keyword
MENU ▼
Read by QxMD icon Read
search

PCR malaria

keyword
https://www.readbyqxmd.com/read/28222769/plasticity-of-host-selection-by-malaria-vectors-of-papua-new-guinea
#1
John B Keven, Lisa Reimer, Michelle Katusele, Gussy Koimbu, Rebecca Vinit, Naomi Vincent, Edward Thomsen, David R Foran, Peter A Zimmerman, Edward D Walker
BACKGROUND: Host selection is an important determinant of vectorial capacity because malaria transmission increases when mosquitoes feed more on humans than non-humans. Host selection also affects the outcome of long-lasting insecticidal nets (LLIN). Despite the recent nationwide implementation of LLIN-based malaria control program in Papua New Guinea (PNG), little is known about the host selection of the local Anopheles vectors. This study investigated the host selection of Anopheles vectors in PNG...
February 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28219910/severe-plasmodium-knowlesi-with-dengue-coinfection
#2
Mohd Jazman Che Rahim, Nurashikin Mohammad, Alwi Muhd Besari, Wan Syamimee Wan Ghazali
We report a case of severe Plasmodium knowlesi and dengue coinfection in a previously healthy 59-year-old Malay man who presented with worsening shortness of breath, high-grade fever with chills and rigors, dry cough, myalgia, arthralgia, chest discomfort and poor appetite of 1 week duration. There was a history mosquito fogging around his neighbourhood in his hometown. Further history revealed that he went to a forest in Jeli (northern part of Kelantan) 3 weeks prior to the event. Initially he was treated as severe dengue with plasma leakage complicated with type 1 respiratory failure as evidenced by positive serum NS1-antigen and thrombocytopenia...
February 20, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28214533/the-effect-and-mechanism-of-inhibiting-glucose-6-phosphate-dehydrogenase-activity-on-the-proliferation-of-plasmodium-falciparum
#3
Zhiqiang Zhang, Xiaodan Chen, Chengrui Jiang, Zishui Fang, Yi Feng, Weiying Jiang
We screened >40,000 patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency and found that the G6PD Kaiping allele was under the most positive selection for fighting against malaria in the Chinese population. However, the mechanism is unknown. The current study was designed to investigate the anti-malarial effect and mechanism of G6PD deficiency. Dehydroepiandrosterone (DHEA) was utilised for inhibiting the G6PD activity of erythrocytes. Giemsa staining of blood smears and quantitative real-time PCR were used for the detection and quantification of Plasmodium falciparum infection...
February 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28196496/molecular-evidence-of-plasmodium-vivax-infection-in-duffy-negative-symptomatic-individuals-from-dschang-west-cameroon
#4
Gianluca Russo, Giovanni Faggioni, Giacomo Maria Paganotti, Ghyslaine Bruna Djeunang Dongho, Alice Pomponi, Riccardo De Santis, Gianpiero Tebano, Mpoame Mbida, Martin Sanou Sobze, Vincenzo Vullo, Giovanni Rezza, Florigio Romano Lista
BACKGROUND: Plasmodium vivax infection is known to be rare in West/Central Africa, the most accepted explanation being the lack of expression of erythroid Duffy antigen in the local human populations. Duffy negativity prevents the parasite to exploit the entry mechanism on the red blood cell surface. However, there are a growing number of reported vivax infections in Duffy-negative individuals. Data on P. vivax circulation in Cameroon are limited. The aim of the study was to evaluate the P...
February 14, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28187764/new-potential-plasmodium-brasilianum-hosts-tamarin-and-marmoset-monkeys-family-callitrichidae
#5
Denise A M Alvarenga, Anielle Pina-Costa, Cesare Bianco, Silvia B Moreira, Patricia Brasil, Alcides Pissinatti, Claudio T Daniel-Ribeiro, Cristiana F A Brito
BACKGROUND: Non-human primates (NHPs) as a source for Plasmodium infections in humans are a challenge for malaria elimination. In Brazil, two species of Plasmodium have been described infecting NHPs, Plasmodium brasilianum and Plasmodium simium. Both species are infective to man. Plasmodium brasilianum resembles morphologically, genetically and immunologically the human quartan Plasmodium malariae. Plasmodium brasilianum naturally infects species of non-human primates from all New World monkey families from a large geographic area...
February 10, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28183301/cellulose-filtration-of-blood-from-malaria-patients-for-improving-ex-vivo-growth-of-plasmodium-falciparum-parasites
#6
Sixbert I Mkumbaye, Daniel T R Minja, Jakob S Jespersen, Michael Alifrangis, Reginald A Kavishe, Steven B Mwakalinga, John P Lusingu, Thor G Theander, Thomas Lavstsen, Christian W Wang
BACKGROUND: Establishing in vitro Plasmodium falciparum culture lines from patient parasite isolates can offer deeper understanding of geographic variations of drug sensitivity and mechanisms of malaria pathogenesis and immunity. Cellulose column filtration of blood is an inexpensive, rapid and effective method for the removal of host factors, such as leucocytes and platelets, significantly improving the purification of parasite DNA in a blood sample. METHODS: In this study, the effect of cellulose column filtration of venous blood on the initial in vitro growth of P...
February 10, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28179695/a-pcr-method-based-on-18s-rrna-gene-for-detection-of-malaria-parasite-in-balochistan
#7
Zubeda Shahwani, Abdul Aleem, Nazeer Ahmed, Muhammad Mushtaq, Sarwat Afridi
OBJECTIVE: To establish a polymerase chain reaction method based on 18S ribosomal ribonucleic acid gene for the detection of plasmodium deoxyribonucleic acid in patients suffering from malaria symptoms. METHODS: This cross-sectional study was conducted from September 2013 to October 2014 in district Quetta of Pakistan's Balochistan province. Blood samples were collected from patients suffering from general symptoms of malaria. A polymerase chain reaction-based technique was applied for the diagnosis of malaria and detection of responsible species in the patients who were suspected to carry the parasite...
December 2016: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28173853/therapeutic-efficacy-and-effects-of-artemisinin-based-combination-treatments-on-uncomplicated-plasmodium-falciparum%C3%A2-malaria-associated-anaemia-in-nigerian-children-during-seven-years-of-adoption-as-first-line-treatments
#8
Akintunde Sowunmi, Kazeem Akano, Godwin Ntadom, Adejumoke I Ayede, Folasade O Ibironke, Temitope Aderoyeje, Elsie O Adewoye, Bayo Fatunmbi, Stephen Oguche, Henrietta U Okafor, Ismaila Watila, Martin Meremikwu, Philip Agomo, William Ogala, Chimere Agomo, Onikepe A Folarin, Grace O Gbotosho, Christian T Happi
BACKGROUND: Artemisinin-based combination treatments (ACTs) are the first-line treatments of uncomplicated Plasmodium falciparum malaria in many endemic areas but there are few evaluation of their efficacy in anaemic malarious children. METHODS: Therapeutic efficacy of 3-day regimens of artesunate-amodiaquine and artemether-lumefantrine was evaluated in 437 anaemic and 909 non-anaemic malarious children following treatment during a seven-year period (2008-2014)...
February 7, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28166235/development-and-clinical-performance-of-high-throughput-loop-mediated-isothermal-amplification-for-detection-of-malaria
#9
Rushini S Perera, Xavier C Ding, Frank Tully, James Oliver, Nigel Bright, David Bell, Peter L Chiodini, Iveth J Gonzalez, Spencer D Polley
BACKGROUND: Accurate and efficient detection of sub-microscopic malaria infections is crucial for enabling rapid treatment and interruption of transmission. Commercially available malaria LAMP kits have excellent diagnostic performance, though throughput is limited by the need to prepare samples individually. Here, we evaluate the clinical performance of a newly developed high throughput (HTP) sample processing system for use in conjunction with the Eiken malaria LAMP kit. METHODS: The HTP system utilised dried blood spots (DBS) and liquid whole blood (WB), with parallel sample processing of 94 samples per run...
2017: PloS One
https://www.readbyqxmd.com/read/28158202/high-plasmodium-falciparum-longitudinal-prevalence-is-associated-with-high-multiclonality-and-reduced-clinical-malaria-risk-in-a-seasonal-transmission-area-of-mali
#10
Yaw Adomako-Ankomah, Matthew S Chenoweth, Katelyn Durfee, Saibou Doumbia, Drissa Konate, Mory Doumbouya, Abdoul S Keita, Daria Nikolaeva, Gregory S Tullo, Jennifer M Anderson, Rick M Fairhurst, Rachel Daniels, Sarah K Volkman, Mahamadou Diakite, Kazutoyo Miura, Carole A Long
The effects of persistent Plasmodium falciparum (Pf) infection and multiclonality on subsequent risk of clinical malaria have been reported, but the relationship between these 2 parameters and their relative impacts on the clinical outcome of infection are not understood. A longitudinal cohort study was conducted in a seasonal and high-transmission area of Mali, in which 500 subjects aged 1-65 years were followed for 1 year. Blood samples were collected every 2 weeks, and incident malaria cases were diagnosed and treated...
2017: PloS One
https://www.readbyqxmd.com/read/28151566/efficacy-of-artesunate-sulfadoxine-pyrimethamine-and-artemether-lumefantrine-and-dhfr-and-dhps-mutations-in-somalia-evidence-for%C3%A2-updating-the-malaria-treatment-policy
#11
Marian Warsame, Abdikarim Hussein Hassan, Abdillahi Mohamed Hassan, Abdulkadir Mohamed Arale, Ali Mohamed Jibril, Said Abdulkadir Mohamud, Amy Barrette, Abdikarim Yusuf Muse, Fahmi Essa Yusuf Rania A Nada, Jamal Ghilan Hefzullah Amran
OBJECTIVE: To determine the therapeutic efficacy of artesunate + sulfadoxine/pyrimethamine (AS + SP) and artemether + lumefantrine (AL), and to investigate the presence of molecular mutations associated with resistance, to inform national malaria treatment policy. METHODS: One-arm prospective studies were conducted in three study sites in Somalia in 2013 and 2015 to evaluate the efficacy of AS + SP and AL among patients with uncomplicated falciparum malaria. Outcomes included clinical and parasitological response over 28 days, and the presence of dihydrofolate reductase (dfhr) and dihydropteroate synthase (dhps) and mutations...
February 2, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28148267/reduced-ex-vivo-susceptibility-of-plasmodium-falciparum-after-oral-artemether-lumefantrine-treatment-in-mali
#12
Souleymane Dama, Hamidou Niangaly, Amed Ouattara, Issaka Sagara, Sekou Sissoko, Oumar Bila Traore, Amadou Bamadio, Niawanlou Dara, Moussa Djimde, Mohamed Lamine Alhousseini, Siaka Goita, Hamma Maiga, Antoine Dara, Ogobara K Doumbo, Abdoulaye A Djimde
BACKGROUND: Artemisinin-based combination therapy is the recommended first-line treatment for uncomplicated falciparum malaria worldwide. However, recent studies conducted in Mali showed an increased frequency of recurrent parasitaemia following artemether-lumefantrine (AL) treatment. METHODS: Study samples were collected during a large WANECAM study. Ex-vivo Plasmodium falciparum sensitivity to artemether and lumefantrine was assessed using the tritiated hypoxanthine-based assay...
February 2, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28143518/submicroscopic-plasmodium-prevalence-in-relation-to-malaria-incidence-in-20-villages-in-western-cambodia
#13
Rupam Tripura, Thomas J Peto, Christianne C Veugen, Chea Nguon, Chan Davoeung, Nicola James, Mehul Dhorda, Richard J Maude, Jureeporn Duanguppama, Krittaya Patumrat, Mallika Imwong, Lorenz von Seidlein, Martin P Grobusch, Nicholas J White, Arjen M Dondorp
BACKGROUND: Cambodia has seen a marked reduction in the incidence of Plasmodium falciparum over the past decade without a corresponding decline in Plasmodium vivax incidence. It is unknown to what extent local transmission is sustained by a chain of clinical and sub-clinical infections or by continued re-introduction via migration. Using an ultrasensitive molecular technique, 20 villages in western Cambodia were surveyed to detect the low season prevalence of P. falciparum and P. vivax and local treatment records were reviewed...
January 31, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28129772/effect-of-a-short-course-of-iron-polymaltose-on-acquisition-of-malarial-parasitaemia-in-anaemic-indonesian-schoolchildren-a-randomized-trial
#14
Margaretta A Prasetyani, Quirijn de Mast, Robel Afeworki, Maria M M Kaisar, Difa Stefanie, Erliyani Sartono, Taniawati Supali, André J van der Ven
BACKGROUND: Concern exists about the safety of iron supplementation given to individuals in malarious areas. The possible unfavourable impact of iron supplementation on malaria might be less when slow-release iron compounds are used instead of ferrous salts, because no toxic non-transferrin bound iron is formed. The aim of this study was to determine the effect of iron supplementation using the slow-release iron compound iron polymaltose (IPM) on the acquisition of malarial parasitaemia...
January 28, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28127357/simplified-pan-species-real-time-pcr-based-detection-of-plasmodium-spp-in-blood-smear
#15
Gholamreza Hassanpour, Hossein Mirhendi, Mehdi Mohebali, Ahmad Raeisi, Hojjat Zeraati, Hossein Keshavarz
BACKGROUND: We aimed to quicken and simplify the detection of Plasmodium in blood samples by developing and testing a pan-Plasmodium real-time PCR for accurate screening of individuals suspected of malaria. METHODS: A single primer/probe set for pan-species Plasmodium-specific real time PCR targeting a conserved region of the small subunit 18S ribosomal DNA was designed and evaluated for rapid diagnosis and screening of malaria infections using dried blood smears...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28125696/molecular-detection-of-plasmodium-malariae-plasmodium-brasilianum-in-non-human-primates-in-captivity-in-costa-rica
#16
Alicia Fuentes-Ramírez, Mauricio Jiménez-Soto, Ruth Castro, Juan José Romero-Zuñiga, Gaby Dolz
One hundred and fifty-two blood samples of non-human primates of thirteen rescue centers in Costa Rica were analyzed to determine the presence of species of Plasmodium using thick blood smears, semi-nested multiplex polymerase chain reaction (SnM-PCR) for species differentiation, cloning and sequencing for confirmation. Using thick blood smears, two samples were determined to contain the Plasmodium malariae parasite, with SnM-PCR, a total of five (3.3%) samples were positive to P. malariae, cloning and sequencing confirmed both smear samples as P...
2017: PloS One
https://www.readbyqxmd.com/read/28122597/beyond-the-entomological-inoculation-rate-characterizing-multiple-blood-feeding-behavior-and-plasmodium-falciparum-multiplicity-of-infection-in-anopheles-mosquitoes-in-northern-zambia
#17
Smita Das, Mbanga Muleba, Jennifer C Stevenson, Julia C Pringle, Douglas E Norris
BACKGROUND: A commonly used measure of malaria transmission intensity is the entomological inoculation rate (EIR), defined as the product of the human biting rate (HBR) and sporozoite infection rate (SIR). The EIR excludes molecular parameters that may influence vector control and surveillance strategies. The purpose of this study was to investigate Anopheles multiple blood feeding behavior (MBF) and Plasmodium falciparum multiplicity of infection (MOI) within the mosquito host in Nchelenge District, northern Zambia...
January 26, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28119098/anti-inflammatory-effects-of-brucea-javanica-oil-emulsion-by-suppressing-nf-%C3%AE%C2%BAb-activation-on-dextran-sulfate-sodium-induced-ulcerative-colitis-in-mice
#18
Yan-Feng Huang, Jiang-Tao Zhou, Chang Qu, Yao-Xing Dou, Qiong-Hui Huang, Zhi-Xiu Lin, Yan-Fang Xian, Jian-Hui Xie, You-Liang Xie, Xiao-Ping Lai, Zi-Ren Su
ETHNOPHARMACOLOGICAL RELEVANCE: Brucea javanica is an important traditional medicinal herb used for the treatment of dysentery, malaria, inflammation and cancer in southeast Asia for many years. However, the anti-inflammatory mechanism of Brucea javanica in the treatment of dysentery (also known as ulcerative colitis, UC) has not been fully illuminated. Brucea javanica oil emulsion (BJOE) is the major active and most common application form of Brucea javanica oil (BJO), which has a variety of pharmacological activities...
January 21, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28119047/variable-number-of-tandem-repeats-of-9-plasmodium-vivax-genes-among-southeast-asian-isolates
#19
Bo Wang, Myat Htut Nyunt, Seung-Gyu Yun, Feng Lu, Yang Cheng, Jin-Hee Han, Kwon-Soo Ha, Won Sun Park, Seok-Ho Hong, Chae-Seung Lim, Jun Cao, Jetsumon Sattabongkot, Myat Phone Kyaw, Liwang Cui, Eun-Taek Han
The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P...
January 21, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28114990/recent-uptake-of-intermittent-preventive-treatment-during-pregnancy-with-sulfadoxine-pyrimethamine-is-associated-with-increased-prevalence-of-pfdhfr-mutations-in-bobo-dioulasso-burkina-faso
#20
Mamoudou Cisse, Gordon A Awandare, Alamissa Soulama, Halidou Tinto, Marie-Pierre Hayette, Robert T Guiguemdé
BACKGROUND: The impact of sulfadoxine-pyrimethamine (SP) used as intermittent preventive treatment during pregnancy (IPTp-SP) on mutant parasite selection has been poorly documented in Burkina Faso. This study sought first to explore the relationship between IPTp-SP and the presence of mutant parasites. Second, to assess the relationship between the mutant parasites and adverse pregnancy outcomes. METHODS: From September to December 2010, dried blood spots (DBS) were collected during antenatal care visits and at delivery from 109 pregnant women with microscopically confirmed falciparum malaria infection...
January 23, 2017: Malaria Journal
keyword
keyword
120160
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"