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https://www.readbyqxmd.com/read/28445861/the-application-of-silicon-sol-gel-technology-to-forensic-blood-substitute-development-investigation-of-the-spreading-dynamics-onto-a-paper-surface
#1
Theresa Stotesbury, Mike Illes, Paul Wilson, Andrew J Vreugdenhil
This work investigates the spreading dynamics of three candidate sol-gel solutions, of ranging viscosities, surface tensions and densities, and compares them with water and two commercial blood substitute products. Droplets were created with different sizes (10 to75μL) and impact velocities (1.4 to 6.0m/s) to strike 176gsm cardstock. Over 2200 droplets were created using the six different fluids and their final dried stain diameter was measured. Droplet spread was plotted using the Scheller and Bousfield correlation and uses effective viscosity as a parameter for non-Newtonian fluids...
April 1, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28445758/bilayer-interactions-among-unsaturated-phospholipids-sterols-and-ceramide
#2
J Peter Slotte, Tomokazu Yasuda, Oskar Engberg, Md Abdullah Al Sazzad, Victor Hautala, Thomas K M Nyholm, Michio Murata
Using differential scanning calorimetry and lifetime analysis of trans-parinaric acid fluorescence, we have examined how cholesterol and cholesteryl phosphocholine (CholPC) affect gel-phase properties of palmitoyl ceramide (PCer) in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleyol-sn-glycero-3-phosphocholine (DOPC) bilayers. By (2)H NMR, we also measured fluid-phase interactions among these lipids using deuterated analogs of POPC, PCer, and cholesterol. The PCer-rich gel phase in POPC bilayers (9:1 molar ratio of POPC to PCer) was partially and similarly dissolved (and thermostability decreased) by both cholesterol and CholPC (sterol was present equimolar to PCer, or in fourfold excess)...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28445616/extract-of-syzygium-aromaticum-suppress-eef1a-protein-expression-and-fungal-growth
#3
Yihe Wang, Yiran Ding, Shunhe Wang, Haiqin Chen, Zhang Hao, Wei Chen, Zhennan Gu, Yong Q Chen
AIMS: Clove extract has therapeutic potential as an antifungal drug, yet the mechanism of action remain ambiguous. Current study aimed to address the molecular process of the antifungal activity exerted by clove extract. METHODS AND RESULTS: The antifungal assays results showed that clove extract had some effects on all of the tested yeast. Propidium iodide staining assay showed cell membrane damage in Saccharomyces cerevisiae after treatment of clove extract for 30 h...
April 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28445518/use-of-human-fat-grafting-in-the-prevention-of-perineural-adherence-experimental-study-in-athymic-mouse
#4
Mario Cherubino, Igor Pellegatta, Alessandro Crosio, Luigi Valdatta, Stefano Geuna, Rosalba Gornati, Pierluigi Tos
Perineural adherences represent a problem after surgery involving peripheral neural system. Fat-grafting with adipose derived stem cells (ASCs) with their pro-regenerative characteristics can be important to prevent the neural damage or to facilitate the neural regeneration. Our idea was to use the fat-grafting as an anti-adherence device and test its efficacy on a postsurgical scar animal model and comparing to an antiadhesive gel. 32 athymic mice were operated under magnification, we exposed both sciatic nerves...
2017: PloS One
https://www.readbyqxmd.com/read/28445456/floor-plate-derived-netrin-1-is-dispensable-for-commissural-axon-guidance
#5
Chloé Dominici, Juan Antonio Moreno-Bravo, Sergi Roig Puiggros, Quentin Rappeneau, Nicolas Rama, Pauline Vieugue, Agns Bernet, Patrick Mehlen, Alain Chédotal
Netrin-1 is an evolutionarily conserved, secreted extracellular matrix protein involved in axon guidance at the central nervous system midline. Netrin-1 is expressed by cells localized at the central nervous system midline, such as those of the floor plate in vertebrate embryos. Growth cone turning assays and three-dimensional gel diffusion assays have shown that netrin-1 can attract commissural axons. Loss-of-function experiments further demonstrated that commissural axon extension to the midline is severely impaired in the absence of netrin-1 (refs 3, 7, 8, 9)...
April 26, 2017: Nature
https://www.readbyqxmd.com/read/28445050/involvement-of-wrky-transcription-factors-in-aba-induced-cold-tolerance-of-banana-fruit
#6
Dong-Lan Luo, Liang-Jie Ba, Wei Shan, Jian-Fei Kuang, Wang-Jin Lu, Jian-Ye Chen
Phytohormone abscisic acid (ABA) and plant-specific WRKY transcription factors (TFs) have been implicated to play important roles in various stress responses. The involvement of WRKY TFs in ABA-mediated cold tolerance of economical fruits, such as banana fruit, however remains largely unknown. Here, we reported that ABA application could induce expressions of ABA biosynthesis-related genes MaNCED1 and MaNCED2, increase endogenous ABA contents and thereby enhance cold tolerance in banana fruit. Four banana fruit WRKY TFs, designated as MaWRKY31, MaWRKY33, MaWRKY60, and MaWRKY71, were identified and characterized...
April 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28444798/multi-gel-casting-apparatus-for-vertical-polyacrylamide-gels-with-in-built-solution-flow-system-and-liquid-level-detectors
#7
Praveen Maurye, Arpita Basu, Tapas Kumar Bandyopadhyay, Jayanta Kumar Biswas, Bimal Prasana Mohanty
Polyacrylamide gel electrophoresis (PAGE) is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and function of cells. Simultaneous analysis of multiple protein samples often requires casting of many PAGE gels. Several variants of multi-gel casting/electrophoresis apparatuses are frequently used in research laboratories...
April 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28444689/the-young-s-modulus-fracture-stress-and-fracture-strain-of-gellan-hydrogels-filled-with-whey-protein-microparticles
#8
Cherry Wing Yu Lam, Shinya Ikeda
Texture modifying abilities of whey protein microparticles are expected to be dependent on pH during heat-induced aggregation of whey protein in the microparticulation process. Therefore, whey protein microparticles were prepared at either pH 5.5 or 6.8 and their effects on small and large deformation properties of gellan gels containing whey protein microparticles as fillers were investigated. The majority of whey protein microparticles had diameters around 2 μm. Atomic force microscopy images showed that whey protein microparticles prepared at pH 6...
April 25, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28443937/supramolecular-materials
#9
David B Amabilino, David K Smith, Jonathan W Steed
Molecular material properties depend upon the contacts between and the arrangement of the component parts, and therefore supramolecular chemistry has developed a highly important role in this area. This Tutorial Review, after briefly introducing the history of the field, discusses some of the most exciting and inspiring recent achievements, with special focus on soft materials, particularly gels and liquid crystals.
April 26, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28443926/defect-induced-ferromagnetism-in-mgo-and-its-exceptional-enhancement-upon-thermal-annealing-a-case-of-transformation-of-various-defect-states
#10
Nimai Pathak, Santosh Kumar Gupta, C L Prajapat, S K Sharma, P S Ghosh, Buddhadev Kanrar, P K Pujari, R M Kadam
MgO particles of few micron size are synthesized through a sol-gel method at different annealing temperatures such as 600 °C (MgO-600), 800 °C (MgO-800) and 1000 °C (MgO-1000). EDX and ICP-AES studies confirmed a near total purity of the sample with respect to paramagnetic metal ion impurities. Magnetic measurements showed a low temperature weak ferromagnetic ordering with a TC (Curie temperature) around 65 K (±5 K). Unexpectedly, the saturation magnetization (Ms) was found to be increased with increasing annealing temperature during synthesis...
April 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28443922/friction-lubrication-and-in-situ-mechanics-of-poroelastic-cellulose-hydrogels
#11
G K Dolan, G E Yakubov, M R Bonilla, P Lopez-Sanchez, J R Stokes
The tribology between biphasic materials is challenging to predict and interpret due to the interrelationship between mechanical properties, microstructure and movement of the fluid phase contained within. A new approach is presented to deconvolute these effects for cellulose hydrogels, which have a fibrous network that is akin to the microstructure of articular cartilage and plant cell walls. This is achieved by developing a tribo-rheological technique that uniquely incorporates in situ mechanical characterisation (compression-relaxation and small amplitude oscillatory shear) immediately prior to measuring the tribological response between pairs of hydrogels...
April 26, 2017: Soft Matter
https://www.readbyqxmd.com/read/28443918/ph-responsiveness-of-hydrogels-formed-by-telechelic-polyampholytes
#12
Margarita A Dyakonova, George Gotzamanis, Bart-Jan Niebuur, Natalya S Vishnevetskaya, Konstantinos N Raftopoulos, Zhenyu Di, Sergey K Filippov, Constantinos Tsitsilianis, Christine M Papadakis
We investigate the influence of pH on the rheological and structural properties of hydrogels formed by hydrophobic association of the sticky ends of the triblock terpolymer poly(methyl methacrylate)-b-poly(2-(diethylamino)ethyl methacrylate-co-methacrylic acid)-b-poly(methyl methacrylate) (PMMA-b-P(DEA-co-MAA)-b-PMMA). The middle block is a weak polyampholyte having a pH dependent charge density and sign, which enables tuning of the rheological and structural properties by pH variation. Small-angle neutron scattering (SANS) studies of solutions in D2O at 0...
April 26, 2017: Soft Matter
https://www.readbyqxmd.com/read/28443909/effect-of-the-calcination-temperature-on-the-photocatalytic-efficiency-of-acidic-sol-gel-synthesized-tio2-nanoparticles-in-the-degradation-of-alprazolam
#13
Andreia Romeiro, Diana Freitas, M Emília Azenha, Moisés Canle, Hugh D Burrows
We report a comparative study on the photodegradation of the widely used benzodiazepine psychoactive drug alprazolam (8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine, ALP) using direct photolysis, and titanium dioxide photocatalyzed reaction. Titanium dioxide photocatalysts were prepared as nanoparticles by acidic sol-gel methods, calcined at two different temperatures, and their behavior compared with P25 (Degussa type) TiO2. Efficient photodegradation was observed in the photocatalytic process, with over 90% degradation after 90 minutes under optimized conditions...
April 26, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28443902/an-internal-magnetic-field-strategy-to-reuse-pulverized-active-materials-for-high-performance-a-magnetic-three-dimensional-ordered-macroporous-tio2-copt-%C3%AE-fe2o3-nanocomposite-anode
#14
Yiping Tang, Liang Hong, Jiquan Li, Guangya Hou, Huazhen Cao, Liankui Wu, Guoqu Zheng, Qingliu Wu
A ferromagnetic three-dimensional ordered macroporous TiO2/CoPt/α-Fe2O3 (3DOMTCF) nanocomposite was synthesized via a sol-gel approach templated by poly(methyl methacrylate) (PMMA) microspheres. After magnetization, it exhibited an extremely high reversible capacity and a long cycle life, which were ascribed to the internal magnetic field for reusing pulverized active materials and its unique structure.
April 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28443878/influence-of-high-energy-electron-irradiation-on-the-network-structure-of-gelatin-hydrogels-as-investigated-by-small-angle-x-ray-scattering-saxs
#15
Emilia I Wisotzki, Paolo Tempesti, Emiliano Fratini, Stefan G Mayr
The impact of high energy crosslinking on the network structure of gelatin hydrogels was investigated in comparison to physically entangled gels by small-angle X-ray scattering (SAXS). Physically entangled gelatin of increasing concentration exhibited a nearly constant correlation length of several nanometers. These gels had scattering behavior close to that of polymer coils swollen in a good solvent, as evidenced by the Porod exponent of 1.8. The mass fractal dimension decreased towards 1, indicating increased formation of semiflexible gelatin triple helices and rod-like structures as a function of the gelatin concentration...
April 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28443870/operando-x-ray-spectroscopic-observations-of-modulations-of-local-atomic-and-electronic-structures-of-color-switching-smart-film
#16
Ying-Rui Lu, Tzung-Zing Wu, Han-Wei Chang, Jeng-Lung Chen, Chi-Liang Chen, Da-Hau Wei, Jin-Ming Chen, Wu-Ching Chou, Chung-Li Dong
Smart windows, which change color in response to external stimuli, are extensively studied owing to their potential technological applications in sensors and their ability to reduce the energy consumed by buildings. Most related studies focus on the optical properties of smart color switching films that can control the transmission of light and that of heat independently. This study examines the vanadium pentoxide thin film as a model system of a color switchable window. A gasochromic thin film of V2O5 is fabricated using sol-gel spin coating...
April 26, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28443326/ionic-liquid-silica-precursors-via-solvent-free-sol-gel-process-and-their-application-in-epoxy-amine-network-a-theoretical-experimental-study
#17
Magdalena Perchacz, Ricardo Keitel Donato, Leandro Seixas, Alexander Zhigunov, Rafal Konefal, Magdalena Serkis-Rodzeń, Hynek Benes
This work describes the solvent-free sol-gel synthesis of epoxy-functionalized silica-based precursors, in the presence of 1-butyl-3-methylimidazolium-based ionic liquids (ILs) containing different anions - chloride (Cl(-)) and methanesulfonate (MeSO3(-)). The IL-driven sol-gel mechanisms were investigated in details using experimental characterizations ((29)Si NMR and ATR FTIR spectroscopy) and a theoretical computational method based on density functional theory (DFT). We observed a complex IL's influence on both hydrolysis and condensation steps, involving especially H-bonding and Coulomb coupling stabilization of the process intermediates...
April 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28443290/comparison-of-a-supraglottic-airway-device-v-gel-%C3%A2-with-blind-orotracheal-intubation-in-rabbits
#18
Sarah Engbers, Amy Larkin, Nicolas Rousset, Melanie Prebble, Mahesh Jonnalagadda, Cameron G Knight, Daniel S J Pang
INTRODUCTION: Achieving a secure airway in rabbits is generally considered more difficult than in cats or dogs. Their relatively large tongue, small oropharyngeal cavity and glottis limit direct visualization. A rabbit-specific supraglottic airway device (SGAD) may offer benefits over blind orotracheal intubation. ANIMALS AND METHODS: Fifteen adult New Zealand white rabbits were randomized to SGAD or orotracheal intubation (ETT). All animals were sedated with dexmedetomidine (0...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28443116/comparative-analysis-of-phosphoproteome-remodeling-after-short-term-water-stress-and-aba-treatments-versus-longer-term-water-stress-acclimation
#19
Govinal B Bhaskara, Thao T Nguyen, Tsu-Hao Yang, Paul E Verslues
Several studies have used short term dehydration, osmotic stress or Abscisic Acid (ABA) treatments to identify the initial protein phosphorylation-dephosphorylation responses to drought and low water potential or ABA treatments. However, longer term drought acclimation leads to altered expression of many kinases and phosphatases suggesting that it may also produce unique changes in phosphoproteome composition. To get a better overview of the state of drought-related phosphoproteomics and investigate this question of short versus longer term phosphoproteome regulation, we compared three Arabidopsis thaliana studies analyzing short term phosphoproteome changes to recent data from our laboratory analyzing phosphoproteome changes after a longer drought acclimation treatment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28443113/barcode-system-for-genetic-identification-of-soybean-glycine-max-l-merrill-cultivars-using-indel-markers-specific-to-dense-variation-blocks
#20
Hwang-Bae Sohn, Su-Jeong Kim, Tae-Young Hwang, Hyang-Mi Park, Yu-Young Lee, Kesavan Markkandan, Dongwoo Lee, Sunghoon Lee, Su-Young Hong, Yun-Ho Song, Bon-Cheol Koo, Yul-Ho Kim
For genetic identification of soybean [Glycine max (L.) Merrill] cultivars, insertions/deletions (InDel) markers have been preferred currently because they are easy to use, co-dominant and relatively abundant. Despite their biological importance, the investigation of InDels with proven quality and reproducibility has been limited. In this study, we described soybean barcode system approach based on InDel makers, each of which is specific to a dense variation block (dVB) with non-random recombination due to many variations...
2017: Frontiers in Plant Science
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