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Tissue engineer vagina

Rui Liang, Katrina Knight, William Barone, Robert W Powers, Alexis Nolfi, Stacy Palcsey, Steven Abramowitch, Pamela A Moalli
BACKGROUND: The use of wide pore lightweight polypropylene mesh to improve anatomical outcomes in the surgical repair of prolapse has been hampered by mesh complications. One of the prototype prolapse meshes has been found to negatively impact the vagina by inducing a decrease in smooth muscle volume and contractility and the degradation of key structural proteins (collagen and elastin), resulting in vaginal degeneration. Recently, bioscaffolds derived from extracellular matrix have been used to mediate tissue regeneration and have been widely adopted in tissue engineering applications...
September 8, 2016: American Journal of Obstetrics and Gynecology
Jing Ding, Mou Deng, Xiao-Chen Song, Chun Chen, Kui-Lin Lai, Guo-Shuai Wang, Yu-Yu Yuan, Tao Xu, Lan Zhu
BACKGROUND: Implantation of nonabsorbable polypropylene (PP) mesh in the vagina is the main surgical treatment for pelvic organ prolapse (POP); however, clinical outcomes remain controversial and far from satisfactory. In particular, reducing the exposure or erosion of vaginal implants to obtain improved functional reconstruction is challenging. There is an urgent need for the development of new materials and/or products for POP treatment. A nanofibrous biomimetic mesh was recently developed to address this issue...
August 2016: Journal of the Mechanical Behavior of Biomedical Materials
Yachai Li, Xianghua Huang, Mingle Zhang, Yanan Li, Yexing Chen, Jingfei Jia
OBJECTIVE: To explore the biocompatibility of the poly-lactide-co-glycolide (PLGA)/collagen type I scaffold with rat vaginal epithelial cells, and the feasibility of using PLGA/collagen type I as scaffold to reconstruct vagina by the tissue engineering. METHODS: PLGA/collagen type I scaffold was prepared with PLGA covered polylysine and collagen type I. The vaginal epithelial cells of Sprague Dawley rat of 10-12 weeks old were cultured by enzyme digestion method...
September 2015: Chinese Journal of Reparative and Reconstructive Surgery
Marla J Keller, Pedro M Mesquita, Mark A Marzinke, Ryan Teller, Lilia Espinoza, Jessica M Atrio, Yungtai Lo, Bruce Frank, Sujatha Srinivasan, David N Fredricks, Lorna Rabe, Peter L Anderson, Craig W Hendrix, Patrick F Kiser, Betsy C Herold
BACKGROUND: Tenofovir disoproxil fumarate (TDF), a prodrug of tenofovir (TFV), may be ideal for topical HIV preexposure prophylaxis because it has higher tissue and cell permeability than TFV; is not adversely impacted by seminal proteins; and its active metabolite, TFV-diphosphate (TFV-DP), has a long intracellular half-life. We engineered a TDF eluting polyurethane reservoir intravaginal ring (IVR) to provide near constant mucosal antiretroviral concentrations. METHODS: A first-in-human randomized placebo-controlled trial was conducted to assess the safety and pharmacokinetics of the TDF IVR in healthy, sexually abstinent women (15 TDF and 15 placebo)...
March 13, 2016: AIDS
Marco Antonio Ávila-Vergara, Damián Alfredo León-Álvarez, Miriam Nayeli López-Villegas, Samantha Melissa Quintero-Medrano, Gladys Fabiola Angulo-Bueno, Felipe Vadillo-Ortega
Agenesia of the Müllerian ducts is a low-frequency congenital disease but with devastating effects on women's reproductive health. In this paper we present two cases of women affected by Mayer-Rokitansky-Küster-Hauser syndrome (MRKH). First case was a 17-year-old woman with aplasia of the upper vagina and absence of uterus. No other defects were found and was classified as type 1-MRKH. Second case was 18-year-old woman with absence of uterus, escoliosis and polycystic ovary syndrome, classified as type II-MRKH...
March 2015: Ginecología y Obstetricia de México
Yanan Li, Fangfang Liu, Zhiqiang Zhang, Mingle Zhang, Shanjin Cao, Yachai Li, Lin Zhang, Xianghua Huang, Yanfang Xu
Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined whether MSCs could be differentiated to vaginal epithelial cells (VECs) upon co-culturing with VECs. We also examined whether Wnt/β-catenin signaling pathway is implicated in such differentiation...
November 2015: Cell Biology International
Anita Gupta, Andrea Bischoff, Alberto Peña, Laura A Runck, Géraldine Guasch
The anorectal and urogenital systems arise from a common embryonic structure termed cloaca. Subsequent development leads to the division/septation of the cloaca into the urethra, urinary bladder, vagina, anal canal, and rectum. Defective cloacal development and the resulting anorectal and urogenital malformations are some of the most severe congenital anomalies encountered in children. In the most severe form in females, the rectum, vagina, and urethra fail to develop separately and drain via a single common channel known as a cloaca into the perineum...
November 2014: Pediatric Surgery International
David S Bombard, Shaker A Mousa
Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome is a disorder in women that presents as Mullerian agenesis. These patients present internal genitalia abnormalities that include the absence of a uterus and the upper two-thirds of the vagina. In this review, current diagnostic methods, accompanying complications (congenital and psychological) and non-surgical and surgical treatments are summarized. Ultrasound and MRI have been the most documented options in MRKH syndrome diagnosis. Many women with MRKH syndrome have renal, skeletal, hearing or cardiac congenital anomalies and increased levels of psychological distress...
September 2014: Gynecological Endocrinology
Atlántida M Raya-Rivera, Diego Esquiliano, Reyna Fierro-Pastrana, Esther López-Bayghen, Pedro Valencia, Ricardo Ordorica-Flores, Shay Soker, James J Yoo, Anthony Atala
BACKGROUND: Several disorders might require vaginal reconstruction, such as congenital abnormalities, injury, or cancer. Reconstructive techniques for which non-vaginal tissue is used can be associated with complications. We assessed the use of engineered vaginal organs in four patients with vaginal aplasia caused by Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS). METHODS: We invited to participate four consecutive patients who presented over a 3-year period with congenital vaginal aplasia due to MRKHS...
July 26, 2014: Lancet
Martin A Birchall, Alexander M Seifalian
No abstract text is available yet for this article.
July 26, 2014: Lancet
Daniela Ulrich, Sharon Lee Edwards, Kai Su, Ker Sin Tan, Jacinta F White, John A M Ramshaw, Camden Lo, Anna Rosamilia, Jerome A Werkmeister, Caroline E Gargett
BACKGROUND: Pelvic organ prolapse (POP) is defined as the descent of one or more of the pelvic structures into the vagina and includes uterine, vaginal vault, and anterior or posterior vaginal wall prolapse. The treatment of POP may include implantation of a synthetic mesh. However, the long-term benefit of mesh surgery is controversial due to complications such as mesh exposure or pain. The aim of this study was to use a tissue engineering (TE) approach to assess the in vivo biological and biomechanical behavior of a new gelatin/polyamide mesh, seeded with a novel source of mesenchymal stem cells in a subcutaneous rat model of wound repair...
February 2014: Tissue Engineering. Part A
Lan Zhu, Huimei Zhou, Zhixing Sun, Wenjia Lou, Jinghe Lang
INTRODUCTION: Recent years have seen continuous reports about the successful reconstruction of numerous organs with the application of tissue-engineering techniques. Thus, we assess the outcomes for vagina reconstruction using tissue-engineered biological material, which we suggested previously as an ideal graft for vaginoplasty. AIM: To evaluate the anatomic and sexual outcomes in patients undergoing vaginoplasty using tissue-engineered biomaterial mesh. METHODS: This prospective study included 53 patients with Mayer-Rokitansky-Küster-Hauser syndrome admitted to our hospital...
June 2013: Journal of Sexual Medicine
Anthony Atala, F Kurtis Kasper, Antonios G Mikos
Tissue engineering has emerged at the intersection of numerous disciplines to meet a global clinical need for technologies to promote the regeneration of functional living tissues and organs. The complexity of many tissues and organs, coupled with confounding factors that may be associated with the injury or disease underlying the need for repair, is a challenge to traditional engineering approaches. Biomaterials, cells, and other factors are needed to design these constructs, but not all tissues are created equal...
November 14, 2012: Science Translational Medicine
M Boennelycke, S Gras, G Lose
INTRODUCTION AND HYPOTHESIS: Cell-based tissue engineering strategies could potentially provide attractive alternatives to surgical reconstruction of native tissue or the use of surgical implants in treating pelvic organ prolapse (POP). METHODS: Based on a search in PubMed, this review focuses on candidate cell types, scaffolds, and trophic factors used in studies examining cell-based tissue engineering strategies to treat POP, stress urinary incontinence (SUI), and the closely related field of hernias...
May 2013: International Urogynecology Journal
Anant Agrawal, Dianne E Godar
We developed a triple-labeling immunofluorescence technique that simultaneously identifies total DNA (DAPI), DNA damage (antibodies), and dead cells [terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells] and a method that semiquantifies DNA damage in paraffin-embedded tissues. Using this technique in combination with our analysis method, scientists can now simultaneously detect and compare the relative amounts of DNA damage of almost any kind (except single-strand and double-strand breaks), using indirect fluorescent antibody labeling, in both normal and dying cells of different tissues...
July 2012: Applied Immunohistochemistry & Molecular Morphology: AIMM
Antonio Peramo, Cynthia L Marcelo, Stephen E Feinberg
We report for the first time the fabrication of a three-dimensional tissue structure containing, in a continuous layer, the morphological features of a lip: epidermal skin, vermillion, and oral mucosa. This tissue engineered muco-cutaneous (M/C) equivalent was manufactured using human oral and skin keratinocytes grown on an acellular, nonimmunogenic dermal equivalent (AlloDerm(®)) to produce a tissue equivalent with similar anatomic and handling properties as native human lips. Confirmation of the structural composition of the construct was performed using routine histology and immunohistochemistry by identification of epithelial markers that are differentially expressed in separate anatomic areas of the lips...
April 2012: Tissue Engineering. Part C, Methods
Gertrude-Emilia Costin, Hans A Raabe, Robert Priston, Eric Evans, Roger D Curren
Mucosal surfaces, such as the vaginal epithelium, are natural barriers to infection that are constantly exposed to bacteria and viruses, and are therefore potential sites of entry for numerous pathogens. The vaginal epithelium can be damaged mechanically, e.g. by the incorrect use of objects such as tampons, and by chemicals that are irritating or corrosive. Consequently, this can lead to an increase in susceptibility to further damage or infection. Pharmaceutical, cosmetic and personal care products that are specifically formulated for application onto human external mucosae can occasionally induce undesirable local or systemic side-effects...
September 2011: Alternatives to Laboratory Animals: ATLA
Alamelu Mahalingam, Julie I Jay, Kristofer Langheinrich, Shetha Shukair, Mike D McRaven, Lisa C Rohan, Betsy C Herold, Thomas J Hope, Patrick F Kiser
In conjunction with the routine role of delivering the active ingredient, carefully designed drug delivery vehicles can also provide ancillary functions that augment the overall efficacy of the system. Inspired by the ability of the cervicovaginal mucus to impede the movement of HIV virions at acidic pH, we have engineered a pH-responsive synthetic polymer that shows improved barrier properties over the naturally occurring cervicovaginal mucus by inhibiting viral transport at both acidic and neutral pH. The pH-responsive synthetic mucin-like polymer is constructed with phenylboronic acid (PBA) and salicylhydroxamic acid (SHA), each individually copolymerized with a 2-hydroxypropyl methacrylamide (pHPMA) polymer backbone...
November 2011: Biomaterials
Mingle Zhang, Yachai Li, Xianghua Huang
OBJECTIVE: To summarize the research and development of vaginal reconstruction with tissue engineering technology. METHODS: The recent literature concerning vaginal reconstruction with tissue engineering technology at home and abroad was extensively reviewed and the research and development were summarized. RESULTS: Tissue engineering provides an ideal material as the inner tissue in vaginoplasty. The reconstructed tissue closely resembles native vaginal tissue in the cellular organization and physical properties...
July 2011: Chinese Journal of Reparative and Reconstructive Surgery
Jianing Meng, Timothy F Sturgis, Bi-Botti C Youan
The objective of this study was to engineer a model anti-HIV microbicide (tenofovir) loaded chitosan based nanoparticles (NPs). Box-Behnken design allowed to assess the influence of formulation variables on the size of NPs and drug encapsulation efficiency (EE%) that were analyzed by dynamic light scattering and UV spectroscopy, respectively. The effect of the NPs on vaginal epithelial cells and Lactobacillus crispatus viability and their mucoadhesion to porcine vaginal tissue were assessed by cytotoxicity assays and fluorimetry, respectively...
September 18, 2011: European Journal of Pharmaceutical Sciences
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