keyword
MENU ▼
Read by QxMD icon Read
search

Gel

keyword
https://www.readbyqxmd.com/read/29156646/inhibition-of-bcl-2-and-cox-2-protein-expression-after-local-application-of-a-new-carmustine-loaded-clinoptilolite-based-delivery-system-in-a-chemically-induced-skin-cancer-model-in-mice
#1
Cristina Mihaela Ghiciuc, Aurel Lulu Strat, Lacramioara Ochiuz, Catalina Elena Lupusoru, Maria Ignat, Aurelia Vasile, Alexandru Grigorovici, Iulian Stoleriu, Carmen Solcan
Our research has focused on in vitro and in vivo evaluations of a new Carmustine (BCNU)-loaded clinoptilolite-based delivery system. Two clinoptilolite ionic forms-hydrogen form (HCLI) and sodium form (NaCLI)-were prepared, allowing a loading degree of about 5-6 mg BCNU/g of zeolite matrix due to the dual porous feature of clinoptilolite. Clinoptilolite-based delivery systems released 35.23% of the load in 12 h for the BCNU@HCLI system and only 10.82% for the BCNU@NaCLI system. The BCNU@HCLI system was chosen to develop gel and cream semisolid dosage forms...
November 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29156636/structural-electrical-magnetic-and-resistive-switching-properties-of-the-multiferroic-ferroelectric-bilayer-thin-films
#2
Ming-Cheng Kao, Hone-Zern Chen, San-Lin Young, Kai-Huang Chen, Jung-Lung Chiang, Jen-Bin Shi
Bi0.8Pr0.2Fe0.95Mn0.05O₃/Bi3.96Gd0.04Ti2.95W0.05O12 (BPFMO/BGTWO) bilayer thin films with Multiferroic/Ferroelectric (MF/FE) structures were deposited onto Pt(111)/Ti/SiO₂/Si(100) substrates by using the sol-gel method with rapid thermal annealing. The BPFMO/BGTWO thin films exhibited well-saturated ferromagnetic and ferroelectric hysteresis loops because of the electro-magnetic coupling induced by the MF/FE structure. The remnant magnetization (2Mr) and remnant polarization (2Pr) were 4.6 emu/cm³ and 62 μC/cm², respectively...
November 20, 2017: Materials
https://www.readbyqxmd.com/read/29156594/chemical-transformations-in-proto-cytoplasmic-media-phosphorus-coupling-in-the-silica-hydrogel-phase
#3
Ian B Gorrell, Timothy W Henderson, Kamal Albdeery, Philip M Savage, Terence P Kee
It has been proposed that prebiotic chemical studies on the emergence of primitive life would be most relevant when performed in a hydrogel, rather than an aqueous, environment. In this paper we describe the ambient temperature coupling of phosphorus oxyacids [Pi] mediated by Fe(II) under aerobic conditions within a silica hydrogel (SHG) environment. We have chosen to examine SHGs as they have considerable geological precedence as key phases in silicification en route to rock formation. Following a description of the preparation and characterization studies on our SHG formulations, coupling experiments between Pi species are described across multiple permutations of (i) Pi compound; (ii) gel formulation; (iii) metal salt additive; and (iv) pH-modifying agent...
November 19, 2017: Life
https://www.readbyqxmd.com/read/29156237/skeleton-skin-structured-rgo-cnts-pani-composite-fiber-electrodes-with-excellent-mechanical-and-electrochemical-performance-for-all-solid-state-symmetric-supercapacitors
#4
Dong Liu, Pengcheng Du, Wenli Wei, Hongxing Wang, Qi Wang, Peng Liu
Polyaniline coated reduced graphene oxide/carbon nanotube composite fibers ((RGO/CNTs)@PANI, RCP) with skeleton/skin structure are designed as fiber-shaped electrodes for high performance all-solid-state symmetric supercapacitor. The one-dimensional reduced graphene oxide/carbon nanotube composite fibers (RGO/CNTs, RC) are prepared via a simple in-situ reduction of graphene oxide in presence of carbon nanotubes in quartz glass pipes, which exhibit excellent mechanical performance of >193.4 MPa of tensile strength...
November 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29155749/immobilization-of-caenorhabditis-elegans-to-analyze-intracellular-transport-in-neurons
#5
Shinsuke Niwa
Axonal transport and intraflagellar transport (IFT) are essential for axon and cilia morphogenesis and function. Kinesin superfamily proteins and dynein are molecular motors that regulate anterograde and retrograde transport, respectively. These motors use microtubule networks as rails. Caenorhabditis elegans (C. elegans) is a powerful model organism to study axonal transport and IFT in vivo. Here, I describe a protocol to observe axonal transport and IFT in living C. elegans. Transported cargo can be visualized by tagging cargo proteins using fluorescent proteins such as green fluorescent protein (GFP)...
October 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155734/structural-characterization-of-mannan-cell-wall-polysaccharides-in-plants-using-pace
#6
Venkataramana R Pidatala, Amir Mahboubi, Jenny C Mortimer
Plant cell wall polysaccharides are notoriously difficult to analyze, and most methods require expensive equipment, skilled operators, and large amounts of purified material. Here, we describe a simple method for gaining detailed polysaccharide structural information, including resolution of structural isomers. For polysaccharide analysis by gel electrophoresis (PACE), plant cell wall material is hydrolyzed with glycosyl hydrolases specific to the polysaccharide of interest (e.g., mannanases for mannan). Large format polyacrylamide gels are then used to separate the released oligosaccharides, which have been fluorescently labeled...
October 16, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155597/aggregation-and-rheological-behavior-of-the-lavender-oil-pluronic-p123-microemulsions-in-water-ethanol-mixed-solvents
#7
Oyais Ahmad Chat, Nighat Nazir, Parvaiz Ahmad Bhat, Puthusserickal Abdulrahiman Hassan, Vinod Kumar Aswal, Aijaz Ahmad Dar
The effect of lavender oil on aggregation characteristics of P123 in aqueous-ethanolic solutions is investigated systematically by DLS, SANS and Rheology. The solubilization capacity of the formulation increased by increasing P123 concentration. The study unveiled the importance of the short chain alcohol-ethanol, as solubilization enhancer. The apparent hydrodynamic radius (Rh) increased significantly with increase in lavender oil concentration up to maximum oil solubilization capacity of the copolymer at a particular ethanol concentration...
November 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29155332/unusual-features-of-the-large-linear-plasmid-psa3239-from-streptomyces-aureofaciens-ccm-3239
#8
Erik Mingyar, Renata Novakova, Renata Knirschova, Lubomira Feckova, Carmen Bekeova, Jan Kormanec
We previously identified the aur1 gene cluster, responsible for the production of the angucycline antibiotic auricin in Streptomyces aureofaciens CCM 3239. Pulse-field gel electrophoresis showed a single, 241kb linear plasmid, pSA3239, in this strain, and several approaches confirmed the presence of the aur1 cluster in this plasmid. We report here the nucleotide sequence of this 241,076-bp plasmid. pSA3239 contains an unprecedentedly small (13bp) telomeric sequence CCCGCGGAGCGGG, which is identical to the conserved Palindrome I sequence involved in the priming of end-patching replication...
November 16, 2017: Gene
https://www.readbyqxmd.com/read/29155299/evaluation-of-inositol-phosphates-in-urine-after-topical-administration-of-myo-inositol-hexaphosphate-to-female-wistar-rats
#9
F Grases, A Costa-Bauzá, F Berga, A Rodríguez, R M Gomila, G Martorell, M R Martínez-Cignoni
AIMS: Previous studies demonstrated a remarkable increase of urinary InsP6 by topical administration. However, the methodology used for InsP6 analysis was not specific. The aim of this paper is to measure urinary inositol phosphates InsPs using more advanced methodologies and to compare the results with those obtained by the non-specific method. MATERIALS AND METHODS: We fed 12 female rats with a diet without InsP6 for 16days. Then, we administered a topical InsP6 gel at high doses for 7days (50mgInsP6/day) or at low doses for 28days (20mgInsP6/day)...
November 16, 2017: Life Sciences
https://www.readbyqxmd.com/read/29155274/polysaccharides-from-the-south-african-medicinal-plant-artemisia-afra-structure-and-activity-studies
#10
Paula Marie Braünlich, Kari Tvete Inngjerdingen, Marit Inngjerdingen, Quinton Johnson, Berit Smestad Paulsen, Wilfred Mabusela
Artemisia afra (Jacq. Ex. Willd), is an indigenous plant in South Africa and other parts of the African continent, where it is used as traditional medicine mostly for respiratory conditions. The objective of this study was to investigate the structural features of the polysaccharides from the leaves of this plant, as well as the biological activities of the polysaccharide fractions against the complement assay. Leaves of Artemisia afra were extracted sequentially with organic solvents (dichloromethane and methanol), 50% aqueous ethanol, and water at 50 and 100°C respectively...
November 15, 2017: Fitoterapia
https://www.readbyqxmd.com/read/29155263/identification-and-characterisation-of-the-epididymal-proteins-in-the-lizard-eutropis-carinata-reptilia-squamata-schneider-1801
#11
R Medini, M Bhagya, S Samson
Lizards are seasonal breeders. Cyclic reproductive nature makes lizard as a useful model for the study of the reproductively active protein secretions in the epididymis. During breeding season, the epididymides of the lizard secret proteins that mixes with the spermatozoa and create a favourable environment for sperm maturation. In this spectrum, the aim of this study is to identify and characterize proteins which are present in the lumen of the epididymis of the lizard, E. carinata during the active phase of reproduction...
November 16, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29155199/physiochemical-and-functional-properties-of-tiger-puffer-takifugu-rubripes-skin-gelatin-as-affected-by-extraction-conditions
#12
Jinfeng Pan, Qi Li, Hui Jia, Lining Xia, Wengang Jin, Meijun Shang, Chang Xu, Xiuping Dong
The study investigated physiochemical and functional properties of gelatins from tiger puffer skin (GTPS) extracted with warm-water method at 45, 55 and 65°C for 3, 6 and 12h. GTPS45 exhibited strong γ-, β-, α1- and α2-chains, but they faded with the presence of fragments (<80 KDa) in other GTPS. As the increase of temperature and time, amide I and A were shifted to higher wavenumber and their amplitudes in GTPS65-12 decreased. Lightness declined while redness and yellowness were enhanced. Gel strength was in a range of 144...
November 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29155159/purification-characterization-and-anti-tumor-activities-of-polysaccharides-extracted-from-wild-russula-griseocarnosa
#13
Yang Liu, Jinjin Zhang, Zhaoli Meng
The anti-tumor activity of a novel polysaccharide, PRG1-1, obtained from Russula griseocarnosa sporocarp was investigated in this paper. PRG1-1 has a molecular weight of 630kDa and was extracted and purified using DEAE-cellulose and gel filtration chromatography from crude polysaccharide extract of R. griseocarnosa sporocarp. PRG1-1 was composed of glucose, galactose, mannose, xylose and fructose, in a molar ratio of 66.5:29.2:3.17: 0.663:0.447, respectively. Purified PRG1-1 significantly reduced cell viability, increased the production of lactate dehydrogenase (LDH) and reactive oxygen species (ROS), and enhanced the apoptotic rate in HeLa and SiHa cells...
November 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29155105/functional-diversification-of-sea-lamprey-globins-in-evolution-and-development
#14
Angela Fago, Kim Rohlfing, Elin E Petersen, Agnieszka Jendroszek, Thorsten Burmester
Agnathans have a globin repertoire that markedly differs from that of jawed (gnathostome) vertebrates. The sea lamprey (Petromyzon marinus) harbors at least 18 hemoglobin, two myoglobin, two globin X, and one cytoglobin genes. However, agnathan hemoglobins and myoglobins are not orthologous to their cognates in jawed vertebrates. Thus, blood-based O2 transport and muscle-based O2 storage proteins emerged twice in vertebrates from a tissue-globin ancestor. Notably, the sea lamprey displays three switches in hemoglobin expression in its life cycle, analogous to hemoglobin switching in vertebrates...
November 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29154875/optimization-of-pegylation-reaction-time-and-molar-ratio-of-rhg-csf-toward-increasing-bioactive-potency-of-monopegylated-protein
#15
Jamila Behi, RymHassiki, Nadia Ben Said, Balkiss Bouhaouala-Zahar, Mohamed Lamine Benkhoud
PEGylation is one of the strategies used for enhancing in vivo residence time of recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) and therefore reducing in dose frequency to better fit with patient comfort treatment. In this study, three methoxy polyethylene glycol propionaldehydes (mPEG- ALD) of 10, 20 and 30kDa MW were utilized to produce biologically active monoPEGylated rhG-CSF with enhanced molecular weight. PEGylation reactions were carried out at room temperature and pH 5.0 in the presence of cyanoborohydride and two mPEG-ALD: protein molar ratios (3:1 and 5:1)...
November 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29154803/new-scale-down-methodology-from-commercial-to-lab-scale-to-optimize-plant-derived-soft-gel-capsule-formulations-on-a-commercial-scale
#16
Sana Oishi, Shin-Ichiro Kimura, Shuji Noguchi, Mio Kondo, Yosuke Kondo, Yoshiyuki Shimokawa, Yasunori Iwao, Shigeru Itai
A new scale-down methodology from commercial rotary die scale to laboratory scale was developed to optimize a plant-derived soft gel capsule formulation and eventually manufacture superior soft gel capsules on a commercial scale, in order to reduce the time and cost for formulation development. Animal-derived and plant-derived soft gel film sheets were prepared using an applicator on a laboratory scale and their physicochemical properties, such as tensile strength, Young's modulus, and adhesive strength, were evaluated...
November 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29154473/influences-of-quorum-quenching-probiotic-bacteria-on-the-gut-microbial-community-and-immune-function-in-weaning-pigs
#17
Jonggun Kim, Jaepil Kim, Younghoon Kim, Sangnam Oh, Minho Song, Jee Hwan Choe, Kwang-Youn Whang, Kwang Hyun Kim, Sejong Oh
The aim of this study is to investigate the dynamic gut microbial diversity in weaning swine after administering feed supplemented with probiotic bacteria that specifically inhibit the activity of quorum molecules. Initially, the universal quorum molecule autoinducer-2 (AI-2) bioassay results indicated that AI-2 activity was profoundly inhibited in enterohemorrhagic Escherichia coli (EHEC) O157:H7 in the presence of Lactobacillus acidophilus strain 30SC cell extract, although the growth of EHEC was not affected...
November 20, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29154218/proteomics-of-dark-cutting-longissimus-thoracis-muscle-from-heifer-and-steer-carcasses
#18
Shahid Mahmood, Nancy Turchinsky, Francois Paradis, Walter T Dixon, Heather L Bruce
Studies have suggested that the phenomenon of dark cutting (Canada B4) beef may also be related to muscle glycolytic proteins. The objective of this study, therefore, was to analyze longissimus thoracis (LT; n=23), from Canada AA (n=8), atypical (AB4; pH<5.9, n=8) and typical (TB4; pH>5.9, n=7) B4 heifer and steer carcasses, for sarcoplasmic and myofibrillar proteins using 2-D gel electrophoresis and LC-MS/MS mass spectrometry. Results indicated that AB4 LT had intramuscular pH and lactate concentration similar to Canada AA but lower (P<0...
November 10, 2017: Meat Science
https://www.readbyqxmd.com/read/29153956/effect-of-laser-activated-bleaching-on-the-chemical-stability-and-morphology-of-intracoronal-dentin
#19
Fabiane Carneiro Lopes, Renato Roperto, Anna Akkus, Ozan Akkus, Regina Guenka Palma-Dibb, Manoel Damião de Sousa-Neto
OBJECTIVES: To evaluate the effect of the bleaching with 35% hydrogen peroxide either activated or not by a 970nm diode laser on the chemical stability and dentin surface morphology of intracoronary dentin. METHODS: Twenty-seven slabs of intracoronary dentin specimens (3×3mm) were distributed into three groups (n=9), according to surface treatment: HP - 35% hydrogen peroxide (1×4'), DL - 970nm diode laser (1×30"/0,8W/10Hz), HP+DL - 35% HP activated with 970nm diode laser (1×30"/0,8W/10Hz leaving the gel in contact to the surface for 4' after activation)...
November 13, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29153027/annals-express-large-abnormal-peak-on-capillary-zone-electrophoresis-due-to-contrast-agent
#20
Rachel D Wheeler, Liqun Zhang, Joanna Sheldon
BACKGROUND: Some iodinated radio-contrast media absorb UV light and can therefore be detected by capillary zone electrophoresis (CZE). If seen, these peaks are typically small with "quantifications" of below 5g/L. Here we describe detection of a large peak on CZE that was due to the radio-contrast agent, Omnipaque(T)(M). METHODS: Serum from a patient was analysed by CZE, and the IgG, IgA, IgM and total protein concentrations were measured. The serum sample was further analysed by gel electrophoresis and immunofixation...
January 1, 2017: Annals of Clinical Biochemistry
keyword
keyword
120152
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"