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Long Zhang, Fan Zhang, Xi Yang, Kai Leng, Yi Huang, Yongsheng Chen
Supercapacitors based on activated carbon prepared from various pollens show good specific capacitance (207 F g(-1) ) and large gravimetric and volumetric energy density (∼88 Wh kg(-1) and ∼44 Wh L(-1) , respectively) in ionic liquid electrolytes, which are much better than those of RP20 (commercial activated carbon) or graphene-based supercapacitors.
April 22, 2013: Small
Paramasivan Vijayapalani, Jeff Chien-Fu Chen, Ming-Ru Liou, Hsin-Chuan Chen, Yau-Heiu Hsu, Na-Sheng Lin
Bamboo mosaic virus (BaMV) satellite RNA (satBaMV) depends on BaMV for its replication and encapsidation. SatBaMV-encoded P20 protein is an RNA-binding protein that facilitates satBaMV systemic movement in co-infected plants. Here, we examined phosphorylation of P20 and its regulatory functions. Recombinant P20 (rP20) was phosphorylated by host cellular kinase(s) in vitro, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and mutational analyses revealed Ser-11 as the phosphorylation site...
January 2012: Nucleic Acids Research
Weerawan Sithigorngul, Sombat Rukpratanporn, Nusara Sittidilokratna, Nilawan Pecharaburanin, Siwaporn Longyant, Parin Chaivisuthangkura, Paisarn Sithigorngul
A simple yellow head virus (YHV) "strip test" was developed using monoclonal antibody Y19 (against the p20 structural protein) conjugated with colloidal gold as the detector antibody. Rabbit anti-recombinant p20 (rp20) protein antibody was used as a capture antibody at the test line (T) and goat anti-mouse IgG antibody (GAM) was used as the capture antibody at the control line (C). The ready-to-use strip was housed in a plastic case for convenient application and stored in the desiccated plastic bag. A sample volume of 100 microl of either haemolymph or gill or appendage homogenates in application buffer was applied to the sample chamber at one end of the strip and allowed to flow by chromatography through the nitrocellulose membrane to the other end...
March 2007: Journal of Virological Methods
A S De Zoysa, A Efstratiou
The usefulness of random amplification of polymorphic DNA (RAPD) analysis with Ready-To-Go RAPD beads was investigated for the rapid differentiation of Corynebacterium diphtheriae isolates from Eastern Europe and neighbouring countries. A selection of 45 C. diphtheriae isolates of known origin, biotype, toxigenicity status and ribotype were examined by RAPD. Twenty RAPD profiles (designated Rp1-Rp20) were revealed among the 45 isolates. There was 100% correlation between RAPD profiles and ribotypes. Preliminary studies showed that the use of crude DNA preparations resulted in poor amplification and the patterns were not reproducible...
April 1999: Journal of Medical Microbiology
R L Tobia, R J Fischer
BACKGROUND: A prototype demand-flow medical ventilator for intensive care unit (ICU) applications has been developed with the ability to maintain continuous pressure regulation of proximal airway pressure during both inspiratory and expiratory respiratory phases. The performance of this system was investigated in laboratory tests of continuous positive airway pressure (CPAP) mode, a ventilatory mode in which airway pressure regulation is strongly challenged. MATERIALS & METHODS: Comparative tests of the pressure-regulating ventilator (PRV) prototype and a Puritan-Bennett 7200a (PB7200a) ventilator were made in three performance categories: pressure-volume product error, peak pressure error during inspiration, and peak pressure error during expiration...
March 1994: Respiratory Care
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