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isatis indigotica de novo transcriptome

Yingying Zhou, Lei Kang, Shiying Liao, Qi Pan, Xianhong Ge, Zaiyun Li
The giant organs and enhanced concentrations of secondary metabolites realized by autopolyploidy are attractive for breeding the respective medicinal and agricultural plants and studying the genetic mechanisms. The traditional medicinal plant Chinese woad (Isatis indigotica Fort., 2n = 2x = 14) is now still largely used for the diseases caused by bacteria and viruses in China. In this study, its autopolyploids (3x, 4x) were produced and characterized together with the 2x donor for their phenotype and transcriptomic alterations by using high-throughput RNA sequencing...
2015: PloS One
Xiaoqing Tang, Yunhua Xiao, Tingting Lv, Fangquan Wang, QianHao Zhu, Tianqing Zheng, Jie Yang
BACKGROUND: Isatis indigotica, the source of the traditional Chinese medicine Radix isatidis (Ban-Lan-Gen), is an extremely important economical crop in China. To facilitate biological, biochemical and molecular research on the medicinal chemicals in I. indigotica, here we report the first I. indigotica transcriptome generated by RNA sequencing (RNA-seq). RESULTS: RNA-seq library was created using RNA extracted from a mixed sample including leaf and root. A total of 33,238 unigenes were assembled from more than 28 million of high quality short reads...
2014: PloS One
Junfeng Chen, Xin Dong, Qing Li, Xun Zhou, Shouhong Gao, Ruibing Chen, Lianna Sun, Lei Zhang, Wansheng Chen
BACKGROUND: Isatis indigotica is a widely used herb for the clinical treatment of colds, fever, and influenza in Traditional Chinese Medicine (TCM). Various structural classes of compounds have been identified as effective ingredients. However, little is known at genetics level about these active metabolites. In the present study, we performed de novo transcriptome sequencing for the first time to produce a comprehensive dataset of I. indigotica. RESULTS: A database of 36,367 unigenes (average length = 1,115...
December 5, 2013: BMC Genomics
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