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https://www.readbyqxmd.com/read/29648937/endothelial-wound-repair-of-the-organ-cultured-porcine-corneas
#1
Ingrida Smeringaiova, Stanislava Reinstein Merjava, Zbynek Stranak, Pavel Studeny, Jan Bednar, Katerina Jirsova
PURPOSE: To assess whether injured porcine endothelium of small and large corneoscleral disc differ in its reparative/regenerative capacity under various conditions of organ culture storage. MATERIAL AND METHODS: 166 paired porcine corneas were trephined to obtain tissues with diameter 12.0 mm and 17.5 mm (with area neighboring endothelial periphery). In tested discs, central endothelium was mechanically wounded. Density of live endothelial cells (LECD), percentage of dead cells (%DC), coefficient of variation and cell hexagonality were assessed in central and paracentral endothelium following 5- or 9-day incubation in medium with 2% or 10% fetal bovine serum...
April 12, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29625512/%C3%AE-arrestin2-affects-cardiac-progenitor-cell-survival-through-cell-mobility-and-tube-formation-in-severe-hypoxia
#2
Seul Ki Seo, Nari Kim, Ju Hee Lee, Sang Min Kim, Sang Yeub Lee, Jang Whan Bae, Kyung Kuk Hwang, Dong Woon Kim, Walter J Koch, Myeong Chan Cho
BACKGROUND AND OBJECTIVES: β-arrestin2 (β-arr2) basically regulates multiple signaling pathways in mammalian cells by desensitization and internalization of G-protein coupled receptors (GPCRs). We investigated impacts of β-arr2 on survival, mobility, and tube formation of cardiac progenitor cells (CPCs) obtained from wild-type (WT) mouse (CPC-WT), and β-arr2 knock-out (KO) mouse (CPC-KO) cultured in presence or absence of serum and oxygen as non-canonical roles in GPCR system. METHODS: CPCs were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 -based media containing fetal bovine serum and growth factors...
April 2018: Korean Circulation Journal
https://www.readbyqxmd.com/read/29579757/liberal-versus-indicated-maternal-oxygen-supplementation-in-labor-a-before-and-after-trial
#3
Nana-Ama E Ankumah, Sean C Blackwell, Mesk A Alrais, Farah H Amro, Rachel L Wiley, Patricia Heale, Maria Hutchinson, Baha M Sibai
BACKGROUND:  Although supplemental oxygen (SO2 ) is routinely administered to laboring gravidas, benefits and harms are not well studied. OBJECTIVE:  This article compares strategies of liberal versus indicated SO2 therapy during labor on cesarean delivery (CD) rate and neonatal outcomes. STUDY DESIGN:  A controlled, before-and-after trial of laboring women with term, singleton pregnancies. During an initial 8-week period, maternal SO2 was administered at the discretion of the provider followed by an 8-week period where SO2 was to be given only for protocol indications...
March 26, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29563288/age-associated-differences-in-infection-of-human-skin-in-the-scid-mouse-model-of-varicella-zoster-virus-pathogenesis
#4
Leigh Zerboni, Phillip Sung, Gordon Lee, Ann Arvin
Varicella-zoster virus (VZV) is the skin-tropic human alphaherpesvirus responsible for both varicella and herpes zoster. Varicella and herpes zoster skin lesions have similar morphology but herpes zoster occurs disproportionally in older individuals and is often associated with a more extensive local rash and severe zoster-related neuralgia. We hypothesized that skin aging could also influence the outcome of the anterograde axonal transport of VZV to skin. We utilized human skin xenografts maintained in immunodeficient (SCID) mice to study VZV-induced skin pathology in vivo in fetal and adult skin xenografts...
March 21, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29562775/restoring-in-vivo-like-membrane-lipidomics-promotes-exosome-trophic-behavior-from-human-placental-mesenchymal-stromal-stem-cells
#5
Claudia Cavallini, Chiara Zannini, Elena Olivi, Riccardo Tassinari, Valentina Taglioli, Martina Rossi, Paola Poggi, Alexandros Chatgilialoglu, Giuliana Simonazzi, Francesco Alviano, Laura Bonsi, Carlo Ventura
Human mesenchymal stem cells (hMSCs) are an effective tool in regenerative medicine notably for their intrinsic plentiful paracrine activity rather than differentiating properties. The hMSC secretome includes a wide spectrum of regulatory and trophic factors, encompassing several naked molecules as well as different kinds of extracellular vesicles (EVs). Among EVs, exosomes represent an intriguing population, able to shuttle proteins, transcription factors, and genetic materials, with a relevant role in cell-to-cell communication, modulating biological responses in recipient cells...
January 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29549768/production-scale-fibronectin-nanofibers-promote-wound-closure-and-tissue-repair-in-a-dermal-mouse-model
#6
Christophe O Chantre, Patrick H Campbell, Holly M Golecki, Adrian T Buganza, Andrew K Capulli, Leila F Deravi, Stephanie Dauth, Sean P Sheehy, Jeffrey A Paten, Karl Gledhill, Yanne S Doucet, Hasan E Abaci, Seungkuk Ahn, Benjamin D Pope, Jeffrey W Ruberti, Simon P Hoerstrup, Angela M Christiano, Kevin Kit Parker
Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (∼1...
March 5, 2018: Biomaterials
https://www.readbyqxmd.com/read/29547658/the-corneal-epitheliotrophic-abilities-of-lyophilized-powder-form-human-platelet-lysates
#7
Lily Wei Chen, Chien-Jung Huang, Wen-Hui Tu, Chia-Ju Lu, Yi-Chen Sun, Szu-Yuan Lin, Wei-Li Chen
PURPOSE: To evaluate whether lyophilized human platelet lysate (HPL) powder can preserve the growth factor concentrations and epitheliotrophic properties of liquid HPL, and potentially be used as a clinically-friendly treatment option. METHODS: Two commercialized liquid HPLs, UltraGRO TM (Helios, Atlanta, GA) and PLTMax (Mill Creek, Rochester, MI), were obtained and converted to lyophilized powder. After redissolution, lyophilized powder HPLs were compared with liquid HPLs, as well as human peripheral serum (HPS) and fetal bovine serum (FBS) in liquid or redissolved lyophilized powder forms...
2018: PloS One
https://www.readbyqxmd.com/read/29525357/freshly-mixed-and-setting-calcium-silicate-cements-stimulate-human-dental-pulp-cells
#8
Mariano S Pedano, Xin Li, Shuchen Li, Zeyi Sun, Stevan M Cokic, Eveline Putzeys, Kumiko Yoshihara, Yashuhiro Yoshida, Zhi Chen, Kirsten Van Landuyt, Bart Van Meerbeek
OBJECTIVES: To evaluate the effect of the eluates from 3 freshly-mixed and setting hydraulic calcium-silicate cements (hCSCs) on human dental pulp cells (HDPCs) and to examine the effect of a newly developed hCSC containing phosphopullulan (PPL) on HDPCs. METHODS: Human dental pulp cells, previously characterized as mesenchymal stem cells, were used. To collect the eluates, disks occupying the whole surface of a 12-well plate were prepared using an experimental hCSC containing phosphopullulan (GC), Nex-Cem MTA (GC), Biodentine (Septodont) or a zinc-oxide (ZnO) eugenol cement (material-related negative control)...
March 7, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29491629/-in-vitro-cytotoxicity-and-apoptotic-assay-in-ht-29-cell-line-using-ficus-hispida-linn-leaves-extract
#9
Jayalalitha Sathiyamoorthy, Natarajan Sudhakar
Background: Ficus hispida Linn. (Family Moraceae ), well-known beneficial medicinal shrub, has been traditionally used for the treatment of various diseases such as leukoderma. Objective: The aim of the present study is to investigate the efficacy of F. hispida ethanolic leaves extract for antiproliferative, apoptotic, cell cycle blockade, and wound healing. Materials and Methods: F. hispida leaves extract was treated with colorectal adenocarcinoma cancer cell line HT29 for 24 h with control...
January 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29482651/effects-of-platelet-rich-plasma-on-the-activity-of-human-menstrual-blood-derived-stromal-cells-in-vitro
#10
Siwen Zhang, Pingping Li, Zhengwei Yuan, Jichun Tan
BACKGROUND: Human menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity. The convenience and non-invasiveness make MenSC a novel cell source for regenerative medicine applications. Platelet-rich plasma (PRP) contains abundant growth factors which are beneficial to wound healing. However, the influence of PRP on MenSCs remains elusive. Here, we evaluated the role of PRP in MenSCs proliferation and assessed the effects of PRP on endometrial receptivity regulation in vitro...
February 26, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29466789/two-port-fetoscopic-repair-of-myelomeningocele-in-fetal-lambs
#11
Lucie Guilbaud, Charles Garabedian, Nathalie Roux, Stéphanie Friszer, Ferdinand Dhombres, Raphaël Vialle, Zoobia Shah, Bettina Bessières, Federico Di Rocco, Michel Zerah, Jean-Marie Jouannic
OBJECTIVE: The aim of this study was to assess the feasibility and the effectiveness of a fetoscopic myelomeningocele (MMC) repair with a running single suture using a 2-port access in the sheep model. METHODS: Eighteen fetuses underwent surgical creation of a MMC defect at day 75. Fetuses were then randomized into 3 groups. Four fetuses remained untreated (control group). In the other 14 fetuses, a prenatal repair was performed at day 90: 7 fetuses had an open repair (oMMC), and 7 fetuses had a fetoscopic repair (fMMC) using a single-layer running suture through a 2-port access...
February 21, 2018: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/29459687/maturation-state-and-matrix-microstructure-regulate-interstitial-cell-migration-in-dense-connective-tissues
#12
Feini Qu, Qing Li, Xiao Wang, Xuan Cao, Miltiadis H Zgonis, John L Esterhai, Vivek B Shenoy, Lin Han, Robert L Mauck
Few regenerative approaches exist for the treatment of injuries to adult dense connective tissues. Compared to fetal tissues, adult connective tissues are hypocellular and show limited healing after injury. We hypothesized that robust repair can occur in fetal tissues with an immature extracellular matrix (ECM) that is conducive to cell migration, and that this process fails in adults due to the biophysical barriers imposed by the mature ECM. Using the knee meniscus as a platform, we evaluated the evolving micromechanics and microstructure of fetal and adult tissues, and interrogated the interstitial migratory capacity of adult meniscal cells through fetal and adult tissue microenvironments with or without partial enzymatic digestion...
February 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29420124/embryonic-skin-development-and-repair
#13
Michael S Hu, Mimi R Borrelli, Wan Xing Hong, Samir Malhotra, Alexander T M Cheung, Ryan C Ransom, Robert C Rennert, Shane D Morrison, H Peter Lorenz, Michael T Longaker
Fetal cutaneous wounds have the unique ability to completely regenerate wounded skin and heal without scarring. However, adult cutaneous wounds heal via a fibroproliferative response which results in the formation of a scar. Understanding the mechanism(s) of scarless wound healing leads to enormous clinical potential in facilitating an environment conducive to scarless healing in adult cutaneous wounds. This article reviews the embryonic development of the skin and outlines the structural and functional differences in adult and fetal wound healing phenotypes...
February 8, 2018: Organogenesis
https://www.readbyqxmd.com/read/29392093/mechanical-forces-in-cutaneous-wound-healing-emerging-therapies-to-minimize-scar-formation
#14
REVIEW
Leandra A Barnes, Clement D Marshall, Tripp Leavitt, Michael S Hu, Alessandra L Moore, Jennifer G Gonzalez, Michael T Longaker, Geoffrey C Gurtner
Significance: Excessive scarring is major clinical and financial burden in the United States. Improved therapies are necessary to reduce scarring, especially in patients affected by hypertrophic and keloid scars. Recent Advances: Advances in our understanding of mechanical forces in the wound environment enable us to target mechanical forces to minimize scar formation. Fetal wounds experience much lower resting stress when compared with adult wounds, and they heal without scars. Therapies that modulate mechanical forces in the wound environment are able to reduce scar size...
February 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29352858/cardiac-repair-by-epicardial-emt-current-targets-and-a-potential-role-for-the-primary-cilium
#15
REVIEW
Jessica N Blom, Qingping Feng
Despite therapeutic advances that have prolonged life, myocardial infarction (MI) remains a leading cause of death worldwide and imparts a significant economic burden. The advancement of treatments to improve cardiac repair post-MI requires the discovery of new targeted treatment strategies. Recent studies have highlighted the importance of the epicardial covering of the heart in both cardiac development and lower vertebrate cardiac regeneration. The epicardium serves as a source of cardiac cells including smooth muscle cells, endothelial cells and cardiac fibroblasts...
January 17, 2018: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29344429/pathway-analysis-of-gene-expression-of-e14-versus-e18-fetal-fibroblasts
#16
Michael S Hu, Mimi R Borrelli, Michael Januszyk, Anna Luan, Samir Malhotra, Graham G Walmsley, Wan Xing Hong, Ruth Tevlin, Geoffrey C Gurtner, Michael T Longaker, Hermann P Lorenz
Objective: Fetuses early in gestation heal skin wounds without forming scars. The biological mechanisms behind this process are largely unknown. Fibroblasts, however, are cells known to be intimately involved in wound healing and scar formation. We examined fibroblasts in different stages of development to characterize differences in gene expression that may result in the switch from regenerative wound repair to repair with scarring. Approach: Fibroblasts were isolated and cultured from the back skin of BALB/c wild-type mouse fetuses at embryonic day (E)14 and E18 (n = 10)...
January 1, 2018: Advances in Wound Care
https://www.readbyqxmd.com/read/29335864/ethanol-extract-of-lycopodium-serratum-thunb-attenuates-lipopolysaccharide-induced-c6-glioma-cells-migration-via-matrix-metalloproteinase-9-expression
#17
Ju-Yeon Park, Hyuck Kim, Dong-Woo Lim, Jai-Eun Kim, Won-Hwan Park, Sun-Dong Park
OBJECTIVE: To elucidate how ethanol extract of L. serratum (ELS) could exert anti-migratory effects on glioma with the suppression of nuclear factor kappa B (NF-κB) downstream pathway. METHODS: Cell viability of ELS on C6 glioma was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) assay and 2',7'-dichloroflfluorescin diacetate (DCFH-DA) assay were applied to measure NO production and reactive oxygen species (ROS) generation on lipopolysaccharide (LPS)-induced C6 glioma cells...
January 15, 2018: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29332432/spontaneous-fetal-femoral-fracture-a-case-report-and-literature-review
#18
Mingming Yu, Dapeng Xu, Aiguo Zhang, Jun Shen
Spontaneous fetal femoral fractures are uncommon in the paediatric setting. The major clinical presentations of a spontaneous fetal femoral fracture are femoral angulation, shortness of the femur and even a marked fracture line. This case report describes a spontaneous fetal femoral fracture of the right femur, which was detected by routine ultrasonography during the 19th week of gestation in a 24-year-old woman. On routine follow-up visits, the angulation of the right femur in the fetus gradually improved...
March 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29330408/collagen-type-1-accelerates-healing-of-ruptured-fetal-membranes
#19
Haruta Mogami, Annavarapu Hari Kishore, R Ann Word
Preterm premature rupture of membranes (pPROM) is a major cause of preterm birth. Recently, extracellular matrix-directed treatment is applied for wound healing. Here, we used a pregnant mouse model to test the efficacy of collagen type 1 gel for healing of the prematurely ruptured fetal membranes. Although injection of PBS into the ruptured fetal membranes resulted in 40% closure, injection of collagen type 1 improved closure rates to 90% within 72 h. Macrophages of the M2 wound healing phenotype were entrapped in the collagen layer...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29323444/impact-of-new-diagnostic-criteria-for-gestational-diabetes
#20
Holly Sexton, Clare Heal, Jennifer Banks, Kathleen Braniff
AIM: In January 2015, the diagnostic and therapeutic criteria for gestational diabetes changed, with the goal of increasing the sensitivity of diagnosis and improving overall glycemic control, and thus reducing adverse pregnancy outcomes. Our primary aim was to evaluate the effect of the new guidelines on the incidence of diagnosis of gestational diabetes and the incidence of therapeutic interventions. Our secondary aim was to look at the incidence of adverse pregnancy outcomes. METHODS: A retrospective clinical audit was conducted at a regional hospital to compare the incidence of gestational diabetes, and the specific maternal and neonatal outcomes before and after the change in guidelines was implemented...
March 2018: Journal of Obstetrics and Gynaecology Research
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