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Fetal healing

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https://www.readbyqxmd.com/read/28319828/genistein-affects-proliferation-and-migration-of-bovine-oviductal-epithelial-cells
#1
Daniela C García, Pablo A Valdecantos, Dora C Miceli, Mariela Roldán-Olarte
Genistein is one of the most abundant isoflavones in soybean. This molecule induces cell cycle arrest and apoptosis in different normal and cancer cells. Genistein has been of considerable interest due to its adverse effects on bovine reproduction, altering estrous cycle, implantation and fetal development and producing subfertility or infertility. The objective of this work was to study the effects of genistein on the expression of selected genes involved in the regulation of cell cycle and apoptosis. Primary cultures of bovine oviductal epithelial cells (BOEC) were treated with different genistein concentrations (0...
March 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#2
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28238941/theoretical-and-practical-aspects-of-using-fetal-fibroblasts-for-skin-regeneration
#3
REVIEW
Meirong Li, Yali Zhao, Haojie Hao, Weidong Han, Xiaobing Fu
Cutaneous wounding in late-gestational fetal or postnatal humans results in scar formation without any skin appendages. Early or mid- gestational skin healing in humans is characterized by the absence of scaring in a process resembling regeneration. Tremendous cellular and molecular mechanisms contribute to this distinction, and fibroblasts play critical roles in scar or scarless wound healing. This review discussed the different repair mechanisms involved in wound healing of fibroblasts at different developmental stages and further confirmed that fetal fibroblast transplantation resulted in reduced scar healing in vivo...
February 24, 2017: Ageing Research Reviews
https://www.readbyqxmd.com/read/28216888/effect-of-absolute-from-hibiscus-syriacus-l-flower-on-wound-healing-in-keratinocytes
#4
Seok Won Yoon, Kang Pa Lee, Do-Yoon Kim, Dae Il Hwang, Kyung-Jong Won, Dae Won Lee, Hwan Myung Lee
BACKGROUND: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. OBJECTIVE: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. MATERIALS AND METHODS: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28205096/pharmacological-effects-of-asiatic-acid-in-glioblastoma-cells-under-hypoxia
#5
Flourina Kumar Thakor, Ka-Wai Wan, Philip John Welsby, Gail Welsby
Glioblastoma multiforme is the most common and malignant primary brain tumor in adults. Despite current treatment options including surgery followed by radiation and chemotherapy with temozolomide and cisplatin, the median survival rate remains low (<16 months). Combined with increasing drug resistance and the inability of some compounds to cross the blood-brain barrier, novel compounds are being sought for the treatment of this disease. Here, we aimed to examine the pharmacological effect of Asiatic acid (AA) in glioblastoma under hypoxia...
February 15, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28183603/chitosan-%C3%AE-1-3-glucan-hydroxyapatite-bone-scaffold-enhances-osteogenic-differentiation-through-tnf-%C3%AE-mediated-mechanism
#6
Agata Przekora, Grazyna Ginalska
The role of TNF-α in bone healing process is still unclear and controversial. Although it is commonly believed that TNF-α inhibits osteogenic differentiation, there are few reports that identified a crucial role of TNF-α in enhancing bone regeneration process. The aim of this study was to prove that novel chitosan/β-1,3-glucan/HA scaffold (chit/glu/HA) may promote osteogenic differentiation via TNF-α-mediated mechanism and an autocrine stimulation of osteoblasts. It was demonstrated that normal human fetal osteoblasts (hFOB 1...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28152010/comparison-of-corneal-epitheliotrophic-capacities-among-human-platelet-lysates-and-other-blood-derivatives
#7
Chien-Jung Huang, Yi-Chen Sun, Karen Christopher, Amy Shih-I Pai, Chia-Ju Lu, Fung-Rong Hu, Szu-Yuan Lin, Wei-Li Chen
PURPOSE: To evaluate the corneal epitheliotropic abilities of two commercialized human platelet lysates (HPLs) and to compare the results with other blood derivatives, including human peripheral serum (HPS) and bovine fetal serum (FBS). METHODS: In vitro, human corneal epithelial cells were incubated in various concentrations (0%, 3%, 5% and 10%) of blood derivatives. Two commercialized HPLs, including UltraGRO TM (Helios, Atlanta, GA) and PLTMax (Mill Creek, Rochester, MI), were tested and compared with HPS and FBS...
2017: PloS One
https://www.readbyqxmd.com/read/28135618/comparative-study-on-characterization-and-wound-healing-potential-of-goat-capra-hircus-mesenchymal-stem-cells-derived-from-fetal-origin-amniotic-fluid-and-adult-bone-marrow
#8
M D Pratheesh, Pawan K Dubey, Nitin E Gade, Amar Nath, T B Sivanarayanan, D N Madhu, Anjali Somal, Indu Baiju, T R Sreekumar, V L Gleeja, Irfan A Bhatt, Vikash Chandra, Amarpal, Bhaskar Sharma, G Saikumar, G Taru Sharma
Caprine amniotic fluid (cAF) and bone marrow cells (cBM) were isolated, expanded and phenotypically characterized by mesenchymal stem cells (MSCs) specific cell surface markers. Both cell types were compared for multilineage differentiation potential by flow cytometry using specific antibodies against lineage specific markers. Furthermore, in vitro expanded cAF-MSCs showed higher expression of trophic factors viz. VEGF and TGF-β1 as compared to cBM-MSCs. Full-skin thickness excisional wounds created on either side of the dorsal midline (thoracolumbar) of New Zealand White rabbits were randomly assigned to subcutaneous injection of either fetal origin cAF-MSCs (n=4) or adult cBM-MSCs (n=4) or sterile PBS (control, n=4)...
January 3, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28118667/restoration-of-mesenchymal-rpe-by-transcription-factor-mediated-reprogramming
#9
Ying-Hsuan Shih, Monte J Radeke, Carolyn M Radeke, Pete J Coffey
Purpose: Transforming growth factor β-mediated epithelial-to-mesenchymal transition (EMT) is a major component of the wound healing response and a negative determinant of retinal pigment epithelium (RPE) differentiation. We have shown previously that inhibition of TGFβ signaling restored the capacity of mesenchymal RPE to differentiate; however, the potential lessens with extensive passaging. We investigated TGFβ-independent mechanisms that regulate RPE differentiation following repetitive passaging...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28111421/combined-approaches-using-adverse-outcome-pathways-and-big-data-to-find-potential-diseases-associated-with-humidifier-disinfectant
#10
Jong-Han Leem, Kyu Hyuck Chung
According to previous survey, about two million of people were expected to suffer from toxic effects due to humidifier disinfectant (HD), regardless of healing or not. Extremely small group are recognized as HDs' victims. Up to now, previous research tried to focus on interstitial fibrosis on terminal bronchiole because it is specific finding, compared with other diseases. To figure out overall effects from HDs, we recommend adverse outcome pathways (AOPs) as new approach. Reactive oxygen species (ROS) generation, decreased T-cell and pro-inflammatory cytokine release from macrophage could be key events between the exposure to HDs and diseases...
2016: Environmental Health and Toxicology
https://www.readbyqxmd.com/read/28103990/-effects-of-human-amniotic-epithelial-stem-cells-derived-exosomes-on-healing-of-wound-with-full-thickness-skin-defect-in-rats
#11
B Zhao, G F Wu, Y J Zhang, W Zhang, F F Yang, D Xiao, K X Zeng, J H Shi, L L Su, D H Hu
Objective: To investigate the effects of human amniotic epithelial stem cells-derived exosomes on healing of wound with full-thickness skin defect in rats. Methods: (1) Human amniotic epithelial stem cells were isolated from the amnion tissue of 5 full-term pregnant women in Department of Obstetrics of our hospital by the method of trypsin digestion, and their morphology was observed. The third passage of cells were stained with rhodamine-phalloidin for cytoskeleton observation. The third passage of cells were identified with flow cytometry through the detection of expressions of cell surface markers CD29, CD31, CD34, CD90, CD105, SSEA3, SSEA4 and immunity-related marker human leukocyte antigen-D related site (HLA-DR)...
January 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28102936/unbiased-and-quantitative-proteomics-reveals-highly-increased-angiogenesis-induction-by-the-secretome-of-mesenchymal-stromal-cells-isolated-from-fetal-rather-than-adult-skin
#12
Massimiliano Gaetani, Cinzia Maria Chinnici, Anna Paola Carreca, Claudia Di Pasquale, Giandomenico Amico, Pier Giulio Conaldi
Scarless wound healing and functional regeneration are typical processes of the fetus, gradually lost during post-natal life, and maximally attributed to fetal skin tissue and induced by fetal skin fibroblasts. The latter have been successfully applied to post-natal wounds, with clear advantages compared with autologous dermis grafts or adult fibroblast applications. Our goal was to functionally identify and uncover key factors and mechanisms through the analysis of secretomes, the principal players in all cell therapies based on mesenchymal stromal cells (MSCs)...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28062236/enhanced-wound-healing-activity-of-desert-locust-schistocerca-gregaria-vs-shrimp-penaeus-monodon-chitosan-based-scaffolds
#13
Narguess H Marei, W El-Mazny, Aida El-Shaer, Kareem Dorri Zaki, Zahra S Hussein, Emtithal M Abd-El-Samie
Chitosan (CS) has received great attention in tissue engineering, especially in wound healing acceleration. In this study, chitin was isolated from desert locust (Schistocerca gregaria) and shrimp (Penaeus monodon) then deacetylated to chitosan. Then, chitosan was characterized by degree of deacetylation (DD), molecular weight (M.Wt), swelling index (SI), Fourier transform infrared (FTIR) and X ray diffraction (XRD). The chitosan was then casted into 2D scaffolds and was pictured using scanning electron microscope (SEM)...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28017598/biodegradable-poly-%C3%AE%C2%B5-caprolactone-microcarriers-for-efficient-production-of-human-mesenchymal-stromal-cells-and-secreted-cytokines-in-batch-and-fed-batch-bioreactors
#14
Alan Tin-Lun Lam, Jian Li, Jessica Pei-Wen Toh, Eileen Jia-Hui Sim, Allen Kuan-Liang Chen, Jerry Kok-Yen Chan, Mahesh Choolani, Shaul Reuveny, William R Birch, Steve Kah-Weng Oh
Large numbers of human mesenchymal stromal cells (MSCs) used for a variety of applications in tissue engineering and cell therapy can be generated by scalable expansion in a bioreactor using microcarriers (MCs) systems. However, the enzymatic digestion process needed to detach cells from the growth surface can affect cell viability and potentially the potency and differentiation efficiency. Thus, the main aim of our study was to develop biocompatible and biodegradable MCs that can support high MSC yields while maintaining their differentiation capability and potency...
December 22, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28004279/in-vitro-cultured-fetal-fibroblasts-have-myofibroblast-associated-characteristics-and-produce-a-fibrotic-like-environment-upon-stimulation-with-tgf-%C3%AE-1-is-there-a-thin-line-between-fetal-scarless-healing-and-fibrosis
#15
M Walraven, J J Akershoek, R H J Beelen, M M W Ulrich
Transforming growth factor-β (TGF-β) is a cytokine occurring in three isoforms with an important function in development and wound healing. In wound healing, prolonged TGF-β signaling results in myofibroblast differentiation and fibrosis. In contrast, the developing second-trimester fetal skin contains high levels of all three TGF-β isoforms but still has the intrinsic capacity to heal without scarring. Insight into TGF-β signal transduction during fetal wound healing might lead to methods to control the signaling pathway during adult wound healing...
March 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28003934/stability-enhancement-using-hyaluronic-acid-gels-for-delivery-of-human-fetal-progenitor-tenocytes
#16
A Grognuz, C Scaletta, A Farron, D P Pioletti, W Raffoul, L A Applegate
Tendon afflictions are very common, and their negative impact is high both at the workplace and in leisure activities. Tendinopathies are increasing in prevalence and can lead to tendon ruptures, where healing is a long process with outcomes that are often disappointing. Human fetal progenitor tenocytes (hFPTs) have been recently tested in vitro as a potential cell source to stimulate tendon regeneration. The aim of the present study was to compare different commercial hyaluronic acid (HA) gels, which could be used to resuspend hFPTs in a formulation that would allow for good delivery of the cells...
December 3, 2016: Cell Medicine
https://www.readbyqxmd.com/read/27966782/impact-of-cryopreservation-on-caprine-fetal-adnexa-derived-stem-cells-and-its-evaluation-for-growth-kinetics-phenotypic-characterization-and-wound-healing-potential-in-xenogenic-rat-model
#17
Anjali Somal, Irfan A Bhat, Indu Baiju, Anuj Pratap Singh, Bibhudatta S K Panda, Perumal Arumugam Desingu, Sriti Pandey, Mukesh Kumar Bharti, Amar Pal, G Saikumar, Vikash Chandra, G Taru Sharma
This study was conducted to know the impact of cryopreservation on caprine fetal adnexa derived mesenchymal stem cells (MSCs) on the basic stem cell characteristics. Gravid caprine uteri (2-3 months) were collected from local abattoir to derive {amniotic fluid (cAF), amniotic sac (cAS), Wharton's jelly (cWJ) and cord blood (cCB)} MSCs and expanded in vitro. Cells were vitrified at 3(rd) passage (P3) using 10% DMSO. Post thaw viability and cellular properties were assessed. Cells were expanded to determine growth kinetics, tri-lineage differentiation, localization and molecular expression of MSCs and pluripotency markers; thereafter, these cells were transplanted in the full-thickness (2 × 2cm(2) ) rat skin wound to determine their wound healing potential...
December 14, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27955656/means-of-enhancing-bone-fracture-healing-optimal-cell-source-isolation-methods-and-acoustic-stimulation
#18
Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes, Daniel B F Saris
BACKGROUND: The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to nonunion. Therefore, the aim of the present study was to assess the pool and biological functions of human mesenchymal stromal cells (hMSCs) isolated from different bone marrow locations of the lower limbs and to identify novel strategies to prime the cells prior to their use in bone fracture healing...
December 12, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27943665/combination-therapy-of-autologous-adipose-mesenchymal-stem-cell-enriched-high-density-lipoaspirate-and-topical-timolol-for-healing-chronic-wounds
#19
Larissa Larsen, Catherine N Tchanque-Fossuo, Farzam Gorouhi, David Bodreault, Chuong Nguyen, Robert W Crawford, Sara E Dahle, Thomas Whetzel, R Rivkah Isseroff
Chronic venous leg ulcers are profoundly debilitating and result in billions of health care expenditures. Thus, there is a quest for engineered and innovative approaches. Herein we present a 63 year-old patient with a 30-year history of venous stasis and left lower extremity ulcers, which have been refractory to standard of care, anticoagulation, and venous stripping. The medial ulcer was treated with transplantation of autologous adipose mesenchymal stem cell (AMSC)-enriched, high density lipoaspirate (HDL) on OASIS wound matrix, and compression therapy...
December 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27903619/interleukin-10-mediated-regenerative-postnatal-tissue-repair-is-dependent-on-regulation-of-hyaluronan-metabolism-via-fibroblast-specific-stat3-signaling
#20
Swathi Balaji, Xinyi Wang, Alice King, Louis D Le, Sukanta S Bhattacharya, Chad M Moles, Manish J Butte, Vinicio A de Jesus Perez, Kenneth W Liechty, Thomas N Wight, Timothy M Crombleholme, Paul L Bollyky, Sundeep G Keswani
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that regulates hyaluronan (HA) metabolism and drives adult fibroblasts to synthesize an HA-rich pericellular matrix, which mimics the fetal regenerative wound healing phenotype with reduced fibrosis. By using cre-lox-mediated novel, inducible, fibroblast-, keratinocyte-, and wound-specific STAT3-knockdown postnatal mice-plus syngeneic fibroblast cell-transplant models-we demonstrate that the regenerative effects of IL-10 in postnatal wounds are dependent on HA synthesis and fibroblast-specific STAT3-dependent signaling...
March 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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