keyword
MENU ▼
Read by QxMD icon Read
search

outer membrane protein analysis

keyword
https://www.readbyqxmd.com/read/28934165/salmonella-o48-serum-resistance-is-connected-with-the-elongation-of-the-lipopolysaccharide-o-antigen-containing-sialic-acid
#1
Aleksandra Pawlak, Jacek Rybka, Bartłomiej Dudek, Eva Krzyżewska, Wojciech Rybka, Anna Kędziora, Elżbieta Klausa, Gabriela Bugla-Płoskońska
Complement is one of the most important parts of the innate immune system. Some bacteria can gain resistance against the bactericidal action of complement by decorating their outer cell surface with lipopolysaccharides (LPSs) containing a very long O-antigen or with specific outer membrane proteins. Additionally, the presence of sialic acid in the LPS molecules can provide a level of protection for bacteria, likening them to human cells, a phenomenon known as molecular mimicry. Salmonella O48, which contains sialic acid in the O-antigen, is the major cause of reptile-associated salmonellosis, a worldwide public health problem...
September 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28934071/prevalence-and-phylogenetic-analysis-of-orientia-tsutsugamushi-in-rodents-and-mites-from-central-india
#2
Ruchi Bhate, Nilesh Pansare, Sandeep P Chaudhari, Sukhadeo B Barbuddhe, Vijay K Choudhary, Nitin V Kurkure, Sunil W Kolte
Orientia tsutsugamushi, the causative agent of scrub typhus in humans, is an obligate intracytosolic bacterium transmitted among animals and to humans by some species of larval trombiculid mites (chiggers) and is hosted mainly by rodents. In this study, we attempted detection of O. tsutsugamushi from blood and tissue samples of rodents trapped from different locations in Central India using PCR targeting the 56 kDa outer membrane protein gene and the 47 kDa high temperature transmembrane protein gene. A total of 59 rodent samples comprising 38 of blood collected from domestic and public surroundings and 21 of tissue from agricultural farm were included in this study...
September 21, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28923482/the-optimal-regulation-mode-of-bcl-2-apoptotic-switch-revealed-by-bistability-analysis
#3
Zhiyong Yin, Hong Qi, Lili Liu, Zhen Jin
In most cell types, apoptosis occurs by the mitochondrial outer membrane permeability (MOMP)-mediated pathway, which is controlled by Bcl-2 family proteins (often referred to as Bcl-2 apoptotic switch). These proteins, which display a range of bioactivities, can be divided into four types: effectors, inhibitors, activators and sensitizers. Although the complex interactions among Bcl-2 family members have been studied intensively, a unifying hypothesis for the mechanism they use to regulate MOMP remains elusive...
September 15, 2017: Bio Systems
https://www.readbyqxmd.com/read/28919551/identification-of-potential-antigens-from-non-classically-secreted-proteins-and-designing-novel-multitope-peptide-vaccine-candidate-against-brucella-melitensis-through-reverse-vaccinology-and-immunoinformatics-approach
#4
Udayakumar S Vishnu, Jagadesan Sankarasubramanian, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Brucella melitensis is an intracellular pathogen resides in the professional and non-professional phagocytes of the host, causing zoonotic disease brucellosis. The stealthy nature of the Brucella makes it's highly pathogenic, and it is hard to eliminate the bacteria completely from the infected host. Hitherto, no licensed vaccines are available for human brucellosis. In this study, we identified potential antigens for vaccine development from non-classically secreted proteins through reverse vaccinology approach...
September 14, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28916712/identification-of-new-channels-by-systematic-analysis-of-the-mitochondrial-outer-membrane
#5
Vivien Krüger, Thomas Becker, Lars Becker, Malayko Montilla-Martinez, Lars Ellenrieder, F-Nora Vögtle, Helmut E Meyer, Michael T Ryan, Nils Wiedemann, Bettina Warscheid, Nikolaus Pfanner, Richard Wagner, Chris Meisinger
The mitochondrial outer membrane is essential for communication between mitochondria and the rest of the cell and facilitates the transport of metabolites, ions, and proteins. All mitochondrial outer membrane channels known to date are β-barrel membrane proteins, including the abundant voltage-dependent anion channel and the cation-preferring protein-conducting channels Tom40, Sam50, and Mdm10. We analyzed outer membrane fractions of yeast mitochondria and identified four new channel activities: two anion-preferring channels and two cation-preferring channels...
September 15, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28900025/psp-stress-response-proteins-form-a-complex-with-mislocalized-secretins-in-the-yersinia-enterocolitica-cytoplasmic-membrane
#6
Disha Srivastava, Amal Moumene, Josué Flores-Kim, Andrew J Darwin
The bacterial phage shock protein system (Psp) is a conserved extracytoplasmic stress response that is essential for the virulence of some pathogens, including Yersinia enterocolitica It is induced by events that can compromise inner membrane (IM) integrity, including the mislocalization of outer membrane pore-forming proteins called secretins. In the absence of the Psp system, secretin mislocalization permeabilizes the IM and causes rapid cell death. The Psp proteins PspB and PspC form an integral IM complex with two independent roles...
September 12, 2017: MBio
https://www.readbyqxmd.com/read/28888603/potential-factors-involved-in-virulence-of-cronobacter-sakazakii-isolates-by-comparative-transcriptome-analysis
#7
Yingwang Ye, Xiyan Zhang, Maofeng Zhang, Na Ling, Haiyan Zeng, Jina Gao, Rui Jiao, Qingping Wu, Jumei Zhang
Cronobacter species are important foodborne pathogens causing severe infections in neonates through consumption of contaminated powdered infant formula. However, the virulence-associated factors in Cronobacter are largely unknown. In this study, the transcriptome analysis between highly virulent Cronobacter sakazakii G362 and attenuated L3101 strains was used to reveal the potential factors involved in virulence. The total transcripts were grouped into 20 clusters of orthologous group categories and summarized in 3 gene ontology categories (biological process, cellular component, and molecular function)...
September 6, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28880942/live-cell-imaging-to-measure-bax-recruitment-kinetics-to-mitochondria-during-apoptosis
#8
Margaret E Maes, Cassandra L Schlamp, Robert W Nickells
The pro-apoptotic BCL2 gene family member, BAX, plays a pivotal role in the intrinsic apoptotic pathway. Under cellular stress, BAX recruitment to the mitochondria occurs when activated BAX forms dimers, then oligomers, to initiate mitochondria outer membrane permeabilization (MOMP), a process critical for apoptotic progression. The activation and recruitment of BAX to form oligomers has been studied for two decades using fusion proteins with a fluorescent reporter attached in-frame to the BAX N-terminus. We applied high-speed live cell imaging to monitor the recruitment of BAX fusion proteins in dying cells...
2017: PloS One
https://www.readbyqxmd.com/read/28874410/identification-and-characterization-of-the-major-porin-of-desulfovibrio-vulgaris-hildenborough
#9
L Zeng, E Wooton, D A Stahl, P J Walian
Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris Hildenborough (DvH), a model organism for studies of sulfate-reducing bacteria, no porins have been identified or proposed in its annotated genome. Results from initial biochemical studies suggested that the product of gene DVU0799, one of the most abundant proteins of the DvH OM and purified as a homotrimeric complex, was a strong porin candidate...
September 5, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28871245/comparative-genomic-analysis-of-neutrophilic-iron-ii-oxidizer-genomes-for-candidate-genes-in-extracellular-electron-transfer
#10
Shaomei He, Roman A Barco, David Emerson, Eric E Roden
Extracellular electron transfer (EET) is recognized as a key biochemical process in circumneutral pH Fe(II)-oxidizing bacteria (FeOB). In this study, we searched for candidate EET genes in 73 neutrophilic FeOB genomes, among which 43 genomes are complete or close-to-complete and the rest have estimated genome completeness ranging from 5 to 91%. These neutrophilic FeOB span members of the microaerophilic, anaerobic phototrophic, and anaerobic nitrate-reducing FeOB groups. We found that many microaerophilic and several anaerobic FeOB possess homologs of Cyc2, an outer membrane cytochrome c originally identified in Acidithiobacillus ferrooxidans...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28865072/gallibacterium-elongation-factor-tu-possesses-amyloid-like-protein-characteristics-participates-in-cell-adhesion-and-is-present-in-biofilms
#11
Jaqueline López-Ochoa, J Fernando Montes-García, Candelario Vázquez, Patricia Sánchez-Alonso, Victor M Pérez-Márquez, Patrick J Blackall, Sergio Vaca, Erasmo Negrete-Abascal
Gallibacterium, which is a bacterial pathogen in chickens, can form biofilms. Amyloid proteins present in biofilms bind Congo red dye. The aim of this study was to characterize the cell-surface amyloid-like protein expressed in biofilms formed by Gallibacterium strains and determine the relationship between this protein and curli, which is an amyloid protein that is commonly expressed by members of the Enterobacteriaceae family. The presence of amyloid-like proteins in outer membrane protein samples from three strains of G...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28864971/proteomics-and-1-h-nmr-based-metabolomics-analysis-of-pathogenic-vibrio-vulnificus-aquacultures-isolated-from-sewage-drains
#12
Chundan Zhang, Zhonghua Wang, Dijun Zhang, Jun Zhou, Chenyang Lu, Xiurong Su, Dewen Ding
Vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. Vibrio vulnificus is a pathogenic bacterium that infects people and livestock. It is usually found in offshore waters or within fish and shellfish. This study presents a comparative proteomic analysis of the outer membrane protein (OMP) changes in V. vulnificus proteins after stimulation with sewage from sewage drains. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 32 protein spots with significant differences in abundance were identified and characterized...
September 1, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28861396/the-role-of-ompr-in-the-expression-of-genes-of-the-kdgr-regulon-involved-in-the-uptake-and-depolymerization-of-oligogalacturonides-in-yersinia-enterocolitica
#13
Marta Nieckarz, Adrianna Raczkowska, Karolina Jaworska, Ewa Stefańska, Karolina Skorek, Dorota Stosio, Katarzyna Brzostek
Oligogalacturonide (OGA)-specific porins of the KdgM family have previously been identified and characterized in enterobacterial plant pathogens. We found that deletion of the gene encoding response regulator OmpR causes the porin KdgM2 to become one of the most abundant proteins in the outer membrane of the human enteropathogen Yersinia enterocolitica. Reporter gene fusion and real-time PCR analysis confirmed that the expression of kdgM2 is repressed by OmpR. We also found that kdgM2 expression is subject to negative regulation by KdgR, a specific repressor of genes involved in the uptake and metabolism of pectin derivatives in plant pathogens...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28846684/outer-membrane-proteins-analysis-of-shigella-sonnei-and-evaluation-of-their-antigenicity-in-shigella-infected-individuals
#14
Hemavathy Harikrishnan, Kirnpal Kaur Banga Singh, Asma Ismail
Bacillary dysentery caused by infection with Shigella spp. remains as serious and common health problem throughout the world. It is a highly multi drug resistant organism and rarely identified from the patient at the early stage of infection. S. sonnei is the most frequently isolated species causing shigellosis in industrialized countries. The antigenicity of outer membrane protein of this pathogen expressed during human infection has not been identified to date. We have studied the antigenic outer membrane proteins expressed by S...
2017: PloS One
https://www.readbyqxmd.com/read/28844217/helicobacter-pylori-outer-membrane-protein-q-genotypes-and-their-susceptibility-to-anti-adhesive-phytotherapeutic-agents
#15
Javed Yakoob, Zaigham Abbas, Malik Hassan Mehmood, Kanwal Tariq, Saima Azhar Saleem, Safia Awan, Abdul Malik, Saeed Hamid, Rustam Khan, Wasim Jafri
OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q (HopQ) mediates host-pathogen interactions; HopQ genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against HopQ genotypes and H. pylori cytotoxin-associated gene A (CagA). METHODS: DNA was extracted by polymerase chain reaction of the HopQ genotypes (i...
September 2017: Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28835498/structural-transitions-of-the-conserved-and-metastable-hantaviral-glycoprotein-envelope
#16
Ilona Rissanen, Robert Stass, Antra Zeltina, Sai Li, Jussi Hepojoki, Karl Harlos, Robert J C Gilbert, Juha T Huiskonen, Thomas A Bowden
Hantaviruses are zoonotic pathogens with a near-global distribution that can cause severe hemorrhagic fever and pulmonary syndrome. The outer membrane of the hantavirus envelope displays a lattice of two glycoproteins, Gn and Gc, which orchestrate host cell recognition and entry. Here, we describe the crystal structure of the Gn glycoprotein ectodomain from the Asiatic Hantaan virus (HTNV), the most prevalent pathogenic hantavirus. Structural overlay analysis reveals that the HTNV Gn fold is highly similar to the Gn of Puumala virus (PUUV), a genetically and geographically distinct and less pathogenic hantavirus found predominantly in North-Eastern Europe, confirming that the hantaviral Gn fold is architecturally conserved across hantavirus clades...
August 23, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28823106/mitochondrial-outer-membrane-permeabilization-a-focus-on-the-role-of-mitochondrial-membrane-structural-organization
#17
REVIEW
Siti Haji Suhaili, Hamed Karimian, Matthew Stellato, Tzong-Hsien Lee, Marie-Isabel Aguilar
Apoptosis is important in regulating cell death turnover and is mediated by the intrinsic and death receptor-based extrinsic pathways which converge at the mitochondrial outer membrane (MOM) leading to mitochondrial outer membrane permeabilization (MOMP). MOMP results in the release of apoptotic proteins that further activate the downstream pathway of apoptosis. Thus, tight regulation of MOMP is crucial in controlling apoptosis, and a lack of control may lead to tissue and organ malformation and the development of cancers...
August 19, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28821836/porv-is-an-outer-membrane-shuttle-protein-for-the-type-ix-secretion-system
#18
Michelle D Glew, Paul D Veith, Dina Chen, Dhana G Gorasia, Ben Peng, Eric C Reynolds
Porphyromonas gingivalis is a keystone pathogen associated with chronic periodontitis. Major virulence factors named gingipains (cysteine proteinases, RgpA, RgpB and Kgp) are secreted via the Type IX Secretion System (T9SS). These, together with approximately 30 other proteins, are secreted to the cell surface and anchored to the outer membrane by covalent modification to anionic lipopolysaccharide (A-LPS) via the novel Gram negative sortase, PorU. PorU is localised on the cell surface and cleaves the C-terminal domain signal (CTD) of T9SS substrates and conjugates their new C-termini to A-LPS...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821590/three-dimensional-analysis-of-chloroplast-structures-associated-with-virus-infection
#19
Xuejiao Jin, Zhihao Jiang, Kun Zhang, Pengfei Wang, Xiuling Cao, Ning Yue, Xueting Wang, Xuan Zhang, Yunqin Li, Dawei Li, Byung-Ho Kang, Yongliang Zhang
Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA [(+)RNA] virus, Barley stripe mosaic virus (BSMV) to observe chloroplast structural changes during infection by electron tomography (ET). The analyses revealed remodeling of the chloroplast membranes, characterized by the clustering of outer membrane-invaginated spherules in inner membrane-derived packets...
August 18, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28819924/dynamic-imaging-of-mitochondrial-membrane-proteins-in-specific-sub-organelle-membrane-locations
#20
REVIEW
Timo Appelhans, Karin B Busch
Mitochondria are cellular organelles with multifaceted tasks and thus composed of different sub-compartments. The inner mitochondrial membrane especially has a complex nano-architecture with cristae protruding into the matrix. Related to their function, the localization of mitochondrial membrane proteins is more or less restricted to specific sub-compartments. In contrast, it can be assumed that membrane proteins per se diffuse unimpeded through continuous membranes. Fluorescence recovery after photobleaching is a versatile technology used in mobility analyses to determine the mobile fraction of proteins, but it cannot provide data on subpopulations or on confined diffusion behavior...
August 17, 2017: Biophysical Reviews
keyword
keyword
119647
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"