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Polymerase zeta

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https://www.readbyqxmd.com/read/28704436/platinum-nanoparticles-induce-damage-to-dna-and-inhibit-dna-replication
#1
Lukas Nejdl, Jiri Kudr, Amitava Moulick, Dagmar Hegerova, Branislav Ruttkay-Nedecky, Jaromir Gumulec, Kristyna Cihalova, Kristyna Smerkova, Simona Dostalova, Sona Krizkova, Marie Novotna, Pavel Kopel, Vojtech Adam
Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry...
2017: PloS One
https://www.readbyqxmd.com/read/28631559/pkc%C3%AE-in-prostate-cancer-cells-represses-the-recruitment-and-m2-polarization-of-macrophages-in-the-prostate-cancer-microenvironment
#2
Hui-Hui Fan, Ling Li, Yu-Ming Zhang, Jie Yang, Mao-Cheng Li, Fang-Yin Zeng, Fan Deng
Tumor-associated macrophages are key regulators of the complex interplay between tumor and tumor microenvironment. M2 Macrophages, one type of tumor-associated macrophages, are involved in prostate cancer growth and progression. Protein kinase C zeta has been shown to suppress prostate cancer cell growth, invasion, and metastasis as a tumor suppressor; however, its role in chemotaxis and activation of tumor-associated macrophages remains unclear. Here, we investigated the role of protein kinase C zeta of prostate cancer cells in regulation of macrophage chemotaxis and M2 phenotype activation...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28521674/sorbitane-monostearate-and-cholesterol-based-niosomes-for-oral-delivery-of-telmisartan-from-bench-to-preclinical-implementation-in-wistar-albino-rats
#3
Abdul Ahad, Mohammad Raish, Fahad I Al-Jenoobi, Abdullah M Al-Mohizea
BACKGROUND: Niosomes are non-ionic surfactant vesicles used as drug carriers for encapsulating both hydrophobic and hydrophilic drugs. The aim of the present study was to prepare and characterize niosomes formulations for oral delivery of telmisartan and evaluated for its antihypertensive activity. METHOD: Telmisartan loaded niosomes were prepared using thin film hydration method by varying the Span 60 and cholesterol at several molar ratios and characterized for vesicles size, polydispersity index, zeta potential, entrapment efficiency...
May 18, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/28410879/determination-of-reference-genes-that-are-independent-of-feeding-rhythms-for-circadian-studies-of-mouse-metabolic-tissues
#4
Reiko Nakao, Hiroki Okauchi, Chiaki Hashimoto, Naoyuki Wada, Katsutaka Oishi
Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis is a popular method for the measurement of mRNA expression level and is a critical tool for basic research. The identification of suitable reference genes that are stable and not affected by experimental conditions is a critical step in the accurate normalization of RT-PCR. On the other hand, the levels of numerous transcripts exhibit circadian oscillation in various peripheral tissues and it is thought to be regulated by feeding rhythms in addition to the molecular circadian clock...
April 5, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28315051/development-of-antibody-modified-chitosan-nanoparticles-for-the-targeted-delivery-of-sirna-across-the-blood-brain-barrier-as-a-strategy-for-inhibiting-hiv-replication-in-astrocytes
#5
Jijin Gu, Karam Al-Bayati, Emmanuel A Ho
RNA interference (RNAi)-mediated gene silencing offers a novel treatment and prevention strategy for human immunodeficiency virus (HIV) infection. HIV was found to infect and replicate in human brain cells and can cause neuroinfections and neurological deterioration. We designed dual-antibody-modified chitosan/small interfering RNA (siRNA) nanoparticles to deliver siRNA across the blood-brain barrier (BBB) targeting HIV-infected brain astrocytes as a strategy for inhibiting HIV replication. We hypothesized that transferrin antibody and bradykinin B2 antibody could specifically bind to the transferrin receptor (TfR) and bradykinin B2 receptor (B2R), respectively, and deliver siRNA across the BBB into astrocytes as potential targeting ligands...
August 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/27920500/design-synthesis-and-evaluation-of-vegf-sirna-crs-as-a-novel-vector-for-gene-delivery
#6
Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Small interfering RNA (siRNA) delivery is a prospective method in gene therapy, but it has application limitations such as negative charge, water solubility and high molecular weight. In this study, a safe and efficient nano-vector, CRS, was designed and synthesized to facilitate siRNA delivery. Physical and chemical properties of VEGF-siRNA/CRS were characterized by methods including scanning electron microscopy (SEM), transmission electron microscopy, zeta potential (ζ) measurement, drug-releasing rate measurement, gel electrophoresis and confocal microscopy...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27895484/the-effect-of-dexamethasone-cell-penetrating-peptide-nanoparticles-on-gene-delivery-for-inner-ear-therapy
#7
Ji Young Yoon, Keum-Jin Yang, Shi-Nae Park, Dong-Kee Kim, Jong-Duk Kim
Dexamethasone (Dex)-loaded PHEA-g-C18-Arg8 (PCA) nanoparticles (PCA/Dex) were developed for the delivery of genes to determine the synergistic effect of Dex on gene expression. The cationic PCA nanoparticles were self-assembled to create cationic micelles containing an octadecylamine (C18) core with Dex and an arginine 8 (Arg8) peptide shell for electrostatic complexation with nucleic acids (connexin 26 [Cx26] siRNA, green fluorescent protein [GFP] DNA or brain-derived neurotrophic factor [BDNF] pDNA). The PCA/Dex nanoparticles conjugated with Arg8, a cell-penetrating peptide that enhances permeability through a round window membrane in the inner ear for gene delivery, exhibited high uptake efficiency in HEI-OC1 cells...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27853365/gene-silencing-effects-of-anti-survivin-sirna-delivered-by-rgdv-functionalized-nanodiamond-carrier-in-the-breast-carcinoma-cell-line-mcf-7
#8
Yanzhao Bi, Yifan Zhang, Chunying Cui, Lulu Ren, Xueyun Jiang
Nanodiamond (ND) is a renowned material in nonviral small interfering RNA (siRNA) carrier field due to its unique physical, chemical, and biological properties. In our previous work, it was proven that ND could deliver siRNA into cells efficiently and downregulate the expression of desired protein. However, synthesizing a high-efficient tumor-targeting carrier using ND is still a challenge. In this study, a novel carrier, NDCONH(CH2)2NH-VDGR, was synthesized for siRNA delivery, and its properties were characterized with methods including Fourier transform infrared spectrometry, transmission electron microscopy, scanning electron microscopy, gel retardation assay, differential scanning calorimetry, confocal microscopy, releasing test, real-time polymerase chain reaction (PCR) assay, enzyme-linked immunosorbent assay (ELISA), flow cytometry, cytotoxicity assay, and gene-silencing efficacy assay in vitro and in vivo...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27602326/evaluation-of-plc%C3%AE-and-pawp-expression-in-globozoospermic-individuals
#9
Majid Kamali-Dolat Abadi, Marziyeh Tavalaee, Abdolhossein Shahverdi, Mohammad Hossein Nasr-Esfahani
OBJECTIVE: Globozoospermia is a rare type of teratozoospermia with incidence of 0.1% among infertile individuals. Phospholipase C zeta (PLCζ) and postacrosomal sheath WW domain binding protein (PAWP) are the main candidates in sperm taking responsibility for oocyte activation during fertilization. Therefore, we aimed to evaluate the expression of these two genes at RNA and protein levels in globozoospermic individuals and compare the results with fertile individuals. MATERIALS AND METHODS: In this experimental study, semen samples of 21 infertile men with globozoospermia and 25 fertile men were collected...
2016: Cell Journal
https://www.readbyqxmd.com/read/27488450/mad2b-promotes-tubular-epithelial-to-mesenchymal-transition-and-renal-tubulointerstitial-fibrosis-via-skp2
#10
Hui Tang, Di Fan, Chun-Tao Lei, Chen Ye, Pan Gao, Shan Chen, Xian-Fang Meng, Hua Su, Chun Zhang
The mitotic arrest deficient protein MAD2B is a well-defined anaphase-promoting complex/cyclosome (APC/C) inhibitor and a small subunit of DNA polymerase zeta. It is critical for mitotic control and DNA repair. However, the pathological role of MAD2B in kidney diseases has not been fully elucidated. In the present study, we aim to explore the role of MAD2B in the pathogenesis of renal tubulointerstitial fibrosis (TIF) and the underlying mechanism. By immunofluorescence and immunohistochemistry, we found an obvious MAD2B enhancement in tubular area of TIF patients and unilateral ureteral obstruction (UUO) mice...
November 2016: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/27458205/histone-h2b-mono-ubiquitylation-maintains-genomic-integrity-at-stalled-replication-forks
#11
Matthew R Northam, Kelly M Trujillo
Histone modifications play an important role in regulating access to DNA for transcription, DNA repair and DNA replication. A central player in these events is the mono-ubiquitylation of histone H2B (H2Bub1), which has been shown to regulate nucleosome dynamics. Previously, it was shown that H2Bub1 was important for nucleosome assembly onto nascent DNA at active replication forks. In the absence of H2Bub1, incomplete chromatin structures resulted in several replication defects. Here, we report new evidence, which shows that loss of H2Bub1 contributes to genomic instability in yeast...
November 2, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27275415/sustained-release-genistein-from-nanostructured-lipid-carrier-suppresses-human-lens-epithelial-cell-growth
#12
Jin-Lu Liu, Wen-Ji Zhang, Xue-Dong Li, Na Yang, Wei-San Pan, Jun Kong, Jin-Song Zhang
AIM: To design and investigate the efficacy of a modified nanostructured lipid carrier loaded with genistein (Gen-NLC) to inhibit human lens epithelial cells (HLECs) proliferation. METHODS: Gen-NLC was made by melt emulsification method. The morphology, particle size (PS), zeta potentials (ZP), encapsulation efficiency (EE) and in vitro release were characterized. The inhibition effect of nanostructured lipid carrier (NLC), genistein (Gen) and Gen-NLC on HLECs proliferation was evaluated by cell counting kit-8 (CCK-8) assay, gene and protein expression of the proliferation marker Ki67 were evaluated with real-time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence analyses...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27199558/phytosynthesis-of-silver-nanoparticles-using-artemisia-marschalliana-sprengel-aerial-part-extract-and-assessment-of-their-antioxidant-anticancer-and-antibacterial-properties
#13
Soheil Salehi, Seyed Ataollah Sadat Shandiz, Farinaz Ghanbar, Mohammad Raouf Darvish, Mehdi Shafiee Ardestani, Amir Mirzaie, Mohsen Jafari
A rapid phytosynthesis of silver nanoparticles (AgNPs) using an extract from the aerial parts of Artemisia marschalliana Sprengel was investigated in this study. The synthesized AgNPs using A. marschalliana extract was analyzed by UV-visible spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. Characteristic absorption bands of AgNPs were found near 430 nm in the UV-vis spectrum...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27122545/mad2b-mediated-snon-downregulation-is-implicated-in-fibroblast-activation-and-tubulointerstitial-fibrosis
#14
Hui Tang, Hua Su, Di Fan, Chen Ye, Chun-Tao Lei, Hua-Jun Jiang, Pan Gao, Fang-Fang He, Chun Zhang
MAD2B, an anaphase-promoting complex/cyclosome (APC/C) inhibitor and a small subunit of DNA polymerase ζ, is indispensible for mitotic checkpoint control and DNA repair. Previously, we established that MAD2B is expressed in glomerular and tubulointerstitial compartments and participates in high glucose-induced podocyte injury. However, its role in other renal diseases remains elusive. In the present study, we aim to illustrate the potential role of MAD2B in the pathogenesis of renal fibrosis. By immunofluorescence and Western blotting, we found MAD2B expression is obviously increased in tubulointerstitial fibrosis (TIF) patients and unilateral ureteral obstruction (UUO) mice...
July 1, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27106222/discovery-of-organophosphate-resistance-related-genes-associated-with-well-known-resistance-mechanisms-of-plutella-xylostella-l-lepidoptera-plutellidae-by-rna-seq
#15
Ju-Chun Hsu, Yu-Yu Lin, Chia-Che Chang, Kuo-Hsun Hua, Mei-Ju May Chen, Li-Hsin Huang, Chien-Yu Chen
Pesticide resistance poses many challenges for pest control, particularly for destructive pests such as diamondback moths (Plutella xylostella). Organophosphates have been used in the field since the 1950s, leading to selection for resistance-related gene variants and the development of resistance to new insecticides in the diamondback moth. Identifying actual and potential genes involved in resistance could offer solutions for control. This study established resistant diamondback moth strains from two different collections using mevinphos...
April 22, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/26902951/a-novel-monolithic-controlled-delivery-system-of-resveratrol-for-enhanced-hepatoprotection-nanoformulation-development-pharmacokinetics-and-pharmacodynamics
#16
Anjali Singh, Iqbal Ahmad, Sayeed Ahmad, Zeenat Iqbal, Farhan J Ahmad
The current investigation aims to present a novel solid lipid-based nanoparticulate system of resveratrol (RV) for the effective treatment of liver cirrhosis. A simplified solvent injection method was employed and the Box-Behnken experimental design was applied for optimization to get a window particle size of 150-200 nm having maximum entrapment efficiency as well as % release. Optimized resveratrol solid lipid nanoparticles (RV-SLNs) (SR-1) of appropriate characteristics (particle size = 191.1 ± 10...
September 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/26733167/downregulation-of-cd73-in-4t1-breast-cancer-cells-through-sirna-loaded-chitosan-lactate-nanoparticles
#17
Farhad Jadidi-Niaragh, Fatemeh Atyabi, Ali Rastegari, Esmail Mollarazi, Melika Kiani, Alireza Razavi, Mehdi Yousefi, Nasim Kheshtchin, Hadi Hassannia, Jamshid Hadjati, Fazel Shokri
The immunosuppressive factors in tumor microenvironment enhance tumor growth and suppress anti-tumor immune responses. Adenosine is an important immunosuppressive factor which can be secreted by both tumor and immune cells trough action of two cell surface ecto-nucleotidase molecules CD39 and CD73. Blocking the adenosine generating molecules has emerged as an effective immunotherapeutic approach for treatment of cancer. In this study, CD73-siRNA encapsulated into chitosan-lactate (ChLa) nanoparticles (NPs) was employed to suppress the expression of CD73 molecule on 4T1 breast tumor cells, in vitro...
June 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/26724599/mat-gene-structure-and-mating-behavior-of-hymenoscyphus-fraxineus-and-hymenoscyphus-albidus
#18
Tea Wey, Markus Schlegel, Sophie Stroheker, Andrin Gross
Recently, different reproductive modes were proposed between the emerging forest pathogen Hymenoscyphus fraxineus and its closely related avirulent sister species, Hymenoscyphus albidus. In the present study, inter- and intraspecific crosses were performed to experimentally assess the reproduction barriers between the two species and to verify H. albidus' putative haploid-selfing reproductive mode. By means of H. fraxineus-specific microsatellite markers, no hybridization was observed in 29 apothecia that emerged from inter-specific crosses, suggesting reproduction barriers are well-established...
February 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/26646350/steroid-receptors-and-aromatase-gene-expression-in-different-brain-areas-of-copulating-and-sexually-sluggish-male-rats
#19
Anaid Antaramian, Adriana González-Gallardo, Carlos García-Ugalde, Wendy Portillo, Raúl G Paredes
INTRODUCTION: Sexually sluggish (SS) males have been identified in several species of mammals including rats. These animals take more than 30 minutes to ejaculate; they do not ejaculate or do so inconsistently despite being tested repeatedly with sexually receptive females. Different brain areas and hormones play an important role in the control of male sexual behavior. AIMS: Determine gene expression for the androgen receptor (AR), the estrogen receptor alpha (ERα), the progesterone receptor (PR), and the aromatase enzyme (ARO), in brain regions important in the control of male sexual behavior including the medial preoptic area (MPOA), the amygdala (AMG), the olfactory bulb (OB), and, as a control, the cortex (CTX) of copulating (C) and SS male rats...
December 2015: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/26518724/composites-of-malonic-acid-diamides-and-phospholipids-impact-of-lipoplex-stability-on-transfection-efficiency
#20
Christopher Janich, Christian Wölk, Frank Erdmann, Thomas Groth, Gerald Brezesinski, Bodo Dobner, Andreas Langner
The use of cationic lipids as gene delivery systems is a basic method in gene therapy. Through ongoing research, lipofection is currently the leader of non-viral vectors in clinical trials. However, in order to unleash the full potential of lipofection further intensive investigations are indispensable. In this study, various lipoplex formulations were compared regarding their ability to bind DNA. To obtain information about a possible premature release of DNA at the cell surface, heparin and chondroitin dependent lipoplex destabilization experiments were carried out...
December 28, 2015: Journal of Controlled Release: Official Journal of the Controlled Release Society
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