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https://www.readbyqxmd.com/read/27852634/calibration-and-evaluation-of-quantitative-antibody-titers-for-measles-virus-using-the-bioplex-2200
#1
Todd F Hatchette, Heidi Scholz, Shelly Bolotin, Natasha S Crowcroft, Colleen Jackson, Elizabeth McLachlan, Alberto Severini
The BioPlex 2200 (Bio-Rad Laboratories, Hercules, Ca) is a rapid, automated platform, which can screen large numbers of specimens for antibodies to measles, mumps, rubella and varicella. Although approved for producing qualitative results, in this study we have validated the test (off-label) to allow reporting of quantitative results. To do this, we used the 3(rd) anti-measles WHO standard to generate a calibration curve that allowed relative fluorescence intensity to be translated into quantitative antibody titer (Antibody Units/mL (AU/mL))...
November 16, 2016: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/27830020/evaluation-of-a-research-use-only-luminex-based-assay-for-measurement-of-procalcitonin-in-serum-samples
#2
Charles Garrigan, Jennifer Han, Pam Tolomeo, Katherine J Johnson, Stephen R Master, Ebbing Lautenbach, Irving Nachamkin
Research use only (RUO) assays do not undergo a validation process similar to test kits used for clinical purposes. Several studies have suggested that RUO assays need to be validated prior to use in any research studies. We evaluated a research use only Luminex platform based assay for measuring serum procalcitonin levels (Bio-Plex Pro(TM) Human Acute Phase Multiplex Assay, Bio-Rad Laboratories, Hercules, CA) for comparability with an FDA cleared assay for procalcitonin (VIDAS B.R.A.H.M.S. PCT Assay, bioMérieux, Durham, NC)...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27816623/antimicrobial-photodynamic-therapy-using-indocyanine-green-and-near-infrared-diode-laser-in-reducing-entrerococcus-faecalis
#3
Charis Beltes, Hercules Sakkas, Nikolaos Economides, Chrissanthy Papadopoulou
BACKGROUND: The use of Antimicrobial Photodynamic Therapy (aPDT) has been suggested as an adjuvant method to eliminate facultative bacteria during root canal disinfection. The purpose of this preliminary in vitro study was to determine whether the light-activated antimicrobial agent, Indocyanine green (ICG), could be used as photosensitizer and kill Enterococcus faecalis strain under planktonic conditions when irradiated with near-infared (NIR) diode laser emitting in 810nm wavelength...
November 2, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27814264/the-influence-of-ph-and-chemical-composition-of-beverages-on-color-stability-of-a-nanofilled-composite-resin
#4
Hercules Bezerra Dias, Emanuelle Teixeira Carrera, Alessandra Nara de Souza Rastelli
Due to increasing consumer knowledge about the health benefits associated with anthocyanin-rich foods and beverages-such as acai and blueberry juices-the growing consumption of these products should be a source of concern for dentists. Since anthocyanins are colorants that can discolor teeth and restorations, the aim of this study was to investigate the staining effects of 2 anthocyanin-rich beverages on a nanofilled composite resin. Specimens were prepared, separated into 3 groups, and immersed in either artificial saliva or 1 of the 2 staining solutions (blueberry or acai juice) at 37°C for 7 days...
November 2016: General Dentistry
https://www.readbyqxmd.com/read/27811659/redescription-and-phylogenetic-analysis-of-the-type-species-of-the-giant-pill-millipede-genus-sphaeropoeus-brandt-1833-diplopoda-sphaerotheriida-zephroniidae
#5
Thomas Wesener
Sphaeropoeus hercules Brandt, 1833, the type species of one of the oldest genus names in Sphaerotheriida, is redescribed for the first time based on its fragmented type specimen. While damaged, the specimen shows characters clearly different from other, diverse and similar genera of the family Zephroniidae, such as Zephronia Gray, 1832 and Castanotherium Pocock, 1895. S. hercules has a record number of >180 apical cones on the antennae, more than any other millipede. The species, together with another species of the genus Castanotherium, C...
November 2, 2016: Zootaxa
https://www.readbyqxmd.com/read/27776288/hyaluronic-acid-on-collagen-membranes-an-experimental-study-in-rats
#6
Edson Costa E Silva, Sheyla Viana Omonte, Alessandro Gomides Veiga Martins, Hércules Henrique Onibene de Castro, Hayder Egg Gomes, Élton Gonçalves Zenóbio, Peterson Antônio Dutra de Oliveira, Martinho Campolina Rebello Horta, Paulo Eduardo Alencar Souza
OBJECTIVE: The aim of this study was to evaluate the effect of hyaluronic acid (HA) in the structure and degradation patterns of BioGide(®) and OsseoGuard™ collagen membranes. HA mediates inflammation and acts in cell migration, adhesion, and differentiation, benefitting tissue remodeling and vascularization. These are desirable effects in guided regeneration procedures, but it is still unknown whether HA alters the barrier properties of absorbable membranes. DESIGN: Bone defects were created in the calvaria of rats, which were treated with HA gel 1% (HA group) or simply filled with blood clot (control group), and covered with BioGide(®) or OsseoGuard™...
October 17, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27693249/exposure-of-cultured-fibroblasts-to-the-peptide-pr-11-for-the-identification-of-induced-proteome-alterations-and-discovery-of-novel-potential-ligands
#7
Gustavo Silveira Breguez, Leandro Xavier Neves, Karina Taciana Santos Silva, Lorran Miranda Andrade de Freitas, Gabriela de Oliveira Faria, Mauro César Isoldi, William Castro-Borges, Milton Hércules Guerra de Andrade
The PR-11 peptide corresponds to the N-terminal and active region of the endogenously synthesized PR-39 molecule, of porcine origin. It is known to possess various biological effects including antimicrobial properties, angiogenic and anti-inflammatory activities. Apart from its reported activity as a proteasome inhibitor, a more comprehensive understanding of its function, at the molecular level, is still lacking. In this study, we used a label-free shotgun strategy to evaluate the proteomic alterations caused by exposure of cultured fibroblasts to the peptide PR-11...
December 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27688085/differentiation-and-prognostic-markers-in-ampullary-cancer-role-of-p53-mdm2-cdx2-mucins-and-cytokeratins
#8
Iraklis Perysinakis, Emilia Minaidou, Dimitrios Mantas, George C Sotiropoulos, Vasileia Leontara, Hercules Tsipras, George N Zografos, Ilias Margaris, Gregory Kouraklis
INTRODUCTION: Subclassification of ampullary adenocarcinomas into intestinal and pancreatobiliary type has prognostic and therapeutic implications. Immunohistochemical staining against specific biomarkers has been proven to be a useful adjunct in determining the exact histotype. Furthermore the immunohistochemical profile is suggestive of the molecular pathogenic mechanisms through which the tumor evolved. The aim of this study was to correlate p53, MDM2, CK7, CK20, MUC1, MUC2 and CDX2 expression in ampullary adenocarcinomas with the type of differentiation and patients' survival...
November 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/27661063/parapatric-genetic-introgression-and-phenotypic-assimilation-testing-conditions-for-introgression-between-hercules-beetles-dynastes-dynastinae
#9
Jen-Pan Huang
The prevalence and consequences of genetic introgression between species have been intensively debated. I used Hercules beetles as examples to test for conditions that may be associated with the occurrence of introgression. RADseq data were used to reconstruct the species tree and history of introgression between Hercules beetles. Image data from museum specimens were used to investigate the phenotypic similarity of two adaptive traits between species from two distinct climatic realms (Nearctic vs. Neotropical)...
September 23, 2016: Molecular Ecology
https://www.readbyqxmd.com/read/27639320/long-term-clinical-outcomes-in-implantable-cardioverter-defibrillator-recipients-on-the-island-of-crete
#10
Emmanuel M Kanoupakis, John A Fanourgiakis, Hercules E Mavrakis, Eleutherios M Kallergis, Emmanuel N Simantirakis, Stavros I Crysostomakis, Ilias G Saloustros, Gregory I Chlouverakis, Panos E Vardas
PURPOSE: The aim of the current study is to disseminate long-term "real-world" data on mortality and device therapies in primary and secondary prevention implantable cardioverter defibrillator (ICD) recipients on the island of Crete. METHODS: We analyzed data for all consecutive patients who received an ICD in our tertiary university hospital from 1993 until December 2013. Follow-up visits were performed every 6 months or more frequently when indicated. Survival status was recorded, and all stored episodes during interrogation were registered and classified as appropriate or inappropriate...
August 20, 2016: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/27621547/effect-of-potentially-chromogenic-beverages-on-shear-bond-strength-of-acrylic-denture-teeth-to-heat-polymerized-denture-base-resins
#11
Karin Hermana Neppelenbroek, Vanessa Migliorini Urban, Denise Gusmão de Oliveira, Vinícius Carvalho Porto, Hercules Jorge Almilhatti, Nara Hellen Campanha
BACKGROUND: Detachment of denture acrylic resin artificial teeth from denture base resin is one of the most common problems presented by denture wearers. PURPOSE: This study investigated the shear bond strength (SBS) and fracture type of bonding interface of two commercial acrylic teeth (Vipi Dent Plus e Biolux) to two denture base resins (Vipi Cril e Lucitone 550) after immersion in potentially chromogenic beverages (coffee, cola soft drink, and red wine) or control solution (distilled water)...
July 2016: Journal of Indian Prosthodontic Society
https://www.readbyqxmd.com/read/27618432/prevalence-and-risks-of-syphilis-among-commercial-sex-workers-in-georgetown-guyana
#12
R Kurup, A C Hercules
Objective: To determine the key affected populations (KAP) and prevalence of syphilis and to investigate the sexual health practices and constrains involved among commercial sex workers (CSWs) in Guyana. Methods: The participants were randomly selected from CSWs participating in support group meetings held in Georgetown and Berbice. The survey was cross-sectional and SPSS was used to perform the data analysis. Results: The majority (92.9%) of CSW were tested negative for syphilis while two (2...
June 4, 2015: West Indian Medical Journal
https://www.readbyqxmd.com/read/27597513/alterations-in-systemic-levels-of-th1-th2-and-th17-cytokines-in-overweight-adolescents-and-obese-mice
#13
Christian Jung, Michael Lichtenauer, Daniela Strodthoff, Holger Winkels, Bernhard Wernly, Christina Bürger, Uran Kamchybekov, Esther Lutgens, Hans-Reiner Figulla, Norbert Gerdes
BACKGROUND: Obesity represents a major problem for patients and health care systems in most industrialized countries. A chronic inflammatory state in obese individuals leads to disease conditions associated with activation of cellular immune mechanisms. Here, we sought to investigate the role of Th1-, Th2-, and Th17-related cytokines in overweight adolescents and mice on a high-fat diet. METHODS: Plasma samples were obtained from 79 male adolescents aged 13-17 years...
September 6, 2016: Pediatric Diabetes
https://www.readbyqxmd.com/read/27561203/selection-and-validation-of-reliable-reference-genes-for-rt-qpcr-analysis-in-a-large-cohort-of-pituitary-adenomas
#14
Kjersti Ringvoll Normann, Kristin Astrid Berland Øystese, Jens Petter Berg, Tove Lekva, Jon Berg-Johnsen, Jens Bollerslev, Nicoleta Cristina Olarescu
BACKGROUND: Real-time reverse transcription quantitative PCR (RT-qPCR) has become the method of choice for quantification of gene expression changes. The most important limitations of RT-qPCR are inappropriate data normalization and inconsistent data analyses. Pituitary adenomas are common tumours, and the appropriate interpretation of increasingly published data within this field is prevented by the lack of a proper selection and validation of stably expressed reference genes. AIM: To find and validate the optimal reference gene or gene combination for reliable RT-qPCR gene expression in both non-functioning (NFPA) and hormone secreting (GH and ACTH) pituitary adenomas...
December 5, 2016: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/27560669/five-year-volumetric-evaluation-of-periodontally-compromised-sites-restored-by-immediate-implant-restorations
#15
Aris Petros Tripodakis, Hercules Gousias, Michael Mastoris, Dionysios Likouresis
The aim of the present study was the retrospective evaluation of the distance of the labial hard and soft tissue margins from the implant shoulder in 24 periodontally compromised sites that were restored by immediate implant restorations. Volumetric analysis was performed 5 years postoperatively using cone beam computed tomography with limited field of view. In all cases, a labial radiopaque plate component was apparent 5 years postoperatively coronal to the implant shoulder (3.1 ± 0.6 mm), supporting the soft tissue margin, which was extending above this level (5...
September 2016: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/27545202/mid-holocene-pulse-of-thinning-in-the-weddell-sea-sector-of-the-west-antarctic-ice-sheet
#16
Andrew S Hein, Shasta M Marrero, John Woodward, Stuart A Dunning, Kate Winter, Matthew J Westoby, Stewart P H T Freeman, Richard P Shanks, David E Sugden
Establishing the trajectory of thinning of the West Antarctic ice sheet (WAIS) since the last glacial maximum (LGM) is important for addressing questions concerning ice sheet (in)stability and changes in global sea level. Here we present detailed geomorphological and cosmogenic nuclide data from the southern Ellsworth Mountains in the heart of the Weddell Sea embayment that suggest the ice sheet, nourished by increased snowfall until the early Holocene, was close to its LGM thickness at 10 ka. A pulse of rapid thinning caused the ice elevation to fall ∼400 m to the present level at 6...
2016: Nature Communications
https://www.readbyqxmd.com/read/27543981/evaluation-of-the-v8-e-class-a-novel-automated-capillary-isoelectric-focusing-instrument-for-hemoglobinopathy-screening
#17
Toon Schiemsky, Caroline Brusselmans, Elke Nackers, Pieter Vermeersch, Koen Poesen, Koen J O Desmet, Nancy Boeckx, Davy M J Kieffer
OBJECTIVES: We evaluated the performance of a novel capillary isoelectric focusing (CIEF) application for hemoglobinopathy screening on the recently introduced V8 E-Class platform. METHODS: Analytical performance of the V8 E-Class was evaluated and included assessment of hemoglobin A2 (HbA2) imprecision; linearity for HbA2, fetal hemoglobin (HbF), and sickle hemoglobin (HbS); and carryover for HbS. Furthermore, a method comparison with the Minicap Flex Piercing (Sebia, Lisses, France), the Variant Classic (Bio-Rad Laboratories, Hercules, CA), and the G8 (Tosoh Europe, Amsterdam, the Netherlands) was done to assess analytical and clinical concordance...
September 2016: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27543816/conventional-and-molecular-methods-used-in-the-detection-and-subtyping-of-yersinia-enterocolitica-in-food
#18
Stefanos Petsios, Maria Fredriksson-Ahomaa, Hercules Sakkas, Chrissanthy Papadopoulou
No abstract text is available yet for this article.
November 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27543509/differential-expression-of-%C3%AE-catenin-egfr-ck7-ck20-muc1-muc2-and-cdx2-in-intestinal-and-pancreatobiliary-type-ampullary-carcinomas
#19
Iraklis Perysinakis, Emilia Minaidou, Vasileia Leontara, Dimitrios Mantas, Georgios C Sotiropoulos, Hercules Tsipras, George N Zografos, Ilias Margaris, Gregory Kouraklis
INTRODUCTION: The purpose of this study was to associate immunohistochemical expression of β-catenin, EGFR, CK7, CK20, MUC1, MUC2, and CDX2 in ampullary adenocarcinomas with the type of differentiation and prognosis. METHODS: Forty-seven patients with ampullary adenocarcinoma who underwent pancreatoduodenectomy with curative intent from 1997 to 2014 were included in this study. Nine patients with perioperative death were included in the association analysis but excluded from survival analysis...
August 19, 2016: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/27535370/large-scale-recording-of-thalamocortical-circuits-in-vivo-electrophysiology-with-the-two-dimensional-electronic-depth-control-silicon-probe
#20
Richárd Fiáth, Patrícia Beregszászi, Domonkos Horváth, Lucia Wittner, Arno A A Aarts, Patrick Ruther, Hercules P Neves, Hajnalka Bokor, Laszlo Acsady, István Ulbert
Recording simultaneous activity of a large number of neurons in distributed neuronal networks is crucial to understand higher order brain functions. Here, we demonstrate the in vivo performance of a recently developed electrophysiological recording system comprising a two-dimensional, multi-shank, high-density silicon probe with integrated CMOS electronics. The system implements the concept of electronic depth control (EDC), which enables the electronic selection of a limited number of recording sites on each of the probe shafts...
August 17, 2016: Journal of Neurophysiology
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