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Amniotic membranes

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https://www.readbyqxmd.com/read/28426442/amniotic-membrane-use-for-management-of-corneal-limbal-stem-cell-deficiency
#1
Alfonso L Sabater, Victor L Perez
PURPOSE OF REVIEW: The current article reviews the most recent surgical techniques for management of corneal limbal stem cell deficiency (LSCD) using amniotic membrane tissue. RECENT FINDINGS: Early successes with amniotic membrane transplantation (AMT) for the treatment of ocular surface disorders have encouraged clinicians to investigate new applications. The use of AMT as a temporary patch in emergency cases in which LSCD may develop has considerably improved the prognosis of these patients...
April 19, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28421903/expression-of-matrix-metalloproteinase-9-and-its-substrate-level-in-patients-with-premature-rupture-of-membranes
#2
Ge Zuo, Jian-Xin Dong, Fang-Fei Zhao, Yan Chen
In this study, 30 case of patients with full-term premature membrane rupture and another 30 cases of full-term delivered subject without premature rupture of membranes (PROM) were selected to explore the relationship between premature membrane rupture with matrix metalloproteinase 9 (MMP-9) and its substrate level. Results showed the plasma zinc, MMP-9 in serum and amniotic fluid increased in patients with PROM; their type IV collagen in serum and foetal membrane decreased. Increased Zinc ion concentration results in increased concentration of MMP-9, a zinc-dependent enzyme; the degradation of type IV collagen by MMP-9 might be the potential mechanism of premature rupture of membranes in full-term pregnant women...
May 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28413063/amniotic-membrane-application-for-the-healing-of-chronic-wounds-and-ulcers
#3
Gregorio Castellanos, Ángel Bernabé-García, José M Moraleda, Francisco J Nicolás
Wound healing usually follows a predictable sequence and prognosis of events. Its evolutionary process is the result of a complicated interaction between patient-related factors, the wound, the treatment used and the skills and knowledge of the professionals who treat them. Only through a meticulous initial assessment of the wound is it possible to identify the factors that contribute to its complexity. The challenge for professionals will be to implement efficient therapies at the right time and in the most cost-efficient way in order to reduce associated problems, treat the symptoms and expectations of the patients and achieve adequate wound healing whenever possible...
April 10, 2017: Placenta
https://www.readbyqxmd.com/read/28412674/analysis-of-relevant-risk-factors-for-intrauterine-death-of-fetuses-in-the-third-trimester-of-pregnancy
#4
Yang Qiong, Yuan Yichong
Our study aimed to explore the relevant risk factors for intrauterine death of fetuses in the third trimester of pregnancy via a retrospective analysis. Then, 98 pregnant women with intrauterine death of fetuses in the third trimester of pregnancy were enrolled, who had undergone the induced labor of dead fetuses in our hospital from January, 2013 to January, 2015. By taking their disease conditions into considerations, methods of induced labor as softening of cervix with dinoprostone suppositories and amniotic infusion of ethacridine or oxytocin were performed, and the timely cesarean section for termination of pregnancy was performed...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28411944/differentiation-of-amniotic-epithelial-cells-into-various-liver-cell-types-and-potential-therapeutic-applications
#5
Maura Fanti, Roberto Gramignoli, Monica Serra, Erika Cadoni, Stephen C Strom, Fabio Marongiu
The aim of Regenerative Medicine is to replace or regenerate human cells, tissues or organs in order to restore normal function. Among all organs, the liver is endowed with remarkable regenerative capacity. Nonetheless, there are conditions in which this ability is impaired, and the use of isolated cells, including stem cells, is being considered as a possible therapeutic tool for the management of chronic hepatic disease. Placenta holds great promise for the field of regenerative medicine. It has long been used for the treatment of skin lesions and in ophthalmology, due to its ability to modulate inflammation and promote healing...
March 30, 2017: Placenta
https://www.readbyqxmd.com/read/28411000/meconium-aspiration-or-respiratory-distress-associated-with%C3%A2-meconium-stained-amniotic-fluid
#6
REVIEW
Nestor E Vain, Daniel G Batton
The designation meconium aspiration syndrome (MAS) reflects a spectrum of disorders in infants born with meconium-stained amniotic fluid, ranging from mild tachypnea to severe respiratory distress and significant mortality. The frequency of MAS is highest among infants with post-term gestation, thick meconium, and birth asphyxia. Pulmonary hypertension is an important component in severe cases. Prenatal hypopharyngeal suctioning and postnatal endotracheal intubation and suctioning of vigorous infants are not effective...
April 11, 2017: Seminars in Fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28409225/stromal-lenticule-transplantation-for-management-of-corneal-perforations-one-year-results
#7
Mohamed Samy Abd Elaziz, Adel Galal Zaky, Abdel Rahman El SaebaySarhan
PURPOSE: To study application of stromal lenticules extracted by femtolaser small incision lenticule extraction (SMILE) surgery as a surgical adjuvant to seal corneal perforations. METHODS: Corneal stromal lenticules obtained through SMILE surgery with central thickness 100 μm or more were fixed over corneal perforation sites using 10-0 nylon interrupted stitches with an overlying single layer of amniotic membrane. Seven patients who had been followed up for a minimum of 12 months were assessed using slit-lamp biomicroscopy, fluorescein stain, tonometry, and best spectacle-corrected visual acuity (BSCVA) measurements...
April 13, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28406800/simple-limbal-epithelial-transplantation
#8
Virender S Sangwan, John A H Sharp
PURPOSE OF REVIEW: Simple limbal epithelial transplant (SLET) is a technique for addressing limbal stem cell deficiency. Limbal tissue from a donor eye, typically the patient's fellow healthy eye, is transplanted onto an amniotic membrane attached to the surface of the diseased eye. SLET was developed to address limitations of other techniques. Specifically, the technical difficulty of ex-vivo expansion of cells required in some techniques and the larger amount of valuable limbal tissue harvested in techniques not relying on ex-vivo expansion...
April 12, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28403020/understanding-the-impact-of-preservation-methods-on-the-integrity-and-functionality-of-placental-allografts
#9
Amy Johnson, Alexandra Gyurdieva, Sandeep Dhall, Alla Danilkovitch, Yi Duan-Arnold
INTRODUCTION: Human placental membranes (hPMs) have a long history in treating burns and wounds. The composition of hPMs includes structural matrix, growth factors, and neonatal cells, all of which contribute to their regenerative potential. However, most hPM products are devitalized after dehydration and irradiation. We compared the functionality of single-layer viable cryopreserved human amniotic membrane (vCHAM) with multilayer devitalized dehydrated human amnion/chorion membrane (dHACM) in wound-relevant models to determine the effect of different processing methods on hPMs...
April 12, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28399069/conjunctival-limbal-autograft
#10
Sheraz M Daya
PURPOSE OF REVIEW: To provide an overview of conjunctival-limbal autografting (CLAU) for ocular surface rehabilitation with emphasis on more recent literature detailing outcomes of the procedure over the last 2 decades as well as technique variations and adjuvant techniques. RECENT FINDINGS: Limbal autografting initially described in 1964 by Barraquer and Strampelli and later popularized by Kenyon and Tseng is considered the best option for restoration of corneal phenotype in unilateral limbal stem-cell deficiency...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28388726/the-role-of-epithelial-to-mesenchymal-transition-in-human-amniotic-membrane-rupture
#11
Carla Janzen, Suvajit Sen, Margarida Y Y Lei, Marina Gagliardi de Assumpcao, John Challis, Gautam Chaudhuri
Context: Biochemical weakening of the amnion is a major factor preceding preterm premature rupture of membranes (PPROMs), leading to preterm birth. Activation of matrix metalloproteinases (MMPs) is known to play a key role in collagen degradation of the amnion; however, epithelial to mesenchymal transition (EMT) that is also induced by MMP activation has not been investigated as a mechanism for amnion weakening. Objective: To measure amniotic EMT associated with vaginal delivery (VD) compared with unlabored cesarean sections (CSs), and to assess changes in amniotic mechanical strength with pharmacologic inhibitors and inducers of EMT, thus testing the hypothesis that EMT is a key biochemical event that promotes amniotic rupture...
April 1, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28370981/in-vitro-assessment-of-a-novel-hypothermically-stored-amniotic-membrane-for-use-in-a-chronic-wound-environment
#12
John P McQuilling, Jeremy B Vines, Katie C Mowry
Chronic wounds require extensive healing time and place patients at risk of infection and amputation. Recently, a fresh hypothermically stored amniotic membrane (HSAM) was developed and has subsequently shown promise in its ability to effectively heal chronic wounds. The purpose of this study is to investigate the mechanisms of action that contribute to wound-healing responses observed with HSAM. A proteomic analysis was conducted on HSAM, measuring 25 growth factors specific to wound healing within the grafts...
March 29, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28366078/-the-myo-inositol-is-beneficial-in-the-therapy-of-pregnancy-with-insulin-dependent-type-2-diabetes-and-polycystic-ovary-syndrome
#13
Attila Kun, János Tornóczky
Authors would like to demonstrate the beneficial effect of myo-inositol supplementation in a pregnant woman with insulin-dependent type 2 diabetes mellitus and polycystic ovary syndrome. Insulin and metformin treatment could not achieve normalization of glucose homeostasis for 3 years, and hypoglycemic episodes were frequent. Myo-inositol and folic acid supplementation added to the basic treatment resulted in improved glucose levels in 2 months. At this time she became pregnant. During pregnancy serum glucose levels still improved in the next 2 months...
April 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/28365148/outcomes-after-flexor-tendon-repair-combined-with-the-application-of-human-amniotic-membrane-allograft
#14
Olli V Leppänen, Teemu Karjalainen, Harry Göransson, Annika Hakamäki, Jouni Havulinna, Jyrki Parkkinen, Jarkko Jokihaara
PURPOSE: Clinically proven methods to prevent adhesion formation after flexor tendon repair have not yet been established. The aim of this pilot study was to assess the feasibility of amniotic membrane allograft as a mechanical barrier to decrease adhesion formation. METHODS: Ten patients having flexor tendon injuries were planned to be recruited to the pilot study. The operative treatment consisted of tendon repair and fixation of amniotic membrane allograft around the repaired tendon...
March 29, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/28358839/frequency-and-clinical-significance-of-short-cervix-in-patients-with-preterm-premature-rupture-of-membranes
#15
Seung Mi Lee, Kyo Hoon Park, Eun Young Jung, Ji Ae Jang, Ha-Na Yoo
OBJECTIVE: Cervical length measurement has been uggested as a useful tool for predicting intra-amniotic infection/inflammation in preterm labor, but little information is available in the setting of preterm premature rupture of membranes (pPROM). We aimed to determine whether a short cervical length is independently associated with an increased risk of intra-amniotic infection or inflammation and impending preterm delivery in women with pPROM. METHODS: This was a retrospective cohort study involving 171 consecutive singleton pregnant women with pPROM (21+0-33+6 weeks' gestation), who underwent amniocentesis...
2017: PloS One
https://www.readbyqxmd.com/read/28347147/oxytocin-augmentation-in-spontaneously-laboring-nulliparous-women
#16
Nicole S Carlson, Elizabeth J Corwin, Nancy K Lowe
BACKGROUND: Synthetic oxytocin, the primary tool for labor augmentation, is less effective among obese women, leading to more unplanned cesarean deliveries for slow labor progress. It is not known if obese women require higher doses of oxytocin due to maternal, fetal, or labor factors related to maternal obesity. OBJECTIVES: This study had two main objectives: (1) examine the influence of maternal body mass index (BMI) on hourly doses of oxytocin from augmentation initiation until vaginal delivery in obese women; and (2) examine the influence of other maternal, fetal, and labor factors on hourly doses of oxytocin in obese women...
January 1, 2017: Biological Research for Nursing
https://www.readbyqxmd.com/read/28346794/comparative-study-of-xenobiotic-free-media-for-the-cultivation-of-human-limbal-epithelial-stem-progenitor-cells
#17
Sheyla González, Luxia Chen, Sophie X Deng
The culture of human limbal epithelial stem/progenitor cells (LSCs) in the presence of animal components poses the risk of cross-species contamination in clinical applications. We quantitatively compared different xenobiotic-free culture media for the cultivation of human LSCs. LSCs were cultured from 2 × 2 mm limbal tissue explants on denuded human amniotic membrane with different xenobiotic-free culture media: CnT-Prime (CnT-PR) supplemented with 0%, 1%, 5%, and 10% human serum (HS), embryonic stem cell medium (ESCM) alone or in combination with the standard supplemented hormonal epithelium medium (SHEM, control) at a 1:1 dilution ratio, and modified SHEM (mSHEM), in which cholera toxin and dimethyl sulfoxide (DMSO) were removed, isoproterenol was added, and the epidermal growth factor concentration was reduced...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28344763/utility-of-human-amniotic-membrane-allograft-in-re-epithelialization-of-the-nasal-tip
#18
D'Antonio C Dennis, Adam R Turnock, Collin Sutton, Bradley Chastant, Wesley B Vanderlan
Variations in skin thickness and contours pose significant challenges to reconstruction of the lower third of the nose. Human amniotic membrane allograft offers a potential alternative to tissue transfer in reconstruction of the lower third of the nose. We reviewed the procedure and photographs of a healthy 56-year-old male with a 22 × 18 mm lower third nasal defect involving full thickness skin and subcutaneous tissue. Following preparation for grafting, dehydrated human amniotic membrane was fashioned to the dimensions of the defect and applied...
October 2016: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/28343898/comparison-of-human-mesenchymal-stromal-cells-from-four-neonatal-tissues-amniotic-membrane-chorionic-membrane-placental-decidua-and-umbilical-cord
#19
Anelise Bergmann Araújo, Gabrielle Dias Salton, Juliana Monteiro Furlan, Natália Schneider, Melissa Helena Angeli, Álvaro Macedo Laureano, Lúcia Silla, Eduardo Pandolfi Passos, Ana Helena Paz
BACKGROUND: Mesenchymal stromal cells (MSCs) are being investigated as a potential alternative for cellular therapy. This study was designed to compare the biological characteristics of MSCs isolated from amniotic membrane (A-MSCs), chorionic membrane (C-MSCs), placental decidua (D-MSCs) and umbilical cord (UC-MSCs) to ascertain whether any one of these sources is superior to the others for cellular therapy purposes. METHODS: MSCs were isolated from amniotic membrane, chorionic membrane, umbilical cord and placental decidua...
March 23, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28335485/intra-amniotic-administration-of-raffinose-and-stachyose-affects-the-intestinal-brush-border-functionality-and-alters-gut-microflora-populations
#20
Sarina Pacifici, Jaehong Song, Cathy Zhang, Qiaoye Wang, Raymond P Glahn, Nikolai Kolba, Elad Tako
This study investigates the effectiveness of two types of prebiotics-stachyose and raffinose-which are present in staple food crops that are widely consumed in regions where dietary Fe deficiency is a health concern. The hypothesis is that these prebiotics will improve Fe status, intestinal functionality, and increase health-promoting bacterial populations in vivo (Gallus gallus). By using the intra-amniotic administration procedure, prebiotic treatment solutions were injected in ovo (day 17 of embryonic incubation) with varying concentrations of a 1...
March 19, 2017: Nutrients
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