keyword
MENU ▼
Read by QxMD icon Read
search

PANK

keyword
https://www.readbyqxmd.com/read/28585362/tailor-made-pat-platform-for-safe-syngas-fermentations-in-batch-fed-batch-and-chemostat-mode-with-rhodospirillum-rubrum
#1
Stephanie Karmann, Stéphanie Follonier, Daniel Egger, Dirk Hebel, Sven Panke, Manfred Zinn
Recently, syngas has gained significant interest as renewable and sustainable feedstock, in particular for the biotechnological production of poly([R]-3-hydroxybutyrate) (PHB). PHB is a biodegradable, biocompatible polyester produced by some bacteria growing on the principal component of syngas, CO. However, working with syngas is challenging because of the CO toxicity and the explosion danger of H2 , another main component of syngas. In addition, the bioprocess control needs specific monitoring tools and analytical methods that differ from standard fermentations...
June 6, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28534959/microrna-200c-regulates-cisplatin-resistance-by-targeting-zeb2-in-human-gastric-cancer-cells
#2
Tao Jiang, Pengfei Dong, Long Li, Xiao Ma, Pei Xu, He Zhu, Yanqiu Wang, Baotong Yang, Kuangge Liu, Jinwei Liu, Juan Xue, Runzhe Lv, Panke Su, Guoqiang Kong, Yongchao Chang, Chonggao Zhao, Lidong Wang
This study was specifically designed to confirm the hypothesis that microRNA-200c (miR-200c) affects the development of cisplatin (DDP) resistance in human gastric cancer cells by targeting zinc finger E-box binding homeobox 2 (ZEB2). A total of 50 gastric cancer tissues and their corresponding normal adjacent tissue samples were collected. Then, the expression levels of miR-200c and ZEB2 in both gastric cancer specimens and cells were detected using the quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical methods...
May 22, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28441142/magnetization-dynamics-in-magnonic-structures-with-different-geometries-interfaces-notches-and-waveguides
#3
M Mansurova, J von der Haar, J Panke, Jakob Walowski, Henning Ulrichs, M Münzenberg
The discovery of ultrafast magnetization dynamics 20 years ago has led to a broad variety of experimental techniques to explore phenomena in magnetic materials with high temporal resolution. In the current article we present a study dealing with broadband excitation of spin-wave packets at different magnonic crystal continuous magnetic film interfaces. Similar to protected conducting states on the surfaces of topological band insulators, these interfaces exhibit surface spin-wave modes that propagate out of the crystal into the continuous film...
June 1, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28438135/a-passive-mutualistic-interaction-promotes-the-evolution-of-spatial-structure-within-microbial-populations
#4
Marie Marchal, Felix Goldschmidt, Selina N Derksen-Müller, Sven Panke, Martin Ackermann, David R Johnson
BACKGROUND: While mutualistic interactions between different genotypes are pervasive in nature, their evolutionary origin is not clear. The dilemma is that, for mutualistic interactions to emerge and persist, an investment into the partner genotype must pay off: individuals of a first genotype that invest resources to promote the growth of a second genotype must receive a benefit that is not equally accessible to individuals that do not invest. One way for exclusive benefits to emerge is through spatial structure (i...
April 24, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28346794/comparative-study-of-xenobiotic-free-media-for-the-cultivation-of-human-limbal-epithelial-stem-progenitor-cells
#5
Sheyla González, Luxia Chen, Sophie X Deng
The culture of human limbal epithelial stem/progenitor cells (LSCs) in the presence of animal components poses the risk of cross-species contamination in clinical applications. We quantitatively compared different xenobiotic-free culture media for the cultivation of human LSCs. LSCs were cultured from 2 × 2 mm limbal tissue explants on denuded human amniotic membrane with different xenobiotic-free culture media: CnT-Prime (CnT-PR) supplemented with 0%, 1%, 5%, and 10% human serum (HS), embryonic stem cell medium (ESCM) alone or in combination with the standard supplemented hormonal epithelium medium (SHEM, control) at a 1:1 dilution ratio, and modified SHEM (mSHEM), in which cholera toxin and dimethyl sulfoxide (DMSO) were removed, isoproterenol was added, and the epidermal growth factor concentration was reduced...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28284103/a-vegf-delivery-system-targeting-mi-improves-angiogenesis-and-cardiac-function-based-on-the-tropism-of-mscs-and-layer-by-layer-self-assembly
#6
Ge Liu, Li Li, Da Huo, Yanzhao Li, Yangxiao Wu, Lingqing Zeng, Panke Cheng, Malcolm Xing, Wen Zeng, Chuhong Zhu
Myocardial infarction (MI) is a serious ischemic condition affecting many individuals around the world. Vascular endothelial growth factor (VEGF) is considered a promising factor for enhancing cardiac function by promoting angiogenesis. However, the lack of a suitable method of VEGF delivery to the MI area is a serious challenge. In this study, we screened a suitable delivery carrier with favorable biocompatibility that targeted the MI area using the strategy of an inherent structure derived from the body and that was based on characteristics of the MI...
March 2, 2017: Biomaterials
https://www.readbyqxmd.com/read/28283403/genome-surfing-as-driver-of-microbial-genomic-diversity
#7
REVIEW
Mallory J Choudoir, Kevin Panke-Buisse, Cheryl P Andam, Daniel H Buckley
Historical changes in population size, such as those caused by demographic range expansions, can produce nonadaptive changes in genomic diversity through mechanisms such as gene surfing. We propose that demographic range expansion of a microbial population capable of horizontal gene exchange can result in genome surfing, a mechanism that can cause widespread increase in the pan-genome frequency of genes acquired by horizontal gene exchange. We explain that patterns of genetic diversity within Streptomyces are consistent with genome surfing, and we describe several predictions for testing this hypothesis both in Streptomyces and in other microorganisms...
March 7, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28243574/5-year-imaging-sequelae-of-concussive-blast-injury-and-relation-to-early-clinical-outcome
#8
Christine L Mac Donald, Jason Barber, Jalal Andre, Nicole Evans, Chris Panks, Samantha Sun, Kody Zalewski, R Elizabeth Sanders, Nancy Temkin
Current imaging diagnostic techniques are often insensitive to the underlying pathological changes following mild traumatic brain injury (TBI) or concussion so much so that the explicit definition of these uncomplicated mild brain injuries includes the absence of radiological findings. In the US military, this is complicated by the natural tendency of service members to down play symptoms for fear of removal from their unit particularly in combat making it challenging for clinicians to definitively diagnose and determine course of treatment...
2017: NeuroImage: Clinical
https://www.readbyqxmd.com/read/28239413/improving-acetyl-coa-biosynthesis-in-saccharomyces-cerevisiae-via-the-overexpression-of-pantothenate-kinase-and-pdh-bypass
#9
Wenshan Liu, Bo Zhang, Rongrong Jiang
BACKGROUND: Acetyl-CoA is an important precursor in Saccharomyces cerevisiae. Various approaches have been adopted to improve its cytosolic level previously with the emphasis on engineering the "acetyl-" part of acetyl-CoA. To the best of our knowledge, there have been no reports on engineering the "-CoA" part so far. RESULTS: In this study, we had tried to engineer S. cerevisiae from both the "-CoA" part via pantothenate kinase overexpression (PanK from S. cerevisiae, the rate-limiting enzyme for CoA synthesis) and the "acetyl-"part through PDH bypass introduction (ALD6 from S...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28062280/biotin-independent-strains-of-escherichia-coli-for-enhanced-streptavidin-production
#10
Markus Jeschek, Maximilian O Bahls, Veronika Schneider, Philippe Marlière, Thomas R Ward, Sven Panke
Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supplement defined media. Here we describe the creation of biotin-independent strains of Escherichia coli and Corynebacterium glutamicum through installation of an optimized malonyl-CoA bypass, which re-routes natural fatty acid synthesis, rendering the previously essential vitamin completely obsolete...
January 3, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/27989807/overcoming-the-membrane-barrier-recruitment-of-%C3%AE-glutamyl-transferase-for-intracellular-release-of-metabolic-cargo-from-peptide-vectors
#11
Tilmann Kuenzl, Magdalena Sroka, Puneet Srivastava, Piet Herdewijn, Philippe Marlière, Sven Panke
Semipermeable membranes of cells frequently pose an obstacle in metabolic engineering by limiting uptake of substrates, intermediates, or xenobiotics. Previous attempts to overcome this barrier relied on the promiscuous nature of peptide transport systems, but often suffered from low versatility or chemical instability. Here, we present an alternative strategy to transport cargo molecules across the inner membrane of Escherichia coli based on chemical synthesis of a stable cargo-peptide vector construct, transport through the peptide import system, and efficient intracellular release of the cargo by the promiscuous enzyme γ-glutamyl transferase (GGT)...
January 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/27981013/plga-pnipam-microspheres-loaded-with-the-gastrointestinal-nutrient-nab-ameliorate-cardiac-dysfunction-by-activating-sirt3-in-acute-myocardial-infarction
#12
Panke Cheng, Wen Zeng, Li Li, Da Huo, Lingqing Zeng, Ju Tan, Jingting Zhou, Jiansen Sun, Ge Liu, Yanzhao Li, Ge Guan, Yuxin Wang, Chuhong Zhu
Acute myocardial infarction (AMI) is the death of cardiomyocytes caused by a lack of energy due to ischemia. Nutrients supplied by the blood are the main source of cellular energy for cardiomyocytes. Sodium butyrate (NaB), a gastrointestinal nutrient, is a short-chain fatty acid (butyric acid) that may act as an energy source in AMI therapy. Poly(lactic-co-glycolic acid)-Poly (N-isopropylacrylamide) microspheres loaded with NaB (PP-N) are synthesized to prolong the release of NaB and are injected into ischemic zones in a Sprague-Dawley rat AMI model...
December 2016: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/27862817/design-of-s-allylcysteine-in-situ-production-and-incorporation-based-on-a-novel-pyrrolysyl-trna-synthetase-variant
#13
Matthias P Exner, Tilmann Kuenzl, Tuyet Mai T To, Zhaofei Ouyang, Sergej Schwagerus, Michael G Hoesl, Christian P R Hackenberger, Marga C Lensen, Sven Panke, Nediljko Budisa
The noncanonical amino acid S-allyl cysteine (Sac) is one of the major compounds of garlic extract and exhibits a range of biological activities. It is also a small bioorthogonal alkene tag capable of undergoing controlled chemical modifications, such as photoinduced thiol-ene coupling or Pd-mediated deprotection. Its small size guarantees minimal interference with protein structure and function. Here, we report a simple protocol efficiently to couple in-situ semisynthetic biosynthesis of Sac and its incorporation into proteins in response to amber (UAG) stop codons...
January 3, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27759386/discovery-of-potent-pantothenamide-inhibitors-of-staphylococcus-aureus-pantothenate-kinase-through-a-minimal-sar-study-inhibition-is-due-to-trapping-of-the-product
#14
Scott J Hughes, Leanne Barnard, Katayoun Mottaghi, Wolfram Tempel, Tetyana Antoshchenko, Bum Soo Hong, Abdellah Allali-Hassani, David Smil, Masoud Vedadi, Erick Strauss, Hee-Won Park
The potent antistaphylococcal activity of N-substituted pantothenamides (PanAms) has been shown to at least partially be due to the inhibition of Staphylococcus aureus's atypical type II pantothenate kinase (SaPanKII), the first enzyme of coenzyme A biosynthesis. This mechanism of action follows from SaPanKII having a binding mode for PanAms that is distinct from those of other PanKs. To dissect the molecular interactions responsible for PanAm inhibitory activity, we conducted a mini SAR study in tandem with the cocrystallization of SaPanKII with two classic PanAms (N5-Pan and N7-Pan), culminating in the synthesis and characterization of two new PanAms, N-Pip-PanAm and MeO-N5-PanAm...
September 9, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27757475/synthetic-biology-for-cell-free-biosynthesis-fundamentals-of-designing-novel-in-vitro-multi-enzyme-reaction-networks
#15
Gaspar Morgado, Daniel Gerngross, Tania M Roberts, Sven Panke
Cell-free biosynthesis in the form of in vitro multi-enzyme reaction networks or enzyme cascade reactions emerges as a promising tool to carry out complex catalysis in one-step, one-vessel settings. It combines the advantages of well-established in vitro biocatalysis with the power of multi-step in vivo pathways. Such cascades have been successfully applied to the synthesis of fine and bulk chemicals, monomers and complex polymers of chemical importance, and energy molecules from renewable resources as well as electricity...
October 19, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27720900/robust-at-line-quantification-of-poly-3-hydroxyalkanoate-biosynthesis-by-flow-cytometry-using-a-bodipy-493-503-syto-62-double-staining
#16
Stephanie Karmann, Stéphanie Follonier, Monica Bassas-Galia, Sven Panke, Manfred Zinn
Poly(3-hydroxyalkanoates) (PHAs) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. Their bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of carbon substrates and other nutrients. These dynamics require a fast and quantitative method to determine the optimal harvest-time of PHA containing cells or to adjust carbon supply. In principle, flow cytometry (FCM) is an ideal tool that suits these requirements and, in addition, provides data on the PHA content of different cell populations...
October 6, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27710126/recognising-and-assessing-victims-of-torture-in-emergency-departments
#17
Tricia Pank
A wall of silence surrounds torture. This silence, shame and humiliation is just what the torturer depends on. It is important to remember this when a survivor (refugee or asylum seeker) whose life has been damaged and destroyed in many ways is being assessed in the A&E department. Over 5,000 people per month seek refuge in the UK, many of whom will have suffered torture, leaving them physically and mentally injured. All will have suffered loss of loved ones, status, dignity and their homeland, and will be grieving...
May 1, 2001: Emergency Nurse: the Journal of the RCN Accident and Emergency Nursing Association
https://www.readbyqxmd.com/read/27677244/forward-design-of-a-complex-enzyme-cascade-reaction
#18
Christoph Hold, Sonja Billerbeck, Sven Panke
Enzymatic reaction networks are unique in that one can operate a large number of reactions under the same set of conditions concomitantly in one pot, but the nonlinear kinetics of the enzymes and the resulting system complexity have so far defeated rational design processes for the construction of such complex cascade reactions. Here we demonstrate the forward design of an in vitro 10-membered system using enzymes from highly regulated biological processes such as glycolysis. For this, we adapt the characterization of the biochemical system to the needs of classical engineering systems theory: we combine online mass spectrometry and continuous system operation to apply standard system theory input functions and to use the detailed dynamic system responses to parameterize a model of sufficient quality for forward design...
2016: Nature Communications
https://www.readbyqxmd.com/read/27655524/cultivated-sub-populations-of-soil-microbiomes-retain-early-flowering-plant-trait
#19
Kevin Panke-Buisse, Stacey Lee, Jenny Kao-Kniffin
The collection of microorganisms found in the root zone of soil, termed the rhizosphere microbiome, has been shown to impact plant growth and development. Here, we tease apart the function of the cultivable portion of the microbiome from the whole microbiome in retaining plant traits modified through artificial selection on flowering time. Specifically, the whole microbiome associated with earlier flowering time of Arabidopsis thaliana was cultivated on four types of solid media to create cultivated fractions of the microbiome...
February 2017: Microbial Ecology
https://www.readbyqxmd.com/read/27587179/note-electron-energy-spectroscopy-mapping-of-surface-with-scanning-tunneling-microscope
#20
Meng Li, Chunkai Xu, Panke Zhang, Zhean Li, Xiangjun Chen
We report a novel scanning probe electron energy spectrometer (SPEES) which combines a double toroidal analyzer with a scanning tunneling microscope to achieve both topography imaging and electron energy spectroscopy mapping of surface in situ. The spatial resolution of spectroscopy mapping is determined to be better than 0.7 ± 0.2 μm at a tip sample distance of 7 μm. Meanwhile, the size of the field emission electron beam spot on the surface is also measured, and is about 3.6 ± 0.8 μm in diameter. This unambiguously demonstrates that the spatial resolution of SPEES technique can be much better than the size of the incident electron beam...
August 2016: Review of Scientific Instruments
keyword
keyword
120003
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"