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https://www.readbyqxmd.com/read/29155472/experimental-reconstruction-of-double-strand-break-repair-mediated-plastid-dna-insertion-into-the-tobacco-nucleus
#1
Dong Wang, Jinbao Gu, Rakesh David, Zhen Wang, Songtao Yang, Iain R Searle, Jian-Kang Zhu, Jeremy N Timmis
The mitochondria and plastids of eukaryotic cells evolved from endosymbiotic prokaryotes. DNA from the endosymbionts has bombarded nuclei since the ancestral prokaryotes were engulfed by a precursor of the nucleated eukaryotic host. An experimental confirmation regarding the molecular mechanisms responsible for organelle DNA incorporation into nuclei has not been performed until the present analysis. Here we introduced double-strand DNA breaks into the nuclear genome of tobacco through inducible expression of I-SceI, and showed experimentally that tobacco chloroplast DNAs insert into nuclear genomes through double-strand DNA break repair...
November 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29155129/nervonoylceramide-c24-1cer-a-lipid-biomarker-for-ocular-irritants-released-from-the-3d-reconstructed-human-cornea-like-epithelium-mctt-hce%C3%A2
#2
Miri Lee, Kyung-Mi Joo, Seunghye Choi, Su-Hyon Lee, Seol Yeong Kim, Young-Jin Chun, Dalwoong Choi, Kyung-Min Lim
Due to invasive and painful procedures during in vivo rabbit eye irritation test, in vitro alternative methods have been widely investigated. Recently, 3D reconstructed human cornea-like epitheliums (RhCE) garner a huge attention. RhCEs employ the tissue viability as a primary endpoint to determine ocular irritancy but additional biomarkers may improve its predictive capacity. Here, we explored lipid biomarkers for ocular irritants in MCTT HCE™ RhCE model. Three irritants; sodium lauryl sulfate, benzalkonium chloride and triton X-100 were selected to represent anionic, cationic and non-ionic detergent respectively...
November 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29153581/novel-application-of-micro-computerized-tomography-for-morphologic-characterization-of-the-murine-penis
#3
Marisol O'Neill, Gene O Huang, Dolores J Lamb
BACKGROUND: The murine penis model has enriched our understanding of anomalous penile development. The morphologic characterization of the murine penis using conventional serial sectioning methods is labor intensive and prone to errors. AIM: To develop a novel application of micro-computerized tomography (micro-CT) with iodine staining for rapid, non-destructive morphologic study of murine penis structure. METHODS: Penises were dissected from 10 adult wild-type mice and imaged using micro-CT with iodine staining...
November 16, 2017: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/29153422/a-flap-based-on-the-plantar-digital-artery-arch-branch-to-improve-appearance-of-reconstructed-fingers-anatomical-and-clinical-application
#4
Lin-Feng Tang, Ji-Hui Ju, Yue-Fei Liu, Bo Lan, Rui-Xing Hou
AIM: To investigate blood supply features of the flap based on the plantar digital artery arch and arch branch artery, and the treatment of outcomes of reconstructed fingers by the plantar digital artery arch branch island flap. METHODS: Eight fresh foot specimens were employed with red emulsion infusion and microdissection. The vascular organization was observed in the second toe, such as initiation site, the course, and the number of the plantar digital artery arch branch...
November 16, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29153360/magnetic-resonance-imaging-of-intracranial-atherosclerosis-comparison-of-ex-vivo-3t-mri-and-histologic-findings
#5
Gyung Ho Chung, Hyo Sung Kwak, Seung Bae Hwang, Sang Jae Noh
PURPOSE: Imaging the lipid-rich necrotic core (LRNC) is very important when evaluating the response of lipid-lowering therapy. The purpose of this study was to assess ex vivo LRNC of intracranial atherosclerosis using 3T MRI. MATERIALS AND METHODS: Thirty-one atherosclerotic lesions from 17 specimens were analyzed (basilar artery=15, middle cerebral artery=16) using 3T MRI. Specimens were not chemically processed for imaging studies. Reconstructed MRI was matched with histologic sections at corresponding locations...
December 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/29153358/impact-of-noise-optimized-virtual-monoenergetic-dual-energy-computed-tomography-on-image-quality-in-patients-with-renal-cell-carcinoma
#6
Simon S Martin, Julian L Wichmann, Sophia Pfeifer, Doris Leithner, Lukas Lenga, Michael A Reynolds, Tommaso D'Angelo, Renate Hammerstingl, Tatjana Gruber-Rouh, Thomas J Vogl, Moritz H Albrecht
OBJECTIVE: The aim of this study was to evaluate the impact of a noise-optimized virtual monoenergetic imaging (VMI+) reconstruction technique on image quality and lesion delineation in patients with renal cell carcinoma (RCC) undergoing abdominal dual-energy computed tomography (DECT). MATERIALS AND METHODS: Fifty-two patients (33 men; 61.5±13.6years) with RCC underwent contrast-enhanced DECT during the corticomedullary and nephrogenic phase of renal enhancement...
December 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/29152744/hirano-s-cover-body-model-and-its-unique-laryngeal-postures-revisited
#7
Andrew M Vahabzadeh-Hagh, Zhaoyan Zhang, Dinesh K Chhetri
OBJECTIVES/HYPOTHESIS: In 1974, Minoru Hirano proposed his theory of voice production that is now known as the cover-body theory. He described the thyroarytenoid (TA) and cricothyroid (CT) muscles as the major determinants of vocal fold shape and stiffness, and theorized four typical laryngeal configurations resulting from unique TA/CT activations, with implications for the resulting voice quality. In this study, we directly observed the vocal fold medial surface shape under Hirano's unique TA/CT activation conditions to obtain a three-dimensional (3D) understanding of these laryngeal configurations during muscle activation...
November 20, 2017: Laryngoscope
https://www.readbyqxmd.com/read/29152642/variability-of-characteristics-of-cranial-projectile-trauma-in-skeletal-material
#8
Caitlin Humphrey, Jaliya Kumaratilake, Maciej Henneberg
In cranial firearm trauma, where soft tissues have decomposed, determining the manner of death can pose difficultly for a forensic anthropologist. Detailed analysis of skeletal wounds may lead to reconstruction of the events surrounding death, however considerable research has been conducted on soft tissue details and less has focused on skeletal wounds alone. Macroscopic descriptive observations were carried out on thirteen skulls from the Hamman-Todd Human Osteological Collection at the Cleveland Museum of Natural History, Ohio which had documented cranial projectile trauma to analyse wound location, shape, size, beveling and projectile paths...
November 14, 2017: Anthropologischer Anzeiger; Bericht über die Biologisch-anthropologische Literatur
https://www.readbyqxmd.com/read/29152521/isokinetic-strength-deficit-6-months-after-adductor-canal-blockade-for-anterior-cruciate-ligament-reconstruction
#9
James E Christensen, Natalie E Taylor, Scott J Hetzel, John A Shepler, Tamara A Scerpella
Background: Recent evidence shows a delayed return to sport in children and delayed quadriceps recovery in both adults and children who have undergone anterior cruciate ligament (ACL) reconstruction with concomitant femoral nerve blockade (FNB) compared with those who had no blockade. We evaluated the use of adductor canal blockade (ACB), as an alternative to FNB, at the time of ACL reconstruction. Hypothesis: Patients who receive ACB will have greater isokinetic strength at 6 months postoperative compared with patients who receive FNB at the time of ACL reconstruction...
November 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29151883/speciation-history-of-a-species-complex-of-primulina-eburnea-gesneriaceae-from-limestone-karsts-of-southern-china-a-biodiversity-hot-spot
#10
Jing Wang, Bin Ai, Hanghui Kong, Ming Kang
Limestone karsts in southern China are characterized by high edaphic and topographic heterogeneity and host high levels of species richness and endemism. However, the evolutionary mechanisms for generating such biodiversity remain poorly understood. Here, we performed species delimitation, population genetic analyses, simulations of gene flow and analyses of floral morphological traits to infer the geographic history of speciation in a species complex of Primulina eburnea from limestone karsts of southern China...
October 2017: Evolutionary Applications
https://www.readbyqxmd.com/read/29151547/-the-effect-of-reconstruction-method-on-the-ability-to-analyze-of-ffr-using-ct
#11
Hidekazu Inage, Yosuke Kogure, Kanako Kumamaru, Shinichiro Fujimoto, Kazuhisa Takamura, Yuko Kawaguchi, Htun Han Ni, Haruyoshi Hoshito
BACKGROUND: Invasive-fractional flow reserve (FFR) is the reference standard to evaluate functional ischemia of coronary arteries, and is used to decide if percutaneous transluminal coronary angioplasty is necessary. Recently, computed tomography-derived FFR (CT-FFR) is emerged as an alternative non-invasive method. OBJECTIVES: To evaluate the effect of reconstruction methods and image parameters on the accuracy of CT-FFR calculation. METHODS: A total of 26 segments in the consecutive 10 coronary CT angiography (CCTA) studies were evaluated...
2017: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/29151368/the-application-of-scanning-electron-microscopy-with-energy-dispersive-x-ray-spectroscopy-sem-edx-in-ancient-dental-calculus-for-the-reconstruction-of-human-habits
#12
Dana Fialová, Radim Skoupý, Eva Drozdová, Aleš Paták, Jakub Piňos, Lukáš Šín, Radoslav Beňuš, Bohuslav Klíma
The great potential of scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) is in detection of unusual chemical elements included in ancient human dental calculus to verify hypotheses about life and burial habits of historic populations and individuals. Elemental spectra were performed from archeological samples of three chosen individuals from different time periods. The unusual presence of magnesium, aluminum, and silicon in the first sample could confirm the hypothesis of high degree of dental abrasion caused by particles from grinding stones in flour...
November 20, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/29150982/visualization-within-the-ventricles-of-the-brain-a-micro-focus-x-ray-study
#13
Edwin John de Jager, André Francois du Plessis, Jakobus Willem Hoffman, Anna Catherina Oettlé, Marius Casparus Bosman
Conceptualization of the ventricular system of the brain by macroscopic studies is complicated by the lack of physical structure of these interconnected cavities. Dissection procedures designed to display the structures in the walls of the ventricles are destructive and not conducive for the appreciation of the ventricular system in its entirety. The application of Micro-focus X-ray tomography affords the possibility to appreciate hidden structures in a non-destructive manner. The aim of this study was to explore the possibility of using micro-focus X-ray tomography in the three-dimensional (3D) visualization of the ventricular system as well as the various neuroanatomical structures within its walls for educational purposes...
November 18, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29150683/null-functions-in-three-dimensional-imaging-of-alpha-and-beta-particles
#14
Yijun Ding, Luca Caucci, Harrison H Barrett
Null functions of an imaging system are functions in the object space that give exactly zero data. Hence, they represent the intrinsic limitations of the imaging system. Null functions exist in all digital imaging systems, because these systems map continuous objects to discrete data. However, the emergence of detectors that measure continuous data, e.g. particle-processing (PP) detectors, has the potential to eliminate null functions. PP detectors process signals produced by each particle and estimate particle attributes, which include two position coordinates and three components of momentum, as continuous variables...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150186/articular-scaling-and-body-mass-estimation-in-platyrrhines-and-catarrhines-modern-variation-and-application-to-fossil-anthropoids
#15
Jonathan M G Perry, Siobhán B Cooke, Jacqueline A Runestad Connour, M Loring Burgess, Christopher B Ruff
Body mass is an important component of any paleobiological reconstruction. Reliable skeletal dimensions for making estimates are desirable but extant primate reference samples with known body masses are rare. We estimated body mass in a sample of extinct platyrrhines and Fayum anthropoids based on four measurements of the articular surfaces of the humerus and femur. Estimates were based on a large extant reference sample of wild-collected individuals with associated body masses, including previously published and new data from extant platyrrhines, cercopithecoids, and hominoids...
November 14, 2017: Journal of Human Evolution
https://www.readbyqxmd.com/read/29149846/the-impact-of-bone-morphology-on-the-outcome-of-the-pivot-shift-test-a-cohort-study
#16
Thomas Branch, Shaun Stinton, Adrija Sharma, Frederic Lavoie, Christian Guier, Philippe Neyret
BACKGROUND: The presence of a positive pivot shift after surgical repair of the ACL is considered an important indicator of a failed reconstruction. The ability to predict the result of a pivot shift test after an ACL reconstruction using variables that can be measured prior to surgery could provide an indication of which patients may be at-risk of a poor surgical outcome.The purpose of this study was to determine whether structural characteristics of the femur and tibia, measured using plain radiographs, were associated with the result of the pivot shift test in unilateral ACL reconstructed patients...
November 17, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29149740/skin-reducing-mastectomy-and-direct-to-implant-reconstruction-in-giant-phyllodes-tumour-of-breast-case-report
#17
Francesco Ciancio, Alessandro Innocenti, Luigi Cagiano, Aurelio Portincasa, Domenico Parisi
BACKGROUND: The phyllodes tumour of the breast comprises about 0.5% of all neoplasms of the breast. CASE REPORT: we present the case of a female patient (55 years old) suffering from giant phyllodes tumour (>10cm) of the left breast, who underwent Skin Reducing Mastectomy (SRM) and reconstruction with silicone breast implant (Bostwick Technique). DISCUSSION AND CONCLUSION: In selected patients with large sized breasts (C-D cup) and poor surface area of skin involved, a valid technique in the treatment of this pathology is the Skin-Reducing Mastectomy and immediate reconstruction with implants (DTI=direct to implant)...
November 13, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29149388/problems-of-reconstructive-cranioplasty-after-traumatic-brain-injury-in-children
#18
Paolo Frassanito, Gianpiero Tamburrini, Luca Massimi, Simone Peraio, Massimo Caldarelli, Concezio Di Rocco
Cranial repair after traumatic brain injury in children is still burdened by unsolved problems and controversial issues, mainly due to the high rate of resorption of autologous bone as well as the absence of valid alternative material to replace the autologous bone. Indeed, inert biomaterials are associated to satisfactory results in the short period but bear the continuous risk of complications related to the lack of osteointegration capacity. Biomimetic materials claiming osteoconductive properties that could balance their mechanical limits seem to allow good cranial bone reconstruction...
October 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/29149246/asian-cleft-rhinoplasty-the-open-structural-approach
#19
Chin-Ho Wong, Rollin K Daniel, Seng-Teik Lee
Background: Asian cleft rhinoplasty is a unique procedure with specific challenges. Objectives: This paper presents our experience with the use of an open structural technique for Asian cleft rhinoplasty utilizing a complete autologous approach. Methods: An open approach that reconstructs the malpositioned nasal cartilage was utilized. Centrally, the septocolumella graft fixed securely in the midline with extender spreader grafts was utilized to project and lengthen the nose...
November 15, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29148696/the-epidermal-penetration-of-gold-nanoparticles-and-its-underlying-mechanism-based-on-human-reconstructed-3d-episkin-tm-model
#20
Fang Hao, Xiaoting Jin, Qian S Liu, Qunfang Zhou, Guibin Jiang
Nanomaterials are widely used in diverse aspects and their translocation behavior through the skin would be helpful in understanding the corresponding exposure risks. To reveal how surface functionalization of nanoparticles influence the skin penetration, three kinds of gold nanoparticles (GNPs) with negatively, neutrally and positively charged surfaces, i.e. cetyltrimethyl ammonium bromide-coated GNPs (CTAB@GNP), polyvinyl pyrrolidone-coated GNPs (PVP@GNP), and citrate-coated GNPs (Citrate@GNP), were studied using human reconstructed 3D EpiskinTM model...
November 17, 2017: ACS Applied Materials & Interfaces
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