Read by QxMD icon Read

Metabolomic acetate

Anupam Guleria, Avadhesh Pratap, Durgesh Dubey, Atul Rawat, Smriti Chaurasia, Edavalath Sukesh, Sanat Phatak, Sajal Ajmani, Umesh Kumar, Chunni Lal Khetrapal, Paul Bacon, Ramnath Misra, Dinesh Kumar
Management of patient with Lupus Nephritis (LN) continues to remain a challenge for the treating physicians because of considerable morbidity and even mortality. The search of biomarkers in serum and urine is a focus of researchers to unravel new targets for therapy. In the present study, the utility of NMR-based serum metabolomics has been evaluated for the first time in discriminating LN patients from non-nephritis lupus patients (SLE) and further to get new insights into the underlying disease processes for better clinical management...
October 14, 2016: Scientific Reports
Louise M A Jakobsen, Christian C Yde, Thomas Van Hecke, Randi Jessen, Jette F Young, Stefaan De Smet, Hanne Christine Bertram
SCOPE: The scope of the present study was to investigate the effects of red versus white meat intake on the metabolome of rats. METHODS AND RESULTS: Twenty-four male Sprague-Dawley rats were randomly assigned to 15 days of ad libitum feeding of one of four experimental diets: 1) lean chicken, 2) chicken with lard, 3) lean beef, and 4) beef with lard. Urine, feces, plasma and colon tissue samples were analyzed using (1) H NMR-based metabolomics and real-time PCR was performed on colon tissue to examine the expression of specific genes...
October 13, 2016: Molecular Nutrition & Food Research
Yunfei Yuan, Yupeng Zhao, Jiali Yang, Yueming Jiang, Fei Lu, Yongxia Jia, Bao Yang
Banana is a tropical fruit widely accepted by people over the world. Its chemical composition is critical for its organoleptic properties and nutritional value. In this work, the metabolite changes during postharvest senescence were investigated using NMR spectroscopy. The 1D and 2D NMR spectroscopic information revealed the primary and secondary metabolites in banana fruit, including organic acids, amino acids, carbohydrates and phenolics. Bananas at five senescence stages showed similar chemical profiles, but the levels of the individual compounds varied to a large extent...
March 1, 2017: Food Chemistry
Dahye Yoon, Siwon Kim, Minji Lee, Changshin Yoon, Suhkmann Kim
A (1)H-nuclear magnetic resonance (NMR) spectroscopy with multivariate analysis was applied to detect the toxicity of antiacetylcholinesterase insecticides, methomyl (methyl (1E)-N-(methylcarbamoyloxy)ethanimidothioate) and methidathion (3-(dimethoxyphosphinothioyl sulfanylmethyl)-5-methoxy-1,3,4-thiadiazol-2-one), using zebrafish (Danio rerio) and Chinese bleak (Aphyocypris chinensis). Generally, methomyl and methidathion have been believed not to highly accumulate in fish tissues. However, these pesticides showed their toxicity by altering patterns of whole-body metabolites in neurotransmitter balance, energy metabolism, oxidative stress, and muscle maintenance in low concentrations...
August 12, 2016: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
N K Singhal, H Huang, S Li, R Clements, J Gadd, A Daniels, E E Kooijman, P Bannerman, T Burns, F Guo, D Pleasure, E Freeman, L Shriver, J McDonough
The neuronal mitochondrial metabolite N-acetylaspartate (NAA) is decreased in the multiple sclerosis (MS) brain. NAA is synthesized in neurons by the enzyme N-acetyltransferase-8-like (NAT8L) and broken down in oligodendrocytes by aspartoacylase (ASPA) into acetate and aspartate. We have hypothesized that NAA links the metabolism of axons with oligodendrocytes to support myelination. To test this hypothesis, we performed lipidomic analyses using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-performance thin-layer chromatography (HPTLC) to identify changes in myelin lipid composition in postmortem MS brains and in NAT8L knockout (NAT8L(-/-)) mice which do not synthesize NAA...
October 5, 2016: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
Shuang Zhao, Xian Luo, Liang Li
A key step in metabolomics is to perform accurate relative quantification of the metabolomes in comparative samples with high coverage. Hydroxyl-containing metabolites are an important class of the metabolome with diverse structures and physical/chemical properties. However, many of them are difficult to detect with high sensitivity. We present a high-performance chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) technique for in-depth profiling of the hydroxyl submetabolome. It involves the use of acidic liquid-liquid extraction (LLE) to enrich hydroxyl metabolites into ethyl acetate from an aqueous sample...
October 3, 2016: Analytical Chemistry
Cristina Airoldi, Carlotta Ciaramelli, Marco Fumagalli, Rita Bussei, Valeria Mazzoni, Simona Viglio, Paolo Iadarola, Jan Stolk
The metabolomic analysis of exhaled breath condensate (EBC) may provide insights on both the pathology of pulmonary disorders and the response to therapy. This pilot study describes the ability of nuclear magnetic resonance (NMR)-based metabolomics to discriminate α1-antitrypsin deficient (AATD)-patients, who were diagnosed with moderate to severe emphysema, from healthy individuals. Comparative analysis of samples from these two homogeneous cohorts of individuals resulted in the generation of NMR profiles that were different from both a qualitative and a quantitative point-of-view...
October 5, 2016: Journal of Proteome Research
Gang Shao, Michael MacNeil, Yuanyuan Yao, Roger W Giese
RATIONALE: A method was needed to accomplish solid phase extraction of a large urine volume in a convenient way where resources are limited, towards a goal of metabolome and xenobiotic exposome analysis at another, distant location. METHODS: A porous extraction paddle (PEP) was set up, comprising a porous nylon bag containing extraction particles that is flattened and immobilized between two stainless steel meshes. Stirring the PEP after attachment to a shaft of a motor mounted on the lid of the jar containing the urine accomplishes extraction...
September 14, 2016: Rapid Communications in Mass Spectrometry: RCM
Ghulam Mustafa Kamal, Bin Yuan, Abdullah Ijaz Hussain, Jie Wang, Bin Jiang, Xu Zhang, Maili Liu
It has been a strong consumer interest to choose high quality food products with clear information about their origin and composition. In the present study, a total of 22 Asian soy sauce samples have been analyzed in terms of (13)C-NMR spectroscopy. Spectral data were analyzed by multivariate statistical methods in order to find out the important metabolites causing the discrimination among typical soy sauces from different Asian regions. It was found that significantly higher concentrations of glutamate in Chinese red cooking (CR) soy sauce may be the result of the manual addition of monosodium glutamate (MSG) in the final soy sauce product...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Guangmang Liu, Xianjian Wu, Gang Jia, Xiaoling Chen, Hua Zhao, Jing Wang, Caimei Wu, Jingyi Cai
Arginine regulates growth performance, nutrient metabolism and health effects, but the underlying mechanism remains unknown. This study aims to investigate the effect of dietary arginine supplementation on rat growth performance and urinary metabolome through ¹H-NMR spectroscopy. Twenty rats were randomly assigned to two groups supplemented with 0% or 1.0% l-arginine for 4 weeks. Urine samples were analyzed through NMR-based metabolomics. Arginine supplementation significantly increased the urine levels of 4-aminohippurate, acetate, creatine, creatinine, ethanolamine, formate, hippurate, homogentisate, indoxyl sulfate, and phenylacetyglycine...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
D Arul Pon Daniel, K Thangavel
Breathomics is the metabolomics study of exhaled air. It is a powerful emerging metabolomics research field that mainly focuses on health-related volatile organic compounds (VOCs). Since the quantity of these compounds varies with health status, breathomics assures to deliver noninvasive diagnostic tools. Thus, the main aim of breathomics is to discover patterns of VOCs related to abnormal metabolic processes occurring in the human body. Classification systems, however, are not designed for cost-sensitive classification domains...
July 2016: Journal of Medical Signals and Sensors
Guangmang Liu, Wei Cao, Tingting Fang, Gang Jia, Hua Zhao, Xiaoling Chen, Caimei Wu, Jing Wang
Glutamine and N-carbamylglutamate can enhance growth performance and health in animals, but the underlying mechanisms are not yet elucidated. This study aimed to investigate the effect of glutamine and N-carbamylglutamate supplementation in rat metabolism. Thirty rats were fed a control, glutamine, or N-carbamylglutamate diet for four weeks. Urine samples were analyzed by nuclear magnetic resonance (NMR)-based metabolomics, specifically high-resolution ¹H NMR metabolic profiling combined with multivariate data analysis...
2016: Nutrients
Tomohiro Shimada, Kan Tanaka
UNLABELLED: Regulation of central carbon metabolism has long been an important research subject in every organism. While the dynamics of metabolic flows during changes in available carbon sources have been estimated based on changes in metabolism-related gene expression, as well as on changes in the metabolome, the flux change itself has scarcely been measured because of technical difficulty, which has made conclusions elusive in many cases. Here, we used a monitoring system employing Vibrio fischeri luciferase to probe the intracellular metabolic condition in Escherichia coli Using a batch culture provided with a limited amount of glucose, we performed a time course analysis, where the predominant carbon source shifts from glucose to acetate, and identified a series of sequential peaks in the luciferase activity (peaks 1 to 4)...
October 1, 2016: Applied and Environmental Microbiology
Xinfeng Liu, Jian Gao, Jianxin Chen, Zhiyong Wang, Qi Shi, Hongxue Man, Shuzhen Guo, Yingfeng Wang, Zhongfeng Li, Wei Wang
Type 2 diabetic coronary heart disease (T2DM-CHD) is a kind of serious and complex disease. Great attention has been paid to exploring its mechanism; however, the detailed understanding of T2DM-CHD is still limited. Plasma samples from 15 healthy controls, 13 coronary heart disease (CHD) patients, 15 type 2 diabetes mellitus (T2DM) patients and 28 T2DM-CHD patients were analyzed in this research. The potential biomarkers of CHD and T2DM were detected and screened out by (1)H NMR-based plasma metabolic profiling and multivariate data analysis...
2016: Scientific Reports
Luigi Lucini, Daniela Borgognone, Youssef Rouphael, Mariateresa Cardarelli, Jamila Bernardi, Giuseppe Colla
There is a growing interest among consumers and researchers in the globe artichoke [Cynara cardunculus L. subsp. scolymus (L.) Hegi] leaf extract due to its nutraceutical and therapeutic properties. The application of an abiotic stress such as salinity can activate the stress-signaling pathways, thus enhancing the content of valuable phytochemicals. The aim of this study was to assess the metabolic changes in artichokes by probing the leaf metabolome of artichoke plants grown in a floating system and exposed to a relatively mild (30 mM) potassium chloride (KCl) salt stress...
2016: Frontiers in Plant Science
Jasmine Sati, Biraja Prasad Mohanty, Mohan Lal Garg, Ashwani Koul
Silibinin, a major bioactive flavonolignan in Silybum marianum, has received considerable attention in view of its anticarcinogenic activity. The present study examines its anticancer potential against 7, 12-dimethylbenz(a)anthracene (DMBA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) induced skin cancer. Male LACA mice were randomly segregated into 4 groups: Control, DMBA/TPA, Silibinin and Silibinin+DMBA/TPA. Tumors in DMBA/TPA and Silibinin+DMBA/TPA groups were histologically graded as squamous cell carcinoma...
2016: PloS One
Noam Auslander, Keren Yizhak, Adam Weinstock, Anuradha Budhu, Wei Tang, Xin Wei Wang, Stefan Ambs, Eytan Ruppin
Disrupted regulation of cellular processes is considered one of the hallmarks of cancer. We analyze metabolomic and transcriptomic profiles jointly collected from breast cancer and hepatocellular carcinoma patients to explore the associations between the expression of metabolic enzymes and the levels of the metabolites participating in the reactions they catalyze. Surprisingly, both breast cancer and hepatocellular tumors exhibit an increase in their gene-metabolites associations compared to noncancerous adjacent tissues...
2016: Scientific Reports
B Fatemeh Nobakht M Gh, Afsaneh Arefi Oskouie, Mostafa Rezaei-Tavirani, Rasoul Aliannejad, Salman Taheri, Fariba Fathi, Mohammad Taghi Naseri
Sulfur mustard (SM) is a vesication chemical warfare agent for which there is currently no antidote. Despite years of research, there is no common consensus about the pathophysiological basis of chronic pulmonary disease caused by this chemical warfare agent. In this study, we combined chemometric techniques with nuclear magnetic resonance (NMR) spectroscopy to explore the metabolic profile of sera from SM-exposed patients. A total of 29 serum samples obtained from 17 SM-injured patients, and 12 healthy controls were analyzed by Random Forest...
June 20, 2016: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Livia Roberta Piedade de Oliveira, Carla Martins, Tatiana Kelly Silva Fidalgo, Liana Bastos Freitas-Fernandes, Rafaela de Oliveira Torres, Aline Laignier Soares, Fabio C L Almeida, Ana Paula Valente, Ivete Pomarico Ribeiro de Souza
Metabolomics is an important tool for the evaluation of the human condition, in both health or disease. This study analyzed the salivary components of type I diabetic children (DM1) under six years of age, to assess oral health related to diabetes control, as well as metabolite profiling using NMR. Partial least squared discriminant analysis (PLS-DA) was used to compare healthy (HG) and uncontrolled DM1 subjects that demonstrated a separation between the groups with classificatory performance of ACC = 0.80, R(2) = 0...
August 5, 2016: Journal of Proteome Research
Chuchu Xu, Cheng Xia, Yuhang Sun, Xinhuan Xiao, Gang Wang, Ziling Fan, Shi Shu, Hongyou Zhang, Chuang Xu, Wei Yang
To understand the differences in metabolic changes between cows with ovarian inactivity and estrus cows, we selected cows at 60-90 days postpartum from an intensive dairy farm. According to clinical manifestations, B-ultrasound scan, rectal examination, 10 cows were assigned to the estrus group (A) and 10 to the ovarian inactivity group (B). All plasma samples were analyzed by (1)H-nuclear magnetic resonance spectroscopy to compare plasma metabolomic profiles between the groups. We used multivariate pattern recognition to screen for different metabolites in plasma of anestrus cows...
October 1, 2016: Theriogenology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"