Kim, Ah Young Lee, Sanghwa Kim, Nomundelger Gankhuyag, Myeon-Sik Yang, Yun-Jaie Choi, Myung-Haing Cho, Chong-Su Cho
Despite advances in technology, neither conventional anti-cancer drugs nor current nanoparticle (NP) drugs have gained substantial success in cancer treatment. While conventional chemotherapy drugs have several limitations such as low potency, poor in vivo stability and limited bioavailability, non-specific targeting of NP drugs diminishes their potency at actual target sites. In addition, the development of drug resistance to anti-cancer drugs is another challenging problem. To overcome these limitations, we aimed to develop a polymer-drug conjugate, which functions as an active NP drug and drug carrier both, to deliver a chemotherapeutic drug for combination therapy...
February 2017: Biomaterials
https://www.readbyqxmd.com/read/27895800/a-novel-curcumin-analogue-is-a-potent-chemotherapy-candidate-for-human-hepatocellular-carcinoma
#15
Ji-An Zhao, Mei-Xiang Sang, Cui-Zhi Geng, Shi-Jie Wang, Bao-En Shan
Curcumin (CUR) has been demonstrated to protect against carcinogenesis and to prevent tumor development in cancer; however, the clinical application of CUR is limited by its instability and poor metabolic properties. The present study offers an strategy for a novel CUR analogue, (1E,4E)-1,5-bis(2-bromophenyl)penta-1,4-dien-3-one (GL63), to be used as a potential therapeutic agent for hepatocellular carcinoma (HCC) in vitro and in vivo. The current study demonstrated that GL63 exhibited more potent inhibition of proliferation of HCC cells than CUR...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27785478/outcome-of-intensive-outpatient-rehabilitation-and-bracing-in-an-adult-patient-with-scheuermann-s-disease-evaluated-by-radiologic-imaging-a-case-report
#16
Hagit Berdishevsky
BACKGROUND: No studies examine the efficacy of intensive specific physical therapy (PT) exercises along with brace for the adult with Scheuermann's kyphosis (SK). The aim of this study was to examine the effects of intensive PT based on the Barcelona Scoliosis Physical Therapy School (BSPTS) and SpinoMed brace on a 76-year-old female with SK. CASE DESCRIPTION: A 76-year-old female, diagnosed with SK as an adolescent, presented in October 2014 with thoracic hyperkyphosis T1 to T12 Cobb angle of 85° and lumbar hyper lordosis L1 to L5 Cobb angle of 70°...
2016: Scoliosis and Spinal Disorders
https://www.readbyqxmd.com/read/27770311/up-regulation-of-small-nucleolar-rna-78-is-correlated-with-aggressive-phenotype-and-poor-prognosis-of-hepatocellular-carcinoma
#17
Pei Ma, Haitao Wang, Lu Han, Wei Jing, Xin Zhou, Zhisu Liu
Small nucleolar RNAs (snoRNAs) as a novel molecular species may have significant and comprehensive influences on the development and progression of hepatocellular carcinoma (HCC). We recently characterized snoRNA transcriptome signatures in HCC tissues by small RNA sequencing and found that small nucleolar RNA 78 (SNORD78) was associated with HCC. However, little is known about the pathological role of SNORD78 in HCC patients. This study aimed to profile SNORD78 expression signature and then to explore the pathogenesis of SNORD78 in HCC...
October 21, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27765853/ascochlorin-enhances-the-sensitivity-of-doxorubicin-leading-to-the-reversal-of-epithelial-to-mesenchymal-transition-in-hepatocellular-carcinoma
#18
Xiaoyun Dai, Kwang Seok Ahn, Ling Zhi Wang, Chulwon Kim, Amudha Deivasigamni, Frank Arfuso, Jae-Young Um, Alan Prem Kumar, Young-Chae Chang, Dhiraj Kumar, Gopal C Kundu, Junji Magae, Boon Cher Goh, Kam Man Hui, Gautam Sethi
Increasing evidence has indicated that epithelial-to-mesenchymal transition (EMT) at the advanced stage of liver cancer not only has the ability to self-renew and progress cancer, but also enables greater resistance to conventional chemo- and radiotherapies. Here, we report that ascochlorin (ASC), an isoprenoid antibiotic, could potentiate the cytotoxic effect of doxorubicin on HCCLM3, SNU387, SNU49, and SK-Hep-1 hepatocellular carcinoma cells, which had a predominantly mesenchymal signature with low expression of E-cadherin but high expression of N-cadherin...
December 2016: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/27765630/long-non-coding-rna-linc-cdh4-2-inhibits-the-migration-and-invasion-of-hcc-cells-by-targeting-r-cadherin-pathway
#19
Yunzhen Gao, Gaoxiong Wang, Cuilin Zhang, Minjie Lin, Xiaolong Liu, Yongyi Zeng, Jingfeng Liu
Long non-coding RNAs (LncRNAs) have played very important roles in the malignancy behaviors of hepatocellular carcinoma (HCC). Linc-cdh4-2 (TCONS_00027978) is a novel LncRNA that has been identified in HCC tissues from our previous study. Overexpression of linc-cdh4-2 in HCC cell lines (SK-Hep-1 and Huh7) significantly decreases the migration and invasion abilities of these cells, while knockdown the expression of linc-cdh4-2 significantly increases the migration and invasion abilities. Interestingly, neither the over expression nor the knock down of linc-cdh4-2 could affect the viability and proliferation of HCC cells...
October 17, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27705003/steroid-glycosides-from-the-starfish-pentaceraster-gracilis
#20
Le Thi Vien, Bui Thi Ngoan, Tran Thi Hong Hanh, Le Ba Vinh, Do Cong Thung, Do Thi Thao, Nguyen Van Thanh, Nguyen Xuan Cuong, Nguyen Hoai Nam, Phan Van Kiem, Chau Van Minh
Using combined chromatographic separations, two new steroid glycosides namely pentacerosides A (1) and B (2), and four known compounds were isolated from the methanol extract of the starfish Pentaceraster gracilis. Their structures were determined on the basis of spectroscopic data ((1)H and (13)C NMR, HSQC, HMBC, (1)H-(1)H COSY, ROESY, and FT-ICR-MS) and by comparing obtained results to the literature values. Among the isolated compounds, only maculatoside (5) showed significant cytotoxic effect against Hep-G2 (IC50 = 16...
May 2017: Journal of Asian Natural Products Research
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