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https://www.readbyqxmd.com/read/29600207/treating-a-recalcitrant-non-union-of-the-radius-using-autogenous-bone-equine-bone-paste-equine-demineralized-bone-matrix-platelet-rich-plasma-and-bone-marrow-aspirate-a-case-report
#1
Ferdinando Da Rin De Lorenzo
Introduction: Non-unions at forearms are usually challenging and difficult to treat. If additionally, an infection is present, reconstructive surgery should be planned only after full debridement, antibiotic treatment, and confirmation, based on clinical observation and laboratory tests that the infection has subsided. Bone grafting may be required for reconstruction. The use of autogenous bone calls for a second surgical site with an increased risk of morbidity. Using bone substitutes may reduce the need for autogenous bone...
November 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/29393973/application-of-tendon-plating-to-manage-failed-calcaneal-tendon-repairs-in-a-dog
#2
Eric M Zellner, Michael J Hale, Karl H Kraus
OBJECTIVE: To report the application of a plate to treat a complicated calcaneal tendon rupture and the resulting outcome. STUDY DESIGN: Clinical case report. ANIMALS: A 3-year-old Labrador retriever. METHODS: A 3-year-old Labrador retriever was treated for a partially ruptured calcaneal tendon. Two primary tendon repairs with sutures and external fixation had failed. A veterinary cuttable plate was placed to internally span the tendon over the primary repair...
February 2, 2018: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29367906/proliferation-and-differentiation-of-stem-cells-in-contact-with-eluate-from-fibrin-rich-plasma-membrane
#3
Fernanda Gimenez de Souza, Beatriz Luci Fernandes, Carmen Lucia Kuniyoshi Rebelatto, Alessandra Melo de Aguiar, Letícia Fracaro, Paulo Roberto Slud Brofman
Objective: To evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. Method: FRP membranes were obtained by centrifugation of peripheral blood from two healthy donors, cut, and maintained in culture plate wells for 48 h to prepare the fibrin eluate. The SCATh were isolated from adipose tissue by collagenase digestion solution, and expanded in vitro...
January 2018: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/29359696/antimicrobial-effects-of-platelet-rich-plasma-against-selected-oral-and-periodontal-pathogens
#4
Reham L Aggour, Lina Gamil
Antimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients...
March 30, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29299496/effects-of-human-adipose-derived-stem-cells-and-platelet-rich-plasma-on-healing-response-of-canine-alveolar-surgical-bone-defects
#5
Reihaneh Shafieian, Maryam Moghaddam Matin, Amin Rahpeyma, Alireza Fazel, Hamideh Salari Sedigh, Ariane Sadr Nabavi, Halimeh Hassanzadeh, Alireza Ebrahimzadeh-Bideskan
Background: Due to the known disadvantages of autologous bone grafting, tissue engineering approaches have become an attractive method for ridge augmentation in dentistry. To the best of our knowledge, this is the first study conducted to evaluate the potential therapeutic capacity of PRP-assisted hADSCs seeded on HA/TCP granules on regenerative healing response of canine alveolar surgical bone defects. This could offer a great advantage to alternative approaches of bone tissue healing-induced therapies at clinically chair-side procedures...
November 2017: Archives of Bone and Joint Surgery
https://www.readbyqxmd.com/read/29286412/basic-research-in-plasma-medicine-a-throughput-approach-from-liquids-to-cells
#6
Sander Bekeschus, Anke Schmidt, Felix Niessner, Torsten Gerling, Klaus-Dieter Weltmann, Kristian Wende
In plasma medicine, ionized gases with temperatures close to that of vertebrate systems are applied to cells and tissues. Cold plasmas generate reactive species known to redox regulate biological processes in health and disease. Pre-clinical and clinical evidence points to beneficial effects of plasma treatment in the healing of chronic ulcer of the skin. Other emerging topics, such as plasma cancer treatment, are receiving increasing attention. Plasma medical research requires interdisciplinary expertise in physics, chemistry, and biomedicine...
November 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29146168/bone-marrow-derived-mesenchymal-stromal-cells-enhanced-by-platelet-rich-plasma-maintain-adhesion-to-scaffolds-in-arthroscopic-simulation
#7
Alexander R Hoberman, Carl Cirino, Mary Beth McCarthy, Mark P Cote, Leo Pauzenberger, Knut Beitzel, Augustus D Mazzocca, Felix Dyrna
PURPOSE: To assess the response of bone marrow-derived mesenchymal stromal cells (bMSCs) enhanced by platelet-rich plasma (PRP) in the setting of a normal human tendon (NHT), a demineralized bone matrix (DBM), and a fibrin scaffold (FS) with simulated arthroscopic mechanical washout stress. METHODS: Bone marrow was aspirated from the humeral head and concentrated. BMSCs were counted, plated, and grown to confluence. Cells were seeded onto 3 different scaffolds: (1) NHT, (2) DBM, and (3) FS...
November 13, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29023589/light-transmission-aggregometry-using-pre-coated-microtiter-plates-and-a-victor-x5-plate-reader
#8
Pernille Just Vinholt, Mads Nybo, Camilla Brødsgaard Nielsen, Anne-Mette Hvas
BACKGROUND: Light transmission aggregometry (LTA) can be performed with microtiter plates (96-well LTA). When conducting LTA, an agonist is added to platelet-rich plasma and the sample is shaken for minutes after which absorbance readings are done. Platelet aggregation is detected as decrease in absorbance. However, the classical method is cumbersome and therefore microtiter plates can be used for concomitant testing of multiple samples. Furthermore, it would be convenient to prepare the plate in advance of platelet aggregation testing...
2017: PloS One
https://www.readbyqxmd.com/read/28884628/the-effect-of-tannic-acid-on-bone-mechanical-and-geometric-properties-bone-density-and-trabecular-histomorphometry-as-well-as-the-morphology-of-articular-and-growth-cartilages-in-rats-co-exposed-to-cadmium-and-lead-is-dose-dependent
#9
Ewa Tomaszewska, Piotr Dobrowolski, Anna Winiarska-Mieczan, Małgorzata Kwiecień, Siemowit Muszyński, Agnieszka Tomczyk
Cadmium (Cd) and lead (Pb) are toxic elements that accumulate to the largest extent in bones. Rats were used to investigate whether tannic acid (TA; 0.5%, 1.0%, 1.5%. 2.0%, or 2.5%) would have a protective effect on the structure and properties of bones in the case of exposure to Cd and Pb (diet: 7 mg Cd/kg and 50 mg Pb/kg) for 6 weeks. The effects of administration of TA in Cd- and Pb-poisoned rats on bone characteristics and the morphology of articular and growth cartilages were determined. All the rats administered Cd and Pb had an enhanced Cd and Pb concentration in blood plasma and bone and reduced bone Ca content irrespective of the TA administration...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/28827850/platelet-poor-plasma-as-a-supplement-for-fibroblasts-cultured-in-platelet-rich-fibrin
#10
Luiz Alexandre Chisini, Sarah Arangurem Karam, Thaís Gioda Noronha, Letícia Regina Morello Sartori, Alissa Schmidt San Martin, Flávio Fernando Demarco, Marcus Cristian Muniz Conde
The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco's Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)(2) to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x10(4) cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days...
June 2017: Acta Stomatologica Croatica
https://www.readbyqxmd.com/read/28823058/polyurethane-gelatin-nanofibrils-neural-guidance-conduit-containing-platelet-rich-plasma-and-melatonin-for-transplantation-of-schwann-cells
#11
Majid Salehi, Mahdi Naseri-Nosar, Somayeh Ebrahimi-Barough, Mohammdreza Nourani, Arash Khojasteh, Saeed Farzamfar, Korosh Mansouri, Jafar Ai
The current study aimed to enhance the efficacy of peripheral nerve regeneration using a biodegradable porous neural guidance conduit as a carrier to transplant allogeneic Schwann cells (SCs). The conduit was prepared from polyurethane (PU) and gelatin nanofibrils (GNFs) using thermally induced phase separation technique and filled with melatonin (MLT) and platelet-rich plasma (PRP). The prepared conduit had the porosity of 87.17 ± 1.89%, the contact angle of 78.17 ± 5.30° and the ultimate tensile strength and Young's modulus of 5...
August 19, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28667397/delayed-union-of-humeral-shaft-fractures-comparison-of-autograft-with-and-without-platelet-rich-plasma-treatment-a-randomized-single-blinded-clinical-trial
#12
Carlos Acosta-Olivo, Abiel Garza-Borjon, Mario Simental-Mendia, Felix Vilchez-Cavazos, Yadira Tamez-Mata, Victor Peña-Martinez
INTRODUCTION: Despite an adequate orthopedic treatment with functional bracing, some patients develop a delayed union in humeral shaft fractures. The objective of the present study was to determine the bone consolidation time among patients with delayed union of diaphyseal humeral fractures who were managed with locking compression plate (LCP) fixation combined with an iliac crest autograft using platelet-rich plasma (PRP) as a co-adjuvant. MATERIALS AND METHODS: This study was a controlled, randomized, experimental, longitudinal, comparative, prospective, blind clinical trial...
September 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28532001/apatite-grown-in-niobium-by-two-step-plasma-electrolytic-oxidation
#13
Bruno Leandro Pereira, Carlos Maurício Lepienski, Irineu Mazzaro, Neide Kazue Kuromoto
Plasma electrolytic oxidation (PEO) of niobium plates were done electrochemically in two steps with electrolytes containing phosphorous and calcium being observed the formation of crystalline apatite. All samples were submitted to a first step of PEO using an electrolyte containing phosphate ions. The second oxidization step was made using three different electrolytes. Some samples were oxidized by PEO in electrolyte containing calcium, while in other samples it was used two mixtures of phosphoric acid and calcium acetate monohydrate solutions...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28524398/anti-inflammatory-and-analgesic-activities-of-terminalia-muelleri-benth-combretaceae
#14
Nouran M Fahmy, Eman Al-Sayed, Mohamed M Abdel-Daim, Abdel Nasser Singab
Preclinical Research The anti-inflammatory and analgesic activities of a polyphenol-rich fraction (TMEF) obtained from Terminalia muelleri Benth. were measured. The analgesic activity of TMEF was tested using acetic acid-induced writhing and hot plate models in mice. The anti-inflammatory activity was assessed using carrageenan-induced paw edema model by measuring PGE2 , TNF-α, IL-1β, and IL-6 plasma levels as well as the paw thickness. TMEF was tested at doses of 100, 200, and 400 mg/kg p.o. and diclofenac sodium was used as a standard (100 mg/kg) in all experiments...
May 19, 2017: Drug Development Research
https://www.readbyqxmd.com/read/28296142/platelet-rich-plasma-for-androgenetic-alopecia-does-it-work-evidence-from-meta-analysis
#15
REVIEW
Salvatore Giordano, Marco Romeo, Petteri Lankinen
INTRODUCTION: The use of platelet-rich plasma (PRP) has increased among different surgical specialities for the treatment of various conditions. Androgenetic alopecia is a common condition, with severe attendant psychosocial implications. PRP injections for hair restoration have become a popular practice among plastic surgeons. We performed a meta-analysis comparing local injection of platelet-rich plasma versus control to evaluate this issue in order to investigate the effectiveness of PRP local injections for androgenetic alopecia...
September 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28236247/humeral-shaft-non-union-after-intramedullary-nailing
#16
G Campochiaro, P Baudi, M Gialdini, A Corradini, V Duca, M Rebuzzi, F Catani
BACKGROUND: The humerus shaft is one of the sites with the largest probability of developing pseudoarthrosis after fracture. We present the results of nine patients with atrophic pseudoarthrosis of humeral shaft treated with angular stability plate associated with allograft and platelet-rich plasma (PRP), after a first treatment with intramedullary nail to correct the fracture. MATERIAL AND METHODS: From January 2012 to December 2014, nine patients were treated for atrophic pseudoarthrosis (PSA) of humeral shaft treated previously using intramedullary nail; seven humeral diaphysis fractures were located in the middle-proximal third and two in the middle third...
August 2017: Musculoskeletal Surgery
https://www.readbyqxmd.com/read/28190827/use-of-autologous-platelet-rich-plasma-for-a-delayed-consolidation-of-a-tibial-fracture-in-a-young-donkey
#17
Vanessa Faillace, Adolfo Maria Tambella, Margherita Fratini, Emanuele Paggi, Fabrizio Dini, Fulvio Laus
A 9-month-old filly donkey was referred for a comminuted diaphyseal fracture of the right tibia. Surgical osteosynthesis, with multiple lag screws and a neutralization plate, was performed for anatomical reconstruction. Despite a good gait condition, delayed bone consolidation and a bone gap were evident on follow up radiographic evaluations. Due to delayed healing, autologous platelet-rich plasma (PRP) was injected on the surface of the tibia. Increased bone consolidation was evident on radiographs one month after the PRP injection...
March 23, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28042260/effect-of-platelet-rich-plasma-concentrations-on-the-proliferation-of-periodontal-cells-an-in-vitro-study
#18
Sara Tavassoli-Hojjati, Mandana Sattari, Tayebeh Ghasemi, Rahil Ahmadi, Abbas Mashayekhi
OBJECTIVE: The purpose of this study was to evaluate the effect of different concentrations of platelet-rich plasma (PRP) on the proliferation of undifferentiated periodontal ligament (PDL) fibroblasts. MATERIALS AND METHODS: The undifferentiated PDL fibroblasts were obtained from two healthy human premolar teeth and cultured in Dulbecco's modified Eagle's medium. Cell wells were divided into five groups. Experimental groups received 0.1%, 5%, or 50% PRP; the positive and negative control groups were cultured in fetal bovine serum (FBS) 12% and in a medium without FBS 12%, respectively...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/27994625/effect-of-mesenchymal-stem-cells-and-platelet-rich-plasma-on-the-bone-healing-of-ovariectomized-rats
#19
Bo Wei, Chengshuo Huang, Mingyan Zhao, Peng Li, Xiang Gao, Junchao Kong, Yanru Niu, Rui Huang, Juanhua Quan, Jinsong Wei, Jiaqi Chu
We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27989570/the-effect-of-tannic-acid-on-the-bone-tissue-of-adult-male-wistar-rats-exposed-to-cadmium-and-lead
#20
Ewa Tomaszewska, Piotr Dobrowolski, Anna Winiarska-Mieczan, Małgorzata Kwiecień, Agnieszka Tomczyk, Siemowit Muszyński
Toxic elements such as cadmium (Cd) and lead (Pb) accumulate to the largest extent in bones. Rats at the age of 12 weeks were used to check whether tannic acid (TA) at the concentration of 0.5%, 1.0%, 1.5%. 2.0% or 2.5% would have a protective effect on the structure and properties of bones in the case of exposure to Cd and Pb (diet: 7mg Cd/kg and 50mg Pb/kg) for 12 weeks. The effects of administration of TA in Cd- and Pb-poisoned rats on bone mechanical and geometric properties, trabecular histomorphometry as well as the morphology of articular and growth cartilages were determined...
March 2, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
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