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Amniotic membranes

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https://www.readbyqxmd.com/read/29333918/modified-second-stage-hughes-tarsoconjunctival-reconstruction-for-lower-eyelid-defects
#1
Shruti Aggarwal, Christopher T Shah, Maria Kirzhner
To describe a novel technique utilizing an amniotic membrane graft (AMT) to create the mucocutaneous portion of the lower eyelid margin in a modified Hughes eyelid reconstruction for secondary revision or prevention of a hyperemic, hypertrophic conjunctival margin with excessive discharge. This was a retrospective, non-comparative interventional study. Thirty consecutive patients who underwent a modified Hughes reconstruction were included. The first step of the reconstruction was performed in a standard fashion using a tarsoconjunctival flap from the ipsilateral upper eyelid...
January 15, 2018: Orbit
https://www.readbyqxmd.com/read/29330848/cultured-allogeneic-fibroblast-injection-versus-fibroblasts-cultured-on-amniotic-membrane-scaffold-for-dystrophic-epidermolysis-bullosa-treatment
#2
H Moravvej, F Abdollahimajd, M-H Naseh, Z Piravar, E Abolhasani, N Mozafari, H Niknejad
BACKGROUND: Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB). OBJECTIVE: To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with that of fibroblasts seeded on amniotic membrane scaffolds (FAMS) or standard wound care (SWC) with Vaseline gauze as controls. MATERIALS &METHODS: Seven patients were recruited, and seven wounds were assessed in each patient: three wounds were treated with injection of intradermal fibroblasts, three were treated with FAMS, and one was dressed with SWC...
January 12, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/29328072/immunomodulatory-effect-of-cd200-positive-human-placenta-derived-stem-cells-in-the-early-phase-of-stroke
#3
TaeHo Kong, Ji-Min Park, Ji Hyon Jang, C-Yoon Kim, Sang-Hun Bae, Yuri Choi, Yun-Hwa Jeong, Chul Kim, Sung Woon Chang, Joopyung Kim, Jisook Moon
Human placenta amniotic membrane-derived mesenchymal stem cells (AMSCs) regulate immune responses, and this property can be exploited to treat stroke patients via cell therapy. We investigated the expression profile of AMSCs cultured under hypoxic conditions and observed interesting expression changes in various genes involved in immune regulation. CD200, an anti-inflammatory factor and positive regulator of TGF-β, was more highly expressed under hypoxic conditions than normoxic conditions. Furthermore, AMSCs exhibited inhibition of pro-inflammatory cytokine expression in co-cultures with LPS-primed BV2 microglia, and this effect was decreased in CD200-silenced AMSCs...
January 12, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29321417/peripheral-ulcerative-keratitis-associated-with-granulomatosis-with-polyangiitis-emerging-despite-cyclophosphamide-successfully-treated-with-rituximab
#4
Yuya Fujita, Shoichi Fukui, Yushiro Endo, Sosuke Tsuji, Ayuko Takatani, Toshimasa Shimizu, Masataka Umeda, Ayako Nishino, Tomohiro Koga, Shin-Ya Kawashiri, Naoki Iwamoto, Kunihiro Ichinose, Mami Tamai, Hideki Nakamura, Tomoki Origuchi, Ryotaro Ueki, Masafumi Uematsu, Kaori Ishida, Kuniko Abe, Atsushi Kawakami
A 67-year-old Japanese man was diagnosed with granulomatosis with polyangiitis based on the presence of right maxillary sinusitis, proteinase 3 antineutrophil cytoplasmic antibody positivity, and right scleritis. A conjunctival biopsy specimen showed neutrophil-predominant infiltration around the vessels without granuloma. Because there was a risk of blindness, pulsed methylprednisolone and intravenous cyclophosphamide pulse therapy (IVCY) was started. However, it was ineffective, and peripheral ulcerative keratitis newly emerged...
January 11, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29316701/the-effect-of-cryopreserved-human-placental-tissues-on-biofilm-formation-of-wound-associated-pathogens
#5
Yong Mao, Anya Singh-Varma, Tyler Hoffman, Sandeep Dhall, Alla Danilkovitch, Joachim Kohn
Biofilm, a community of bacteria, is tolerant to antimicrobial agents and ubiquitous in chronic wounds. In a chronic DFU (Diabetic Foot Ulcers) clinical trial, the use of a human cryopreserved viable amniotic membrane (CVAM) resulted in a high rate of wound closure and reduction of wound-related infections. Our previous study demonstrated that CVAM possesses intrinsic antimicrobial activity against a spectrum of wound-associated bacteria under planktonic culture conditions. In this study, we evaluated the effect of CVAM and cryopreserved viable umbilical tissue (CVUT) on biofilm formation of S...
January 8, 2018: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29316210/fate-of-egg-proteins-during-the-development-of-columba-livia-domestica-embryo
#6
Seba Jamal Shbailat, Ibtisam Omar Aslan
The transfer of egg white into the yolk and consumption of yolk proteins by the embryo are largely unexplored in the pigeon Columba livia domestica. Here, we investigated the route of egg white transfer as well as the degradation and uptake of yolk proteins by the pigeon embryo. Initially, we tested the electrophoretic patterns of proteins in different egg compartments throughout development. Then, we used lysozyme as a reference protein to follow the egg white transfer, and we measured its activity using Micrococcus lysodeikticus as a substrate...
January 3, 2018: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/29315994/prediction-of-histological-chorioamnionitis-and-neonatal-and-infantile-outcomes-using-procalcitonin-in-the-umbilical-cord-blood-and-amniotic-fluid-at-birth
#7
Takashi Horinouchi, Toshiyuki Yoshizato, Yutaka Kozuma, Takaaki Shinagawa, Megumi Muto, Tsuyoshi Yamasaki, Daizo Hori, Kimio Ushijima
AIM: We aimed to clarify the usefulness of procalcitonin (PCT) in the evaluation of histological chorioamnionitis (CAM) and in the prediction of neonatal and infantile outcomes as a reference of interleukin-6 (IL-6). METHODS: Subjects were 36 singleton pregnant women delivered at 22-37 weeks' gestation due to threatened premature delivery and/or preterm premature rupture of membranes. Cases were classified into the CAM and non-CAM groups, according to Blanc's criteria...
January 5, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29313189/supportive-properties-of-basement-membrane-layer-of-human-amniotic-membrane-enable-development-of-tissue-engineering-applications
#8
Sonia Iranpour, Nasser Mahdavi-Shahri, Raheleh Miri, Halimeh Hasanzadeh, Hamid Reza Bidkhori, Hojjat Naderi-Meshkin, Ehsan Zahabi, Maryam M Matin
Human amniotic membrane (HAM) has been widely used as a natural scaffold in tissue engineering due to many of its unique biological properties such as providing growth factors, cytokines and tissue inhibitors of metalloproteinases. This study aimed at finding the most suitable and supportive layer of HAM as a delivery system for autologous or allogeneic cell transplantation. Three different layers of HAM were examined including basement membrane, epithelial and stromal layers. In order to prepare the basement membrane, de-epithelialization was performed using 0...
January 8, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29295667/in-vivo-evidence-of-inflammasome-activation-during-spontaneous-labor-at-term
#9
Bogdan Panaitescu, Roberto Romero, Nardhy Gomez-Lopez, Yi Xu, Yaozhu Leng, Eli Maymon, Percy Pacora, Offer Erez, Lami Yeo, Sonia S Hassan, Chaur-Dong Hsu
OBJECTIVE: Upon inflammasome activation, the adaptor protein of the inflammasome apoptosis-associated speck-like protein containing a CARD (ASC) forms intracellular specks, which can be released into the extracellular space. The objectives of this study were to investigate whether: 1) extracellular ASC is present in the amniotic fluid of women who delivered at term; 2) amniotic fluid ASC concentrations are greater in women who underwent spontaneous labor at term than in those who delivered at term in the absence of labor; and 3) amniotic epithelial and mesenchymal cells can form intracellular ASC specks invitro...
January 2, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29286443/reprogramming-primary-amniotic-fluid-and-membrane-cells-to-pluripotency-in-xeno-free-conditions
#10
Jaroslav Slamecka, Javier Laurini, Troy Shirley, Simon Philipp Hoerstrup, Benedikt Weber, Laurie Owen, Steven McClellan
Autologous cell-based therapies got a step closer to reality with the introduction of induced pluripotent stem cells. Fetal stem cells, such as amniotic fluid and membrane mesenchymal stem cells, represent a unique type of undifferentiated cells with promise in tissue engineering and for reprogramming into iPSC for future pediatric interventions and stem cell banking. The protocol presented here describes an optimized procedure for extracting and culturing primary amniotic fluid and membrane mesenchymal stem cells and generating episomal induced pluripotent stem cells from these cells in fully chemically defined culture conditions utilizing human recombinant vitronectin and the E8 medium...
November 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29280532/sludge-reflects-intra-amniotic-inflammation-with-or-without-microorganisms
#11
Noriko Yoneda, Satoshi Yoneda, Hideki Niimi, Masami Ito, Kaori Fukuta, Tomohiro Ueno, Mika Ito, Arihiro Shiozaki, Mika Kigawa, Isao Kitajima, Shigeru Saito
PROBLEM: To investigate whether amniotic fluid (AF) "sludge" in patients with preterm labor (PTL) with intact membranes is related to intra-amniotic infection or inflammation. METHOD OF STUDY: 105 PTL patients before 29 weeks' gestation were enrolled. AF "sludge" was evaluated by transvaginal sonography. Microorganisms were identified in AF by our newly established PCR method using a eukaryote-made thermostable DNA polymerase. RESULTS: AF "sludge" was present in 18...
December 27, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29279602/new-therapeutic-perceptions-in-a-patient-with-complicated-herpes-simplex-virus-1-keratitis-a-case-report-and-review-of-the-literature
#12
REVIEW
Dimitrios Kalogeropoulos, Aliki Geka, Konstantinos Malamos, Maria Kanari, Chris Kalogeropoulos
BACKGROUND Keratitis caused by herpes simplex virus (HSV) can have detrimental effects on the cornea leading to loss of vision. Modern therapies can contribute to the prevention of anatomical and functional damage. CASE REPORT An 80-year-old male with complicated HSV-1 keratitis of the left eye (confirmed diagnosis after microbiological investigation) presented three months after antiviral treatment with corneal blurring, severe epitheliopathy, thinning of the stroma, and neovascularization. At the time he was referred, the visual acuity of his left eye was very low, as he could only count fingers at a one-foot distance...
December 27, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/29260497/equine-pericardium-for-tectonic-globe-repair
#13
Konstantinos Droutsas, Dimitrios Papaconstantinou, Ilias Georgalas, Georgios Kymionis, Konstantinos Andreanos, Elli Stamou, Nikolaos Mamas
PURPOSE: To describe the use of equine pericardium as an off-label temporary emergency treatment of scleral and corneal perforations. METHODS: Three eyes of two male patients aged 34 and 38 years were included, i.e. a case with a history of severe bilateral thermal burn undergoing phacoemulsification complicated by tearing of the main port causing iris exposure and a patient with bilateral corneal perforation secondary to non-infectious corneal melt due to presumed ocular non-steroid anti-inflammatory drug abuse...
December 19, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29260110/surgical-treatment-outcome-of-medically-refractory-huge-giant-papillary-conjunctivitis
#14
Yien Lai, Gangadhara Sundar, Manotosh Ray
Purpose: To compare the surgical outcome of excision of giant papillae with and without amniotic membrane in a patient with bilateral medically refractory giant papillary conjunctivitis (GPC). Observations: 27-year-old Chinese lady presented with bilateral itchy eyes, discomfort and fullness of upper lids for past two years. She was a long-term contact lens user but stopped completely 2 years ago. Not a known atopic, she had unusually large giant papillae involving both upper tarsal conjunctiva...
December 2017: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29260079/a-stepping-stone-in-treating-dendritic-keratitis
#15
Hosam Sheha, Sean Tighe, Anny M S Cheng, Scheffer C G Tseng
Purpose: To report the outcome of self-retained amniotic membrane after debridement in recurrent dendritic keratitis. Observations: A 70-year-old female with a recurrent dendritic corneal ulcer received debridement followed by placement of self-retained amniotic membrane. Five days after treatment, the patient experienced a complete resolution of symptoms, marked reduction of inflammation, complete re-epithelialization of the cornea and improvement of visual acuity...
September 2017: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29247317/bilayer-amniotic-membrane-nano-fibrous-fibroin-scaffold-promotes-differentiation-capability-of-menstrual-blood-stem-cells-into-keratinocyte-like-cells
#16
Maryam Fard, Maryam Akhavan-Tavakoli, Sayeh Khanjani, Sona Zare, Haleh Edalatkhah, Shaghayegh Arasteh, Davood Mehrabani, Amir-Hassan Zarnani, Somaieh Kazemnejad, Reza Shirazi
The skin provides a dynamic barrier separating and protecting human body from the exterior world, and then immediate repair and rebuilding of the epidermal barrier is crucial after wound and injury. Wound healing without scars and complete regeneration of skin tissue still remain as a clinical challenge. The demand to engineer scaffolds that actively promote regeneration of damaged areas of the skin has been increased. In this study, menstrual blood-derived stem cells (MenSCs) have been induced to differentiate into keratinocytes-like cells in the presence of human foreskin-derived keratinocytes on a bilayer scaffold based on amniotic membrane and silk fibroin...
December 15, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29245335/spontaneous-corneal-perforation-in-terrien-s-marginal-degeneration-in-childhood-a-case-report
#17
Junkyu Chung, Kyung Hyun Jin, Jaheon Kang, Tae Gi Kim
RATIONALE: Terrien's marginal corneal degeneration (TMD) is characterized by progressive peripheral corneal thinning. It appears primarily above the age of 40 years and is rare at younger ages. Spontaneous corneal perforation in TMD is a rare, but serious complication that may occur in childhood. PATIENT CONCERNS: This review presents the case of a 16-year-old girl presented with blurred vision in the right eye on awakening in the morning. Slit-lamp examination revealed superior corneal thinning with a corneal perforation...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29241965/perinatal-hemorrhage-from-ulceration-of-the-umbilical-cord-a-potentially-catastrophic-association-with-duodenal-and-jejunal-obstruction
#18
Danielle Aronowitz, Stephen Dolgin, Eran Bornstein, Amanda Little-Richardson, Jaya Bajaj, Alex Williamson, Esperanza Naves-Ruiz, Morris Edelman
PURPOSE: The purpose of this study is to review published reports and contribute new cases of umbilical cord ulceration (UCU) with perinatal hemorrhage into the amniotic cavity in the setting of duodenal or jejunal obstruction because knowledge of this sequence is poorly disseminated and could be lifesaving. METHODS: Published reports of UCU with hemorrhage associated with congenital duodenal or jejunal obstruction were reviewed. Chart review was conducted for the cases encountered at our institutions between January 2008 and March 2017...
November 21, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29239057/in-which-preterm-labor-patients-is-intravenous-maintenance-tocolysis-effective
#19
Satoshi Yoneda, Noriko Yoneda, Kaori Fukuta, Tomoko Shima, Akitoshi Nakashima, Arihiro Shiozaki, Osamu Yoshino, Mika Kigawa, Taketoshi Yoshida, Shigeru Saito
AIM: We evaluated whether maintenance tocolysis (intravenous ritodrine hydrochloride and/or magnesium sulfate) was effective in cases of spontaneous preterm labor with intact membranes. METHODS: One hundred and thirty preterm labor patients who reached 36 weeks of gestation by maintenance tocolysis were selected. Immediate delivery (ID) after ceasing maintenance tocolysis was defined as an 'effective case'. The correlated factors between ID and no immediate delivery (NID) were statistically analyzed...
December 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29234870/-good-practice-procedures-for-acquisition-and-preparation-of-cryopreserved-human-amniotic-membranes-from-donor-placentas
#20
REVIEW
H Thomasen, J Schroeter, T Reinhard, B Seitz, K-P Steuhl, D Meller
Within the framework of obtaining a valid authorization for tissue preparation of cryopreserved human amniotic membranes at the Paul Ehrlich Institute, pursuant to § 21a paragraph 1 of the German Medicines Act (AMG), parts of the existing good practice procedures for acquisition of cryopreserved human amniotic membranes from donor placentas were reviewed and supplemented by new knowledge. The present good practice procedures were formulated in cooperation with members of the section for tissue transplantation and biotechnology of the German Ophthalmological Society...
December 12, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
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