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https://www.readbyqxmd.com/read/29793204/influence-of-isolated-bacterial-strains-on-the-in-situ-biodegradation-of-endosulfan-and-the-reduction-of-endosulfan-contaminated-soil-toxicity
#1
Lingfen Kong, Yu Zhang, Lusheng Zhu, Jinhua Wang, Jun Wang, Zhongkun Du, Cheng Zhang
The recently discovered endosulfan-degrading bacterial strains Pusillimonas sp. JW2 and Bordetella petrii NS were isolated from endosulfan-polluted water and soil environments. The optimal conditions for the growth and biodegradation activity of the strains JW2 and NS were studied in detail. In addition, the ability of the strains JW2 and NS to biodegrade endosulfan in soils during in situ bioremediation experiments was investigated. At a concentration of 2 mg of endosulfan per kilogram of soil, both JW2 and NS had positive effects on the degradation of endosulfan; JW2 degraded 100% and 91...
May 21, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29793092/a-combined-ftir-and-dsc-study-on-the-bilayer-stabilising-effect-of-electrostatic-interactions-in-ion-paired-lipids
#2
Maximilian Schmid, Christian Wölk, Julia Giselbrecht, K L Andrew Chan, Richard D Harvey
Investigating lipid ion pair formation is important for understanding the mechanisms of lipid-mediated drug resistance in bacteria. In this study we have used the charged amphiphiles dipalmitoylphosphatidylglycerol (DPPG) and dihexadecyldimethylammonium bromide (DHDAB), as a model to evaluate the formation of ion pairs by a combined Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analysis. FTIR was employed to study the environment of the DPPC headgroup phosphate and lipid/surfactant alkane chains, in vesicles formed by the two amphiphiles mixed in various molar ratios...
May 17, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29793047/transcript-profiling-of-salt-tolerant-tobacco-mutants-generated-via-mutation-breeding
#3
Özge Çelik, Aybüke Ekşioğlu, Enes Yağız Akdaş
The main aim of the study is to identify the genes differentially, predominantly or specifically expressed in salt tolerant tobacco mutants, improved from Akhisar 97 and İzmir Özbaş varieties via mutation breeding, with respect to unstressed control plants. Seven tobacco mutants which have different salt tolerance capacities were evaluated by Gene Fishing analysis. Under stress conditions differentially expressed 100 reproducible bands were identified (74 of up-regulated and 20 of down-regulated while 6 were unknown)...
May 21, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29793044/isolation-of-ferritin-and-its-interaction-with-bmnpv-in-the-silkworm-bombyx-mori
#4
Dong-Qiong Fei, Hai-Zhong Yu, Jia-Ping Xu, Shang-Zhi Zhang, Jie Wang, Bing Li, Li-Ang Yang, Pei Hu, Xin Xu, Kang Zhao, Toufeeq Shahzad
Ferritin is a ubiquitous iron storage protein that plays an important role in host defence against pathogen infections. In the present study, native ferritin was isolated from the hemolymph of Bombyx mori using native-polyacrylamide gel electrophoresis (native-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that ferritin consisted of two subunits, designated as BmFerHCH and BmFerLCH. Previously integrated previous transcriptome and iTRAQ data showed that the two subunits were down-regulated in resistant silkworm strain BC9 and there was no obvious change in the expression levels of the subunits in susceptible silkworm strain P50 after BmNPV infection...
May 9, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29792943/the-development-of-a-screening-protocol-for-salmonella-spp-and-enteropathogenic-yersinia-spp-in-samples-from-wild-boar-sus-scrofa-also-generating-mlva-data-for-y-enterocolitica-and-y-pseudotuberculosis
#5
Axel Sannö, Magdalena Jacobson, Sandra Sterner, Susanne Thisted-Lambertz, Anna Aspán
Salmonellosis and yersiniosis are notifiable human diseases that are commonly associated with contaminated food. Domestic pigs as well as wild boars and other wild-life have been identified as reservoirs of these bacteria. Methods for cultivation and molecular epidemiological investigations of Salmonella spp. are well established, however, cultivation of enteropathogenic Yersinia spp. is time- consuming and the commonly used method for molecular epidemiological investigations, pulsed-field gel electrophoresis, lack in discriminatory power...
May 21, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29792779/association-of-colistin-resistant-kpc-clonal-strains-with-subsequent-infections-and-colonization-and-biofilm-production
#6
Melina Lorraine Ferreira, Bruna Fuga Araújo, Iara Rossi Gonçalves, Sabrina Royer, Paola Amaral Campos, Luiz Gustavo Machado, Deivid William Fonseca Batistão, Cristiane Silveira Brito, Paulo P Gontijo-Filho, Rosineide Marques Ribas
Carbapenemase-producing organisms are pandemic and a significant threat to public health. We investigated the clonal relatedness of colistin-resistant Klebsiella pneumoniae strains producing KPC-type carbapenemase (KPC-KP) causing subsequent infections or colonization. Moreover, we aimed to gain insight into the ability of biofilm production in K. pneumoniae strains producing carbapenemase. Twenty-two consecutive KPC-KP and one KPC-negative strain was identified from an adult intensive care unit in Brazil. Seventy-five percent of isolates that harbored the blaKPC gene exhibited genetic relatedness by pulsed-field gel electrophoresis, and none presented the plasmid-mediated mcr-1 and blaNDM genes...
May 24, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29792547/a-method-to-quantify-genotoxicity-of-malathion-in-rainbow-trout-using-the-weighted-averaging
#7
Hadi Poorbagher, Hamed Ghaffari Farsani, Hamid Farahmand
DNA breakage has been frequently used as a biomarker of the pesticide toxicity. The present study introduced a method to quantify the DNA breakage in Oncorhynchus mykiss exposed to the pesticide Malathion. Specimens were exposed to different concentrations of malathion for 1-9 days and their gill and liver were sampled. DNA was extracted and electrophoresed using agarose gel. The pixel density curves were obtained from the gel smears. The area under the curves were arbitrarily divided from 3 up to 7 segments using a Java macro in the software ImageJ...
May 24, 2018: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29792383/donor-variation-and-optimization-of-human-mesenchymal-stem-cells-chondrogenesis-in-hyaluronic-acid
#8
Minwook Kim, Isaac E Erickson, Alice H Huang, Sean T Garrity, Robert L Mauck, David R Steinberg
Mesenchymal stem cells (MSCs) are an attractive cell type for cartilage repair that can undergo chondrogenesis in a variety of three dimensional (3D) scaffolds. Hyaluronic acid (HA) hydrogels provide a biologically relevant interface for cell encapsulation. While our previous studies have shown that MSC-laden HA constructs can mature in vitro to match native mechanical properties using cells from animal sources, clinical application will depend on the successful translation of these findings to human cells...
May 24, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29792353/gastroretentive-raft-liquid-delivery-system-as-a-new-approach-to-release-extension-for-carrier-mediated-drug
#9
Samar M Abouelatta, Ahmed A Aboelwafa, Omaima N El-Gazayerly
Gabapentin (GBP), an antiepileptic and anti-neuropathic agent, suffers from short half-life (5-7 h), has narrow absorption window, and is absorbed via carrier-mediated mechanism resulting in frequent dosing, poor compliance, and poor bioavailability (<60%). Moreover, GBP is a freely water-soluble drug, thus it is considered a challenging candidate to be formulated as extended release dosage form. In this study, raft forming systems were investigated as a potential drug delivery system for prolonging gastric residence time of GBP...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29792233/antibiotic-resistance-and-molecular-characterization-of-diarrheagenic-escherichia-coli-and-non-typhoidal-salmonella-strains-isolated-from-infections-in-southwest-china
#10
Shun-Xian Zhang, Yong-Ming Zhou, Li-Guang Tian, Jia-Xu Chen, Rita Tinoco-Torres, Emmanuel Serrano, Shi-Zhu Li, Shao-Hong Chen, Lin Ai, Jun-Hu Chen, Shang Xia, Yan Lu, Shan Lv, Xue-Jiao Teng, Wen Xu, Wen-Peng Gu, Si-Tang Gong, Xiao-Nong Zhou, Lan-Lan Geng, Wei Hu
BACKGROUND: Bacterial diarrhea is one of the most common causes for medical consultations, mortality and morbidity in the world. Diarrheagenic Escherichia coli (DEC) and non-typhoidal Salmonella (NTS) are major intestinal pathogens in developing countries, and the indiscriminate use of antibiotics has greatly contributed to resistant strains. Hence, the aim of the present study is to identify the antimicrobial resistance patterns and the molecular characteristics of DEC and NTS in southwest, China...
May 7, 2018: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29791916/diclofenac-sodium-3-in-hyaluronic-acid-2-5-gel-significantly-diminishes-the-actinic-keratosis-area-and-severity-index
#11
Lutz Schmitz, Girish Gupta, Marc Hanno Segert, Ricarda Kost, Julia Sternberg, Thilo Gambichler, Eggert Stockfleth, Thomas Dirschka
BACKGROUND/AIMS: Actinic keratosis area and severity index (AKASI) is a new assessment tool to quantify the severity of actinic damage on the head. Thus far, it has not been evaluated in monitoring the efficacy of field-directed topical treatments in actinic keratosis (AK) in routine clinical practice. Thus, the aim of this study was to determine treatment outcomes by using AKASI 3 months after the initiation of topical application of diclofenac sodium 3% in hyaluronic acid 2.5% gel (DFS) in patients with AKs on the head...
May 23, 2018: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/29791784/chitosan-hydrogels-and-bionanocomposites-formed-through-the-mineralization-and-regulated-charging
#12
REVIEW
Irina Postnova, Vladimir Silant'ev, Sergei Sarin, Yury Shchipunov
The account presents survey of our systematic studies on chitosan. Only this polysaccharide bears cationic charges, possesses antimicrobial activity and wound healing ability that make it highly appropriate for using in medicine, biomedical engineering, cosmetics, food, packaging. However, its application meets with severe limitation. Chitosan belongs to polysaccharides that do not jellify solutions. Main approaches are based on the chemical modifications and cross-linking, but these treatments impairs therewith the biocompatibility and biological activity of chitosan...
May 23, 2018: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
https://www.readbyqxmd.com/read/29791489/contractile-force-measurement-of-human-induced-pluripotent-stem-cell-derived-cardiac-cell-sheet-tissue
#13
Daisuke Sasaki, Katsuhisa Matsuura, Hiroyoshi Seta, Yuji Haraguchi, Teruo Okano, Tatsuya Shimizu
We have developed our original tissue engineering technology "cell sheet engineering" utilizing temperature-responsive culture dishes. The cells are confluently grown on a temperature-responsive culture dish and can be harvested as a cell sheet by lowering temperature without enzymatic digestion. Cell sheets are high-cell-density tissues similar to actual living tissues, maintaining their structure and function. Based on this "cell sheet engineering", we are trying to create functional cardiac tissues from human induced pluripotent stem cells, for regenerative therapy and in vitro drug testing...
2018: PloS One
https://www.readbyqxmd.com/read/29791430/ex-vivos-models-to-teaching-therapeutic-endoscopic-ultrasound-t-eus
#14
Everson L A Artifon, Spencer Cheng, Thaisa Nakadomari, Leandro Kashiwagi, Jose Celso Ardengh, Emilio Belmonte, Jose P Otoch
BACKGROUND: Endoscopic ultrasound training has a learning curve greater than the other endoscopic therapeutic techniques. One of the preclinical teaching methods is the use of ex vivo porcine models. AIM: To describe five ex vivo porcine models for training in therapeutic echoendoscopic procedures. MATERIALS AND METHODS: Using porcine digestive tract containing esophagus, stomach, duodenum, spleen, liver and gallbladder, five models for therapeutic echoendoscopy training were described...
January 2018: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/29790733/mechanically-robust-gels-formed-from-hydrophobized-cellulose-nanocrystals
#15
Rinat Nigmatullin, Robert L Harniman, Valeria Gabrielli, Juan C Muñoz-Garcia, Yaroslav Z Khimyak, Jesús Angulo, Stephen J Eichhorn
Cellulose nanocrystals (CNCs) that bind to each other through associative hydrophobic interactions have been synthesized by modifying sulfated CNCs (sCNCs) with hydrophobic moieties. These octyl-CNCs form gels at significantly lower concentrations than parent sCNCs, producing extremely strong hydrogels. Unlike sCNCs, these octyl-CNCs do not form ordered liquid crystalline phases indicating a random association into a robust network driven by hydrophobic interactions. Furthermore, involvement of the octyl-CNCs into multicomponent supramolecular assembly was demonstrated in combination with starch...
May 23, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29790639/silylarene-hydrogenation-a-strategic-approach-enabling-direct-access-to-versatile-silylated-saturated-carbo-and-heterocycles
#16
Mario Wiesenfeldt, Tobias Knecht, Christoph Schlepphorst, Frank Glorius
Any[Si]ng but flat! We report a method to convert readily available silylated arenes into silylated saturated carbo- and heterocycles by arene hydrogenation. The scope includes alkoxy and halosilyl substituents. Silyl groups can be derivatized into a plethora of functionalities and find application in organic synthesis, materials science, pharmaceutical, agrochemical, and fragrance research. Yet, silylated saturated (hetero- ) cycles are difficult to access with current technologies. The yield of the hydrogenation depends on the amount of the silica gel additive...
May 23, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29790537/physical-defects-in-basement-membrane-mimicking-collagen-iv-matrices-trigger-cellular-emt-and-invasion
#17
Christopher Walter, Joshua T Davis, Jairaj Mathur, Amit Pathak
In fibrosis and cancer, degradation of basement membrane (BM) and cell invasion are considered as key outcomes of a cellular transformation called epithelial-mesenchymal transition (EMT). Here, we pose a converse question - can preexisting physical defects in the BM matrix cause EMT in normal epithelial cells? On a BM-mimicking matrix of collagen-IV-coated polyacrylamide (PA) gel, we have discovered a reverse phenomenon in which preexisting defects trigger EMT in normal epithelial cells. Through spatiotemporal measurements and simulations in silico, we demonstrate that the EMT precedes cellular mechanoactivation on defective matrices, but they occur concurrently on stiff matrices...
May 23, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29790526/food-grade-monoglyceride-oil-foams-the-effect-of-tempering-on-foamability-foam-stability-and-rheological-properties
#18
Robbe Heymans, Iris Tavernier, Sabine Danthine, Tom Rimaux, Paul Van der Meeren, Koen Dewettinck
Foams with a continuous oil phase may be stabilized using crystalline particles. Those systems are compelling because of their potential in edible oil structuring, modifying sensorial properties and creating healthier food products. This study aimed to relate oleogel (unwhipped state) properties to oil foam (whipped state) properties using a monoglyceride-sunflower oil model system. The properties of crystal-oil mixtures were influenced by time and temperature during preparation and storage. Therefore, oleogels were prepared using different tempering protocols and their resulting microstructure was investigated with rheology, differential scanning calorimetry and X-ray diffraction...
May 23, 2018: Food & Function
https://www.readbyqxmd.com/read/29790262/the-efficacy-and-tolerability-of-5-aminolevulinic-acid-5-thermosetting-gel-photodynamic-therapy-pdt-in-the-treatment-of-mild-to-moderate-acne-vulgaris-a-two-center-prospective-assessor-blinded-proof-of-concept-study
#19
Stefano Maria Serini, Maria Vittoria Cannizzaro, Annunziata Dattola, Virginia Garofalo, Esther Del Duca, Alessandra Ventura, Massimo Milani, Elena Campione, Luca Bianchi
BACKGROUND: Acne vulgaris is a chronic inflammatory skin disease, commonly treated with topical or systemic drugs, according to the severity of the condition. Retinoids and antibiotic compounds are considered cornerstone approaches in this condition. However, low adherence to the therapy and the issue of bacterial resistance undermine the efficacy in the long term. Photodynamic therapy (PDT) with 20% aminolevulinic acid (ALA) has shown to be effective in the treatment of inflammatory acne...
May 22, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29790054/removal-of-cobalt-and-lead-ions-from-wastewater-samples-using-an-insoluble-nanosponge-biopolymer-composite-adsorption-isotherm-kinetic-thermodynamic-and-regeneration-studies
#20
Anny Leudjo Taka, Elvis Fosso-Kankeu, Kriveshini Pillay, Xavier Yangkou Mbianda
In this study, an insoluble nanosponge biopolymer composite was synthesized, using a combined process of amidation reaction, cross-linking polymerization, and sol-gel method to obtain a phosphorylated multiwalled carbon nanotube-cyclodextrin/silver-doped titania (pMWCNT-βCD/TiO2 -Ag). This work mainly emphasized on the removal of lead (Pb2+ ) and cobalt (Co2+ ) metal ions from synthetic and real wastewater samples using the synthesized pMWCNT-βCD/TiO2 -Ag as a biosorbent. The new material was characterized by Fourier transform infrared (FTIR) spectroscopy, zeta potential, Brunauer-Emmett-Teller (BET) method, and scanning electron microscopy (SEM)...
May 23, 2018: Environmental Science and Pollution Research International
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