keyword
MENU ▼
Read by QxMD icon Read
search

Ionized

keyword
https://www.readbyqxmd.com/read/28092855/lc-ms-ms-determination-of-d-mannose-in-human-serum-as-a-potential-cancer-biomarker
#1
Lyndsey White, Jing Ma, Su Liang, Beatriz Sanchez-Espiridion, Dong Liang
Several metabolites in human serum have been identified as potential cancer biomarkers for early detection. This study focuses on the LC-MS/MS method development and validation of d-mannose in human serum. Surrogate blank serum, coupled with stable isotope d-mannose-(13)C6, as internal standard, was used for generating standard curves ranging from 1 to 50μg/mL. Separation was achieved by an Agilent 1200 series HPLC equipped with a SUPELCOGELTM Pb, 6% Crosslinked column with HPLC water as a mobile phase at flow rate of 0...
December 28, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28092839/effect-and-mechanism-of-sorbus-pohuashanensis-hante-hedl-flavonoids-protect-against-arsenic-trioxide-induced-cardiotoxicity
#2
Xiaojin Yu, Zhenyu Wang, Zunpeng Shu, Zhengqing Li, Yuan Ning, Keli Yun, Haina Bai, Ruihai Liu, Wenli Liu
The cardiotoxicity of arsenic trioxide (ATO) limits its clinical application in cancer treatment. Evidences suggest that sorbus has antioxidant activity and its consumption has been linked with improved cardioprotection. In this study, we investigated the cardio-protective effect and mechanisms of Sorbus pohuashanensis (Hante) Hedl. flavonoids (SPF) against ATO in BALB/c mice and H9c2 cells. Eleven major flavonoids were confirmed by ultra-performance liquid chromatography electrospray ionization quadrupole time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS)...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28092630/comparative-monte-carlo-study-on-the-performance-of-integration-and-list-mode-detector-configurations-for-carbon-ion-computed-tomography
#3
Sebastian Meyer, Chiara Gianoli, Lorena Magallanes, Benedikt Kopp, Thomas Tessonnier, Guillaume Landry, George Dedes, Bernd Voss, Katia Parodi
Ion beam therapy offers the possibility of a highly conformal tumor-dose distribution; however, this technique is extremely sensitive to inaccuracies in the treatment procedures. Ambiguities in the conversion of Hounsfield units of the treatment planning x-ray CT to relative stopping power (RSP) can cause uncertainties in the estimated ion range of up to several millimeters. Ion CT (iCT) represents a favorable solution allowing to directly assess the RSP. In this simulation study we investigate the performance of the integration-mode configuration for carbon iCT, in comparison with a single-particle approach under the same set-up...
February 7, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28092104/quantitative-proteomic-analysis-of-gingival-crevicular-fluids-from-deciduous-and-permanent-teeth
#4
Yumi Moriya, Takashi Obama, Toshihiro Aiuchi, Tomomi Sugiyama, Yumiko Endo, Yoko Koide, Emiko Noguchi, Motonori Ishizuka, Mitsuko Inoue, Hiroyuki Itabe, Matsuo Yamamoto
AIM: Gingivitis commonly progresses to periodontitis in permanent dentition but rarely in deciduous teeth. Little is known about the biochemical differences between gingiva of deciduous and permanent teeth. Here we compared the protein profiles of gingival crevicular fluids (GCF) from the gingiva of deciduous and permanent teeth. MATERIALS AND METHODS: Forty children with mixed dentition (Hellman's dental age IIIA) were selected and GCF samples were collected from deciduous cuspids and central incisors in the maxilla...
January 16, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28091797/mass-spectrometric-identification-of-phenol-soluble-modulins-in-the-atcc%C3%A2-43300-standard-strain-of-methicillin-resistant-staphylococcus-aureus-harboring-two-distinct-phenotypes
#5
K S Jang, M Park, J Y Lee, J S Kim
Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300™) is a representative methicillin-resistant S. aureus (MRSA) strain that is used as a quality control strain for testing the methicillin susceptibility of clinical isolates. It has been consistently observed that colonies with two different colors (golden yellow and white) grow from the ATCC stock on blood agar plates. In this study, staphylococcal peptide and protein profiling was performed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry...
January 14, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28091700/computed-tomography-localization-of-the-appendix-in-the-pediatric-population-relative-to-the-lumbar-spine
#6
John Davis, Albert T Roh, Matthew B Petterson, Tammy R Kopelman, Samantha L Matz, Daniel G Gridley, Mary J Connell
BACKGROUND: Computed tomography (CT) is commonly used to evaluate suspected acute appendicitis. Although very effective, CT uses ionizing radiation, exposing patients to an increased risk of cancer. OBJECTIVE: This study assessed the potential for decreasing the field of view of the CT (and therefore the dose to the patient) in the evaluation of suspected acute appendicitis in children. MATERIALS AND METHODS: This study was a retrospective review of prospectively collected data from 212 consecutive patients who underwent CT for suspected acute appendicitis...
January 14, 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28090219/integrated-microfluidic-aptasensor-for-mass-spectrometric-detection-of-vasopressin-in-human-plasma-ultrafiltrate
#7
J Yang, J Zhu, R Pei, J A Oliver, D W Landry, M N Stojanovic, Q Lin
We present a microfluidic aptamer-based biosensor for detection of low-molecular-weight biomarkers in patient samples. Using a microfluidic device that integrates aptamer-based specific analyte extraction, isocratic elution, and detection by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, we demonstrate rapid, sensitive and label-free detection of arginine vasopressin (AVP) in human plasma ultrafiltrate. AVP molecules in complex matrices are specifically captured by an aptamer that is immobilized on microbeads via affinity binding in a microchamber...
July 14, 2016: Analytical Methods: Advancing Methods and Applications
https://www.readbyqxmd.com/read/28089936/biodegradable-lipid-nanoparticles-induce-a-prolonged-rna-interference-mediated-protein-knockdown-and-show-rapid-hepatic-clearance-in-mice-and-nonhuman-primates
#8
Yuta Suzuki, Kenji Hyodo, Takuya Suzuki, Yohei Tanaka, Hiroshi Kikuchi, Hiroshi Ishihara
Lipid nanoparticles based on ionizable lipids have been clinically validated as a means of delivery for RNA interference (RNAi) therapeutics. The ideal properties of RNAi carriers are efficient delivery of oligonucleotides into target cells and rapid elimination after the function is performed. Here, we report that degradable lipid nanoparticles are effective carriers of small interfering RNA (siRNA) and have a high therapeutic index. The newly developed degradable lipid nanoparticles carrying siRNA showed potent gene-silencing activity in mouse hepatocytes (ED50≈0...
January 9, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28089770/structural-pierce-into-molecular-mechanism-underlying-clostridium-perfringens-epsilon-toxin-function
#9
Saeed Khalili, Abolfazl Jahangiri, Zahra Sadat Hashemi, Bahman Khalesi, Meysam Mardsoltani, Jafar Amani
Epsilon toxin of the Clostridium perfringens garnered a lot of attention due to its potential for toxicity in humans, extreme potency for cytotoxicity in mice and lack of any approved therapeutics prescribed for human. However, the intricacies of the Epsilon toxin action mechanism are yet to be understood. In this regard, various in silico tools have been exploited to model and refine the 3D structure of the toxin and its two receptors. The receptor proteins were embedded into designed lipid membranes within an aqueous and ionized environment...
January 12, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28089560/critical-role-of-p2x7-receptors-in-the-neuroinflammation-and-cognitive-dysfunction-after-surgery
#10
Bin Zheng, Renchun Lai, Jun Li, Zhiyi Zuo
Postoperative cognitive dysfunction worsens patient outcome after surgery. Neuroinflammation is a critical neuropathological process for it. We determined the role of P2X7 receptors, proteins that participate in inflammatory response, in the neuroinflammation induction after surgery, and whether the choice of volatile anesthetics affects its occurrence. Eight-week old C57BL/6J or P2X7 receptor knockout male mice were subjected to right carotid arterial exposure under anesthesia with 1.8% isoflurane, 2.5% sevoflurane or 10% desflurane...
January 9, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28089346/detection-of-unusual-very-long-chain-fatty-acid-and-ether-lipid-derivatives-in-the-fibroblasts-and-plasma-of-patients-with-peroxisomal-diseases-using-liquid-chromatography-mass-spectrometry
#11
Shigeo Takashima, Kayoko Toyoshi, Takahiro Itoh, Naomi Kajiwara, Ayako Honda, Akiko Ohba, Shoko Takemoto, Satoshi Yoshida, Nobuyuki Shimozawa
Metabolic changes occur in patients with peroxisomal diseases owing to impairments in the genes involved in peroxisome function. For diagnostic purposes, saturated very-long-chain fatty acids (VLCFAs) such as C24:0 and C26:0, phytanic acid, pristanic acid, and plasmalogens are often measured as metabolic hallmarks. As the direct pathology of peroxisomal disease is yet to be fully elucidated, we sought to explore the fatty acid species that accumulate in patients with peroxisomal diseases. We developed a method for detecting a range of fatty acids implicated in peroxisomal diseases such as Zellweger syndrome (ZS) and X-linked adrenoleukodystrophy (X-ALD)...
January 7, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28089106/-edge-on-moskin-detector-for-stereotactic-beam-measurement-and-verification
#12
Wei Loong Jong, Ngie Min Ung, Ath Vannyat, Zulaikha Jamalludin, Anatoly Rosenfeld, Jeannie Hsiu Ding Wong
Dosimetry in small radiation field is challenging and complicated because of dose volume averaging and beam perturbations in a detector. We evaluated the suitability of the "Edge-on" MOSkin (MOSFET) detector in small radiation field measurement. We also tested the feasibility for dosimetric verification in stereotactic radiosurgery (SRS) and stereotactic radiotherapy (SRT). "Edge-on" MOSkin detector was calibrated and the reproducibility and linearity were determined. Lateral dose profiles and output factors were measured using the "Edge-on" MOSkin detector, ionization chamber, SRS diode and EBT2 film...
January 6, 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28089069/a-sensitive-gas-chromatography-detector-based-on-atmospheric-pressure-chemical-ionization-by-a-dielectric-barrier-discharge
#13
Ansgar T Kirk, Torben Last, Stefan Zimmermann
In this work, we present a novel concept for a gas chromatography detector utilizing an atmospheric pressure chemical ionization which is initialized by a dielectric barrier discharge. In general, such a detector can be simple and low-cost, while achieving extremely good limits of detection. However, it is non-selective apart from the use of chemical dopants. Here, a demonstrator manufactured entirely from fused silica capillaries and printed circuit boards is shown. It has a size of 75×60×25mm(3) and utilizes only 2W of power in total...
December 24, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28088872/prognostic-prediction-by-liver-tissue-proteomic-profiling-in-patients-with-colorectal-liver-metastases
#14
Adalgiza Reyes, Josep Marti, Santiago Marfà, Wladimiro Jiménez, Vedrana Reichenbach, Amalia Pelegrina, Constantino Fondevila, Juan Carlos Garcia Valdecasas, Josep Fuster
AIM: To obtain proteomic profiles in patients with colorectal liver metastases (CRLM) and identify the relationship between profiles and the prognosis of CRLM patients. MATERIALS & METHODS: Prognosis prediction (favorable or unfavorable according to Fong's score) by a classification and regression tree algorithm of surface-enhanced laser desorption/ionization TOF-MS proteomic profiles from cryopreserved CRLM (patients) and normal liver tissue (controls). RESULTS: The protein peak 7371 m/z showed the clearest differences between CRLM and control groups (94...
January 16, 2017: Future Oncology
https://www.readbyqxmd.com/read/28088758/c5-substituents-of-uridines-and-2-thiouridines-present-at-the-wobble-position-of-trna-determine-the-formation-of-their-keto-enol-or-zwitterionic-forms-a-factor-important-for-accuracy-of-reading-of-guanosine-at-the-3-end-of-the-mrna-codons
#15
Elzbieta Sochacka, Elzbieta Lodyga-Chruscinska, Justyna Pawlak, Marek Cypryk, Paulina Bartos, Katarzyna Ebenryter-Olbinska, Grazyna Leszczynska, Barbara Nawrot
Modified nucleosides present in the wobble position of the tRNA anticodons regulate protein translation through tuning the reading of mRNA codons. Among 40 of such nucleosides, there are modified uridines containing either a sulfur atom at the C2 position and/or a substituent at the C5 position of the nucleobase ring. It is already evidenced that tRNAs with 2-thiouridines at the wobble position preferentially read NNA codons, while the reading mode of the NNG codons by R5U/R5S2U-containing anticodons is still elusive...
January 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28088709/characterization-of-smokeless-powders-using-multiplexed-collision-induced-dissociation-mass-spectrometry-and-chemometric-procedures
#16
Kristen L Reese, A Daniel Jones, Ruth Waddell Smith
This work demonstrates a non-targeted mass spectrometry approach for identification of organic compounds in smokeless powders. Unburned powders were removed from various commercial ammunitions of different brand, primer composition, caliber, and age. The unburned powders and corresponding fired residues were analyzed by liquid chromatography-atmospheric pressure chemical ionization-time-of-flight mass spectrometry (LC-APCI-TOFMS). Multiplexed collision-induced dissociation was performed at increasing collision potentials resulting in successive fragmentation that provided structural information for compound identification in a non-targeted manner...
December 21, 2016: Forensic Science International
https://www.readbyqxmd.com/read/28088663/development-and-validation-of-a-sensitive-and-fast-uplc-ms-ms-method-for-simultaneous-determination-of-seven-bioactive-compounds-in-rat-plasma-after-oral-administration-of-guizhi-gancao-decoction
#17
Bin Ji, Limeng Zhuo, Bin Yang, Yang Wang, Lin Li, Miao Yu, Yunli Zhao, Zhiguo Yu
Rapid, sensitive, selective and accurate UPLC-MS/MS method was developed and fully validated for simultaneous determination of cinnamaldehyde, cinnamic acid, 2-methoxy cinnamic acid, glycyrrhizic acid, glycyrrhetinic acid, liquiritigenin and isoliquiritin in rat plasma after oral administration of Guizhi-gancao decoction. Plasma samples were processed with a simple protein precipitation technique using acetonitrile, followed by chromatographic separation using a Thermo Hypersil GOLD C18 column. A 11.0min linear gradient elution was used at a flow rate of 0...
January 9, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28088450/assessment-of-ganglioside-age-related-and-topographic-specificity-in-human-brain-by-orbitrap-mass-spectrometry
#18
Mirela Sarbu, Liana Dehelean, Cristian V A Munteanu, Željka Vukelić, Alina D Zamfir
The gangliosides (GGs) of the central nervous system (CNS) exhibit age and topographic specificity and these patterns may correlate with the functions and pathologies of the brain regions. Here, chloroform extraction, nanoelectrospray (nanoESI) negative ionization, together with Orbitrap high resolution mass spectrometry (MS) determined the topographic and age-related GG specificity in normal adult human brain. Mapping of GG mixtures extracted from 20 to 82 year old frontal and occipital lobes revealed besides a decrease in the GG number with age, a variability of sialylation degree within the brain regions...
January 11, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28088152/theoretical-description-of-circular-dichroism-in-photoelectron-angular-distributions-of-randomly-oriented-chiral-molecules-after-multi-photon-photoionization
#19
R E Goetz, T A Isaev, B Nikoobakht, R Berger, C P Koch
Photoelectron circular dichroism refers to the forward/backward asymmetry in the photoelectron angular distribution with respect to the propagation axis of circularly polarized light. It has recently been demonstrated in femtosecond multi-photon photoionization experiments with randomly oriented camphor and fenchone molecules [C. Lux et al., Angew. Chem., Int. Ed. 51, 4755 (2012) and C. S. Lehmann et al., J. Chem. Phys. 139, 234307 (2013)]. A theoretical framework describing this process as (2+1) resonantly enhanced multi-photon ionization is constructed, which consists of two-photon photoselection from randomly oriented molecules and successive one-photon ionization of the photoselected molecules...
January 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28087976/the-role-of-diode-array-ultraviolet-detection-for-the-identification-of-synthetic-cathinones
#20
Walter F Rowe, Ioan Marginean, Stephanie Carnes, Ira S Lurie
The utility of diode array UV detection for aiding in the identification of synthetic cathinones, including different sub classes and positional isomers is presented. For 35 synthetic cathinones' unique UV spectra are obtained for seven sub classes including mostly beta ketones, where position and type of substitution on benzene rings give rise to differences in UV maxima and relative intensity of the spectral bands. This aspect is key to distinguishing positional isomers which contain differences in R substitution (mono and di) around the benzene ring, which provides complementary information to electron ionization mass spectrometry, where the latter technique cannot distinguish between these types of positional isomers...
January 14, 2017: Drug Testing and Analysis
keyword
keyword
120151
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"