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https://www.readbyqxmd.com/read/28535609/synthesis-of-%C3%AE-galactooligosaccharide-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#1
So Young Oh, So Youn Youn, Myung Soo Park, Hyoung Geun Kim, Nam In Baek, Zhi Peng Li, Geun Eog Ji
Galactooligosaccharides (GOS) are known to be selectively utilized by Bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. In this study, β-GOS are synthesized using bifidobacterial β-galactosidase(G1) purified from recombinant E. coli with a high GOS yield and with high productivity and enhanced bifidogenic activity. The purified recombinant G1 showed maximum production of β-GOS at pH 8.5 and 45°C. A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of the major peaks of the produced β-GOS showed MW of 527 and 689, indicating the synthesis of β-GOS at degrees of polymerization (DP) of 3 and DP4, respectively...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535597/variation-and-accumulation-patterns-of-poly-and-perfluoroalkyl-substances-pfas-in-european-perch-perca-fluviatilis-across-a-gradient-of-pristine-swedish-lakes
#2
Staffan Åkerblom, Nesrin Negm, Pianpian Wu, Kevin Bishop, Lutz Ahrens
This study assessed variations in the concentrations of poly- and perfluoroalkyl substances (PFAS) in European perch (Perca fluviatilis) in Swedish lakes and the extent to which fish size, age and indicators of fish trophic ecology (δ(15)N and δ(13)C) correlate with the sum of individual PFAS concentrations (ΣPFAS). Fish muscle tissue samples (n=80) were taken from six lakes across Sweden and analysed using solid-liquid extraction followed by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535590/emission-of-volatile-organic-compounds-and-production-of-secondary-organic-aerosol-from-stir-frying-spices
#3
Tengyu Liu, Qianyun Liu, Zijun Li, Lei Huo, ManNin Chan, Xue Li, Zhen Zhou, Chak K Chan
Cooking is an important source of volatile organic compounds (VOCs) and a potential source of secondary organic aerosol (SOA) both indoors and outdoors. In this study, VOC emissions from heating corn oil and stir-frying spices (i.e. garlic, ginger, myrcia and zanthoxylum piperitum (Sichuan pepper)) were characterized using an on-line membrane inlet vacuum ultraviolet single-photon ionization time-of-flight mass spectrometer (VUV-SPI-TOFMS). VOC emissions from heating corn oil were dominated by aldehydes, which were enhanced by factors of one order of magnitude when stir-frying spices...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535452/highly-sensitive-uhplc-ms-ms-method-for-the-simultaneous-estimation-of-propafenone-and-its-metabolites-5-hydroxypropafenone-and-n-depropylpropafenone-on-human-dried-blood-spots-technique-and-application-to-a-pharmacokinetic-study
#4
Adinarayana Andy, Raja Reddy Kallem, Ramesh Mullangi, Divya Andy, J V L N Seshagiri Rao
A highly sensitive, rapid and selective UHPLC-MS/MS method has been developed and validated for quantification of the propafenone (PF), 5-hydroxypropafenone (5-OHPF) and N-depropylpropafenone (N-DPF) on human dried blood spot (DBS). The assay procedure involves a solid-liquid extraction of PF, 5-OHPF and N-DPF and amlodipine (internal standard, I.S.) from dried human DBS cards using water and acetonitrile. The chromatographic resolution was achieved on a BEH C18 column using a gradient mobile phase consisting of 0...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28535423/chromatographic-separation-and-detection-methods-of-aloe-arborescens-miller-constituents-a-systematic-review
#5
REVIEW
Jilan A Nazeam, Haidy A Gad, Hala M El-Hefnawy, Abdel-Naser B Singab
Aloe arborescens Miller (Family Asphodelaceae) is a member of genus Aloe, which is used in traditional medicine to cure various diseases. The extracts of the plant have been reported to possess anticancer, immunomodulator, antidiabetic, anti-inflammatory and antioxidant activities. The phytochemical investigations have revealed diverse chemical constituents, including phenolics [anthraquinones, anthrones, pyrones, chromones and coumarins], polysaccharides [arborans [(1-4) linked glucomannans, polysaccharide (A, B and C): (A: a linear (1-6)-O-α-glucan, B: a branching (1-2)-O-l-arabinose with (1-2)-O-d-galactose linkages and C: (1-4)-O-β-mannan with 18% acetyl group)]], glycoproteins and carboxypeptidase enzyme...
April 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28535128/low-and-high-let-ionizing-radiation-induces-delayed-homologous-recombination-that-persists-for-two-weeks-before-resolving
#6
Christopher P Allen, Hirokazu Hirakawa, Nakako Izumi Nakajima, Sophia Moore, Jingyi Nie, Neelam Sharma, Mayumi Sugiura, Yuko Hoki, Ryoko Araki, Masumi Abe, Ryuichi Okayasu, Akira Fujimori, Jac A Nickoloff
Genome instability is a hallmark of cancer cells and dysregulation or defects in DNA repair pathways cause genome instability and are linked to inherited cancer predisposition syndromes. Ionizing radiation can cause immediate effects such as mutation or cell death, observed within hours or a few days after irradiation. Ionizing radiation also induces delayed effects many cell generations after irradiation. Delayed effects include hypermutation, hyper-homologous recombination, chromosome instability and reduced clonogenic survival (delayed death)...
May 23, 2017: Radiation Research
https://www.readbyqxmd.com/read/28534622/solute-partitioning-in-micelles-combining-molecular-dynamics-simulations-cosmomic-and-experiments
#7
Denitsa Yordanova, Eric Ritter, Thomas Gerlach, Jan-Hendrik Jensen, Irina Smirnova, Sven Jakobtorweihen
The partition equilibria of solutes between micelles and an aqueous phase is a key factor in many applications. Depending on the task, many micelle-solute combinations are possible. Therefore, theoretical methods to predict the partition behavior in micellar systems are needed. Here, two predictive methods are evaluated and compared. First, it is shown how molecular dynamics simulations (MD) with the umbrella sampling method can be used to calculate free energy profiles in micellar systems. The second applied method is an extension of the COSMO-RS theory to anisotropic systems termed COSMOmic...
May 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28534560/re-configurable-multi-mode-contained-electrospray-ionization-for-protein-folding-and-unfolding-on-the-millisecond-time-scale
#8
Colbert F Miller, Dmytro S Kulyk, Jongin W Kim, Abraham K Badu-Tawiah
A new reconfigurable contained-electrospray (ES) ion source is described that can be operated in three unique modes (Types I, II and III) and was applied to control the charge state of proteins for subsequent online characterization by mass spectrometry. Using this device, proteins prepared in 100% water were highly charged after exposure to hydrochloric acid vapor. For myoglobin, the shift to a higher charge state occurred faster than the heme cofactor could escape the unfolding protein. This effect reflected in the detection of highly charged holo-myoglobin intermediates (+26), which suggests the modification process occurred on a millisecond time scale (Type I mode)...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28534553/-ionizing-radiation-received-by-patients-with-osteosarcoma-during-intra-arterial-chemotherapy-treatment
#9
N Aznárez-Sanado, M Aznárez-Sanado, L Sierrasesúmaga, J I Bilbao, J M Martí-Climent, A Patiño-García
BACKGROUND: Osteosarcoma paediatric patients are usually treated with intra-arterial chemotherapy (QTia) which is admi-nistered directly to the tumour. This procedure exposes patients to ionizing radiation. Paediatric patients are especially sensitive to this exposure. METHODS: The total amount of ionizing radiation received from QTia administration was quantified in a group of 16 osteo-sarcoma paediatric patients from the Clínica Universidad de Navarra. RESULTS: The median of the total radiation received per patient was 33...
April 30, 2017: Anales del Sistema Sanitario de Navarra
https://www.readbyqxmd.com/read/28534509/a-novel-interaction-of-pak4-with-ppar%C3%AE-to-regulate-nox1-and-radiation-induced-epithelial-to-mesenchymal-transition-in-glioma
#10
D Kesanakurti, D Maddirela, Y K Banasavadi-Siddegowda, T-H Lai, Z Qamri, N K Jacob, D Sampath, S Mohanam, B Kaur, V K Puduvalli
Tumor recurrence in glioblastoma (GBM) is, in part, attributed to increased epithelial-to-mesenchymal transition (EMT) and enhanced tumor cell dissemination in adjacent brain parenchyma after ionizing radiation (IR). EMT is associated with aggressive behavior, increased stem-like characteristics and treatment resistance in malignancies; however, the underlying signaling mechanisms that regulate EMT are poorly understood. We identified grade-dependent p21-activated kinases 4 (PAK4) upregulation in gliomas and further determined its role in mesenchymal transition and radioresistance...
May 22, 2017: Oncogene
https://www.readbyqxmd.com/read/28534157/infrared-laser-ablation-with-vacuum-capture-for-fingermark-sampling
#11
Fabrizio Donnarumma, Eden E Camp, Fan Cao, Kermit K Murray
Infrared laser ablation coupled to vacuum capture was employed to collect material from fingermarks deposited on surfaces of different porosity and roughness. Laser ablation at 3 μm was performed in reflection mode with subsequent capture of the ejecta with a filter connected to vacuum. Ablation and capture of standards from fingermarks was demonstrated on glass, plastic, aluminum, and cardboard surfaces. Using matrix assisted laser desorption ionization (MALDI), it was possible to detect caffeine after spiking with amounts as low as 1 ng...
May 22, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28534063/-the-new-law-on-radiation-protection-as-a-consequence-of-the-eu-safety-standard-of-2013
#12
REVIEW
G Layer
The transformation of a European guideline (2013/59/Euratom) from 2013 into national law requires adaptation of the national statutory regulations. This year, all areas of protection from ionizing radiation will be subject to the new radiation protection law (StrlSchG). Through this, the German X‑ray and Radiation Protection Acts will be combined to form a higher level of authority. The main parts of the StrlSchG will receive a new classification and will be organized according to the exposure scenario: radiation protection in planned exposure scenarios, radiation protection in emergency exposure scenarios, radiation protection in existing exposure scenarios, and the regulation of overall exposure scenarios...
May 22, 2017: Der Radiologe
https://www.readbyqxmd.com/read/28533144/discovery-of-two-p-superfamily-conotoxins-lt9a-and-lt9b-with-different-modifications-on-voltage-sensitive-sodium-channels
#13
Lei Wang, Junliang Liu, Zhenghua Ren, Yu Chen, Anlong Xu
In this work, two P-superfamily conotoxins, lt9a and lt9b, were purified and characterized from the crude venom of Conus litteratus. The amino acid sequences of lt9a and lt9b were determined by the Edman degradation method. It has been suggested that both lt9a and lt9b are produced from the precursor encoded by the gene Lt9.1. During the conotoxin maturation process, different post-translational modifications occurred between lt9a and lt9b. Conotoxin lt9b was predicted to have two prolines that underwent hydroxylation and one glutamate that underwent carboxylation, while lt9a had no hydroxyproline and carboxyglutamate residue...
May 19, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28533029/determination-of-mineral-oil-aromatic-hydrocarbons-moah-in-edible-oils-and-fats-by-online-liquid-chromatography-gas-chromatography-flame-ionization-detection-lc-gc-fid-evaluation-of-automated-removal-strategies-for-biogenic-olefins
#14
Marco Nestola, Torsten C Schmidt
The determination of mineral oil aromatic hydrocarbons (MOAH) in foodstuffs gained in importance over the last years as carcinogenicity cannot be excluded for certain MOAH. The existence of olefins in foodstuffs, such as edible oils and fats, can be problematic for the determination of MOAH by LC-GC-FID. Removal of these interfering substances by HPLC based on polarity differences is not possible. During gas chromatographic separation heavily overloaded peaks are observed rendering the detection of small mineral oil contaminations almost impossible...
May 16, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28532975/emission-of-extensively-drug-resistant-acinetobacter-baumannii-from-hospital-settings-to-the-natural-environment
#15
M Seruga Music, J Hrenovic, I Goic-Barisic, B Hunjak, D Skoric, T Ivankovic
BACKGROUND: Acinetobacter baumannii is a leading emerging pathogen that is frequently recovered from patients during hospital outbreaks. The role of environmental A. baumannii reservoirs is therefore of great concern worldwide. AIM: To investigate the connection between A. baumannii causing hospital outbreaks and environmental isolates from hospital wastewater, urban sewage and river water as the final natural recipient of wastewaters. METHODS: Clinical isolates from patients with hospital-acquired pneumonia and environmental isolates from water were collected during a two-month monitoring period...
April 11, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28532810/species-level-identification-of-coagulase-negative-staphylococcus-spp-from-buffalo-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-cydb-real-time-quantitative-pcr
#16
Lucas J L Pizauro, Camila C de Almeida, Glenn A Soltes, Durda Slavic, Oswaldo D Rossi-Junior, Fernando A de Ávila, Luiz F Zafalon, Janet I MacInnes
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532617/first-french-experiences-of-total-body-irradiations-using-helical-tomotherapy-%C3%A2
#17
R Sun, X Cuenca, R Itti, S Nguyen Quoc, J-P Vernant, J-J Mazeron, C Jenny, M Chea
PURPOSE: Dynamic conformal radiotherapy with helical TomoTherapy(®) (HT) offers a more quantitative paradigm for total body irradiation. Treatment planning, delivery, dose verification of the first French experiences of total body irradiation using helical TomoTherapy(®) are presented. MATERIALS AND METHODS: Patients planned for total body irradiation at our institution from February 2012 to May 2013 were reported. Total body irradiation consisted in a single fraction of 2Gy...
May 19, 2017: Cancer Radiothérapie: Journal de la Société Française de Radiothérapie Oncologique
https://www.readbyqxmd.com/read/28532539/-detection-of-serum-peptides-in-patients-with-lung-squamous-cell-carcinoma-by-maldi-tof-ms-and-analysis-of-their-correlation-with-chemotherapy-efficacy
#18
Guanhua Zhao, Bin Xu, Xiaoyan Li, Chuanhao Tang, Haifeng Qin, Hong Wang, Shaoxing Yang, Weixia Wang, Hongjun Gao, Kun He, Xiaoqing Liu
BACKGROUND: Treatment options for patients with squamous cell carcinoma of the lung (SCC) are limited in chemotherapy. However, not all patients could benefit form standard platinum regimen. Considering the dismal prognosis of patients with advanced SCC, a greater focus on selecting sensitive chemotherapy regimens remains of upmost importance to improve outcomes in this disease. In this study, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to detect pre-chemotherapy serum peptides in advanced lung squamous cell carcinoma patients accepting paclitaxel combined with platinum chemotherapy and to analyze the correlation between serum peptides and chemotherapy efficacy...
May 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28532242/antioxidant-supplementation-a-linchpin-in-radiation-induced-enteritis
#19
Mumtaz Anwar, Shabeer Ahmad, Reyhan Akhtar, Akhtar Mahmood, Safrun Mahmood
Radiation enteritis is one of the most feared complications of abdominal and pelvic regions. Thus, radiation to abdominal or pelvic malignancies unavoidably injures the intestine. Because of rapid cell turnover, the intestine is highly sensitive to radiation injury, which is the limiting factor in the permissible dosage of irradiation. Bowel injuries such as fistulas, strictures, and chronic malabsorption are potentially life-threatening complications and have an impact on patient quality of life. The incidence of radiation enteritis is increasing because of the current trend of combined chemotherapy and radiation...
January 1, 2017: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/28532210/the-linear-nonthreshold-lnt-model-as-used-in-radiation-protection-an-ncrp-update
#20
John D Boice
Purpose/Objective(s): The linear non-threshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated! It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ("Evaluation of the Linear-Nonthreshold Dose-Response Model for Ionizing Radiation"), UNSCEAR 2000, ICRP Publication 99 (2004) and the National Academies BEIR VII Report (NRC 2006). NCRP Scientific Committee 1-25 is reviewing recent epidemiologic studies focusing on dose-response models, including threshold, and the relevance to radiation protection...
May 23, 2017: International Journal of Radiation Biology
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