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outer membrane protein analysis

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https://www.readbyqxmd.com/read/27920824/cloning-expression-and-molecular-analysis-of-iranian-brucella-melitensis-omp25-gene-for-designing-a-subunit-vaccine
#1
Soheil Yousefi, Mojtaba Tahmoorespur, Mohammad Hadi Sekhavati
Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. The outer membrane protein 25 kDa (Omp25) gene plays an important role in simulating of TNF-α, IFN-α, macrophage, and cytokines cells. In the current study molecular cloning and expression analysis of Omp25 gene for designing a subunit vaccine against Brucella was investigated. Amplifying the full length of candidate gene was performed using specific primers. Sub-cloning of this gene conducted using pTZ57R/T vector in TOP10F strain of Escherichia coli(E...
October 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27920758/effectiveness-of-f18-fimbrial-antigens-released-by-a-novel-autolyzed-salmonella-expression-system-as-a-vaccine-candidate-against-lethal-f18-stec-infection
#2
Gayeon Won, John H Lee
Porcine edema disease (ED) caused by Shiga toxin 2e producing Escherichia coli expressing F18ab(+) fimbriae (F18ab(+)STEC) frequently occurs in post-weaned piglets, resulting in a significant economic loss in swine industries worldwide. In the present study, we proposed an efficient prevention scheme against ED in which the attenuated Salmonella Typhimurium inactivated by the E-mediated cell lysis to deliver target antigens, FedF and FedA, which function in fimbrial-mediated adhesion and as a major subunit of F18ab+fimbriae, respectively...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27915254/ompf-of-pectobacterium-carotovorum-subsp-carotovorum-pcc3-is-required-for-carocin-d-sensitivity
#3
Jeong-A Lim, Jisoo Hong, Jonguk Kim, Sunggi Heu, Eunjung Roh
Carocin D is a bacteriocin produced by Pectobacterium carotovorum subsp. carotovorum Pcc21. Carocin D inhibits the growth of P carotovorum subsp. carotovorum and closely related strains, P carotovorum subsp. carotovorum is causative bacteria for soft rot disease and leads to severe economic losses. Bacteriocins recognize and interact with a specific membrane protein of target bacteria as a receptor. To identify the receptor responsible for carocin D recognition, mutants that underwent a phenotypic change from carocin D-sensitivity to carocin D-insensitivity were screened...
December 2, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27907096/global-deletion-of-tspo-does-not-affect-the-viability-and-gene-expression-profile
#4
Huaishan Wang, Kangle Zhai, Yingchao Xue, Jia Yang, Qi Yang, Yi Fu, Yu Hu, Fang Liu, Weiqing Wang, Lianxian Cui, Hui Chen, Jianmin Zhang, Wei He
Translocator Protein (18kDa, TSPO) is a mitochondrial outer membrane transmembrane protein. Its expression is elevated during inflammation and injury. However, the function of TSPO in vivo is still controversial. Here, we constructed a TSPO global knockout (KO) mouse with a Cre-LoxP system that abolished TSPO protein expression in all tissues and showed normal phenotypes in the physiological condition. The birth rates of TSPO heterozygote (Het) x Het or KO x KO breeding were consistent with Mendel's Law, suggesting a normal viability of TSPO KO mice at birth...
2016: PloS One
https://www.readbyqxmd.com/read/27893179/ctra-controls-cell-division-and-outer-membrane-composition-of-the-pathogen-brucella-abortus
#5
Nayla Francis, Katy Poncin, Antonella Fioravanti, Victoria Vassen, Kevin Willemart, Thi Anh Phuong Ong, Luca Rappez, Jean-Jacques Letesson, Emanuele G Biondi, Xavier De Bolle
Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide...
November 28, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27888458/helicobacter-pylori-outer-membrane-protein-homc-shows-geographic-dependent-polymorphism-that-is-influenced-by-the-bab-family
#6
Aeryun Kim, Stephanie L Servetas, Jieun Kang, Jinmoon Kim, Sungil Jang, Yun Hui Choi, Hanfu Su, Yeong-Eui Jeon, Youngmin A Hong, Yun-Jung Yoo, D Scott Merrell, Jeong-Heon Cha
The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27884170/first-report-of-rickettsia-raoultii-and-r-slovaca-in-melophagus-ovinus-the-sheep-ked
#7
Dan Liu, Yuan-Zhi Wang, Huan Zhang, Zhi-Qiang Liu, Ha-Zi Wureli, Shi-Wei Wang, Chang-Chun Tu, Chuang-Fu Chen
BACKGROUND: Melophagus ovinus (Diptera: Hippoboscidae), a hematophagous ectoparasite, is mainly found in Europe, Northwestern Africa, and Asia. This wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. Furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. Herein, we investigated the presence of various Rickettsia species in M. ovinus. METHODS: In this study, a total of 95 sheep keds were collected in Kuqa County and Alaer City southern region of Xinjiang Uygur Autonomous Region, northwestern China...
November 25, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27882088/study-on-the-resistance-mechanism-via-outer-membrane-protein-oprd2-and-metal-%C3%AE-lactamase-expression-in-the-cell-wall-of-pseudomonas-aeruginosa
#8
Shuangqi Cai, Yiqiang Chen, Dezhi Song, Jinliang Kong, Yanbin Wu, Huasong Lu
The aim of the present study was to evaluate the imipenem-resistant mechanism via the outer membrane protein (OMP) OprD2 and metal β-lactamase expression in the cell wall of Pseudomonas aeruginosa. The Pseudomonas aeruginosa was clinically separated and validated by VITEK-2 full-automatic bacteria analyzer. Drug resistance, sensitive antibiotics and minimum inhibitory concentration (MIC) were tested using the drug sensitivity analysis system. The phenotype positive strains of MBL genes were screened using the Kirby-Bauer diffusion method by adding metal ion-chelating agent EDTA on the imipenem susceptibility paper...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27881906/genetic-and-immunohistochemical-analysis-of-hspa5-in-mouse-and-human-retinas
#9
Sumana R Chintalapudi, XiaoFei Wang, Huiling Li, Yin H Chan Lau, Robert W Williams, Monica M Jablonski
PURPOSE: Photoreceptor degenerative diseases are among the leading causes of vision loss. Although the causative genetic mutations are often known, mechanisms leading to photoreceptor degeneration remain poorly defined. We have previously demonstrated that the photoreceptor membrane-associated protein XAP-1 antigen is a product of the HSPA5 gene. In this study, we used systems genetic methods, statistical modeling, and immunostaining to identify and analyze candidate genes that modulate Hspa5 expression in the retina...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27878870/epidermolysis-bullosa-simplex-in-sibling-eurasier-dogs-is-caused-by-a-plec-non-sense-variant
#10
Elizabeth A Mauldin, Ping Wang, Thierry Olivry, Paula S Henthorn, Margret L Casal
BACKGROUND: Plectin, a large linker protein found in many tissues, acts to connect components of the cytoskeleton to each other. In the epidermis, plectin binds keratin intermediate filaments to hemidesmosomes. A deficiency of plectin in the skin leads to blister formation in the basal layer and the disease epidermolysis bullosa simplex (EBS). HYPOTHESIS/OBJECTIVES: To describe a novel blistering disease that arose spontaneously in a litter of puppies. ANIMALS: Two female and one male 20-day-old Eurasier puppies, from a litter of six, were presented for evaluation of failure to thrive and then euthanized due to poor prognosis...
November 7, 2016: Veterinary Dermatology
https://www.readbyqxmd.com/read/27872187/galactomannan-catabolism-conferred-by-a-polysaccharide-utilisation-locus-of-bacteroides-ovatus-enzyme-synergy-and-crystal-structure-of-a-%C3%AE-mannanase
#11
Viktoria Bågenholm, Sumitha K Reddy, Hanene Bouraoui, Johan Morrill, Evelina Kulcinskaja, Constance M Bahr, Oskar Aurelius, Theresa Rogers, Yao Xiao, Derek T Logan, Eric C Martens, Nicole M Koropatkin, Henrik Stålbrand
A recently identified polysaccharide utilisation locus (PUL) from Bacteroides ovatus ATCC8483 is transcriptionally upregulated during growth on galacto- and glucomannans. It encodes two putative glycoside hydrolase family 26 (GH26) β-mannanases, BoMan26A and BoMan26B, and a GH36 α-galactosidase, BoGal36A. The PUL also includes two glycan binding proteins, confirmed by β-mannan affinity electrophoresis. When this PUL was deleted, B. ovatus was no longer able to grow on locust bean galactomannan. BoMan26A primarily formed mannobiose from mannan polysaccharides...
November 21, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27867392/the-physcomitrella-patens-chloroplast-proteome-changes-in-response-to-protoplastation
#12
Igor Fesenko, Anna Seredina, Georgij Arapidi, Vasily Ptushenko, Anatoly Urban, Ivan Butenko, Sergey Kovalchuk, Konstantin Babalyan, Andrey Knyazev, Regina Khazigaleeva, Elena Pushkova, Nikolai Anikanov, Vadim Ivanov, Vadim M Govorun
Plant protoplasts are widely used for genetic manipulation and functional studies in transient expression systems. However, little is known about the molecular pathways involved in a cell response to the combined stress factors resulted from protoplast generation. Plants often face more than one type of stress at a time, and how plants respond to combined stress factors is therefore of great interest. Here, we used protoplasts of the moss Physcomitrella patens as a model to study the effects of short-term stress on the chloroplast proteome...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27866852/ompa-a-flexible-clamp-for-bacterial-cell-wall-attachment
#13
Firdaus Samsudin, Maite L Ortiz-Suarez, Thomas J Piggot, Peter J Bond, Syma Khalid
The envelope of Gram-negative bacteria is highly complex, containing separate outer and inner membranes and an intervening periplasmic space encompassing a peptidoglycan (PGN) cell wall. The PGN scaffold is anchored non-covalently to the outer membrane via globular OmpA-like domains of various proteins. We report atomically detailed simulations of PGN bound to OmpA in three different states, including the isolated C-terminal domain (CTD), the full-length monomer, or the complete full-length dimeric form. Comparative analysis of dynamics of OmpA CTD from different bacteria helped to identify a conserved PGN-binding mode...
November 8, 2016: Structure
https://www.readbyqxmd.com/read/27861601/the-development-of-drosophila-melanogaster-under-different-duration-space-flight-and-subsequent-adaptation-to-earth-gravity
#14
Irina V Ogneva, Stepan N Belyakin, Svetlana V Sarantseva
In prospective human exploration of outer space, the need to preserve a species over several generations under changed gravity conditions may arise. This paper demonstrates our results in the creation of the third generation of fruit fly Drosophila melanogaster (third-stage larvae) during the 44.5-day space flight (Foton-M4 satellite (2014, Russia)), then the fourth generation on Earth and the fifth generation again in conditions of the 12-day space flight (2014, in the Russian Segment of the ISS). The species preserves fertility despite a number of changes in the level of expression and content of cytoskeletal proteins, which are the key components of the cleavage spindle and the contractile ring of cells...
2016: PloS One
https://www.readbyqxmd.com/read/27860045/immunization-with-r-lactococcus-lactis-expressing-outer-membrane-protein-a-ompa-of-shigella-dysenteriae-type-1-evaluation-of-oral-and-intranasal-route-of-administration
#15
Bhrugu Yagnik, Drashya Sharma, Harish Padh, Priti Desai
AIMS: To evaluate the comparative immunogenic potential of food grade Lactococcus lactis (L. lactis) expressing Outer membrane protein A (OmpA) of Shigella dysenteriae type-1 (SD-1) when administered either orally or intranasally. METHODS AND RESULTS: OmpA of SD-1 was cloned and expressed first in Escherichia coli (E. coli) and then in L. lactis. Presence of recombinant gene was confirmed by restriction enzyme digestion and Immunoblot analysis. Using immobilized metal affinity chromatography (IMAC), OmpA was purified from recombinant E...
November 17, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27856491/excavating-the-surface-associated-and-secretory-proteome-of-mycobacterium-leprae-for-identifying-vaccines-and-diagnostic-markers-relevant-immunodominant-epitopes
#16
Aarti Rana, Shweta Thakur, Nupur Bhardwaj, Devender Kumar, Yusuf Akhter
For centuries Mycobacterium leprae, etiological agent of leprosy, has been afflicting mankind regardless of extensive use of live attenuated vaccines and antibiotics. Surface-associated and secretory proteins (SASPs) are attractive targets against bacteria. We have integrated biological knowledge with computational approaches and present a proteome-wide identification of SASPs. We also performed computational assignment of immunodominant epitopes as coordinates of prospective antigenic candidates in most important class of SASPs, the outer membrane proteins (OMPs)...
November 16, 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/27852378/-analysis-of-the-protein-expression-differences-between-colonized-and-infectious-multidrug-resistant-acinetobacter-baumannii
#17
W J Sui, M Wang, Y F Huang, X X Lu
Objective: To analyze the protein expression differences between colonized multidrug-resistant acinetobacter baumannii (MDR-AB) and infectious MDR-AB using comparative proteomics and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and to explore the molecular characteristics of MDR-AB infections. Methods: A total of 59 MDR-AB strains including 23 of bloodstream infections MDR-AB and 36 of nasal, pharyngeal or environmental colonization MDR-AB were collected from Beijing Tongren Hospital between January 2008 and December 2011...
November 8, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27848237/a-protein-nanopore-based-approach-for-bacteria-sensing
#18
Aurelia Apetrei, Andrei Ciuca, Jong-Kook Lee, Chang Ho Seo, Yoonkyung Park, Tudor Luchian
We present herein a first proof of concept demonstrating the potential of a protein nanopore-based technique for real-time detection of selected Gram-negative bacteria (Pseudomonas aeruginosa or Escherichia coli) at a concentration of 1.2 × 10(8) cfu/mL. The anionic charge on the bacterial outer membrane promotes the electrophoretically driven migration of bacteria towards a single α-hemolysin nanopore isolated in a lipid bilayer, clamped at a negative electric potential, and followed by capture at the nanopore's mouth, which we found to be described according to the classical Kramers' theory...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27834373/in-vivo-protein-interaction-network-analysis-reveals-porin-localized-antibiotic-inactivation-in-acinetobacter-baumannii-strain-ab5075
#19
Xia Wu, Juan D Chavez, Devin K Schweppe, Chunxiang Zheng, Chad R Weisbrod, Jimmy K Eng, Ananya Murali, Samuel A Lee, Elizabeth Ramage, Larry A Gallagher, Hemantha D Kulasekara, Mauna E Edrozo, Cassandra N Kamischke, Mitchell J Brittnacher, Samuel I Miller, Pradeep K Singh, Colin Manoil, James E Bruce
The nosocomial pathogen Acinetobacter baumannii is a frequent cause of hospital-acquired infections worldwide and is a challenge for treatment due to its evolved resistance to antibiotics, including carbapenems. Here, to gain insight on A. baumannii antibiotic resistance mechanisms, we analyse the protein interaction network of a multidrug-resistant A. baumannii clinical strain (AB5075). Using in vivo chemical cross-linking and mass spectrometry, we identify 2,068 non-redundant cross-linked peptide pairs containing 245 intra- and 398 inter-molecular interactions...
November 11, 2016: Nature Communications
https://www.readbyqxmd.com/read/27832917/immune-response-characterization-and-vaccine-potential-of-a-recombinant-chimera-comprising-b-cell-epitope-of-aeromonas-hydrophila-outer-membrane-protein-c-and-ltb
#20
Mahima Sharma, Aparna Dixit
Aeromonas hydrophila is one of the most virulent fish pathogens, causing colossal economic losses to the aquaculture industry annually. The absence of a safe and effective vaccine makes it very difficult to control this infection. Outer membrane proteins have been widely illustrated to confer protective immunity against a broad spectrum of gram negative bacteria. In the current study, we have analyzed the ability of B-cell epitopes of A. hydrophila's outer membrane protein C (OmpC) to confer protection against bacterial virulence...
December 7, 2016: Vaccine
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