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https://www.readbyqxmd.com/read/28324642/cinnamon-reduces-inflammatory-response-in-intestinal-fibroblasts-in-vitro-and-in-colitis-in-vivo-leading-to-decreased-fibrosis
#1
Yvonne Hagenlocher, Sabrina Satzinger, Mehtap Civelek, Katharina Feilhauer, Jörg Köninger, Stephan C Bischoff, Axel Lorentz
Intestinal fibrosis, a complication of inflammatory bowel disease, is currently being addressed by surgery alone, with no adequate alternative therapy available for patients. We propose that anti-inflammatory plant substances like cinnamon extract (CE) or its main compound cinnamaldeyde (CA) could aid in therapy. We recently found CE reducing inflammation in murine colitis. Here, we analysed effects of CE on fibrosis in IL-10(-/-) colitis METHODS AND RESULTS: IL-10(-/-) and wild type (wt) mice were orally treated with/without vehicle or CE...
March 21, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28323587/metabolic-syndrome-and-periodontal-disease-among-civilian-pilots
#2
Xi Chen, Lu Xie, Yang Liu, Duanjing Chen, Qing Yu, Xueqi Gan, Haiyang Yu
BACKGROUND: Metabolic syndrome (MetS) is a cluster of metabolic abnormalities that can affect civilian pilots' health. Its prevalence and the correlation with periodontal disease (PD) among pilots had not been previously reported. The aim of the study was to determine MetS and PD status, and to reveal their relationship in a representative sample of Chinese civilian pilots. METHODS: We investigated 303 civilian pilots (mean age 34.92 ± 7.66 yr). General information as well as blood and saliva samples were collected...
December 1, 2016: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28320097/asiatic-acid-ameliorates-pulmonary-fibrosis-induced-by-bleomycin-blm-via-suppressing-pro-fibrotic-and-inflammatory-signaling-pathways
#3
Shu-Hong Dong, Yan-Wei Liu, Feng Wei, Hui-Zhen Tan, Zhi-Dong Han
Idiopathic pulmonary fibrosis is known as a life-threatening disease with high mortality and limited therapeutic strategies. In addition, the molecular mechanism by which pulmonary fibrosis developed is not fully understood. Asiatic acid (AA) is a triterpenoid, isolated from Centella asiatica, exhibiting efficient anti-inflammatory and anti-oxidative activities. In our study, we attempted to explore the effect of Asiatic acid on bleomycin (BLM)-induced pulmonary fibrosis in mice. The findings indicated that pre-treatment with Asiatic acid inhibited BLM-induced lung injury and fibrosis progression in mice...
March 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28319050/extracellular-matrix-proteomics-identifies-molecular-signature-of-symptomatic-carotid-plaques
#4
Sarah R Langley, Karin Willeit, Athanasios Didangelos, Ljubica Perisic Matic, Philipp Skroblin, Javier Barallobre-Barreiro, Mariette Lengquist, Gregor Rungger, Alexander Kapustin, Ludmilla Kedenko, Chris Molenaar, Ruifang Lu, Temo Barwari, Gonca Suna, Xiaoke Yin, Bernhard Iglseder, Bernhard Paulweber, Peter Willeit, Joseph Shalhoub, Gerard Pasterkamp, Alun H Davies, Claudia Monaco, Ulf Hedin, Catherine M Shanahan, Johann Willeit, Stefan Kiechl, Manuel Mayr
BACKGROUND: The identification of patients with high-risk atherosclerotic plaques prior to the manifestation of clinical events remains challenging. Recent findings question histology- and imaging-based definitions of the "vulnerable plaque," necessitating an improved approach for predicting onset of symptoms. METHODS: We performed a proteomics comparison of the vascular extracellular matrix and associated molecules in human carotid endarterectomy specimens from 6 symptomatic versus 6 asymptomatic patients to identify a protein signature for high-risk atherosclerotic plaques...
March 20, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28316241/investigating-the-cellular-specificity-in-tumors-of-a-surface-converting-nanoparticle-by-multimodal-imaging
#5
Francois Fay, Line Hansen, Stefanie J C G Hectors, Brenda L Sanchez-Gaytan, Yiming Zhao, Jun Tang, Jazz Munitz, Amr Alaarg, Mounia S Braza, Anita Gianella, Stuart A Aaronson, Thomas Reiner, Jørgen Kjems, Robert Langer, Freek J M Hoeben, Henk M Janssen, Claudia Calcagno, Gustav J Strijkers, Zahi A Fayad, Carlos Perez-Medina, Willem J M Mulder
Active targeting of nanoparticles through surface functionalization is a common strategy to enhance tumor delivery specificity. However, active targeting strategies tend to work against long polyethylene glycol's shielding effectiveness and associated favorable pharmacokinetics. To overcome these limitations, we developed a matrix metalloproteinase-2 sensitive surface-converting polyethylene glycol coating. This coating prevents nanoparticle-cell interaction in the bloodstream but once exposed to matrix metalloproteinase-2, i...
March 19, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28315522/human-cardiac-fibroblasts-adaptive-responses-to-controlled-combined-mechanical-strain-and-oxygen-changes-in-vitro
#6
Giovanni Stefano Ugolini, Andrea Pavesi, Marco Rasponi, Gianfranco Beniamino Fiore, Roger Kamm, Monica Soncini
Upon cardiac pathological conditions such as ischemia, microenvironmental changes instruct a series of cellular responses that trigger cardiac fibroblasts-mediated tissue adaptation and inflammation. A comprehensive model of how early environmental changes may induce cardiac fibroblasts (CF) pathological responses is far from being elucidated, partly due to the lack of approaches involving complex and simultaneous environmental stimulation. Here, we provide a first analysis of human primary CF behavior by means of a multi-stimulus microdevice for combined application of cyclic mechanical strain and controlled oxygen tension...
March 18, 2017: ELife
https://www.readbyqxmd.com/read/28315458/cyperus-rotundus-l-extract-suppresses-rankl-induced-osteoclastogenesis-through-nfatc1-c-fos-downregulation-and-prevent-bone-loss-in-ovx-induced-osteoporosis-rat
#7
Chang-Jun Kum, Eun-Young Kim, Jae-Hyun Kim, Bina Lee, Ju-Hee Min, Jun Heo, Jong-Hyun Kim, Mijung Yeom, Youngjoo Sohn, Hyuk-Sang Jung
ETHNOPHARMACOLOGICAL RELEVANCE: Cyperus Rotundus L. (CyR) has been widely used for the treatment of gynecologic disorder. Recent studies have reported that CyR can prevent the formation of cystic follicles and ovarian malfunction. However, the effects of CyR on osteoclastogenesis and postmenopausal osteoporosis remain unknown. AIM OF THE STUDY: This study was aimed to investigate the preventive effects of CyR on RANKL-induced osteoclast formation and ovariectomy (OVX)-induced bone loss...
March 15, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28315443/progesterone-modulates-diabetes-hyperglycemia-induced-changes-in-the-central-nervous-system-and-sciatic-nerve
#8
Fahim Atif, Megan C Prunty, Nefize Turan, Donald G Stein, Seema Yousuf
We investigated the effect of progesterone (P4) treatment on diabetes/hyperglycemia-induced pathological changes in brain, spinal cord and sciatic nerve tissue in male rats. Animals were rendered hyperglycemic by a single dose of streptozotocin (STZ). P4 treatment was started after hyperglycemia was confirmed and body weight and blood glucose levels were monitored once/week for 5 weeks. Rats underwent behavioral testing at week 5 and were then euthanized for histology. We assessed the expression of markers of angiogenesis (vascular endothelial growth factor (VEGF)), inflammation (interleukin-6 (IL-6)) and tissue injury (CD11b, NG2, COX2 and matrix metalloproteinase-2 (MMP-2)) in the brain, spinal cord and sciatic nerve...
March 14, 2017: Neuroscience
https://www.readbyqxmd.com/read/28315357/therapeutic-potential-of-krait-venom
#9
Antony Gomes, Partha Pratim Saha, Shamik Bhattacharya, Sourav Ghosh, Aparna Gomes
Kraits belong to Elapideae and are widely distributed in East and South-East Asian countries. Krait venom possesses neurotoxins, membrane toxins, cardiotoxins, three finger toxins, metalloproteinases, cholinesterases, L-amino acid oxidases and serine proteases. The therapeutic potential of krait venom in pathophysiological conditions such as microbial and parasitic infections, cancer, arthritis, inflammation and blood coagulation disorder is discussed in this review. More intensive new research ventures are required to establish the therapeutic potential of krait venom in complex and emerging diseases...
March 14, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28315256/simultaneous-detection-of-metalloprotease-activities-in-complex-biological-samples-using-the-prama-proteolytic-activity-matrix-assay-method
#10
Catharina Conrad, Miles A Miller, Jörg W Bartsch, Uwe Schlomann, Douglas A Lauffenburger
Proteolytic Activity Matrix Analysis (PrAMA) is a method for simultaneously determining the activities of specific Matrix Metalloproteinases (MMPs) and A Disintegrin and Metalloproteinases (ADAMs) in complex biological samples. In mixtures of unknown proteases, PrAMA infers selective metalloproteinase activities by using a panel of moderately specific FRET-based polypeptide protease substrates in parallel, typically monitored by a plate-reader in a 96-well format. Fluorescence measurements are then quantitatively compared to a standard table of catalytic efficiencies measured from purified mixtures of individual metalloproteinases and FRET substrates...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28314762/cerebral-microhemorrhages-mechanisms-consequences-and-prevention
#11
Zoltan I Ungvari, Stefano Tarantini, Angelia C Kirkpatrick, Anna Csiszar, Calin I Prodan
The increasing prevalence of multifocal cerebral microhemorrhages (CMHs, also known as "cerebral microbleeds") is a significant, newly recognized problem in the aging population of the Western world. CMHs are associated with rupture of small intracerebral vessels and are thought to progressively impair neuronal function, potentially contributing to cognitive decline, geriatric psychiatric syndromes and gait disorders. Clinical studies show that aging and hypertension significantly increase prevalence of CMHs...
March 17, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28314761/hypoxia-mediated-regulation-of-the-secretory-properties-of-mitral-valve-interstitial-cells
#12
Kareem Salhiyyah, Padmini Sarathchandra, Najma Latif, Magdi H Yacoub, Adrian H Chester
The sophisticated function of the mitral valve depends to a large extent on the extracellular matrix (ECM) and specific cellular components. These are tightly regulated by a repertoire of mechanical stimuli and biological pathways. One potentially important pathway is hypoxia. The purpose of this investigation is to determine the effect of hypoxia on the regulation of mitral valve interstitial cells (MVIC) with respect to the synthesis and secretion of extracellular matrix proteins. Hypoxia resulted in reduced production of total collagen and sulphated glycosaminoglycans (sGAG) in cultured porcine MVIC...
March 17, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28314476/polydatin-alleviates-alcohol-induced-acute-liver-injury-in-mice-relevance-of-matrix-metalloproteinases-mmps-and-hepatic-antioxidants
#13
Meghana Koneru, Bidya Dhar Sahu, Sagarika Gudem, Madhusudana Kuncha, Halley Gora Ravuri, Jerald Mahesh Kumar, Eswar Kumar Kilari, Ramakrishna Sistla
BACKGROUND: Alcohol, a most commonly consumed beverage, is the foremost cause of liver injury throughout the world. Polydatin, a stilbenoid glucoside, was known to possess antioxidant and anti-inflammatory properties and is being investigated for use in various disorders. PURPOSE: The present study was intended at investigating the hepatoprotective efficacy of polydatin against acute-alcohol induced liver injury model in mice. STUDY DESIGN: C57BL/6 mice were fed with five doses of 50% ethyl alcohol (10ml/kg body weight) to induce acute liver injury...
April 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28306533/ros-mediated-induction-of-apoptosis-by-benzoquinone-embelin-in-human-colon-adenocarcinoma-cells-ht-29
#14
Kodandaram Sumalatha, Mohan Gowda, Sreepriya Meenakshisundaram
Background Embelin is a benzoquinone reported to possess anticancer activity in several in vivo and in vitro models of carcinogenesis, especially hematopoietic and prostate malignancy. A detailed investigation on the influence of embelin on epithelial malignancy model system, especially colon adenocarcinoma, is lacking. The objective of the current study is to investigate the antiproliferative, antiinvasive and proapoptotic potential of embelin on colon adenocarcinoma cell line HT-29. Methods The effect of embelin (35 µg/mL for 24 h) on cell proliferation was assessed by Sulforhodamine B assay and bromodeoxyuridine incorporation test, antiinvasive effect by Boyden chamber assay and scratch assay...
March 17, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28304250/tranexamic-acid-and-supportive-measures-to-treat-wasting-marmoset-syndrome
#15
Takuro Yoshimoto, Kimie Niimi, Eiki Takahashi
Wasting marmoset syndrome (WMS) has high incidence and mortality rates and is one of the most important problems in captive common marmoset (Callithrix jacchus) colonies. Despite several reports on WMS, little information is available regarding its reliable treatment. We previously reported that marmosets with WMS had high serum levels of matrix metalloproteinase 9 (MMP9). MMP9 is thought to be a key enzyme in the pathogenesis of inflammatory bowel disease, the main disease state of WMS, and is activated by plasmin, a fibrinolytic factor...
December 1, 2016: Comparative Medicine
https://www.readbyqxmd.com/read/28304138/rapid-and-efficient-one-step-purification-of-a-serralysin-family-protease-by-using-a-p-aminobenzamidine-modified-affinity-medium
#16
Shangyong Li, Linna Wang, Shengxiang Lin, Juan Yang, Zibin Ma, Yuejun Wang, Junzhong Liu, Jianhua Hao, Mi Sun
The metalloproteinase MP belongs to the serralysin family, which is involved in important functions such as nutrient acquisition and infection pathogenesis. Serralysin proteases in highly purified form are commonly used at the industrial level with several purposes. In this study, we set up an efficient and rapid purification protocol for MP using a p-aminobenzamidine-modified affinity chromatography. The affinity medium was synthesized by using p-aminobenzamidine as affinity ligand immobilized via cyanuric chloride spacer to Sepharose 6B sorbent carrier...
March 17, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28303501/isoflurane-postconditioning-inhibits-tpa-induced-matrix-metalloproteinases-activation-after-hypoxic-injury-via-low-density-lipoprotein-receptor-related-protein-and-extracellular-signal-regulated-kinase-pathway
#17
So Yeon Kim, So Yeong Cheon, Eun Jung Kim, Jae Hoon Lee, Eun Hee Kam, Jeong Min Kim, Miran Park, Bon-Nyeo Koo
Tissue plasminogen activator (tPA) is the only recommended pharmacological treatment for acute ischemic stroke. However, tPA can induce intracerebral hemorrhage by blood-brain barrier breakdown through an increase in matrix metalloproteinases (MMPs). Previously, we showed that isoflurane postconditioning reduced intracranial hemorrhage following tPA treatment after cerebral ischemia. Here, we investigated the mechanism by which isoflurane postconditioning reduces tPA-induced MMP-2 and MMP-9 activation following hypoxia/reoxygenation (H/R) in brain endothelial cells...
March 16, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28302318/regional-differences-of-tibial-and-femoral-cartilage-in-the-chondrocyte-gene-expression-immunhistochemistry-and-composite-in-different-stages-of-osteoarthritis
#18
A Lahm, D Dabravolski, H Spank, H Merk, J Esser, R Kasch
The function of articular cartilage as an avascular tissue is mainly served by collagen type II and proteoglycan molecules. Within this matrix homeostasis between production and breakdown of the matrix is exceptionally sensitive. The current study was conducted to identify regional differences in specific alterations in cartilage composition during the osteoarthritic process of the human knee joint. Therefor the changes in the expression of the key molecules of the extracellular matrix were measured in dependence of the anatomical side (femoral vs tibial) and associated with immunohistochemistry and quantitative measurement...
February 24, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28302163/matrix-metalloproteinase-activity-stimulates-n-cadherin-shedding-and-the-soluble-n-cadherin-ectodomain-promotes-classical-microglial-activation
#19
Katherine Conant, Stefano Daniele, P Lorenzo Bozzelli, Tsion Abdi, Amanda Edwards, Arek Szklarczyk, India Olchefske, David Ottenheimer, Kathleen Maguire-Zeiss
BACKGROUND: Matrix metalloproteinases (MMPs) are a family of enzymes that are typically released from intracellular stores to act on specific extracellular substrates. MMP expression and activity can be increased in a neuronal activity-dependent manner, and further increased in response to tissue injury. MMP substrates include cell adhesion molecules (CAMs) that are abundantly expressed in the brain and well positioned for membrane proximal cleavage. Importantly, CAM integrity is important to synaptic structure and axon-myelin interactions, and shed ectodomains may themselves influence cellular function...
March 17, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28301987/properties-of-epithelial-cells-and-vaginal-secretions-in-pregnant-women-when-lactobacillus-crispatus-or-lactobacillus-iners-dominate-the-vaginal-microbiome
#20
Julie Leizer, Dimitrios Nasioudis, Larry J Forney, G Maria Schneider, Karol Gliniewicz, Allison Boester, Steven S Witkin
OBJECTIVE: Our objective was to determine differences in properties of vaginal epithelial cells and the composition of vaginal secretions when Lactobacillus crispatus or Lactobacillus iners are numerically dominant in the vaginal microenvironment of pregnant women. METHODS: The vaginal microbiomes of 157 first-trimester pregnant women were identified by classifying partial 16S gene sequences amplified from the V1 to V3 region of bacterial ribosomal 16S RNA genes...
January 1, 2017: Reproductive Sciences
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