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https://www.readbyqxmd.com/read/28737701/application-of-extrusion-based-hydrogel-bioprinting-for-cartilage-tissue-engineering
#1
REVIEW
Fu You, B Frank Eames, Xiongbiao Chen
Extrusion-based bioprinting (EBB) is a rapidly developing technique that has made substantial progress in the fabrication of constructs for cartilage tissue engineering (CTE) over the past decade. With this technique, cell-laden hydrogels or bio-inks have been extruded onto printing stages, layer-by-layer, to form three-dimensional (3D) constructs with varying sizes, shapes, and resolutions. This paper reviews the cell sources and hydrogels that can be used for bio-ink formulations in CTE application. Additionally, this paper discusses the important properties of bio-inks to be applied in the EBB technique, including biocompatibility, printability, as well as mechanical properties...
July 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28734756/3d-bioprinting-for-reconstructive-surgery-principles-applications-and-challenges
#2
REVIEW
Zita M Jessop, Ayesha Al-Sabah, Matthew D Gardiner, Emman Combellack, Karl Hawkins, Iain S Whitaker
Despite the increasing laboratory research in the growing field of 3D bioprinting, there are few reports of successful translation into surgical practice. This review outlines the principles of 3D bioprinting including software and hardware processes, biocompatible technological platforms and suitable bioinks. The advantages of 3D bioprinting over traditional tissue engineering techniques in assembling cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissue macro-, micro- and nanoarchitectures are discussed, together with an overview of current progress in bioprinting tissue types relevant for plastic and reconstructive surgery...
June 9, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28731220/mechanically-tunable-bioink-for-3d-bioprinting-of-human-cells
#3
Aurelien Forget, Andreas Blaeser, Florian Miessmer, Marius Köpf, Daniela F Duarte Campos, Nicolas H Voelcker, Anton Blencowe, Horst Fischer, V Prasad Shastri
This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels can be tuned over several orders of magnitudes (5-230 Pa) while ensuring almost no change to the shear viscosity (10-17 mPa) of the bioink solution; thus enabling the fabrication of 3D structures made of different mechanical domains under identical printing parameters and low nozzle shear stress...
July 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28726587/repair-of-tympanic-membrane-perforations-with-customized-bioprinted-ear-grafts-using-chinchilla-models
#4
Che-Ying Kuo, Emmanuel Wilson, Andrew Fuson, Nidhi Gandhi, Reza Monfaredi, Audrey Jenkins, Maria Romero, Marco Santoro, John P Fisher, Kevin Cleary, Brian Reilly
The goal of this work is to develop an innovative method that combines bioprinting and endoscopic imaging to repair tympanic membrane perforations (TMPs). TMPs are a serious health issue because they can lead to both conductive hearing loss and repeated otitis media. TMPs occur in 3 to 5% of cases after ear tube placement as well as in cases of acute otitis media (the second most common infection in pediatrics), chronic otitis media with or without cholesteatoma, or as a result of barotrauma to the ear. About 55,000 tympanoplasties, the surgery performed to reconstruct TMPs, are performed every year and the commonly used cartilage grafting technique has a success rate between 43% to 100%...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726575/bioprinting-pattern-dependent-electrical-mechanical-behavior-of-cardiac-alginate-implants-characterization-and-ex-vivo-phase-contrast-microtomography-assessment
#5
Mohammad Izadifar, Paul Babyn, Michael E Kelly, Dean Chapman, Xiongbiao Chen
Three-dimensional (3D)-bioprinting techniques may be used to modulate electrical/mechanical properties and porosity of hydrogel constructs for fabrication of suitable cardiac implants. Notably, characterization of these properties after implantation remains a challenge, raising the need for the development of novel quantitative imaging techniques for monitoring hydrogel implant behavior in-situ. This study aims to (i) assess the influence of hydrogel bioprinting patterns on electrical/mechanical behavior of cardiac implants based on a 3D-printing technique and (ii) investigate the potential of synchrotron X-ray phase contrast computed tomography (PCI-CT) for estimating elastic modulus/impedance/porosity and microstructural features of 3D-printed cardiac implants in-situ via an ex-vivo study...
July 20, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28724980/handheld-co-axial-bioprinting-application-to-in-situ-surgical-cartilage-repair
#6
Serena Duchi, Carmine Onofrillo, Cathal D O'Connell, Romane Blanchard, Cheryl Augustine, Anita F Quigley, Robert M I Kapsa, Peter Pivonka, Gordon Wallace, Claudia Di Bella, Peter F M Choong
Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully addressed to generate a structure stiff enough to withstand the forces phisiologically applied on articular cartilage, while ensuring adequate cell survival for functional chondral repair...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28721949/rising-to-the-challenge-applying-biofabrication-approaches-for-better-drug-and-chemical-product-development
#7
Anthony M Holmes, Alex Charlton, Brian Derby, Lorna Ewart, Andrew Scott, Wenmiao Shu
Many industrial sectors, from pharmaceuticals to consumer products, are required to provide data on their products to demonstrate their efficacy and that they are safe for patients, consumers and the environment. This period of testing typically requires the use of animal models, the validity of which has been called into question due to the high rates of attrition across many industries. There is increasing recognition of the limitations of animal models and demands for safety and efficacy testing paradigms which embrace the latest technological advances and knowledge of human biology...
July 19, 2017: Biofabrication
https://www.readbyqxmd.com/read/28715377/creation-of-cardiac-tissue-exhibiting-mechanical-integration-of-spheroids-using-3d-bioprinting
#8
Chin Siang Ong, Takuma Fukunishi, Andrew Nashed, Adriana Blazeski, Huaitao Zhang, Samantha Hardy, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon Tomaselli, Narutoshi Hibino
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates. Within 3 days, beating spheroids form. These spheroids are then picked up by a nozzle using vacuum suction and assembled on a needle array using a 3D bioprinter. The spheroids are then allowed to fuse on the needle array...
July 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28711892/3-d-bioprinting-law-regulation-perspectives
#9
Vitalii Pashkov, Andrii Harkusha
INTRODUCTION: Achieved level of technical progress moves us closer and closer to practical use of 3-d bioprinting technologies in real life. Such perspective raise a wide variety of crucial legal issues from the acceptable model of regulation of the science and its' societal effects to problems of the commercialization of the technology and potential restrictions of its use. Some key points on concept of legal regulation of abovementioned sphere is a base of this study. MATERIAL AND METHODS: Scientific discussion on 3-D bioprinting, European Union`s and US experience in patenting of 3-D bioprinting technologies, European Medicine Agency (EMA) or the US Food and Drug Administration (FDA) regulations, European Medical Technology Industry Association (EUCOMED) Acts...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28707625/bioprinting-of-functional-vascularized-mouse-thyroid-gland-construct
#10
Elena A Bulanova, Elizaveta V Koudan, Jonathan Degosserie, Charlotte Heymans, Frederico DAS Pereira, Vladislav A Parfenov, Yi Sun, Qi Wang, Suraya A Akhmedova, Irina K Sviridova, Natalia S Sergeeva, Georgy A Frank, Yusef D Khesuani, Christophe E Pierreux, Vladimir A Mironov
Bioprinting can be defined as additive biofabrication of 3D tissues and organ constructs using tissue spheroids, capable of self-assembly, as building blocks. Thyroid gland, a relatively simple endocrine organ, is suitable for testing the proposed bioprinting technology. Here we report the bioprinting of functional vascularized mouse thyroid gland construct from embryonic tissue spheroids as a proof of concept. Based on the self-assembly principle, we generated thyroid tissue starting from thyroid spheroids (TS) and allantoic spheroids (AS), as a source of thyrocytes and endothelial cells (EC), respectively...
July 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28697953/3d-in-vitro-models-of-liver-fibrosis
#11
Leo A van Grunsven
Animal testing is still the most popular preclinical assesment model for liver fibrosis. To develop efficient anti-fibrotic therapies, robust and representative in vitro models are urgently needed. The most widely used in vitro fibrosis model is the culture-induced activation of primary rodent hepatic stellate cells. While these cultures have contributed greatly to the current understanding of hepatic stellate cell activation, they seem to be inadequate to cover the complexity of this regenerative response...
July 8, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/28677597/correlating-rheological-properties-and-printability-of-collagen-bioinks-the-effects-of-riboflavin-photocrosslinking-and-ph
#12
Nicole Diamantides, Louis Wang, Tylar Pruiksma, Joseph Siemiatkoski, Caroline Dugopolski, Sonya Shortkroff, Stephen Kennedy, Lawrence J Bonassar
Collagen has shown promise as a bioink for extrusion-based bioprinting, but further development of new collagen bioink formulations is necessary to improve their printability. Screening these formulations by measuring print accuracy is a costly and time consuming process. We hypothesized that rheological properties of the bioink before, during, and/or after gelation can be used to predict printability. In this study, we investigated the effects of riboflavin photocrosslinking and pH on type I collagen bioink rheology before, during, and after gelation and directly correlated these findings to the printability of each bioink formulation...
July 5, 2017: Biofabrication
https://www.readbyqxmd.com/read/28676704/biomaterial-free-three-dimensional-bioprinting-of-cardiac-tissue-using-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#13
Chin Siang Ong, Takuma Fukunishi, Huaitao Zhang, Chen Yu Huang, Andrew Nashed, Adriana Blazeski, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon F Tomaselli, Narutoshi Hibino
We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28676662/directing-the-self-assembly-of-tumour-spheroids-by-bioprinting-cellular-heterogeneous-models-within-alginate-gelatin-hydrogels
#14
Tao Jiang, Jose G Munguia-Lopez, Salvador Flores-Torres, Joel Grant, Sanahan Vijayakumar, Antonio De Leon-Rodriguez, Joseph M Kinsella
Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28675678/3d-bioprinting-and-the-current-applications-in-tissue-engineering
#15
REVIEW
Ying Huang, Xiao-Fei Zhang, Guifang Gao, Tomo Yonezawa, Xiaofeng Cui
Bioprinting as an enabling technology for tissue engineering possesses the promises to fabricate highly mimicked tissue or organs with digital control. As one of the biofabrication approaches, bioprinting has the advantages of high throughput and precise control of both scaffold and cells. Therefore, this technology is not only ideal for translational medicine but also for basic research applications. Bioprinting has already been widely applied to construct functional tissues such as vasculature, muscle, cartilage, and bone...
July 4, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28670447/short-term-hypoxic-preconditioning-promotes-prevascularization-in-3d-bioprinted-bone-constructs-with-stromal-vascular-fraction-derived-cells
#16
Mitchell A Kuss, Robert Harms, Shaohua Wu, Ying Wang, Jason B Untrauer, Mark A Carlson, Bin Duan
Reconstruction of complex, craniofacial bone defects often requires autogenous vascularized bone grafts, and still remains a challenge today. In order to address this issue, we isolated the stromal vascular fraction (SVF) from adipose tissues and maintained the phenotypes and the growth of endothelial lineage cells within SVF derived cells (SVFC) by incorporating an endothelial cell medium. We 3D bioprinted SVFC within our hydrogel bioinks and conditioned the constructs in either normoxia or hypoxia. We found that short-term hypoxic conditioning promoted vascularization-related gene expression, whereas long-term hypoxia impaired cell viability and vascularization...
June 5, 2017: RSC Advances
https://www.readbyqxmd.com/read/28670353/effects-of-living-cells-on-the-bioink-printability-during-laser-printing
#17
Zhengyi Zhang, Changxue Xu, Ruitong Xiong, Douglas B Chrisey, Yong Huang
Laser-induced forward transfer has been a promising orifice-free bioprinting technique for the direct writing of three-dimensional cellular constructs from cell-laden bioinks. In order to optimize the printing performance, the effects of living cells on the bioink printability must be carefully investigated in terms of the ability to generate well-defined jets during the jet/droplet formation process as well as well-defined printed droplets on a receiving substrate during the jet/droplet deposition process...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28669949/-three-dimensional-bioprinted-microstructure-promotes-proliferation-and-viability-of-murine-epithelial-stem-cells-in-vitro
#18
Yu-Fan Liu, Sha Huang, Bin Yao, Zhao Li, Xiang Li, Xiao-Bing Fu, Xu Wu
OBJECTIVE: To evaluate the effect of different microstructures prepared by three-dimensional (3D) bioprinting on proliferation and viability of the murine epithelial stem cells in vitro. METHODS: 3D cell-laden microstructures were constructed using 3 different printing nozzles with diameters of 210, 340, and 420 µm. Fluorescence microscopy and the live/dead assay kit were used to observe the proliferation and viability of the murine epithelial stem cells in the microstructures...
June 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28665236/formation-of-adipose-stromal-vascular-fraction-cell-laden-spheroids-using-a-3d-bioprinter-and-superhydrophobic-surfaces
#19
Brian Gettler, Joseph Zakhari, Piyani Gandhi, Stuart K Williams
The therapeutic infusion of adipose-derived stromal vascular fraction cells (SVF) for the treatment of multiple diseases, has progressed to numerous human clinical trials; however, the often poor retention of the cells following implantation remains a common drawback of direct cell injection. One solution to cellular retention at the injection site has been the use of biogels to encapsulate cells within a microenvironment prior to and upon implantation. The current study utilized 3D bioprinting technology to evaluate the ability to form SVF laden spheroids with collagen I as a gel forming biomatrix...
June 30, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28662187/scientometric-and-patentometric-analyses-to-determine-the-knowledge-landscape-in-innovative-technologies-the-case-of-3d-bioprinting
#20
Marisela Rodríguez-Salvador, Rosa María Rio-Belver, Gaizka Garechana-Anacabe
This research proposes an innovative data model to determine the landscape of emerging technologies. It is based on a competitive technology intelligence methodology that incorporates the assessment of scientific publications and patent analysis production, and is further supported by experts' feedback. It enables the definition of the growth rate of scientific and technological output in terms of the top countries, institutions and journals producing knowledge within the field as well as the identification of main areas of research and development by analyzing the International Patent Classification codes including keyword clusterization and co-occurrence of patent assignees and patent codes...
2017: PloS One
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