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https://www.readbyqxmd.com/read/28550448/towards-xeno-free-cultures-of-human-limbal-stem-cells-for-ocular-surface-reconstruction
#1
Zala Lužnik, Claudia Breda, Vanessa Barbaro, Stefano Ferrari, Angelo Migliorati, Enzo Di Iorio, Barbara Ferrari, Carlo Griffoni, Andrea Grassetto, Hossein Mostafa Elbadawy, Marina Bertolin
Isolated limbal epithelial stem cells (LESCs) were cultured with or without a 3T3 murine fibroblast feeder-layer (FL) in 4 different culture media on culture plates or on denuded human amniotic membrane (AM) support and fibrin gel support: (1) control medium supplemented with fetal bovine serum; (2) control medium supplemented with the synthetic serum "XerumFree™ XF205" (XF); (3) CnT-20 medium supplemented with "XerumFree™ XF205" (CnT-XF) and (4) CnT-20 medium supplemented with human AB serum (CnT-AB). The three xenogeneic media were compared to standard condition (control + FL) and parameters assessed included cell morphology, proliferative potential, number of passages, assessment of clonogenic and abortive colonies, life span, ∆Np63α expression and epithelial morphology on AM...
May 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28549540/micro-computed-tomography-of-false-starts-produced-on-bone-by-different-hand-saws
#2
Guido Pelletti, Guido Viel, Paolo Fais, Alessia Viero, Sindi Visentin, Diego Miotto, Massimo Montisci, Giovanni Cecchetto, Chiara Giraudo
The analysis of macro- and microscopic characteristics of saw marks on bones can provide useful information about the class of the tool utilized to produce the injury. The aim of the present study was to test micro-computed tomography (micro-CT) for the analysis of false starts experimentally produced on 32 human bone sections using 4 different hand-saws in order to verify the potential utility of micro-CT for distinguishing false starts produced by different saws and to correlate the morphology of the tool with that of the bone mark...
May 2017: Legal Medicine
https://www.readbyqxmd.com/read/28549521/techniques-of-parenchyma-sparing-hepatectomy-for-the-treatment-of-tumors-involving-the-hepatocaval-confluence-a%C3%A2-reliable-way-to-assure-an-adequate-future-liver-remnant-volume
#3
Lucio Urbani, Piero Colombatto, Riccardo Balestri, Gabriella Licitra, Chiara Leoni, Francesco Forfori, Gianluca Masi, Piero Boraschi, Maura Castagna, Piero Buccianti
BACKGROUND: Parenchyma-sparing hepatectomy techniques allow a lesser volume resection (<3 adjacent segments) for tumors involving the hepatic veins at the hepatocaval confluence, assuring adequate volume of the future liver remnant. We report the ability to perform parenchyma-sparing hepatectomy as planned from the preoperative imaging and the type of vascular intervention used to preserve hepatic outflow. METHODS: We analyzed 60 consecutive parenchyma-sparing hepatectomies in 54 patients for 7 primary and 53 metastatic tumors (48 colorectal), located in segments I, VII, VIII, or IVa and involving the hepatocaval confluence...
May 24, 2017: Surgery
https://www.readbyqxmd.com/read/28549397/assessment-of-distal-radioulnar-joint-stability-after-reconstruction-with-the-brachioradialis-wrap
#4
Charity S Burke, Keith A Zoeller, Seid W Waddell, John A Nyland, Michael J Voor, Amitava Gupta
BACKGROUND: The brachioradialis (BR) wrap technique is an option to restore the stability of the distal radioulnar joint (DRUJ). The technique capitalizes on the BR's advantageous insertion point on the radial styloid and the ability of the BR to be harvested with minimal to no deficit. The tendon can then be wrapped around the radius and ulna, tunneling under the pronator quadratus and extensor compartments and secured back into its insertion to provide stability. In this cadaveric study, we used micro-computed tomography (CT) to assess the stability restored by this procedure...
May 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28549194/disentangling-timing-of-admixture-patterns-of-introgression-and-phenotypic-indicators-in-a-hybridizing-wolf-population
#5
M Galaverni, R Caniglia, L Pagani, E Fabbri, A Boattini, E Randi
Hybridization is a natural or anthropogenic process that can deeply affect the genetic make-up of populations, possibly decreasing individual fitness but sometimes favouring local adaptations. The population of Italian wolves (Canis lupus), after protracted demographic declines and isolation, is currently expanding in anthropic areas, with documented cases of hybridization with stray domestic dogs. However, identifying admixture patterns in deeply introgressed populations is far from trivial. In this study, we used a panel of 170,000 SNPs analysed with multivariate, Bayesian and local ancestry reconstruction methods to identify hybrids, estimate their ancestry proportions and timing since admixture...
May 26, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28548611/relationships-between-knee-extension-moments-during-weighted-and-unweighted-gait-and-strength-measures-that-predict-knee-moments-after-acl-reconstruction
#6
Erin Hartigan, Jennifer Aucoin, Rita Carlson, Melanie Klieber-Kusak, Thomas Murray, Bernadette Shaw, Michael Lawrence
BACKGROUND: Weighted gait increases internal knee extension moment impulses (KEMI) in the anterior cruciate ligament-reconstructed (ACLR) limb; however, limb differences persist. HYPOTHESES: (1) KEMI during normal gait will influence KEMI during weighted gait and (2) peak knee extension (PKE) torque and time to reach PKE torque will predict KEMI during gait tasks. STUDY DESIGN: Descriptive laboratory study. METHODS: Twenty-four women and 14 men completed 3 gait tasks (unweighted, vest, sled) and strength testing after discharge from rehabilitation and clearance to return to sports...
May 1, 2017: Sports Health
https://www.readbyqxmd.com/read/28548240/viscoelastic-computational-modeling-of-the-human-head-neck-system-eigenfrequencies-and-time-dependent-analysis
#7
E Boccia, A Gizzi, C Cherubini, M G C Nestola, S Filippi
A subject-specific three-dimensional viscoelastic finite element model of the human head-neck system is presented and investigated based on Computed Tomography and Magnetic Resonance biomedical images. Ad hoc imaging processing tools are developed for the reconstruction of the simulation domain geometry and the internal distribution of bone and soft tissues. Materials viscoelastic properties are characterized point-wise through an image-based interpolating function used then for assigning the constitutive prescriptions of a heterogenous viscoelastic continuum model...
May 26, 2017: International Journal for Numerical Methods in Biomedical Engineering
https://www.readbyqxmd.com/read/28547586/both-isolated-and-multi-ligament-posterior-cruciate-ligament-reconstruction-results-in-improved-subjective-outcome-results-from-the-danish-knee-ligament-reconstruction-registry
#8
Martin Lind, Torsten Grønbech Nielsen, Kristian Behrndtz
PURPOSE: There are few descriptions of outcomes after posterior cruciate ligament (PCL) reconstruction in the literature due to the rarity of this type of knee injury. Since 2005, the Danish Knee Ligament Reconstruction Registry (DKRR) has monitored outcomes of PCL reconstructions. This study describes the epidemiology of PCL reconstruction and subsequent outcomes in a Danish population. METHODS: Of 23,253 knee ligament reconstructions performed in 2005-2015, 581 were registered as PCL reconstructions in the DKRR...
May 25, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28547248/inferior-glenoid-fossa-fractures-patho-anatomy-and-results-of-operative-treatment
#9
Jan Bartoníček, Michal Tuček, Daniel Klika
INTRODUCTION: A typical feature of inferior glenoid fractures is a distal fragment separated from the glenoid fossa. In most cases, the inferior glenoid fractures are associated with a fracture of the scapular body. However, there are no detailed studies of inferior glenoid fractures, and only brief mentions can be found in the literature in this respect. MATERIAL AND METHODS: The basic group comprised 42 patients, with the mean age of 48 years, who sustained 42 fractures of the inferior glenoid...
May 25, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28546862/block-sparsity-based-joint-compressed-sensing-recovery-of-multi-channel-ecg-signals
#10
Anurag Singh, Samarendra Dandapat
In recent years, compressed sensing (CS) has emerged as an effective alternative to conventional wavelet based data compression techniques. This is due to its simple and energy-efficient data reduction procedure, which makes it suitable for resource-constrained wireless body area network (WBAN)-enabled electrocardiogram (ECG) telemonitoring applications. Both spatial and temporal correlations exist simultaneously in multi-channel ECG (MECG) signals. Exploitation of both types of correlations is very important in CS-based ECG telemonitoring systems for better performance...
April 2017: Healthcare Technology Letters
https://www.readbyqxmd.com/read/28546671/symptomatology-and-surgical-perspective-of-scar-endometriosis-a-case-series-of-16-women
#11
Sudha Sumathy, Janu Mangalakanthi, Kishore Purushothaman, Deepti Sharma, Chithra Remadevi, Sarala Sreedhar
AIM: The prevalence of scar endometriosis is increasing with the increasing caesarean deliveries and laparoscopic procedures done for pelvic endometriosis. To analyse the symptomatology and surgical perspective of scar endometriosis. MATERIALS AND METHODS: Retrospective review of 16 women who underwent surgery for scar endometriosis in the period of 4 years in Amrita institute of medical sciences. RESULTS: Mean age of the patients is 35.19 years...
June 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/28546042/occluded-superficial-femoral-artery-used-for-emergency-reconstruction-a-consecutive-case-series-about-the-proper-conduit-technique
#12
M S Marsman, A Kh Jahrome, F L Moll, G G Koning
INTRODUCTION: Deep wound infections in the groin region can result in an acute or life threatening condition. Especially when there is no suitable vein available because of many reasons (e.g. previous surgery, small vessel diameter). Synthetic prosthesis sometimes are not 'first-choice-technique' because of contamination or infection. It was ad hoc hypothesized in an acute setting that a segment of an occluded Superficial Femoral Artery (SFA) could be used as a conduit in combination with eversion technique, known from carotid surgery...
May 22, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28545684/mri-of-cystic-fibrosis-lung-manifestations-sequence-evaluation-and-clinical-outcome-analysis
#13
O Scholz, T Denecke, J Böttcher, C Schwarz, H-J Mentzel, F Streitparth, M H Maurer, A Pfeil, A Huppertz, A Mehl, D Staab, B Hamm, D M Renz
AIM: To evaluate different magnetic resonance imaging (MRI) sequences for diagnosis of pulmonary manifestations of cystic fibrosis (CF) in comparison to chest computed tomography (CT), including an extended outcome analysis. MATERIALS AND METHODS: Twenty-eight patients with CF (15 male, 13 female, mean age 30.5±9.4 years) underwent CT and MRI of the lung. MRI (1.5 T) included different T2- and T1-weighted sequences: breath-hold HASTE (half Fourier acquisition single shot turbo spin echo) and VIBE (volumetric interpolated breath-hold examination, before and after contrast medium administration) sequences and respiratory-triggered PROPELLER (periodically rotated overlapping parallel lines with enhanced reconstruction) sequences with and without fat signal suppression, and perfusion imaging...
May 22, 2017: Clinical Radiology
https://www.readbyqxmd.com/read/28545379/tissue-engineering-in-achilles-tendon-reconstruction-the-role-of-stem-cells-growth-factors-and-scaffolds
#14
D S Pillai, Bal S Dhinsa, Wasim Khan
Achilles tendon injuries are common, and present a challenge in the acute and chronic setting. There is significant morbidity associated with the injury and the numerous management strategies, as well as financial implications to the patient and the health service. To date, repair tissue from all methods of management fail to achieve the same functional and biomechanical properties as the native tendon. The use of tissue engineering technology may reduce morbidity, improve the biomechanical properties of repair tissue and reduce the financial burden...
May 23, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28545083/simultaneous-inference-of-phylogenetic-and-transmission-trees-in-infectious-disease-outbreaks
#15
Don Klinkenberg, Jantien A Backer, Xavier Didelot, Caroline Colijn, Jacco Wallinga
Whole-genome sequencing of pathogens from host samples becomes more and more routine during infectious disease outbreaks. These data provide information on possible transmission events which can be used for further epidemiologic analyses, such as identification of risk factors for infectivity and transmission. However, the relationship between transmission events and sequence data is obscured by uncertainty arising from four largely unobserved processes: transmission, case observation, within-host pathogen dynamics and mutation...
May 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28544550/post-laryngectomy-stricture-and-pharyngocutaneous-fistula-review-of-techniques-in-primary-pharyngeal-reconstruction-in-laryngectomy
#16
Benjamin Walton, Julian Vellucci, Palak Patel, Kristofer Jennings, Susan McCammon, Michael P Underbrink
OBJECTIVE: The purpose of this study is to find a correlation between closure technique in pharyngeal closure and outcomes of both pharyngocutaneous fistula and post-laryngectomy stricture after laryngectomy. STUDY DESIGN: Retrospective Chart Review. METHODS: We retrospectively reviewed a total of 151 patients over a 20 year period from January 1994 to December of 2013 who underwent primary pharyngeal reconstruction after total laryngectomy specifically looking at the closure technique in relation to pharyngo-cutaneous fistula (PCF) and post-laryngectomy stricture post-operatively...
May 19, 2017: Clinical Otolaryngology
https://www.readbyqxmd.com/read/28544393/comparison-of-5-year-oncological-outcomes-of-breast-cancer-based-on-surgery-type
#17
Jeeyeon Lee, Jin Hyang Jung, Wan Wook Kim, Yee Soo Chae, Soo Jung Lee, Ho Yong Park
BACKGROUND: A standardized classification system for breast surgery that incorporates oncoplastic techniques is needed. We classified the surgical techniques for breast cancer treatment into five groups according to the extent of surgery and reconstructive methods, i.e. conventional breast-conserving surgery, partial mastectomy with volume displacement, partial mastectomy with volume replacement, simple mastectomy and total mastectomy with immediate reconstruction. We then evaluated the oncological outcomes for each of the five groups...
May 21, 2017: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/28543761/experimental-characterization-of-a-direct-conversion-amorphous-selenium-detector-with-thicker-conversion-layer-for-dual-energy-contrast-enhanced-breast-imaging
#18
David A Scadutom, Olivier Tousignant, Wei Zhao
PURPOSE: Dual-energy contrast-enhanced imaging is being investigated as a tool to identify and localize angiogenesis in the breast, a possible indicator of malignant tumors. This imaging technique requires that x-ray images are acquired at energies above the k-shell binding energy of an appropriate radiocontrast agent. Iodinated contrast agents are commonly used for vascular imaging, and require x-ray energies greater than 33 keV. Conventional direct conversion amorphous selenium (a-Se) flat panel imagers for digital mammography show suboptimal absorption efficiencies at these higher energies...
May 21, 2017: Medical Physics
https://www.readbyqxmd.com/read/28543704/comparison-of-2-vs-3-dimensional-fracture-mapping-strategies-for-3-dimensional-computerized-tomography-reconstructions-of-scapula-neck-body-fractures
#19
Anthony J Dugarte, Luisa Tkany, Lisa K Schroder, Andreas Petersik, Peter A Cole
Fracture mapping has been used in the understanding of injury patterns in different bones. To our knowledge, there are no applications of this technique using three-dimensional (3D) morphologic fracture characteristics. Previously, scapula fractures were mapped by transferring information from 3D computed tomography to a two-dimensional (2D) template. Cole et al. determined that 3D Computerized Tomography (CT) scans were more reliable compared to plain radiographs in terms of scapular angulation, translation, and glenopolar angle measurements...
May 19, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28543692/the-impact-of-resident-involvement-in-breast-reconstruction-surgery-outcomes-by-modality-an-analysis-of-4-500-cases
#20
Kevin T Jubbal, Anthony Echo, Aldona J Spiegel, Shayan A Izaddoost
BACKGROUND: The goal of this study was to determine the impact of resident involvement on various methods of breast reconstruction via an American College of Surgeons National Surgical Quality Improvement Program (ACS NSQIP) retrospective analysis. METHODS: We performed a retrospective analysis of the ACS NSQIP database to identify patients undergoing breast reconstruction by free flap, implant, latissimus dorsi (LD), and transverse rectus abdominis myocutaneous (TRAM) flap reconstruction modalities...
May 24, 2017: Microsurgery
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