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https://www.readbyqxmd.com/read/28874376/genomics-and-susceptibility-profiles-of-extensively-drug-resistant-xdr-pseudomonas-aeruginosa-from-spain
#1
Ester Del Barrio-Tofiño, Carla López-Causapé, Gabriel Cabot, Alba Rivera, Natividad Benito, Concepción Segura, María Milagro Montero, Luisa Sorlí, Fe Tubau, Silvia Gómez-Zorrilla, Nuria Tormo, Raquel Durá-Navarro, Esther Viedma, Elena Resino-Foz, Marta Fernández-Martínez, Claudia González-Rico, Izaskun Alejo-Cancho, Jose Antonio Martínez, Cristina Labayru-Echverria, Carlos Dueñas, Ignacio Ayestarán, Laura Zamorano, Luis Martinez-Martinez, Juan Pablo Horcajada, Antonio Oliver
This study assessed the molecular epidemiology, resistance mechanisms and susceptibility profiles of a collection of 150 XDR P. aeruginosa clinical isolates obtained from a 2015 Spanish multicenter study, with a particular focus on resistome analysis in relation to ceftolozane/tazobactam susceptibility. Broth microdilution MICs revealed that nearly all (>95%) of the isolates were nonsusceptible to piperacillin/tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem and ciprofloxacin. Most of them were also resistant to tobramycin (77%), whereas nonsusceptibility rates were lower for ceftolozane/tazobactam (31%), amikacin (7%) and colistin (2%)...
September 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28865758/high-doses-of-systemic-corticosteroids-in-patients-hospitalised-for-exacerbation-of-chronic-obstructive-pulmonary-disease-a-cohort-study
#2
J A Rueda-Camino, D Bernal-Bello, J Canora-Lebrato, L Velázquez-Ríos, V García de Viedma-García, M Guerrero-Santillán, M A Duarte-Millán, R Cristóbal-Bilbao, A Zapatero-Gaviria
OBJECTIVES: To assess the effect of high doses of corticosteroids in patients hospitalised for exacerbation of chronic obstructive pulmonary disease (COPD). PATIENTS AND METHODS: A prospective cohort study was conducted on patients hospitalized with COPD between January and March 2015, grouped according to the glucocorticoid dosage administered (cutoff, 40mg of prednisone/day). We compared the results of hospital stay, readmission and mortality at 3 months of discharge...
August 30, 2017: Revista Clínica Española
https://www.readbyqxmd.com/read/28721087/epicrania-fugax-combining-forward-and-backward-paroxysms-in-the-same-patient-the-first-four-cases
#3
Johanna Barón-Sánchez, Álvaro Gutiérrez-Viedma, Marina Ruiz-Piñero, Alicia Pérez-Pérez, Ángel Luis Guerrero, María L Cuadrado
BACKGROUND: The first description of epicrania fugax (EF) reported brief painful paroxysms that start in posterior regions of the scalp and move forward to reach the ipsilateral forehead, eye, or nose. A backward variation, wherein pain stems from frontal areas and radiates to the posterior scalp, has also been acknowledged. We report four patients with features reminiscent of EF and the coexistence of forward and backward pain paroxysms. METHODS: We considered all patients attending the headache outpatient office at two tertiary hospitals from March 2008 to March 2016...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28527287/-identification-of-the-mycobacterium-tuberculosis-beijing-lineage-in-ecuador
#4
Patricia Jiménez, Karina Calvopiña, Diana Herrera, Carlos Rojas, Laura Pérez-Lago, Marcelo Grijalva, Remedios Guna, Darío García-de Viedma
INTRODUCTION: Mycobacterium tuberculosis Beijing lineage isolates are considered to be especially virulent, transmissible and prone to acquire resistances. Beijing strains have been reported worldwide, but studies in Latin America are still scarce. The only multinational study performed in the region indicated a heterogeneous distribution for this lineage, which was absent in Chile, Colombia and Ecuador, although further studies found the lineage in Chile and Colombia. OBJECTIVE: To search for the presence of the Beijing lineage in Ecuador, the only country in the region where it remains unreported...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28114806/infratrochlear-neuralgia-a-prospective-series-of-seven-patients-treated-with-infratrochlear-nerve-blocks
#5
Rocío-Nur Villar-Quiles, Héctor García-Moreno, Diego Mayo, Álvaro Gutiérrez-Viedma, María-Isabel Ramos, Javier Casas-Limón, María-Luz Cuadrado
Background Infratrochlear neuralgia is a recently described painful cranial neuropathy that causes pain in the internal angle of the orbit and the medial upper eyelid, the upper bridge of the nose and/or the lacrimal caruncle. We aim to present seven new cases of infratrochlear neuralgia treated with anaesthetic nerve blocks. Methods Over an 18-month period, we prospectively identified seven cases of infratrochlear neuralgia among the patients attending the Headache Unit in a tertiary hospital. Anaesthetic blocks were performed by injecting 0...
January 1, 2017: Cephalalgia: An International Journal of Headache
https://www.readbyqxmd.com/read/27861819/lacrimal-nerve-blocks-for-three-new-cases-of-lacrimal-neuralgia
#6
María-Luz Cuadrado, Álvaro Gutiérrez-Viedma, Lorenzo Silva-Hernández, Aida Orviz, Héctor García-Moreno
OBJECTIVE: Our aim was to report three new cases of lacrimal neuralgia and their response to superficial nerve blocks. BACKGROUND: Lacrimal neuralgia has been recently described as a pain in the territory supplied by the lacrimal nerve, at the lateral upper eyelid and/or the adjacent area of the temple. The pain is typically accompanied by tenderness on palpation of the lacrimal nerve at the superoexternal angle of the orbit. METHODS: Between January 2015 and June 2016, we prospective identified three cases of lacrimal neuralgia among the patients attending the Headache Unit in a tertiary hospital...
March 2017: Headache
https://www.readbyqxmd.com/read/27682128/urgent-implementation-in-a-hospital-setting-of-a-strategy-to-rule-out-secondary-cases-caused-by-imported-extensively-drug-resistant-mycobacterium-tuberculosis-strains-at-diagnosis
#7
Laura Pérez-Lago, Miguel Martínez-Lirola, Sergio García, Marta Herranz, Igor Mokrousov, Iñaki Comas, Llúcia Martínez-Priego, Emilio Bouza, Darío García-de-Viedma
Current migratory movements require new strategies for rapidly tracking the transmission of high-risk imported Mycobacterium tuberculosis strains. Whole-genome sequencing (WGS) enables us to identify single-nucleotide polymorphisms (SNPs) and therefore design PCRs to track specific relevant strains. However, fast implementation of these strategies in the hospital setting is difficult because professionals working in diagnostics, molecular epidemiology, and genomics are generally at separate institutions. In this study, we describe the urgent implementation of a system that integrates genomics and molecular tools in a genuine high-risk epidemiological alert involving 2 independent importations of extensively drug resistant (XDR) and pre-XDR Beijing M...
December 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27432624/living-with-cluster-headache-a-qualitative-study-of-patients-perspectives
#8
Domingo Palacios-Ceña, Blanca Talavera, Pedro López-Ruiz, Álvaro Gutiérrez-Viedma, María Palacios-Ceña, José A Arias, César Fernández-de-Las-Peñas, María L Cuadrado
OBJECTIVE: Our aim was to explore the views and experiences of a group of Spanish men suffering from cluster headache (CH). BACKGROUND: CH has considerable effects on patients' quality of life, impairs everyday activities, and can modify lifestyle. This is the first time the experience of patients with CH is examined in a clinical study using a qualitative, phenomenological approach. METHODS: We conducted a qualitative phenomenological study exploring how 20 male patients with CH, followed at the Headache Unit of a Spanish hospital, perceived their disease...
July 2016: Headache
https://www.readbyqxmd.com/read/27288355/greater-occipital-nerve-block-for-the-acute-treatment-of-prolonged-or-persistent-migraine-aura
#9
María L Cuadrado, Ángel Aledo-Serrano, Pedro López-Ruiz, Álvaro Gutiérrez-Viedma, Cristina Fernández, Aida Orviz, José A Arias
Background Presently, there is no evidence to guide the acute treatment of migraine aura. We aimed to describe the effect of greater occipital nerve (GON) anaesthetic block as a symptomatic treatment for long-lasting (prolonged or persistent) migraine aura. Methods Patients who presented with migraine aura lasting > 2 hours were consecutively recruited during one year at the Headache Unit and the Emergency Department of a tertiary hospital. All patients underwent a bilateral GON block with bupivacaine 0...
July 2017: Cephalalgia: An International Journal of Headache
https://www.readbyqxmd.com/read/27273384/pain-paroxysms-with-coronal-radiation-case-series-and-proposal-of-a-new-variant-of-epicrania-fugax
#10
MULTICENTER STUDY
Javier Casas-Limón, María L Cuadrado, Marina Ruiz, Elena Martínez, Álvaro Gutiérrez-Viedma, Carlos M Ordás, Ángel L Guerrero
OBJECTIVE: We aim to report 4 patients with brief pain paroxysms whose clinical features remind of typical epicrania fugax (EF), except for the direction along a transverse trajectory. BACKGROUND: EF has been defined as a brief paroxysmal head pain, with stabbing quality, describing a linear or zig-zag trajectory across the surface of one hemicranium. METHODS: We considered all patients attending the headache outpatient office at 3 tertiary hospitals from March 2008 to December 2015...
June 2016: Headache
https://www.readbyqxmd.com/read/27157988/differences-between-a-probable-and-proven-bcg-infection-following-intravesical-instillations-16-years-experience-in-a-tertiary-care-hospital
#11
Marcela González-Del Vecchio, María Jesús Ruíz-Serrano, Paloma Gijón, Mar Sánchez-Somolinos, Viviana de Egea, Darío García de Viedma, María Norberta Sánchez Fresneda, Emilio Bouza
BACKGROUND: Intravesical instillation of Bacillus Calmette-Guérin (BCG) is an efficient immunotherapy for superficial bladder cancer. BCG infection represents a major yet uncommon adverse event that occurs in 5-10% of the patients treated with BCG instillations, though the pathogenesis of this entity is not clear. METHODS: We report two cases of patients presented at our institution with BCG infection after instillation: one with microbiological BCG isolate and another without, and review all the medical records of patients instilled with BCG in our institution from 1996 until 2012, comparing patients with probable and proven BCG infection...
July 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27033428/a-scoring-system-to-determine-risk-of-delayed-bleeding-after-endoscopic-mucosal-resection-of-large-colorectal-lesions
#12
MULTICENTER STUDY
Eduardo Albéniz, María Fraile, Berta Ibáñez, Pedro Alonso-Aguirre, David Martínez-Ares, Santiago Soto, Carla Jerusalén Gargallo, Felipe Ramos Zabala, Marco Antonio Álvarez, Joaquín Rodríguez-Sánchez, Fernando Múgica, Óscar Nogales, Alberto Herreros de Tejada, Eduardo Redondo, Carlos Guarner-Argente, Noel Pin, Helena León-Brito, Remedios Pardeiro, Leopoldo López-Roses, Manuel Rodríguez-Téllez, Alejandra Jiménez, Felipe Martínez-Alcalá, Orlando García, Joaquín de la Peña, Akiko Ono, Fernando Alberca de Las Parras, María Pellisé, Liseth Rivero, Esteban Saperas, Francisco Pérez-Roldán, Antonio Pueyo Royo, Javier Eguaras Ros, Alba Zúñiga Ripa, Mar Concepción-Martín, Patricia Huelin-Álvarez, Juan Colán-Hernández, Joaquín Cubiella, David Remedios, Xavier Bessa I Caserras, Bartolomé López-Viedma, Julyssa Cobian, Mariano González-Haba, José Santiago, Juan Gabriel Martínez-Cara, Eduardo Valdivielso
BACKGROUND & AIMS: After endoscopic mucosal resection (EMR) of colorectal lesions, delayed bleeding is the most common serious complication, but there are no guidelines for its prevention. We aimed to identify risk factors associated with delayed bleeding that required medical attention after discharge until day 15 and develop a scoring system to identify patients at risk. METHODS: We performed a prospective study of 1214 consecutive patients with nonpedunculated colorectal lesions 20 mm or larger treated by EMR (n = 1255) at 23 hospitals in Spain, from February 2013 through February 2015...
August 2016: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/25914088/molecular-epidemiology-of-carbapenemase-producing-klebsiella-pneumoniae-in-a-hospital-in-madrid-successful-establishment-of-an-oxa-48-st11-clone
#13
Patricia Brañas, Jennifer Villa, Esther Viedma, Jesús Mingorance, M Angeles Orellana, Fernando Chaves
Here we report a retrospective clinical and molecular study conducted in a tertiary care facility in southern Madrid, Spain, from January 2009 to February 2014 to investigate the epidemiology of carbapenemase-producing Klebsiella pneumoniae (CPKp). Carbapenemase genes were identified in 97 non-duplicate K. pneumoniae isolates, including 59 harbouring blaOXA-48, 37 harbouring blaVIM-1 and 1 harbouring blaKPC-2. Pulsed-field gel electrophoresis (PFGE) analysis verified the presence of 20 different clonal types, whilst multilocus sequence typing (MLST) assigned the isolates to eight sequence types (STs)...
July 2015: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/25359914/draft-genome-sequence-of-colistin-only-susceptible-pseudomonas-aeruginosa-strain-st235-a-hypervirulent-high-risk-clone-in-spain
#14
Esther Viedma, Jenifer Villa, Carlos Juan, Antonio Oliver, Fernando Chaves
We report the genome of colistin-only-susceptible Pseudomonas aeruginosa strain ST235 (PA_ST235). This isolate was obtained in the setting of an outbreak in a tertiary hospital in Spain. This clone was apparently associated with a significantly higher mortality rate. The ST235 clone also appears to be associated with greater virulence.
2014: Genome Announcements
https://www.readbyqxmd.com/read/25081259/draft-whole-genome-sequence-of-oxa-48-producing-multidrug-resistant-klebsiella-pneumoniae-kp_st11_oxa-48
#15
Jennifer Villa, Esther Viedma, Patricia Brañas, Jesús Mingorance, Fernando Chaves
We present the draft genome sequence of a blood culture isolate of OXA-48-producing Klebsiella pneumoniae (sequence type 11 [ST11]) obtained in the course of a hospital outbreak in Spain. Sequence analysis showed 121 genes related to antibiotic and antiseptic resistance, including blaOXA-48, which was located in an IncL/M plasmid.
2014: Genome Announcements
https://www.readbyqxmd.com/read/24702943/multiclonal-spread-of-vim-1-producing-enterobacter-cloacae-isolates-associated-with-in624-and-in488-integrons-located-in-an-inchi2-plasmid
#16
Jennifer Villa, Esther Viedma, Patricia Brañas, M Angeles Orellana, Joaquín R Otero, Fernando Chaves
Over a 6-year period (2007-2012), the emergence of Enterobacter cloacae isolates resistant to β-lactams and with reduced susceptibility to carbapenems was observed in Hospital Universitario 12 de Octubre (Madrid, Spain). To determine the possible role of metallo-β-lactamases (MBLs) in the resistance profile of these isolates, a molecular and clinical epidemiological study was performed, including determination of patients' clinical characteristics, genetic diversity of strains, resistance mechanisms to carbapenems, and the genetic environment of VIM-1...
May 2014: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/24576447/tru-cut-needle-pleural-biopsy-and-cytology-as-the-initial-procedure-in-the-evaluation-of-pleural-effusion
#17
Maribel Botana Rial, Andrés Briones Gómez, José Ramón Ferrando Gabarda, José Fernando Cifuentes Ruiz, María Juliana Guarín Corredor, Nuria Manchego Frach, Enrique Cases Viedma
INTRODUCTION AND OBJECTIVES: The evaluation of pleural effusion (PE) includes various techniques, including pleural biopsy (PB). Our aim was to study the diagnostic yield of Tru-Cut needle PB (TCPB) and to define clinical/radiological situations in which TCPB might be indicated as an initial procedure. METHODOLOGY: Retrospective study of TCPB in a hospital centre (2010-2012). Cases of pleural lesions without effusion were excluded. Clinical and radiological variables, diagnostic yield, TCPB complications and factors associated with the diagnostic yield of the combination of TCPB and thoracocentesis as initial procedure were analysed...
August 2014: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/24492368/comparison-of-local-features-from-two-spanish-hospitals-reveals-common-and-specific-traits-at-multiple-levels-of-the-molecular-epidemiology-of-metallo-%C3%AE-lactamase-producing-pseudomonas-spp
#18
Esther Viedma, Vanesa Estepa, Carlos Juan, Jane Castillo-Vera, Beatriz Rojo-Bezares, Cristina Seral, Francisco Javier Castillo, Yolanda Sáenz, Carmen Torres, Fernando Chaves, Antonio Oliver
Twenty-seven well-characterized metallo-β-lactamase (MBL)-producing Pseudomonas strains from two distantly located hospitals were analyzed. The results revealed specific features defining the multilevel epidemiology of strains from each hospital in terms of species, clonality, predominance of high-risk clones, composition/diversity of integrons, and linkages of Tn402-related structures. Therefore, despite the global trends driving the epidemiology of MBL-producing Pseudomonas spp., the presence of local features has to be considered in order to understand this threat and implement proper control strategies...
2014: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/24296493/evaluation-of-genexpert-mtb-rif-for-the-detection-of-mycobacterium-tuberculosis-and-resistance-to-rifampin-in-clinical-specimens
#19
Eleonora Bunsow, María Jesús Ruiz-Serrano, Paula López Roa, Martha Kestler, Dario Garcia Viedma, Emilio Bouza
OBJECTIVES: GeneXpert (GX) is a novel real-time polymerase chain reaction assay for the simultaneous detection of Mycobacterium tuberculosis (MTB) and resistance to rifampin (RIF). We evaluated the performance of GX for direct detection of MTB in respiratory and non-respiratory specimens and assessed the ability of the assay to detect resistance to RIF in non-tuberculosis (TB) endemic country. METHODS: We analyzed 595 clinical samples in a 1550-bed tertiary hospital in Madrid, Spain...
April 2014: Journal of Infection
https://www.readbyqxmd.com/read/23975741/relationship-between-agr-dysfunction-and-reduced-vancomycin-susceptibility-in-methicillin-susceptible-staphylococcus-aureus-causing-bacteraemia
#20
Esther Viedma, Francisca Sanz, M Angeles Orellana, Rafael San Juan, Jose Maria Aguado, Joaquín R Otero, Fernando Chaves
OBJECTIVES: Limited data exist regarding the role of agr dysfunction in reducing susceptibility to vancomycin in methicillin-susceptible Staphylococcus aureus (MSSA). This study investigated the clinical and molecular epidemiology of MSSA causing bacteraemia, with emphasis on the reduced susceptibility to vancomycin (RSV) phenotype (MIC ≥ 1.5 mg/L) and its relationship with agr dysfunction. METHODS: All MSSA bloodstream isolates obtained at our hospital during 2010 were analysed...
January 2014: Journal of Antimicrobial Chemotherapy
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