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isatis indigotica

Lei Zhang, Junfeng Chen, Xun Zhou, Xiaofei Chen, Qing Li, Hexin Tan, Xin Dong, Ying Xiao, Langdong Chen, Wansheng Chen
A molecular description of lignan biosynthesis in Isatis indigotica displaying its synthetic characteristics and regulatory mechanism is of great importance for the improvement of the production of this class of active compounds. To discover the potential key catalytic steps and regulatory genes, I. indigotica hairy roots elicited by methyl jasmonate (MeJA) were used as a source of systematic variation for exploring the metabolic/ transcriptional changes and candidate genes that might play key roles in lignan biosynthesis...
May 7, 2016: Plant Biotechnology Journal
Hong-ran Dong, Jian Yang, Lu-qi Huang, Jing-ming Jia, Jin-fu Tang
Based on the transcriptome data, we cloned the open reading frame of IiHCT gene from Isatis indigotica, and then performed bioinformatic analysis of the sequence. Further, we detected expression pattern in specific organs and hairy roots treated methyl jasmonate( MeJA) by RT-PCR. The IiHCT gene contains a 1 290 bp open reading frame( ORF) encoding a polypeptide of 430 amino acids. The predicted isoelectric point( pI) was 5.7, a calculated molecular weight was about 47.68 kDa. IiHCT was mainly expressed in stem and undetectable in young root, leaf and flower bud...
November 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Lei Zhang, Qing Li, Jun-Feng Chen, Wan-Sheng Chen
Isatis indigotica Fort., belonging to Cruciferae, is one of the most commonly used plants in traditional Chinese medicine. The accumulation of the effective components of I. indigotica is related with its growth conditions. The GRAS genes are members of a multigene family of transcriptional regulators that play a crucial role in plant growth. Although the activities of many GRAS genes have long been recognized, only in recent years were some of them identified and functionally characterized in detail. In the present study, 41 GRAS genes were identified from I...
March 2016: Chinese Journal of Natural Medicines
Yufeng Liu, Xiaoliang Wang, Minghua Chen, Sheng Lin, Li Li, Jiangong Shi
Three pairs of enantiomerically pure alkaloids with diverse structure features, named isatindigoticoic acid A and epiisatindigoticoic acid A [(-)-1 and (+)-1], phaitanthrin A and epiphaitanthrin A [(-)-2 and (+)-2], and isatindopyrromizol A and epiisatindopyrromizol A [(-)-3 and (+)-3], respectively, were isolated from an aqueous extract of the roots of Isatis indigotica. Racemic and scalemic mixtures of these enantiomers were separated by HPLC on a chiral semi-preparative column. Their structures including absolute configurations were determined by extensive spectroscopic analysis in conjunction with the calculation of electronic circular dichroism (ECD) spectra...
March 2016: Acta Pharmaceutica Sinica. B
Takeshi Kumagai, Yusuke Aratsu, Ryosuke Sugawara, Takamitsu Sasaki, Shinichi Miyairi, Kiyoshi Nagata
Ban-Lan-Gen is the common name for the dried roots of indigo plants, including Polygonum tinctorium, Isatis indigotica, Isatis tinctoria, and Strobilanthes cusia. Ban-Lan-Gen is frequently used as an anti-inflammatory and an anti-viral for the treatment of hepatitis, influenza, and various types of inflammation. One of the cytochrome P450 (CYP) enzymes, CYP3A4, is responsible for the metabolism of a wide variety of xenobiotics, including an estimated 60% of all clinically used drugs. In this study, we investigated the effect of Ban-Lan-Gen on the transcriptional activation of the CYP3A4 gene...
April 2016: Drug Metabolism and Pharmacokinetics
Weijiao Zhang, Xuexing Zheng, Nan Cheng, Weiwei Gai, Xianghong Xue, Yuxia Wang, Yuwei Gao, Junjie Shan, Songtao Yang, Xianzhu Xia
Adjuvants can enhance vaccine immunogenicity and induce long-term enhancement of immune responses. Thus, adjuvants are important for vaccine research. Polysaccharides isolated from select Chinese herbs have been demonstrated to possess various beneficial functions and excellent adjuvant abilities. In the present study, the polysaccharides IIP-A-1 and IIP-2 were isolated from Isatis indigotica root and compared with the common vaccine adjuvant aluminum hydroxide via intramuscular co-administration of inactivated rabies virus rCVS-11-G into mice...
June 2016: International Journal of Biological Macromolecules
Yu-Feng Liu, Ming-Hua Chen, Sheng Lin, Yu-Huan Li, Dan Zhang, Jian-Dong Jiang, Jian-Gong Shi
Five new indole alkaloid glucosides named isatindigotindolosides A-E (1-5), along with three known analogs (6-8), were isolated from an aqueous extract of the Isatis indigotica roots. Their structures including the absolute configurations were determined based on comprehensive spectroscopic data analysis, combined with chemical methods and electronic circular dichroism spectra calculations. In the preliminary assays, compounds 1, 6 and 7 showed antiviral activity against influenza virus A/Hanfang/359/95 (H3N2) with IC50 values of 14...
2016: Journal of Asian Natural Products Research
Ruibing Chen, Qing Li, Hexin Tan, Junfeng Chen, Ying Xiao, Ruifang Ma, Shouhong Gao, Philipp Zerbe, Wansheng Chen, Lei Zhang
Root and leaf tissue of Isatis indigotica shows notable anti-viral efficacy, and are widely used as "Banlangen" and "Daqingye" in traditional Chinese medicine. The plants' pharmacological activity is attributed to phenylpropanoids, especially a group of lignan metabolites. However, the biosynthesis of lignans in I. indigotica remains opaque. This study describes the discovery and analysis of biosynthetic genes and AP2/ERF-type transcription factors involved in lignan biosynthesis in I. indigotica. MeJA treatment revealed differential expression of three genes involved in phenylpropanoid backbone biosynthesis (IiPAL, IiC4H, Ii4CL), five genes involved in lignan biosynthesis (IiCAD, IiC3H, IiCCR, IiDIR, and IiPLR), and 112 putative AP2/ERF transcription factors...
2015: Frontiers in Plant Science
Lei Zhang, Junfeng Chen, Qing Li, Wansheng Chen
Jasmonates (JAs) act as conserved elicitors of plant secondary metabolism. JAs perception triggers extensive transcriptional reprogramming leading to activation of the entire metabolic pathways. The family of basic helix-loop-helix (bHLH) transcription factors (TFs) has essential roles in JA signaling; however, little is known about their roles in regulation of secondary metabolites in Isatis indigotica. In this study, we identified 78 putative IibHLH sequences using the annotation of I. indigotica transcriptome...
January 15, 2016: Gene
Jing Li, Beixian Zhou, Chufang Li, QiaoYan Chen, Yutao Wang, Zhengtu Li, Tingting Chen, Chunguang Yang, Zhihong Jiang, Nanshan Zhong, Zifeng Yang, Rongchang Chen
ETHNOPHARMACOLOGICAL RELEVANCE: Isatis indigotica is a traditional Chinese medicine. Its dried roots named "ban lan gen" in Chinese, are used for clinical treatment of virus infection, tumor, inflammation with a long history. However, its anti-influenza active ingredient and the underlying mechanism remain unclear. In this study, the anti-influenza and anti-inflammatory effects of a lignan glycoside: lariciresinol-4-O-β-D-glucopyranoside isolated from the root of I. indigotica on human alveolar epithelial cell line A549 infected with influenza A virus were investigated...
November 4, 2015: Journal of Ethnopharmacology
Raphael N Alolga, Yong Fan, Gang Zhang, Jin Li, Yi-Jing Zhao, Jimmy Lelu Kakila, Yan Chen, Ping Li, Lian-Wen Qi
K-601 is an herbal formulation for influenza consisting of Lonicera japonica, Isatis indigotica, Rheum palmatum, Phellodendron chinense, and Scutellaria baicalensis. In this work, we characterized the chemical constituents in K-601, identified the absorbed compounds and determined their pharmacokinetics in 6 Chinese and African volunteers by liquid chromatography with time-of-flight mass spectrometry. Similarity evaluation for chromatographic fingerprint of nine different batches showed values above 0.983. Totally, 50 components were identified in K-601...
2015: Scientific Reports
Ying Xiao, Qian Ji, Shouhong Gao, Hexin Tan, Ruibing Chen, Qing Li, Junfeng Chen, Yingbo Yang, Lei Zhang, Zhengtao Wang, Wansheng Chen, Zhibi Hu
A lignan, lariciresinol, is an important efficacious compound for the antiviral effect of Isatis indigotica, a widely used herb for the treatment of colds, fever, and influenza. Although some rate-limiting steps of the lariciresinol biosynthetic pathway are well known, the specific roles of gene family members in I. indigotica in regulating lariciresinol production are poorly understood. In the present study, a correlation analysis between the RNA sequencing (RNA-Seq) expression profile and lignan content by using I...
October 2015: Journal of Experimental Botany
Yu-Feng Liu, Ming-Hua Chen, Qing-Lan Guo, Sheng Lin, Cheng-Bo Xu, Yue-Ping Jiang, Yu-Huan Li, Jian-Dong Jiang, Jian-Gong Shi
Seven new glycosidic bisindole alkaloids, isatindigobisindolosides A-G (1-7), were isolated from an aqueous extract of the Isatis indigotica roots. Their structures including absolute configurations were determined by spectroscopic and chemical methods, together with calculations of electronic circular dichroism (ECD) spectra based on the quantum-mechanical time-dependent density functional theory. In the NMR spectra of 1-3, it is found that integration of H-2 and intensity of C-2 are affected not only by a substitution group at C-2 but also by solvents...
2015: Journal of Asian Natural Products Research
Guanzhen Gao, Qian Wang, Jianwu Zhou, Huiqin Wang, Lijing Ke, Pingfan Rao
Isatis indigotica Fort. (Chinese woad) has been widely used as a dye- and medicinal-plant in traditional Chinese medicine. Although I. indigotica has been cultivated in various regions across China in recent years, its innate immunity is little known. In this study, a protein with MW 37.5 kDa was purified from the extract of fresh Isatidis roots by SP-5PW cationic exchange and POROS HP2 hydrophobic interaction chromatography. The N-terminal amino acid sequence of the purified protein was subsequently determined as T-D-L-C-H-K-D-P-K-N-T-L-L by Edman degradation...
February 2015: Natural Product Communications
Yingying Zhou, Lei Kang, Shiying Liao, Qi Pan, Xianhong Ge, Zaiyun Li
The giant organs and enhanced concentrations of secondary metabolites realized by autopolyploidy are attractive for breeding the respective medicinal and agricultural plants and studying the genetic mechanisms. The traditional medicinal plant Chinese woad (Isatis indigotica Fort., 2n = 2x = 14) is now still largely used for the diseases caused by bacteria and viruses in China. In this study, its autopolyploids (3x, 4x) were produced and characterized together with the 2x donor for their phenotype and transcriptomic alterations by using high-throughput RNA sequencing...
2015: PloS One
Yan-Zhi Ma, Shao-Ying Ke
OBJECTIVE: To identify tetraploid Isatis indigotica strains through morphology and flow cytometry. METHODS: The tissue culture seedlings of tetraploid Isatis indigotica were root-tip squashed and chromosome counted before rooted climatized and transplanted in field. The plants in field were taken as experimental materials. Macroscopic observation was applied to identify by form and structure; Free-hand section was used to observe the length, width and density of stomas; And flow cytometry was applied to identify the ploidy...
May 2014: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
Juan-Juan Yang, Qiao-Sheng Guo, Su-Dan Chen, Qiao-Hua Deng
The experiment included three potassium levels (K0 0 g x kg(-1), K1 0.33 g x kg(-1), K2 0.67 g x kg(-1)) and two water gradients (well watered and drought stress), then measured growth indicators, SOD, POD, CAT activities and concents of osmotic regulation substances. To explore the effects of K fertilizer and water on growth and physiological characteristics of Isatis indigotica, providing reference for improving drought resistance of I. indigotica. The result showed drought stress inhibited the growth and decreased the biomass of I...
May 2014: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Xiaoqing Tang, Yunhua Xiao, Tingting Lv, Fangquan Wang, QianHao Zhu, Tianqing Zheng, Jie Yang
BACKGROUND: Isatis indigotica, the source of the traditional Chinese medicine Radix isatidis (Ban-Lan-Gen), is an extremely important economical crop in China. To facilitate biological, biochemical and molecular research on the medicinal chemicals in I. indigotica, here we report the first I. indigotica transcriptome generated by RNA sequencing (RNA-seq). RESULTS: RNA-seq library was created using RNA extracted from a mixed sample including leaf and root. A total of 33,238 unigenes were assembled from more than 28 million of high quality short reads...
2014: PloS One
Xiangyang Yuan, Liguang Zhang, Na Ning, Yinyuan Wen, Shuqi Dong, Meiqiang Yin, Meijun Guo, Binqiang Wang, Lei Feng, Pingyi Guo
Radix Isatidis (Isatis indigotica Fort.) is one of the most important traditional Chinese medicine plants. However, there is no suitable herbicide used for weed control in Radix Isatidis field during postemergence stage. To explore the safety of sulfonylurea herbicide nicosulfuron on Radix Isatidis (Isatis indigotica Fort.) seedlings and the photosynthetic physiological response of the plant to the herbicide, biological mass, leaf area, photosynthetic pigment content, photosynthetic rate, chlorophyll fluorescence characteristics, and P700 parameters of Radix Isatidis seedlings were analyzed 10 d after nicosulfuron treatment at 5th leaf stage in this greenhouse research...
2014: PloS One
Ping Chen, Ping Meng, Jin-Song Zhang, Chun-Xia He, Chang-Rong Jia, Jian-Zhong Li
Growth characteristics, stable carbon isotope discrimination (Delta13C), water use efficiency (WUE), and their correlation of Cassia obtusifolia and Isatis indigotica were measured at three soil water levels, i. e., 30%, 50% and 75% of field water holding capacity (FWHC), and at three growth stages. The growth indices of the two medicinal plants at 75% of FWHC were higher than those at 30% and 50% of FWHC, suggesting that the two medicinal plants could obtain high production under sufficient moisture condition...
May 2014: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
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