Keith P Eidell, Alenka Lovy, Nicholas R Sylvain, Frank A Scangarello, Hayley I Muendlein, Michael J Ophir, Ken Nguyen, Maria-Cristina Seminario, Stephen C Bunnell
Integrin engagement within the immune synapse enhances T cell activation, but our understanding of this process is incomplete. In response to T cell receptor (TCR) ligation, SLP-76 (LCP2), ADAP (FYB1) and SKAP55 (SKAP1) are recruited into microclusters and activate integrins via the effectors talin-1 and kindlin-3 (FERMT3). We postulated that integrins influence the centripetal transport and signaling of SLP-76 microclusters via these linkages. We show that contractile myosin filaments surround and are co-transported with SLP-76 microclusters, and that TCR ligand density governs the centripetal movement of both structures...
August 15, 2021: Journal of Cell Science