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PCR malaria

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https://www.readbyqxmd.com/read/28522856/diagnosing-congenital-malaria-in-a-high-transmission-setting-clinical-relevance-and-usefulness-of-p-falciparum-hrp2-based-testing
#1
Hamtandi Magloire Natama, Delwendé Florence Ouedraogo, Hermann Sorgho, Eduard Rovira-Vallbona, Elisa Serra-Casas, M Athanase Somé, Maminata Coulibaly-Traoré, Petra F Mens, Luc Kestens, Halidou Tinto, Anna Rosanas-Urgell
Congenital malaria diagnosis is challenging due to frequently observed low parasite density infections, while their clinical relevance during early infancy is not well characterized. In Nanoro health district (Burkina Faso), we determined the prevalence of congenital malaria by real-time quantitative PCR and we assessed the performance of rapid diagnosis test (RDT) and light microscopy (LM) to detect Plasmodium falciparum infections in cord-blood samples. In addition, we examined the usefulness of P. falciparum Histidine Rich Protein2 (PfHRP2) as surrogate biomarker of infection and explored association between congenital malaria and clinical outcomes...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28511713/artemisinin-based-combination-therapy-in-pregnant-women-in-zambia-efficacy-safety-and-risk-of-recurrent-malaria
#2
Michael Nambozi, Jean-Bertin Bukasa Kabuya, Sebastian Hachizovu, David Mwakazanga, Joyce Mulenga, Webster Kasongo, Jozefien Buyze, Modest Mulenga, Jean-Pierre Van Geertruyden, Umberto D'Alessandro
BACKGROUND: In Zambia, malaria is one of the leading causes of morbidity and mortality, especially among under five children and pregnant women. For the latter, the World Health Organization recommends the use of artemisinin-based combination therapy (ACT) in the second and third trimester of pregnancy. In a context of limited information on ACT, the safety and efficacy of three combinations, namely artemether-lumefantrine (AL), mefloquine-artesunate (MQAS) and dihydroartemisinin-piperaquine (DHAPQ) were assessed in pregnant women with malaria...
May 16, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28510593/no-serological-evidence-for-zika-virus-infection-and-low-specificity-for-anti-zika-virus-elisa-in-malaria-positive-individuals-among-pregnant-women-from-madagascar-in-2010
#3
Norbert Georg Schwarz, Eva Mertens, Doris Winter, Oumou Maiga-Ascofaré, Denise Dekker, Stephanie Jansen, Dennis Tappe, Njary Randriamampionona, Jürgen May, Raphael Rakotozandrindrainy, Jonas Schmidt-Chanasit
It was previously reported that a malaria infection may interfere with the specificity of a commercial ELISA test against Zika virus (ZIKV). We analyzed 1,216 plasma samples from healthy, pregnant women collected in two sites in Madagascar in 2010 for ZIKV antibodies using a commercial ELISA and for Plasmodium infection by PCR. This screen revealed six putative ZIKV-positive samples by ELISA. These results could not be confirmed by indirect immunofluorescence assays or virus neutralization tests. Four of these six samples were also positive for P...
2017: PloS One
https://www.readbyqxmd.com/read/28507350/amplification-of-mitochondrial-dna-for-detection-of-plasmodiumvivax-in-balochistan
#4
Muhammad Naeem Shahwani, Samia Nisar, Abdul Aleem, Marina Panezai, Sarwat Afridi, Shaukat Iqbal Malik
OBJECTIVE: To access a new step using PCR to amplify the targeted mtDNA sequence for detecting specifically Plasmodium vivax and its co-infections, false positive and false negative results with Plasmodium falciparum. METHODS: In this study we have standardized a new technical approach in which the target mitochondrial DNA sequence (mtDNA) was amplified by using a PCR technique as a tool to detect Plasmodium spp. Species specific primers were designed to hybridize with cytochrome c oxidase gene of P...
May 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28506038/pcr-rflp-for-rapid-subtyping-of-plasmodium-vivax-korean-isolates
#5
Jung-Mi Kang, Jinyoung Lee, Tae Im Kim, Eun-Ha Koh, Tong-Soo Kim, Woon-Mok Sohn, Byoung-Kuk Na
Vivax malaria reemerged in Korea in 1993 and the outbreak has been continued with fluctuating numbers of annual indigenous cases. Understanding the nature of the genetic population of Plasmodium vivax circulating in Korea is beneficial for the knowledge of the nationwide parasite heterogeneity and in the implementation of malaria control programs in the country. Previously, we analyzed polymorphic nature of merozoite surface protein-1 (MSP-1) and MSP-3α in Korean P. vivax population and identified the Korean P...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28504441/vector-bionomics-and-malaria-transmission-along-the-thailand-myanmar-border-a-baseline-entomological-survey
#6
N Kwansomboon, V Chaumeau, P Kittiphanakun, D Cerqueira, V Corbel, T Chareonviriyaphap
Baseline entomological surveys were conducted in four sentinel sites along the Thailand-Myanmar border to address vector bionomics and malaria transmission in the context of a study on malaria elimination. Adult Anopheles mosquitoes were collected using human-landing catch and cow-bait collection in four villages during the rainy season from May-June, 2013. Mosquitoes were identified to species level by morphological characters and by AS-PCR. Sporozoite indexes were determined on head/thoraces of primary and secondary malaria vectors using real-time PCR...
June 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/28497225/development-of-a-rapid-hrm-qpcr-for-the-diagnosis-of-the-four-most-prevalent-plasmodium-lineages-in-new-zealand
#7
E R Schoener, S Hunter, L Howe
Although wildlife rehabilitation and translocations are important tools in wildlife conservation in New Zealand, disease screening of birds has not been standardized. Additionally, the results of the screening programmes are often difficult to interpret due to missing disease data in resident or translocating avian populations. Molecular methods have become the most widespread method for diagnosing avian malaria (Plasmodium spp.) infections. However, these methods can be time-consuming, expensive and are less specific in diagnosing mixed infections...
May 11, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28490339/distribution-and-phylogeny-of-wolbachia-strains-in-wild-mosquito-populations-in-sri-lanka
#8
N W Nalaka P Nugapola, W A Priyanka P De Silva, S H P Parakrama Karunaratne
BACKGROUND: Wolbachia are a group of maternally inherited intracellular bacteria known to be widespread among arthropods. Infections with Wolbachia cause declines of host populations, and also induce host resistance to a wide range of pathogens. Over the past few decades, researchers were curious to use Wolbachia as a biological tool to control mosquito vectors. During the present study, assessment of the prevalence of Wolbachia infections among wild mosquito populations in Sri Lanka where mosquito-borne diseases are a major health concern, was carried out for the first time...
May 10, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28489935/indoor-residual-spraying-with-micro-encapsulated-pirimiphos-methyl-actellic%C3%A2-300cs-against-malaria-vectors-in-the-lake-victoria-basin-tanzania
#9
Fabian M Mashauri, Alphaxard Manjurano, Safari Kinung'hi, Jackline Martine, Eric Lyimo, Coleman Kishamawe, Chacha Ndege, Mahdi M Ramsan, Adeline Chan, Charles D Mwalimu, John Changalucha, Stephen Magesa
BACKGROUND: The indoor residual spraying programme for malaria vectors control was implemented in four districts of the Lake Victoria basin of Tanzania namely Ukerewe, Sengerema, Rorya andSerengeti. Entomological monitoring activities were implemented in one sentinel village in each district to evaluate the efficacy of pirimiphos-methyl 300 CS sprayed on different wall surfaces and its impact against malaria vectors post-IRS intervention. METHODS: The residual decay rate of p-methyl 300 CS applied at a target dosage of 1g a...
2017: PloS One
https://www.readbyqxmd.com/read/28482182/role-of-mal-gene-polymorphism-rs8177374-in-modulation-of-malaria-severity-in-the-pakistani-population
#10
Asima Rani, Syed Kashif Nawaz, Shazia Irfan, Muhammad Arshad, Razia Bashir, Najma Shaheen
INTRODUCTION: The present study was designed to investigate the association between rs8177374 polymorphism and malaria symptoms due to exposure of Plasmodium vivax and Plasmodium falciparum. MATERIALS AND METHODS: A total of 454 samples were included in the study (228 malaria patients and 226 healthy individuals). Malaria patients, divided into P. vivax and P. falciparum groups on the basis of the causative species of Plasmodium, were categorized into mild and severe on the basis of clinical outcomes according to WHO criteria...
May 5, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28479922/laboratory-diagnosis-of-malaria-comparison-of-manual-and-automated-diagnostic-tests
#11
Samina Naz Mukry, Madiha Saud, Gul Sufaida, Kashif Shaikh, Arshi Naz, Tahir Sultan Shamsi
Malaria is the second most prevalent disease in Pakistan resulting in ~30,000 annual deaths. In endemic countries like Pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes. Malarial parasite was screened in 128 malaria suspected patients and 150 healthy controls, by species-specific PCR, microscopy of blood smears, hemoanalyzer Sysmex XE-2100, and rapid test devices (First Response Malaria® and ICT Malaria Combo®). The microscopy detected MP in 126 samples (parasite load/µl 386-53712/µl); 71...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28477100/multidisciplinary-re-description-of-plasmodium-novyella-paranucleophilum-in-brazilian-wild-birds-of-the-atlantic-forest-kept-in-captivity
#12
Raquel Tostes, Roberto Júnio Pedroso Dias, Isabel Martinele, Marcus Vinicius Xavier Senra, Marta D'Agosto, Carlos Luiz Massard
Haemosporidian blood parasites of the Plasmodium genus are the causative agents of avian malaria in many parts of the world. Despite the great diversity of Brazilian avifauna, few studies have been conducted to examine the haemosporidians of wild birds found in the Brazilian Atlantic Forest, especially those kept in captivity. This study aimed to re-examine and further characterize the South American avian parasite Plasmodium paranucleophilum using a multidisciplinary approach. Blood samples were collected from 68 captive birds representing 15 species found in the Atlantic Forest of southeastern Brazil...
May 5, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28465622/sequence-variation-in-plasmodium-falciparum-histidine-rich-proteins-2-and-3-in-indian-isolates-implications-for-malaria-rapid-diagnostic-test-performance
#13
Praveen Kumar Bharti, Himanshu Singh Chandel, Sri Krishna, Shrikant Nema, Amreen Ahmad, Venkatachalam Udhayakumar, Neeru Singh
Commercial malaria rapid diagnostic tests (RDTs) detect P. falciparum histidine rich protein 2 (PfHRP2) and cross react with PfHRP3, a structural homologue. Here, we analysed natural variations in PfHRP2 and PfHRP3 sequences from Indian isolates and correlated these variations with RDT reactivity. A total 1392 P. falciparum positive samples collected from eight endemic states were PCR amplified for Pfhrp2 and Pfhrp3 genes and were sequenced. The deduced protein sequences were analysed for repeat variations and correlated with RDT reactivity...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28465550/in-vivo-efficacy-of-chloroquine-to-clear-asymptomatic-infections-in-mozambican-adults-a-randomized-placebo-controlled-trial-with-implications-for-elimination-strategies
#14
Beatriz Galatas, Lidia Nhamussua, Baltazar Candrinho, Lurdes Mabote, Pau Cisteró, Himanshu Gupta, Regina Rabinovich, Clara Menéndez, Eusebio Macete, Francisco Saute, Alfredo Mayor, Pedro Alonso, Quique Bassat, Pedro Aide
Recent reports regarding the re-emergence of parasite sensitivity to chloroquine call for a new consideration of this drug as an interesting complementary tool in malaria elimination efforts, given its good safety profile and long half-life. A randomized (2:1), single-blind, placebo-controlled trial was conducted in Manhiça, Mozambique, to assess the in-vivo efficacy of chloroquine to clear plasmodium falciparum (Pf) asymptomatic infections. Primary study endpoint was the rate of adequate and parasitological response (ACPR) to therapy on day 28 (PCR-corrected)...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28460834/asymptomatic-plasmodial-infection-in-colombian-pregnant-women
#15
Jaime Carmona-Fonseca, Olga M Agudelo, Eliana M Arango
Information about asymptomatic plasmodial infection is scarce in the world, and the current antimalarial program goals (control, elimination, and eradication) demand this evidence to be well documented in different populations and malaria transmission settings. This study aimed to measure the prevalence of API in Colombian pregnant women at delivery. A retrospective prevalence survey was used. Women were recruited at hospital obstetric facility in each of the municipalities of Turbo, Necoclí in Antioquia department, and Puerto Libertador in Córdoba department...
April 28, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28454537/increase-in-the-prevalence-of-mutations-associated-with-sulfadoxine-pyrimethamine-resistance-in-plasmodium-falciparum-isolates-collected-from-early-to-late-pregnancy-in-nanoro-burkina-faso
#16
Esmée Ruizendaal, Marc C Tahita, Ronald B Geskus, Inge Versteeg, Susana Scott, Umberto d'Alessandro, Palpouguini Lompo, Karim Derra, Maminata Traore-Coulibaly, Menno D de Jong, Henk D F H Schallig, Halidou Tinto, Petra F Mens
BACKGROUND: Pregnant women are a high-risk group for Plasmodium falciparum infections, which may result in maternal anaemia and low birth weight newborns, among other adverse birth outcomes. Intermittent preventive treatment with sulfadoxine-pyrimethamine during pregnancy (IPTp-SP) is widely implemented to prevent these negative effects of malaria. However, resistance against SP by P. falciparum may decrease efficacy of IPTp-SP. Combinations of point mutations in the dhps (codons A437, K540) and dhfr genes (codons N51, C59, S108) of P...
April 28, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28442114/nested-multiplex-pcr-for-identification-and-detection-of-human-plasmodium-species-including-plasmodium-knowlesi
#17
Maria Miguel-Oteo, Adela I Jiram, Thuy H Ta-Tang, Marta Lanza, Shamilah Hisam, José M Rubio
OBJECTIVE: To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of Plasmodium causing human malaria. METHODS: In this study the nested multiplex malaria PCR was redesigned, targeting the 18S rRNA gene, to identify the fifth human Plasmodium species, Plasmodium knowlesi, together with the other human Plasmodium (Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale and Plasmodium malariae) by amplified fragment size using only two amplification processes and including an internal reaction control to avoid false negatives...
March 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28431115/spatial-distribution-of-falciparum-malaria-infections-in-zanzibar-implications-for-focal-drug-administration-strategies-targeting-asymptomatic-parasite-carriers
#18
Anders Björkman, Jackie Cook, Hugh Sturrock, Mwinyi Msellem, Abdullah Ali, Weiping Xu, Fabrizio Molteni, Roly Gosling, Chris Drakeley, Andreas Mårtensson
Background.: Optimal use of mass/targeted screen-and-treat or mass or focal drug administration as malaria elimination strategies remains unclear. We therefore studied spatial distribution of Plasmodium falciparum infections to compare simulated effects of these strategies on reducing the parasite reservoir in a pre-elimination setting. Methods.: P. falciparum rapid diagnostic tests (RDTs) and molecular (polymerase chain reaction [PCR]) and serological (enzyme-linked immunosorbent assay) analyses were performed on finger-prick blood samples from a population-based survey in 3 adjacent communities...
May 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28427455/comparison-of-methods-for-detecting-asymptomatic-malaria-infections-in-the-china-myanmar-border-area
#19
Yonghong Zhao, Yan Zhao, Yanmin Lv, Fei Liu, Qinghui Wang, Peipei Li, Zhenjun Zhao, Yingjie Liu, Liwang Cui, Qi Fan, Yaming Cao
BACKGROUND: Sensitive methods for detecting asymptomatic malaria infections are essential for identifying potential transmission reservoirs and obtaining an accurate assessment of malaria epidemiology in low-endemicity areas aiming to eliminate malaria. PCR techniques to detect parasite nucleic acids (DNA or RNA) are among the most commonly used molecular methods. However, most of these methods are of low throughput and cannot be used for large-scale molecular epidemiological studies...
April 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28427409/efficacies-of-dha-ppq-and-as-sp-in-patients-with-uncomplicated-plasmodium-falciparum-malaria-in-an-area-of-an-unstable-seasonal-transmission-in-sudan
#20
Abdelrahim O Mohamed, Muzamil M Abdel Hamid, Omer S Mohamed, Nuha S Elkando, Abdelmaroof Suliman, Mariam A Adam, Fahad Awad Ali Elnour, Elfatih M Malik
BACKGROUND: Artemisinin-based combination therapy (ACT), together with other control measures, have reduced the burden of falciparum malaria in sub-Saharan countries, including Sudan. Sudan adopted ACT in 2004 with a remarkable reduction in mortality due to falciparum malaria. However, emergence of resistance to the first-line treatment artesunate and sulfadoxine/pyrimethamine (AS/SP) has created new challenges to the control of malaria in Sudan. A search for an alternative drug of choice for treating uncomplicated malaria has become inevitable...
April 20, 2017: Malaria Journal
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