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https://www.readbyqxmd.com/read/28927258/a-proteomics-based-approach-reveals-differential-regulation-of-visceral-adipose-tissue-proteins-between-metabolically-healthy-and-unhealthy-obese-patients
#1
Assim A Alfadda, Afshan Masood, Mohammed Y Al-Naami, Pierre Chaurand, Hicham Benabdelkamel
Obesity and the metabolic disorders that constitute metabolic syndrome are a primary cause of morbidity and mortality in the world. Nonetheless, the changes in the proteins and the underlying molecular pathways involved in the relevant pathogenesis are poorly understood.In this study a proteomic analysis of the visceral adipose tissue isolated from metabolically healthy and unhealthy obese patients was used to identify presence of altered pathway(s) leading to metabolic dysfunction. Samples were obtained from 18 obese patients undergoing bariatric surgery and were subdivided into two groups based on the presence or absence of comorbidities as defined by the International Diabetes Federation...
September 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28927070/amentoflavone-enhances-sorafenib-induced-apoptosis-through-extrinsic-and-intrinsic-pathways-in-sorafenib-resistant-hepatocellular-carcinoma-sk-hep1-cells-in-vitro
#2
Wei-Lung Chen, Chia-Ling Hsieh, Jiann-Hwa Chen, Chih-Sheng Huang, Wei-Ting Chen, Yu-Cheng Kuo, Cheng-Yu Chen, Fei-Ting Hsu
The present study aimed to evaluate the effects of amentoflavone on sorafenib-induced apoptosis in sorafenib-resistant hepatocellular carcinoma (HCC) cells. The sorafenib-resistant SK-Hep1 (SK-Hep1R) cell line was established for the present study. Initially, the differences in sorafenib-induced cytotoxicity and apoptosis between wild-type SK-Hep1 and SK-Hep1R cells were verified using the MTT assay and flow cytometry. The effects of amentoflavone on sorafenib-induced cytotoxicity and apoptosis were then investigated using MTT, flow cytometry, DNA gel electrophoresis and western blot analysis...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926970/proteomic-characterization-of-armillaria-mellea-reveals-oxidative-stress-response-mechanisms-and-altered-secondary-metabolism-profiles
#3
Cassandra Collins, Rachel Hurley, Nada Almutlaqah, Grainne O'Keeffe, Thomas M Keane, David A Fitzpatrick, Rebecca A Owens
Armillaria mellea is a major plant pathogen. Yet, the strategies the organism uses to infect susceptible species, degrade lignocellulose and other plant material and protect itself against plant defences and its own glycodegradative arsenal are largely unknown. Here, we use a combination of gel and MS-based proteomics to profile A. mellea under conditions of oxidative stress and changes in growth matrix. 2-DE and LC-MS/MS were used to investigate the response of A. mellea to H₂O₂ and menadione/FeCl₃ exposure, respectively...
September 17, 2017: Microorganisms
https://www.readbyqxmd.com/read/28926713/phase-separation-kinetics-of-dynamically-self-assembling-nanoparticles-with-toggled-interactions
#4
Zachary M Sherman, Helen Rosenthal, James W Swan
Ordered materials passively self-assembled from dispersions of nanoparticles with steady interactions are subject to thermodynamic constraints on their phase separation kinetics forcing a tradeoff between throughput and quality. Dynamically self-assembling dispersions whose interactions vary in a controlled way with time do not have these constraints and can rapidly form ordered structures while avoiding kinetic arrest. These out-of-equilibrium processes cannot be understood in terms of equilibrium thermodynamics or kinetic models derived from equilibrium thermodynamics, so new theories must be developed before dynamic self-assembly can be used to reliably fabricate nanomaterials...
September 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28926708/molecular-nuances-governing-the-self-assembly-of-1-3-2-4-dibenzylidene-d-sorbitol
#5
Andrew Singh, France-Isabelle Auzanneau, Maria G Corradini, Girishma Grover, Richard G Weiss, Michael A Rogers
1,3:2,4 Dibenzylidene-D-sorbitol (DBS) is the gold-standard for low-molecular-weight organogelators (LMOGs). DBS gelates a wide array of solvents, as illustrated by the large Hansen sphere representing gels (2d = 33.5, p = 7.5, and h = 8.7 MPa1/2; radius = 11.2 MPa1/2). Derivatives of DBS have been synthesised to isolate and determine molecular features essential for organogelation. Herein, π-π stacking and hydrogen-bonding are the major non-covalent interactions examined. The importance of π-π stacking was studied using 1,3:2,4 dicyclohexanecarboxylidene-D-sorbitol (DCHS), which eliminates possible π-π stacking, while still conserving the other structural aspects of DBS...
September 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28926293/the-development-of-a-novel-wound-healing-material-silk-elastin-sponge
#6
Shingo Kawabata, Katsuya Kawai, Satoshi Somamoto, Kazuo Noda, Yoshitaka Matsuura, Yoko Nakamura, Shigehiko Suzuki
Silk-elastin is a recombinant protein polymer with repeating units of silk and elastin blocks. This novel wound healing promoting material has the ability to self-assemble from a liquid to a gel. We have already reported that an aqueous solution of silk-elastin has the potential to accelerate wound healing; however, there are several problems in applying silk-elastin in the clinical setting. To solve these problems, we developed a silk-elastin sponge that is easy to use in the clinical setting. In the present study, we examined whether the wound healing effect of the silk-elastin sponge is equal to the aqueous solution of silk-elastin in vivo...
September 19, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28926228/facile-synthesis-of-unique-cellulose-triacetate-based-flexible-and-high-performance-gel-polymer-electrolyte-for-lithium-ion-batteries
#7
Trupti Nirmale, Indrapal Karbhal, Ramchandra S Kalubarme, Manjusha V Shelke, Anjani J Varma, Bharat Bhanudas Kale
Lithium ion batteries (LIBs) with polymer based electrolytes have attracted huge attention due to the possibility of fabricating intrinsically safer and flexible devices. However, economical and eco-friendly sustainable technology is an oncoming challenge to fulfill the ever increasing demand. To circumvent this issue, we have developed gel polymer electrolyte (GPE) based on renewable polymers like cellulose triacetate (CTA) and poly (polyethylene glycol methacrylate) p(PEGMA) by using a photo polymerization technique...
September 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28926068/two-regions-of-microphase-separation-in-ion-containing-polymer-solutions
#8
Artem M Rumyantsev, Elena Yu Kramarenko
The phenomenon of spinodal decomposition in weakly charged polyelectrolyte solutions is studied theoretically within the random phase approximation. A novel feature of the theoretical approach is that it accounts for the effects of ionic association, i.e. ion pair and multiplet formation between counterions and ions in polymer chains, as well as the dependence of local dielectric permittivity on the polymer volume fraction Φ. The main focus is on the spinodal instability of polyelectrolyte solutions towards microscopic phase separation...
September 19, 2017: Soft Matter
https://www.readbyqxmd.com/read/28925975/new-insights-into-the-genome-organization-of-yeast-killer-viruses-based-on-atypical-killer-strains-characterized-by-high-throughput-sequencing
#9
Manuel Ramírez, Rocío Velázquez, Antonio López-Piñeiro, Belén Naranjo, Francisco Roig, Carlos Llorens
Viral M-dsRNAs encoding yeast killer toxins share similar genomic organization, but no overall sequence identity. The dsRNA full-length sequences of several known M-viruses either have yet to be completed, or they were shorter than estimated by agarose gel electrophoresis. High-throughput sequencing was used to analyze some M-dsRNAs previously sequenced by traditional techniques, and new dsRNAs from atypical killer strains of Saccharomyces cerevisiae and Torulaspora delbrueckii. All dsRNAs expected to be present in a given yeast strain were reliably detected and sequenced, and the previously-known sequences were confirmed...
September 19, 2017: Toxins
https://www.readbyqxmd.com/read/28925887/design-optimize-and-characterize-granisetron-hcl-loaded-nano-gel-for-transdermal-delivery
#10
Vikash Kumar, Hema Chaudhary, Geeta Aggarwal
The aim of study was to design, statistically optimize and characterize Granisetron HCl loaded transfersomal gel for transdermal delivery, in order to overcome the side effects associated with oral delivery. A response surface methodology experimental design was applied for the optimization of transfersomes, using Box-Behnken experimental design. Phospholipid-90G, sodium deoxycholate and sonication time, each at three levels, were selected as independent variables, and while entrapment efficiency, vesicle size and transdermal flux were identified as dependent variables...
September 15, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28925874/preclinical-assessment-of-steroidal-nanostructured-lipid-carriers-based-gels-for-atopic-dermatitis-optimization-and-product-development
#11
Upendra Nagaich, Neha Gulati
BACKGROUND: Betamethasone Valerate (BV) is potent topical corticosteroid. Preparation of nanostructured lipid carreirs (NLC) involves process parameters optimization and formulations were developed. It is available in several conventional formulations like creams and ointments which have well-known problems of frequent dosing and consequently additional side effects. The aim is to ascertain the probability of NLC as an exclusive carrier for betamethasone valerate topical application with regard to release modulation and improved therapeutic effect...
September 18, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/28925864/kinetic-characterization-of-anticarsia-gemmatalis-digestive-serine-proteases-and-the-inhibitory-effect-of-synthetic-peptides
#12
Adriana Patarroyo Vargas, Yaremis Meriño Cabrera, José Cola Zanuncio, Francelina Rocha, Wellington Garcia Campos, Maria Goreti de Almeida Oliveira
The kinetics of Anticarsia gemmatalis Hübner gut serine proteases with tripeptidyl substrates, and the enzymatic inhibition model using synthetic peptides Gor 3, Gor 4, and Gor 5 are described. The enzymes were purified on p-aminobenzamidine agarose affinity column in an FPLC system using electrophoresis with 12.5% polyacrylamide gel. Michaelis-Menten constants and the inhibition model were determined according to the Dixon methodology and Lineweaver-Burk's double reciprocal. The KM values and catalytic constants of peptide substrates show that A...
September 17, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28925581/advanced-materials-based-on-polymers-and-ionic-liquids
#13
REVIEW
Yuzo Kitazawa, Kazuhide Ueno, Masayoshi Watanabe
Ionic liquids (ILs) are ambient temperature molten salts, which have attracted considerable attention owing to their unique properties. In this contribution, we review advanced materials composed of ILs and polymers for the basis of a new design protocol to fabricate novel materials. As electrolytes for electrochemical devices, cross-linked polymers containing ILs (ion gels) are endowed with functional properties inherited from ILs and mechanical consistency derived from polymers. To create such materials, micro-phase separation of block copolymers and colloidal arrays in the ILs are utilized...
September 19, 2017: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
https://www.readbyqxmd.com/read/28925517/differences-in-protein-expression-among-five-species-of-stream-stonefly-plecoptera-along-a-latitudinal-gradient-in-japan
#14
Maribet Gamboa, Maria Claret Tsuchiya, Suguru Matsumoto, Hisato Iwata, Kozo Watanabe
Proteome variation among natural populations along an environmental gradient may provide insights into how the biological functions of species are related to their local adaptation. We investigated protein expression in five stream stonefly species from four geographic regions along a latitudinal gradient in Japan with varying climatic conditions. The extracted proteins were separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization of time-of-flight (MALDI TOF/TOF), yielding 446 proteins...
September 19, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28925158/-study-on-chemical-constituents-of-seeds-of-croton-tiglium-and-their-cytotoxicities
#15
Hai-Guo Su, Huai Yang, Chun-Wang Meng, Cheng Peng, Li Guo, Ou Dai
Seven compounds were isolated from the seeds of Croton tiglium by preparative TLC, semi-preparative HPLC, and column chromatography over silica gel, MCI, and Sephadex LH-20, etc. Their structures were elucidated by spectroscopic data analysis as bis(2,3-dihydroxypropyl) nonanedioate (1), 12-O-(α-methyl)butyrylphorbol-13-decanoate (2), 12-O-tiglylphorbol-13-decanoate (3), (9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoic acid (4), methyl (9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoate (5), 4(1H)-quinolinone (6), and 5-hydroxy-2-pyridinemethanol (7)...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925155/-analysis-on-dna-methylation-of-mosaic-and-moxa-type-leaves-of-aconitum-carmichaeli-by-msap
#16
Huan Wen, Cheng Peng, Chao-Long Rao, Da-Yan Zhang, Xiao-Fang Xie, Ji-Hai Gao
An MSAP analysis method was established for detecting DNA methylation of Aconitum carmichaeli leaves, and the DNA methylation of different leaf shapes and different leaf position was analyzed by MSAP. The study made experiments on the leaves of different position of mosaic and moxa leaf type A. carmichaeli, researched the effects of restriction digestion of genomic DNA by using two restriction enzymes, screened the suitable selective amplification primers, and analyzed the methylation differences of leaves by calculating the 6% acrylamide gel electrophoresis bands and lane...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28924947/development-of-a-rapid-maldi-tof-ms-based-epidemiological-screening-method-using-mrsa-as-a-model-organism
#17
Åsa Lindgren, Nahid Karami, Roger Karlsson, Christina Åhrén, Martin Welker, Edward R B Moore, Liselott Svensson Stadler
In this study we present a method using whole cell MALDI-TOF MS and VITEK MS RUO/SARAMIS as a rapid epidemiological screening tool. MRSA was used as a model organism for setting up the screening strategy. A collection of well-characterised MRSA strains representing the 19 most common Pulsed-Field Gel Electrophoresis (PFGE)-types in the region of South-West Sweden for the past 20 years was analysed with MALDI-TOF MS. A total of 111 MRSA strains were used for creating 19 PFGE-specific Superspectra using VITEK MS RUO/SARAMIS...
September 18, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28924458/control-of-nuclear-cytoplasmic-shuttling-of-ankrd54-by-pkc%C3%AE
#18
Amy L Samuels, Alison Louw, Reza Zareie, Evan Ingley
AIM: To identify and characterize the effect of phosphorylation on the subcellular localization of Ankrd54. METHODS: HEK293T cells were treated with calyculin A, staurosporin or phorbol 12-myristate 13-acetate (PMA). Cells were transfected with eGFP-tagged Ankrd54 with or without Lyn tyrosine kinase (wild-type, Y397F mutant, or Y508F mutant). The subcellular localization was assessed by immunofluorescence imaging of cells, immunoblotting of subcellular fractionations...
August 26, 2017: World Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28924029/circuitry-linking-the-global-csr-and-%C3%AF-e-dependent-cell-envelope-stress-response-systems
#19
Helen Yakhnin, Robert Aichele, Sarah E Ades, Tony Romeo, Paul Babitzke
CsrA of Escherichia coli is an RNA-binding protein that globally regulates a wide variety of cellular processes and behaviors including carbon metabolism, motility, biofilm formation, and the stringent response. CsrB and CsrC are sRNAs that sequester CsrA, thereby preventing CsrA-mRNA interaction. RpoE (σ(E)) is the extracytoplasmic stress response sigma factor of E. coli Previous RNA-seq studies identified rpoE mRNA as a CsrA target. Here we explored the regulation of rpoE by CsrA and found that CsrA represses rpoE translation...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28923775/immunoadsorption-using-immusorba-tr-and-ph
#20
REVIEW
Ryuichiro Hirano, Noriko Hirata
Immusorba TR (IM-TR) and PH (IM-PH) were developed as immunoadsorbents from nonbiological materials as affinity ligands for removal of pathogenic substances. The immunoadsorbents in IM-TR and IM-PH are immobilized on a polyvinyl alcohol gel with tryptophan and phenylalanine, respectively, as a ligand. IM-TR is mainly clinically applied to autoimmune neurological diseases such as myasthenia gravis, Guillain-Barré syndrome, and multiple sclerosis. IM-PH is also applied to neurological diseases but mainly to rheumatic diseases such as rheumatoid arthritis and systemic lupus erythematosus...
August 30, 2017: Transfusion and Apheresis Science
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