keyword
MENU ▼
Read by QxMD icon Read
search

virus neutralisation

keyword
https://www.readbyqxmd.com/read/28533093/safety-and-immunogenicity-of-a-live-attenuated-influenza-h5-candidate-vaccine-strain-a-17-turkey-turkey-05-133-h5n2-and-its-priming-effects-for-potential-pre-pandemic-use-a-randomised-double-blind-placebo-controlled-trial
#1
Punnee Pitisuttithum, Kobporn Boonnak, Supat Chamnanchanunt, Pilaipan Puthavathana, Viravarn Luvira, Hatairat Lerdsamran, Jaranit Kaewkungwal, Saranath Lawpoolsri, Vipa Thanachartwet, Udomsak Silachamroon, Wanibtisam Masamae, Alexandra Schuetz, Ponthip Wirachwong, Sit Thirapakpoomanunt, Larisa Rudenko, Erin Sparrow, Martin Friede, Marie-Paule Kieny
BACKGROUND: The emergence of highly pathogenic avian influenza H5N1 viruses has raised concerns about their pandemic potential. Vaccination is the most effective way of preventing influenza. In this study, we investigated the safety and immunogenicity of an avian H5N2 live attenuated influenza vaccine (LAIV H5N2) in healthy Thai adults and its priming immune responses with an H5N1 inactivated vaccine boost. METHODS: This study was done at the Vaccine Trial Centre at Mahidol University, Bangkok, Thailand and was divided into two parts...
May 19, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28507200/effects-of-microvirin-mono-and-oligomers-on-hepatitis-c-virus
#2
Yuan Qin Min, Xu Chu Duan, Yi Dan Zhou, Anna Kulinich, Meng Wang, Zhi-Peng Cai, Hong Yu Ma, Li Liu, Xiao Lian Zhang, Josef Voglmeir
Microvirin is a carbohydrate binding protein which shows high specificity for high-mannose type N-glycan structures. In this study we tried to identify whether microvirin could bind to high-mannose containing Hepatitis C Virus (HCV) envelope glycoproteins, which are heavily decorated decorated high-mannose glycans. In addition, recombinantly expressed microvirin oligomers in di-, tri-, and tetrameric form were evaluated for their viral inhibition. Microvirin oligomers bound more efficiently to HCV virions, and displayed in comparison to the microvirin monomer a higher neutralisation potency against HCV infection...
May 15, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28475122/harnessing-local-immunity-for-an-effective-universal-swine-influenza-vaccine
#3
REVIEW
Elma Tchilian, Barbara Holzer
Influenza A virus infections are a global health threat to humans and are endemic in pigs, contributing to decreased weight gain and suboptimal reproductive performance. Pigs are also a source of new viruses of mixed swine, avian, and human origin, potentially capable of initiating human pandemics. Current inactivated vaccines induce neutralising antibody against the immunising strain but rapid escape occurs through antigenic drift of the surface glycoproteins. However, it is known that prior infection provides a degree of cross-protective immunity mediated by cellular immune mechanisms directed at the more conserved internal viral proteins...
May 5, 2017: Viruses
https://www.readbyqxmd.com/read/28446669/salivary-blockade-protects-the-lower-respiratory-tract-of-mice-from-lethal-influenza
#4
Karen Ivinson, Georgia Deliyannis, Leanne McNabb, Lara Grollo, Brad Gilbertson, David Jackson, Lorena E Brown
It is possible to model progression of influenza virus from the upper respiratory tract to the lower respiratory tract in the mouse using viral inoculum delivered in a restricted manner to the nose. In this model, infection with the A/Udorn/307/72 (Udorn) strain of virus results ultimately in high viral titres in both the trachea and lungs. In contrast, the A/Puerto Rico/8/34 (PR8) strain causes an infection that is almost entirely limited to the nasal passages. The factors that govern the progression of virus down the respiratory tract are not well understood...
April 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28438410/vaccination-with-recombinant-modified-vaccinia-ankara-mva-viruses-expressing-single-african-horse-sickness-virus-vp2-antigens-induced-cross-reactive-virus-neutralising-antibodies-vnab-in-horses-when-administered-in-combination
#5
Nicola Mary Manning, Katarzyna Bachanek-Bankowska, Peter Paul Clement Mertens, Javier Castillo-Olivares
African horse sickness is a lethal viral disease of equids transmitted by biting midges of the Genus Culicoides. The disease is endemic to sub-Saharan Africa but outbreaks of high mortality and economic impact have occurred in the past in non-endemic regions of Africa, Asia and Southern Europe. Vaccination is critical for the control of this disease but only live attenuated vaccines are currently available. However, there are bio-safety concerns over the use of this type of vaccines, especially in non-endemic countries, and live attenuated vaccines do not have DIVA (Differentiation of Infected from Vaccinated Animals) capacity...
April 21, 2017: Vaccine
https://www.readbyqxmd.com/read/28434129/phage-displayed-peptides-that-mimic-epitopes-of-hepatitis-e-virus-capsid
#6
Osmany Larralde, Juraj Petrik
Hepatitis E is an emerging zoonotic infection of increasing public health threat for the UK, especially for immunosuppressed individuals. A human recombinant vaccine has been licensed only in China and is not clear whether it protects against hepatitis E virus (HEV) genotype 3, the most prevalent in Europe. The aim of this study was to use phage display technology as a tool to identify peptides that mimic epitopes of HEV capsid (mimotopes). We identified putative linear and conformational mimotopes using sera from Scottish blood donors that have the immunological imprint of past HEV infection...
April 22, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28421694/molecular-engineering-and-plant-expression-of-an-immunoglobulin-heavy-chain-scaffold-for-delivery-of-a-dengue-vaccine-candidate
#7
Mi-Young Kim, Craig Van Dolleweerd, Alastair Copland, Matthew John Paul, Sven Hofmann, Gina R Webster, Emily Julik, Ivonne Ceballos-Olvera, Jorge Reyes-Del Valle, Moon-Sik Yang, Yong-Suk Jang, Rajko Reljic, Julian K Ma
In order to enhance vaccine uptake by the immune cells in vivo, molecular engineering approach was employed to construct a Polymeric Immunoglobulin G Scaffold (PIGS) that incorporates multiple copies of an antigen and targets the Fc gamma receptors on antigen-presenting cells. These self-adjuvanting immunogens were tested in the context of dengue infection, for which there is currently no globally licensed vaccine yet. Thus, the consensus domain III sequence (cEDIII) of dengue glycoprotein E was incorporated into PIGS and expressed in both tobacco plants and Chinese Ovary Hamster cells...
April 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28406752/comparison-of-the-immunogenicity-and-safety-of-the-purified-chick-embryo-cell-rabies-vaccine-manufactured-in-india-and-germany-a-randomised-single-blind-multicentre-phase-iv-clinical-study
#8
Gadey Sampath, Angelika Banzhoff, Alaka Deshpande, Claudius Malerczyk, Ashwani Kumar Arora, Hoshang Vakil, Scott Preiss
This phase IV, single blind study assessed the immunogenicity and safety of India-manufactured purified chick embryo cell rabies vaccine (PCECV), compared to a German-manufactured batch obtained by the same production process. A total of 340 participants enrolled at 2 study sites in India were randomised (1:1:1:1) in 4 groups to receive a 5-dose Essen regimen with either 1 of the 3 Indian batches (PCECV-I) or the German batch (PCECV-G), administered on Days (D) 0, 3, 7, 14 and 30. The lot-to-lot consistency of PCECV-I batches in terms of induced immune response at D14 was demonstrated...
April 13, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28399176/pathological-findings-in-the-red-fox-vulpes-vulpes-stone-marten-martes-foina-and-raccoon-dog-nyctereutes-procyonoides-with-special-emphasis-on-infectious-and-zoonotic-agents-in-northern-germany
#9
Charlotte Lempp, Nicole Jungwirth, Miguel L Grilo, Anja Reckendorf, Arlena Ulrich, Abbo van Neer, Rogier Bodewes, Vanessa M Pfankuche, Christian Bauer, Albert D M E Osterhaus, Wolfgang Baumgärtner, Ursula Siebert
Anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. Hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. The present study provides a detailed characterisation of the health status of the red fox (Vulpes vulpes), stone marten (Martes foina) and raccoon dog (Nyctereutes procyonoides) in their natural rural and peri-urban habitats in Schleswig-Holstein, Germany between November 2013 and January 2016 with focus on zoonoses and infectious diseases that are potentially threatening to other wildlife or domestic animal species...
2017: PloS One
https://www.readbyqxmd.com/read/28397863/structure-of-the-ebola-virus-glycoprotein-spike-within-the-virion-envelope-at-11%C3%A2-%C3%A3-resolution
#10
Daniel R Beniac, Timothy F Booth
We present the structure of the surface Ebola virus (EBOV) trimeric glycoprotein (GP) spike at 11 Å resolution, in situ within the viral plasma membrane of purified virus particles. GP functions in cellular attachment, endosomal entry, and membrane fusion to initiate infection, and is a key therapeutic target. Nevertheless, only about half of the GP molecule has yet been solved to atomic resolution, excluding the mucin-like and transmembrane domains, and some of the glycans. Fitting of the atomic resolution X-ray data from expressed, truncated deletion constructs within our 11 Å structure of the entire molecule demonstrates the relationship between the GP1-GP2 domains, the mucin-like and transmembrane domains, and the bilaminar lipid envelope...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28397519/emerging-vector-borne-diseases-in-dromedaries-in-tunisia-west-nile-bluetongue-epizootic-haemorrhagic-disease-and-rift-valley-fever
#11
Thameur B Hassine, Jihane Amdouni, Federica Monaco, Giovanni Savini, Soufien Sghaier, Imed B Selimen, Walid Chandoul, Khaled B Hamida, Salah Hammami
A total of 118 sera were collected during 2016 from two groups of dromedaries from Kebili and Medenine governorates in the south of Tunisia. The aim of this study was to provide the first serological investigation of four emerging vector-borne diseases in two groups of dromedaries in Tunisia. Sera were tested by ELISA and serum neutralisation test to identify West Nile virus (WNV), bluetongue virus (BTV), epizootic haemorrhagic disease virus (EHDV) and Rift Valley fever virus (RVFV). In the first group, the seroprevalence for BTV was 4...
March 31, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/28397517/lumpy-skin-disease-in-cattle-frequency-of-occurrence-in-a-dairy-farm-and-a-preliminary-assessment-of-its-possible-impact-on-egyptian-buffaloes
#12
Mahmoud M Elhaig, Abdelfattah Selim, Mohamed Mahmoud
Lumpy skin disease (LSD) is an endemic infectious disease of cattle in Egypt. This survey aimed to define the prevalence of clinical and sub-clinical LSD virus (LSDV) infection among cattle and investigate their contact with water buffaloes (Bubalus bubalis) in order to improve the understanding of LSD epidemiology. Cattle and buffalo were examined owing to the appearance of skin lesions. Because clinical signs were consistent with LSDV infection, samples from cattle in a non-grazing dairy farm (n = 450) were submitted for LSDV testing together with those from the in-contact buffaloes (n = 100)...
March 28, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/28396205/entry-inhibition-of-hsv-1-and-2-protects-mice-from-viral-lethal-challenge
#13
Nicola Clementi, Elena Criscuolo, Francesca Cappelletti, Paola Quaranta, Mauro Pistello, Roberta A Diotti, Giuseppe A Sautto, Alexander W Tarr, Federico Mailland, Daniela Concas, Roberto Burioni, Massimo Clementi, Nicasio Mancini
The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed...
April 8, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28389099/a-multi-national-european-cross-sectional-study-of-feline-calicivirus-epidemiology-diversity-and-vaccine-cross-reactivity
#14
Maria M Afonso, Gina L Pinchbeck, Shirley L Smith, Janet M Daly, Rosalind M Gaskell, Susan Dawson, Alan D Radford
BACKGROUND: Feline calicivirus (FCV) is an important pathogen of cats for which vaccination is regularly practised. Long-term use of established vaccine antigens raises the theoretical possibility that field viruses could become resistant. This study aimed to assess the current ability of the FCV-F9 vaccine strain to neutralise a randomly collected contemporary panel of FCV field strains collected prospectively in six European countries. METHODS: Veterinary practices (64) were randomly selected from six countries (UK, Sweden, Netherlands, Germany, France and Italy)...
May 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28369886/efficacy-of-feline-anti-parvovirus-antibodies-in-the-treatment-of-canine-parvovirus-infection
#15
M Gerlach, A L Proksch, S Unterer, S Speck, U Truyen, K Hartmann
OBJECTIVE: This prospective, randomised, placebo-controlled, double-blinded study aimed to evaluate efficacy of commercially available feline anti-parvovirus antibodies in dogs with canine parvovirus infection. METHODS: First, cross-protection of feline panleukopenia virus antibodies against canine parvovirus was evaluated in vitro. In the subsequent prospective clinical trial, 31 dogs with clinical signs of canine parvovirus infection and a positive faecal canine parvovirus polymerase chain reaction were randomly assigned to a group receiving feline panleukopenia virus antibodies (n=15) or placebo (n=16)...
March 31, 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28365225/safety-and-immunogenicity-of-one-versus-two-doses-of-takeda-s-tetravalent-dengue-vaccine-in-children-in-asia-and-latin-america-interim-results-from-a-phase-2-randomised-placebo-controlled-study
#16
Xavier Sáez-Llorens, Vianney Tricou, Delia Yu, Luis Rivera, Suely Tuboi, Pedro Garbes, Astrid Borkowski, Derek Wallace
BACKGROUND: Dengue is the most common mosquito-borne viral disease in human beings, and vector control has not halted its spread worldwide. A dengue vaccine for individuals aged 9 years and older has been licensed, but there remains urgent medical need for a vaccine that is safe and effective against all four dengue virus serotypes (DENV-1-4) in recipients of all ages. Here, we present the preplanned interim analyses at 6 months of a tetravalent dengue vaccine candidate (TDV), which is comprised of an attenuated DENV-2 virus strain (TDV-2) and three chimeric viruses containing the premembrane and envelope protein genes of DENV-1, DENV-3, and DENV-4 genetically engineered into the attenuated TDV-2 genome backbone (TDV-1, TDV-3, and TDV-4)...
March 29, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28348774/latent-infection-of-human-bocavirus-accompanied-by-flare-of-chronic-cough-fatigue-and-episodes-of-viral-replication-in-an-immunocompetent-adult-patient-cologne-germany
#17
Wolfram Windisch, Monika Pieper, Inga Ziemele, Jürgen Rockstroh, Michael Brockmann, Oliver Schildgen, Verena Schildgen
INTRODUCTION: The human bocavirus (HBoV) is a parvovirus and is associated with mild to life-threatening acute or persisting respiratory infections, frequently accompanied by further pathogens. So far, there is limited knowledge on the mechanisms of persistence, and no reports on chronic infections or latency have been published so far. CASE PRESENTATION: An immunocompetent male patient suffers from a chronic HBoV1 infection, i.e. viral DNA was detected in both serum and bronchoalveolar lavage (BAL) for >5 months without co-infections and with respiratory symptoms resolved spontaneously while receiving symptomatic treatment with montelukast and corticosteroids...
August 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28344163/characterization-of-antibody-response-in-neuroinvasive-infection-caused-by-toscana-virus
#18
Anna Pierro, Sabrina Ficarelli, Nazli Ayhan, Silvia Morini, Luigi Raumer, Michele Bartoletti, Antonio Mastroianni, Francesca Prati, Simona Schivazappa, Patrizia Cenni, Caterina Vocale, Giada Rossini, Paolo Gaibani, Vittorio Sambri, Maria Paola Landini, Russel Edward Lewis, Remi N Charrel, Stefania Varani
OBJECTIVES: Among sandfly-borne pathogens, Toscana virus (TOSV) is a prominent cause of summer meningitis in Mediterranean Europe. Here, we assessed the kinetics of anti-TOSV antibodies over time in 41 patients diagnosed with TOSV-meningitis or meningoencephalitis in North-eastern Italy. METHODS: Acute and follow-up serum samples were collected up to 20 months after diagnosis of TOSV infection and tested for the presence of specific antibody using immunoenzymatic and indirect immunofluorescence assays...
March 23, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28341113/dynamics-of-african-horse-sickness-virus-nucleic-acid-and-antibody-in-horses-following-immunization-with-a-commercial-polyvalent-live-attenuated-vaccine
#19
C T Weyer, J D Grewar, P Burger, C Joone, C Lourens, N J MacLachlan, A J Guthrie
African horse sickness (AHS) is a fatal disease of equids relevant to the global equine industry. Detection of AHS virus (AHSV) during outbreaks has become more rapid and efficient with the advent of group specific reverse transcriptase quantitative polymerase chain reaction (GS RT-qPCR) assays to detect AHSV nucleic acid. Use of GS RT-qPCR together with recently described type specific (TS RT-qPCR) assays cannot only expedite diagnosis of AHS but also facilitate further evaluation of the dynamics of AHSV infection in the equine host...
March 21, 2017: Vaccine
https://www.readbyqxmd.com/read/28333616/cross-sectional-surveillance-of-middle-east-respiratory-syndrome-coronavirus-mers-cov-in-dromedary-camels-and-other-mammals-in-egypt-august-2015-to-january-2016
#20
Mohamed Ali, Rabeh El-Shesheny, Ahmed Kandeil, Mahmoud Shehata, Basma Elsokary, Mokhtar Gomaa, Naglaa Hassan, Ahmed El Sayed, Ahmed El-Taweel, Heba Sobhy, Folorunso Oludayo Fasina, Gwenaelle Dauphin, Ihab El Masry, Abebe Wossene Wolde, Peter Daszak, Maureen Miller, Sophie VonDobschuetz, Subhash Morzaria, Juan Lubroth, Yilma Jobre Makonnen
A cross-sectional study was conducted in Egypt to determine the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) in imported and resident camels and bats, as well as to assess possible transmission of the virus to domestic ruminants and equines. A total of 1,031 sera, 1,078 nasal swabs, 13 rectal swabs, and 38 milk samples were collected from 1,078 camels in different types of sites. In addition, 145 domestic animals and 109 bats were sampled. Overall, of 1,031 serologically-tested camels, 871 (84...
March 16, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
keyword
keyword
120066
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"