keyword
MENU ▼
Read by QxMD icon Read
search

Metabolomic acetate

keyword
https://www.readbyqxmd.com/read/29032784/spent-culture-medium-analysis-from-individually-cultured-bovine-embryos-demonstrates-metabolomic-differences
#1
Kayla J Perkel, Pavneesh Madan
Spent culture medium can provide valuable information regarding the physiological state of a bovine preimplantation embryos through non-invasive analysis of the sum/depleted metabolite constituents. Metabolomics has become of great interest as an adjunct technique to morphological and cleavage-rate assessment, but more importantly, in improving our understanding of metabolism. In this study, in vitro produced bovine embryos developing at different rates were evaluated using proton nuclear magnetic resonance (1H NMR)...
October 16, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28934947/-1-h-nmr-spectroscopy-based-metabolomics-analysis-for-the-diagnosis-of-symptomatic-e-coli-associated-urinary-tract-infection-uti
#2
Milena Lussu, Tania Camboni, Cristina Piras, Corrado Serra, Francesco Del Carratore, Julian Griffin, Luigi Atzori, Aldo Manzin
BACKGROUND: Urinary tract infection (UTI) is one of the most common diagnoses in girls and women, and to a lesser extent in boys and men younger than 50 years. Escherichia coli, followed by Klebsiella spp. and Proteus spp., cause 75-90% of all infections. Infection of the urinary tract is identified by growth of a significant number of a single species in the urine, in the presence of symptoms. Urinary culture is an accurate diagnostic method but takes several hours or days to be carried out...
September 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28929691/-deuterated-surrogate-and-uplc-ms-ms-method-for-simultaneous-determination-of-22-endocannabinoids-in-ovariectomized-rat-plasma
#3
Shi-Xie Xiang, Ying Xu, Jing-Jing Zhu, Zhi-Min Wang, Dong Zhang, Liang-Mian Chen, Wei-Hong Feng
A new method based on ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 22 endocannabinoids(eCBs) and relevant compounds in ovariectomized rat plasma. After being extracted by solid-phase column(SPE), the plasma samples were detected by using UPLC-MS/MS. Analysis was carried out with ACQUITY UPLC BEH C₁₈ column. The mobile phase was 0.1% acetic acid solution(A)-acetonitrile and isopropanol(9∶1, B) for the gradient elution. In the positive ion multiple reaction monitoring(MRM) mode, deuterated reagents were taken as standard alternatives to calculate recoveries and simultaneously quantify 22 endocannabinoids...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28928712/metabolomics-as-a-tool-for-the-characterization-of-drug-resistant-epilepsy
#4
Federica Murgia, Antonella Muroni, Monica Puligheddu, Lorenzo Polizzi, Luigi Barberini, Gianni Orofino, Paolo Solla, Simone Poddighe, Francesco Del Carratore, Julian L Griffin, Luigi Atzori, Francesco Marrosu
PURPOSE: Drug resistance is a critical issue in the treatment of epilepsy, contributing to clinical emergencies and increasing both serious social and economic burdens on the health system. The wide variety of potential drug combinations followed by often failed consecutive attempts to match drugs to an individual patient may mean that this treatment stage may last for years with suboptimal benefit to the patient. Given these challenges, it is valuable to explore the availability of new methodologies able to shorten the period of determining a rationale pharmacologic treatment...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28916042/urinary-intermediates-of-tryptophan-as-indicators-of-the-gut-microbial-metabolism
#5
Tereza Pavlova, Veronika Vidova, Julie Bienertova-Vasku, Petr Janku, Martina Almasi, Jana Klanova, Zdenek Spacil
While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM)...
September 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28881984/estimation-of-time-varying-growth-uptake-and-excretion-rates-from-dynamic-metabolomics-data
#6
Eugenio Cinquemani, Valérie Laroute, Muriel Cocaign-Bousquet, Hidde de Jong, Delphine Ropers
Motivation: Technological advances in metabolomics have made it possible to monitor the concentration of extracellular metabolites over time. From these data, it is possible to compute the rates of uptake and excretion of the metabolites by a growing cell population, providing precious information on the functioning of intracellular metabolism. The computation of the rate of these exchange reactions, however, is difficult to achieve in practice for a number of reasons, notably noisy measurements, correlations between the concentration profiles of the different extracellular metabolites, and discontinuties in the profiles due to sudden changes in metabolic regime...
July 15, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28869288/exploring-the-mechanism-of-jieduquyuziyin-prescription-on-systemic-lupus-erythematosus-by-gc-ms-based-urine-metabolomics
#7
Jing Wei, Jun Gao, Xinghong Ding
A urine metabolomics method based on gas chromatography mass spectrometry (GC-MS) was developed in order to investigate the metabolites characters of systemic lupus erythematosus (SLE) and therapeutic effects of jieduquyuziyin prescription (JP). The urinary metabolic profiles in urine specimens of the SLE model mice (MRL/lpr) group, prednisone acetate (PA)-treated SLE mice group, JP-treated SLE mice group, and control group (C57BL/6 J) after the administration were analyzed by GC-MS. These metabolic profiles were then processed by multivariate analysis, in particular Mass Profiler Professional (MPP), SIMCA-P and partial least-squares discriminant analysis (PLS-DA)...
September 4, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28865386/response-of-rhizobium-to-cd-exposure-a-volatile-perspective
#8
Paulo Cardoso, Magda Santos, Rosa Freitas, Sílvia M Rocha, Etelvina Figueira
The volatile metabolome of Rhizobium sp. strain E20-8 exposed to three concentrations of cadmium (2.5, 5.0 and 7.5 μM) was screened using comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GC × GC-ToFMS), combined with headspace solid phase microextraction (HS-SPME). Cd exposure induced a global increase in the concentration of volatile organic compounds (VOCs) both intra and extracellularly. Peak areas of several linear alkanes, ketones, aldehydes, alcohols, terpenic and volatile sulfur compounds, and one ester (ethyl acetate), were especially increased when compared with the control condition (no Cd)...
August 30, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28847040/metabolic-effects-of-hedyotis-diffusa-on-rats-bearing-walker-256-tumor-revealed-by-nmr-based-metabolomics
#9
Zhiyong Wang, Kuo Gao, Can Xu, Jian Gao, Yujing Yan, Yingfeng Wang, Zhongfeng Li, Jianxin Chen
Hedyotis diffusa, a traditional Chinese herbal medicine, is widely used for oncotherapy and shows a positive effect in the clinical treatment. But its mechanism of anticancer activities is complicated and unclear. This study was undertaken to assess the therapeutic effects and reveal detailed mechanisms of H. diffusa for oncotherapy. A Walker 256 tumor-bearing rat model was established, and metabolomic profiles of plasma and urine were obtained from (1) H NMR technique. Multivariate statistical analysis methods were used to characterize the discriminating metabolites between control (C), Walker 256 tumor-bearing rats model (M), and H...
August 28, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28844081/comparison-of-global-metabolite-extraction-strategies-for-soybeans-using-uhplc-hrms
#10
Iqbal Mahmud, Sandi Sternberg, Michael Williams, Timothy J Garrett
Metabolism, downstream effectors of genomics, transcriptomics, and proteomics, can determine the potential of phenotype of an organism including plants. Profiling the global scenario of metabolism requires optimization of different solvent extraction methods. Here, we report an approach comparing three different metabolite extraction strategies, including ammonium acetate/methanol (AAM), water/methanol (WM), and sodium phosphate/methanol (PM) in soybean plant using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS)...
August 26, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28843023/physiological-and-metabolomic-analysis-of-issatchenkia-orientalis-mty1-with-multiple-tolerance-for-cellulosic-bioethanol-production
#11
Yeong-Je Seong, Hye-Jin Lee, Jung-Eun Lee, Sooah Kim, Do Yup Lee, Kyoung Heon Kim, Yong-Cheol Park
Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 was isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions was characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100 g/L glucose, I. orientalis MTY1 possessed wider growth ranges at pH 2-8 and 30-45 oC than a conventional yeast of Saccharomyces cerevisiae D452-2...
August 26, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28836978/diurnal-variation-of-nmr-based-blood-metabolites-in-calves-fed-a-high-plane-of-milk-replacer-a-pilot-study
#12
Morteza H Ghaffari, Jayden A R MacPherson, Harma Berends, Michael A Steele
BACKGROUND: Blood profiles have been used to monitor herd health status, diagnose disorders, and predict the risk of diseases in cattle and calves. Characterizing plasma metabolites in dairy calves could provide further insight into daily metabolic variations and the mechanisms that lead to metabolic diseases. In addition, by understanding physiological ranges of plasma metabolites relative to meal and the time of feeding in healthy animals, veterinarians can accurately diagnose abnormalities with a blood test...
August 23, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28804274/non-invasive-urinary-metabolomic-profiling-discriminates-prostate-cancer-from-benign-prostatic-hyperplasia
#13
Clara Pérez-Rambla, Leonor Puchades-Carrasco, María García-Flores, José Rubio-Briones, José Antonio López-Guerrero, Antonio Pineda-Lucena
INTRODUCTION: Prostate cancer (PCa) is one of the most common malignancies in men worldwide. Serum prostate specific antigen (PSA) level has been extensively used as a biomarker to detect PCa. However, PSA is not cancer-specific and various non-malignant conditions, including benign prostatic hyperplasia (BPH), can cause a rise in PSA blood levels, thus leading to many false positive results. OBJECTIVES: In this study, we evaluated the potential of urinary metabolomic profiling for discriminating PCa from BPH...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28759594/rapid-discrimination-of-strain-dependent-fermentation-characteristics-among-lactobacillus-strains-by-nmr-based-metabolomics-of-fermented-vegetable-juice
#14
Satoru Tomita, Katsuichi Saito, Toshihide Nakamura, Yasuyo Sekiyama, Jun Kikuchi
In this study, we investigated the applicability of NMR-based metabolomics to discriminate strain-dependent fermentation characteristics of lactic acid bacteria (LAB), which are important microorganisms for fermented food production. To evaluate the discrimination capability, six type strains of Lactobacillus species and six additional L. brevis strains were used focusing on i) the difference between homo- and hetero-lactic fermentative species and ii) strain-dependent characteristics within L. brevis. Based on the differences in the metabolite profiles of fermented vegetable juices, non-targeted principal component analysis (PCA) clearly separated the samples into those inoculated with homo- and hetero-lactic fermentative species...
2017: PloS One
https://www.readbyqxmd.com/read/28750646/metabolomics-of-rhabdomyosarcoma-cell-during-echovirus-30-infection
#15
Sarika Tiwari, Tapan N Dhole
BACKGROUND: Echovirus 30 (E30) causes acute aseptic meningitis. Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. The effect of viral infection within a host cell metabolic activity remains unclear. METHODS: To gain an insight into cell-virus interaction during E30 infection we used a human rhabdomyosarcoma cell line. In a new approach to metabolomics, (1H) NMR was used to measure the level of various cellular metabolites at different times of infection and morphological examination of the cells...
July 27, 2017: Virology Journal
https://www.readbyqxmd.com/read/28746356/metabolomic-profiling-of-csf-in-multiple-sclerosis-and-neuromyelitis-optica-spectrum-disorder-by-nuclear-magnetic-resonance
#16
Hyun-Hwi Kim, In Hye Jeong, Ja-Shil Hyun, Byung Soo Kong, Ho Jin Kim, Sung Jean Park
Multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) are inflammatory diseases of the central nervous system. Although several studies have characterized the metabolome in the cerebrospinal fluid (CSF) from MS and NMOSD patients, comparative analyses between them and between the relapse and the remission of each disease have not been performed. Both univariate and multivariate analyses were used to compare 1H-NMR spectra of CSF from MS, NMOSD, and healthy controls (HCs). The statistical analysis showed alterations of eight metabolites that were dependent on the disease...
2017: PloS One
https://www.readbyqxmd.com/read/28734161/evaluation-of-the-effect-of-extraction-solvent-and-organ-selection-on-the-chemical-profile-of-astragalus-spinosus-using-hptlc-multivariate-image-analysis
#17
Eman Shawky, Dina A Selim
The evaluation of extraction protocols for untargeted and targeted metabolomics was implemented for root and aerial organs of Astragalus spinosus in this work. The efficiency and complementarity of commonly used extraction solvents, namely petroleum ether, methylene chloride, ethyl acetate and n-butanol were considered for method evaluation using chemometric techniques in conjunction with new, simple, and fast high performance thin layer chromatography (HPTLC) method for fingerprint analysis by extracting information from a digitalized HPTLC plate using ImageJ software...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28725238/a-non-targeted-metabolomics-approach-unravels-the-vocs-associated-with-the-tomato-immune-response-against-pseudomonas-syringae
#18
María Pilar López-Gresa, Purificación Lisón, Laura Campos, Ismael Rodrigo, José Luis Rambla, Antonio Granell, Vicente Conejero, José María Bellés
Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These compounds may perform direct defensive functions, i.e., acting as antioxidant, antibacterial, or antifungal agents, or indirectly by signaling the activation of the plant's defensive responses. Using a non-targeted GC-MS metabolomics approach, we identified the profile of the VOCs associated with the differential immune response of the Rio Grande tomato leaves infected with either virulent or avirulent strains of Pseudomonas syringae DC3000 pv...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28724254/effects-of-calcium-on-the-performance-bacterial-population-and-microbial-metabolism-of-a-denitrifying-phosphorus-removal-system
#19
Hongliang Dai, Yifeng Wu, Lihong Peng, Zheqin Dai, Xiang Li, Xiwu Lu
A sequencing batch reactor was operated to study the effects of influent Ca(2+) on the efficiency, bacterial population, and microbial metabolism of denitrifying phosphorus removal system. Results showed that high Ca(2+) loading (≥80mg/L) significantly inhibited the performance of simultaneous nitrogen and phosphorus removal. The abundance of phosphorus removal-related organisms (Dechloromonas and Candidatus Accumulibacter) decreased with increasing Ca(2+) concentration from 20 to 140mg/L, while the abundance of glycogen-accumulating organisms and other bacteria increased...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28722670/follicular-metabolic-changes-and-effects-on-oocyte-quality-in-polycystic-ovary-syndrome-patients
#20
Yan Zhang, Lingyan Liu, Tai-Lang Yin, Jing Yang, Cheng-Liang Xiong
Polycystic ovary syndrome (PCOS) is a common complex and heterogeneous disorder, affecting up to 10% women at reproductive age. It causes three fourth of the ovulatory infertility and PCOS patients often give poor IVF quality. Although some metabolic profiles have been investigated in PCOS patient sera and urine, the follicular fluid, providing fruitful biochemical information about oocyte environment during development has been ignored. In this work, based on NMR metabolomics approach, metabolic profile of follicular fluid of PCOS patients has been explored and compared with healthy controls...
July 6, 2017: Oncotarget
keyword
keyword
120033
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"