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isatis indigotica

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https://www.readbyqxmd.com/read/28702046/selection-and-validation-of-appropriate-reference-genes-for-qrt-pcr-analysis-in-isatis-indigotica-fort
#1
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants. Based on the Minimum Information for Publication of Real-Time Quantitative PCR Experiments (MIQE) guidelines, it is necessary to select and validate putative appropriate reference genes for qRT-PCR normalization. In the current study, three algorithms, geNorm, NormFinder, and BestKeeper, were applied to assess the expression stability of 10 candidate reference genes across five different tissues and three different abiotic stresses in Isatis indigotica Fort...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28540170/indole-alkaloid-sulfonic-acids-from-an-aqueous-extract-of-isatis-indigotica-roots-and-their-antiviral-activity
#2
Lingjie Meng, Qinglan Guo, Yufeng Liu, Minghua Chen, Yuhuan Li, Jiandong Jiang, Jiangong Shi
Six new indole alkaloid sulfonic acids (1-6), together with two analogues (7 and 8) that were previously reported as synthetic products, were isolated from an aqueous extract of the Isatis indigotica root. Their structures including the absolute configurations were determined by spectroscopic data analysis, combined with enzyme hydrolysis and comparison of experimental circular dichroism and calculated electronic circular dichroism spectra. In the preliminary assay, compounds 2 and 4 showed antiviral activity against Coxsackie virus B3 and influenza virus A/Hanfang/359/95 (H3N2), respectively...
May 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/28475367/diglycosidic-indole-alkaloid-derivatives-from-an-aqueous-extract-of-isatis-indigotica-roots
#3
Ling-Jie Meng, Qing-Lan Guo, Cheng-Bo Xu, Cheng-Gen Zhu, Yu-Feng Liu, Ming-Hua Chen, Sheng Lin, Yu-Huan Li, Jian-Dong Jiang, Jian-Gong Shi
Six new indole alkaloid diglycosides named isatigotindolediosides A-F (1-6), along with three known analogs (7-9), were isolated from an aqueous extract of the Isatis indigotica roots (ban lan gen). Their structures including the absolute configurations were determined by comprehensive spectroscopic data analysis, combined with enzyme or acid hydrolysis, and comparison of experimental circular dichroism (CD) and calculated electronic circular dichroism (ECD) spectra. In the preliminary assays, compounds 3, 5, and 8 showed antiviral activity against Coxsackie virus B3...
May 5, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28428799/a-novel-cytoplasmic-male-sterility-in-brassica-napus-inap-cms-with-carpelloid-stamens-via-protoplast-fusion-with-chinese-woad
#4
Lei Kang, Pengfei Li, Aifan Wang, Xianhong Ge, Zaiyun Li
A novel cytoplasmic male sterility (CMS) in Brassica napus (inap CMS) was selected from the somatic hybrid with Isatis indigotica (Chinese woad) by recurrent backcrossing. The male sterility was caused by the conversion of tetradynamous stamens into carpelloid structures with stigmatoid tissues at their tips and ovule-like tissues in the margins, and the two shorter stamens into filaments without anthers. The feminized development of the stamens resulted in the complete lack of pollen grains, which was stable in different years and environments...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28417088/boiling-induced-nanoparticles-and-their-constitutive-proteins-from-isatis-indigotica-fort-root-decoction-purification-and-identification
#5
Jianwu Zhou, Jie Liu, Dai Lin, Guanzhen Gao, Huiqin Wang, Jingke Guo, Pingfan Rao, Lijing Ke
Colloidal particles are essential components of sun-dried Isatis indigotica Fort. roots (Ban-Lan-Gen in Chinese, BLG) decoction. Nanoparticles (NPs) were isolated from BLG decoction with size exclusion chromatography and characterized. Their average diameter is ∼120 nm, reversibly responding to pH and temperature changes. They promoted the growth of normal cells but suppressed that of cancerogenic cells and macrophages. Two constitutive glycated proteins were identified from the NPs, namely BLGP1 and BLGP2...
April 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28303224/aromatic-compounds-from-an-aqueous-extract-of-ban-lan-gen-and-their-antiviral-activities
#6
Yufeng Liu, Minghua Chen, Qinglan Guo, Yuhuan Li, Jiandong Jiang, Jiangong Shi
A pair of new diphenyl glycerol ether enantiomers (-)-1 and (+)-1 and two new methyl benzamidobenzoates 2 and 3, named (-)-(R)- and (+)-(S)-isatindigotrioic acid [(-)-1 and (+)-1] and isatindigoticamides A (2) and B (3), respectively, were isolated from an aqueous decoction of the roots of Isatis indigotica (ban lan gen). Their structures were elucidated by spectroscopic data analysis including 2D NMR experiments. The absolute configurations of (-)-1 and (+)-1 were assigned based on the CD exciton chirality method...
March 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/28284151/simultaneous-decolorization-of-sulfonated-azo-dyes-and-reduction-of-hexavalent-chromium-under-high-salt-condition-by-a-newly-isolated-salt-tolerant-strain-bacillus-circulans-bwl1061
#7
Weijie Liu, Cong Liu, Liang Liu, Yanting You, Jihong Jiang, Zhengkun Zhou, Zhen Dong
The co-existence of dyes, Cr(VI) and high concentration of salt in dyeing wastewater causes serious and complex environmental problems. In this study, a salt-tolerant strain Bacillus circulans BWL1061 was reported to simultaneously remove 50mg/L methyl orange and 50mg/L Cr(VI) under the anaerobic condition with 60g/L NaCl. During the decolorization process, the Cr(VI) reduction occurred preferentially over the dye decolorization due to the dominate utilization of electron by Cr(VI). The analysis of enzyme activities suggested that azoreductase, NADH-DCIP reductase, and laccase were associated with decolorization of methyl orange...
March 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28273165/transcriptome-profiling-of-influenza-a-virus-infected-lung-epithelial-a549-cells-with-lariciresinol-4-%C3%AE-d-glucopyranoside-treatment
#8
Beixian Zhou, Jing Li, Xiaoli Liang, Zifeng Yang, Zhihong Jiang
The influenza A virus is an acute contagious pathogen that affects the human respiratory system and can cause severe lung disease and even death. Lariciresinol-4-β-D-glucopyranoside is a lignan that is extracted from Isatis indigotica, which is a medicinal herb plant that was commonly applied to treat infections, the common cold, fever and inflammatory diseases. Our previous study demonstrated that lariciresinol-4-β-D-glucopyranoside possesses anti-viral and anti-inflammatory properties. However, the comprehensive and detailed mechanisms that underlie the effect of lariciresinol-4-β-D-glucopyranoside interventions against influenza virus infection remain to be elucidated...
2017: PloS One
https://www.readbyqxmd.com/read/27914524/the-phenylalanine-ammonia-lyase-gene-family-in-isatis-indigotica-fort-molecular-cloning-characterization-and-expression-analysis
#9
Rui-Fang Ma, Qian-Zi Liu, Ying Xiao, Lei Zhang, Qing Li, Jun Yin, Wan-Sheng Chen
Phenolic compounds, metabolites of the phenylpropanoid pathway, play an important role in the growth and environmental adaptation of many plants. Phenylalanine ammonia-lyase (PAL) is the first key enzyme of the phenylpropanoid pathway. The present study was designed to investigate whether there is a multi-gene family in I. Indigotic and, if so, to characterize their properties. We conducted a comprehensive survey on the transcription profiling database by using tBLASTn analysis. Several bioinformatics methods were employed to perform the prediction of composition and physicochemical characters...
November 2016: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/27155368/dynamic-metabolic-and-transcriptomic-profiling-of-methyl-jasmonate-treated-hairy-roots-reveals-synthetic-characters-and-regulators-of-lignan-biosynthesis-in-isatis-indigotica-fort
#10
Lei Zhang, Junfeng Chen, Xun Zhou, Xiaofei Chen, Qing Li, Hexin Tan, Xin Dong, Ying Xiao, Langdong Chen, Wansheng Chen
A molecular description of lignan biosynthesis in Isatis indigotica displaying its synthetic characteristics and regulatory mechanism is of great importance for the improvement of the production of this class of active compounds. To discover the potential key catalytic steps and regulatory genes, I. indigotica hairy roots elicited by methyl jasmonate (MeJA) were used as a source of systematic variation for exploring the metabolic/transcriptional changes and candidate genes that might play key roles in lignan biosynthesis...
December 2016: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/27071247/-cloning-and-expression-analysis-of-a-hydroxycinnamoyl-coa-shikimate-quinate-hydroxycinnamoyl-transferasegene-iihct-from-isatis-indigotica
#11
Hong-ran Dong, Jian Yang, Lu-qi Huang, Jing-ming Jia, Jin-fu Tang
Based on the transcriptome data, we cloned the open reading frame of IiHCT gene from Isatis indigotica, and then performed bioinformatic analysis of the sequence. Further, we detected expression pattern in specific organs and hairy roots treated methyl jasmonate( MeJA) by RT-PCR. The IiHCT gene contains a 1 290 bp open reading frame( ORF) encoding a polypeptide of 430 amino acids. The predicted isoelectric point( pI) was 5.7, a calculated molecular weight was about 47.68 kDa. IiHCT was mainly expressed in stem and undetectable in young root, leaf and flower bud...
November 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/27025363/computational-identification-and-systematic-classification-of-novel-gras-genes-in-isatis-indigotica
#12
REVIEW
Lei Zhang, Qing Li, Jun-Feng Chen, Wan-Sheng Chen
Isatis indigotica Fort., belonging to Cruciferae, is one of the most commonly used plants in traditional Chinese medicine. The accumulation of the effective components of I. indigotica is related with its growth conditions. The GRAS genes are members of a multigene family of transcriptional regulators that play a crucial role in plant growth. Although the activities of many GRAS genes have long been recognized, only in recent years were some of them identified and functionally characterized in detail. In the present study, 41 GRAS genes were identified from I...
March 2016: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/27006898/three-pairs-of-alkaloid-enantiomers-from-the-root-of-isatis-indigotica
#13
Yufeng Liu, Xiaoliang Wang, Minghua Chen, Sheng Lin, Li Li, Jiangong Shi
Three pairs of enantiomerically pure alkaloids with diverse structure features, named isatindigoticoic acid A and epiisatindigoticoic acid A [(-)-1 and (+)-1], phaitanthrin A and epiphaitanthrin A [(-)-2 and (+)-2], and isatindopyrromizol A and epiisatindopyrromizol A [(-)-3 and (+)-3], respectively, were isolated from an aqueous extract of the roots of Isatis indigotica. Racemic and scalemic mixtures of these enantiomers were separated by HPLC on a chiral semi-preparative column. Their structures including absolute configurations were determined by extensive spectroscopic analysis in conjunction with the calculation of electronic circular dichroism (ECD) spectra...
March 2016: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/26987505/indirubin-a-component-of-ban-lan-gen-activates-cyp3a4-gene-transcription-through-the-human-pregnane-x-receptor
#14
Takeshi Kumagai, Yusuke Aratsu, Ryosuke Sugawara, Takamitsu Sasaki, Shinichi Miyairi, Kiyoshi Nagata
Ban-Lan-Gen is the common name for the dried roots of indigo plants, including Polygonum tinctorium, Isatis indigotica, Isatis tinctoria, and Strobilanthes cusia. Ban-Lan-Gen is frequently used as an anti-inflammatory and an anti-viral for the treatment of hepatitis, influenza, and various types of inflammation. One of the cytochrome P450 (CYP) enzymes, CYP3A4, is responsible for the metabolism of a wide variety of xenobiotics, including an estimated 60% of all clinically used drugs. In this study, we investigated the effect of Ban-Lan-Gen on the transcriptional activation of the CYP3A4 gene...
April 2016: Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/26875535/isatis-indigotica-root-polysaccharides-as-adjuvants-for-an-inactivated-rabies-virus-vaccine
#15
Weijiao Zhang, Xuexing Zheng, Nan Cheng, Weiwei Gai, Xianghong Xue, Yuxia Wang, Yuwei Gao, Junjie Shan, Songtao Yang, Xianzhu Xia
Adjuvants can enhance vaccine immunogenicity and induce long-term enhancement of immune responses. Thus, adjuvants are important for vaccine research. Polysaccharides isolated from select Chinese herbs have been demonstrated to possess various beneficial functions and excellent adjuvant abilities. In the present study, the polysaccharides IIP-A-1 and IIP-2 were isolated from Isatis indigotica root and compared with the common vaccine adjuvant aluminum hydroxide via intramuscular co-administration of inactivated rabies virus rCVS-11-G into mice...
June 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/26651370/indole-alkaloid-glucosides-from-the-roots-of-isatis-indigotica
#16
Yu-Feng Liu, Ming-Hua Chen, Sheng Lin, Yu-Huan Li, Dan Zhang, Jian-Dong Jiang, Jian-Gong Shi
Five new indole alkaloid glucosides named isatindigotindolosides A-E (1-5), along with three known analogs (6-8), were isolated from an aqueous extract of the Isatis indigotica roots. Their structures including the absolute configurations were determined based on comprehensive spectroscopic data analysis, combined with chemical methods and electronic circular dichroism spectra calculations. In the preliminary assays, compounds 1, 6 and 7 showed antiviral activity against influenza virus A/Hanfang/359/95 (H3N2) with IC50 values of 14...
2016: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/26579184/gene-to-metabolite-network-for-biosynthesis-of-lignans-in-meja-elicited-isatis-indigotica-hairy-root-cultures
#17
Ruibing Chen, Qing Li, Hexin Tan, Junfeng Chen, Ying Xiao, Ruifang Ma, Shouhong Gao, Philipp Zerbe, Wansheng Chen, Lei Zhang
Root and leaf tissue of Isatis indigotica shows notable anti-viral efficacy, and are widely used as "Banlangen" and "Daqingye" in traditional Chinese medicine. The plants' pharmacological activity is attributed to phenylpropanoids, especially a group of lignan metabolites. However, the biosynthesis of lignans in I. indigotica remains opaque. This study describes the discovery and analysis of biosynthetic genes and AP2/ERF-type transcription factors involved in lignan biosynthesis in I. indigotica. MeJA treatment revealed differential expression of three genes involved in phenylpropanoid backbone biosynthesis (IiPAL, IiC4H, Ii4CL), five genes involved in lignan biosynthesis (IiCAD, IiC3H, IiCCR, IiDIR, and IiPLR), and 112 putative AP2/ERF transcription factors...
2015: Frontiers in Plant Science
https://www.readbyqxmd.com/read/26449398/transcriptome-wide-analysis-of-basic-helix-loop-helix-transcription-factors-in-isatis-indigotica-and-their-methyl-jasmonate-responsive-expression-profiling
#18
Lei Zhang, Junfeng Chen, Qing Li, Wansheng Chen
Jasmonates (JAs) act as conserved elicitors of plant secondary metabolism. JAs perception triggers extensive transcriptional reprogramming leading to activation of the entire metabolic pathways. The family of basic helix-loop-helix (bHLH) transcription factors (TFs) has essential roles in JA signaling; however, little is known about their roles in regulation of secondary metabolites in Isatis indigotica. In this study, we identified 78 putative IibHLH sequences using the annotation of I. indigotica transcriptome...
January 15, 2016: Gene
https://www.readbyqxmd.com/read/26320688/lariciresinol-4-o-%C3%AE-d-glucopyranoside-from-the-root-of-isatis-indigotica-inhibits-influenza-a-virus-induced-pro-inflammatory-response
#19
Jing Li, Beixian Zhou, Chufang Li, QiaoYan Chen, Yutao Wang, Zhengtu Li, Tingting Chen, Chunguang Yang, Zhihong Jiang, Nanshan Zhong, Zifeng Yang, Rongchang Chen
ETHNOPHARMACOLOGICAL RELEVANCE: Isatis indigotica is a traditional Chinese medicine. Its dried roots named "ban lan gen" in Chinese, are used for clinical treatment of virus infection, tumor, inflammation with a long history. However, its anti-influenza active ingredient and the underlying mechanism remain unclear. In this study, the anti-influenza and anti-inflammatory effects of a lignan glycoside: lariciresinol-4-O-β-D-glucopyranoside isolated from the root of I. indigotica on human alveolar epithelial cell line A549 infected with influenza A virus were investigated...
November 4, 2015: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/26268432/pharmacokinetics-of-a-multicomponent-herbal-preparation-in-healthy-chinese-and-african-volunteers
#20
Raphael N Alolga, Yong Fan, Gang Zhang, Jin Li, Yi-Jing Zhao, Jimmy Lelu Kakila, Yan Chen, Ping Li, Lian-Wen Qi
K-601 is an herbal formulation for influenza consisting of Lonicera japonica, Isatis indigotica, Rheum palmatum, Phellodendron chinense, and Scutellaria baicalensis. In this work, we characterized the chemical constituents in K-601, identified the absorbed compounds and determined their pharmacokinetics in 6 Chinese and African volunteers by liquid chromatography with time-of-flight mass spectrometry. Similarity evaluation for chromatographic fingerprint of nine different batches showed values above 0.983. Totally, 50 components were identified in K-601...
2015: Scientific Reports
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