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https://www.readbyqxmd.com/read/28941891/viability-of-and-escherichia-coli-o157-h7-and-listeria-monocytogenes-in-a-delicatessen-appetizer-yogurt-based-salad-as-affected-by-citrus-extract-citrox-%C3%A2-and-storage-temperature
#1
Maria I Tsiraki, Hany M Yehia, Tahra Elobeid, Tareq Osaili, Hercules Sakkas, Ioannis N Savvaidis
The antimicrobial effect of citrus extract (at 1 mL/kg [C1] and 2 mL/kg [C2]) on naturally occurring microbiota and inoculated pathogens (E. coli O157:H7 and L. monocytogenes at ca. 6 log cfu/g) in the traditional Greek yogurt-based salad Tzatziki stored at 4, 10, or 21 °C, was examined. Lactic acid bacteria (LAB) were high (8.0-8.5 log cfu/g) and varied only minimally for both the control (untreated) and the citrus extract-treated salad samples, whereas the higher citrus extract concentration yielded the lowest yeast populations, irrespective of temperature, during the entire storage period...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28918156/exploration-of-the-bovine-colostrum-proteome-and-effects-of-heat-treatment-time-on-colostrum-protein-profile
#2
R Tacoma, S L Gelsinger, Y W Lam, R A Scuderi, D B Ebenstein, A J Heinrichs, S L Greenwood
Heat treatment of colostrum is performed on modern dairy farms to reduce pathogenic contamination before hand-feeding the colostrum to newborn calves; however, limited data are available concerning effects of heat treatment on biologically active proteins in colostrum. The objective of this exploratory study was to investigate effects of heat treatment and length of heat treatment on colostrum protein profile. Colostrum samples were collected from Holstein cows within 12 h after parturition and assigned to the following groups: heat treatment at 60°C for 0 (untreated control), 30, 60, or 90 min...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28899453/application-of-maldi-tof-ms-systems-in-the-rapid-identification-of-campylobacter-spp-of-public-health-importance
#3
Ying-Hsin Hsieh, Yun F Wang, Hercules Moura, Nancy Miranda, Steven Simpson, Ramnath Gowrishankar, John Barr, Khalil Kerdahi, Irshad M Sulaiman
Campylobacteriosis is an infectious gastrointestinal disease caused by Campylobacter spp.In most cases, it is either underdiagnosed or underreported due to poor diagnostics and limited databases. Several DNA-based molecular diagnostic techniques, including 16S ribosomal RNA (rRNA) sequence typing, have been widely used in the species identification of Campylobacter. Nevertheless, these assays are time-consuming and require a high quality of bacterial DNA. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS is an emerging diagnostic technology that can provide the rapid identification of microorganisms by using their intact cells without extraction or purification...
September 12, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28887687/kaiso-is-highly-expressed-in-tnbc-tissues-of-women-of-african-ancestry-compared-to-caucasian-women
#4
Blessing I Bassey-Archibong, Shawn M Hercules, Lyndsay G A Rayner, Desiree H A Skeete, Suzanne P Smith Connell, Ian Brain, Adetola Daramola, Adekunbiola A F Banjo, Jung S Byun, Kevin Gardner, Jonathan Dushoff, Juliet M Daniel
PURPOSE: Triple-negative breast cancer (TNBC) is most prevalent in young women of African ancestry (WAA) compared to women of other ethnicities. Recent studies found a correlation between high expression of the transcription factor Kaiso, TNBC aggressiveness, and ethnicity. However, little is known about Kaiso expression and localization patterns in TNBC tissues of WAA. Herein, we analyze Kaiso expression patterns in TNBC tissues of African (Nigerian), Caribbean (Barbados), African American (AA), and Caucasian American (CA) women...
September 8, 2017: Cancer Causes & Control: CCC
https://www.readbyqxmd.com/read/28858765/1-aryl-1h-and-2-aryl-2h-1-2-3-triazole-derivatives-blockade-p2x7-receptor-in%C3%A2-vitro-and-inflammatory-response-in%C3%A2-vivo
#5
Daniel Tadeu Gomes Gonzaga, Leonardo Braga Gomes Ferreira, Thadeu Estevam Moreira Maramaldo Costa, Natalia Lidmar von Ranke, Paulo Anastácio Furtado Pacheco, Ana Paula Sposito Simões, Juliana Carvalho Arruda, Luiza Pereira Dantas, Hércules Rezende de Freitas, Ricardo Augusto de Melo Reis, Carmen Penido, Murilo Lamim Bello, Helena Carla Castro, Carlos Rangel Rodrigues, Vitor Francisco Ferreira, Robson Xavier Faria, Fernando de Carvalho da Silva
Fifty-one 1,2,3-triazole derivatives were synthesized and evaluated with respect to P2X7 receptor (P2X7R) activity and its associated pore. These triazoles were screened in vitro for dye uptake assay and its cytotoxicity against mammalian cell types. Seven 1,2,3-triazole derivatives (5e, 6e, 8h, 9d, 9i, 11, and 12) potently blocked P2X7 receptor pore formation in vitro (J774.G8 cells and peritoneal macrophages). All blockers displayed IC50 value inferior to 500 nM, and they have low toxicity in either cell types...
August 16, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28838163/using-the-stop-transmission-of-polio-stop-program-to-develop-a-south-sudan-expanded-program-on-immunization-workforce
#6
Dieula D Tchoualeu, Margaret A Hercules, William B Mbabazi, Anthony L Kirbak, Abdulmumini Usman, Ketema Bizuneh, Hardeep S Sandhu
In 2009, the international Stop Transmission of Polio (STOP) program began supporting the Global Polio Eradication Initiative in the Republic of South Sudan to address shortages of human resources and strengthen acute flaccid paralysis surveillance. Workforce capacity support is provided to the South Sudan Expanded Program on Immunization by STOP volunteers, implementing partners, and non-governmental organizations. In 2013, the Polio Technical Advisory Group recommended that South Sudan transition key technical support from external partners to national staff as part of the Polio Eradication and Endgame Strategic Plan, 2013-2018...
July 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28834572/multiple-genetic-variants-associated-with-posttransplantation-diabetes-mellitus-in-chinese-han-populations
#7
Jie Chen, Lixin Li, Yunfei An, Junlong Zhang, Yun Liao, Yi Li, Lanlan Wang
OBJECTIVES: Posttransplantation diabetes mellitus (PTDM) is a major complication after solid organ transplantation. This study is to investigate the association of nine genetic variant factors and PTDM in Chinese Han patients. METHODS: HLA-DP (rs3077, rs9277535), HLA-DQ (rs7453920), signal transducer and activator of transcription 4 (STAT4) (rs7574865), IL-28B (rs12979860, rs8099917, and rs12980275), and IL-18 (rs1946518 and rs187238) were investigated in 260 liver transplant recipients (PTDM vs non-PTDM) by high-resolution melting curve analysis...
August 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28803462/structural-characterization-of-methylenedianiline-regioisomers-by-ion-mobility-mass-spectrometry-tandem-mass-spectrometry-and-computational-strategies-3-maldi-spectra-of-2-ring-isomers
#8
Sarah M Stow, Tiffany M Crescentini, Jay G Forsythe, Jody C May, John A McLean, David M Hercules
Characterization of methylenedianiline (MDA) 2-ring isomers (2,2'-, 2,4'-, and 4,4'-MDA) is reported using matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS), a common technique used for characterizing synthetic polymers. MDA is a precursor to methylene diphenyl diisocyanate (MDI), a hard block component in polyurethane (PUR) synthesis. This work focuses on comparing MALDI results to those of our previous electrospray ionization-mass spectrometry (ESI-MS) studies. In ESI, 2-ring MDA isomers formed single unique [M + H](+) (199 Da) parent ions, whereas in MALDI each isomer shows significant formation of three precursor ions: [M - H](+) = 197 Da, [M(•)](+) = 198 Da, and [M + H](+) = 199 Da...
August 30, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28797594/rare-endoparasitic-asteriomyzostomum-annelida-asteriomyzostomidae-from-japan-including-three-new-species-descriptions-and-their-phylogenetic-position-within-myzostomida
#9
Naoto Jimi, Takeya Moritaki, Hiroshi Kajihara
The rare myzostomid genus Asteriomyzostomum Jägersten, 1940 consists of two species, both parasitizing sea stars. The phylogenetic position of this genus among Myzostomida has not been previously shown using molecular data. In this study, three species of Asteriomyzostomum were collected from the Kumano Sea, Japan, and are described as A. jinshou sp. nov., A. monroeae sp. nov., and A. hercules sp. nov. Additional specimens of the genus Asteromyzostomum were also collected from the Kumano Sea and briefly reported as Asteromyzostomum sp...
August 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28686542/polyunsaturated-fatty-acids-and-endocannabinoids-in-health-and-disease
#10
Hércules Rezende Freitas, Alinny Rosendo Isaac, Renato Malcher-Lopes, Bruno Lourenço Diaz, Isis Hara Trevenzoli, Ricardo Augusto De Melo Reis
Polyunsaturated fatty acids (PUFAs) are lipid derivatives of omega-3 (docosahexaenoic acid, DHA, and eicosapentaenoic acid, EPA) or of omega-6 (arachidonic acid, ARA) synthesized from membrane phospholipids and used as a precursor for endocannabinoids (ECs). They mediate significant effects in the fine-tune adjustment of body homeostasis. Phyto- and synthetic cannabinoids also rule the daily life of billions worldwide, as they are involved in obesity, depression and drug addiction. Consequently, there is growing interest to reveal novel active compounds in this field...
July 7, 2017: Nutritional Neuroscience
https://www.readbyqxmd.com/read/28678925/gestational-protein-restriction-increases-cardiac-connexin-43-mrna-levels-in-male-adult-rat-offspring
#11
Kamila Fernanda Rossini, Camila Andrea de Oliveira, Hércules Jonas Rebelato, Marcelo Augusto Marreto Esquisatto, Rosana Catisti
Background: The dietary limitation during pregnancy influences the growth and development of the fetus and offspring and their health into adult life. The mechanisms underlying the adverse effects of gestational protein restriction (GPR) in the development of the offspring hearts are not well understood. Objectives: The aim of this study was to evaluate the effects of GPR on cardiac structure in male rat offspring at day 60 after birth (d60). Methods: Pregnant Wistar rats were fed a normal-protein (NP, 17% casein) or low-protein (LP, 6% casein) diet...
July 2017: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28619069/social-determinants-of-depression-and-suicidal-behaviour-in-the-caribbean-a-systematic-review
#12
Catherine R Brown, Ian R Hambleton, Natasha Sobers-Grannum, Shawn M Hercules, Nigel Unwin, E Nigel Harris, Rainford Wilks, Marlene MacLeish, Louis Sullivan, Madhuvanti M Murphy
BACKGROUND: Depressive disorder is the largest contributor to years lived with disability in the Caribbean, adding 948 per 100,000 in 2013. Depression is also a major risk factor for suicidal behaviour. Social inequalities influence the occurrence of depression, yet little is known about the social inequalities of this condition in the Caribbean. In support of the 2011 Rio Political Declaration on addressing health inequities, this article presents a systematic review of the role of social determinants on depression and its suicidal behaviours in the Caribbean...
June 15, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28610076/reinstatement-of-i-spirostreptus-i-i-kandyanus-i-humbert-1865-and-deletion-of%C3%A2-i-thyropygus-i-i-allevatus-i-karsch-1881-and-i-sphaeropoeus-i-i-hercules-i-brandt-1833-in-the-checklist-of-sri-lankan-diplopoda
#13
H K S DE Zoysa, Kanishka D B Ukuwela, S Wickramasinghe, Anh D Nguyen, John Hollier
The Diplopoda have been studied for some 250 years, but until the later part of the nineteenth century most descriptions were rather cursory and neither the species concept nor type concept were well established (Hoffman 2009). Even for well-explored regions like Europe, the description of the Diplopoda saw a proliferation of synonyms (Enghoff 1976) while the complexity of Diplopoda systematics was severely underestimated until very recently (Brewer et al. 2012).
March 27, 2017: Zootaxa
https://www.readbyqxmd.com/read/28601386/detection-of-alk-and-kras-mutations-in-circulating-tumor-dna-of-patients-with-advanced-alk-positive-nsclc-with-disease-progression-during-crizotinib-treatment
#14
Paola Bordi, Marcello Tiseo, Eleonora Rofi, Iacopo Petrini, Giuliana Restante, Romano Danesi, Marzia Del Re
BACKGROUND: In patients with anaplastic lymphoma kinase (ALK)-positive non-small-cell lung cancer (NSCLC), disease progression occurs after a median of 9 to 10 months of crizotinib treatment. Several mechanisms of resistance have been identified and include ALK mutations and amplification or the activation of bypassing signaling pathways. Rebiopsy in NSCLC patients represents a critical issue and the analysis of circulating cell-free DNA (cfDNA) has a promising role for the identification of resistance mechanisms...
May 18, 2017: Clinical Lung Cancer
https://www.readbyqxmd.com/read/28600230/cytokine-and-chemokine-profile-changes-in-patients-with-lower-segment-lumbar-degenerative-spondylolisthesis
#15
Juraj Sutovsky, Martin Benco, Martina Sutovska, Michaela Kocmalova, Lenka Pappova, Juraj Miklusica, Andrej Frano, Egon Kurca
BACKGROUND: Lumbar degenerative spondylolisthesis (DS) develops as a result of inflammatory and remodeling processes in facet joints (FJs). Several inflammatory cytokines are involved in the osteoarthritic and remodeling changes that occur and in low-back and/or radicular pain, the most prevalent clinical symptom of disease. This study improves knowledge related to the roles that 27 cytokines, chemokines and growth factors play in the pathophysiology of lumbar DS. MATERIAL AND METHODS: Cytokine levels were examined using capture sandwich immunoassay using the Bio-Plex(®) 200 System and the Bio-PlexTM Human Cytokine Standard 27-Plex, Group I (Bio-Rad, Hercules, California, USA) separately in intervertebral discs (IVDs) and FJ bone tissue...
June 6, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28586698/multiscale-characterization-of-the-hierarchical-structure-of-dynastes-hercules-elytra
#16
Charles Roux-Pertus, Erwan Oliviero, Véronique Viguier, Frédéric Fernandez, Frédéric Maillot, Olivier Ferry, Solenne Fleutot, João F Mano, Franck Cleymand
Beetle elytra are thickened forewings, they are lightweight and tough to protect the hindwings without hindering flight capacities. Dynastes hercules elytra are known for their hygrochromic properties. However, the whole structure of the elytron remains to be characterized. Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and to our knowledge for the first time X-Ray tomography were undertaken on adult male Dynastes hercules to characterize their multi-scale structure. Trabeculae present a periodic arrangement over a short distance...
May 4, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28573491/p2x7-receptor-large-pore-signaling-in-avian-m%C3%A3-ller-glial-cells
#17
Robson X Faria, Hercules R Freitas, Ricardo A M Reis
ATP is a pleiotropic molecule that promotes extra- and intracellular signaling to regulate numerous functions. This nucleotide activates purine and pyrimidine receptors at the plasma membrane, categorized as ionotropic P2X or G-protein-coupled receptor (GPCR) P2Y receptors. P2X are ligand-gated ion channel receptors, expressed in both retinal neurons and Müller cells leading to neuron-glia communication, calcium waves and neurovascular coupling. However, how P2X pore is formed upon ATP activation and how signaling pathways regulates the complex is still a matter of controversy...
June 1, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/28552867/an-unusual-case-of-sodium-channel-myotonia-with-transient-weakness-upon-initiating-movements-which-is-characteristic-in-becker-disease
#18
Junpei Yamamoto, Keiichi Hokkoku, Yuki Hatanaka, Shunichi Sakoda, Jun-Hui Yuan, Masahiro Sonoo
We reported a 32-year-old man who was a sporadic case of myotonic syndrome with muscle stiffness or transient weakness of limbs upon initiating movements after rest. On examination, he showed painless myotonia with warm-up phenomenon, Hercules-like hypertrophic musculature and myotonic discharges in EMG. The clinical findings resembled to those of Becker disease rather than Thomsen disease. But electrodiagnosis suggested sodium channel myotonia instead of chloride channelopathy. Genetic testing detected a novel missense mutation (p...
June 28, 2017: Rinshō Shinkeigaku, Clinical Neurology
https://www.readbyqxmd.com/read/28526460/semi-supervised-medical-entity-recognition-a-study-on-spanish-and-swedish-clinical-corpora
#19
Alicia Pérez, Rebecka Weegar, Arantza Casillas, Koldo Gojenola, Maite Oronoz, Hercules Dalianis
OBJECTIVE: The goal of this study is to investigate entity recognition within Electronic Health Records (EHRs) focusing on Spanish and Swedish. Of particular importance is a robust representation of the entities. In our case, we utilized unsupervised methods to generate such representations. METHODS: The significance of this work stands on its experimental layout. The experiments were carried out under the same conditions for both languages. Several classification approaches were explored: maximum probability, CRF, Perceptron and SVM...
July 2017: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/28503088/restoring-trail-induced-apoptosis-using-naturopathy-hercules-joins-hand-with-nature-to-triumph-over-lernaean-hydra
#20
REVIEW
Ammad Ahmad Farooqi, Cosmo Damiano Gadaleta, Girolamo Ranieri, Sundas Fayyaz, Ilaria Marech
Cancer is a multifaceted disease. Our deepened knowledge about genetic and biological mechanisms of cancer cells presents an opportunity to explore the inter-individual differences in the body's ability to metabolize and respond to different nutrients. It is becoming progressively more understandable that the deregulation of several signaling pathways and the alterations in apoptotic response are some of the major determinants that underpin carcinogenesis. Tumor necrosis factor-Related Apoptosis-Inducing Ligand (TRAIL)-mediated signaling has gained a remarkable appreciation because of its ability to selectively induce apoptosis in cancer cells leaving normal cells intact...
February 2017: Current Genomics
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