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Metabolomic acetate

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https://www.readbyqxmd.com/read/29241669/cytokine-contributions-to-alterations-of-the-volatile-metabolome-induced-by-inflammation
#1
Patrick Millet, Maryanne Opiekun, Talia Martin, Gary K Beauchamp, Bruce A Kimball
Several studies demonstrate that inflammation affects body odor. Volatile signals associated with inflammation induced by pyrogens like LPS are detectable both by conspecifics and chemical analyses. However, little is known about the mechanisms which translate detection of a foreign molecule or pathogen into a unique body odor, or even how unique that odor may be. Here, we utilized C57BL/6J trained mice to identify the odor of LPS-treated conspecifics to investigate potential pathways between LPS-induced inflammation and changes in body odor, as represented by changes in urine odor...
December 11, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29227833/vitamin-a-deficiency-in-mice-alters-host-and-gut-microbial-metabolism-leading-to-altered-energy-homeostasis
#2
Yuan Tian, Robert G Nichols, Jingwei Cai, Andrew D Patterson, Margherita T Cantorna
Vitamin A deficiency (A-) is a worldwide public health problem. To better understand how vitamin A status influences gut microbiota and host metabolism, we systematically analyzed urine, cecum, serum and liver samples from vitamin A sufficient (A+) and deficient (A-) mice using 1H NMR-based metabolomics, quantitative (q)PCR and 16S rRNA gene sequencing coupled with multivariate data analysis. The microbiota in the cecum of A- mice showed compositional as well as functional shifts compared to the microbiota from A+ mice...
November 5, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29226610/nuclear-magnetic-resonance-based-metabolomics-identifies-phenylalanine-as-a-novel-predictor-of-incident-heart-failure-hospitalisation-results-from-prosper-and-finrisk-1997
#3
Christian Delles, Naomi J Rankin, Charles Boachie, Alex McConnachie, Ian Ford, Antti Kangas, Pasi Soininen, Stella Trompet, Simon P Mooijaart, J Wouter Jukema, Faiez Zannad, Mika Ala-Korpela, Veikko Salomaa, Aki S Havulinna, Paul Welsh, Peter Würtz, Naveed Sattar
AIMS: We investigated the association between quantified metabolite, lipid and lipoprotein measures and incident heart failure hospitalisation (HFH) in the elderly, and examined whether circulating metabolic measures improve HFH prediction. METHODS AND RESULTS: Overall, 80 metabolic measures from the PROspective Study of Pravastatin in the Elderly at Risk (PROSPER) trial were measured by proton nuclear magnetic resonance spectroscopy (n = 5341; 182 HFH events during 2...
December 11, 2017: European Journal of Heart Failure
https://www.readbyqxmd.com/read/29205045/colon-ascendens-stent-peritonitis-casp-induces-excessive-inflammation-and-systemic-metabolic-dysfunction-in-a-septic-rat-model
#4
Limin Zhang, Yuan Tian, Jianfen Yang, Jieshou Li, Huiru Tang, Yulan Wang
The colon ascendens stent peritonitis (CASP) surgery creates a leakage of intestinal contents, resulting in polymicrobial sepsis associated with a high risk for postoperative multiple organ failure and death in surgical patients. To evaluate the effects of CASP on multiple organs, we analyzed the systemic metabolic consequences in liver, kidney, lung and heart of rats after CASP by employing a combination of metabolomics, clinical chemistry and biological assays. We found that CASP surgery after 18 h caused significant elevations of lipid, amino acids, acetate, choline, PC and GPC in rat liver together with significant depletion of glucose and glycogen...
December 5, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29199568/lingzhi-or-reishi-medicinal-mushroom-ganoderma-lucidum-agaricomycetes-inhibits-candida-biofilms-a-metabolomic-approach
#5
Anuja Bhardwaj, Payal Gupta, Navin Kumar, Jigni Mishra, Ajai Kumar, Rajput Rakhee, Kshipra Misra
This article presents a comparative gas chromatography (GC)-mass spectrometry (MS)-based metabolomic analysis of mycelia and fruiting bodies of the medicinal mushroom Ganoderma lucidum. Three aqueous extracts-mycelia, fruiting bodies, and a mixture of them-and their sequential fractions (methanolic and ethyl acetate), prepared using an accelerated solvent extractor, were characterized by GC-MS to determine volatile organic compounds and by high-performance thin-layer chromatography to quantify ascorbic acid, a potent antioxidant...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29196291/shotgun-metagenomics-and-volatilome-profile-of-the-microbiota-of-fermented-sausages
#6
Ilario Ferrocino, Alberto Bellio, Manuela Giordano, Guerrino Macori, Angelo Romano, Kalliopi Rantsiou, Lucia Decastelli, Luca Cocolin
Changes in the microbial gene content and abundance can be analyzed to detect shifts in the microbiota composition due to the use of a starter culture in food fermentation process with the consequent shift of key metabolic pathways directly connected with product acceptance. Meat fermentation is a complex process involving microbes that metabolize the main components in meat. The breakdown of carbohydrates, proteins and lipids can lead to the formation of volatile organic compounds (VOCs) that can drastically affect the organoleptic characteristics of the final products...
December 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29195936/1h-nmr-based-metabolomic-fingerprinting-to-determine-metabolite-levels-in-serrano-peppers-capsicum-annum-l-grown-in-two-different-regions
#7
Elvia Becerra-Martínez, Elideth Florentino-Ramos, Nury Pérez-Hernández, L Gerardo Zepeda-Vallejo, Nemesio Villa-Ruano, Manuel Velázquez-Ponce, Felipe García-Mendoza, Angel E Bañuelos-Hernández
Chili pepper (Capsicum annuum) is the most important and emblematic condiment in Mexican food. Serrano pepper is a variety of C. annuum that is traditionally cultivated in Mexico and commercialized in local markets. The aim of this study was to describe the 1H NMR metabolomic profiling of the aqueous phase of serrano peppers harvested from two distinct regions, in the states of Veracruz and Oaxaca, Mexico. According to the current results, aspartate citrate, lactate, leucine and sucrose were found at higher amount in the serrano peppers from Veracruz...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29178373/geographic-impact-evaluation-of-the-quality-of-alismatis-rhizoma-by-untargeted-metabolomics-and-quantitative-assay
#8
Jia Wu, Wenzhi Yang, Huiqin Pan, Shuai Yao, Wanying Wu, Dean Guo
The geographic impact on the quality of Alismatis Rhizoma (derived from the tuber of Alisma orientale), a reputable diuretic traditional Chinese medicine, has seldom been evaluated. Here a metabolomics-driven approach targeting the bioactive protostane triterpenes was developed, by incorporating ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry based untargeted metabolite profiling and multiple reaction monitoring quantitative assay, to probe the triterpene differences between Alismatis Rhizoma samples collected from Sichuan, Fujian, and Jiangxi Provinces...
November 27, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29173237/deficiency-of-essential-dietary-n-3-pufa-disrupts-the-caecal-microbiome-and-metabolome-in-mice
#9
Ruairi C Robertson, Clara Seira Oriach, Kiera Murphy, Gerard M Moloney, John F Cryan, Timothy G Dinan, R P Ross, Catherine Stanton
n-3 PUFA are lipids that play crucial roles in immune-regulation, cardio-protection and neurodevelopment. However, little is known about the role that these essential dietary fats play in modulating caecal microbiota composition and the subsequent production of functional metabolites. To investigate this, female C57BL/6 mice were assigned to one of three diets (control (CON), n-3 supplemented (n3+) or n-3 deficient (n3-)) during gestation, following which their male offspring were continued on the same diets for 12 weeks...
December 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/29161329/comparative-metabolomics-profiling-of-engineered-saccharomyces-cerevisiae-lead-to-a-strategy-that-improving-%C3%AE-carotene-production-by-acetate-supplementation
#10
Xiao Bu, Liang Sun, Fei Shang, Guoliang Yan
A comparative metabolomic analysis was conducted on recombinant Saccharomyces cerevisiae strain producing β-carotene and the parent strain cultivated with glucose as carbon source using gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based approach. The results showed that most of the central intermediates associated with amino acids, carbohydrates, glycolysis and TCA cycle intermediates (acetic acid, glycerol, citric acid, pyruvic acid and succinic acid), fatty acids, ergosterol and energy metabolites were produced in a lower amount in recombinant strain, as compared to the parent strain...
2017: PloS One
https://www.readbyqxmd.com/read/29154733/metabolomic-analysis-of-alterations-in-lipid-oxidation-carbohydrate-and-amino-acid-metabolism-in-dairy-goats-caused-by-exposure-to-aflotoxin-b1
#11
Jianbo Cheng, Shuai Huang, Caiyun Fan, Nan Zheng, Yangdong Zhang, Songli Li, Jiaqi Wang
The purposes of this study were to investigate the systemic and characteristic metabolites in the serum of dairy goats induced by aflatoxin B1 (AFB1) exposure and to further understand the endogenous metabolic alterations induced by it. A nuclear magnetic resonance (NMR)-based metabonomic approach was used to analyse the metabolic alterations in dairy goats that were induced by low doses of AFB1 (50 µg/kg DM). We found that AFB1 exposure caused significant elevations of glucose, citrate, acetate, acetoacetate, betaine, and glycine yet caused reductions of lactate, ketone bodies (acetate, β-hydroxybutyrate), amino acids (citrulline, leucine/isoleucine, valine, creatine) and cell membrane structures (choline, lipoprotein, N-acetyl glycoproteins) in the serum...
November 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/29152916/serum-and-urine-metabolomics-study-reveals-a-distinct-diagnostic-model-for-cancer-cachexia
#12
Quan-Jun Yang, Jiang-Rong Zhao, Juan Hao, Bin Li, Yan Huo, Yong-Long Han, Li-Li Wan, Jie Li, Jinlu Huang, Jin Lu, Gen-Jin Yang, Cheng Guo
BACKGROUND: Cachexia is a multifactorial metabolic syndrome with high morbidity and mortality in patients with advanced cancer. The diagnosis of cancer cachexia depends on objective measures of clinical symptoms and a history of weight loss, which lag behind disease progression and have limited utility for the early diagnosis of cancer cachexia. In this study, we performed a nuclear magnetic resonance-based metabolomics analysis to reveal the metabolic profile of cancer cachexia and establish a diagnostic model...
November 19, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29145079/microbial-transcript-and-metabolome-analysis-uncover-discrepant-metabolic-pathways-in-autotrophic-and-mixotrophic-anammox-consortia
#13
Ying Feng, Yunpeng Zhao, Yongzhao Guo, Sitong Liu
The ability of anammox bacteria to utilize organic matter has drawn extensive attention. However, the metabolic discrepancies between autotrophic and mixotrophic anammox consortia need to be further explored. Here, microbial transcript and metabolomic analysis were conducted for the samples harvested in the reactors and batch assays to investigate the phenotype discrepancies and intrinsic causes in autotrophic and mixotrophic anammox consortia. Results showed that metabolically active community structures did not show significant difference between autotrophic and mixotrophic anammox consortia (C/N = 0...
October 31, 2017: Water Research
https://www.readbyqxmd.com/read/29144590/metabolic-profiling-of-normal-hepatocyte-and-hepatocellular-carcinoma-cells-via-1-h-nuclear-magnetic-resonance-spectroscopy
#14
Yang Chen, Zhong Chen, Jiang-Hua Feng, Yun-Bin Chen, Nai-Shun Liao, Ying Su, Chang-Yan Zou
Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Multivariate and univariate statistical analyses were utilized to identify characteristic metabolites from cells and cultured media. Elevated levels of acetate, creatine, isoleucine, leucine, and phenylalanine were observed in HepG2 cells, suggesting more active in gathering nutrient components along with altered amino acid metabolisms and enhanced lipid metabolism...
November 16, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29141132/intra-species-volatile-interactions-affects-growth-rates-and-exometabolomes-in-aspergillus-oryzae-kccm-60345
#15
Digar Singh, Choong Hwan Lee
The volatile organic compounds (VOCs) are increasingly been recognized as the chemical mediators of mold interactions shaping their community dynamic, growth, and metabolism. Herein, we selectively examined the time-correlated (0 D - 11 D) effects of intra-species VOCs mediated interactions (VMI) on Aspergillus oryzae KCCM 60345 (S1), following co-cultivation with partner strain A. oryzae KACC 44967 (S2), in specially designed twin plate assembly. The comparative evaluation of S1VMI (S1 subjected to VMI with S2) and its control (S1con) showed a notable disparity in their radial growth (S1VMI<S1con) at 5 D, protease activity (S1VMI>S1con) at 3-5 D, amylase activity (S1VMI<S1con) at 3-5 D, and antioxidant levels (S1VMI>S1con) at 3 D...
November 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29136753/-metabolomics-study-of-tris-2-chloroethyl-phosphate-induced-hepaotoxicity-and-nephrotoxicity-in-sprague-dawley-rats
#16
W Q Yang, F Zhao, L Li, Y J Fang
Objective: To discuss the potential toxic target organ and the toxic effects and mechanisms of tris (2-chloroethyl) phosphate (TCEP) on SD rats. Methods: 40 female SD rats weaning from milk for 21 days, weighted (50±2.3)g were selected as subjects and marked by the weight. They were randomly divided into 4 groups, namely control group, 50 (L), 100 (M) and 250 (H) mg·kg(-1)·d(-1) dose of TCEP group. Each group has 10 rats, and administrated the corresponding dose of drug or vehicle by mouth, quaque die for 60 days...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29125557/early-response-monitoring-following-radiation-therapy-by-using-18-f-fdg-and-11-c-acetate-pet-in-prostate-cancer-xenograft-model-with-metabolomics-corroboration
#17
Yi-Hsiu Chung, Cheng-Kun Tsai, Chiun-Chieh Wang, Hsi-Mu Chen, Kuan-Ying Lu, Han Chiu, Yu-Chun Lin, Tzu-Chen Yen, Gigin Lin
We aim to characterize the metabolic changes associated with early response to radiation therapy in a prostate cancer mouse model by 2-deoxy-2-[(18)F]fluoro-d-glucose ([(18)F]FDG) and [(11)C]acetate ([(11)C]ACT) positron emission tomography, with nuclear magnetic resonance (NMR) metabolomics corroboration. [(18)F]FDG and [(11)C]ACT PET were performed before and following irradiation (RT, 15Gy) for transgenic adenocarcinoma of mouse prostate xenografts. The underlying metabolomics alterations of tumor tissues were analyzed by using ex vivo NMR...
November 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29124651/novel-quantitative-insights-into-carbon-sources-for-synthesis-of-poly-hydroxybutyrate-in-synechocystis-pcc-6803
#18
Vaishali Dutt, Shireesh Srivastava
Many freshwater cyanobacteria accumulate polyhydroxybutyrate (PHB) under nitrogen or phosphorus deprivation. While prior literature has shed lights on transcriptomic and metabolomic changes in the model cyanobacterium Synechocystis PCC 6803 cells, the quantitative contributions of the newly fixed carbon following nitrogen deprivation or the externally added acetate to PHB synthesis are not clear. Similarly, it is not clear how photomixotrophy affects precursor contributions. In this study, we show that (i) the pre-growth mode (photoautotrophic or photomixotrophic), while significantly impacting glycogen levels, does not have any significant effect on PHB levels, (ii) the carbon fixed following nitrogen deprivation contributes 26% of C for PHB synthesis in photoautotrophically pre-grown cells and its contribution to the PHB synthesis goes down with the addition of acetate at the resuspension phase or with photomixotrophic pre-growth, (iii) the acetate added at the start of nitrogen deprivation, doubles the intracellular PHB levels and contributes 44-48% to PHB synthesis and this value is not greatly affected by how the cells were pre-grown...
November 9, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/29113318/follicular-metabolic-changes-and-effects-on-oocyte-quality-in-polycystic-ovary-syndrome-patients
#19
Yan Zhang, Lingyan Liu, Tai-Lang Yin, Jing Yang, Cheng-Liang Xiong
Polycystic ovary syndrome (PCOS) is a common complex and heterogeneous disorder, affecting up to 10% women at reproductive age. It causes three fourth of the ovulatory infertility and PCOS patients often give poor IVF quality. Although some metabolic profiles have been investigated in PCOS patient sera and urine, the follicular fluid, providing fruitful biochemical information about oocyte environment during development has been ignored. In this work, based on NMR metabolomics approach, metabolic profile of follicular fluid of PCOS patients has been explored and compared with healthy controls...
October 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/29105990/improving-negative-esi-lc-ms-lipidomic-analysis-of-human-plasma-using-acetic-acid-as-a-mobile-phase-additive
#20
Cian Monnin, Parsram Ramrup, Carolann Daigle-Young, Dajana Vuckovic
RATIONALE: Mobile phase additives in LC-MS are used to improve peak shape, analyte ionization efficiency and method coverage. Both basic and acidic mobile phases have been used successfully for negative ESI, but very few systematic investigations exist to date to justify the choice of mobile phase. Acetic acid was previously shown to improve ionization in untargeted metabolomics of urine, but has not been investigated in lipidomics. The goal of this study was to systematically compare the performance of acetic acid to other commonly employed additives in negative ESI-LC-MS lipidomics...
November 6, 2017: Rapid Communications in Mass Spectrometry: RCM
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