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Metabolomic dialysis

Akiyoshi Hirayama, Hiroshi Abe, Nozomi Yamaguchi, Sho Tabata, Masaru Tomita, Tomoyoshi Soga
A sheath-flow interface is the most common ionization technique in CE-ESI-MS. However, this interface dilutes the analytes with the sheath liquid and decreases the sensitivity. In this study, we developed a sheathless CE-MS interface to improve sensitivity. The interface was fabricated by making small crack approximately 2 cm from the end of a capillary column fixed on a plastic plate, and then covering the crack with a dialysis membrane to prevent metabolite loss during separation. A voltage for CE separation was applied between the capillary inlet and the buffer reservoir...
March 1, 2018: Electrophoresis
Sahir Kalim, Ron Wald, Andrew T Yan, Marc B Goldstein, Mercedeh Kiaii, Dihua Xu, Anders H Berg, Clary Clish, Ravi Thadhani, Eugene P Rhee, Jeffrey Perl
BACKGROUND AND OBJECTIVES: In-center, extended duration nocturnal hemodialysis has been associated with variable clinical benefits, but the effect of extended duration hemodialysis on many established uremic solutes and other components of the metabolome is unknown. We determined the magnitude of change in metabolite profiles for patients on extended duration nocturnal hemodialysis. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: In a 52-week prospective, observational study, we followed 33 patients receiving conventional thrice weekly hemodialysis who converted to nocturnal hemodialysis (7-8 hours per session, three times per week)...
February 14, 2018: Clinical Journal of the American Society of Nephrology: CJASN
Vijaya B Kolachalama, Moshe Shashar, Faisal Alousi, Sowmya Shivanna, Keshab Rijal, Mostafa E Belghasem, Joshua Walker, Shinobu Matsuura, Gary H Chang, C Michael Gibson, Laura M Dember, Jean M Francis, Katya Ravid, Vipul C Chitalia
Individuals with CKD are particularly predisposed to thrombosis after vascular injury. Using mouse models, we recently described indoxyl sulfate, a tryptophan metabolite retained in CKD and an activator of tissue factor (TF) through aryl hydrocarbon receptor (AHR) signaling, as an inducer of thrombosis across the CKD spectrum. However, the translation of findings from animal models to humans is often challenging. Here, we investigated the uremic solute-AHR-TF thrombosis axis in two human cohorts, using a targeted metabolomics approach to probe a set of tryptophan products and high-throughput assays to measure AHR and TF activity...
January 17, 2018: Journal of the American Society of Nephrology: JASN
Shuxin Liu, Lichao Wang, Chunxiu Hu, Xin Huang, Hong Liu, Qiuhui Xuan, Xiaohui Lin, Xiaojun Peng, Xin Lu, Ming Chang, Guowang Xu
Uremia has been a rapidly increasing health problem in China. Hemodialysis (HD) is the main renal replacement therapy for uremia. The results of large-scale clinical trials have shown that the HD pattern is crucial for long-term prognosis of maintenance hemodialysis (MHD) in uremic patients. Plasma metabolism is very important for revealing the biological insights linked to the therapeutic effects of the HD pattern on uremia. Alteration of plasma metabolites in uremic patients in response to HD therapy has been reported...
August 15, 2017: Scientific Reports
Tammy L Sirich, Kara Fong, Brett Larive, Gerald J Beck, Glenn M Chertow, Nathan W Levin, Alan S Kliger, Natalie S Plummer, Timothy W Meyer
The Frequent Hemodialysis Network Daily Trial compared conventional three-times weekly treatment to more frequent treatment with a longer weekly treatment time in patients receiving in-center hemodialysis. Evaluation at one year showed favorable effects of more intensive treatment on left ventricular mass, blood pressure, and phosphate control, but modest or no effects on physical or cognitive performance. The current study compared plasma concentrations of uremic solutes in stored samples from 53 trial patients who received three-times weekly in-center hemodialysis for an average weekly time of 10...
May 2017: Kidney International
Klaus Kratochwill, Michael Boehm, Rebecca Herzog, Katharina Gruber, Anton Michael Lichtenauer, Lilian Kuster, Dagmar Csaicsich, Andreas Gleiss, Seth L Alper, Christoph Aufricht, Andreas Vychytil
BACKGROUND: Peritonitis and ultrafiltration failure remain serious complications of chronic peritoneal dialysis (PD). Dysfunctional cellular stress responses aggravate peritoneal injury associated with PD fluid exposure, potentially due to peritoneal glutamine depletion. In this randomized cross-over phase I/II trial we investigated cytoprotective effects of alanyl-glutamine (AlaGln) addition to glucose-based PDF. METHODS: In a prospective randomized cross-over design, 20 stable PD outpatients underwent paired peritoneal equilibration tests 4 weeks apart, using conventional acidic, single chamber 3...
2016: PloS One
Jueun Lee, Ji-Young Choi, Yong-Kook Kwon, Doohae Lee, Hee-Yeon Jung, Hye-Myung Ryu, Jang-Hee Cho, Do Hyun Ryu, Yong-Lim Kim, Geum-Sook Hwang
BACKGROUND: The renal dysfunction of chronic kidney disease (CKD) alters serum metabolite levels, but it is not clear how diabetes mellitus (DM) affects the metabolic changes in CKD. METHODS: Serum metabolites from pre-dialysis CKD patients (n=291) with or without DM and from healthy controls (n=56) was measured using nuclear magnetic resonance. RESULTS: Initial principal components analysis and partial least squares-discriminant analysis score plots segregated the CKD patients according to CKD stage and separated DM from non-DM patients...
August 1, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
I Barchetta, D Capoccia, M G Baroni, R Buzzetti, M G Cavallo, S De Cosmo, F Leonetti, S Leotta, S Morano, L Morviducci, S Prudente, G Pugliese, V Trischitta
BACKGROUND AND AIMS: The rate of mortality in diabetic patients, especially of cardiovascular origin, is about twice as much that of nondiabetic individuals. Thus, the pathogenic factors shaping the risk of mortality in such patients must be unraveled in order to target intensive prevention and treatment strategies. The "Sapienza University Mortality and Morbidity Event Rate (SUMMER) study in diabetes" is aimed at identifying new molecular promoters of mortality and major vascular events in patients with type 2 diabetes mellitus (T2DM)...
February 2016: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
Marie-Aleth Lacaille-Dubois
This review will focus on newest results leading to the discovery of new bioactive saponins by using a combination of successive advanced procedures in extraction, isolation, structure elucidation and bioassays. Microwave- and ultrasonic-assisted extractions, two recent advanced methods have been increasingly used in the last decade. Then, a multistep purification procedure was achieved by flash chromatography, vacuum liquid chromatography, low, medium- and high-pressure liquid chromatography on silica gel and reversed-phase silica gel RP-18 (VLC, LPLC, MPLC, HPLC)...
2016: Current Drug Delivery
Sahir Kalim, Clary B Clish, Joseph J Deferio, Guillermo Ortiz, Alexander S Moffet, Robert E Gerszten, Ravi Thadhani, Eugene P Rhee
BACKGROUND: Although metabolomic approaches have begun to document numerous changes that arise in end stage renal disease (ESRD), how these alterations relate to established metabolic phenotypes in uremia is unknown. METHODS: In 200 incident hemodialysis patients we used partial least squares discriminant analysis to identify which among 166 metabolites could best discriminate individuals with or without diabetes, and across tertiles of body mass index, serum albumin, total cholesterol, and systolic blood pressure...
July 7, 2015: BMC Nephrology
Olivier Devuyst, Anke Schumann
No abstract text is available yet for this article.
May 2015: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
Laurent Weekers, Pascal de Tullio, Christophe Bovy, Laurence Poma, Raphaël Marée, Catherine Bonvoisin, Jean-Olivier Defraigne, Jean-Marie Krzesinski, François Jouret
Activation of the calcium-sensing receptor (CaSR) by ischemia/reperfusion (I/R) favours apoptosis in cardiomyocytes, hepatocytes and neurons. Its role in renal I/R is unknown. We investigated the impact of pharmacological preactivation of the CaSR on kidney structure and function in a murine model of bilateral renal 30-min ischemia and 48-hour reperfusion, and in a 6-year cohort of kidney transplant recipients (KTR). C57BL/6J mice were administered daily with CaSR agonist, R-568, or with vehicle for 48 hours...
2015: American Journal of Translational Research
Raymond Vanholder, Jente Boelaert, Griet Glorieux, Sunny Eloot
This publication reviews the currently available methods to identify uremic retention solutes, to determine their biological relevance and to quantify their removal. The analytical methods for the detection of uremic solutes have improved continuously, allowing the identification of several previously unknown solutes. Progress has been accelerated by the development of comprehensive strategies such as genomics, proteomics and the latest "omics" area, metabolomics. Those methodologies will be further refined in future...
March 2015: Seminars in Dialysis
Vincent Forster, Rea Deborah Signorell, Maurizio Roveri, Jean-Christophe Leroux
Peritoneal dialysis confers therapeutic advantages in patients with renal insufficiency and has proven beneficial in other indications, such as removal of excess metabolites or overdosed drugs. However, it is used in only about 10% of the dialyzed population worldwide, partly owing to the lower clearance rate compared with hemodialysis. We have developed a dialysis medium based on liposomes with a transmembrane pH gradient (basic or acidic aqueous core) that could improve the efficacy of peritoneal dialysis, specifically for the removal of excess metabolites or overdosed drugs...
October 15, 2014: Science Translational Medicine
Christian Henning, Kristin Liehr, Matthias Girndt, Christof Ulrich, Marcus A Glomb
Maillard α-dicarbonyl compounds are known as central intermediates in advanced glycation end product (AGE) formation. Glucose is the primary source of energy for the human body, whereas l-threo-ascorbic acid (vitamin C) is an essential nutrient, involved in a variety of enzymatic reactions. Thus, the Maillard degradation of glucose and ascorbic acid is of major importance in vivo. To understand the complex mechanistic pathways of AGE formation, it is crucial to extend the knowledge on plasma concentrations of reactive key α-dicarbonyl compounds (e...
October 10, 2014: Journal of Biological Chemistry
William Mullen, Daisuke Saigusa, Takaaki Abe, Jerzy Adamski, Harald Mischak
The development of proteomic and metabolomic technologies holds the promise to significantly impact patient management by improving diagnosis, unraveling more appropriate therapeutic targets, and enabling more precise prognosis of disease development. Proteomics and metabolomics have been applied with the aim of improving dialysis, defining uremic toxins, and unraveling their origin. Ideally, these technologies should inform us which proteomic or metabolomic compounds are subject to significant alterations of concentration or structure as a result of failing kidney function, and thus can be considered as potential uremic toxins...
March 2014: Seminars in Nephrology
Sahir Kalim, Clary B Clish, Julia Wenger, Sammy Elmariah, Robert W Yeh, Joseph J Deferio, Kerry Pierce, Amy Deik, Robert E Gerszten, Ravi Thadhani, Eugene P Rhee
BACKGROUND: The marked excess in cardiovascular mortality that results from uremia remains poorly understood. METHODS AND RESULTS: In 2 independent, nested case-control studies, we applied liquid chromatography-mass spectrometry-based metabolite profiling to plasma obtained from participants of a large cohort of incident hemodialysis patients. First, 100 individuals who died of a cardiovascular cause within 1 year of initiating hemodialysis (cases) were randomly selected along with 100 individuals who survived for at least 1 year (controls), matched for age, sex, and race...
2013: Journal of the American Heart Association
Flore Duranton, Ulrika Lundin, Nathalie Gayrard, Harald Mischak, Michel Aparicio, Georges Mourad, Jean-Pierre Daurès, Klaus M Weinberger, Angel Argilés
BACKGROUND AND OBJECTIVES: Patients with CKD display altered plasma amino acid profiles. This study estimated the association between the estimated GFR and urinary and plasma amino acid profiles in CKD patients. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Urine and plasma samples were taken from 52 patients with different stages of CKD, and plasma samples only were taken from 25 patients on maintenance hemodialysis. Metabolic profiling was performed by liquid chromatography coupled with tandem mass spectrometry after phenylisothiocyanate derivatization...
January 2014: Clinical Journal of the American Society of Nephrology: CJASN
Mei-Ju Ko, Hon-Yen Wu, Hung-Yuan Chen, Yen-Ling Chiu, Shih-Ping Hsu, Mei-Fen Pai, Ju-Yehyang, Chun-Fu Lai, Hui-Min Lu, Shu-Chen Huang, Shao-Yu Yang, Su-Yin Wen, Hsien-Ching Chiu, Fu-Chang Hu, Yu-Sen Peng, Shiou-Hwa Jee
BACKGROUND: Uremic pruritus is a common and intractable symptom in patients on chronic hemodialysis, but factors associated with the severity of pruritus remain unclear. This study aimed to explore the associations of metabolic factors and dialysis adequacy with the aggravation of pruritus. METHODS: We conducted a 5-year prospective cohort study on patients with maintenance hemodialysis. A visual analogue scale (VAS) was used to assess the intensity of pruritus...
2013: PloS One
Henricus A M Mutsaers, Udo F H Engelke, Martijn J G Wilmer, Jack F M Wetzels, Ron A Wevers, Lambertus P van den Heuvel, Joost G Hoenderop, Rosalinde Masereeuw
BACKGROUND: Chronic kidney disease (CKD) is characterized by the progressive accumulation of various potential toxic solutes. Furthermore, uremic plasma is a complex mixture hampering accurate determination of uremic toxin levels and the identification of novel uremic solutes. METHODS: In this study, we applied (1)H-nuclear magnetic resonance (NMR) spectroscopy, following three distinct deproteinization strategies, to determine differences in the plasma metabolic status of stage 3-4 CKD patients and healthy controls...
2013: PloS One
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