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https://www.readbyqxmd.com/read/27862817/design-of-s-allylcysteine-in-situ-production-and-incorporation-based-on-a-novel-pyrrolysyl-trna-synthetase-variant
#1
Matthias P Exner, Tilmann Kuenzl, Tuyet Mai T To, Zhaofei Ouyang, Sergej Schwagerus, Michael G Hoesl, Christian P R Hackenberger, Marga C Lensen, Sven Panke, Nediljko Budisa
The noncanonical amino acid S-allyl cysteine (Sac) is one of the major compounds of garlic extract and exhibits a range of biological activities. It is also a small bioorthogonal alkene tag capable of undergoing controlled chemical modifications, such as photoinduced thiol-ene coupling or Pd-mediated deprotection. Its small size guarantees minimal interference with protein structure and function. Here, we report a simple protocol efficiently to couple in-situ semisynthetic biosynthesis of Sac and its incorporation into proteins in response to amber (UAG) stop codons...
November 14, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27759386/discovery-of-potent-pantothenamide-inhibitors-of-staphylococcus-aureus-pantothenate-kinase-through-a-minimal-sar-study-inhibition-is-due-to-trapping-of-the-product
#2
Scott J Hughes, Leanne Barnard, Katayoun Mottaghi, Wolfram Tempel, Tetyana Antoshchenko, Bum Soo Hong, Abdellah Allali-Hassani, David Smil, Masoud Vedadi, Erick Strauss, Hee-Won Park
The potent antistaphylococcal activity of N-substituted pantothenamides (PanAms) has been shown to at least partially be due to the inhibition of Staphylococcus aureus's atypical type II pantothenate kinase (SaPanKII), the first enzyme of coenzyme A biosynthesis. This mechanism of action follows from SaPanKII having a binding mode for PanAms that is distinct from those of other PanKs. To dissect the molecular interactions responsible for PanAm inhibitory activity, we conducted a mini SAR study in tandem with the cocrystallization of SaPanKII with two classic PanAms (N5-Pan and N7-Pan), culminating in the synthesis and characterization of two new PanAms, N-Pip-PanAm and MeO-N5-PanAm...
September 9, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27757475/synthetic-biology-for-cell-free-biosynthesis-fundamentals-of-designing-novel-in-vitro-multi-enzyme-reaction-networks
#3
Gaspar Morgado, Daniel Gerngross, Tania M Roberts, Sven Panke
Cell-free biosynthesis in the form of in vitro multi-enzyme reaction networks or enzyme cascade reactions emerges as a promising tool to carry out complex catalysis in one-step, one-vessel settings. It combines the advantages of well-established in vitro biocatalysis with the power of multi-step in vivo pathways. Such cascades have been successfully applied to the synthesis of fine and bulk chemicals, monomers and complex polymers of chemical importance, and energy molecules from renewable resources as well as electricity...
October 19, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27720900/robust-at-line-quantification-of-poly-3-hydroxyalkanoate-biosynthesis-by-flow-cytometry-using-a-bodipy-493-503-syto-62-double-staining
#4
Stephanie Karmann, Stéphanie Follonier, Monica Bassas-Galia, Sven Panke, Manfred Zinn
Poly(3-hydroxyalkanoates) (PHAs) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. Their bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of carbon substrates and other nutrients. These dynamics require a fast and quantitative method to determine the optimal harvest-time of PHA containing cells or to adjust carbon supply. In principle, flow cytometry (FCM) is an ideal tool that suits these requirements and, in addition, provides data on the PHA content of different cell populations...
October 6, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27710126/recognising-and-assessing-victims-of-torture-in-emergency-departments
#5
Tricia Pank
A wall of silence surrounds torture. This silence, shame and humiliation is just what the torturer depends on. It is important to remember this when a survivor (refugee or asylum seeker) whose life has been damaged and destroyed in many ways is being assessed in the A&E department. Over 5,000 people per month seek refuge in the UK, many of whom will have suffered torture, leaving them physically and mentally injured. All will have suffered loss of loved ones, status, dignity and their homeland, and will be grieving...
May 1, 2001: Emergency Nurse: the Journal of the RCN Accident and Emergency Nursing Association
https://www.readbyqxmd.com/read/27677244/forward-design-of-a-complex-enzyme-cascade-reaction
#6
Christoph Hold, Sonja Billerbeck, Sven Panke
Enzymatic reaction networks are unique in that one can operate a large number of reactions under the same set of conditions concomitantly in one pot, but the nonlinear kinetics of the enzymes and the resulting system complexity have so far defeated rational design processes for the construction of such complex cascade reactions. Here we demonstrate the forward design of an in vitro 10-membered system using enzymes from highly regulated biological processes such as glycolysis. For this, we adapt the characterization of the biochemical system to the needs of classical engineering systems theory: we combine online mass spectrometry and continuous system operation to apply standard system theory input functions and to use the detailed dynamic system responses to parameterize a model of sufficient quality for forward design...
2016: Nature Communications
https://www.readbyqxmd.com/read/27655524/cultivated-sub-populations-of-soil-microbiomes-retain-early-flowering-plant-trait
#7
Kevin Panke-Buisse, Stacey Lee, Jenny Kao-Kniffin
The collection of microorganisms found in the root zone of soil, termed the rhizosphere microbiome, has been shown to impact plant growth and development. Here, we tease apart the function of the cultivable portion of the microbiome from the whole microbiome in retaining plant traits modified through artificial selection on flowering time. Specifically, the whole microbiome associated with earlier flowering time of Arabidopsis thaliana was cultivated on four types of solid media to create cultivated fractions of the microbiome...
September 21, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27587179/note-electron-energy-spectroscopy-mapping-of-surface-with-scanning-tunneling-microscope
#8
Meng Li, Chunkai Xu, Panke Zhang, Zhean Li, Xiangjun Chen
We report a novel scanning probe electron energy spectrometer (SPEES) which combines a double toroidal analyzer with a scanning tunneling microscope to achieve both topography imaging and electron energy spectroscopy mapping of surface in situ. The spatial resolution of spectroscopy mapping is determined to be better than 0.7 ± 0.2 μm at a tip sample distance of 7 μm. Meanwhile, the size of the field emission electron beam spot on the surface is also measured, and is about 3.6 ± 0.8 μm in diameter. This unambiguously demonstrates that the spatial resolution of SPEES technique can be much better than the size of the incident electron beam...
August 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27586348/periplasmic-screening-for-artificial-metalloenzymes
#9
M Jeschek, S Panke, T R Ward
Artificial metalloenzymes represent an attractive means of combining state-of-the-art transition metal catalysis with the benefits of natural enzymes. Despite the tremendous recent progress in this field, current efforts toward the directed evolution of these hybrid biocatalysts mainly rely on the laborious, individual purification of protein variants rendering the throughput, and hence the outcome of these campaigns feeble. We have recently developed a screening platform for the directed evolution of artificial metalloenzymes based on the streptavidin-biotin technology in the periplasm of the Gram-negative bacterium Escherichia coli...
2016: Methods in Enzymology
https://www.readbyqxmd.com/read/27578890/tetramabs-simultaneous-targeting-of-four-oncogenic-receptor-tyrosine-kinases-for-tumor-growth-inhibition-in-heterogeneous-tumor-cell-populations
#10
Raffaella Castoldi, Jürgen Schanzer, Christian Panke, Ute Jucknischke, Natalie J Neubert, Rebecca Croasdale, Werner Scheuer, Johannes Auer, Christian Klein, Gerhard Niederfellner, Sebastian Kobold, Claudio Sustmann
Monoclonal antibody-based targeted tumor therapy has greatly improved treatment options for patients. Antibodies against oncogenic receptor tyrosine kinases (RTKs), especially the ErbB receptor family, are prominent examples. However, long-term efficacy of such antibodies is limited by resistance mechanisms. Tumor evasion by a priori or acquired activation of other kinases is often causative for this phenomenon. These findings led to an increasing number of combination approaches either within a protein family, e...
October 2016: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/27571282/directed-evolution-of-artificial-metalloenzymes-for-in-vivo-metathesis
#11
Markus Jeschek, Raphael Reuter, Tillmann Heinisch, Christian Trindler, Juliane Klehr, Sven Panke, Thomas R Ward
The field of biocatalysis has advanced from harnessing natural enzymes to using directed evolution to obtain new biocatalysts with tailor-made functions. Several tools have recently been developed to expand the natural enzymatic repertoire with abiotic reactions. For example, artificial metalloenzymes, which combine the versatile reaction scope of transition metals with the beneficial catalytic features of enzymes, offer an attractive means to engineer new reactions. Three complementary strategies exist: repurposing natural metalloenzymes for abiotic transformations; in silico metalloenzyme (re-)design; and incorporation of abiotic cofactors into proteins...
August 29, 2016: Nature
https://www.readbyqxmd.com/read/27444433/exploiting-racemases
#12
REVIEW
Christian Femmer, Matthias Bechtold, Tania Michelle Roberts, Sven Panke
Chiral resolutions of racemic mixtures are limited to a theoretical yield of 50 %. This yield can be doubled by integration of a step-wise or continuous racemization of the non-desired enantiomer. Many of the different routes along which the racemization step can be conducted require harsh treatments and are therefore often incompatible with the highly functionalized state of many compounds relevant for the life science industries. Employing enzymatic catalysis for racemization can therefore be highly beneficial...
September 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27363372/compartmentalization-a-prerequisite-for-maintaining-and-changing-an-identity
#13
Philipp Rottmann, Thomas Ward, Sven Panke
The chemical manipulation of DNA is much more convenient than the manipulation of the bioproducts, such as enzymes, that it encodes. The optimization of bioproducts requires cycles of diversification of DNA followed by read-out of the information into the bioproduct. Maintaining the link between the information - the genotype - and the properties of the bioproduct - the phenotype - through some form of compartmentalization is therefore an essential aspect in directed evolution. While the ideal compartment is a biological cell, many projects involving more radical changes in the bioproduct, such as the introduction of novel cofactors, may not be suitable for expression of the information in cells, and alternative in vitro methods have to be applied...
2016: Chimia
https://www.readbyqxmd.com/read/27324986/identification-and-characterisation-of-a-ph-stable-gfp
#14
Tania Michelle Roberts, Fabian Rudolf, Andreas Meyer, Rene Pellaux, Ellis Whitehead, Sven Panke, Martin Held
Green fluorescent proteins (GFPs) are invaluable tools for modern cell biology. Even though many properties of GFP have been successfully engineered, a GFP retaining brightness at low pH has not emerged. This limits the use of GFP in quantitative studies performed in fluctuating or acidic conditions. We report the engineering and characterisation of tandem dimer GFP (pH-tdGFP), a bright and stable GFP that can be efficiently excited and maintains its fluorescence properties in acidic conditions. Therefore, pH-tdGFP could act as a quantitative marker for cellular processes that occur at low pH, such as endocytosis, autophagy or starvation...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27322068/a-family-of-metal-dependent-phosphatases-implicated-in-metabolite-damage-control
#15
Lili Huang, Anna Khusnutdinova, Boguslaw Nocek, Greg Brown, Xiaohui Xu, Hong Cui, Pierre Petit, Robert Flick, Rémi Zallot, Kelly Balmant, Michael J Ziemak, John Shanklin, Valérie de Crécy-Lagard, Oliver Fiehn, Jesse F Gregory, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin, Andrew D Hanson
DUF89 family proteins occur widely in both prokaryotes and eukaryotes, but their functions are unknown. Here we define three DUF89 subfamilies (I, II, and III), with subfamily II being split into stand-alone proteins and proteins fused to pantothenate kinase (PanK). We demonstrated that DUF89 proteins have metal-dependent phosphatase activity against reactive phosphoesters or their damaged forms, notably sugar phosphates (subfamilies II and III), phosphopantetheine and its S-sulfonate or sulfonate (subfamily II-PanK fusions), and nucleotides (subfamily I)...
August 2016: Nature Chemical Biology
https://www.readbyqxmd.com/read/27208865/discontinuation-of-a-left-ventricular-assist-device-in-the-home-hospice-setting
#16
Joan T Panke, George Ruiz, Tonya Elliott, Daniel J Pattenden, Evan G DeRenzo, Ellen E Rollins, Hunter Groninger
No abstract text is available yet for this article.
August 2016: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/27161115/regulation-of-coenzyme-a-biosynthesis-in-the-hyperthermophilic-bacterium-thermotoga-maritima
#17
Takahiro Shimosaka, Hiroya Tomita, Haruyuki Atomi
UNLABELLED: Regulation of coenzyme A (CoA) biosynthesis in bacteria and eukaryotes occurs through feedback inhibition targeting type I and type II pantothenate kinase (PanK), respectively. In contrast, the activity of type III PanK is not affected by CoA. As the hyperthermophilic bacterium Thermotoga maritima harbors only a single type III PanK (Tm-PanK), here we examined the mechanisms that regulate CoA biosynthesis in this organism. We first examined the enzyme responsible for the ketopantoate reductase (KPR) reaction, which is the target of feedback inhibition in archaea...
July 15, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27137922/-not-available
#18
Thomas G Wendt, G Gademann, C Pambor, I Grießbach, H von Specht, T Martin, D Baltas, R Kurek, S Röddiger, U W Tunn, N Zamboglou, H T Eich, S Staar, A Gossmann, K Hansemann, R Semrau, R Skripnitchenko, V Diehl, R-P Müller, S Sehlen, N Willich, U Rühl, P Lukas, E Dühmke, K Engel, E Tabbert, M Bolck, S Knaack, H Annweiler, R Krempien, H Hoppe, W Harms, S Daeuber, O Schorr, M Treiber, J Debus, M Alber, F Paulsen, M Birkner, A Bakai, C Belka, W Budach, K-H Grosser, R Kramer, B Kober, M Reinert, P Schneider, A Hertel, H Feldmann, P Csere, C Hoinkis, G Rothe, P Zahn, H Alheit, S X Cavanaugh, P Kupelian, C Reddy, B Pollock, M Fuss, S Roeddiger, T Dannenberg, B Rogge, D Drechsler, T Herrmann, W Alberti, R Schwarz, M Graefen, A Krüll, V Rudat, H Huland, C Fehr, C Baum, S Glocker, F Nüsslin, T Heil, H Lemnitzer, M Knips, O Baumgart, W Thiem, K-H Kloetzer, L Hoffmann, B Neu, B Hültenschmidt, M-L Sautter-Bihl, O Micke, M H Seegenschmiedt, D Köppen, G Klautke, R Fietkau, J Schultze, G Schlichting, H Koltze, B Kimmig, M Glatzel, D Fröhlich, S Bäsecke, A Krauß, D Strauß, K-J Buth, R Böhme, W Oehler, D Bottke, U Keilholz, K Heufelder, T Wiegel, W Hinkelbein, C Rödel, T Papadopoulos, M Munnes, R Wirtz, R Sauer, F Rödel, D Lubgan, L Distel, G G Grabenbauer, A Sak, G Stüben, C Pöttgen, S Grehl, M Stuschke, K Müller, C Pfaffendorf, A Mayerhofer, F M Köhn, J Ring, D van Beuningen, V Meineke, S Neubauer, U Keller, M Wittlinger, D Riesenbeck, B Greve, R Exeler, M Ibrahim, C Liebscher, E Severin, O Ott, R Pötter, J Hammer, G Hildebrandt, M W Beckmann, V Strnad, F Fehlauer, S Tribius, A Bajrovic, U Höller, D Rades, A Warszawski, R Baumann, B Madry-Gevecke, J H Karstens, C Grehn, F Hensley, C Berns, M Wannenmacher, S Semrau, T Reimer, B Gerber, P Ketterer, E Koepcke, G Hänsgen, H G Strauß, J Dunst, J Füller, S Kalb, T Wendt, H D Weitmann, C Waldhäusl, T-H Knocke, U Lamprecht, J Classen, T W Kaulich, B Aydeniz, M Bamberg, T Wiezorek, N Banz, H Salz, M Scheithauer, M Schwedas, J Lutterbach, S Bartelt, H Frommhold, J Lambert, D Hornung, S Swiderski, M Walke, A Siefert, B Pöllinger, K Krimmel, M Schaffer, O Koelbl, K Bratengeier, D Vordermark, M Flentje, B Hero, F Berthold, S E Combs, S Gutwein, D Schulz-Ertner, M van Kampen, C Thilmann, M Kocher, S Kunze, S Schild, K Ikezaki, B Müller, R Sieber, C Weiß, I Wolf, F Wenz, K-J Weber, J Schäfer, A Engling, S Laufs, M R Veldwijk, D Milanovic, K Fleckenstein, W Zeller, S Fruehauf, C Herskind, M Weinmann, V Jendrossek, C Rübe, S Appold, S Kusche, T Hölscher, K Brüchner, P Geyer, M Baumann, R Kumpf, F Zimmermann, S Schill, H Geinitz, C Nieder, B Jeremic, M Molls, S Liesenfeld, H Petrat, S Hesselmann, U Schäfer, F Bruns, E Horst, R Wilkowski, G Assmann, A Nolte, J Diebold, U Löhrs, P Fritz, K Hans-Jürgen, W Mühlnickel, P Bach, B Wahlers, H-J Kraus, J Wulf, U Hädinger, K Baier, T Krieger, G Müller, H Hof, K Herfarth, T Brunner, S M Hahn, F S Schreiber, A K Rustgi, W G McKenna, E J Bernhard, M Guckenberger, K Meyer, J Willner, M Schmidt, M Kolb, M Li, P Gong, A Abdollahi, T Trinh, P E Huber, H Christiansen, B Saile, K Neubauer-Saile, S Tippelt, M Rave-Fränk, R M Hermann, J Dudas, C F Hess, H Schmidberger, G Ramadori, N Andratschke, R Price, K-K Ang, S Schwarz, U Kulka, M Busch, L Schlenger, J Bohsung, I Eichwurzel, G Matnjani, D Sandrock, M Richter, R Wurm, V Budach, A Feussner, J Gellermann, A Jordan, R Scholz, U Gneveckow, K Maier-Hauff, R Ullrich, P Wust, R Felix, N Waldöfner, M Seebass, H-J Ochel, A Dani, A Varkonyi, M Osvath, A Szasz, P M Messer, N M Blumstein, H-W Gottfried, E Schneider, S N Reske, E M Röttinger, A-L Grosu, M Franz, S Stärk, W Weber, M Heintz, F Indenkämpen, T Beyer, W Lübcke, S Levegrün, J Hayen, N Czech, B Mbarek, R Köster, H Thurmann, M Todorovic, A Schuchert, T Meinertz, T Münzel, H Grundtke, B Hornig, T Hehr, C Dilcher, R C Chan, G S Mintz, J-I Kotani, V M Shah, D A Canos, N J Weissman, R Waksman, R Wolfram, B Bürger, M Schrappe, B Timmermann, A Lomax, G Goitein, A Schuck, A Mattke, C Int-Veen, I Brecht, S Bernhard, J Treuner, E Koscielniak, F Heinze, M Kuhlen, I von Schorlemer, S Ahrens, A Hunold, S Könemann, W Winkelmann, H Jürgens, J Gerstein, B Polivka, K-W Sykora, M Bremer, R Thamm, C Höpfner, H Gumprecht, R Jäger, M A Leonardi, A M Frank, A E Trappe, C B Lumenta, E Östreicher, K Pinsker, A Müller, C Fauser, W Arnold, M Henzel, M W Groß, R Engenhart-Cabillic, P Schüller, S Palkovic, J Schröder, H Wassmann, A Block, R Bauer, F-W Keffel, B Theophil, L Wisser, M Rogger, M Niewald, V van Lengen, K Mathias, G Welzel, M Bohrer, S Steinvorth, C Schleußner, K Leppert, B Röhrig, B Strauß, B van Oorschot, N Köhler, R Anselm, A Winzer, T Schneider, U Koch, K Schönekaes, R Mücke, J Büntzel, K Kisters, C Scholz, M Keller, C Winkler, N Prause, R Busch, S Roth, I Haas, R Willers, S Schultze-Mosgau, J Wiltfang, P Kessler, F W Neukam, B Röper, N Nüse, F Auer, W Melzner, M Geiger, M Lotter, T Kuhnt, A C Müller, N Jirsak, C Gernhardt, H-G Schaller, B Al-Nawas, M O Klein, C Ludwig, J Körholz, K A Grötz, K Huppers, M Kunkel, T Olschewski, K Bajor, B Lang, E Lang, U Kraus-Tiefenbacher, R Hofheinz, B von Gerstenberg-Helldorf, F Willeke, A Hochhaus, M Roebel, S Oertel, S Riedl, M Buechler, T Foitzik, K Ludwig, E Klar, A Meyer, J Meier Zu Eissen, D Schwab, T Meyer, S Höcht, A Siegmann, F Sieker, S Pigorsch, B Milicic, L Acimovic, S Milisavljevic, G Radosavljevic-Asic, N Presselt, R P Baum, D Treutler, R Bonnet, M Schmücking, D Sammour, T Fink, J Ficker, O Pradier, K Lederer, E Weiss, A Hille, S Welz, S Sepe, G Friedel, W Spengler, E Susanne, O Kölbl, W Hoffmann, B Wörmann, A Günther, M Becker-Schiebe, J Güttler, C Schul, M Nitsche, M K Körner, R Oppenkowski, F Guntrum, L Malaimare, M Raub, C Schöfl, T Averbeck, I Hacker, H Blank, C Böhme, D Imhoff, K Eberlein, S Weidauer, H D Böttcher, L Edler, M Tatagiba, H Molina, C Ostertag, S Milker-Zabel, A Zabel, W Schlegel, A Hartmann, I Wildfang, G Kleinert, K Hamm, W Reuschel, R Wehrmann, P Kneschaurek, M W Münter, A Nikoghosyan, B Didinger, S Nill, B Rhein, D Küstner, U Schalldach, D Eßer, H Göbel, H Wördehoff, S Pachmann, H Hollenhorst, K Dederer, C Evers, J Lamprecht, A Dastbaz, B Schick, J Fleckenstein, P K Plinkert, Chr Rübe, T Merz, B Sommer, A Mencl, V Ghilescu, S Astner, A Martin, F Momm, N J Volegova-Neher, J Schulte-Mönting, R Guttenberger, A Buchali, E Blank, D Sidow, W Huhnt, T Gorbatov, A Heinecke, G Beckmann, A-M Bentia, H Schmitz, U Spahn, V Heyl, P-J Prott, R Galalae, R Schneider, C Voith, A Scheda, B Hermann, L Bauer, F Melchert, N Kröger, A Grüneisen, F Jänicke, A Zander, I Zuna, I Schlöcker, K Wagner, E John, T Dörk, G Lochhas, M Houf, D Lorenz, K-H Link, F-J Prott, M Thoma, R Schauer, V Heinemann, M Romano, M Reiner, A Quanz, U Oppitz, R Bahrehmand, M Tine, A Naszaly, P Patonay, Á Mayer, K Markert, S-K Mai, F Lohr, B Dobler, M Pinkawa, K Fischedick, P Treusacher, D Cengiz, R Mager, H Borchers, G Jakse, M J Eble, B Asadpour, B Krenkel, R Holy, Y Kaplan, T Block, H Czempiel, U Haverkamp, B Prümer, T Christian, P Benkel, C Weber, S Gruber, P Reimann, J Blumberg, K Krause, A-R Fischedick, K Kaube, K Steckler, B Henzel, N Licht, T Loch, A Krystek, A Lilienthal, H Alfia, J Claßen, P Spillner, B Knutzen, R Souchon, I Schulz, K Grüschow, U Küchenmeister, H Vogel, D Wolff, U Ramm, J Licner, F Rudolf, J Moog, C G Rahl, S Mose, H Vorwerk, E Weiß, A Engert, I Seufert, F Schwab, J Dahlke, T Zabelina, W Krüger, H Kabisch, V Platz, J Wolf, B Pfistner, B Stieltjes, T Wilhelm, M Schmuecking, K Junker, D Treutier, C P Schneider, J Leonhardi, A Niesen, K Hoeffken, A Schmidt, K-M Mueller, I Schmid, K Lehmann, C G Blumstein, R Kreienberg, L Freudenberg, H Kühl, M Stahl, B Elo, P Erichsen, H Stattaus, T Welzel, U Mende, S Heiland, B J Salter, R Schmid, D Stratakis, R M Huber, J Haferanke, N Zöller, M Henke, J Lorenzen, B Grzyska, A Kuhlmey, G Adam, V Hamelmann, T Bölling, H Job, J E Panke, P Feyer, S Püttmann, B Siekmeyer, H Jung, B Gagel, U Militz, M Piroth, A Schmachtenberg, T Hoelscher, C Verfaillie, B Kaminski, E Lücke, H Mörtel, W Eyrich, M Fritsch, J-C Georgi, C Plathow, H Zieher, F Kiessling, P Peschke, H-U Kauczor, J Licher, O Schneider, R Henschler, C Seidel, A Kolkmeyer, T P Nguyen, K Janke, M Michaelis, M Bischof, C Stoffregen, K Lipson, K Weber, V Ehemann, D Jürgen, P Achanta, K Thompson, J L Martinez, T Körschgen, R Pakala, E Pinnow, D Hellinga, F O'Tio, A Katzer, A Kaffer, A Kuechler, S Steinkirchner, N Dettmar, N Cordes, S Frick, M Kappler, H Taubert, F Bartel, H Schmidt, M Bache, S Frühauf, T Wenk, K Litzenberger, M Erren, F van Valen, L Liu, K Yang, J Palm, M Püsken, M Behe, T M Behr, P Marini, A Johne, U Claussen, T Liehr, V Steil, C Moustakis, I Griessbach, A Oettel, C Schaal, M Reinhold, G Strasssmann, I Braun, P Vacha, D Richter, T Osterham, P Wolf, G Guenther, M Miemietz, E A Lazaridis, B Forthuber, M Sure, J Klein, H Saleske, T Riedel, P Hirnle, G Horstmann, H Schoepgens, A Van Eck, O Bundschuh, A Van Oosterhut, K Xydis, K Theodorou, C Kappas, J Zurheide, N Fridtjof, U Ganswindt, N Weidner, M Buchgeister, B Weigel, S B Müller, M Glashörster, C Weining, B Hentschel, O A Sauer, W Kleen, J Beck, D Lehmann, S Ley, C Fink, M Puderbach, W Hosch, A Schmähl, K Jung, A Stoßberg, E Rolf, M Damrau, D Oetzel, U Maurer, G Maurer, K Lang, J Zumbe, D Hahm, H Fees, B Robrandt, U Melcher, M Niemeyer, A Mondry, V Kanellopoulos-Niemeyer, H Karle, D Jacob-Heutmann, C Born, W Mohr, J Kutzner, M Thelen, M Schiebe, U Pinkert, L Piasswilm, F Pohl, S Garbe, K Wolf, Y Nour, P Barwig, D Trog, C Schäfer, M Herbst, B Dietl, M Cartes, F Schroeder, G Sigingan-Tek, R Feierabend, S Theden, A Schlieck, M Gotthardt, U Glowalla, S Kremp, O Hamid, N Riefenstahl, B Michaelis, G Schaal, E Liebermeister, U Niewöhner-Desbordes, M Kowalski, N Franz, W Stahl, C Baumbach, J Thale, W Wagner, B Justus, A L Huston, R Seaborn, P Rai, S-W Rha, G Sakas, S Wesarg, P Zogal, B Schwald, H Seibert, R Berndt-Skorka, G Seifert, K Schoenekaes, C Bilecen, W Ito, G Matschuck, D Isik
No abstract text is available yet for this article.
June 2004: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://www.readbyqxmd.com/read/27029461/rationally-reduced-libraries-for-combinatorial-pathway-optimization-minimizing-experimental-effort
#19
Markus Jeschek, Daniel Gerngross, Sven Panke
Rational flux design in metabolic engineering approaches remains difficult since important pathway information is frequently not available. Therefore empirical methods are applied that randomly change absolute and relative pathway enzyme levels and subsequently screen for variants with improved performance. However, screening is often limited on the analytical side, generating a strong incentive to construct small but smart libraries. Here we introduce RedLibs (Reduced Libraries), an algorithm that allows for the rational design of smart combinatorial libraries for pathway optimization thereby minimizing the use of experimental resources...
March 31, 2016: Nature Communications
https://www.readbyqxmd.com/read/26993838/venous-duplex-and-pathologic-differences-in-thrombus-characteristics-between-de-novo-deep-vein-thrombi-and-endovenous-heat-induced-thrombi
#20
Brian J Santin, Joanne M Lohr, Thomas W Panke, Patrick M Neville, Melissa M Felinski, Brian A Kuhn, Matthew H Recht, Patrick E Muck
OBJECTIVE: Superficial venous reflux disease has been treated with endovenous ablation techniques for more than 15 years. Thrombi discovered in the postoperative period are referred to as endovenous heat-induced thrombi (EHIT). In spite of the few studies of the ultrasound differentiation between EHIT and deep vein thrombi (DVT), there remains a paucity of literature regarding the evaluation of ultrasound examination and pathologic differentiation. METHODS: Six Yorkshire cross swine underwent femoral vein thrombosis by suture ligation or endovenous radiofrequency ablation...
April 2015: Journal of Vascular Surgery. Venous and Lymphatic Disorders
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