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https://www.readbyqxmd.com/read/29026596/flow-cytometry-of-duodenal-intraepithelial-lymphocytes-improves-diagnosis-of-celiac-disease-in-difficult-cases
#1
Julio Valle, José Mario T Morgado, Juan Ruiz-Martín, Antonio Guardiola, Miriam Lopes-Nogueras, Almudena García-Vela, Beatriz Martín-Sacristán, Laura Sánchez-Muñoz
BACKGROUND: Diagnosis of celiac disease is difficult when the combined results of serology and histology are inconclusive. Studies using flow cytometry of intraepithelial lymphocytes (IELs) have found that celiac patients have increased numbers of γδ IELs, along with a decrease in CD3-CD103 + IELs. OBJECTIVE: The objective of this article is to assess the role of flow cytometric analysis of IELs in the diagnosis of celiac disease in difficult cases. METHODS: A total of 312 patients with suspicion of celiac disease were included in the study...
October 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28965397/a-new-method-for-treating-drude-polarization-in-classical-molecular-simulation
#2
Alex Albaugh, Teresa Head-Gordon
With polarization becoming an increasingly common feature in classical molecular simulation, it is important to develop methods that can efficiently and accurately evaluate the many-body polarization solution. In this work we expand the theoretical framework of our inertial extended Langrangian, self-consistent field iteration-free method (iEL/0-SCF), introduced for point induced dipoles, to the polarization model of a Drude oscillator. When applied to the polarizable simple point charge model (PSPC) for water, our iEL/0-SCF method for Drude polarization is as stable as a well-converged SCF solution and more stable than traditional extended Lagrangian (EL) approaches or EL formulations based on two temperature ensembles where Drude particles are kept "colder" than the real degrees of freedom...
October 2, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28958836/in-vivo-blockade-of-t-cell-development-reveals-alternative-pathways-for-generation-of-intraepithelial-lymphocytes-in-mice
#3
Surenchimeg Mondoon, Kensuke Shibata, Yasunobu Yoshikai
Intraepithelial lymphocytes (IELs) are resident cells localized within the intestinal epithelia and play an important role in regulating gut inflammations and host defense against pathogens. CD8α(+) TCRαβ(+) IELs are heterogeneous populations that are generated from T cell precursors including CD4(-) CD8α(-) double-negative (DN) cells and CD4(+) CD8α(+) double-positive (DP) cells. However, developmental pathways of TCRαβ(+) IELs remained unclear. To gain insight into the mechanisms, we generated mice (Bcl11b(ΔDN2) mice) that lack thymic precursors (DN CD5(+) TCRβ(+) cells) for CD4(-) CD8αα(+) TCRαβ(+) IELs...
November 2017: Immunology Letters
https://www.readbyqxmd.com/read/28951299/hericium-erinaceus-polysaccharide-facilitates-restoration-of-injured-intestinal-mucosal-immunity-in-muscovy-duck-reovirus-infected-muscovy-ducklings
#4
Yijian Wu, Huihui Jiang, Erpeng Zhu, Jian Li, Quanxi Wang, Wuduo Zhou, Tao Qin, Xiaoping Wu, Baocheng Wu, Yifan Huang
To elucidate the effect of Hericium erinaceus polysaccharide (HEP) on the intestinal mucosal immunity in normal and Muscovy duck reovirus (MDRV)-infected Muscovy ducklings, 1-day-old healthy Muscovy ducklings were pretreated with 0.2g/L HEP and/or following by MDRV infection in this study, duodenal samples were respectively collected at 1, 3, 6, 10, 15 and 21day post-infection, tissue sections were prepared for observation of morphological structure and determination of intestinal parameters (villus height/crypt depth ratio, villus surface area) as well as counts of intraepithelial lymphocytes (IELs), goblet cells, mast cells...
September 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28942917/intestinal-epithelial-and-intraepithelial-t-cell-crosstalk-mediates-a-dynamic-response-to-infection
#5
David P Hoytema van Konijnenburg, Bernardo S Reis, Virginia A Pedicord, Julia Farache, Gabriel D Victora, Daniel Mucida
Intestinal intraepithelial lymphocytes (IELs) are located at the critical interface between the intestinal lumen, which is chronically exposed to food and microbes, and the core of the body. Using high-resolution microscopy techniques and intersectional genetic tools, we investigated the nature of IEL responses to luminal microbes. We observed that TCRγδ IELs exhibit unique microbiota-dependent location and movement patterns in the epithelial compartment. This behavioral pattern quickly changes upon exposure to different enteric pathogens, resulting in increased interepithelial cell (EC) scanning, expression of antimicrobial genes, and glycolysis...
September 16, 2017: Cell
https://www.readbyqxmd.com/read/28926545/generating-cd4-cd8%C3%AE-%C3%AE-iels
#6
Laurie A Dempsey
No abstract text is available yet for this article.
September 19, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28922850/comparative-ultrastructural-and-stereological-analyses-of-unruptured-and-ruptured-saccular-intracranial-aneurysms
#7
Emine Korkmaz, Rachel Kleinloog, Bon H Verweij, Iris E Allijn, Liesbeth H P Hekking, Luca Regli, Gabriel J E Rinkel, Ynte M Ruigrok, Jan Andries Post
Insight into processes leading to rupture of intracranial aneurysms (IAs) may identify biomarkers for rupture or lead to management strategies reducing the risk of rupture. We characterized and quantified (ultra)structural differences between unruptured and ruptured aneurysmal walls. Six unruptured and 6 ruptured IA fundi were resected after microsurgical clipping and analyzed by correlative light microscopy for quantitative analysis (proportion of the vessel wall area) and transmission electron microscopy for qualitative ultrastructural analysis...
October 1, 2017: Journal of Neuropathology and Experimental Neurology
https://www.readbyqxmd.com/read/28899494/intranasal-co-administration-of-1-8-cineole-with-influenza-vaccine-provide-cross-protection-against-influenza-virus-infection
#8
Yun Li, Yu-Ling Xu, Yan-Ni Lai, Shang-Hui Liao, Ni Liu, Pei-Ping Xu
BACKGROUND: Vaccination is the most efficient means for protection against influenza. However, the various vaccines have low efficacy to protect against pandemic strains because of antigenic drift and recombination of influenza virus. Adjuvant therapy is one of the attempts to improve influenza vaccine effective cross-protection against influenza virus infection. Our previous study confirmed that 1,8-cineole inhibits the NF-κB, reduces pro-inflammatory cytokines, and relieves the pathological changes of viral pneumonia in mice infected with influenza virus...
October 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28894572/comparison-between-digital-and-optical-microscopy-analysis-in-a-mouse-gut-inflammation-model
#9
Airton Pereira E Silva, Sylvia Maria Nicolau Campos, Isabelle Mazza Guimarães, Gerlinde Agate Platais Brasil Teixeira
Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niterói, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research...
September 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28893865/roc-king-onwards-intraepithelial-lymphocyte-counts-distribution-role-in-coeliac-disease-mucosal-interpretation
#10
Kamran Rostami, Michael N Marsh, Matt W Johnson, Hamid Mohaghegh, Calvin Heal, Geoffrey Holmes, Arzu Ensari, David Aldulaimi, Brigitte Bancel, Gabrio Bassotti, Adrian Bateman, Gabriel Becheanu, Anna Bozzola, Antonio Carroccio, Carlo Catassi, Carolina Ciacci, Alexandra Ciobanu, Mihai Danciu, Mohammad H Derakhshan, Luca Elli, Stefano Ferrero, Michelangelo Fiorentino, Marilena Fiorino, Azita Ganji, Kamran Ghaffarzadehgan, James J Going, Sauid Ishaq, Alessandra Mandolesi, Sherly Mathews, Roxana Maxim, Chris J Mulder, Andra Neefjes-Borst, Marie Robert, Ilaria Russo, Mohammad Rostami-Nejad, Angelo Sidoni, Masoud Sotoudeh, Vincenzo Villanacci, Umberto Volta, Mohammad R Zali, Amitabh Srivastava
OBJECTIVES: Counting intraepithelial lymphocytes (IEL) is central to the histological diagnosis of coeliac disease (CD), but no definitive 'normal' IEL range has ever been published. In this multicentre study, receiver operating characteristic (ROC) curve analysis was used to determine the optimal cut-off between normal and CD (Marsh III lesion) duodenal mucosa, based on IEL counts on >400 mucosal biopsy specimens. DESIGN: The study was designed at the International Meeting on Digestive Pathology, Bucharest 2015...
September 11, 2017: Gut
https://www.readbyqxmd.com/read/28892904/morphologic-spectrum-of-duodenal-biopsies-in-malabsorption-a-study-from-southern-india
#11
Priyavadhana Balasubramanian, Bhawana Ashok Badhe, Rajesh Nachiappa Ganesh, Lakshmi C Panicker, Pazhanivel Mohan
INTRODUCTION: Duodenal endoscopic biopsy is a common investigation for various non-neoplastic conditions. Malabsorption is a common indication for duodenal biopsy in our setting. AIM: Our study was undertaken to study the morphologic spectrum of non-neoplastic conditions of duodenum emphasizing on Intraepithelial Lymphocytes (IELs) and to have a clinico-pathologic correlation. MATERIALS AND METHODS: This was a prospective descriptive study...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28890546/generating-cd8%C3%AE-%C3%AE-iels-from-two-sources-of-thymic-precursors
#12
Lu Bai, Hui Peng
No abstract text is available yet for this article.
September 11, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28878277/increased-tcr-signal-strength-in-dn-thymocytes-promotes-development-of-gut-tcr%C3%AE-%C3%AE-cd8%C3%AE-%C3%AE-intraepithelial-lymphocytes
#13
Capucine L Grandjean, Nital Sumaria, Stefania Martin, Daniel J Pennington
CD4((+))CD8((+)) "double positive" (DP) thymocytes differentiate into diverse αβ T cell sub-types using mechanistically distinct programs. For example, conventional αβ T cells develop from DP cells after partial-agonist T cell receptor (TCR) interactions with self-peptide/MHC, whereas unconventional αβ T cells, such as TCRαβ((+))CD8αα((+)) intraepithelial lymphocytes (IELs), require full-agonist TCR interactions. Despite this, DP cells appear homogeneous, and it remains unclear how distinct TCR signalling instructs distinct developmental outcomes...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28859865/ischemic-stroke-damages-the-intestinal-mucosa-and-induces-alteration-of-the-intestinal-lymphocytes-and-ccl19-mrna-in-rats
#14
Yaning Liu, Shijian Luo, Li Kou, Chaogang Tang, Ruxun Huang, Zhong Pei, Zhendong Li
The immunoreaction has a pivotal effect on ischemic stroke. It has been demonstrated that intestinal lymphocytes infiltrate into the brain and aggravate tissue injury after stroke. However, less attention has been paid to the influence on the intestinal immunology as well as morphology. Here, we utilized a rat permanent middle cerebral artery occlusion (MCAO) model to investigate the influences on intestinal mucosa, lymphocytes of the gut-associated lymphoid tissue (GALT), and the intestinal expression of CCL25 mRNA and CCL19 mRNA of stroke...
September 29, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28852850/antimicrobial-mechanism-of-oleanolic-and-ursolic-acids-on-streptococcus-mutans-ua159
#15
Soon-Nang Park, Yun Kyong Lim, Mi-Hwa Choi, Eugene Cho, Iel Soo Bang, Jung Min Kim, Sug-Joon Ahn, Joong-Ki Kook
Triterpenoid saponin derivatives oleanolic acid (OA) and ursolic acid (UA), but not betulinic acid (BA), were previously found to have strong antimicrobial activity against Streptococcus mutans. OA and UA inhibited the transcription of genes related to peptidoglycan biosynthesis, thereby preventing bacterial growth. However, it is not clear whether this is the only pathway involved in the antimicrobial activity of these compounds against S. mutans. Therefore, we used quantitative real-time PCR (qPCR) and microarray analyses to examine the expression of genes related to essential metabolic pathways in S...
August 29, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28815514/intraepithelial-lymphocyte-immunophenotype-a-useful-tool-in-the-diagnosis-of-celiac-disease
#16
Rebeca Saborido, Nazareth Martinón, Alexandra Regueiro, Vanesa Crujeiras, Pablo Eiras, Rosaura Leis
According to new ESPGHAN guidelines, gluten challenge is considered necessary when there is doubt about the initial diagnosis of celiac disease (CD). The main aim of this study was to quantify intraepithelial lymphocyte (IEL) immunophenotype on celiac patients on gluten-containing diet (GCD) compared to those on gluten-free diet (GFD). Another aim was to evaluate the clinical utility of IELs in the CD diagnosis, especially in selected patients on GFD where diagnostic uncertainty remains. IEL immunophenotype (TCRγδ and NK-like IELs) were studied by flow cytometry in 111 children with CD (81 children with CD on GCD and 30 celiac patients on GFD) and a control group (10 children)...
August 16, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28812548/bacteroidales-recruit-il-6-producing-intraepithelial-lymphocytes-in-the-colon-to-promote-barrier-integrity
#17
K A Kuhn, H M Schulz, E H Regner, E L Severs, J D Hendrickson, G Mehta, A K Whitney, D Ir, N Ohri, C E Robertson, D N Frank, E L Campbell, S P Colgan
Interactions between the microbiota and distal gut are important for the maintenance of a healthy intestinal barrier; dysbiosis of intestinal microbial communities has emerged as a likely contributor to diseases that arise at the level of the mucosa. Intraepithelial lymphocytes (IELs) are positioned within the epithelial barrier, and in the small intestine they function to maintain epithelial homeostasis. We hypothesized that colon IELs promote epithelial barrier function through the expression of cytokines in response to interactions with commensal bacteria...
August 16, 2017: Mucosal Immunology
https://www.readbyqxmd.com/read/28809572/antigen-specific-gut-inflammation-and-systemic-immune-responses-induced-by-prolonging-wheat-gluten-sensitization-in-balb-c-murine-model
#18
M Vijaykrishnaraj, B V Mohan Kumar, S P Muthukumar, Nawneet K Kurrey, P Prabhasankar
Gluten-related diseases such as wheat allergy, celiac disease, and gluten intolerance are widespread around the globe to genetically predisposed individuals. The present study aims to develop a wheat-gluten induced BALB/c murine model for addressing wheat-gluten related disorders by sensitizing the wheat gluten through the route of intraperitoneal and oral challenge in prolonged days. During the sensitization, the sera were collected for specific antigliadin antibodies response and proinflammatory markers quantification...
September 11, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28797734/microrna-150-controls-differentiation-of-intraepithelial-lymphocytes-via-tgf-%C3%AE-receptor-ii-regulation
#19
Sang-Hwan Seo, Min Seong Jang, Doo-Jin Kim, Seok-Min Kim, Se-Chan Oh, Cho-Rok Jung, Yunji Park, Sang-Jun Ha, Haiyoung Jung, Young-Jun Park, Suk Ran Yoon, Inpyo Choi, Tae-Don Kim
BACKGROUND: Intraepithelial lymphocytes (IELs) in the intestines play pivotal roles in maintaining the integrity of the mucosa, regulating the immune cells, and protecting against pathogenic invasion. Although several extrinsic factors such as transforming growth factor-β (TGF-β) have been identified to contribute to IEL generation, intrinsic regulatory factors have not been fully determined. OBJECTIVE: Here, we investigated the regulation of IEL differentiation and the underlying mechanisms in mice...
August 7, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28775213/lactobacillus-reuteri-induces-gut-intraepithelial-cd4-cd8%C3%AE-%C3%AE-t-cells
#20
Luisa Cervantes-Barragan, Jiani N Chai, Ma Diarey Tianero, Blanda DiLuccia, Philip P Ahern, Joseph Merriman, Victor S Cortez, Michael G Caparon, Mohamed S Donia, Susan Gilfillan, Marina Cella, Jeffrey I Gordon, Chyi-Song Hsieh, Marco Colonna
The small intestine contains CD4(+)CD8αα(+) double-positive intraepithelial T lymphocytes (DP IELs), which originate from intestinal CD4(+) T cells through downregulation of the transcription factor ThPOK and have regulatory functions. DP IELs are absent in germ-free mice, suggesting that their differentiation depends on microbial factors. We found that DP IEL numbers in mice varied in different vivaria, correlating with the presence of Lactobacillus reuteri This species induced DP IELs in germ-free mice and conventionally raised mice lacking these cells...
August 3, 2017: Science
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