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Fetal healing

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https://www.readbyqxmd.com/read/27903619/interleukin-10-mediated-regenerative-postnatal-tissue-repair-is-dependent-on-regulation-of-hyaluronan-metabolism-via-fibroblast-specific-stat3-signaling
#1
Swathi Balaji, Xinyi Wang, Alice King, Louis D Le, Sukanta S Bhattacharya, Chad M Moles, Manish J Butte, Vinicio A de Jesus Perez, Kenneth W Liechty, Thomas N Wight, Timothy M Crombleholme, Paul L Bollyky, Sundeep G Keswani
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that regulates hyaluronan (HA) metabolism and drives adult fibroblasts to synthesize an HA-rich pericellular matrix, which mimics the fetal regenerative wound healing phenotype with reduced fibrosis. By using cre-lox-mediated novel, inducible, fibroblast-, keratinocyte-, and wound-specific STAT3 knockdown postnatal mice-plus syngeneic fibroblast cell-transplant models-we demonstrate that the regenerative effects of IL-10 in postnatal wounds are dependent on HA synthesis and fibroblast-specific STAT3-dependent signaling...
November 30, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27882464/analysis-of-reparative-activity-of-platelet-lysate-effect-on-cell-monolayer-recovery-in-vitro-and-skin-wound-healing-in-vivo
#2
N S Sergeeva, Ya D Shanskii, I K Sviridova, P A Karalkin, V A Kirsanova, S A Akhmedova, A D Kaprin
Platelet lysate prepared from donor platelet concentrate and pooled according to a developed technique stimulates migration of multipotent mesenchymal stromal cells of the human adipose tissue and promotes healing of the monolayer defect in cultures of human fibroblasts and multipotent mesenchymal stromal cells in vitro in concentrations close those of fetal calf serum (5-10%). Lysate of platelets from platelet-rich rat blood plasma stimulated healing of the skin defect by promoting epithelialization and granulation tissue formation...
November 23, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27788827/in%C3%A2-vitro-effects-of-thymoglobulin-in-human-embryonic-kidney-cell-line-hek293-in-culture
#3
F Jurenec, K Mihovilović, K Špiranec, A Sinđić, M Knotek
BACKGROUND: Anti-thymocyte globulins are polyclonal T-cell-depleting immunoglobulins used in induction of immunosuppression in kidney transplant recipients. Thymoglobulin is purified rabbit immunoglobulin (Ig)G, obtained by immunization of rabbits with fetal human thymus, which depletes T lymphocytes by complement-dependent lysis and apoptosis, reduces production of cytokines, and decreases expression of adhesion molecules in endothelial cells. METHODS: To determine possible direct effects of Thymoglobulin on kidney cells during transplantation, we used the Human Embryonic Kidney cell line (HEK293) in culture...
October 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27774106/highly-efficient-in-vitro-reparative-behaviour-of-dental-pulp-stem-cells-cultured-with-standardised-platelet-lysate-supplementation
#4
Pasquale Marrazzo, Francesco Paduano, Francesca Palmieri, Massimo Marrelli, Marco Tatullo
Dental pulp is an accessible source of multipotent mesenchymal stromal cells (MSCs). The perspective role of dental pulp stem cells (DPSCs) in regenerative medicine demands an in vitro expansion and in vivo delivery which must deal with the safety issues about animal serum, usually required in cell culture practice. Human platelet lysate (PL) contains autologous growth factors and has been considered as valuable alternative to fetal bovine serum (FBS) in cell cultures. The optimum concentration to be added of such supplement is highly dependent on its preparation whose variability limits comparability of results...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27773739/basic-fibroblast-growth-factor-induces-angiogenic-properties-of-fibrocytes-to-stimulate-vascular-formation-during-wound-healing
#5
Miho Nakamichi, Yuri Akishima-Fukasawa, Chie Fujisawa, Tetsuo Mikami, Kiyoshi Onishi, Yoshikiyo Akasaka
The precise role of fibrocytes in angiogenesis in wounds remains unclear. We therefore demonstrated the specific changes in fibrocyte accumulation for angiogesis in basic fibroblast growth factor (bFGF)-treated wounds. bFGF-treated wounds exhibited marked formation of arterioles and inhibition of podoplanin(+) lymph vessels that were lacking in vascular endothelial growth factor-A-treated wounds. Real-time PCR in bFGF-treated wounds manifested enhanced expression of CD34, CD31, and bFGF mRNA and reduced expression of podoplanin and collagen type I, III, and IV mRNA...
October 20, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27769742/all-trans-retinoic-acid-promotes-wound-healing-of-primary-amniocytes-through-the-induction-of-loxl4-a-member-of-the-lysyl-oxidase-family
#6
Marion Rouzaire, Aurélie Comptour, Corinne Belville, Damien Bouvier, Gaël Clairefond, Flora Ponelle, Vincent Sapin, Denis Gallot, Loïc Blanchon
Thirty percent of preterm births directly result from preterm premature rupture of fetal membranes (PPROM). Clinical management currently proposes using a collagen plug to mechanically stop loss of amniotic fluid. Vitamin A and its active metabolite (retinoic acid) have well-known pro-healing properties and could thus make good candidates as a proposable adjuvant to this mechanical approach. Here we investigate the molecular mechanisms involved in the pro-healing properties of all-trans retinoic acid (atRA) in fetal membranes via an approach using an in vitro primary amniocyte wound model and transcriptomics...
October 18, 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27665369/fibromodulin-is-essential-for-fetal-type-scarless-cutaneous-wound-healing
#7
Zhong Zheng, Xinli Zhang, Catherine Dang, Steven Beanes, Grace X Chang, Yao Chen, Chen-Shuang Li, Kevin S Lee, Kang Ting, Chia Soo
In contrast to adult and late-gestation fetal skin wounds, which heal with scar, early-gestation fetal skin wounds display a remarkable capacity to heal scarlessly. Although the underlying mechanism of this transition from fetal-type scarless healing to adult-type healing with scar has been actively investigated for decades, in utero restoration of scarless healing in late-gestation fetal wounds has not been reported. In this study, using loss- and gain-of-function rodent fetal wound models, we identified that fibromodulin (Fm) is essential for fetal-type scarless wound healing...
November 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27568096/connexin-43-is-overexpressed-in-human-fetal-membrane-defects-after-fetoscopic-surgery
#8
David W Barrett, Anna L David, Christopher Thrasivoulou, Alvaro Mata, David L Becker, Alex C Engels, Jan A Deprest, Tina T Chowdhury
OBJECTIVE: We examined whether surgically induced membrane defects elevate connexin 43 (Cx43) expression in the wound edge of the amniotic membrane (AM) and drives structural changes in collagen that affects healing after fetoscopic surgery. METHOD: Cell morphology and collagen microstructure was investigated by scanning electron microscopy and second harmonic generation in fetal membranes taken from women who underwent fetal surgery. Immunofluoresence and real-time quantitative polymerase chain reaction was used to examine Cx43 expression in control and wound edge AM...
October 2016: Prenatal Diagnosis
https://www.readbyqxmd.com/read/27554806/hyaluronan-in-medical-practice
#9
Katarína Valachová, Nicola Volpi, Robert Stern, Ladislav Šoltés
Hyaluronan is the major extracellular matrix glycosaminoglycan polymer present in vertebrate tissues, with a molar mass that can reach several megaDaltons. It is particularly prominent in the matrix of tissues undergoing rapid turnover, in fetal tissues, and wherever regeneration and repair are occurring. Hyaluronan has highly varied biological functions often dependent on molar mass, however they are highly dependent on source of hyaluronan, its purity and nature of contaminants. Hyaluronan of highmolar- mass is known for its anti-angiogenic, anti-inflammatory and immunosuppressive properties, unlike hyaluronan of low-molar-mass that has the opposite effects...
August 24, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27530920/pigment-epithelium-derived-factor-upregulates-expression-of-vascular-endothelial-growth-factor-by-human-mesenchymal-stem-cells-possible-role-in-pedf-regulated-matrix-mineralization
#10
Feng Li, Gillian B Armstrong, Joyce Tombran-Tink, Christopher Niyibizi
Pigment epithelium-derived factor (PEDF) encoded by serpinf1 is a potent antiangiogenic factor found in a wide variety of fetal and adult tissues. Several reports have shown that lack of PEDF leads to osteogenesis imperfecta (OI) type VI whose hallmark is a defect in mineralization that leads to excessive osteoid build up that fails to mineralize. Because PEDF is antiangiogenic factor it would pose serious consequences on bone development and healing of fractures. To understand possible mechanisms by which PEDF plays a role in bone development and regulation of matrix mineralization, we determined the effects of exogenous PEDF on vascular endothelial growth factor (VEGF) expression by human mesenchymal stem cells (hMSCs) and mechanisms of its regulation by PEDF...
September 23, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27514438/fibroblast-growth-factor-and-vascular-endothelial-growth-factor-play-a-critical-role-in-endotheliogenesis-from-human-adipose-derived-stem-cells
#11
Sophia Khan, Miguel A Villalobos, Rachel L Choron, Shaohua Chang, Spencer A Brown, Jeffrey P Carpenter, Thomas N Tulenko, Ping Zhang
OBJECTIVE: Adipose-derived stem cells (ASCs) are a potential adult mesenchymal stem cell source for restoring endothelial function in patients with critical limb ischemia. Fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor (VEGF) play a major role in angiogenesis and wound healing. This study evaluated the effects of FGF and VEGF on the proliferation, migration, and potential endothelial differentiation of human ASCs with regards to their use as endothelial cell substitutes...
August 8, 2016: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/27510797/chemical-modification-of-extracellular-matrix-by-cold-atmospheric-plasma-generated-reactive-species-affects-chondrogenesis-and-bone-formation
#12
Peter Eisenhauer, Natalie Chernets, You Song, Danil Dobrynin, Nancy Pleshko, Marla J Steinbeck, Theresa A Freeman
The goal of this study was to investigate whether cold plasma generated by dielectric barrier discharge (DBD) modifies extracellular matrices (ECM) to influence chondrogenesis and endochondral ossification. Replacement of cartilage by bone during endochondral ossification is essential in fetal skeletal development, bone growth and fracture healing. Regulation of this process by the ECM occurs through matrix remodelling, involving a variety of cell attachment molecules and growth factors, which influence cell morphology and protein expression...
September 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27510554/the-methylome-and-transcriptome-of-fetal-skin-implications-for-scarless-healing
#13
Justyna Podolak-Popinigis, Anna Ronowicz, Monika Dmochowska, Agnieszka Jakubiak, Paweł Sachadyn
AIM: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16-17 day of gestation - the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition. MATERIALS & METHODS: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach. RESULTS & CONCLUSION: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after...
October 2016: Epigenomics
https://www.readbyqxmd.com/read/27450737/thymosin-%C3%AE-4-roles-in-development-repair-and-engineering-of-the-cardiovascular-system
#14
E D Marks, A Kumar
The burden of cardiovascular disease is a growing worldwide issue that demands attention. While many clinical trials are ongoing to test therapies for treating the heart after myocardial infarction (MI) and heart failure, there are few options doctors able to currently give patients to repair the heart. This eventually leads to decreased ventricular contractility and increased systemic disease, including vascular disorders that could result in stroke. Small peptides such as thymosin β4 (Tβ4) are upregulated in the cardiovascular niche during fetal development and after injuries such as MI, providing increased neovasculogenesis and paracrine signals for endogenous stem cell recruitment to aid in wound repair...
2016: Vitamins and Hormones
https://www.readbyqxmd.com/read/27420558/effect-of-amniotic-membrane-suspension-ams-and-amniotic-membrane-homogenate-amh-on-human-corneal-epithelial-cell-viability-migration-and-proliferation-in-vitro
#15
Ming-Feng Wu, Tanja Stachon, Achim Langenbucher, Berthold Seitz, Nóra Szentmáry
PURPOSE: To analyze the effects of different concentrations of amniotic membrane suspension (AMS) or amniotic membrane homogenate (AMH) on human corneal epithelial cell (HCEC) viability, migration and proliferation. METHODS: Amniotic membranes (AMs) of 13 placentas were prepared and stored at -80°C. For AMS preparation, following de-freezing, AM pieces were inserted in six-well plates and 5 ml Dulbecco's Modified Eagle's Medium (DMEM)/F12 (with 5% fetal bovine serum, FBS) per gram tissue was added for 96 h...
July 15, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27416953/proliferation-migration-and-expression-of-oral-mucosal-healing-related-genes-by-oral-fibroblasts-receiving-low-level-laser-therapy-after-inflammatory-cytokines-challenge
#16
Fernanda G Basso, Diana G Soares, Taisa N Pansani, Lais M Cardoso, Débora L Scheffel, Carlos Alberto de Souza Costa, Josimeri Hebling
BACKGROUND AND OBJECTIVES: Increased expression of inflammatory cytokines in the oral cavity has been related to the etiopathogenesis of oral mucositis and to delayed oral mucosal repair. Low-level laser therapy (LLLT) stimulates proliferation and migration of gingival fibroblasts, but the effects of specific inflammatory cytokines on oral mucosal cells and the modulation of these effects by LLLT have not been fully investigated. Therefore, this study investigated the effects of LLLT on oral fibroblasts after being challenged by oral-mucositis-related inflammatory cytokines...
July 15, 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/27406995/angiogenesis-and-wound-repair-when-enough-is-enough
#17
Luisa A DiPietro
All animals heal, and the ability to heal is requisite for human health. One aspect of repair that has always been considered to be essential for adequate healing is the creation of a new vasculature via angiogenesis. As adult skin wounds heal, a period of rapid and robust capillary growth creates a vascular bed that has many fold more capillaries than does normal tissue. Over time, most of the newly formed capillaries regress, resulting in a final vascular density similar to that of normal skin. Certainly, new capillaries are necessary to bring nutrients, immune cells, and oxygen to healing wounds...
July 12, 2016: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27393155/elastic-properties-of-woven-bone-effect-of-mineral-content-and-collagen-fibrils-orientation
#18
J García-Rodríguez, J Martínez-Reina
Woven bone is a type of tissue that forms mainly during fracture healing or fetal bone development. Its microstructure can be modeled as a composite with a matrix of mineral (hydroxyapatite) and inclusions of collagen fibrils with a more or less random orientation. In the present study, its elastic properties were estimated as a function of composition (degree of mineralization) and fibril orientation. A self-consistent homogenization scheme considering randomness of inclusions' orientation was used for this purpose...
July 8, 2016: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/27256396/scarless-wound-healing-finding-the-right-cells-and-signals
#19
REVIEW
Tripp Leavitt, Michael S Hu, Clement D Marshall, Leandra A Barnes, H Peter Lorenz, Michael T Longaker
From the moment we are born, every injury to the skin has the potential to form a scar, many of which can impair form and/or function. As such, scar management constitutes a billion-dollar industry. However, effectively promoting scarless wound healing remains an elusive goal. The complex interactions of wound healing contribute to our inability to recapitulate scarless wound repair as it occurs in nature, such as in fetal skin and the oral mucosa. However, many new advances have occurred in recent years, some of which have translated scientific findings from bench to bedside...
September 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27196106/macrophages-a-review-of-their-role-in-wound-healing-and-their-therapeutic-use
#20
Robert J Snyder, John Lantis, Robert S Kirsner, Vivek Shah, Michael Molyneaux, Marissa J Carter
Macrophages are mononuclear phagocytes established during embryogenesis and derived from the yolk sac or the fetal liver but also recruited from the blood and bone marrow under proliferative inflammatory conditions (such as tissue repair). Most importantly, they take on distinct phenotypes and functions crucial to healing upon localization in the wound. The objective of this review is to summarize recent findings in regard to the cellular mechanisms of macrophages and chronic wounds. Advances in the potential use of macrophage therapy have arisen based, in part, on the fact that early recruitment of macrophages is critical to wound healing...
July 2016: Wound Repair and Regeneration
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