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https://www.readbyqxmd.com/read/28640824/molecular-diagnosis-of-plasmodium-ovale-by-photo-induced-electron-transfer-fluorogenic-primers-pet-pcr
#1
David Akerele, Dragan Ljolje, Eldin Talundzic, Venkatachalam Udhayakumar, Naomi W Lucchi
Accurate diagnosis of malaria infections continues to be challenging and elusive, especially in the detection of submicroscopic infections. Developing new malaria diagnostic tools that are sensitive enough to detect low-level infections, user friendly, cost effective and capable of performing large scale diagnosis, remains critical. We have designed novel self-quenching photo-induced electron transfer (PET) fluorogenic primers for the detection of P. ovale by real-time PCR. In our study, a total of 173 clinical samples, consisting of different malaria species, were utilized to test this novel PET-PCR primer...
2017: PloS One
https://www.readbyqxmd.com/read/28609010/submicroscopic-malaria-infections-in-pregnant-women-from-six-departments-in-haiti
#2
Maha A Elbadry, Massimiliano S Tagliamonte, Christian P Raccurt, Jean F Lemoine, Alexandre Existe, Jacques Boncy, Thomas A Weppelman, John B Dame, Bernard A Okech
OBJECTIVES: To describe the epidemiology of malaria in pregnancy in Haiti. METHODS: Cross-sectional study among pregnant women in 6 departments of Haiti. After obtaining informed consent, whole blood samples and demographic surveys were collected to investigate malaria prevalence, anemia and socio-behavioral risk factors for infection, respectively. 311 pregnant women were screened for Plasmodium falciparum infection using a rapid diagnostic test (RDT), microscopy, and a novel, quantitative reverse-transcriptase polymerase chain reaction method (qRT-PCR)...
June 13, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28604825/burden-and-impact-of-plasmodium-vivax-in-pregnancy-a-multi-centre-prospective-observational-study
#3
Azucena Bardají, Flor Ernestina Martínez-Espinosa, Myriam Arévalo-Herrera, Norma Padilla, Swati Kochar, Maria Ome-Kaius, Camila Bôtto-Menezes, María Eugenia Castellanos, Dhanpat Kumar Kochar, Sanjay Kumar Kochar, Inoni Betuela, Ivo Mueller, Stephen Rogerson, Chetan Chitnis, Dhiraj Hans, Michela Menegon, Carlo Severini, Hernando Del Portillo, Carlota Dobaño, Alfredo Mayor, Jaume Ordi, Mireia Piqueras, Sergi Sanz, Mats Wahlgren, Laurence Slutsker, Meghna Desai, Clara Menéndez
BACKGROUND: Despite that over 90 million pregnancies are at risk of Plasmodium vivax infection annually, little is known about the epidemiology and impact of the infection in pregnancy. METHODOLOGY AND PRINCIPAL FINDINGS: We undertook a health facility-based prospective observational study in pregnant women from Guatemala (GT), Colombia (CO), Brazil (BR), India (IN) and Papua New Guinea PNG). Malaria and anemia were determined during pregnancy and fetal outcomes assessed at delivery...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28591198/single-dose-primaquine-to-reduce-gametocyte-carriage-and-plasmodium-falciparum-transmission-in-cambodia-an-open-label-randomized-trial
#4
Jessica T Lin, Chanthap Lon, Michele D Spring, Somethy Sok, Soklyda Chann, Mali Ittiverakul, Worachet Kuntawunginn, Mok My, Kheangheng Thay, Rifat Rahman, Sujata Balasubramanian, Mengchuor Char, Charlotte A Lanteri, Panita Gosi, Ratawan Ubalee, Steven R Meshnick, David L Saunders
BACKGROUND: Single low dose primaquine (SLD PQ, 0.25mg/kg) is recommended in combination with artemisinin-based combination therapy (ACT) as a gametocytocide to prevent Plasmodium falciparum transmission in areas threatened by artemisinin resistance. To date, no randomized controlled trials have measured primaquine's effect on infectiousness to Anopheline mosquitoes in Southeast Asia. METHODS: Cambodian adults with uncomplicated falciparum malaria were randomized to receive a single 45mg dose of primaquine (equivalent to three SLD PQ) or no primaquine after the third dose of dihydroartemisin-piperaquine (DHP) therapy...
2017: PloS One
https://www.readbyqxmd.com/read/28460834/asymptomatic-plasmodial-infection-in-colombian-pregnant-women
#5
Jaime Carmona-Fonseca, Olga M Agudelo, Eliana M Arango
Information about asymptomatic plasmodial infection is scarce in the world, and the current antimalarial program goals (control, elimination, and eradication) demand this evidence to be well documented in different populations and malaria transmission settings. This study aimed to measure the prevalence of API in Colombian pregnant women at delivery. A retrospective prevalence survey was used. Women were recruited at hospital obstetric facility in each of the municipalities of Turbo, Necoclí in Antioquia department, and Puerto Libertador in Córdoba department...
April 28, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28427455/comparison-of-methods-for-detecting-asymptomatic-malaria-infections-in-the-china-myanmar-border-area
#6
COMPARATIVE STUDY
Yonghong Zhao, Yan Zhao, Yanmin Lv, Fei Liu, Qinghui Wang, Peipei Li, Zhenjun Zhao, Yingjie Liu, Liwang Cui, Qi Fan, Yaming Cao
BACKGROUND: Sensitive methods for detecting asymptomatic malaria infections are essential for identifying potential transmission reservoirs and obtaining an accurate assessment of malaria epidemiology in low-endemicity areas aiming to eliminate malaria. PCR techniques to detect parasite nucleic acids (DNA or RNA) are among the most commonly used molecular methods. However, most of these methods are of low throughput and cannot be used for large-scale molecular epidemiological studies...
April 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28420399/distinct-parasite-populations-infect-individuals-identified-through-passive-and-active-case-detection-in-a-region-of-declining-malaria-transmission-in-southern-zambia
#7
Kelly M Searle, Ben Katowa, Tamaki Kobayashi, Mwiche N S Siame, Sungano Mharakurwa, Giovanna Carpi, Douglas E Norris, Jennifer C Stevenson, Philip E Thuma, William J Moss
BACKGROUND: Substantial reductions in the burden of malaria have been documented in parts of sub-Saharan Africa, with elimination strategies and goals being formulated in some regions. Within this context, understanding the epidemiology of low-level malaria transmission is crucial to achieving and sustaining elimination. A 24 single-nucleotide-polymorphism Plasmodium falciparum molecular barcode was used to characterize parasite populations from infected individuals identified through passive and active case detection in an area approaching malaria elimination in southern Zambia...
April 19, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28363637/dsm265-for-plasmodium-falciparum-chemoprophylaxis-a-randomised-double-blinded-phase-1-trial-with-controlled-human-malaria-infection
#8
Mihály Sulyok, Thomas Rückle, Alexandra Roth, Raymund E Mürbeth, Stephan Chalon, Nicola Kerr, Sonia Schnieper Samec, Nathalie Gobeau, Carlos Lamsfus Calle, Javier Ibáñez, Zita Sulyok, Jana Held, Tamirat Gebru, Patricia Granados, Sina Brückner, Christian Nguetse, Juliana Mengue, Albert Lalremruata, B Kim Lee Sim, Stephen L Hoffman, Jörg J Möhrle, Peter G Kremsner, Benjamin Mordmüller
BACKGROUND: A drug for causal (ie, pre-erythrocytic) prophylaxis of Plasmodium falciparum malaria with prolonged activity would substantially advance malaria control. DSM265 is an experimental antimalarial that selectively inhibits the parasite dihydroorotate dehydrogenase. DSM265 shows in vitro activity against liver and blood stages of P falciparum. We assessed the prophylactic activity of DSM265 against controlled human malaria infection (CHMI). METHODS: At the Institute of Tropical Medicine, Eberhard Karls University (Tübingen, Germany), healthy, malaria-naive adults were allocated to receive 400 mg DSM265 or placebo either 1 day (cohort 1A) or 7 days (cohort 2) before CHMI by direct venous inoculation (DVI) of 3200 aseptic, purified, cryopreserved P falciparum sporozoites (PfSPZ Challenge; Sanaria Inc, Rockville, MD, USA)...
June 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28302168/comparison-of-the-diagnostic-performance-of-microscopic-examination-with-nested-polymerase-chain-reaction-for-optimum-malaria-diagnosis-in-upper-myanmar
#9
Jung-Mi Kang, Pyo-Yun Cho, Mya Moe, Jinyoung Lee, Hojong Jun, Hyeong-Woo Lee, Seong Kyu Ahn, Tae Im Kim, Jhang Ho Pak, Moe Kyaw Myint, Khin Lin, Tong-Soo Kim, Byoung-Kuk Na
BACKGROUND: Accurate diagnosis of Plasmodium infection is crucial for prompt malaria treatment and surveillance. Microscopic examination has been widely applied as the gold standard for malaria diagnosis in most part of malaria endemic areas, but its diagnostic value has been questioned, particularly in submicroscopic malaria. In this study, the diagnostic performance of microscopic examination and nested polymerase chain reaction (PCR) was evaluated to establish optimal malaria diagnosis method in Myanmar...
March 16, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28261219/naturally-acquired-binding-inhibitory-antibodies-to-plasmodium-vivax-duffy-binding-protein-in-pregnant-women-are-associated-with-higher-birth-weight-in-a-multicenter-study
#10
Pilar Requena, Myriam Arévalo-Herrera, Michela Menegon, Flor E Martínez-Espinosa, Norma Padilla, Camila Bôtto-Menezes, Adriana Malheiro, Dhiraj Hans, Maria Eugenia Castellanos, Leanne Robinson, Paula Samol, Swati Kochar, Sanjay K Kochar, Dhanpat K Kochar, Meghna Desai, Sergi Sanz, Llorenç Quintó, Alfredo Mayor, Stephen Rogerson, Ivo Mueller, Carlo Severini, Hernando A Del Portillo, Azucena Bardají, Chetan C Chitnis, Clara Menéndez, Carlota Dobaño
A vaccine to eliminate malaria would need a multi-stage and multi-species composition to achieve robust protection, but the lack of knowledge about antigen targets and mechanisms of protection precludes the development of fully efficacious malaria vaccines, especially for Plasmodium vivax (Pv). Pregnant women constitute a risk population who would greatly benefit from a vaccine preventing the adverse events of Plasmodium infection during gestation. We hypothesized that functional immune responses against putative targets of naturally acquired immunity to malaria and vaccine candidates will be associated with protection against malaria infection and/or poor outcomes during pregnancy...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28253867/the-shape-of-the-iceberg-quantification-of-submicroscopic-plasmodium-falciparum-and-plasmodium-vivax-parasitaemia-and-gametocytaemia-in-five-low-endemic-settings-in-ethiopia
#11
Fitsum G Tadesse, Lotus van den Hoogen, Kjerstin Lanke, Jodie Schildkraut, Kevin Tetteh, Abraham Aseffa, Hassen Mamo, Robert Sauerwein, Ingrid Felger, Chris Drakeley, Endalamaw Gadissa, Teun Bousema
BACKGROUND: The widespread presence of low-density asymptomatic infections with concurrent gametocytes may be a stumbling block for malaria elimination. This study investigated the asymptomatic reservoir of Plasmodium falciparum and Plasmodium vivax infections in schoolchildren from five settings in northwest Ethiopia. METHODS: Two cross-sectional surveys were conducted in June and November 2015, enrolling 551 students from five schools and 294 students from three schools, respectively...
March 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28219989/submicroscopic-falciparum-malaria-in-febrile-individuals-in-urban-and-rural-areas-of-gabon
#12
Denise P Mawili-Mboumba, Rosalie Nikiéma Ndong, Noemi Bahamontes Rosa, Jose Luis Llergo Largo, Aude Lembet-Mikolo, Prycil Nzamba, Christelle Offouga Mbouoronde, Maryvonne Kombila, Marielle K Bouyou Akotet
AbstractCharacterization of the parasite reservoir is required to improve malaria control. Asymptomatic patients with subpatent parasitemia have been identified in Gabon, but the prevalence of such infections among febrile subjects is unclear. We assessed the prevalence of submicroscopic Plasmodium falciparum infections on an island (Port-Gentil), and in urban (Libreville), semiurban (Melen), and rural (Oyem) settings in Gabon. Blood samples (N = 310) from febrile patients were tested for malaria parasites by quantitative nucleic acid sequence-based amplification (QT-NASBA)...
April 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28166813/hidden-reservoir-of-resistant-parasites-the-missing-link-in-the-elimination-of-falciparum-malaria
#13
Rashad Abdul-Ghani, Mohammed A K Mahdy, John C Beier, Leonardo K Basco
BACKGROUND: To successfully eliminate malaria, an integrated system that includes a number of approaches and interventions-aimed at overcoming the threat of antimalarial drug resistance-is required. Significant progress has been made in reducing malaria incidence through large-scale use of artemisinin-based combination therapies and insecticide-treated nets. To consolidate these gains, attention should be paid to the missing links in the elimination of malaria. One of these gaps is the residual reservoir of submicroscopic resistant parasites, which remains after case management or other control measures have been carried out...
February 6, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28143518/submicroscopic-plasmodium-prevalence-in-relation-to-malaria-incidence-in-20-villages-in-western-cambodia
#14
Rupam Tripura, Thomas J Peto, Christianne C Veugen, Chea Nguon, Chan Davoeung, Nicola James, Mehul Dhorda, Richard J Maude, Jureeporn Duanguppama, Krittaya Patumrat, Mallika Imwong, Lorenz von Seidlein, Martin P Grobusch, Nicholas J White, Arjen M Dondorp
BACKGROUND: Cambodia has seen a marked reduction in the incidence of Plasmodium falciparum over the past decade without a corresponding decline in Plasmodium vivax incidence. It is unknown to what extent local transmission is sustained by a chain of clinical and sub-clinical infections or by continued re-introduction via migration. Using an ultrasensitive molecular technique, 20 villages in western Cambodia were surveyed to detect the low season prevalence of P. falciparum and P. vivax and local treatment records were reviewed...
January 31, 2017: Malaria Journal
https://www.readbyqxmd.com/read/27938356/plasmodium-falciparum-genotype-and-gametocyte-prevalence-in-children-with-uncomplicated-malaria-in-coastal-ghana
#15
Ruth Ayanful-Torgby, Akua Oppong, Joana Abankwa, Festus Acquah, Kimberly C Williamson, Linda Eva Amoah
BACKGROUND: Plasmodium falciparum gametocytes are vital to sustaining malaria transmission. Parasite densities, multiplicity of infection as well as asexual genotype are features that have been found to influence gametocyte production. Measurements of the prevalence of Plasmodium sp. gametocytes may serve as a tool to monitor the success of malaria eradication efforts. METHODS: Whole blood was collected from 112 children aged between 6 months and 13 years with uncomplicated P...
December 9, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27893347/asymptomatic-infections-in-blood-donors-harbouring-plasmodium-an-invisible-risk-detected-by-molecular-and-serological-tools
#16
Giselle F M C Lima, Maria C Arroyo Sanchez, José E Levi, Mahyumi Fujimori, Luiza Da Cruz Caramelo, Arianni Rondelli Sanchez, Eduardo M Ramos-Sanchez, Juliana Inoue, Maria De Jesus Costa-Nascimento, Alfredo Mendrone Junior, Silvia M Di Santi
BACKGROUND: Transfusion-transmitted malaria due to asymptomatic Plasmodium infections is a challenge for blood banks. There is a lack of data on the prevalence of asymptomatic infected blood donors and the incidence of transfusion-transmitted malaria in low endemicity areas worldwide. We estimated the frequency of blood donors harbouring Plasmodium in an area in which asymptomatic infections have been reported. MATERIAL AND METHODS: To estimate the frequency of blood donors harbouring Plasmodium we used microscopy and molecular tools...
November 7, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27842542/asymptomatic-only-at-first-sight-malaria-infection-among-schoolchildren-in-highland-rwanda
#17
Kevin C Sifft, Dominik Geus, Caritas Mukampunga, Jean Claude Mugisha, Felix Habarugira, Kira Fraundorfer, Claude Bayingana, Jules Ndoli, Irenee Umulisa, Corine Karema, George von Samson-Himmelstjerna, Toni Aebischer, Peter Martus, Augustin Sendegeya, Jean Bosco Gahutu, Frank P Mockenhaupt
BACKGROUND: Plasmodium infection and malaria in school children are increasingly recognized as a relevant public health problem, but data on actual prevalence and health consequences are insufficient. The present study from highland southern Rwanda aimed at estimating infection prevalence among children attending school, at identifying associated factors and at assessing the clinical consequences of these infections. METHODS: In a survey including 12 schools in the Huye district of Rwanda, 1089 children aged 6-10 years were clinically and anthropometrically examined, malaria parasites were diagnosed by microscopy and PCR, haemoglobin concentrations were measured, and socio-economic and behavioural parameters as well as medical histories were obtained...
November 14, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27824866/a-field-tailored-reverse-transcription-loop-mediated-isothermal-assay-for-high-sensitivity-detection-of-plasmodium-falciparum-infections
#18
Sylvie Kemleu, Dylan Guelig, Carole Eboumbou Moukoko, Estelle Essangui, Steven Diesburg, Abas Mouliom, Bernard Melingui, Jeanne Manga, Christiane Donkeu, Annie Epote, Gaëtan Texier, Paul LaBarre, Robert Burton, Lawrence Ayong
Highly sensitive and field deployable molecular diagnostic tools are critically needed for detecting submicroscopic, yet transmissible levels of malaria parasites prevalent in malaria endemic countries worldwide. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and evaluated in comparison with thick blood smear microscopy, an antigen-based rapid diagnostic test (RDT), and an in-house RT-PCR targeting the same RT-LAMP transcript. The optimized assay detected Plasmodium falciparum infections in as little as 0...
2016: PloS One
https://www.readbyqxmd.com/read/27809907/high-prevalence-of-plasmodium-falciparum-gametocyte-infections-in-school-age-children-using-molecular-detection-patterns-and-predictors-of-risk-from-a-cross-sectional-study-in-southern-malawi
#19
Jenna E Coalson, Jenny A Walldorf, Lauren M Cohee, Miriam D Ismail, Don Mathanga, Regina Joice Cordy, Matthias Marti, Terrie E Taylor, Karl B Seydel, Miriam K Laufer, Mark L Wilson
BACKGROUND: In endemic areas, many people experience asymptomatic Plasmodium infections, particularly older children and adults, but their transmission contribution is unknown. Though not the exclusive determinant of infectiousness, transmission from humans to mosquitoes requires blood meals containing gametocytes. Gametocytes often occur at submicroscopic densities, challenging measurement in human populations. More sensitive molecular techniques allow better characterization of gametocyte epidemiologic patterns...
November 4, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27671736/plasmodium-falciparum-infection-in-febrile-congolese-children-prevalence-of-clinical-malaria-10-years-after-introduction-of-artemisinin-combination-therapies
#20
Mandingha Kosso Etoka-Beka, Francine Ntoumi, Michael Kombo, Julia Deibert, Pierre Poulain, Christevy Vouvoungui, Simon Charles Kobawila, Felix Koukouikila-Koussounda
OBJECTIVES: To investigate the proportion of malaria infection in febrile children consulting a paediatric hospital in Brazzaville, to determine the prevalence of submicroscopic malaria infection, to characterise Plasmodium falciparum infection and compare the prevalence of uncomplicated P. falciparum malaria according to haemoglobin profiles. METHODS: Blood samples were collected from children aged <10 years with an axillary temperature ≥37.5 °C consulting the paediatric ward of Marien Ngouabi Hospital in Brazzaville...
December 2016: Tropical Medicine & International Health: TM & IH
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