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MCAO reperfusion

Jiangsong Zhang, Xianming Lin, Hui Zhou, Yuanyuan Chen, Shuangkai Xiao, Junyue Jiao, Yibin Zhao, Zhong Di
OBJECTIVE: To examine for an opening effect on the blood-brain barrier (BBB) in intact rats and rats with experimental ischaemia-reperfusion (I/R) during the recovery period after various electroacupuncture (EA) treatments with different time courses, and to determine whether there is a time-dependent effect. An additional objective was to determine whether this method could induce the penetration of nerve growth factor (NGF) through the BBB. METHODS: A middle cerebral artery occlusion (MCAO) model was first established...
June 14, 2018: Acupuncture in Medicine: Journal of the British Medical Acupuncture Society
Yu-Chen Fang, Hai-Tong Wan, Li Yu, Fei Yin, Jie-Hong Yang, Hui-Fen Zhou, Yu He
To investigate the pharmacokinetic characteristics of active constituents of Guhong injection in rats with cerebral ischemia reperfusion injury. The middle cerebral artery occlusion (MCAO) model was established in our studies, and then all the rats received iv administration of Guhong injection (2.1 mL·kg⁻¹). The blood concentrations of aceglutamide and hydroxysafflor yellow A (HSYA) were determined by high performance liquid chromatography (HPLC) method at different time points. The concentration-time curves were drawn and pharmacokinetic data were obtained by DAS 3...
May 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Xiaoli Shao, Wangxiao Bao, Xiaoqin Hong, Huihua Jiang, Zhi Yu
Cerebral ischemia/reperfusion (I/R) injury results in detrimental complications. However, little is known about the underlying molecular mechanisms involved in the reperfusion stage. The aim of the present study was to identify a gene expression profile associated with cerebral ischemia/reperfusion injury. The GSE23160 dataset, which comprised data from sham control samples and post‑I/R injury brain tissues that were obtained using a middle cerebral artery occlusion (MCAO) model at 2, 8 and 24 h post‑reperfusion, was downloaded from the Gene Expression Omnibus database...
June 6, 2018: Molecular Medicine Reports
Yuanyuan Ran, Zongjian Liu, Shuo Huang, Jiamei Shen, Fengwu Li, Wenxiu Zhang, Chen Chen, Xiaokun Geng, Zhili Ji, Huishan Du, Xiaoming Hu
Splenectomy before or immediately after stroke provides early brain protection. This study aims to explore the effect of splenectomy on long-term neurological recovery after stroke, which is currently lacking in the field. Adult male rats were randomized into splenectomy or sham groups and then subjected to 90 min of middle cerebral artery occlusion (MCAO). Spleen was removed right upon reperfusion or 3d after MCAO. Infarct volume, neurological functions, and peripheral immune cell populations were assessed up to 28d after stroke...
June 2018: Aging and Disease
Xiangrong Liu, Shaohong Wen, Shunying Zhao, Feng Yan, Shangfeng Zhao, Di Wu, Xunming Ji
Mild therapeutic hypothermia, a robust neuroprotectant, reduces neuronal apoptosis, but the precise mechanism is not well understood. Our previous study showed that a novel inhibitor of an apoptosis-stimulating protein of p53 (iASPP) might be involved in neuronal death after stroke. The aim of this study was to confirm the role of iASPP after stroke treated with mild therapeutic hypothermia. To address this, we mimicked ischemia/reperfusion injury in vitro by using oxygen-glucose deprivation/reperfusion (OGD/R) in primary rat neurons...
June 2018: Aging and Disease
Ziping Han, Haiping Zhao, Zhen Tao, Rongliang Wang, Zhibin Fan, Yumin Luo, Yinghao Luo, Xunming Ji
T-LAK-cell-originated protein kinase (TOPK) is a newly identified member of the mitogen-activated protein kinase family. Our previous study has showed that TOPK has neuroprotective effects against cerebral ischemia-reperfusion injury. Here, we investigated the involvement of TOPK in microglia/ macrophage M1/M2 polarization and the underlying epigenetic mechanism. The expression profiles, co-localization and in vivo interaction of TOPK, M1/M2 surface markers, and HDAC1/HDAC2 were detected after middle cerebral artery occlusion models (MCAO)...
April 2018: Aging and Disease
Caroline Pin-Barre, Christophe Pellegrino, Frédéric Laurin, Jérôme Laurin
The purpose of the present study was to examine the contribution of group III and IV metabosensitive afferents at spinal and supraspinal levels in rats subjected to middle cerebral artery occlusion (MCAO) with reperfusion during the acute phase. Animals were randomized in Control ( n = 23), SHAM ( n = 18), MCAO-D1 ( n = 10), and MCAO-D7 ( n = 20) groups. Rats performed the Electrical Von Frey and the Adhesive removal tests before the surgery and at day 1 (D1), D3, and D7 after MCAO. Animals were subjected to electrophysiological recordings including the responses of group III/IV metabosensitive afferents to combinations of chemical activators and the triceps brachii somatic reflex activity at D1 or D7...
2018: Frontiers in Physiology
Jing Huang, Tao Wang, Daorui Yu, Xingyue Fang, Haofei Fan, Qiang Liu, Guohui Yi, Xinan Yi, Qibin Liu
We investigated the therapeutic effects of l-homocarnosine against inflammation in a rat model of cerebral ischemia-reperfusion injury. Rats were grouped into control, middle cerebral artery occlusion (MCAO), 0.5 mM l-homocarnosine + MCAO, and 1 mM l-homocarnosine + MCAO treatment groups. Superoxide dismutase (SOD), glutathione peroxidase (Gpx), catalase, lipid peroxidation, and reduced glutathione (GSH) levels were measured. Neurological scores were assessed, and histopathology, scanning electron microscopy (SEM), and fluorescence microscopy analyses were conducted...
June 8, 2018: International Journal of Biological Macromolecules
Yasin Asadi, Fazel Gorjipour, Sedigheh Behrouzifar, Abedin Vakili
Evidence has shown therapeutic potential of irisin in cerebral stroke. The present study aimed to assess the effects of recombinant irisin on the infarct size, neurological outcomes, blood-brain barrier (BBB) permeability, apoptosis and brain-derived neurotrophic factor (BDNF) expression in a mouse model of stroke. Transient focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO) for 45 min and followed reperfusion for 23 h in mice. Recombinant irisin was administrated at doses of 0...
June 7, 2018: Neurochemical Research
Tomonobu Kodama, Keisuke Kamata, Koichi Fujiwara, Manabu Kano, Toshitaka Yamakawa, Ichiro Yuki, Yuichi Murayama
Although early reperfusion therapy is effective for acute ischemic stroke, limited therapeutic time-window resulted in only 10% of patients receiving reperfusion therapy. A fast and reliable stroke detection method is desired so that patients can receive early reperfusion therapy. It has been reported that ischemic stroke affects heart rate variability (HRV), which reflects activities of the autonomic nervous function. Thus, ischemic stroke may be detected at an acute stage through monitoring HRV. This paper proposes an HRV-based ischemic stroke detection algorithm by using multivariate statistical process control (MSPC), which is a well-known anomaly detection algorithm...
June 2018: IEEE Transactions on Neural Systems and Rehabilitation Engineering
Yanzhe Wang, Lei Li, Shumin Deng, Fang Liu, Zhiyi He
Toll-like receptors (TLRs) play key roles in cerebral ischemia and reperfusion injury by inducing the production of inflammatory mediators, such as interleukins (ILs) and tumor necrosis factor-alpha (TNF-α). According to recent studies, ursolic acid (UA) regulates TLR signaling and exhibits notable anti-inflammatory properties. In the present study, we explored the mechanism by which UA regulates inflammation in the rat middle cerebral artery occlusion and reperfusion (MCAO/R) model. The MCAO/R model was induced in male Sprague-Dawley rats (MCAO for 2 h, followed by reperfusion for 48 h)...
2018: Frontiers in Neurology
Li Yu, Hao-Fang Wan, Chang Li, Jie-Hong Yang, Hui-Fen Zhou, Hai-Tong Wan, Yu He
Background and Objectives: Guhong Injection (GHI) is usually administered for the treatment of stroke in clinics. Aceglutamide and hydroxyl safflower yellow A (HSYA) are its key ingredients for brain protective effect. To investigate the pharmacokinetics of aceglutamide and HSYA under pathological and normal conditions, the pharmacokinetic parameters and characteristics of middle cerebral artery occlusion (MCAO) and normal rats given the same dosage of GHI were studied compared. Methods: 12 SD rats were divided into two groups, namely, MCAO and normal groups...
2018: Frontiers in Pharmacology
Xia Liu, Geza K Kiss, Scott J Mellender, Harvey R Weiss, Oak Z Chi
Activation of Akt has been suggested to produce neuronal protection in cerebral ischemia. Decreasing blood-brain barrier (BBB) disruption has been associated with a better neuronal outcome in cerebral ischemia. We hypothesized that activation of Akt would decrease BBB disruption and contribute to decreasing the size of infarct in the early stage of cerebral ischemia-reperfusion within the therapeutic window. Transient middle cerebral artery occlusion (MCAO) was performed in rats under isoflurane anesthesia with controlled ventilation...
May 30, 2018: Neuroscience Letters
Jing Yu, Xue Li, Nathanael Matei, Devin McBride, Jiping Tang, Min Yan, John H Zhang
Autophagy activation exerts neuroprotective effects in the ischemic stroke model. Ezetimibe (Eze), a Niemann-Pick disease type C1-Like 1 (NPC1L1) pharmacological inhibitor, has been reported to protect hepatocytes from apoptosis via autophagy activation. In this study, we explored whether Eze could attenuate neuronal apoptosis in the rat model of middle cerebral artery occlusion (MCAO), specifically via activation of the AMPK/ULK1/autophagy pathway. Two hundred and one male Sprague-Dawley rats were subjected to transient MCAO followed by reperfusion...
May 28, 2018: Experimental Neurology
Chunguo Wang, Chenyue Liu, Min Wang, Quantao Ma, Yaqi Li, Ting Wang, Baosheng Zhao
Kudiezi injection (KDZI), also known as Diemailing injection, is a traditional Chinese medicine injection of the composite plant Ixeris sonchifolia Hance (also known as Kudiezi), and has been widely used to treat coronary heart disease, angina pectoris, and cerebral infarction, but its pharmacological mechanisms remain unclear. This study is designed to explore the effects of KDZI on middle cerebral artery occlusion and reperfusion (MCAO/R) rats, and to identify metabolic features of cerebral ischemia reperfusion by using a nontargeted metabolic profiling method based on ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)...
May 30, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Run-Hao Jiang, Xiao-Quan Xu, Chen-Jiang Wu, Shan-Shan Lu, Qing-Quan Zu, Lin-Bo Zhao, Sheng Liu, Hai-Bin Shi
OBJECTIVE: The role of CD40/CD40 ligand (CD40L) in microvascular thrombosis is now widely accepted. However, the exact mechanisms linking the CD40/CD40L system and the soluble form of CD40L (sCD40L) with microvascular thrombosis are currently a topic of intensive research. The objective of this study was to assess the potential mechanisms in CD40/CD40L system-regulated microvascular thrombosis after focal ischemia/reperfusion (I/R). METHODS: Rats were subjected to 60-min transient middle cerebral artery occlusion (MCAO)...
May 29, 2018: Neurological Research
Li Wu, Zeming Ye, Ying Pan, Xianliang Li, Xian Fu, Bo Zhang, Youfu Li, Wanrong Lin, Xuelong Li, Qingchun Gao
BACKGROUND/OBJECTIVE: Vascular endothelial growth factor (VEGF) has been recognized to be a potential pharmaceutical target for treating ischemic stroke, but its severe side effects hinder its widely application. Here, the present study was designed to investigate the effects of VEGF on blood-brain-barrier (BBB) disruption and the underlying mechanisms. METHODS: A mouse model of middle cerebral artery occlusion (MCAO) was constructed and treated with or without VEGF...
May 26, 2018: Experimental Cell Research
Zhe Wang, Yu Zhou, Yan Yu, Ke He, La-Mei Cheng
BACKGROUND: The inflammatory reaction of the spleen is an important component in the pathophysiology of cerebral ischaemia (CI). Regulatory B cells (Bregs) derived from the spleen can inhibit the expansion of inflammation and reduce the damage caused by CI. AIM: The aim of the present study was to explore changes in spleen function and Bregs production due to lipopolysaccharide preconditioning (LPS PC) in ischaemia/reperfusion (I/R) and to uncover potential protective effect of LPS PC on stroke...
May 24, 2018: Brain Research
Yongqiu Zheng, Zhenzhen Wu, Frank Yi, Matthew Orange, Mingjiang Yao, Bin Yang, Jianxun Liu, Hua Zhu
BACKGROUND/AIMS: Ischemic stroke is a leading cause of long-term disability. To date, there is no effective treatment for stroke. Previous studies have shown that Ginkgo biloba extract has protective effects against neurodegenerative disorders. In this present study, we sought to test the potential protective role of an active component of Ginkgo biloba extract, bilobalide, in a rat model of middle cerebral artery occlusion (MCAO). METHODS: A rat model of MCAO was used to test the potential protective effects of Bilobalide B on stroke protection...
May 22, 2018: Cellular Physiology and Biochemistry
Jian-Ying Zhang, Qing-Ke Bai, Ying-Dong Zhang
Inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductases, collectively known as statins, have been shown to minimize cerebral ischemic events in patients. We assessed the mechanisms of simvastatin pretreatment in preventing cerebral ischemia/reperfusion injury in rats using a model of middle cerebral artery occlusion (MCAO). Rats were pretreated with simvastatin 14 days prior to MCAO induction. At 3, 24, and 48 hours after reperfusion, bradykinin levels in the ischemic penumbra were assayed by ELISA, mRNA levels of bradykinin B2 receptors (BK-2Rs) and CD11b were measured by fluorescent quantitative real-time PCR (RT-PCR), and co-expression of microglia and BK-2Rs was determined by immunofluorescence...
November 1, 2017: Journal of Biomedical Research
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