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Metabolomic acetate

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https://www.readbyqxmd.com/read/28442386/arginine-deiminase-pathway-provides-atp-and-boosts-growth-of-the-gas-fermenting-acetogen-clostridium-autoethanogenum
#1
Kaspar Valgepea, Kim Q Loi, James B Behrendorff, Renato de S P Lemgruber, Manuel Plan, Mark P Hodson, Michael Köpke, Lars K Nielsen, Esteban Marcellin
Acetogens are attractive organisms for the production of chemicals and fuels from inexpensive and non-food feedstocks such as syngas (CO, CO2 and H2). Expanding their product spectrum beyond native compounds is dictated by energetics, particularly ATP availability. Acetogens have evolved sophisticated strategies to conserve energy from reduction potential differences between major redox couples, however, this coupling is sensitive to small changes in thermodynamic equilibria. To accelerate the development of strains for energy-intensive products from gases, we used a genome-scale metabolic model (GEM) to explore alternative ATP-generating pathways in the gas-fermenting acetogen Clostridium autoethanogenum...
April 22, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28421296/metabolite-signatures-of-doxorubicin-induced-toxicity-in-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#2
Umesh Chaudhari, James K Ellis, Vilas Wagh, Harshal Nemade, Jürgen Hescheler, Hector C Keun, Agapios Sachinidis
Drug-induced off-target cardiotoxicity, particularly following anti-cancer therapy, is a major concern in new drug discovery and development. To ensure patient safety and efficient pharmaceutical drug development, there is an urgent need to develop more predictive cell model systems and distinct toxicity signatures. In this study, we applied our previously proposed repeated exposure toxicity methodology and performed (1)H NMR spectroscopy-based extracellular metabolic profiling in culture medium of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) exposed to doxorubicin (DOX), an anti-cancer agent...
April 18, 2017: Amino Acids
https://www.readbyqxmd.com/read/28415762/metabonomics-studies-on-serum-and-urine-of-patients-with-breast-cancer-using-1h-nmr-spectroscopy
#3
Jianping Zhou, Yanxia Wang, Xinli Zhang
The aim of this study was to describe a metabolomic study of breast cancer using 1H-NMR combined with bioinformatics analysis. 1H-NMR spectroscopy combined with multi-variate pattern recognition analysis was used to cluster the groups (serum and urine samples from breast cancer patients and healthy controls) and establish a breast-cancer-specific metabolites phenotype. Orthogonalpartial least-squares discriminant analysis (OPLS-DA) was capable of distinguishing serum and urine samples from breast cancer patients and healthy controls and establishing a breast-cancer-specific metabolite profile...
March 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28411229/a-comprehensive-analysis-of-myocardial-substrate-preference-emphasizes-the-need-for-a-synchronized-fluxomic-metabolomic-research-design
#4
Mukundan Ragavan, Alexander Kirpich, Xiaorong Fu, Shawn C Burgess, Lauren M McIntyre, Matthew E Merritt
The heart oxidizes fatty acids, carbohydrates and ketone bodies inside the citric acid cycle (CAC) to generate the reducing equivalents needed for ATP production. Competition between these substrates makes it difficult to estimate the extent of pyruvate oxidation. Previously, hyperpolarized pyruvate detected propionate mediated activation of carbohydrate oxidation, even in the presence of acetate. In this report, the optimal concentration of propionate for activation of glucose oxidation is measured in mouse hearts perfused in Langendorff mode...
April 14, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28405910/omics-analysis-of-acetic-acid-tolerance-in-saccharomyces-cerevisiae
#5
REVIEW
Peng Geng, Liang Zhang, Gui Yang Shi
Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the yeast responses to acetic acid stress is essential for improving acetic acid tolerance and ethanol production. Although 329 genes associated with acetic acid tolerance have been identified in the Saccharomyces genome and included in the database ( http://www...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28400329/deletion-of-type-i-glutamine-synthetase-deregulates-nitrogen-metabolism-and-increases-ethanol-production-in-clostridium-thermocellum
#6
Thomas Rydzak, David Garcia, David M Stevenson, Margaret Sladek, Dawn M Klingeman, Evert K Holwerda, Daniel Amador-Noguez, Steven D Brown, Adam M Guss
Clostridium thermocellum rapidly deconstructs cellulose and ferments resulting hydrolysis products into ethanol and other products, and is thus a promising platform organism for the development of cellulosic biofuel production via consolidated bioprocessing. While recent metabolic engineering strategies have targeted eliminating canonical fermentation products (acetate, lactate, formate, and H2), C. thermocellum also secretes amino acids, which has limited ethanol yields in engineered strains to approximately 70% of the theoretical maximum...
April 8, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28381824/metabolomics-reveals-biotic-and-abiotic-elicitor-effects-on-the-soft-coral-sarcophyton-ehrenbergi-terpenoid-content
#7
Mohamed A Farag, Dalia A Al-Mahdy, Achim Meyer, Hildegard Westphal, Ludger A Wessjohann
The effects of six biotic and abiotic elicitors, i.e. MeJA (methyl jasmonate), SA (salicylic acid), ZnCl2, glutathione and β-glucan BG (fungal elicitor), and wounding, on the secondary metabolite accumulation in the soft coral Sarcophyton ehrenbergi were assessed. Upon elicitation, metabolites were extracted and analysed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Except for MeJA, no differences in photosynthetic efficiency were observed after treatments, suggesting the absence of a remarkable stress on primary production...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28381091/differential-off-line-lc-nmr-dolc-nmr-metabolomics-to-monitor-tyrosine-induced-metabolome-alterations-in-saccharomyces-cerevisiae
#8
Richard Hammerl, Oliver Frank, Thomas Hofmann
A novel differential off-line LC-NMR approach (DOLC-NMR) was developed to capture and quantify nutrient-induced metabolome alterations in Saccharomyces cerevisiae. Off-line coupling of HPLC separation and (1)H NMR spectroscopy supported by automated comparative bucket analyses, followed by quantitative (1)H NMR using ERETIC 2 (electronic reference to access in vivo concentrations), has been successfully used to quantitatively record changes in the metabolome of S. cerevisiae upon intervention with the aromatic amino acid l-tyrosine...
April 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28372238/metabolite-profiling-and-volatiles-of-pineapple-wine-and-vinegar-obtained-from-pineapple-waste
#9
Arianna Roda, Luigi Lucini, Fabrizio Torchio, Roberta Dordoni, Dante Marco De Faveri, Milena Lambri
Vinegar is an inexpensive commodity, and economic considerations require that a relatively low-cost raw material be used for its production. An investigation into the use of a new, alternative substrate - pineapple waste - is described. This approach enables the utilization of the pineapple's (Ananas comosus) peels and core, which are usually discarded during the processing or consumption of the fruit. Using physical and enzymatic treatments, the waste was saccharified, and the resulting substrate was fermented with Saccharomyces cerevisiae for 7-10days under aerobic conditions at 25°C...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28360096/the-microbiome-of-professional-athletes-differs-from-that-of-more-sedentary-subjects-in-composition-and-particularly-at-the-functional-metabolic-level
#10
Wiley Barton, Nicholas C Penney, Owen Cronin, Isabel Garcia-Perez, Michael G Molloy, Elaine Holmes, Fergus Shanahan, Paul D Cotter, Orla O'Sullivan
OBJECTIVE: It is evident that the gut microbiota and factors that influence its composition and activity effect human metabolic, immunological and developmental processes. We previously reported that extreme physical activity with associated dietary adaptations, such as that pursued by professional athletes, is associated with changes in faecal microbial diversity and composition relative to that of individuals with a more sedentary lifestyle. Here we address the impact of these factors on the functionality/metabolic activity of the microbiota which reveals even greater separation between exercise and a more sedentary state...
March 30, 2017: Gut
https://www.readbyqxmd.com/read/28358223/ameliorative-effects-of-pyrazinoic-acid-against-oxidative-and-metabolic-stress-manifested-in-rats-with-dimethylhydrazine-induced-colonic-carcinoma
#11
Anil K Sahdev, Vinit Raj, Ashok K Singh, Amit Rai, Amit K Keshari, Arnab De, Amalesh Samanta, Umesh Kumar, Atul Rawat, Dinesh Kumar, Sneha Nath, Anand Prakash, Sudipta Saha
Pyrazinoic acid (PA) is structurally similar to nicotinic acid which acts on G-protein-coupled receptor (GPR109A). GPR109A expresses in colonic and intestinal epithelial sites, and involves in DNA methylation and cellular apoptosis. Therefore, it may be assumed that PA has similar action like nicotinic acid and may be effective against colorectal carcinoma (CRC). CRC was produced via subcutaneous injection of dimethylhydrazine (DMH) at 40 mg/kg body weight once in a week for four weeks. After that, PA was administered orally at two doses of 10 and 25 mg/kg daily for 15 days to observe the antiproliferative effect...
March 30, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28342863/disturbed-tryptophan-metabolism-correlating-to-progression-and-metastasis-of-esophageal-squamous-cell-carcinoma
#12
Jing Cheng, Hai Jin, Xiaobei Hou, Jie Lv, Xianfu Gao, Guangyong Zheng
Esophageal squamous cell carcinoma (ESCC) is one of the most frequent malignancies worldwide. Lymph node metastasis is the leading cause of death in ESCC patients. To identify early diagnostic and prognostic biomarkers of ESCC and elucidate underlying pathogenesis of the disease, a targeted metabolomics strategy based on liquid chromatography combined with tandem mass spectrometry was applied to explore tryptophan metabolism between ESCC patients, metastatic ESCC patients (mESCC), and healthy controls. Statistical analysis on metabolite expression abundance and compound concentration ratio was conducted to discriminate patients from healthy controls...
March 23, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28337503/gut-microbiome-related-metabolic-changes-in-plasma-of-antibiotic-treated-rats
#13
C Behr, H Kamp, E Fabian, G Krennrich, W Mellert, E Peter, V Strauss, T Walk, I M C M Rietjens, B van Ravenzwaay
The intestinal microbiota contributes to the metabolism of its host. Adequate identification of the microbiota's impact on the host plasma metabolites is lacking. As antibiotics have a profound effect on the microbial composition and hence on the mammalian-microbiota co-metabolism, we studied the effects of antibiotics on the "functionality of the microbiome"-defined as the production of metabolites absorbed by the host. This metabolomics study presents insights into the mammalian-microbiome co-metabolism of endogenous metabolites...
March 23, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28335380/vitamin-e-nicotinate
#14
REVIEW
Kimbell R Duncan, Yuichiro J Suzuki
Vitamin E refers to a family of compounds that function as lipid-soluble antioxidants capable of preventing lipid peroxidation. Naturally occurring forms of vitamin E include tocopherols and tocotrienols. Vitamin E in dietary supplements and fortified foods is often an esterified form of α-tocopherol, the most common esters being acetate and succinate. The vitamin E esters are hydrolyzed and converted into free α-tocopherol prior to absorption in the intestinal tract. Because its functions are relevant to many chronic diseases, vitamin E has been extensively studied in respect to a variety of diseases as well as cosmetic applications...
March 13, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28315126/screening-for-main-components-associated-with-the-idiosyncratic-hepatotoxicity-of-a-tonic-herb-polygonum-multiflorum
#15
Chunyu Li, Ming Niu, Zhaofang Bai, Congen Zhang, Yanling Zhao, Ruiyu Li, Can Tu, Huifang Li, Jing Jing, Yakun Meng, Zhijie Ma, Wuwen Feng, Jinfa Tang, Yun Zhu, Jinjie Li, Xiaoya Shang, Zhengsheng Zou, Xiaohe Xiao, Jiabo Wang
The main constituents of a typical medicinal herb, Polygonum multiflorum (Heshouwu in Chinese), that induces idiosyncratic liver injury remain unclear. Our previous work has shown that cotreatment with a nontoxic dose of lipopolysaccharide (LPS) and therapeutic dose of Heshouwu can induce liver injury in rats, whereas the solo treatment cannot induce observable injury. In the present work, using the constituent "knock-out" and "knock-in" strategy, we found that the ethyl acetate (EA) extract of Heshouwu displayed comparable idiosyncratic hepatotoxicity to the whole extract in LPS-treated rats...
March 17, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28286172/age-related-changes-in-skeletal-muscle-composition-a-pilot-nuclear-magnetic-resonance-spectroscopy-study-in-mice
#16
Anatoly P Sobolev, Luisa Mannina, Manuela Costanzo, Barbara Cisterna, Manuela Malatesta, Carlo Zancanaro
The composition of skeletal muscle was investigated in the quadriceps and gastrocnemius muscle of 13-month-old (n=15) and 23-month-old (n=19) mice by means of high-resolution nuclear magnetic resonance (NMR) spectroscopy. Muscle specimens were dissected out, frozen in liquid nitrogen and extracted in chloroform/methanol, and proton NMR spectra of the resulting aqueous and organic fractions were obtained at 600MHz. Several metabolites were unambiguously identified and quantified. Multivariate ANOVA (factor: age, muscle, age×muscle) showed a significant main effect of age (P=0...
June 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28285599/microbiome-and-metabolome-modifying-effects-of-several-cardiovascular-disease-interventions-in-apo-e-mice
#17
Paul M Ryan, Lis E E London, Trent C Bjorndahl, Rupasri Mandal, Kiera Murphy, Gerald F Fitzgerald, Fergus Shanahan, R Paul Ross, David S Wishart, Noel M Caplice, Catherine Stanton
BACKGROUND: There is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon. This study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches. Apolipoprotein-E-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (control, HFC) or high-fat/cholesterol in conjunction with one of three dietary interventions, as follows: plant sterol ester (PSE), oat β-glucan (OBG) and bile salt hydrolase-active Lactobacillus reuteri APC 2587 (BSH), or the drug atorvastatin (STAT)...
March 13, 2017: Microbiome
https://www.readbyqxmd.com/read/28280764/lipid-quantitation-and-metabolomics-data-from-vitamin-e-deficient-and-sufficient-zebrafish-embryos-from-0-to-120-hours-post-fertilization
#18
Melissa McDougall, Jaewoo Choi, Hye-Kyeong Kim, Gerd Bobe, J Frederik Stevens, Enrique Cadenas, Robert Tanguay, Maret G Traber
The data herein is in support of our research article by McDougall et al. (2017) [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E-) or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet), were spawned to obtain E- and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28272319/facilitated-visual-interpretation-of-scores-in-principal-component-analysis-by-bioactivity-labeling-of-1h-nmr-spectra-metabolomics-investigation-and-identification-of-a-new-%C3%AE-glucosidase-inhibitor-in-radix-astragali
#19
Yueqiu Liu, Nils T Nyberg, Anna K Jäger, Dan Staerk
Radix Astragali is a component of several traditional medicines used for the treatment of type 2 diabetes in China. Radix Astragali is known to contain isoflavones, which inhibit α-glucosidase in the small intestines, and thus lowers the blood glucose levels. In this study, 21 samples obtained from different regions of China were extracted with ethyl acetate, then the IC50-values were determined, and the crude extracts were analyzed by 1H-NMR spectroscopy. A principal component analysis of the 1H-NMR spectra labeled with their IC50-values, that is, bioactivity-labeled 1H-NMR spectra, showed a clear correlation between spectral profiles and the α-glucosidase inhibitory activity...
March 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28265055/glyoxylate-detoxification-is-an-essential-function-of-malate-synthase-required-for-carbon-assimilation-in-mycobacterium-tuberculosis
#20
Susan Puckett, Carolina Trujillo, Zhe Wang, Hyungjin Eoh, Thomas R Ioerger, Inna Krieger, James Sacchettini, Dirk Schnappinger, Kyu Y Rhee, Sabine Ehrt
The glyoxylate shunt is a metabolic pathway of bacteria, fungi, and plants used to assimilate even-chain fatty acids (FAs) and has been implicated in persistence of Mycobacterium tuberculosis (Mtb). Recent work, however, showed that the first enzyme of the glyoxylate shunt, isocitrate lyase (ICL), may mediate survival of Mtb during the acute and chronic phases of infection in mice through physiologic functions apart from fatty acid metabolism. Here, we report that malate synthase (MS), the second enzyme of the glyoxylate shunt, is essential for in vitro growth and survival of Mtb on even-chain fatty acids, in part, for a previously unrecognized activity: mitigating the toxicity of glyoxylate excess arising from metabolism of even-chain fatty acids...
March 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
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