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Fetal healing

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https://www.readbyqxmd.com/read/29809059/comparative-effectiveness-of-a-bilayered-living-cellular-construct-and-an-acellular-fetal-bovine-collagen-dressing-in-the-treatment-of-venous-leg-ulcers
#1
Michael L Sabolinski, Gary Gibbons
AIM: To compare the effectiveness of bilayered living cellular construct (BLCC) and an acellular fetal bovine collagen dressing (FBCD) for the treatment of venous leg ulcers. METHODS: Data from WoundExpert® (Net Health, PA, USA) was used to analyze 1021 refractory venous leg ulcers treated at 177 facilities. RESULTS: Kaplan-Meier analyses showed that BLCC (893 wounds) was superior to FBCD (128 wounds), p = 0.01 for: wound closure by weeks 12 (31 vs 25%), 24 (55 vs 43%) and 36 (68 vs 53%); reduction in time to wound closure of 37%, (19 vs 30 weeks); and improvement in the probability of healing by 45%...
May 29, 2018: Journal of Comparative Effectiveness Research
https://www.readbyqxmd.com/read/29786973/amniotic-fluid-lactic-acid-and-matrix-metalloproteinase-8-levels-at-the-time-of-fetal-surgery-for-a-spine-defect-association-with-subsequent-premature-preterm-rupture-of-membranes
#2
Antonio F Moron, Aikaterini Athanasiou, Mauricio Barbosa, Herbene J Milani, Stephanno G Sarmento, Sergio Cavalheiro, Steven S Witkin
OBJECTIVE: In utero fetal surgery to correct incomplete closure of the spinal cord lessens the extent of permanent damage but is associated with preterm premature rupture of membranes (pPROM). We determined if compounds in amniotic fluid (AF) collected at the time of surgery predicted subsequent development of pPROM. DESIGN: Prospective study. SETTING: Hospitals in Sao Paulo, Brazil. POPULATION: Twenty four consecutive pregnant women at 24-26 weeks gestation seen between February and October 2017 with a singleton pregnancy underwent in utero surgery to correct an open spinal defect in their fetus...
May 22, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29754319/responses-of-rainbow-trout-intestinal-epithelial-cells-to-different-kinds-of-nutritional-deprivation
#3
Patrick G Pumputis, Vivian R Dayeh, Lucy E J Lee, Phuc H Pham, Zhenzhen Liu, Senthuri Viththiyapaskaran, Niels C Bols
In order to develop an in vitro system to study the cell biology of starvation in the fish intestine, rainbow trout intestinal epithelial cells were subjected to three kinds of nutrient deprivation and evaluated for 7 days. The RTgutGC cell line was grown into monolayers in Leibovitz's basal medium supplemented with fetal bovine serum (L15/FBS) and then subjected to deprivation of serum (L15); of serum, amino acids, and vitamin (L15/ex); and of all nutrients (L15/salts). After 7 days of nutrient deprivation, the cells remained attached to the plastic surface as monolayers but changes were seen in shape, with the cells becoming more polygonal, actin and α-tubulin cytoskeleton organization, and in tight junction protein-1 (ZO-1) localization...
May 12, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29707732/a-pdms-device-coupled-with-culture-dish-for-in-vitro-cell-migration-assay
#4
Xiaoqing Lv, Zhaoxin Geng, Zhiyuan Fan, Shicai Wang, WeiHua Pei, Hongda Chen
Cell migration and invasion are important factors during tumor progression and metastasis. Wound-healing assay and the Boyden chamber assay are efficient tools to investigate tumor development because both of them could be applied to measure cell migration rate. Therefore, a simple and integrated polydimethylsiloxane (PDMS) device was developed for cell migration assay, which could perform quantitative evaluation of cell migration behaviors, especially for the wound-healing assay. The integrated device was composed of three units, which included cell culture dish, PDMS chamber, and wound generation mold...
April 30, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29673678/uterine-rupture-at-33rd-week-of-gestation-after-laparoscopic-myomectomy-with-signs-of-fetal-distress-a-case-report-and-review-of-literature
#5
Hiroyuki Yazawa, Kaoru Takiguchi, Fumihiro Ito, Keiya Fujimori
OBJECTIVE: We describe a case of uterine rupture (UR) during pregnancy after laparoscopic myomectomy (LM) and discuss the risk factors of UR. CASE REPORT: A 37-year-old woman with multiple myomas underwent laparoscopic myomectomy. Subserosal and intramural myomas were enucleated, and the myometrial wounds were repaired with single-layer suturing. Sixteen months after the operation, the patient conceived. At 33 weeks of gestation, emergency cesarean section was performed for the indication of fetal distress...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29648937/endothelial-wound-repair-of-the-organ-cultured-porcine-corneas
#6
Ingrida Smeringaiova, Stanislava Reinstein Merjava, Zbynek Stranak, Pavel Studeny, Jan Bednar, Katerina Jirsova
PURPOSE: To assess whether injured porcine endothelium of small and large corneoscleral disc differ in its reparative/regenerative capacity under various conditions of organ culture storage. MATERIAL AND METHODS: 166 paired porcine corneas were trephined to obtain tissues with diameter 12.0 mm and 17.5 mm (with area neighboring endothelial periphery). In tested discs, central endothelium was mechanically wounded. Density of live endothelial cells (LECD), percentage of dead cells (%DC), coefficient of variation and cell hexagonality were assessed in central and paracentral endothelium following 5- or 9-day incubation in medium with 2% or 10% fetal bovine serum...
April 12, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29625512/%C3%AE-arrestin2-affects-cardiac-progenitor-cell-survival-through-cell-mobility-and-tube-formation-in-severe-hypoxia
#7
Seul Ki Seo, Nari Kim, Ju Hee Lee, Sang Min Kim, Sang Yeub Lee, Jang Whan Bae, Kyung Kuk Hwang, Dong Woon Kim, Walter J Koch, Myeong Chan Cho
BACKGROUND AND OBJECTIVES: β-arrestin2 (β-arr2) basically regulates multiple signaling pathways in mammalian cells by desensitization and internalization of G-protein coupled receptors (GPCRs). We investigated impacts of β-arr2 on survival, mobility, and tube formation of cardiac progenitor cells (CPCs) obtained from wild-type (WT) mouse (CPC-WT), and β-arr2 knock-out (KO) mouse (CPC-KO) cultured in presence or absence of serum and oxygen as non-canonical roles in GPCR system. METHODS: CPCs were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 -based media containing fetal bovine serum and growth factors...
April 2018: Korean Circulation Journal
https://www.readbyqxmd.com/read/29579757/liberal-versus-indicated-maternal-oxygen-supplementation-in-labor-a-before-and-after-trial
#8
Nana-Ama E Ankumah, Sean C Blackwell, Mesk A Alrais, Farah H Amro, Rachel L Wiley, Patricia Heale, Maria Hutchinson, Baha M Sibai
BACKGROUND:  Although supplemental oxygen (SO2 ) is routinely administered to laboring gravidas, benefits and harms are not well studied. OBJECTIVE:  This article compares strategies of liberal versus indicated SO2 therapy during labor on cesarean delivery (CD) rate and neonatal outcomes. STUDY DESIGN:  A controlled, before-and-after trial of laboring women with term, singleton pregnancies. During an initial 8-week period, maternal SO2 was administered at the discretion of the provider followed by an 8-week period where SO2 was to be given only for protocol indications...
March 26, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29563288/age-associated-differences-in-infection-of-human-skin-in-the-scid-mouse-model-of-varicella-zoster-virus-pathogenesis
#9
Leigh Zerboni, Phillip Sung, Gordon Lee, Ann Arvin
Varicella-zoster virus (VZV) is the skin-tropic human alphaherpesvirus responsible for both varicella-zoster and herpes zoster. Varicella-zoster and herpes zoster skin lesions have similar morphologies, but herpes zoster occurs disproportionally in older individuals and is often associated with a more extensive local rash and severe zoster-related neuralgia. We hypothesized that skin aging could also influence the outcome of the anterograde axonal transport of VZV to skin. We utilized human skin xenografts maintained in immunodeficient (SCID) mice to study VZV-induced skin pathology in vivo in fetal and adult skin xenografts...
June 1, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29562775/restoring-in-vivo-like-membrane-lipidomics-promotes-exosome-trophic-behavior-from-human-placental-mesenchymal-stromal-stem-cells
#10
Claudia Cavallini, Chiara Zannini, Elena Olivi, Riccardo Tassinari, Valentina Taglioli, Martina Rossi, Paola Poggi, Alexandros Chatgilialoglu, Giuliana Simonazzi, Francesco Alviano, Laura Bonsi, Carlo Ventura
Human mesenchymal stem cells (hMSCs) are an effective tool in regenerative medicine notably for their intrinsic plentiful paracrine activity rather than differentiating properties. The hMSC secretome includes a wide spectrum of regulatory and trophic factors, encompassing several naked molecules as well as different kinds of extracellular vesicles (EVs). Among EVs, exosomes represent an intriguing population, able to shuttle proteins, transcription factors, and genetic materials, with a relevant role in cell-to-cell communication, modulating biological responses in recipient cells...
January 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29549768/production-scale-fibronectin-nanofibers-promote-wound-closure-and-tissue-repair-in-a-dermal-mouse-model
#11
Christophe O Chantre, Patrick H Campbell, Holly M Golecki, Adrian T Buganza, Andrew K Capulli, Leila F Deravi, Stephanie Dauth, Sean P Sheehy, Jeffrey A Paten, Karl Gledhill, Yanne S Doucet, Hasan E Abaci, Seungkuk Ahn, Benjamin D Pope, Jeffrey W Ruberti, Simon P Hoerstrup, Angela M Christiano, Kevin Kit Parker
Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (∼1...
June 2018: Biomaterials
https://www.readbyqxmd.com/read/29547658/the-corneal-epitheliotrophic-abilities-of-lyophilized-powder-form-human-platelet-lysates
#12
Lily Wei Chen, Chien-Jung Huang, Wen-Hui Tu, Chia-Ju Lu, Yi-Chen Sun, Szu-Yuan Lin, Wei-Li Chen
PURPOSE: To evaluate whether lyophilized human platelet lysate (HPL) powder can preserve the growth factor concentrations and epitheliotrophic properties of liquid HPL, and potentially be used as a clinically-friendly treatment option. METHODS: Two commercialized liquid HPLs, UltraGRO TM (Helios, Atlanta, GA) and PLTMax (Mill Creek, Rochester, MI), were obtained and converted to lyophilized powder. After redissolution, lyophilized powder HPLs were compared with liquid HPLs, as well as human peripheral serum (HPS) and fetal bovine serum (FBS) in liquid or redissolved lyophilized powder forms...
2018: PloS One
https://www.readbyqxmd.com/read/29525357/freshly-mixed-and-setting-calcium-silicate-cements-stimulate-human-dental-pulp-cells
#13
Mariano S Pedano, Xin Li, Shuchen Li, Zeyi Sun, Stevan M Cokic, Eveline Putzeys, Kumiko Yoshihara, Yashuhiro Yoshida, Zhi Chen, Kirsten Van Landuyt, Bart Van Meerbeek
OBJECTIVES: To evaluate the effect of the eluates from 3 freshly-mixed and setting hydraulic calcium-silicate cements (hCSCs) on human dental pulp cells (HDPCs) and to examine the effect of a newly developed hCSC containing phosphopullulan (PPL) on HDPCs. METHODS: Human dental pulp cells, previously characterized as mesenchymal stem cells, were used. To collect the eluates, disks occupying the whole surface of a 12-well plate were prepared using an experimental hCSC containing phosphopullulan (GC), Nex-Cem MTA (GC), Biodentine (Septodont) or a zinc-oxide (ZnO) eugenol cement (material-related negative control)...
May 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29491629/-in-vitro-cytotoxicity-and-apoptotic-assay-in-ht-29-cell-line-using-ficus-hispida-linn-leaves-extract
#14
Jayalalitha Sathiyamoorthy, Natarajan Sudhakar
Background: Ficus hispida Linn. (Family Moraceae ), well-known beneficial medicinal shrub, has been traditionally used for the treatment of various diseases such as leukoderma. Objective: The aim of the present study is to investigate the efficacy of F. hispida ethanolic leaves extract for antiproliferative, apoptotic, cell cycle blockade, and wound healing. Materials and Methods: F. hispida leaves extract was treated with colorectal adenocarcinoma cancer cell line HT29 for 24 h with control...
January 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29482651/effects-of-platelet-rich-plasma-on-the-activity-of-human-menstrual-blood-derived-stromal-cells-in-vitro
#15
Siwen Zhang, Pingping Li, Zhengwei Yuan, Jichun Tan
BACKGROUND: Human menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity. The convenience and non-invasiveness make MenSC a novel cell source for regenerative medicine applications. Platelet-rich plasma (PRP) contains abundant growth factors which are beneficial to wound healing. However, the influence of PRP on MenSCs remains elusive. Here, we evaluated the role of PRP in MenSCs proliferation and assessed the effects of PRP on endometrial receptivity regulation in vitro...
February 26, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29466789/two-port-fetoscopic-repair-of-myelomeningocele-in-fetal-lambs
#16
Lucie Guilbaud, Charles Garabedian, Nathalie Roux, Stéphanie Friszer, Ferdinand Dhombres, Raphaël Vialle, Zoobia Shah, Bettina Bessières, Federico Di Rocco, Michel Zerah, Jean-Marie Jouannic
OBJECTIVE: The aim of this study was to assess the feasibility and the effectiveness of a fetoscopic myelomeningocele (MMC) repair with a running single suture using a 2-port access in the sheep model. METHODS: Eighteen fetuses underwent surgical creation of a MMC defect at day 75. Fetuses were then randomized into 3 groups. Four fetuses remained untreated (control group). In the other 14 fetuses, a prenatal repair was performed at day 90: 7 fetuses had an open repair (oMMC), and 7 fetuses had a fetoscopic repair (fMMC) using a single-layer running suture through a 2-port access...
February 21, 2018: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/29459687/maturation-state-and-matrix-microstructure-regulate-interstitial-cell-migration-in-dense-connective-tissues
#17
Feini Qu, Qing Li, Xiao Wang, Xuan Cao, Miltiadis H Zgonis, John L Esterhai, Vivek B Shenoy, Lin Han, Robert L Mauck
Few regenerative approaches exist for the treatment of injuries to adult dense connective tissues. Compared to fetal tissues, adult connective tissues are hypocellular and show limited healing after injury. We hypothesized that robust repair can occur in fetal tissues with an immature extracellular matrix (ECM) that is conducive to cell migration, and that this process fails in adults due to the biophysical barriers imposed by the mature ECM. Using the knee meniscus as a platform, we evaluated the evolving micromechanics and microstructure of fetal and adult tissues, and interrogated the interstitial migratory capacity of adult meniscal cells through fetal and adult tissue microenvironments with or without partial enzymatic digestion...
February 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29420124/embryonic-skin-development-and-repair
#18
Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker
Fetal cutaneous wounds have the unique ability to completely regenerate wounded skin and heal without scarring. However, adult cutaneous wounds heal via a fibroproliferative response which results in the formation of a scar. Understanding the mechanism(s) of scarless wound healing leads to enormous clinical potential in facilitating an environment conducive to scarless healing in adult cutaneous wounds. This article reviews the embryonic development of the skin and outlines the structural and functional differences in adult and fetal wound healing phenotypes...
February 8, 2018: Organogenesis
https://www.readbyqxmd.com/read/29392093/mechanical-forces-in-cutaneous-wound-healing-emerging-therapies-to-minimize-scar-formation
#19
REVIEW
Leandra A Barnes, Clement D Marshall, Tripp Leavitt, Michael S Hu, Alessandra L Moore, Jennifer G Gonzalez, Michael T Longaker, Geoffrey C Gurtner
Significance: Excessive scarring is major clinical and financial burden in the United States. Improved therapies are necessary to reduce scarring, especially in patients affected by hypertrophic and keloid scars. Recent Advances: Advances in our understanding of mechanical forces in the wound environment enable us to target mechanical forces to minimize scar formation. Fetal wounds experience much lower resting stress when compared with adult wounds, and they heal without scars. Therapies that modulate mechanical forces in the wound environment are able to reduce scar size...
February 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29352858/cardiac-repair-by-epicardial-emt-current-targets-and-a-potential-role-for-the-primary-cilium
#20
REVIEW
Jessica N Blom, Qingping Feng
Despite therapeutic advances that have prolonged life, myocardial infarction (MI) remains a leading cause of death worldwide and imparts a significant economic burden. The advancement of treatments to improve cardiac repair post-MI requires the discovery of new targeted treatment strategies. Recent studies have highlighted the importance of the epicardial covering of the heart in both cardiac development and lower vertebrate cardiac regeneration. The epicardium serves as a source of cardiac cells including smooth muscle cells, endothelial cells and cardiac fibroblasts...
June 2018: Pharmacology & Therapeutics
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