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plate rich plasma

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https://www.readbyqxmd.com/read/29146168/bone-marrow-derived-mesenchymal-stromal-cells-enhanced-by-platelet-rich-plasma-maintain-adhesion-to-scaffolds-in-arthroscopic-simulation
#1
Alexander R Hoberman, Carl Cirino, Mary Beth McCarthy, Mark P Cote, Leo Pauzenberger, Knut Beitzel, Augustus D Mazzocca, Felix Dyrna
PURPOSE: To assess the response of bone marrow-derived mesenchymal stromal cells (bMSCs) enhanced by platelet-rich plasma (PRP) in the setting of a normal human tendon (NHT), a demineralized bone matrix (DBM), and a fibrin scaffold (FS) with simulated arthroscopic mechanical washout stress. METHODS: Bone marrow was aspirated from the humeral head and concentrated. BMSCs were counted, plated, and grown to confluence. Cells were seeded onto 3 different scaffolds: (1) NHT, (2) DBM, and (3) FS...
November 13, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29023589/light-transmission-aggregometry-using-pre-coated-microtiter-plates-and-a-victor-x5-plate-reader
#2
Pernille Just Vinholt, Mads Nybo, Camilla Brødsgaard Nielsen, Anne-Mette Hvas
BACKGROUND: Light transmission aggregometry (LTA) can be performed with microtiter plates (96-well LTA). When conducting LTA, an agonist is added to platelet-rich plasma and the sample is shaken for minutes after which absorbance readings are done. Platelet aggregation is detected as decrease in absorbance. However, the classical method is cumbersome and therefore microtiter plates can be used for concomitant testing of multiple samples. Furthermore, it would be convenient to prepare the plate in advance of platelet aggregation testing...
2017: PloS One
https://www.readbyqxmd.com/read/28884628/the-effect-of-tannic-acid-on-bone-mechanical-and-geometric-properties-bone-density-and-trabecular-histomorphometry-as-well-as-the-morphology-of-articular-and-growth-cartilages-in-rats-co-exposed-to-cadmium-and-lead-is-dose-dependent
#3
Ewa Tomaszewska, Piotr Dobrowolski, Anna Winiarska-Mieczan, Małgorzata Kwiecień, Siemowit Muszyński, Agnieszka Tomczyk
Cadmium (Cd) and lead (Pb) are toxic elements that accumulate to the largest extent in bones. Rats were used to investigate whether tannic acid (TA; 0.5%, 1.0%, 1.5%. 2.0%, or 2.5%) would have a protective effect on the structure and properties of bones in the case of exposure to Cd and Pb (diet: 7 mg Cd/kg and 50 mg Pb/kg) for 6 weeks. The effects of administration of TA in Cd- and Pb-poisoned rats on bone characteristics and the morphology of articular and growth cartilages were determined. All the rats administered Cd and Pb had an enhanced Cd and Pb concentration in blood plasma and bone and reduced bone Ca content irrespective of the TA administration...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/28827850/platelet-poor-plasma-as-a-supplement-for-fibroblasts-cultured-in-platelet-rich-fibrin
#4
Luiz Alexandre Chisini, Sarah Arangurem Karam, Thaís Gioda Noronha, Letícia Regina Morello Sartori, Alissa Schmidt San Martin, Flávio Fernando Demarco, Marcus Cristian Muniz Conde
The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco's Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)(2) to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x10(4) cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days...
June 2017: Acta Stomatologica Croatica
https://www.readbyqxmd.com/read/28823058/polyurethane-gelatin-nanofibrils-neural-guidance-conduit-containing-platelet-rich-plasma-and-melatonin-for-transplantation-of-schwann-cells
#5
Majid Salehi, Mahdi Naseri-Nosar, Somayeh Ebrahimi-Barough, Mohammdreza Nourani, Arash Khojasteh, Saeed Farzamfar, Korosh Mansouri, Jafar Ai
The current study aimed to enhance the efficacy of peripheral nerve regeneration using a biodegradable porous neural guidance conduit as a carrier to transplant allogeneic Schwann cells (SCs). The conduit was prepared from polyurethane (PU) and gelatin nanofibrils (GNFs) using thermally induced phase separation technique and filled with melatonin (MLT) and platelet-rich plasma (PRP). The prepared conduit had the porosity of 87.17 ± 1.89%, the contact angle of 78.17 ± 5.30° and the ultimate tensile strength and Young's modulus of 5...
August 19, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28667397/delayed-union-of-humeral-shaft-fractures-comparison-of-autograft-with-and-without-platelet-rich-plasma-treatment-a-randomized-single-blinded-clinical-trial
#6
Carlos Acosta-Olivo, Abiel Garza-Borjon, Mario Simental-Mendia, Felix Vilchez-Cavazos, Yadira Tamez-Mata, Victor Peña-Martinez
INTRODUCTION: Despite an adequate orthopedic treatment with functional bracing, some patients develop a delayed union in humeral shaft fractures. The objective of the present study was to determine the bone consolidation time among patients with delayed union of diaphyseal humeral fractures who were managed with locking compression plate (LCP) fixation combined with an iliac crest autograft using platelet-rich plasma (PRP) as a co-adjuvant. MATERIALS AND METHODS: This study was a controlled, randomized, experimental, longitudinal, comparative, prospective, blind clinical trial...
June 30, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28532001/apatite-grown-in-niobium-by-two-step-plasma-electrolytic-oxidation
#7
Bruno Leandro Pereira, Carlos Maurício Lepienski, Irineu Mazzaro, Neide Kazue Kuromoto
Plasma electrolytic oxidation (PEO) of niobium plates were done electrochemically in two steps with electrolytes containing phosphorous and calcium being observed the formation of crystalline apatite. All samples were submitted to a first step of PEO using an electrolyte containing phosphate ions. The second oxidization step was made using three different electrolytes. Some samples were oxidized by PEO in electrolyte containing calcium, while in other samples it was used two mixtures of phosphoric acid and calcium acetate monohydrate solutions...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28524398/anti-inflammatory-and-analgesic-activities-of-terminalia-muelleri-benth-combretaceae
#8
Nouran M Fahmy, Eman Al-Sayed, Mohamed M Abdel-Daim, Abdel Nasser Singab
Preclinical Research The anti-inflammatory and analgesic activities of a polyphenol-rich fraction (TMEF) obtained from Terminalia muelleri Benth. were measured. The analgesic activity of TMEF was tested using acetic acid-induced writhing and hot plate models in mice. The anti-inflammatory activity was assessed using carrageenan-induced paw edema model by measuring PGE2 , TNF-α, IL-1β, and IL-6 plasma levels as well as the paw thickness. TMEF was tested at doses of 100, 200, and 400 mg/kg p.o. and diclofenac sodium was used as a standard (100 mg/kg) in all experiments...
May 19, 2017: Drug Development Research
https://www.readbyqxmd.com/read/28296142/platelet-rich-plasma-for-androgenetic-alopecia-does-it-work-evidence-from-meta-analysis
#9
REVIEW
Salvatore Giordano, Marco Romeo, Petteri Lankinen
INTRODUCTION: The use of platelet-rich plasma (PRP) has increased among different surgical specialities for the treatment of various conditions. Androgenetic alopecia is a common condition, with severe attendant psychosocial implications. PRP injections for hair restoration have become a popular practice among plastic surgeons. We performed a meta-analysis comparing local injection of platelet-rich plasma versus control to evaluate this issue in order to investigate the effectiveness of PRP local injections for androgenetic alopecia...
March 13, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28236247/humeral-shaft-non-union-after-intramedullary-nailing
#10
G Campochiaro, P Baudi, M Gialdini, A Corradini, V Duca, M Rebuzzi, F Catani
BACKGROUND: The humerus shaft is one of the sites with the largest probability of developing pseudoarthrosis after fracture. We present the results of nine patients with atrophic pseudoarthrosis of humeral shaft treated with angular stability plate associated with allograft and platelet-rich plasma (PRP), after a first treatment with intramedullary nail to correct the fracture. MATERIAL AND METHODS: From January 2012 to December 2014, nine patients were treated for atrophic pseudoarthrosis (PSA) of humeral shaft treated previously using intramedullary nail; seven humeral diaphysis fractures were located in the middle-proximal third and two in the middle third...
August 2017: Musculoskeletal Surgery
https://www.readbyqxmd.com/read/28190827/use-of-autologous-platelet-rich-plasma-for-a-delayed-consolidation-of-a-tibial-fracture-in-a-young-donkey
#11
Vanessa Faillace, Adolfo Maria Tambella, Margherita Fratini, Emanuele Paggi, Fabrizio Dini, Fulvio Laus
A 9-month-old filly donkey was referred for a comminuted diaphyseal fracture of the right tibia. Surgical osteosynthesis, with multiple lag screws and a neutralization plate, was performed for anatomical reconstruction. Despite a good gait condition, delayed bone consolidation and a bone gap were evident on follow up radiographic evaluations. Due to delayed healing, autologous platelet-rich plasma (PRP) was injected on the surface of the tibia. Increased bone consolidation was evident on radiographs one month after the PRP injection...
March 23, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28042260/effect-of-platelet-rich-plasma-concentrations-on-the-proliferation-of-periodontal-cells-an-in-vitro-study
#12
Sara Tavassoli-Hojjati, Mandana Sattari, Tayebeh Ghasemi, Rahil Ahmadi, Abbas Mashayekhi
OBJECTIVE: The purpose of this study was to evaluate the effect of different concentrations of platelet-rich plasma (PRP) on the proliferation of undifferentiated periodontal ligament (PDL) fibroblasts. MATERIALS AND METHODS: The undifferentiated PDL fibroblasts were obtained from two healthy human premolar teeth and cultured in Dulbecco's modified Eagle's medium. Cell wells were divided into five groups. Experimental groups received 0.1%, 5%, or 50% PRP; the positive and negative control groups were cultured in fetal bovine serum (FBS) 12% and in a medium without FBS 12%, respectively...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/27994625/effect-of-mesenchymal-stem-cells-and-platelet-rich-plasma-on-the-bone-healing-of-ovariectomized-rats
#13
Bo Wei, Chengshuo Huang, Mingyan Zhao, Peng Li, Xiang Gao, Junchao Kong, Yanru Niu, Rui Huang, Juanhua Quan, Jinsong Wei, Jiaqi Chu
We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27989570/the-effect-of-tannic-acid-on-the-bone-tissue-of-adult-male-wistar-rats-exposed-to-cadmium-and-lead
#14
Ewa Tomaszewska, Piotr Dobrowolski, Anna Winiarska-Mieczan, Małgorzata Kwiecień, Agnieszka Tomczyk, Siemowit Muszyński
Toxic elements such as cadmium (Cd) and lead (Pb) accumulate to the largest extent in bones. Rats at the age of 12 weeks were used to check whether tannic acid (TA) at the concentration of 0.5%, 1.0%, 1.5%. 2.0% or 2.5% would have a protective effect on the structure and properties of bones in the case of exposure to Cd and Pb (diet: 7mg Cd/kg and 50mg Pb/kg) for 12 weeks. The effects of administration of TA in Cd- and Pb-poisoned rats on bone mechanical and geometric properties, trabecular histomorphometry as well as the morphology of articular and growth cartilages were determined...
March 2, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/27867268/composition-of-the-essential-oil-from-danggui-zhiqiao-herb-pair-and-its-analgesic-activity-and-effect-on-hemorheology-in-rats-with-blood-stasis-syndrome
#15
Yuanqing Wang, Jianye Yan, Shunxiang Li, Wei Wang, Xiong Cai, Dan Huang, Limin Gong, Xin Li
BACKGROUND: Angelica sinensis and Aurantii fructu used in a pair, named Danggui-Zhiqiao herb-pair (DZHP), which was rich in essential oil and has been adopted to promote blood circulation, dispel blood stasis, and relieve pain in traditional Chinese medicine (TCM). OBJECTIVE: To analyze the composition and pharmacological effects of essential oil from DZHP. MATERIALS AND METHODS: The composition of the essential oil from DZHP was analyzed by gas chromatography/mass spectrometry (GC/MS)...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27794261/differentiation-of-conductive-cells-a-matter-of-life-and-death
#16
REVIEW
Jung-Ok Heo, Bernhard Blob, Ykä Helariutta
Two major conducting tissues in plants, phloem and xylem, are composed of highly specialized cell types adapted to long distance transport. Sieve elements (SEs) in the phloem display a thick cell wall, callose-rich sieve plates and low cytoplasmic density. SE differentiation is driven by selective autolysis combined with enucleation, after which the plasma membrane and some organelles are retained. By contrast, differentiation of xylem tracheary elements (TEs) involves complete clearance of the cellular components by programmed cell death followed by autolysis of the protoplast; this is accompanied by extensive deposition of lignin and cellulose in the cell wall...
February 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/27687541/cholesterol-rich-membrane-microdomains-modulate-wnt-%C3%AE-catenin-morphogen-gradient-during-xenopus-development
#17
Alice H Reis, Marcela M Moreno, Lorena A Maia, Fernanda P Oliveira, Andressa S Santos, José Garcia Abreu
Wnt/β-catenin has been described as crucial for dorsal-ventral and antero-posterior patterning, playing multiple roles at different stages of development. Cholesterol-rich membrane microdomains (CRMMs), cholesterol- and sphingolipid-enriched domains of the plasma membrane, are known as platforms for signaling pathways. Although we have demonstrated the importance of the CRMMs for head development, how they participate in prechordal plate formation and embryo axis patterning remains an open question. Moreover, the participation of the CRMMs in the Wnt/β-catenin signaling pathway activity in vivo is unclear, particularly during embryonic development...
November 2016: Mechanisms of Development
https://www.readbyqxmd.com/read/27583354/histological-response-to-platelet-rich-plasma-added-to-polypropylene-mesh-implemented-in-rabbits
#18
Oscar Rubini Ávila, Natália Gomes Parizzi, Ana Paula Mayumi Souza, Dayane Silvestre Botini, João Ytimura Alves, Silvio Henrique Maia Almeida
INTRODUCTION: The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response. PURPOSE: To investigate the changes caused by PRP associated with the implantation of PPM in the abdomen of female rabbits, in the production of collagen I and III and the inflammatory infiltrate (ININ)...
September 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/27216879/eviscerated-corneas-as-tissue-source-for-ex-vivo-expansion-of-limbal-epithelial-cells-on-platelet-rich-plasma-gels
#19
Leonard G Heydenrych, Donald F du Toit, Colleen M Aldous
Purpose/Aim of the study: To assess if corneal epithelium can be cultured ex vivo from corneas eviscerated due to irretrievable trauma, according to a cell culture method that made use of autologous platelet-rich plasma (A-PRP) as culture substrate. To compare corneal epithelium cultured ex vivo from corneas eviscerated following trauma using A-PRP combined with Dulbecco's modified Eagles medium (DMEM), versus DMEM alone. MATERIALS AND METHODS: This was a laboratory case-controlled study of human corneal cells cultured in a mixture of A-PRP and DMEM, versus DMEM alone from six eviscerated corneas...
December 2016: Current Eye Research
https://www.readbyqxmd.com/read/27148928/the-efficacy-of-platelet-rich-plasma-in-the-treatment-of-rib-fractures
#20
Samil Gunay, Huseyin Candan, Rahsan Yılmaz, Irfan Eser, Umit Aydoğmus
Background Rib fracture is the most common result of thoracic traumas. Intrapulmonary shunt, alveolar capillary membrane damage, intra-alveolar hemorrhage, and hypoxia may develop following rib fractures. Therefore, prompt treatment is important. The aim of this experimental study was to analyze the effects of platelet-rich plasma (PRP) on rib fractures to secure a speedier and more efficient treatment method. Materials and Methods The study involved 18 New Zealand white rabbits, randomly divided into three groups as Group 1, the sham group with no surgical intervention; Group 2, the control group in which simple rib fractures were applied and no treatment; and Group 3, in which rib fractures were applied and then PRP treatment was administered...
October 2017: Thoracic and Cardiovascular Surgeon
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