keyword
MENU ▼
Read by QxMD icon Read
search

PCR malaria

keyword
https://www.readbyqxmd.com/read/29216395/a-randomized-trial-of-the-prophylactic-activity-of-dsm265-against-pre-erythrocytic-plasmodium-falciparum-controlled-human-malaria-infection-by-mosquito-bites-and-direct-venous-inoculation
#1
Sean C Murphy, Elizabeth R Duke, Kelly J Shipman, Ryan L Jensen, Youyi Fong, Sue Ferguson, Holly E Janes, Kevin Gillespie, Annette M Seilie, Amelia E Hanron, Laurie Rinn, Matthew Fishbaugher, Tracie VonGoedert, Emma Fritzen, Stefan H Kappe, Ming Chang, Jason C Sousa, Sean R Marcsisin, Stephan Chalon, Stephan Duparc, Nicola Kerr, Jörg J Möhrle, Nicole Andenmatten, Thomas Rueckle, James G Kublin
Background: DSM265 is a selective inhibitor of Plasmodium dihydroorotate dehydrogenase that fully protected against controlled human malarial infection (CHMI) by direct venous inoculation of Plasmodium falciparum sporozoites when administered one day pre-challenge and provided partial protection when administered seven days pre-challenge. Methods: A double-blind, randomized, placebo-controlled trial was performed to assess safety, tolerability, pharmacokinetics and efficacy of one oral dose of 400 mg DSM265 pre-CHMI...
December 5, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29203789/prevalence-of-malaria-in-two-highly-endemic-community-health-centers-in-the-bastar-district-chhattisgarh-showing-mixed-infections-with-plasmodium-species
#2
Sri Krishna, Ajay Yadav, Sneha Bhandari, Anup K Vishwakarma, Praveen K Bharti, Prem L Mandavi, Pradeep Bahgel, Sanjay Basak, Ravendra K Sharma, Neeru Singh
Malaria is a major public health problem in India and in the Chhattisgarh state. The diagnosis of malaria presents a major challenge in remote areas The prevalence of malaria in Darbha and Kilepal Community Health Centers (CHCs) of the Jagdalpur district, Chhattisgarh affected by conflict was determined using microscopy and polymerase chain reaction (PCR). In the year 2015, 29.4% and 21.5% cases were found to be positive for malaria at the Darbha and Kilepal CHCs, respectively, by microscopy, and 7.4% and 1...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29197418/tagging-to-endogenous-genes-of-plasmodium-falciparum-using-crispr-cas9
#3
Dexuan Kuang, Jichen Qiao, Zhou Li, Weiwei Wang, Hui Xia, Lubin Jiang, Jiejie Dai, Qiang Fang, Xueyu Dai
BACKGROUND: Plasmodium falciparum is the deadliest malaria parasite. Currently, there are seldom commercial antibodies against P. falciparum proteins, which greatly limits the study on Plasmodium. CRISPR/Cas9 is an efficient genome editing method, which has been employed in various organisms. However, the use of this technique in P. falciparum is still limited to gene knockout, site-specific mutation and generation of green fluorescent protein (GFP) reporter line with disruption of inserted sites...
December 2, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29184918/new-evidence-of-mating-swarms-of-the-malaria-vector-anopheles-arabiensis-in-tanzania
#4
Emmanuel W Kaindoa, Halfan S Ngowo, Alex Limwagu, Gustav Mkandawile, Japhet Kihonda, John Paliga Masalu, Hamis Bwanary, Abdoulaye Diabate, Fredros O Okumu
Background: Malaria mosquitoes form mating swarms around sunset, often at the same locations for months or years. Unfortunately, studies of Anopheles swarms are rare in East Africa, the last recorded field observations in Tanzania having been in 1983. Methods: Mosquito swarms were surveyed by trained volunteers between August-2016 and June-2017 in Ulanga district, Tanzania. Identified Anopheles swarms were sampled using sweep nets, and collected mosquitoes killed by refrigeration then identified by sex and taxa...
2017: Wellcome Open Research
https://www.readbyqxmd.com/read/29181142/molecular-screening-for-malaria-among-blood-donors-in-a-who-claimed-region-of-egypt-fayoum-governorate
#5
Salwa Bakr, Sherif Edris, Nashwa S Abdel Fattah, Noha Mohamed Ibrahim, Manal F El-Khadragy
Background: Transfusion-transmitted malaria is undoubtedly a potential health hazard for blood recipients. Egypt is still on the prevention of reintroduction phase of malaria control program. Fayoum Governorate is considered one of the high-risk foci in Egypt due to its geology. However, no studies have been reported to evaluate the current status of subclinical Plasmodium infection based on sensitive molecular techniques. Moreover, screening of malaria is not listed within screening protocols of blood-borne pathogens in Fayoum blood banks...
2017: Mediterranean Journal of Hematology and Infectious Diseases
https://www.readbyqxmd.com/read/29166928/effects-of-long-term-weekly-iron-and-folic-acid-supplementation-on-lower-genital-tract-infection-a-double-blind-randomised-controlled-trial-in-burkina-faso
#6
Loretta Brabin, Stephen A Roberts, Sabine Gies, Andrew Nelson, Salou Diallo, Christopher J Stewart, Adama Kazienga, Julia Birtles, Sayouba Ouedraogo, Yves Claeys, Halidou Tinto, Umberto d'Alessandro, E Brian Faragher, Bernard Brabin
BACKGROUND: Provision of routine iron supplements to prevent anaemia could increase the risk for lower genital tract infections as virulence of some pathogens depends on iron availability. This trial in Burkina Faso assessed whether weekly periconceptional iron supplementation increased the risk of lower genital tract infection in young non-pregnant and pregnant women. METHODS: Genital tract infections were assessed within a double blind, controlled, non-inferiority trial of malaria risk among nulliparous women, randomised to receive either iron and folic acid or folic acid alone, weekly, under direct observation for 18 months...
November 23, 2017: BMC Medicine
https://www.readbyqxmd.com/read/29162100/host-age-and-plasmodium-falciparum-multiclonality-are-associated-with-gametocyte-prevalence-a-1-year-prospective-cohort-study
#7
Yaw Adomako-Ankomah, Matthew S Chenoweth, Aaron M Tocker, Saibou Doumbia, Drissa Konate, Mory Doumbouya, Abdoul S Keita, Jennifer M Anderson, Rick M Fairhurst, Mahamadou Diakite, Kazutoyo Miura, Carole A Long
BACKGROUND: Since Plasmodium falciparum transmission relies exclusively on sexual-stage parasites, several malaria control strategies aim to disrupt this step of the life cycle. Thus, a better understanding of which individuals constitute the primary gametocyte reservoir within an endemic population, and the temporal dynamics of gametocyte carriage, especially in seasonal transmission settings, will not only support the effective implementation of current transmission control programmes, but also inform the design of more targeted strategies...
November 21, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29161482/-abundance-composition-and-natural-infection-of-anopheles-mosquitoes-from-two-malaria-endemic-regions-of-colombia
#8
Carolina Montoya, Priscila Bascuñán, Julián Rodríguez-Zabala, Margarita M Correa
INTRODUCTION: In Colombia there are three Anopheles species implicated in malaria transmission as primary vectors; however, the local role of some Anopheles species must still be defined. OBJECTIVE: To determine the abundance, composition and natural infection rates for Anopheles mosquitoes with Plasmodium spp. in two malaria-endemic regions of Colombia. MATERIALS AND METHODS: Anopheles mosquitoes were collected using the human-landing catches and while resting in livestock corrals in nine localities of two malaria-endemic regions of Colombia...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29152597/identification-of-spiroplasmainsolitum-symbionts-in-anopheles-gambiae
#9
Sharon T Chepkemoi, Enock Mararo, Hellen Butungi, Juan Paredes, Daniel Masiga, Steven P Sinkins, Jeremy K Herren
Background: Insect symbionts have the potential to block the transmission of vector-borne diseases by their hosts. The advancement of a symbiont-based transmission blocking strategy for malaria requires the identification and study of Anopheles symbionts. Methods: High throughput 16S amplicon sequencing was used to profile the bacteria associated with Anopheles gambiae sensu lato and identify potential symbionts. The polymerase chain reaction (PCR) with specific primers were subsequently used to monitor symbiont prevalence in field populations, as well as symbiont transmission patterns...
2017: Wellcome Open Research
https://www.readbyqxmd.com/read/29151024/insecticide-resistance-in-anopheles-arabiensis-from-ethiopia-2012-2016-a-nationwide-study-for-insecticide-resistance-monitoring
#10
Louisa A Messenger, Josephat Shililu, Seth R Irish, Gedeon Yohannes Anshebo, Alemayehu Getachew Tesfaye, Yemane Ye-Ebiyo, Sheleme Chibsa, Dereje Dengela, Gunawardena Dissanayake, Estifanos Kebede, Endalew Zemene, Abebe Asale, Mekonnen Yohannes, Hiwot Solomon Taffese, Kristen George, Christen Fornadel, Aklilu Seyoum, Robert A Wirtz, Delenasaw Yewhalaw
BACKGROUND: Indoor residual spraying (IRS) and long-lasting insecticidal nets (LLINs) remain the cornerstones of malaria vector control. However, the development of insecticide resistance and its implications for operational failure of preventative strategies are of concern. The aim of this study was to characterize insecticide resistance among Anopheles arabiensis populations in Ethiopia and describe temporal and spatial patterns of resistance between 2012 and 2016. METHODS: Between 2012 and 2016, resistance status of An...
November 18, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29145859/singapore-s-anopheles-sinensis-form-a-is-susceptible-to-plasmodium-vivax-isolates-from-the-western-thailand-myanmar-border
#11
Sook-Cheng Pang, Chiara Andolina, Benoit Malleret, Peter R Christensen, Sai-Gek Lam-Phua, Muhammad Aliff Bin Abdul Razak, Chee-Seng Chong, Daiqin Li, Cindy S Chu, Bruce Russell, Laurent Rénia, Lee-Ching Ng, Francois Nosten
BACKGROUND: Singapore has been certified malaria-free by the World Health Organization since November 1982. However, sporadic autochthonous malaria outbreaks do occur. In one of the most recent outbreaks of vivax malaria, an entomological investigation identified Anopheles sinensis as the most probable vector. As metaphase karyotype studies divided An. sinensis into two forms, A and B, with different vector competence: the investigation of vector competence of An. sinensis found in Singapore was thus pursued using Plasmodium vivax field isolates from the Thailand-Myanmar border...
November 16, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29143441/cellular-aging-dynamics-after-acute-malaria-infection-a-12-month-longitudinal-study
#12
Muhammad Asghar, Victor Yman, Manijeh Vafa Homann, Klara Sondén, Ulf Hammar, Dennis Hasselquist, Anna Färnert
Accelerated cellular aging and reduced lifespan have recently been shown in birds chronically infected with malaria parasites. Whether malaria infection also affects cellular aging in humans has not been reported. Here, we assessed the effect of a single acute Plasmodium falciparum malaria infection on cellular aging dynamics in travelers prospectively followed over one year in Sweden. DNA and RNA were extracted from venous blood collected at the time of admission and repeatedly up to one year. Telomere length was measured using real-time quantitative PCR, while telomerase activity and CDKN2A expression were measured by reverse transcriptase (RT)-qPCR...
November 16, 2017: Aging Cell
https://www.readbyqxmd.com/read/29141760/glucose-6-phosphate-dehydrogenase-deficiency-genetic-variants-in-malaria-patients-in-southwestern-ethiopia
#13
Tamar E Carter, Seleshi Kebede Mekonnen, Karen Lopez, Victoria Bonnell, Lambodhar Damodaran, Abraham Aseffa, Daniel A Janies
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked erythrocyte enzyme disorder with relevance to malaria treatment policy. Treatment with the antimalarial primaquine can result in hemolytic anemia in G6PD-deficient patients. With increased interest in primaquine use, it is important to identify G6PD variants in Ethiopia to inform malaria treatment policy. In the present study, mutations in the G6PD gene are identified in a sample of patients with malaria in Jimma town in southwest Ethiopia. Plasmodium species of infection were confirmed using polymerase chain reaction (PCR) and gel electrophoresis...
October 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29127650/-plasmodium-ovale-wallikeri-and-plasmodium-ovale-curtisi-malaria-in-senegal-in-2016
#14
M A Diallo, K Diongue, G Diagne, M C Seck, M Ndiaye, B Dièye, Y D Ndiaye, A S Badiane, D Ndiaye
Recently in Senegal, a case of Plasmodium ovale malaria had led to a diagnostic difficulty due to the ignorance of this parasite and the neglect of it. The objective of this study was to actively investigate cases of P. ovale malaria that would be misdiagnosed in the health centre structures of Senegal. The study was conducted in three areas that reflect different epidemiological strata of malaria. Microscopy was performed by microscopy experts on suspected malaria patients. The results were validated by Rougemont real-time PCR...
November 10, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/29127615/fungal-and-parasitic-cns-infections
#15
Pratibha Singhi, Arushi Gahlot Saini
Central nervous system fungal infections can be broadly divided into those that infect a healthy host such as Cryptococcus, Coccidioides, Histoplasma, Blastomyces, Sporothrix spp., and those that cause opportunistic infections in an immunocompromised host such as Candida, Aspergillus, Zygomycetes, Trichosporon spp. The clinical manifestations of central nervous system fungal infections commonly seen in children in clinical practice include a chronic meningitis or meningoencephalitis syndrome, brain abscess, rhino-cerebral syndrome and rarely, a fungal ventriculitis...
November 11, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/29125244/abundance-and-distribution-of-anopheles-mosquitoes-in-a-malaria-endemic-area-along-the-thai-lao-border
#16
Nattapol Marasri, Hans J Overgaard, Anchana Sumarnrote, Kanutcharee Thanispong, Vincent Corbel, Theeraphap Chareonviriyaphap
Malaria is an important public health problem in Thailand, especially along international borders. In this study, we conducted a longitudinal entomological survey in six villages and rubber plantation sites to address the spatio-temporal abundance and behavior of malaria vectors in Ubon Ratchathani Province along the Thailand-Laos border. Adult female mosquitoes were collected by human landing collections (indoor and outdoor) and by cattle bait collections twice per year, during rainy and dry seasons. Mosquitoes were morphologically identified and sibling species were determined by allele-specific PCR...
December 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/29125042/molecular-surveillance-of-chloroquine-drug-resistance-markers-pfcrt-and-pfmdr1-among-imported-plasmodium-falciparum-malaria-in-qatar
#17
Anushree Acharya, Devendra Bansal, Praveen K Bharti, Fahmi Y Khan, Salem Abusalah, Ashraf Elmalik, Mohammed ElKhalifa, Pradyumna K Mohapatra, Jagadish Mahanta, Rakesh Sehgal, Neeru Singh, Ali A Sultan
Imported malaria has been a great challenge for public health in Qatar due to influx of large number of migrant workers. Antimalarial drug resistance has emerged as one of the greatest challenges facing malaria control today. Monitoring parasite haplotypes that predict susceptibility to major antimalarial can guide treatment policies. This study aimed to determine molecular drug resistance pattern in imported malaria cases in Qatar. Blood samples from the uncomplicated P. falciparum malaria patients were collected at Hamad General Hospital, HMC, Doha, Qatar...
November 10, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/29121963/reassessment-of-asymptomatic-carriers-of-plasmodium-spp-in-an-endemic-area-with-a-very-low-incidence-of-malaria-in-extra-amazonian-brazil
#18
Filomena E C de Alencar, Rosely Dos Santos Malafronte, Crispim Cerutti, Lícia Natal Fernandes, Julyana Cerqueira Buery, Blima Fux, Helder Ricas Rezende, Angelica Espinosa Miranda
BACKGROUND: Regions with residual transmission are potential obstacles to the elimination of malaria. It is, therefore, essential to understand the factors associated with the maintenance of endemic malaria in these areas. The objective was to investigate whether the status of asymptomatic carriers of Plasmodium spp. DNA is maintained in the long term in an extra-Amazonian region of Brazil with low incidence, residual malaria transmission. METHODS: Asymptomatic carriers of Plasmodium DNA detected in a survey carried out between 2001 and 2004 were reassessed between 2010 and 2011 using questionnaires, PCR and thick and thin blood smear tests three times at 3-month intervals...
November 9, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29121931/individual-and-household-characteristics-of-persons-with-plasmodium-falciparum-malaria-in-sites-with-varying-endemicities-in-kinshasa-province-democratic-republic-of-the-congo
#19
Melchior Kashamuka Mwandagalirwa, Lauren Levitz, Kyaw L Thwai, Jonathan B Parr, Varun Goel, Mark Janko, Antoinette Tshefu, Michael Emch, Steven R Meshnick, Margaret Carrel
BACKGROUND: The Democratic Republic of the Congo (DRC) bears a large share of global malaria burden despite efforts to control and eliminate the disease. More detailed understanding of individual and household level characteristics associated with malaria are needed, as is an understanding of how these characteristics vary spatiotemporally and across different community-level malaria endemicities. An ongoing study in Kinshasa Province is designed to address gaps in prior malaria surveillance in the DRC by monitoring malaria across seasons, age groups and in high and low malaria sites...
November 9, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29119308/cryptic-species-anopheles-daciae-diptera-culicidae-found-in-the-czech-republic-and-slovakia
#20
Hana Blažejová, Oldřich Šebesta, František Rettich, Jan Mendel, Viktória Čabanová, Martina Miterpáková, Lenka Betášová, Juraj Peško, Zdeněk Hubálek, Helge Kampen, Ivo Rudolf
We report the distribution of mosquitoes of the maculipennis complex in two distinct areas of the Czech Republic (Bohemia and South Moravia) and in one locality of neighbouring Slovakia with emphasis on the detection of the newly described cryptic species Anopheles daciae (Linton, Nicolescu & Harbach, 2004). A total of 691 mosquitoes were analysed using a species-specific multiplex PCR assay to differentiate between the members of the maculipennis complex. In the Czech Republic, we found Anopheles maculipennis (with a prevalence rate of 1...
November 8, 2017: Parasitology Research
keyword
keyword
120160
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"