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outer membrane protein analysis

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https://www.readbyqxmd.com/read/28716905/mitoneet-dependent-formation-of-intermitochondrial-junctions
#1
Alexandre Vernay, Anna Marchetti, Ayman Sabra, Tania N Jauslin, Manon Rosselin, Philipp E Scherer, Nicolas Demaurex, Lelio Orci, Pierre Cosson
MitoNEET (mNEET) is a dimeric mitochondrial outer membrane protein implicated in many facets of human pathophysiology, notably diabetes and cancer, but its molecular function remains poorly characterized. In this study, we generated and analyzed mNEET KO cells and found that in these cells the mitochondrial network was disturbed. Analysis of 3D-EM reconstructions and of thin sections revealed that genetic inactivation of mNEET did not affect the size of mitochondria but that the frequency of intermitochondrial junctions was reduced...
July 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28713344/outer-membrane-proteome-of-veillonella-parvula-a-diderm-firmicute-of-the-human-microbiome
#2
Daniel I Poppleton, Magalie Duchateau, Véronique Hourdel, Mariette Matondo, Jennifer Flechsler, Andreas Klingl, Christophe Beloin, Simonetta Gribaldo
Veillonella parvula is a biofilm-forming commensal found in the lungs, vagina, mouth, and gastro-intestinal tract of humans, yet it may develop into an opportunistic pathogen. Furthermore, the presence of Veillonella has been associated with the development of a healthy immune system in infants. Veillonella belongs to the Negativicutes, a diverse clade of bacteria that represent an evolutionary enigma: they phylogenetically belong to Gram-positive (monoderm) Firmicutes yet maintain an outer membrane (OM) with lipopolysaccharide similar to classic Gram-negative (diderm) bacteria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28708841/nlpd-links-cell-wall-remodeling-and-outer-membrane-invagination-during-cytokinesis-in-escherichia-coli
#3
Mary-Jane Tsang, Anastasiya A Yakhnina, Thomas G Bernhardt
Cytokinesis in gram-negative bacteria requires the constriction of all three cell envelope layers: the inner membrane (IM), the peptidoglycan (PG) cell wall and the outer membrane (OM). In order to avoid potentially lethal breaches in cell integrity, this dramatic reshaping of the cell surface requires tight coordination of the different envelope remodeling activities of the cytokinetic ring. However, the mechanisms responsible for this coordination remain poorly defined. One of the few characterized regulatory points in the envelope remodeling process is the activation of cell wall hydrolytic enzymes called amidases...
July 14, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28708586/whole-genome-and-transcriptome-analysis-reveal-maldi-tof-ms-and-sds-page-have-limited-performance-for-the-detection-of-the-key-outer-membrane-protein-in-carbapenem-resistant-klebsiella-pneumoniae-isolates
#4
Naina Adren Pinto, Roshan D'Souza, In Sik Hwang, Jongrak Choi, Yong Ha In, Hyung Soon Park, Choong-Min Ryu, Dongeun Yong, Kyungwon Lee
To detect the outer membrane protein (OMP), which plays a key role in carbapenem resistance, whole-genome and transcriptome analysis of the clinical carbapenem-resistant Klebsiella pneumoniae was carried out. The index strain lacked both OmpK35 and OmpK36, whereas the other strains lacked only OmpK35. After SDS-PAGE, the putative OMP bands were excised and identified as OmpA and OmpK36. MALDI-TOF MS showed peaks at ~36 and ~38 kDa that corresponded to OmpA and OmpK36, respectively. In all the strains except YMC2014/03/P345, the ~38 kDa peaks were present...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28707473/aggregatibacter-actinomycetemcomitans-growth-in-biofilm-versus-planktonic-state-differential-expression-of-proteins
#5
Arancha Llama-Palacios, Oksana Potupa, María Del Carmen Sánchez, Elena Figuero, David Herrera, Mariano Sanz
Aggregatibacter actinomycetemcomitans (Aa) is a pathogenic bacterium residing in the subgingival plaque biofilm strongly associated with the pathogenesis of periodontitis. The aim of this investigation was to study the protein differential expression of Aa when growing on biofilm compared to planktonic state using proteomic analysis by the 2D-DIGE™ system. Eighty-seven proteins were differentially expressed during biofilm growth (1.5-fold, p<0.05), being 13 over expressed and 37 down expressed. Those repressed were mainly proteins involved in metabolism, biosynthesis and transport...
July 14, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28707374/localization-and-functional-analysis-of-the-insect-specific-rabx4-in-the-brain-of-bombyx-mori
#6
Tomohide Uno, Masayuki Furutani, Katsuhiko Sakamoto, Yuichi Uno, Kengo Kanamaru, Akira Mizoguchi, Susumu Hiragaki, Makio Takeda
Rab proteins are small monomeric GTPases/GTP-binding proteins, which form the largest branch of the Ras superfamily. The different Rab GTPases are localized to the cytosolic face of specific intracellular membranes, where they function as regulators of distinct steps in membrane trafficking. RabX4 is an insect-specific Rab protein that has no close homolog in vertebrates. There is little information about insect-specific Rab proteins. RabX4 was expressed in Escherichia coli and subsequently purified. Antibodies against Bombyx mori RabX4 were produced in rabbits for western immunoblotting and immunohistochemistry...
July 14, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28704394/genome-wide-host-gene-expression-analysis-in-mice-experimentally-infected-with-pasteurella-multocida
#7
G Bhuvana Priya, Viswas Konasagara Nagaleekar, A Arun Prince Milton, M Saminathan, Amod Kumar, Amit Ranjan Sahoo, Sajad Ahmad Wani, Amit Kumar, S K Gupta, Aditya P Sahoo, A K Tiwari, R K Agarwal, Ravi Kumar Gandham
Pasteurella multocida causes acute septicemic and respiratory diseases, including haemorrhagic septicaemia, in cattle and buffalo with case fatality of 100%. In the present study, mice were infected with P. multocida (1.6 × 103 cfu, intraperitoneal) to evaluate host gene expression profile at early and late stages of infection using high throughput microarray transcriptome analyses. Several differentially expressed genes (DEGs) at both the time points were identified in P.multocida infected spleen, liver and lungs...
2017: PloS One
https://www.readbyqxmd.com/read/28700912/quantitative-brightness-analysis-of-protein-oligomerization-in-the-nuclear-envelope
#8
Jared Hennen, Kwang-Ho Hur, Cosmo A Saunders, G W Gant Luxton, Joachim D Mueller
Brightness analysis of fluorescence fluctuation experiments has been used to successfully measure the oligomeric state of proteins at the plasma membrane, in the nucleoplasm, and in the cytoplasm of living cells. Here we extend brightness analysis to the nuclear envelope (NE), a double membrane barrier separating the cytoplasm from the nucleoplasm. Results obtained by applying conventional brightness analysis to fluorescently tagged proteins within the NE exhibited an unusual concentration dependence. Similarly, the autocorrelation function of the fluorescence fluctuations exhibited unexpected changes with protein concentration...
July 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28696348/relationship-of-triamine-biocide-tolerance-of-salmonella-enterica-serovar-senftenberg-to-antimicrobial-susceptibility-serum-resistance-and-outer-membrane-proteins
#9
Bożena Futoma-Kołoch, Bartłomiej Dudek, Katarzyna Kapczyńska, Eva Krzyżewska, Martyna Wańczyk, Kamila Korzekwa, Jacek Rybka, Elżbieta Klausa, Gabriela Bugla-Płoskońska
A new emerging phenomenon is the association between the incorrect use of biocides in the process of disinfection in farms and the emergence of cross-resistance in Salmonella populations. Adaptation of the microorganisms to the sub-inhibitory concentrations of the disinfectants is not clear, but may result in an increase of sensitivity or resistance to antibiotics, depending on the biocide used and the challenged Salmonella serovar. Exposure of five Salmonella enterica subsp. enterica serovar Senftenberg (S...
July 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28694839/gene-expression-profiling-of-whole-blood-cells-supports-a-more-efficient-mitochondrial-respiration-in-hypoxia-challenged-gilthead-sea-bream-sparus-aurata
#10
Juan Antonio Martos-Sitcha, Azucena Bermejo-Nogales, Josep Alvar Calduch-Giner, Jaume Pérez-Sánchez
BACKGROUND: Acclimation to abiotic challenges, including decreases in O2 availability, requires physiological and anatomical phenotyping to accommodate the organism to the environmental conditions. The retention of a nucleus and functional mitochondria in mature fish red blood cells makes blood a promising tissue to analyse the transcriptome and metabolic responses of hypoxia-challenged fish in an integrative and non-invasive manner. METHODS: Juvenile gilthead sea bream (Sparus aurata) were reared at 20-21 °C under normoxic conditions (> 85% O2 saturation) followed by exposure to a gradual decrease in water O2 concentration to 3...
2017: Frontiers in Zoology
https://www.readbyqxmd.com/read/28693313/comparative-proteomic-analysis-of-the-outer-membrane-protein-43-omp43-deficient-bartonella-henselae
#11
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae
Outer membrane proteins (OMPs) of gram-negative bacteria constitute the first line of defense that protects the cells against environmental stresses including chemical, biophysical, and biological attacks. Although 43-kDa outer membrane protein (OMP43) is major porin protein among the Bartonella henselae-derived OMPs, its function remains unknown. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate function of OMP43. Interestingly, Δomp43 showed a weaker proliferative ability compared to wild type (WT) of B...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28678862/pseudomonas-aeruginosa-cells-attached-to-a-surface-display-a-typical-proteome-early-as-20-minutes-of-incubation
#12
Marc Crouzet, Stéphane Claverol, Anne-Marie Lomenech, Caroline Le Sénéchal, Patricia Costaglioli, Christophe Barthe, Bertrand Garbay, Marc Bonneu, Sébastien Vilain
Biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. Biofilms are associated with chronic infections. Controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. Thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from it would be possible to develop inhibitors against bacterial colonization and potentiate antibiotherapy...
2017: PloS One
https://www.readbyqxmd.com/read/28676795/electricity-generation-by-shewanella-decolorationis-s12-without-cytochrome-c
#13
Yonggang Yang, Guannan Kong, Xingjuan Chen, Yingli Lian, Wenzong Liu, Meiying Xu
Bacterial extracellular electron transfer (EET) plays a key role in various natural and engineering processes. Outer membrane c-type cytochromes (OMCs) are considered to be essential in bacterial EET. However, most bacteria do not have OMCs but have redox proteins other than OMCs in their extracellular polymeric substances of biofilms. We hypothesized that these extracellular non-cytochrome c proteins (ENCP) could contribute to EET, especially with the facilitation of electron mediators. This study compared the electrode respiring capacity of wild type Shewanella decolorationis S12 and an OMC-deficient mutant...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28676391/bcl-2-proteins-and-apoptosis-recent-insights-and-unknowns
#14
Frank Edlich
Proteins of the B-cell lymphoma-2 (BCL-2) family control the intrinsic apoptosis pathway. The pro-apoptotic BCL-2 proteins BAX and BAK can commit a cell to its programmed death by permeabilizing the outer mitochondrial membrane (OMM) and subsequent initiation of the caspase cascade. Therefore, the activities of BAX and BAK are precisely controlled by a complex network of proteins inside and outside the BCL-2 family. Cells survive by constant control of dynamic translocation and retrotranslocation of BAX and BAK to the mitochondria and back into the cytosol...
July 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28676336/quantitative-proteomic-analysis-of-iron-regulated-outer-membrane-proteins-in-aeromonas-hydrophila-as-potential-vaccine-candidates
#15
Yuqian Wang, Huarong Chen, Zhuang Guo, Lina Sun, Yuying Fu, Tao Li, Wenxiong Lin, Xiangmin Lin
The iron-regulated outer membrane protein (OMP) of Aeromonas hydrophila is an effective vaccine candidate, but its intrinsic functional components are largely unknown. In this study, we compared the differentially expressed sarcosine-insoluble fractions of A. hydrophila in iron-limited and normal medium using tandem mass tag labeling-based quantitative proteomics, and identified 91 upregulated proteins including 21 OMPs and 83 downregulated proteins including 10 OMPs. Subsequent bioinformatics analysis showed that iron chelate transport-related proteins were enriched in increasing abundance, whereas oxidoreductase activity and translation-related proteins were significantly enriched in decreasing abundance...
July 1, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28667599/identification-of-protein-secretion-systems-in-bacterial-genomes-using-macsyfinder
#16
Sophie S Abby, Eduardo P C Rocha
Protein secretion systems are complex molecular machineries that translocate proteins through the outer membrane, and sometimes through multiple other barriers. They have evolved by co-option of components from other envelope-associated cellular machineries, making them sometimes difficult to identify and discriminate. Here, we describe how to identify protein secretion systems in bacterial genomes using MacSyFinder. This flexible computational tool uses the knowledge stemming from experimental studies to identify homologous systems in genome data...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28666720/clinicopathological-implications-to-micropapillary-bladder-urothelial-carcinoma-of-the-presence-of-sialyl-lewis-x-decorated-mucin-1-in-stroma-facing-membranes
#17
Tomochika Shinagawa, Hitomi Hoshino, Minekatsu Taga, Yasuhiro Sakai, Yoshiaki Imamura, Osamu Yokoyama, Motohiro Kobayashi
OBJECTIVES: Bladder urothelial carcinoma (UC) comprises more than 90% of all bladder cancers. Among several UC variants, micropapillary UC (MPUC) is a rare one with high potential for lymphovascular invasion and subsequent lymph node metastasis. Histologically, MPUC is characterized by the presence of small papillary carcinoma cell clusters surrounded by lacunar spaces. Immunohistochemically, the outer circumference of these clusters, that is, the stroma-facing membrane of carcinoma cells, is reportedly almost invariably positive for mucin 1 (MUC1) protein and to a lesser extent for sialyl Lewis X (sLeX) carbohydrates; however, the clinicopathological implications of these expression patterns have not been fully investigated...
June 27, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28662203/detection-of-bacterial-reactive-natural-igm-antibodies-in-desert-bighorn-sheep-populations
#18
Brian S Dugovich, Melanie J Peel, Amy L Palmer, Ryszard A Zielke, Aleksandra E Sikora, Brianna R Beechler, Anna E Jolles, Clinton W Epps, Brian P Dolan
Ecoimmunology is a burgeoning field of ecology which studies immune responses in wildlife by utilizing general immune assays such as the detection of natural antibody. Unlike adaptive antibodies, natural antibodies are important in innate immune responses and often recognized conserved epitopes present in pathogens. Here, we describe a procedure for measuring natural antibodies reactive to bacterial antigens that may be applicable to a variety of organisms. IgM from desert bighorn sheep plasma samples was tested for reactivity to outer membrane proteins from Vibrio coralliilyticus, a marine bacterium to which sheep would have not been exposed...
2017: PloS One
https://www.readbyqxmd.com/read/28661222/expression-of-caspase-signaling-components-in-the-outer-membranes-of-chronic-subdural-hematomas
#19
Koji Osuka, Yasuo Watanabe, Nobuteru Usuda, Masahiro Aoyama, Kenichiro Iwami, Mikinobu Takeuchi, Takeya Watabe, Masakazu Takayasu
Chronic subdural hematoma (CSDH) is fundamentally treatable through surgery, although it recurs in some cases. We have observed several cases of spontaneous resolution of CSDH outer membranes, including in trabecular CSDH, after trepanation surgery. In this study, we examined the expression of molecules involved in caspase signaling in CSDH outer membranes. Eight patients whose outer membranes were successfully obtained during trepanation surgery were included in this study. The expression of Fas; Fas-associated death domain (FADD); tumor necrosis factor receptor type 1-associated death domain (TRADD); receptor-interacting protein (RIP); caspases 3, 7, 8 and 9; poly-(ADP-ribose) polymerase (PARP); DNA fragmentation factor 45 (DFF45) and β-actin was examined by Western blot analysis...
June 29, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28651566/arginine-kinase-from-haemonchus-contortus-decreased-the-proliferation-and-increased-the-apoptosis-of-goat-pbmcs-in-vitro
#20
Muhammad Ehsan, WenXiang Gao, Javaid Ali Gadahi, MingMin Lu, XinChao Liu, YuJian Wang, RuoFeng Yan, LiXin Xu, XiaoKai Song, XiangRui Li
BACKGROUND: Arginine kinase (AK), an important member of phosphagen kinase family has been extensively studied in various vertebrates and invertebrates. Immunologically, AKs are important constituents of different body parts, involved in various biological and cellular functions, and considered as immune-modulator and effector for pro-inflammatory cytokines. However, immunoregulatory changes of host cells triggered by AK protein of Haemonchus contortus, a parasitic nematode of ruminants, are still unknown...
June 26, 2017: Parasites & Vectors
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