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outer membrane protein analysis

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https://www.readbyqxmd.com/read/28434130/characterization-of-extracellular-membrane-vesicles-of-an-antarctic-bacterium-shewanella-livingstonensis-ac10-and-their-enhanced-production-by-alteration-of-phospholipid-composition
#1
Fumiaki Yokoyama, Jun Kawamoto, Tomoya Imai, Tatsuo Kurihara
A cold-adapted bacterium, Shewanella livingstonensis Ac10, which produces eicosapentaenoic acid (EPA) as a component of its membrane phospholipids, is useful as a model to study the function of EPA and as a host for heterologous production of thermolabile proteins at low temperatures. In this study, we characterized extracellular membrane vesicles (EMVs) of this bacterium to examine the involvement of EPA in the biogenesis of EMVs and for the future application of EMVs to extracellular protein production. We found that this strain produced EMVs from the cell surface...
April 22, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28433003/the-plastid-outer-membrane-localized-lptd1-is-important-for-glycerolipid-remodeling-under-phosphate-starvation
#2
Yi-Ching Hsueh, Christian Ehmann, Nadine Flinner, Roman Ladig, Enrico Schleiff
Glycerolipid synthesis in plants is coordinated between plastids and the endoplasmic reticulum (ER). A central step within the glycerolipid synthesis is the transport of phosphatidic acid from ER to chloroplasts. The chloroplast outer envelope protein TGD4 belongs to the LptD family conserved in bacteria and plants and selectively binds and may transports phosphatidic acid. We describe a second LptD-family protein in A. thaliana (atLPTD1; At2g44640) characterized by a barrel domain with an amino acid signature typical for cyanobacterial LptDs...
April 22, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28431403/association-of-the-vdac3-gene-polymorphism-with-sperm-count-in-han-chinese-population-with-idiopathic-male-infertility
#3
Lianjun Pan, Qingzhen Liu, Jingyun Li, Wei Wu, Xinru Wang, Dan Zhao, Jiehua Ma
Voltage-dependent anion channel (VDAC) is a multifunctional channel protein across the outer mitochondrial membrane of somatic cells and participates in many physiological and pathophysiological processes. Up to now, only a few studies, including our previous studies, showed that VDAC exists in mammalian spermatozoa and is involved in spermatogenesis and sperm functions. There is no report about VDAC genetic variants in germinal tissues or cells. To investigate the possible association between VDAC genetic variants and human sperm quality, we performed semen analysis and variant Genotyping of VDAC3 subtype (rs7004637, rs16891278 and rs6773) of 523 Han-Chinese males with idiopathic infertility respectively by computer assisted semen analysis (CASA) and single nucleotide polymorphism (SNP) Genotyping assay...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421815/cross-protection-conferred-by-immunization-with-a-romph-based-intranasal-fowl-cholera-vaccine
#4
Thanya Varinrak, Pichayanut Poolperm, Takuo Sawada, Nattawooti Sthitmatee
A previous study demonstrated that a recombinant outer membrane protein H (rOmpH)-based intranasal fowl cholera vaccine elicited efficient homologous protection against Pasteurella multocida strain X-73 (A:1) in chickens. The present study aimed to determine the cross protectivity against heterologous P. multocida strains. The rOmpH was purified via electroelution and formulated with two kinds of adjuvants. The vaccine formulations in a total volume of 100 µl were 100 µg rOmpH with 3 µg of Escherichia coli enterotoxin B (LTB) or 10 µg of CpG ODN2007...
April 19, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28420794/structural-features-and-lipid-binding-domain-of-tubulin-on-biomimetic-mitochondrial-membranes
#5
David P Hoogerheide, Sergei Y Noskov, Daniel Jacobs, Lucie Bergdoll, Vitalii Silin, David L Worcester, Jeff Abramson, Hirsh Nanda, Tatiana K Rostovtseva, Sergey M Bezrukov
Dimeric tubulin, an abundant water-soluble cytosolic protein known primarily for its role in the cytoskeleton, is routinely found to be associated with mitochondrial outer membranes, although the structure and physiological role of mitochondria-bound tubulin are still unknown. There is also no consensus on whether tubulin is a peripheral membrane protein or is integrated into the outer mitochondrial membrane. Here the results of five independent techniques-surface plasmon resonance, electrochemical impedance spectroscopy, bilayer overtone analysis, neutron reflectometry, and molecular dynamics simulations-suggest that α-tubulin's amphipathic helix H10 is responsible for peripheral binding of dimeric tubulin to biomimetic "mitochondrial" membranes in a manner that differentiates between the two primary lipid headgroups found in mitochondrial membranes, phosphatidylethanolamine and phosphatidylcholine...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28413119/the-two-dictyostelium-discoideum-autophagy-8-proteins-have-distinct-autophagic-functions
#6
Susanne Meßling, Jan Matthias, Qiuhong Xiong, Sarah Fischer, Ludwig Eichinger
Autophagy is a highly conserved cellular degradation pathway which is crucial for various cellular processes. The autophagic process is subdivided in the initiation, autophagosome maturation and lysosomal degradation phases and involves more than forty core and accessory autophagy-related (ATG) proteins. Autophagy 8 (ATG8, in mammals LC3) is a well-established marker of autophagy and is linked to the autophagic membrane from initiation until fusion with the lysosome. We generated single and double knock-out mutants of the two Dictyostelium paralogues, ATG8a and ATG8b, as well as strains that expressed RFP-ATG8a and/or GFP-ATG8b, RFP-ATG8b, RFP-GFP-ATG8a or RFP-GFP-ATG8b in different knock-out mutants...
April 4, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28406958/molecular-analysis-of-culex-quinquefasciatus-larvae-responses-to-lysinibacillus-sphaericus-bin-toxin
#7
Chontida Tangsongcharoen, Natapong Jupatanakul, Boonhiang Promdonkoy, George Dimopoulos, Panadda Boonserm
Lysinibacillus sphaericus produces the mosquito larvicidal binary toxin consisting of BinA and BinB, which are both required for toxicity against Culex and Anopheles larvae. The molecular mechanisms behind Bin toxin-induced damage remain unexplored. We used whole-genome microarray-based transcriptome analysis to better understand how Culex larvae respond to Bin toxin treatment at the molecular level. Our analyses of Culex quinquefasciatus larvae transcriptome changes at 6, 12, and 18 h after Bin toxin treatment revealed a wide range of transcript signatures, including genes linked to the cytoskeleton, metabolism, immunity, and cellular stress, with a greater number of down-regulated genes than up-regulated genes...
2017: PloS One
https://www.readbyqxmd.com/read/28394313/structure-of-the-mycobacterial-esx-5-type-vii-secretion-system-membrane-complex-by-single-particle-analysis
#8
Katherine S H Beckham, Luciano Ciccarelli, Catalin M Bunduc, Haydyn D T Mertens, Roy Ummels, Wolfgang Lugmayr, Julia Mayr, Mandy Rettel, Mikhail M Savitski, Dmitri I Svergun, Wilbert Bitter, Matthias Wilmanns, Thomas C Marlovits, Annabel H A Parret, Edith N G Houben
Mycobacteria are characterized by their impermeable outer membrane, which is rich in mycolic acids(1). To transport substrates across this complex cell envelope, mycobacteria rely on type VII (also known as ESX) secretion systems(2). In Mycobacterium tuberculosis, these ESX systems are essential for growth and full virulence and therefore represent an attractive target for anti-tuberculosis drugs(3). However, the molecular details underlying type VII secretion are largely unknown, due to a lack of structural information...
April 10, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28393831/determining-the-bacterial-cell-biology-of-planctomycetes
#9
Christian Boedeker, Margarete Schüler, Greta Reintjes, Olga Jeske, Muriel C F van Teeseling, Mareike Jogler, Patrick Rast, Daniela Borchert, Damien P Devos, Martin Kucklick, Miroslava Schaffer, Roberto Kolter, Laura van Niftrik, Susanne Engelmann, Rudolf Amann, Manfred Rohde, Harald Engelhardt, Christian Jogler
Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail...
April 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28377068/solution-structure-and-dynamics-of-the-outer-membrane-cytochrome-omcf-from-geobacter-sulfurreducens
#10
Joana M Dantas, Marta A Silva, David Pantoja-Uceda, David L Turner, Marta Bruix, Carlos A Salgueiro
Gene knock-out studies on Geobacter sulfurreducens cells showed that the outer membrane-associated monoheme cytochrome OmcF is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI). In addition, microarray analysis of an OmcF-deficient mutant revealed that many of the genes with decreased transcript level were those whose expression is up-regulated in cells grown with a graphite electrode as electron acceptor, suggesting that OmcF also regulates the electron transfer to electrode surfaces and the concomitant electricity production by G...
April 1, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28376086/novel-insights-into-slc25a46-related-pathologies-in-a-genetic-mouse-model
#11
Maria Eirini Terzenidou, Aikaterini Segklia, Toshimi Kano, Florentia Papastefanaki, Alexandros Karakostas, Maria Charalambous, Fotis Ioakeimidis, Maria Papadaki, Ismini Kloukina, Margarita Chrysanthou-Piterou, Martina Samiotaki, George Panayotou, Rebecca Matsas, Eleni Douni
The mitochondrial protein SLC25A46 has been recently identified as a novel pathogenic cause in a wide spectrum of neurological diseases, including inherited optic atrophy, Charcot-Marie-Tooth type 2, Leigh syndrome, progressive myoclonic ataxia and lethal congenital pontocerebellar hypoplasia. SLC25A46 is an outer membrane protein, member of the Solute Carrier 25 (SLC25) family of nuclear genes encoding mitochondrial carriers, with a role in mitochondrial dynamics and cristae maintenance. Here we identified a loss-of-function mutation in the Slc25a46 gene that causes lethal neuropathology in mice...
April 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28373551/identification-of-specific-posttranslational-o-mycoloylations-mediating-protein-targeting-to-the-mycomembrane
#12
Clément Carel, Julien Marcoux, Valérie Réat, Julien Parra, Guillaume Latgé, Françoise Laval, Pascal Demange, Odile Burlet-Schiltz, Alain Milon, Mamadou Daffé, Maryelle G Tropis, Marie A M Renault
The outer membranes (OMs) of members of the Corynebacteriales bacterial order, also called mycomembranes, harbor mycolic acids and unusual outer membrane proteins (OMPs), including those with α-helical structure. The signals that allow precursors of such proteins to be targeted to the mycomembrane remain uncharacterized. We report here the molecular features responsible for OMP targeting to the mycomembrane of Corynebacterium glutamicum, a nonpathogenic member of the Corynebacteriales order. To better understand the mechanisms by which OMP precursors were sorted in C...
April 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28369099/comparative-gene-expression-analysis-of-porphyromonas-gingivalis-atcc-33277-in-planktonic-and-biofilms-states
#13
P Romero-Lastra, M C Sánchez, H Ribeiro-Vidal, A Llama-Palacios, E Figuero, D Herrera, M Sanz
BACKGROUND AND OBJECTIVE: Porphyromonas gingivalis is a keystone pathogen in the onset and progression of periodontitis. Its pathogenicity has been related to its presence and survival within the subgingival biofilm. The aim of the present study was to compare the genome-wide transcription activities of P. gingivalis in biofilm and in planktonic growth, using microarray technology. MATERIAL AND METHODS: P. gingivalis ATCC 33277 was incubated in multi-well culture plates at 37°C for 96 hours under anaerobic conditions using an in vitro static model to develop both the planktonic and biofilm states (the latter over sterile ceramic calcium hydroxyapatite discs)...
2017: PloS One
https://www.readbyqxmd.com/read/28368278/crystallization-and-x-ray-analysis-of-the-extracellular-adhesion-domain-of-helicobacter-pylori-adhesin-a-the-significance-of-the-cation-composition-in-the-crystallization-precipitant
#14
Ling Guo, Jinyong Zhang, Liwei Cui, Dong Liu, Bo Ma, Shufeng Wang, Haibo Li, Yuzhang Wu, Wei Liu
Adherence to host cells is a crucial step in the process of bacterial infection, which is usually mediated by a number of outer membrane proteins identified as adhesins. Helicobacter pylori adhesin A (HpaA) is a member of the adhesin family that mediates the adherence of Helicobacter pylori to gastric epithelial cells, and consequently assists the bacteria in becoming a life-long colonizer of the human stomach. In this study, two constructs were made for the production of truncated HpaA proteins comprising residues 31-260 and 53-260, respectively...
April 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28362919/activation-of-nuclear-factor-kappa-b-in-endothelial-cells-of-chronic-subdural-hematoma-outer-membranes
#15
Koji Osuka, Yasuo Watanabe, Nobuteru Usuda, Masahiro Aoyama, Reo Kawaguchi, Mikinobu Takeuchi, Masakazu Takayasu
BACKGROUND: Chronic subdural hematoma (CSDH) is considered an angiogenic and inflammatory disease. Nuclear factor-kappa B (NF-κB) induces the production of inflammatory cytokines and adhesion molecules, which play an essential role in angiogenesis and inflammation. Recently, the double-stranded RNA-activated protein kinase (PKR) was shown to directly interact with NF-κB subunits to influence its transcriptional activity. OBJECTIVE: To examine the expression of NF-κB signaling pathway components and PKR in CSDH outer membranes...
April 1, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28356353/a-ternary-membrane-protein-complex-anchors-the-spindle-pole-body-in-the-nuclear-envelope-in-budding-yeast
#16
Thomas Kupke, Jörg Malsam, Elmar Schiebel
In budding yeast (Saccharomyces cerevisiae) the multi-layered spindle pole body (SPB) is embedded in the nuclear envelope (NE) at fusion sites of the inner and outer nuclear membrane. The SPB is built from 18 different proteins, including the three integral membrane proteins Mps3, Ndc1, and Mps2. These membrane proteins play an essential role in the insertion of the new SPB into the NE. How the huge core structure of the SPB is anchored in the NE has not been investigated thoroughly until now. The present model suggests that the NE protein Mps2 interacts via Bbp1 with Spc29, one of the coiled-coil proteins forming the central plaque of the SPB...
March 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28352257/a-novel-pan-genome-reverse-vaccinology-approach-employing-a-negative-selection-strategy-for-screening-surface-exposed-antigens-against-leptospirosis
#17
LingBing Zeng, Dongliang Wang, NiYa Hu, Qing Zhu, Kaishen Chen, Ke Dong, Yan Zhang, YuFeng Yao, XiaoKui Guo, Yung-Fu Chang, YongZhang Zhu
Reverse vaccinology (RV) has been widely used for screening of surface-exposed proteins (PSEs) of important pathogens, including outer membrane proteins (OMPs), and extracellular proteins (ECPs) as potential vaccine candidates. In this study, we applied a novel RV negative strategy and a pan-genome analysis for screening of PSEs from 17 L. interrogans strains covering 11 predominately epidemic serovars and 17 multilocus typing (MLST) sequence types (STs) worldwide. Our results showed, for instance, out of a total of 633 predicted PSEs in strain 56601, 92...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28341438/engineering-a-novel-porin-ompgf-via-strand-replacement-from-computational-analysis-of-sequence-motif
#18
Meishan Lin, Ge Zhang, Monifa Fahie, Leslie K Morgan, Min Chen, Timothy A Keiderling, Linda J Kenney, Jie Liang
β-Barrelmembrane proteins (βMPs) form barrel-shaped pores in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts. Because of the robustness of their barrel structures, βMPs have great potential as nanosensors for single-molecule detection. However, natural βMPs currently employed have inflexible biophysical properties and are limited in their pore geometry, hindering their applications in sensing molecules of different sizes and properties. Computational engineering has the promise to generate βMPs with desired properties...
March 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28333095/cytosolic-bnip3-dimer-interacts-with-mitochondrial-bax-forming-heterodimers-in-the-mitochondrial-outer-membrane-under-basal-conditions
#19
Ulrike B Hendgen-Cotta, Sonja Esfeld, Katharina Rudi, Ilkka Miinalainen, Johann P Klare, Tienush Rassaf
The primary function of mitochondria is energy production, a task of particular importance especially for cells with a high energy demand like cardiomyocytes. The B-cell lymphoma (BCL-2) family member BCL-2 adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) is linked to mitochondrial targeting after homodimerization, where it functions in inner membrane depolarization and permeabilization of the mitochondrial outer membrane (MOM) mediating cell death. We investigated the basal distribution of cardiac BNIP3 in vivo and its physical interaction with the pro-death protein BCL2 associated X, apoptosis regulator (BAX) and with mitochondria using immunoblot analysis, co-immunoprecipitation, and continuous wave and pulsed electron paramagnetic resonance spectroscopy techniques...
March 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28331079/characterization-and-vaccine-potential-of-membrane-vesicles-produced-by-francisella-noatunensis-sup-orientalis-in-an-adult-zebrafish-model
#20
Leidy Lagos, Julia I Tandberg, Urska Repnik, Preben Boysen, Erik Ropstad, Deepa Varkey, Ian T Paulsen, Hanne C Winther-Larsen
Vaccine development against extracellular bacteria has been important for the sustainability of the aquaculture industry. In contrast, infections with intracellular pathogens remain largely an unresolved problem. Francisella noatunensis subspecies orientalis (Fno) are Gram-negative, facultative intracellular bacteria that cause the disease francisellosis in fish. Francisellosis is commonly characterized as a chronic granulomatous disease with high morbidity and can result in high mortality depending on the host...
March 22, 2017: Clinical and Vaccine Immunology: CVI
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