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https://www.readbyqxmd.com/read/28822205/comparison-of-effectiveness-of-two-different-artemisinin-based-combination-therapies-in-an-area-with-high-seasonal-transmission-of-malaria-in-burkina-faso
#1
Paul Sondo, Karim Derra, Seydou Diallo Nakanabo, Zekiba Tarnagda, Adama Kazienga, Innocent Valea, Herman Sorgho, Jean-Bosco Ouédraogo, Tinga Robert Guiguemdé, Halidou Tinto
In Sahelian countries such as Burkina Faso, malaria transmission is seasonal with a high incidence of transmission during the rainy season. This study aimed to compare the effectiveness of the two recommended treatments (Artemether-Lumefantrine and Artesunate-Amodiaquine) for uncomplicated malaria in Burkina Faso regarding this seasonal variation of malaria transmission. This is part of a randomized open label trial comparing the effectiveness and safety of Artemether-Lumefantrine versus Artesunate-Amodiaquine according to routine practice in Nanoro...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28820709/performance-of-a-high-sensitivity-rapid-diagnostic-test-for-plasmodium-falciparum-malaria-in-asymptomatic-individuals-from-uganda-and-myanmar-and-naive-human-challenge-infections
#2
Smita Das, Ihn Kyung Jang, Becky Barney, Roger Peck, John C Rek, Emmanuel Arinaitwe, Harriet Adrama, Maxwell Murphy, Mallika Imwong, Clare L Ling, Stephane Proux, Warat Haohankhunnatham, Melissa Rist, Annette M Seilie, Amelia Hanron, Glenda Daza, Ming Chang, Tomoka Nakamura, Michael Kalnoky, Paul Labarre, Sean C Murphy, James S McCarthy, Francois Nosten, Bryan Greenhouse, Sophie Allauzen, Gonzalo J Domingo
Sensitive field-deployable diagnostic tests can assist malaria programs in achieving elimination. The performance of a new Alere™ Malaria Ag P.f Ultra Sensitive rapid diagnostic test (uRDT) was compared with the currently available SD Bioline Malaria Ag P.f RDT in blood specimens from asymptomatic individuals in Nagongera, Uganda, and in a Karen Village, Myanmar, representative of high- and low-transmission areas, respectively, as well as in pretreatment specimens from study participants from four Plasmodium falciparum-induced blood-stage malaria (IBSM) studies...
August 7, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28818101/a-new-method-for-sequencing-the-hypervariable-plasmodium-falciparum-gene-var2csa-from-clinical-samples
#3
Antoine Dara, Mark A Travassos, Matthew Adams, Sarah Schaffer DeRoo, Elliott F Drábek, Sonia Agrawal, Miriam K Laufer, Christopher V Plowe, Joana C Silva
BACKGROUND: VAR2CSA, a member of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family, mediates the binding of P. falciparum-infected erythrocytes to chondroitin sulfate A, a surface-associated molecule expressed in placental cells, and plays a central role in the pathogenesis of placental malaria. VAR2CSA is a target of naturally acquired immunity and, as such, is a leading vaccine candidate against placental malaria. This protein is very polymorphic and technically challenging to sequence...
August 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28810865/cryptic-plasmodium-ovale-concurrent-with-mixed-plasmodium-falciparum-and-plasmodium-malariae-infection-in-two-children-from-central-african-republic
#4
Cynthia Bichara, Philippe Flahaut, Damien Costa, Anne-Lise Bienvenu, Stephane Picot, Gilles Gargala
BACKGROUND: Since several malaria parasite species are usually present in a particular area, co-infections with more than one species of Plasmodium are more likely to occur in humans infected in these areas. In many mixed infections, parasite densities of the cryptic species may be low and often not recognized in clinical practice. CASE PRESENTATION: Two children (3 and 6 years old) adopted recently from Central African Republic were admitted to hospital because of intermittent fever...
August 15, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28800735/life-span-of-in-vitro-differentiated-plasmodium-falciparum-gametocytes
#5
Tamirat Gebru, Albert Lalremruata, Peter G Kremsner, Benjamin Mordmüller, Jana Held
BACKGROUND: The sexual stages (gametocytes) of Plasmodium falciparum do not directly contribute to the pathology of malaria but are essential for transmission of the parasite from the human host to the mosquito. Mature gametocytes circulate in infected human blood for several days and their circulation time has been modelled mathematically from data of previous in vivo studies. This is the first time that longevity of gametocytes is studied experimentally in vitro. METHODS: The in vitro longevity of P...
August 11, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28800640/knockdown-of-the-plasmodium-falciparum-surfin4-1-antigen-leads-to-an-increase-of-its-cognate-transcript
#6
Tatiane Macedo-Silva, Rosana Beatriz Duque Araujo, Kamila Anna Meissner, Wesley Luzetti Fotoran, Márcia Melo Medeiros, Mauro Ferreira de Azevedo, Gerhard Wunderlich
The genome of the malaria parasite Plasmodium falciparum contains the surf gene family which encodes large transmembrane proteins of unknown function. While some surf alleles appear to be expressed in sexual stages, others occur in asexual blood stage forms and may be associated to virulence-associated processes and undergo transcriptional switching. We accessed the transcription of surf genes along multiple invasions by real time PCR. Based on the observation of persistent expression of gene surf4.1, we created a parasite line which expresses a conditionally destabilized SURFIN4...
2017: PloS One
https://www.readbyqxmd.com/read/28797269/a-reduction-in-malaria-transmission-intensity-in-northern-ghana-after-7%C3%A2-years-of-indoor-residual-spraying
#7
Sylvester Coleman, Samuel K Dadzie, Aklilu Seyoum, Yemane Yihdego, Peter Mumba, Dereje Dengela, Philip Ricks, Kristen George, Christen Fornadel, Daniel Szumlas, Paul Psychas, Jacob Williams, Maxwell A Appawu, Daniel A Boakye
BACKGROUND: Indoor residual spraying (IRS) is being implemented as one of the malaria prevention methods in the Northern Region of Ghana. Changes in longevity, sporozoite and entomological inoculation rates (EIRs) of major malaria vectors were monitored to assess the impact of IRS in selected districts. METHODS: Monthly human landing catches (HLCs) were used to collect mosquitoes from sentinel sites in three adjacent districts between July 2009 and December 2014: Savelugu Nanton (SND) where IRS had been implemented from 2008 to 2014; Tolon Kumbungu (TKD) where IRS had been implemented between 2008 and 2012 and Tamale Metropolis (TML) with no history of IRS...
August 10, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28797261/evaluation-of-carestart%C3%A2-malaria-hrp2-pldh-pf-pan-combo-test-in-a-malaria-low-transmission-region-of-senegal
#8
Mamadou Alpha Diallo, Khadim Diongue, Mouhamadou Ndiaye, Amy Gaye, Awa Deme, Aida S Badiane, Daouda Ndiaye
BACKGROUND: This study was initiated from the observation that prevalence of malaria obtained with rapid diagnostic test (RDT) (CareStart™Malaria HRP2/pLDH Combo Test) was higher than in microscopy in a malaria low transmission area of Senegal. PCR was then performed to evaluate the performance of the RDT compared to microscopy in clinical settings. METHODS: The study included 215 patients suspected of malaria in two peri-urban area of Dakar. Finger-pick blood samples were tested using RDT (CareStart™Malaria HRP2/pLDH Combo Test)...
August 10, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28797235/polymorphisms-of-plasmodium-falciparum-k13-propeller-gene-among-migrant-workers-returning-to-henan-province-china-from-africa
#9
Chengyun Yang, Hongwei Zhang, Ruimin Zhou, Dan Qian, Ying Liu, Yuling Zhao, Suhua Li, Bianli Xu
BACKGROUND: Henan Province has been in the malaria elimination stage, with all reports of the disease being imported since 2012 and over 90% coming from Africa. Surveillance and population studies are essential for the early detection and subsequent prevention of the spread of drug resistance. The K13-propeller gene was recently identified as a proposed molecular marker of artemisinin (ART) resistance. In this study, we detected mutations of the K13-propeller gene in samples taken from imported malaria cases in Henan Province from 2012 to 2015...
August 10, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28796820/identification-of-plasmodium-spp-in-neotropical-primates-of-maranhense-amazon-in-northeast-brazil
#10
Mayra Araguaia Pereira Figueiredo, Silvia Maria Di Santi, Wilson Gómez Manrique, Marcos Rogério André, Rosangela Zacarias Machado
In the Brazilian Amazon region, malaria caused by Plasmodium malariae is considered to be a zoonosis because of cross-transfer of the parasite between humans and Neotropical primates. To contribute information on this issue, we investigated occurrences of natural infection with Plasmodium sp. among Neotropical primates in the Maranhense Amazon (Amazon region of the state of Maranhão), in the northeastern region of Brazil. Blood samples were collected from 161 Neotropical primates of six species that were caught in an environmental reserve (Sítio Aguahy) and from captive primates (CETAS-Wildlife Screening Center, municipality of São Luís), in Maranhão...
2017: PloS One
https://www.readbyqxmd.com/read/28795223/high-resolution-melting-pcr-assay-applicable-for-diagnostics-and-screening-studies-allowing-detection-and-differentiation-of-several-babesia-spp-infecting-humans-and-animals
#11
Wioletta Rozej-Bielicka, Aleksander Masny, Elzbieta Golab
The goal of the study was to design a single tube PCR test for detection and differentiation of Babesia species in DNA samples obtained from diverse biological materials. A multiplex, single tube PCR test was designed for amplification of approximately 400 bp region of the Babesia 18S rRNA gene. Universal primers were designed to match DNA of multiple Babesia spp. and to have low levels of similarity to DNA sequences of other intracellular protozoa and Babesia hosts. The PCR products amplified from Babesia DNA isolated from human, dog, rodent, deer, and tick samples were subjected to high-resolution melting analysis for Babesia species identification...
August 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28793894/prevalence-of-polymorphisms-in-glucose-6-phosphate-dehydrogenase-sickle-haemoglobin-and-nitric-oxide-synthase-genes-and-their-relationship-with-incidence-of-uncomplicated-malaria-in-iganga-uganda
#12
Catherine Nassozi Lwanira, Fred Kironde, Mark Kaddumukasa, Göte Swedberg
BACKGROUND: Host genetics play an important role in Plasmodium falciparum malaria susceptibility. However, information on host genetic factors and their relationships with malaria in the vaccine trial site of Iganga, Uganda is limited. The main objective of this study was to determine the prevalence of selected host genetic markers and their relationship to malaria incidence in the vaccine trial site of Iganga, Uganda. In a 1-year longitudinal cohort study, 423 children aged below 9 years were recruited and their malaria episodes were investigated...
August 9, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28792284/neurological-involvement-associated-with-plasmodium-vivax-malaria-from-pakistan
#13
Yousaf Abdullah Khan, Usman Hameed Mian, Najia Karim Ghanchi, Ali Bin Sarwar Zubairi, Mohammad Asim Beg
Plasmodium vivax is the most common specie causing malaria outside Africa with approximately 13.8 million reported cases worldwide. We report case of P. vivax infection with cerebral involvement. A nine year old boy presented with high grade fever accompanied by projectile vomiting and abnormal behavior later he developed seizures, shock, and unconsciousness. P. vivax monoinfection was diagnosed based on peripheral smears and PCR. After antimalarial therapy, patient made full recovery. Current case highlights increasing trend of cerebral complications caused by P...
January 1, 2017: Tropical Doctor
https://www.readbyqxmd.com/read/28789682/additional-burden-of-asymptomatic-and-sub-patent-malaria-infections-during-low-transmission-season-in-forested-tribal-villages-in-chhattisgarh-india
#14
Mehul Kumar Chourasia, Kamaraju Raghavendra, Rajendra M Bhatt, Dipak Kumar Swain, Hemraj M Meshram, Jayant K Meshram, Shrity Suman, Vinita Dubey, Gyanendra Singh, Kona Madhavinadha Prasad, Immo Kleinschmidt
BACKGROUND: The burden of sub-patent malaria is difficult to recognize in low endemic areas due to limitation of diagnostic tools, and techniques. Polymerase chain reaction (PCR), a molecular based technique, is one of the key methods for detection of low parasite density infections. The study objective was to assess the additional burden of asymptomatic and sub-patent malaria infection among tribal populations inhabiting three endemic villages in Keshkal sub-district, Chhattisgarh, India...
August 8, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28777043/diagnostic-potential-of-monoclonal-antibodies-developed-against-c-terminal-polypeptide-of-p-falciparum-histidine-rich-protein2-pfhrp2-in-malaria-infected-patients-from-india
#15
Reena Verma, Sara Chandy, N S Jayaprakash, Anand Manoharan, M A Vijayalakshmi, Krishnan Venkataraman
Malaria, caused by Plasmodium falciparum has become a major health burden in most tropical and developing countries. P. falciparum Histidine Rich Protein2 (PfHRP2), which exhibits polymorphism, is being widely used as a diagnostic marker. Recently, we reported the development of monoclonal antibodies against conserved C-terminal 105 amino acids of PfHRP2 for malaria diagnosis. Now, in this study, the diagnostic performance of two anti-C-terminal PfHRP2 mAbs (b10c1 and Aa3c10) were evaluated with 100 blood samples from clinically identified malaria patients from seven different geographical centers in India...
August 4, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28774303/comparison-of-anti-malarial-drugs-efficacy-in-the-treatment-of-uncomplicated-malaria-in-african-children-and-adults-using-network-meta-analysis
#16
Solange Whegang Youdom, Rachida Tahar, Leonardo K Basco
BACKGROUND: Artemisinin-based combination therapy (ACT) and novel drug combinations are available and used in African countries to treat uncomplicated malaria. Network meta-analysis methods are rarely and poorly applied for the comparison of their efficacies. This method was applied on a set of randomized controlled trials to illustrate its usefulness. METHODS: A literature review available in Pubmed was conducted in July 2016. Eligible studies, conducted in sub-Saharan Africa, published between 2002 and 2016, focused on randomized controlled trials of at least two artemisinin-based combinations to treat uncomplicated malaria in children and adults...
August 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28771615/substantial-asymptomatic-submicroscopic-plasmodium-carriage-during-dry-season-in-low-transmission-areas-in-senegal-implications-for-malaria-control-and-elimination
#17
Makhtar Niang, Laty Gaye Thiam, Rokhaya Sane, Nafissatou Diagne, Cheikh Talla, Souleymane Doucoure, Joseph Faye, Fode Diop, Abdoulaye Badiane, Babacar Diouf, Diogop Camara, Fatoumata Diene-Sarr, Cheikh Sokhna, Vincent Richard, Aissatou Toure-Balde
BACKGROUND: In the progress towards malaria elimination, the accurate diagnosis of low-density asymptomatic infections is critical. Low-density asymptomatic submicroscopic malaria infections may act as silent reservoirs that maintain low-level residual malaria transmission in the community. Light microscopy, the gold standard in malaria diagnosis lacks the sensitivity to detect low-level parasitaemia. In this study, the presence and prevalence of submicroscopic Plasmodium carriage were investigated to estimate the parasites reservoir among asymptomatic individuals living in low transmission areas in Dielmo and Ndiop, Senegal during the dry season...
2017: PloS One
https://www.readbyqxmd.com/read/28768730/detection-of-plasmodium-spp-infection-by-the-illumigene%C3%A2-malaria-assay-compared-to-reference-microscopy-and-real-time-pcr
#18
Candace Rypien, Barbara Chow, Wilson Chan, Deirdre Church, Dylan R Pillai
Malaria is one of the leading causes of infectious disease in returning travelers from the tropics. Diagnosis of malaria is typically performed by examination of Giemsa-stained thick and thin peripheral blood smears, which is time-consuming, labor-intensive and requires high levels of proficiency. Alternatively, loop-mediated isothermal amplification (LAMP) is a new molecular method, which is rapid, sensitive, and requires less capital equipment and technological training. We conducted a retrospective study comparing two formats of a commercial LAMP assay (Meridian illumigene® Malaria [M] and Malaria Plus [MP]) versus reference microscopy on archived blood specimens (n=140) obtained from unique returning travelers suspected of having malaria...
August 2, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28764710/host-attraction-and-biting-behaviour-of-anopheles-mosquitoes-in-south-halmahera-indonesia
#19
Brandyce St Laurent, Timothy A Burton, Siti Zubaidah, Helen C Miller, Puji B Asih, Amirullah Baharuddin, Sully Kosasih, Shinta, Saya Firman, William A Hawley, Thomas R Burkot, Din Syafruddin, Supratman Sukowati, Frank H Collins, Neil F Lobo
BACKGROUND: Indonesia is home to a variety of malaria vectors whose specific bionomic traits remain largely uncharacterized. Species-specific behaviours, such as host feeding preferences, impact the dynamics of malaria transmission and the effectiveness of vector control interventions. METHODS: To examine species-specific host attraction and feeding behaviours, a Latin square design was used to compare Anopheles mosquitoes attracted to human, cow, and goat-baited tents...
August 2, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28761783/quantitative-real-time-pcr-analysis-of-anopheles-dirus-tep1-and-nos-during-plasmodium-berghei-infection-using-three-reference-genes
#20
Jonathan W K Liew, Mun Yik Fong, Yee Ling Lau
Quantitative reverse transcription PCR (qRT-PCR) has been an integral part of characterizing the immunity of Anopheles mosquitoes towards Plasmodium invasion. Two anti-Plasmodium factors of Anopheles, thioester-containing protein 1 (TEP1) and nitric oxide synthase (NOS), play a role in the refractoriness of Anopheles towards Plasmodium infection and are generally expressed during infection. However, these are less studied in Anopheles dirus, a dominant malaria vector in Southeast Asia. Furthermore, most studies used a single reference gene for normalization during gene expression analysis without proper validation...
2017: PeerJ
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