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https://www.readbyqxmd.com/read/29681911/fine-mapping-of-csvyl-conferring-virescent-leaf-through-the-regulation-of-chloroplast-development-in-cucumber
#1
Mengfei Song, Qingzhen Wei, Jing Wang, Wenyuan Fu, Xiaodong Qin, Xiumei Lu, Feng Cheng, Kang Yang, Lu Zhang, Xiaqing Yu, Ji Li, Jinfeng Chen, Qunfeng Lou
Leaf color mutants in higher plants are ideal materials for investigating the structure and function of photosynthetic system. In this study, we identified a cucumber vyl (virescent-yellow leaf) mutant in the mutant library, which exhibited reduced pigment contents and delayed chloroplast development process. F2 and BC1 populations were constructed from the cross between vyl mutant and cucumber inbred line 'Hazerd' to identify that the vyl trait is controlled by a simply recessive gene designated as CsVYL. The CsVYL gene was mapped to a 3...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29681746/breeding-of-lilies-and-tulips-interspecific-hybridization-and-genetic-background
#2
REVIEW
Agnieszka Marasek-Ciolakowska, Tomotaro Nishikawa, Daniel J Shea, Keiichi Okazaki
Lilies and tulips (Liliaceae family) are economically very important ornamental bulbous plants. Here, we summarize major breeding goals, the role of an integrated method of cut-style pollination and fertilization followed by embryo rescue and mitotic and meiotic polyploidization involved in new assortment development. Both crops have been subjected to extensive interspecific hybridization followed by selection. Additionally, spontaneous polyploidization has played a role in their evolution. In lilies, there is a tendency to replace diploids with polyploid cultivars, whereas in tulip a majority of the cultivars that exist today are still diploid except for triploid Darwin hybrid tulips...
January 2018: Breeding Science
https://www.readbyqxmd.com/read/29681745/breeding-of-fragrant-cyclamen-by-interspecific-hybridization-and-ion-beam-irradiation
#3
REVIEW
Hiroshi Ishizaka
Conventional breeding of cyclamen has relied on crossings among Cyclamen persicum cultivars without consideration of the scent of the flowers. Cyclamen purpurascens is a wild species with the most fragrant flowers in the genus Cyclamen. Allodiploid (2 n = 2 x = 41, AB) and allotriploid (2 n = 3 x = 65, AAB) plants have been produced from crosses of diploid and autotetraploid cultivars of C. persicum (2 n = 2 x = 48, AA; 4 x = 96, AAAA) × diploid wild C. purpurascens (2 n = 2 x = 34, BB) by embryo rescue, but are sterile...
January 2018: Breeding Science
https://www.readbyqxmd.com/read/29681080/identification-and-characterisation-of-a-previously-unknown-drought-tolerance-associated-microrna-in-barley
#4
Hui Zhou, Syed Sarfraz Hussain, Michael Hackenberg, Natalia Bazanova, Omid Eini, Jie Li, Perry Gustafson, Bujun Shi
Drought is the most serious abiotic stress, which causes crop losses on worldwide scale. The present study identified a previously unknown microRNA (designated as hvu-miRX) of 21 nucleotides (nt) in barley. Its precursor (designated pre-miRX) and primary transcript (designated pri-miRX) were also identified, with lengths of 73 nt and 559 nt, respectively. The identified upstream sequence of pri-miRX contains both the TATA box and the CAAT box, which are both required for transcription initiation. Transient promoter activation assays showed that the core promoter region of pri-miRX ranged 500 nt from the transcription start site...
April 22, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29681056/a-cytological-approach-to-study-meiotic-recombination-and-chromosome-dynamics-of-arabidopsis-thaliana-male-meiocytes-in-three-dimensions
#5
Aurélie Hurel, Dylan Phillips, Nathalie Vrielynck, Christine Mézard, Mathilde Grelon, Nicolas Christophorou
During meiotic prophase I chromosomes undergo dramatic conformational changes that accompany chromosome condensation, pairing and recombination between homologs. These changes include the anchoring of telomeres to the nuclear envelope and their clustering to form a bouquet. In plants, these events have been studied and illustrated in intact meiocytes of large genome species. Arabidopsis thaliana is an excellent genetic model where major molecular pathways that control synapsis and recombination between homologs have been uncovered...
April 22, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29680862/staygreen-cssgr-is-a-candidate-for-the-anthracnose-colletotrichum-orbiculare-resistance-locus-cla-in-gy14-cucumber
#6
Junsong Pan, Junyi Tan, Yuhui Wang, Xiangyang Zheng, Ken Owens, Dawei Li, Yuhong Li, Yiqun Weng
Map-based cloning identified a candidate gene for resistance to the anthracnose fungal pathogen Colletotrichum orbiculare in cucumber, which reveals a novel function for the highly conserved STAYGREEN family genes for host disease resistance in plants. Colletotrichum orbiculare is a hemibiotrophic fungal pathogen that causes anthracnose disease in cucumber and other cucurbit crops. No host resistance genes against the anthracnose pathogens have been cloned in crop plants. Here, we reported fine mapping and cloning of a resistance gene to the race 1 anthracnose pathogen in cucumber inbred lines Gy14 and WI 2757...
April 21, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29676042/meta-analysis-of-chromosome-scale-crossover-rate-variation-in-eukaryotes-and-its-significance-to-evolutionary-genomics
#7
Quiterie Haenel, Telma G Laurentino, Marius Roesti, Daniel Berner
Understanding the distribution of crossovers along chromosomes is crucial to evolutionary genomics because the crossover rate determines how strongly a genome region is influenced by natural selection on linked sites. Nevertheless, generalities in the chromosome-scale distribution of crossovers have not been investigated formally. We fill this gap by synthesizing joint information on genetic and physical maps across 62 animal, plant, and fungal species. Our quantitative analysis reveals a strong and taxonomically wide-spread reduction of the crossover rate in the center of chromosomes relative to their peripheries...
April 19, 2018: Molecular Ecology
https://www.readbyqxmd.com/read/29675140/the-genome-structure-of-arachis-hypogaea-linnaeus-1753-and-an-induced-arachis-allotetraploid-revealed-by-molecular-cytogenetics
#8
Eliza F de M B do Nascimento, Bruna V Dos Santos, Lara O C Marques, Patricia M Guimarães, Ana C M Brasileiro, Soraya C M Leal-Bertioli, David J Bertioli, Ana C G Araujo
Peanut, Arachis hypogaea (Linnaeus, 1753) is an allotetraploid cultivated plant with two subgenomes derived from the hybridization between two diploid wild species, A. duranensis (Krapovickas & W. C. Gregory, 1994) and A. ipaensis (Krapovickas & W. C. Gregory, 1994), followed by spontaneous chromosomal duplication. To understand genome changes following polyploidy, the chromosomes of A. hypogaea , IpaDur1, an induced allotetraploid ( A. ipaensis × A. duranensis )4x and the diploid progenitor species were cytogenetically compared...
2018: Comparative Cytogenetics
https://www.readbyqxmd.com/read/29675137/variation-in-chromosome-number-and-breeding-systems-implications-for-diversification-in-pachycereus-pringlei-cactaceae
#9
Carina Gutiérrez-Flores, José L León-de la Luz, Francisco J García-De León, J Hugo Cota-Sánchez
Polyploidy, the possession of more than two sets of chromosomes, is a major biological process affecting plant evolution and diversification. In the Cactaceae, genome doubling has also been associated with reproductive isolation, changes in breeding systems, colonization ability, and speciation. Pachycereus pringlei (S. Watson, 1885) Britton & Rose, 1909, is a columnar cactus that has long drawn the attention of ecologists, geneticists, and systematists due to its wide distribution range and remarkable assortment of breeding systems in the Mexican Sonoran Desert and the Baja California Peninsula (BCP)...
2018: Comparative Cytogenetics
https://www.readbyqxmd.com/read/29675009/genomic-structural-variations-affecting-virulence-during-clonal-expansion-of-pseudomonas-syringae-pv-actinidiae-biovar-3-in-europe
#10
Giuseppe Firrao, Emanuela Torelli, Cesare Polano, Patrizia Ferrante, Francesca Ferrini, Marta Martini, Simone Marcelletti, Marco Scortichini, Paolo Ermacora
Pseudomonas syringae pv. actinidiae (Psa) biovar 3 caused pandemic bacterial canker of Actinidia chinensis and Actinidia deliciosa since 2008. In Europe, the disease spread rapidly in the kiwifruit cultivation areas from a single introduction. In this study, we investigated the genomic diversity of Psa biovar 3 strains during the primary clonal expansion in Europe using single molecule real-time (SMRT), Illumina and Sanger sequencing technologies. We recorded evidences of frequent mobilization and loss of transposon Tn6212, large chromosome inversions, and ectopic integration of IS sequences (remarkably ISPsy31, ISPsy36, and ISPsy37)...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29674537/near-complete-genome-sequence-of-pseudomonas-palleroniana-mab3-a-beneficial-1-aminocyclopropane-1-carboxylate-deaminase-producing-bacterium-able-to-promote-the-growth-of-mushrooms-and-plants
#11
Paola Urón, Admir J Giachini, Bernard R Glick, Márcio J Rossi, Francisco X Nascimento
The near-complete genome sequence of Pseudomonas palleroniana MAB3, a 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from an environmental soil Amanita mushroom, is presented here. The genome of P. palleroniana MAB3 contains a single circular chromosome of 6.29 Mb and an average GC content of 60.5%.
April 19, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29673330/modular-assembly-of-transposable-element-arrays-by-microsatellite-targeting-in-the-guayule-and-rice-genomes
#12
José A Valdes Franco, Yi Wang, Naxin Huo, Grisel Ponciano, Howard A Colvin, Colleen M McMahan, Yong Q Gu, William R Belknap
BACKGROUND: Guayule (Parthenium argentatum A. Gray) is a rubber-producing desert shrub native to Mexico and the United States. Guayule represents an alternative to Hevea brasiliensis as a source for commercial natural rubber. The efficient application of modern molecular/genetic tools to guayule improvement requires characterization of its genome. RESULTS: The 1.6 Gb guayule genome was sequenced, assembled and annotated. The final 1.5 Gb assembly, while fragmented (N50  = 22 kb), maps > 95% of the shotgun reads and is essentially complete...
April 19, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29673315/inter-genome-comparison-of-the-quorn-fungus-fusarium-venenatum-and-the-closely-related-plant-infecting-pathogen-fusarium-graminearum
#13
Robert King, Neil Andrew Brown, Martin Urban, Kim E Hammond-Kosack
BACKGROUND: The soil dwelling saprotrophic non-pathogenic fungus Fusarium venenatum, routinely used in the commercial fermentation industry, is phylogenetically closely related to the globally important cereal and non-cereal infecting pathogen F. graminearum. This study aimed to sequence, assemble and annotate the F. venenatum (strain A3/5) genome, and compare this genome with F. graminearum. RESULTS: Using shotgun sequencing, a 38,660,329 bp F. venenatum genome was assembled into four chromosomes, and a 78,618 bp mitochondrial genome...
April 19, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29671916/assembly-and-annotation-of-a-draft-genome-sequence-for-glycine-latifolia-a-perennial-wild-relative-of-soybean
#14
Qiong Liu, Sungyul Chang, Glen L Hartman, Leslie L Domier
Glycine latifolia (Benth.) Newell & Hymowitz (2n=40), one of the 27 wild perennial relatives of soybean, possesses genetic diversity and agronomically favorable traits that are lacking in soybean. Here, we report the 939-Mb draft genome assembly of G. latifolia (PI 559298) using exclusively linked-reads sequenced from a single Chromium library. We organized scaffolds into 20 chromosome-scale pseudomolecules utilizing two genetic maps and the Glycine max (L.) Merr. genome sequence. High copy numbers of putative 91-bp centromere-specific tandem repeats were observed in consecutive blocks within predicted pericentromeric regions on several pseudomolecules...
April 19, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29671328/metabolic-engineering-of-saccharomyces-cerevisiae-for-high-level-production-of-salidroside-from-glucose
#15
Jingjie Jiang, Hua Yin, Shuai Wang, Yibin Zhuang, Shaowei Liu, Tao Liu, Yanhe Ma
Salidroside is an important plant-derived aromatic compound with diverse biological properties. Due to inadequate natural resources, the supply of salidroside is currently limited. In this work, we engineered the production of salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of salidroside in the yeast...
April 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29670641/genome-wide-identification-and-expression-analysis-of-the-cation-diffusion-facilitator-gene-family-in-turnip-under-diverse-metal-ion-stresses
#16
Xiong Li, Yuansheng Wu, Boqun Li, Wenqi He, Yonghong Yang, Yongping Yang
The cation diffusion facilitator (CDF) family is one of the gene families involved in metal ion uptake and transport in plants, but the understanding of the definite roles and mechanisms of most CDF genes remain limited. In the present study, we identified 18 candidate CDF genes from the turnip genome and named them BrrMTP1.1 - BrrMTP12 . Then, we performed a comparative genomic analysis on the phylogenetic relationships, gene structures and chromosome distributions, conserved domains, and motifs of turnip CDFs...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29666780/identification-expression-analysis-and-molecular-modeling-of-iron-deficiency-specific-clone-3-ids3-like-gene-in-hexaploid-wheat
#17
Priyanka Mathpal, Upendra Kumar, Anuj Kumar, Sanjay Kumar, Sachin Malik, Naveen Kumar, H S Dhaliwal, Sundip Kumar
Graminaceous plants secrete hydroxylated phytosiderophores encoded by the genes iron-deficiency-specific clone 2 ( Ids2 ) and iron-deficiency-specific clone 3 ( Ids3 ). An effort was made to identify a putative ortholog of Hodeum vulgare Ids3 gene in hexaploid wheat. The protein structure of TaIDS3 was modeled using homology modeling and structural behavior of modeled structure was analyzed at 20 ns. The simulation trajectory using molecular dynamics simulation suggested the model to be stable with no large fluctuations in residues and local domain level RMSF values (< 2...
April 2018: 3 Biotech
https://www.readbyqxmd.com/read/29664319/accessories-make-the-outfit-accessory-chromosomes-and-other-dispensable-dna-regions-in-plant-pathogenic-fungi
#18
Stefania Bertazzoni, Angela Williams, Darcy A B Jones, Robert A Syme, Kar-Chun Tan, James Kyawzwar Hane
Fungal pathogen genomes can often be divided into core and accessory regions. Accessory regions may be comprised of either accessory regions (ARs) within core chromosomes (CCs), or wholly-dispensable (accessory) chromosomes (ACs). Fungal ACs and ARs typically accumulate mutations and structural rearrangements more rapidly over time than CCs, and many harbour genes relevant to host-pathogen interactions. These regions are of particular interest in plant pathology and include host-specific virulence factors and secondary metabolite synthesis gene clusters...
April 17, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29661190/a-manually-annotated-actinidia-chinensis-var-chinensis-kiwifruit-genome-highlights-the-challenges-associated-with-draft-genomes-and-gene-prediction-in-plants
#19
Sarah M Pilkington, Ross Crowhurst, Elena Hilario, Simona Nardozza, Lena Fraser, Yongyan Peng, Kularajathevan Gunaseelan, Robert Simpson, Jibran Tahir, Simon C Deroles, Kerry Templeton, Zhiwei Luo, Marcus Davy, Canhong Cheng, Mark McNeilage, Davide Scaglione, Yifei Liu, Qiong Zhang, Paul Datson, Nihal De Silva, Susan E Gardiner, Heather Bassett, David Chagné, John McCallum, Helge Dzierzon, Cecilia Deng, Yen-Yi Wang, Lorna Barron, Kelvina Manako, Judith Bowen, Toshi M Foster, Zoe A Erridge, Heather Tiffin, Chethi N Waite, Kevin M Davies, Ella P Grierson, William A Laing, Rebecca Kirk, Xiuyin Chen, Marion Wood, Mirco Montefiori, David A Brummell, Kathy E Schwinn, Andrew Catanach, Christina Fullerton, Dawei Li, Sathiyamoorthy Meiyalaghan, Niels Nieuwenhuizen, Nicola Read, Roneel Prakash, Don Hunter, Huaibi Zhang, Marian McKenzie, Mareike Knäbel, Alastair Harris, Andrew C Allan, Andrew Gleave, Angela Chen, Bart J Janssen, Blue Plunkett, Charles Ampomah-Dwamena, Charlotte Voogd, Davin Leif, Declan Lafferty, Edwige J F Souleyre, Erika Varkonyi-Gasic, Francesco Gambi, Jenny Hanley, Jia-Long Yao, Joey Cheung, Karine M David, Ben Warren, Ken Marsh, Kimberley C Snowden, Kui Lin-Wang, Lara Brian, Marcela Martinez-Sanchez, Mindy Wang, Nadeesha Ileperuma, Nikolai Macnee, Robert Campin, Peter McAtee, Revel S M Drummond, Richard V Espley, Hilary S Ireland, Rongmei Wu, Ross G Atkinson, Sakuntala Karunairetnam, Sean Bulley, Shayhan Chunkath, Zac Hanley, Roy Storey, Amali H Thrimawithana, Susan Thomson, Charles David, Raffaele Testolin, Hongwen Huang, Roger P Hellens, Robert J Schaffer
BACKGROUND: Most published genome sequences are drafts, and most are dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that are not assigned to chromosomes, and predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft genome has 164 Mb of sequences unassigned to pseudo-chromosomes, and omissions have been identified in the gene models. RESULTS: A second genome of an A...
April 16, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29660854/the-butanol-producing-microbe-clostridium-beijerinckii-ncimb-14988-manipulated-using-forward-and-reverse-genetic-tools
#20
Gareth T Little, Benjamin J Willson, John T Heap, Klaus Winzer, Nigel P Minton
The solventogenic anaerobe Clostridium beijerinckii has potential for use in the sustainable bioconversion of plant-derived carbohydrates into solvents, such as butanol or acetone. However, relatively few strains have been extensively characterised either at the genomic level or through exemplification of a complete genetic toolkit. To remedy this situation, a new strain of C. beijerinckii, NCIMB 14988, was selected from amongst a total of 55 new clostridial isolates capable of growth on hexose and pentose sugars...
April 16, 2018: Biotechnology Journal
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