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https://www.readbyqxmd.com/read/28210651/analysis-of-platelet-rich-plasma-extraction-variations-in-platelet-and-blood-components-between-4-common-commercial-kits
#1
Jane Fitzpatrick, Max K Bulsara, Paul Robert McCrory, Martin D Richardson, Ming Hao Zheng
BACKGROUND: Platelet-rich plasma (PRP) has been extensively used as a treatment in tissue healing in tendinopathy, muscle injury, and osteoarthritis. However, there is variation in methods of extraction, and this produces different types of PRP. PURPOSE: To determine the composition of PRP obtained from 4 commercial separation kits, which would allow assessment of current classification systems used in cross-study comparisons. STUDY DESIGN: Controlled laboratory study...
January 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28202758/endogenous-brain-lipids-inhibit-prion-amyloid-formation-in-vitro
#2
Clare E Hoover, Kristen A Davenport, Davin M Henderson, Mark D Zabel, Edward A Hoover
The normal cellular prion protein (PrP(C)) resides in detergent-resistant outer membrane lipid rafts in which conversion to the pathogenic misfolded form is believed to occur. Once misfolding occurs, the pathogenic isoform polymerizes into highly stable amyloid fibrils. In vitro assays have demonstrated an intimate association between prion conversion and lipids, specifically phosphatidylethanolamine, which is a critical cofactor in the formation of synthetic infectious prions. In the current work, we demonstrate an alternative inhibitory function of lipids in the prion conversion process as assessed in vitro by real-time, quaking induced conversion (RT-QuIC)...
February 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28202394/platelet-rich-plasma-prp-induces-chondroprotection-via-increasing-autophagy-anti-inflammatory-markers-and-decreasing-apoptosis-in-human-osteoarthritic-cartilage
#3
Mayssam Moussa, Daniel Lajeunesse, George Hilal, Oula El Atat, Gaby Haykal, Rim Serhal, Antonio Chalhoub, Charbel Khalil, Nada Alaaeddine
OBJECTIVES: Autophagy constitutes a defense mechanism to overcome aging and apoptosis in osteoarthritic cartilage. Several cytokines and transcription factors are linked to autophagy and play an important role in the degradative cascade in osteoarthritis (OA). Cell therapy such as platelet rich plasma (PRP) has recently emerged as a promising therapeutic tool for many diseases including OA. However, its mechanism of action on improving cartilage repair remains to be determined. The purpose of this study is to investigate the effect of PRP on osteoarthritic chondrocytes and to elucidate the mechanism by which PRP contributes to cartilage regeneration...
February 13, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28193770/%C3%AE-amyloid-prions-and-the-pathobiology-of-alzheimer-s-disease
#4
Joel C Watts, Stanley B Prusiner
Alzheimer's disease (AD) is the most common neurodegenerative disease in humans and will pose a considerable challenge to healthcare systems in the coming years. Aggregation of the β-amyloid (Aβ) peptide within the brain is thought to be an initiating event in AD pathogenesis. Many recent studies in transgenic mice have provided evidence that Aβ aggregates become self-propagating during disease, leading to a cascade of protein aggregation in the brain, which may underlie the progressive nature of AD. The ability to self-propagate and the existence of distinct "strains" reveals that Aβ aggregates exhibit many properties indistinguishable from those of prions composed of PrP(Sc) proteins...
February 13, 2017: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/28147229/identification-and-structural-characterization-of-the-precursor-conformation-of-the-prion-protein-which-directly-initiates-misfolding-and-oligomerization
#5
Roumita Moulick, Jayant B Udgaonkar
To identify and structurally characterize the precursor conformation of the prion protein (PrP), from which misfolding and aggregation directly commence, has been a long-standing goal. Misfolding converts the α-helical, non-pathogenic functional form of PrP to pathogenic, β-structured oligomeric and amyloidogenic forms, which are the cause of prion diseases. Susceptibility to sporadic prion disease correlates well with the propensity of PrP to misfold to cytotoxic, proteinase K resistant oligomeric conformations at low pH...
January 29, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28145687/phototransformation-of-the-herbicide-propanil-in-paddy-field-water
#6
Luca Carena, Marco Minella, Francesco Barsotti, Marcello Brigante, Marco Milan, Aldo Ferrero, Silvia Berto, Claudio Minero, Davide Vione
When irradiated in paddy-field water, propanil (PRP) undergoes photodegradation by direct photolysis, by reactions with ·OH and CO3·-, and possibly also with the triplet states of chromophoric dissolved organic matter. Irradiation also inhibits the non-photochemical (probably biological) degradation of PRP. The dark and light-induced pathways can be easily distinguished because 3,4-dichloroaniline (34DCA, a transformation intermediate of considerable environmental concern) is produced with almost 100% yield in the dark but not at all through photochemical pathways...
February 1, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28145076/low-agreement-between-fresh-and-frozen-thawed-platelet-rich-plasma-in-the-calibrated-automated-thrombogram-assay
#7
M Ljungkvist, S Lövdahl, E Zetterberg, E Berntorp
INTRODUCTION: Thrombin generation tests (TGTs) are considered to give more detailed information of the overall coagulation capability of a patient than clotting-based routine assays. The TGT thrombin generation assay-calibrated automated thrombogram (TGA-CAT) uses both platelet-poor plasma (PPP) and platelet-rich plasma (PRP). Assessing PRP gives more physiological test conditions and is of great interest considering the important role platelets play in haemostasis. However, PRP needs to be assessed close after blood draw/preparation as freezing fragments the platelets...
January 31, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28144628/de-novo-generation-of-a-unique-cervid-prion-strain-using-protein-misfolding-cyclic-amplification
#8
Crystal Meyerett-Reid, A Christy Wyckoff, Terry Spraker, Bruce Pulford, Heather Bender, Mark D Zabel
Substantial evidence supports the hypothesis that prions are misfolded, infectious, insoluble, and protease-resistant proteins (PrP(RES)) devoid of instructional nucleic acid that cause transmissible spongiform encephalopathies (TSEs). Protein misfolding cyclic amplification (PMCA) has provided additional evidence that PrPRes acts as a template that can convert the normal cellular prion protein (PrP(C)) present in uninfected normal brain homogenate (NBH) into the infectious misfolded PrP(RES) isoform. Human PrP(C) has been shown to spontaneously convert to a misfolded pathological state causing sporadic Creutzfeldt-Jakob disease (sCJD)...
January 2017: MSphere
https://www.readbyqxmd.com/read/28130042/intravitreal-bevacizumab-for-the-treatment-of-vitreous-hemorrhage-due-to-proliferative-diabetic-retinopathy
#9
Rupin N Parikh, Anastasia Traband, Anton M Kolomeyer, Brian L VanderBeek, Benjamin J Kim, Albert M Maguire, Alexander J Brucker
PURPOSE: To evaluate the outcomes of intravitreal bevacizumab (IVB) use in patients with a vitreous hemorrhage (VH) secondary to proliferative diabetic retinopathy (PDR). DESIGN: Retrospective, interventional case series. METHODS: Patients who presented to Scheie Eye Institute between January 2008 and January 2015 with a new VH secondary to PDR and treated with IVB were included. Exclusion criteria consisted of IVB treatment prior to the study, a history of pars plana vitrectomy (PPV), and less than one year of follow-up...
January 24, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28119114/evaluation-of-platelet-rich-plasma-gel-potential-in-acceleration-of-wound-healing-duration-in-patients-underwent-pilonidal-sinus-surgery-a-randomized-controlled-parallel-clinical-trial
#10
Saeed Mohammadi, Shirzad Nasiri, Mohammad Hossein Mohammadi, Ashraf Malek Mohammadi, Mohsen Nikbakht, Mahdi Zahed Panah, Hiva Safar, Shayan Mostafaei, Amir Hossein Norooznezhad, Ahmad Reza Soroosh, Kamran Alimoghaddam, Ardeshir Ghavamzadeh
OBJECTIVES: One of the most important surgical issues applied in the treatment of pilonidal sinus disease is wound healing. The aim of this study was to investigate the possible effect of platelet-rich plasma (PRP) gel on accelerating wound healing in these patients. METHODS: In this randomized, controlled, parallel group clinical trial, 110 patients were randomly allocated into two parallel groups with the same size (controls and treatment arm) after meeting inclusion and exclusion criteria...
January 4, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28118747/in-silico-strategies-on-prion-pathogenic-conversion-and-inhibition-from-prp-c-prp-sc
#11
Nataraj S Pagadala, Khajamohiddin Syed, Rakesh Bhat
To date, various therapeutic strategies identified numerous anti-prion compounds and antibodies that stabilize PrP(C), block the conversion of PrP(C)-PrP(Sc) and increased effect on PrP(Sc) clearance. However, no suitable drug has been identified clinically so far due to the poor oral absorption, low blood-brain-barrier [BBB] penetration, and high toxicity. Although some of the drugs were proven to be effective in prion-infected cell culture and whole animal models, none of them increased the rate of survival compared to placebo...
February 2, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28110950/vancomycin-associated-hemorrhagic-occlusive-retinal-vasculitis-clinical-characteristics-of-36-eyes
#12
Andre J Witkin, David F Chang, J Michael Jumper, Steve Charles, Dean Eliott, Richard S Hoffman, Nick Mamalis, Kevin M Miller, Charles C Wykoff
PURPOSE: To expand understanding of presentation, diagnosis, and outcomes of hemorrhagic occlusive retinal vasculitis (HORV). DESIGN: Retrospective case series. PARTICIPANTS: Thirty-six eyes of 23 patients. METHODS: The American Society of Cataract and Refractive Surgery (ASCRS) and the American Society of Retina Specialists (ASRS) formed a joint task force to define clinical characteristics of HORV and to study its prevalence, cause, treatment, and outcomes...
January 19, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28096242/developing-therapeutics-for-prp-prion-diseases
#13
Kurt Giles, Steven H Olson, Stanley B Prusiner
The prototypical PrP prion diseases are invariably fatal, and the search for agents to treat them spans more than 30 years, with limited success. However, in the last few years, the application of high-throughput screening, medicinal chemistry, and pharmacokinetic optimization has led to important advances. The PrP prion inoculation paradigm provides a robust assay for testing therapeutic efficacy, and a dozen compounds have been reported that lead to meaningful extension in survival of prion-infected mice...
January 17, 2017: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/28086781/effects-of-the-intradiscal-implantation-of-stromal-vascular-fraction-plus-platelet-rich-plasma-in-patients-with-degenerative-disc-disease
#14
Comella Kristin, Silbert Robert, Parlo Michelle
BACKGROUND: Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. The SVF contains a mixture of cells including ADSCs and growth factors and has been depleted of the adipocyte (fat cell) population. We evaluated the safety and efficacy of administering SVF and PRP intra-discally into patients with degenerative disc disease. METHODS: A total of 15 patients underwent a local tumescent liposuction procedure to remove approximately 60 ml of fat tissue...
January 13, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28079651/branch-retinal-vein-occlusion-secondary-to-a-retinal-arteriolar-macroaneurysm-a-novel-mechanism-supported-by-multimodal-imaging
#15
Yi Chen, Simon D M Chen, Fred K Chen
BACKGROUND/PURPOSE: To report a case of a branch retinal vein occlusion secondary to a retinal arteriolar macroaneurysm (RAM). METHODS: Retrospective case report describing examination findings, treatment outcome and unique multimodal imaging features demonstrated on fluorescein angiography, optical coherence tomography, optical coherence tomography angiography and adaptive optics photography of the retinal vessels and RAM. RESULTS: A 61-year-old man presented with 20/200 vision in the right eye because of a branch retinal vein occlusion secondary to a RAM...
January 10, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28077650/melanin-or-melanin-like-substance-interacts-with-the-n-terminal-portion-of-prion-protein-and-inhibits-abnormal-prion-protein-formation-in-prion-infected-cells
#16
Taichi Hamanaka, Keiko Nishizawa, Yuji Sakasegawa, Ayumi Oguma, Kenta Teruya, Hiroshi Kurahashi, Hideyuki Hara, Suehiro Sakaguchi, Katsumi Doh-Ura
Prion diseases are progressive fatal neurodegenerative illnesses caused by the accumulation of transmissible abnormal prion protein (PrP). To find treatments for prion diseases, we searched for substances from natural resources that inhibit abnormal PrP formation in prion-infected cells. We found that high-molecular-weight components from insect cuticle extracts reduced abnormal PrP levels. The chemical nature of these components was consistent with that of melanin. In fact, synthetic melanin produced from tyrosine or 3-hydroxy-l-tyrosine inhibited abnormal PrP formation...
January 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28029568/differences-in-levels-of-platelet-derived-microparticles-in-platelet-components-prepared-using-the-platelet-rich-plasma-buffy-coat-and-apheresis-procedures
#17
Egarit Noulsri, Prapaporn Udomwinijsilp, Surada Lerdwana, Viroje Chongkolwatana, Parichart Permpikul
BACKGROUND: There has been an increased interest in platelet-derived microparticles (PMPs) in transfusion medicine. Little is known about PMP status during the preparation of platelet concentrates for transfusion. AIM: The aim of this study is to compare the PMP levels in platelet components prepared using the buffy coat (BC), platelet-rich plasma platelet concentrate (PRP-PC), and apheresis (AP) processes. METHODS: Platelet components were prepared using the PRP-PC and BC processes...
October 25, 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28012636/efficacy-of-platelet-rich-plasma-in-the-treatment-of-knee-osteoarthritis-a-meta-analysis-of-randomized-controlled-trials
#18
Wen-Li Dai, Ai-Guo Zhou, Hua Zhang, Jian Zhang
PURPOSE: To use meta-analysis techniques to evaluate the efficacy and safety of platelet-rich plasma (PRP) injections for the treatment knee of osteoarthritis (OA). METHODS: We performed a systematic literature search in PubMed, Embase, Scopus, and the Cochrane database through April 2016 to identify Level I randomized controlled trials that evaluated the clinical efficacy of PRP versus control treatments for knee OA. The primary outcomes were Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain and function scores...
December 22, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/27998976/sialylation-controls-prion-fate-in-vivo
#19
Saurabh Srivastava, Elizaveta Katorcha, Martin L Daus, Peter Lasch, Michael Beekes, Ilia V Baskakov
Prions or PrP(Sc) are proteinaceous infectious agents that consist of misfolded, self-replicating states of a sialoglycoprotein called the prion protein or PrP(C) The current work tests a new hypothesis that sialylation determines the fate of prions in an organism. To begin, we produced control PrP(Sc) from PrP(C) using protein misfolding cyclic amplification with beads (PMCAb), and also generated PrP(Sc) with reduced sialylation levels using the same method but with partially desialylated PrP(C) as a substrate (dsPMCAb)...
February 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27966263/molecular-and-microarray-based-analysis-of-diversity-among-resting-and-osteogenically-induced-porcine-mesenchymal-stromal-cells-of-several-tissue-origin
#20
Samet Bayraktar, Pascal Jungbluth, René Deenen, Jan Grassmann, Johannes Schneppendahl, Daphne Eschbach, Armin Scholz, Joachim Windolf, Christoph V Suschek, Vera Grotheer
Mesenchymal stromal cells (MSC) play a pivotal role in modern therapeutic approaches in bone healing disorders. Although bone marrow-derived MSC are most frequently used, the knowledge that many other adult tissues represent promising sources for potent MSC has gained acceptance. In the present study, the osteogenic differentiation potential of porcine skin fibroblasts (FB) as well as bone marrow- (BMSC), adipose tissue- (ASC), and dental pulp-derived stromal cells (DSC) were evaluated. However, additional application of BMP-2 significantly elevated the delayed osteogenic differentiation capacity of ASC and FB cultures...
December 14, 2016: Journal of Tissue Engineering and Regenerative Medicine
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