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https://www.readbyqxmd.com/read/28637330/occurrence-of-aminoglycoside-modifying-enzymes-ames-among-isolates-of-escherichia-coli-exhibiting-high-levels-of-aminoglycoside-resistance-isolated-from-korean-cattle-farms
#1
Kuastros Mekonnen Belaynehe, Seung Won Shin, Park Hong-Tae, Han Sang Yoo
This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found resistant to various antibiotics by disc-diffusion. Of the 247 strains 139 (56.3%) were resistant to streptomycin and other antibiotics resistance followed as tetracycline (12.15%), ampicillin (7%), chloramphenicol (5.7%) and trimethoprim-sulfamethoxazole (0...
June 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28637225/characteristics-of-escherichia-coli-isolated-from-broiler-chickens-with-colibacillosis-in-commercial-farms-from-a-common-hatchery
#2
H Ozaki, Y Matsuoka, E Nakagawa, T Murase
To investigate the epidemiologic aspects of colibacillosis in broiler chickens, 83 Escherichia coli isolates obtained from the pericarditis and perihepatitis lesions in broiler chickens from 4 commercial farms, 5 isolates recovered from 5 samples of yolk sac contents that were pooled from 25 emaciated chicks, and 4 fecal isolates obtained from a hatchery that supplied chicks to the 4 commercial farms mentioned above were genetically and bacteriologically characterized. Using pulsed-field gel electrophoresis (PFGE), a total of 92 isolates were classified into 33 pulsotypes...
June 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28636894/effects-of-hpmc-substituent-pattern-on-water-up-take-polymer-and-drug-release-an-experimental-and-modelling-study
#3
Diego Caccavo, Gaetano Lamberti, Anna Angela Barba, Susanna Abrahmsén-Alami, Anna Viridén, Anette Larsson
The purpose of this study was to investigate the hydration behavior of two matrix formulations containing the cellulose derivative hydroxypropyl methylcellulose (HPMC). The two HPMC batches investigated had different substitution pattern along the backbone; the first one is referred to as heterogeneous and the second as homogenous. The release of both the drug molecule theophylline and the polymer was determined. Additionally, the water concentrations at different positions in the swollen gel layers were determined by Magnetic Resonance Imaging...
June 18, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28636892/topical-bioavailability-of-diclofenac-from-locally-acting-dermatological-formulations
#4
S F Cordery, A Pensado, W S Chiu, M Z Shehab, A L Bunge, M B Delgado-Charro, R H Guy
Assessment of the bioavailability of topically applied drugs designed to act within or beneath the skin is a challenging objective. A number of different, but potentially complementary, techniques are under evaluation. The objective of this work was to evaluate in vitro skin penetration and stratum corneum tape-stripping in vivo as tools with which to measure topical diclofenac bioavailability from three approved and commercialized products (two gels and one solution). Drug uptake into, and its subsequent clearance from, the stratum corneum of human volunteers was used to estimate the input rate of diclofenac into the viable skin layers...
June 18, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28636666/molecular-and-microbiological-report-of-a-hospital-outbreak-of-ndm-1-carrying-enterobacteriaceae-in-mexico
#5
Paola Bocanegra-Ibarias, Elvira Garza-González, Rayo Morfín-Otero, Humberto Barrios, Licet Villarreal-Treviño, Eduardo Rodríguez-Noriega, Ulises Garza-Ramos, Santiago Petersen-Morfin, Jesus Silva-Sanchez
OBJECTIVES: To characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital in Mexico. METHODS: From September 2014 to July 2015, all CRE clinical isolates recovered during an outbreak in the Hospital Civil "Fray Antonio Alcalde" in Jalisco, Mexico were screened for antimicrobial susceptibility, carbapenemase production, carbapenemase-encoding genes, and plasmid profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28636390/application-of-a-heterogeneous-chiral-titanium-catalyst-derived-from-silica-supported-3-aryl-h8-binol-to-enantioselective-alkylation-and-arylation-of-aldehydes
#6
Junichiro Akai, Satoshi Watanabe, Kumiko Michikawa, Toshiro Harada
A 3-aryl H8-BINOL was grafted on the surface of silica gel using a hydrosilane derivative as a precursor, and the resulting silica-supported ligand (6 mol %) was employed in the enantioselective alkylation and arylation of aldehydes in the presence of Ti(O(i)Pr)4. The reactions using Et2Zn, Et3B, and aryl Grignard reagents all afforded the corresponding adducts in high enantioselectivities and yields. The silica-immobilized titanium catalyst could be reused up to 14 times without appreciable deterioration of the activity...
June 21, 2017: Organic Letters
https://www.readbyqxmd.com/read/28636347/the-structure-and-dynamics-of-alginate-gels-crosslinked-by-polyvalent-ions-probed-via-solid-state-nmr-spectroscopy
#7
Jiří Brus, Martina Urbanová, Jiri Czernek, Miroslava Pavelkova, Katerina Kubova, Jakub Vyslouzil, Sabina Abbrent, Rafal Konefal, Jiri Horsky, David Vetchy, Jan Vysloužil, Pavel Kulich
Alginate gels are an outstanding biomaterial widely applicable in tissue engineering, medicine, and pharmacy for cell transplantation, wound healing and efficient bioactive agent delivery, respectively. This contribution provides new and comprehensive insight into the atomic-resolution structure and dynamics of polyvalent ion-crosslinked alginate gels in microbead formulations. By applying various advanced solid-state NMR (ssNMR) spectroscopy techniques, we verified the homogenous distribution of the crosslinking ions in the alginate gels and the high degree of ion exchange...
June 21, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28635301/stem-cell-mediated-paracrine-signaling-alters-fibroplasia-in-human-vocal-fold-fibroblasts-in-vitro
#8
Nao Hiwatashi, Renjie Bing, Iv Kraja, Ryan C Branski
OBJECTIVES: Interactions between mesenchymal stem cells (MSCs) and native vocal fold fibroblasts (VFFs) have not been described in spite of promising preliminary data regarding the effects of MSCs on vocal fold repair in vivo. The current study employed a conditioned media (CM) model to investigate the paracrine effects of bone marrow-derived mesenchymal stem cells (BMSCs) on VFFs. METHODS: Human VFFs were treated with transforming growth factor-β1 (TGF-β1; 10 ng/mL), CM from human BMSCs following 48 hours of TGF-β1 stimulation, or CM+TGF-β1...
June 1, 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28635255/characterization-and-immunomodulatory-activity-of-a-novel-peptide-ecfsta-from-wheat-germ-globulin
#9
Wenjia Wu, Mengmeng Zhang, Yao Ren, Xinze Cai, Zhina Yin, Xiaoai Zhang, Tian Min, Hui Wu
A novel peptide was extracted from wheat germ globulin and purified using ion-exchange chromatography, gel filtration chromatography and semi-preparative reverse phase-high performance liquid chromatography (RP-HPLC). The sequence of the peptide was found to be Glu-Cys-Phe-Ser-Thr-Ala (ECFSTA). Its immunomodulatory effects were evaluated, and the results showed that ECFSTA could enhance phagocytosis of RAW 264.7 cells and significantly increase their secretion of nitric oxide (NO), interleukins-6 (IL-6), tumor necrosis factors-α (TNF-α), and reactive oxygen species (ROS)...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635153/topical-silymarin-administration-for-prevention-of-capecitabine-induced-hand-foot-syndrome-a-randomized-double-blinded-placebo-controlled-clinical-trial
#10
Sepideh Elyasi, Farzaneh Sadat Rezazadeh Shojaee, Abolghasem Allahyari, Gholamreza Karimi
Hand-foot syndrome (HFS) is a frequent dose-limiting adverse reaction of capecitabine in patient with gastrointestinal cancers. Silymarin is a polyphenolic flavonoid extracted from the Silybum marianum that exhibits strong antioxidant and antiinflammatory activities. In this study, we evaluated silymarin efficacy in prevention of capecitabine-induced HFS in patients with gastrointestinal cancers, as the first human study. During this pilot, randomized, double-blinded, placebo-controlled clinical trial, the effect of silymarin gel 1%, which is applied on the palms and soles twice daily starting at the first day of chemotherapy for 9 weeks, on HFS occurrence was assessed...
June 21, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28634633/erratum-to-nd-yag-laser-irradiation-associated-with-fluoridated-gels-containing-photo-absorbers-in-the-prevention-of-enamel-erosion
#11
L G S Pereira, S H Joao-Souza, S J C Bezerra, A B Borges, A C C Aranha, T Scaramucci
No abstract text is available yet for this article.
June 20, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28634601/an-annealing-free-aqueous-processed-anatase-tio2-compact-layer-for-efficient-planar-heterojunction-perovskite-solar-cells
#12
Chengwu Yang, Mingyu Yu, Dichun Chen, Yaqing Zhou, Wei Wang, Yang Li, Tung-Chun Lee, Daqin Yun
A facile aqueous-based fabrication scheme is developed for producing annealing-free anatase TiO2 (AF-TiO2) films that exhibit efficient electron transport properties in planar heterojunction perovskite solar cells (PSCs). AF-TiO2 films are fabricated by spin coating on a substrate a colloidal solution of anatase TiO2 nanoparticles (NPs) prepared via a low temperature hydrolytic sol-gel method. The resultant AF-TiO2 films show low electrical resistance, high transmittance in the visible and near-infrared regions and facilitation of high-quality perovskite film formation, which can be attributed to their homogeneous surface morphology and nanocrystallinity...
June 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28634429/exploration-of-spinal-cord-aging-related-proteins-using-a-proteomics-approach
#13
Koshiro Kamiya, Takeo Furuya, Masayuki Hashimoto, Chikato Mannoji, Taigo Inada, Mitsutoshi Ota, Satoshi Maki, Yasushi Ijima, Junya Saito, Mitsuhiro Kitamura, Seiji Ohtori, Sumihisa Orita, Kazuhide Inage, Masashi Yamazaki, Masao Koda
How aging affects the spinal cord at a molecular level is unclear. The aim of this study was to explore spinal cord aging-related proteins that may be involved in pathological mechanisms of age-related changes in the spinal cord. Spinal cords of 2-year-old and 8-week-old female Sprague-Dawley rats were dissected from the animals. Protein samples were subjected to 2-dimentional polyacrylamide gel electrophoresis followed by mass spectrometry. Screened proteins were further investigated with immunohistochemistry and Western blotting...
2017: Journal of Experimental Neuroscience
https://www.readbyqxmd.com/read/28634119/an-ultrasensitive-stain-for-negative-protein-detection-in-sds-page-via-4-5-dibromofluorescein
#14
Dongdong Yu, Yang Wang, Shaoqing Zhang, Zhiwei Chen, Mei Xue, Ying Wang, Weitao Cong, Litai Jin, Zhongxin Zhu
A highly sensitive method for brief and economical staining of proteins in 1-D and 2-D sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) by 4',5'-Dibromofluorescein (DBF) was developed in this study. Down to 0.025-0.05ng protein could be detected within 10min (only 2 steps) by DBF stain, which is approximately 10-fold more sensitive than those of Eosin Y (EY) and SYPRO Ruby stains, and 20-fold more sensitive than that of imidazole-zinc (IZ) negative stain. In addition, the LC-MS/MS results indicated that the newly developed staining method is compatible with the downstream protein identification...
June 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28634067/magnetic-graphene-coated-inorganic-organic-hybrid-nanocomposite-for-enhanced-preconcentration-of-selected-pesticides-in-tomato-and-grape
#15
Hamid Rashidi Nodeh, Hassan Sereshti, Hamid Gaikani, Muhammad Afzal Kamboh, Zahra Afsharsaveh
The new magnetic graphene based hybrid silica-N-[3-(trimethoxysilyl)propyl]ethylenediamine (MG@SiO2-TMSPED) nanocomposite was synthesized via sol-gel process, and used as an effective adsorbent in magnetic solid phase extraction (MSPE) of three selected pesticides followed by gas chromatography micro-electron capture detection (GC-μECD). The adsorbent was characterized using Fourier transform-infrared spectroscopy (FT-IR), Brunauer-Emmett-Teller (BET), Barrett-Joyner-Halenda (BJH), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDXS) techniques...
June 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28633871/high-fat-diet-induced-plasma-protein-and-liver-changes-in-obese-rats-can-be-attenuated-by-melatonin-supplementation
#16
Prapimpun Wongchitrat, Paul Klosen, Supitcha Pannengpetch, Kuntida Kitidee, Piyarat Govitrapong, Chartchalerm Isarankura-Na-Ayudhya
Obesity triggers changes in protein expression in various organs that might participate in the pathogenesis of obesity. Melatonin has been reported to prevent or attenuate such pathological protein changes in several chronic diseases. However, such melatonin effects on plasma proteins have not yet been studied in an obesity model. Using a proteomic approach, we investigated the effect of melatonin on plasma protein profiles after rats were fed a high-fat diet (HFD) to induce obesity. We hypothesized that melatonin would attenuate abnormal protein expression in obese rats...
June 2017: Nutrition Research
https://www.readbyqxmd.com/read/28633855/nanocomposites-synthesis-characterization-and-its-application-to-removal-azo-dyes-using-ultrasonic-assisted-method-modeling-and-optimization
#17
Sima Porhemmat, Mehrorang Ghaedi, Ali Reza Rezvani, Mohammad Hossein Ahmadi Azqhandi, Ali Akbar Bazrafshan
S-doped and Cu- and Co-doped TiO2 was synthesized by a sol-gel method and characterized by FE-SEM, XRD, EDX and FTIR. The Co/Cu/S-TiO2 nanocomposite loaded on the activated carbon as new nanoadsorbent was used for simultaneous removal of methylene blue (MB) and sunset yellow (SY) from aqueous solution by ultrasonic-assisted adsorption method. In this work, central composite design (CCD) and adaptive neuro-fuzzy inference system (ANFIS) as a support tool for examining data and making prediction are used to recognize and predict the removal percentage in MB and SY dye solution of different concentrations...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633569/thermal-and-chemical-denaturation-of-colocasia-esculenta-tuber-agglutinin-from-%C3%AE-2%C3%AE-2-to-unfolded-state
#18
Himadri Biswas, Rajagopal Chattopadhyaya
The major tuber storage protein of Colocasia esculenta, is a monocot mannose-binding, widely used dietary lectin, containing two polypeptides of 12.0 and 12.4 kDa. By both gel filtration and dynamic light scattering at pH 7.2, the lectin has a α2β2 form of apparent molecular mass of 48.2 kDa and a hydrodynamic radius of 6.1±0.2 nm; however, at pH 3, it migrates as αβ and has a reduced hydrodynamic radius of 4.6±0.3 nm. Our circular dichroism spectroscopy studies show that the lectin retains approximately 100% of its secondary structure between pH 2-8, going down to ~90% for extreme acidic / alkaline conditions...
June 20, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28633513/gram-scale-production-of-ginsenoside-f1-using-a-recombinant-bacterial-%C3%AE-glucosidase
#19
Dong-Shan An, Chang-Hao Cui, Muhammad Zubair Siddiqi, Hong Shan Yu, Feng-Xie Jin, Song-Gun Kim, Wan-Taek Im
Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare. Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil3082(T). In a systematic, step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1...
June 21, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28633491/optimization-of-inactivated-h5n9-highly-pathogenic-avian-influenza-vaccine-and-inactivated-salmonella-enterica-serovar-typhimurium-vaccine-with-antigen-dose-and-prime-boost-regimen-in-domestic-ducks
#20
Seong-Su Yuk, Eredene-Ochir To, Jung-Hoon Kwon, Jin-Yong Noh, Woo-Tack Hong, Jei-Hyun Jeong, Gyeong-Bin Gwon, Chang-Seon Song
Owing to the increase in the number of diseases affecting ducks and the demand for food safety by consumers, vaccination has become one of the factors that influence duck meat productivity. The highly pathogenic avian influenza (HPAI) virus is one of the most prevalent and causes one of the most lethal diseases in domestic ducks, and Salmonella enterica serovar Typhimurium is a food-borne pathogen persistent in the domestic duck population. To better understand the optimal usage of HPAI and S. enterica serovar Typhimurium vaccines, we aimed to determine antigen dose, oil and gel adjuvant usage with prime-boost regimen, and vaccination age, inducing the best immune response in ducks, without an effect on body weight gain...
June 17, 2017: Poultry Science
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