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outer membrane protein analysis

Xu'ai Lin, Guohui Xiao, Dongjiao Luo, Liangliang Kong, Xu Chen, Dexter Sun, Jie Yan
BACKGROUND: Leptospirosis is an important reemerging zoonosis, with more than half a million cases reported annually, and is caused by pathogenic Leptospira species. Development of a universal vaccine is one of the major strategic goals to overcome the disease burden of leptospirosis. In this study, a chimeric multi-epitope protein-based vaccine was designed and tested for its potency to induce a specific immune response and provide protection against L. interrogans infection. RESULTS: The protein, containing four repeats of six T- and B-cell combined epitopes from the leptospiral outer membrane proteins, OmpL1, LipL32 and LipL21, was expressed and purified...
October 14, 2016: BMC Microbiology
Zhizhen Qi, Yujun Cui, Qingwen Zhang, Ruifu Yang
This chapter summarized the taxonomy and typing works of Yersinia pestis since it's firstly identified in Hong Kong in 1894. Phenotyping methods that based on phenotypic characteristics, including biotyping, serotyping, antibiogram analysis, bacteriocin typing, phage typing, and plasmid typing, were firstly applied in classification of Y. pestis in subspecies level. And then, with the advancement of molecular biological technology, the methods based on outer membrane protein profiles, fatty acid composition, and bacterial mass fingerprinting were also used to identify the populations within Y...
2016: Advances in Experimental Medicine and Biology
Paula Tucci, Verónica Estevez, Lorena Becco, Florencia Cabrera-Cabrera, Germán Grotiuz, Eduardo Reolon, Mónica Marín
Bovine Respiratory Disease is the most costly disease that affects beef and dairy cattle industry. Its etiology is multifactorial, arising from predisposing environmental stress conditions as well as the action of several different respiratory pathogens. This situation has hindered the development of effective control strategies. Although different type of vaccines are available, many currently marketed vaccines are based on inactivated cultures of the main viral and bacterial agents involved in this pathology...
September 2016: Heliyon
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: Periodontitis is a polymicrobial, chronic, and degenerative disease that can lead to destruction of the teeth-supporting tissues and eventually to loss of teeth. Aggregatibacter actinomycetemcomitans is predominantly associated with periodontal diseases. Outer membrane protein (Omp) 100 is a more important virulence factor of A. actinomycetemcomitans due to the effect of adhesion and invasion into human gingival epithelial cells. Attachment of A. actinomycetemcomitans inhibition is significant in the treatment process...
September 30, 2016: Photodiagnosis and Photodynamic Therapy
Bartłomiej Dudek, Eva Krzyżewska, Katarzyna Kapczyńska, Jacek Rybka, Aleksandra Pawlak, Kamila Korzekwa, Elżbieta Klausa, Gabriela Bugla-Płoskońska
Differential analysis of outer membrane composition of S. Enteritidis strains, resistant to 50% normal human serum (NHS) was performed in order to find factors influencing the resistance to higher concentrations of NHS. Ten S. Enteritidis clinical strains, resistant to 50% NHS, all producing very long lipopolysaccharide, were subjected to the challenge of 75% NHS. Five extreme strains: two resistant and three sensitive to 75% NHS, were chosen for the further analysis of outer membrane proteins composition. Substantial differences were found in the levels of particular outer membrane proteins between resistant and sensitive strains, i...
2016: PloS One
Yasuhiro Horibata, Hiromi Ando, Peixiang Zhang, Laurent Vergnes, Chieko Aoyama, Masahiko Itoh, Karen Reue, Hiroyuki Sugimoto
Phosphatidylcholine (PC) is a major phospholipid of mitochondria, composing 40-50% of both the outer and the inner membranes. However, PC must be imported from its production organelles because mitochondria lack the enzymes essential for PC biosynthesis. In a previous study, we found that StarD7 mediates the intracellular transfer of PC to mitochondria. Therefore, in this study, we analyzed the contribution of StarD7 to the maintenance of mitochondrial phospholipid content and function using siRNA-mediated knockdown (KD) and knockout (KO) of the StarD7 gene in HEPA-1 cells...
September 30, 2016: Journal of Biological Chemistry
Martin Lappann, Andreas Otto, Madita Brauer, Dörte Becher, Ulrich Vogel, Kay Johswich
Neisseria meningitidis, the meningococcus, bears the potential of life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx, Neisseria meningitidis must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37°C, the temperature commonly used in experimental settings. Here, we compared meningococcal biofilm formation, autoaggregation and cellular adherence between 32°C and 37°C and found a clear increase in all these phenotypes at 32°C, suggestive for a stronger in vivo colonization capability at this temperature...
September 26, 2016: Infection and Immunity
Kin Chan, Clifford Shone, J Richard Hesp
PURPOSE: The focus of this study was to characterise the effect of clinically relevant stress inducing conditions on the production and composition of proinflammatory outer membrane vesicles (OMVs) produced from ST131 ExPEC clinical isolates. EXPERIMENTAL DESIGN: A label-free method (SINQ) was used to identify changes in the respective OMV proteomes following exposure of the ExPEC strains to antibiotics and low iron. Nanoparticle tracking analysis was used to quantify changes in abundance and size of OMVs produced by the gentamicin resistant (GenR) and sensitive (GenS) ExPEC strains...
September 26, 2016: Proteomics. Clinical Applications
Ankita Kothari, Marimikel Charrier, Yu-Wei Wu, Stephanie Malfatti, Carol E Zhou, Steven W Singer, Larry Dugan, Aindrila Mukhopadhyay
The hydrocarbonoclastic bacterium Acinetobacter venetianus RAG-1 has attracted substantial attention due to its powerful oil degrading capabilities and its potential to play an important ecological role in the cleanup of alkanes. In this study we compare the transcriptome of the strain RAG-1 grown in dodecane, the corresponding alkanol (dodecanol), and sodium acetate for the characterization of genes involved in dodecane uptake and utilization. Comparison of the transcriptional responses of RAG-1 grown on dodecane led to the identification of 1074 genes that were differentially expressed relative to sodium acetate...
September 22, 2016: FEMS Microbiology Letters
Dong-Dong Di, Hai Jiang, Li-Li Tian, Jing-Li Kang, Wen Zhang, Xin-Ping Yi, Feng Ye, Qi Zhong, Bo Ni, You-Yu He, Lin Xia, Yao Yu, Bu-Yun Cui, Xiang Mao, Wei-Xing Fan
BACKGROUND: Brucellosis is a bacterial disease caused by Brucella infection. In the late fifties, Brucella suis vaccine strain S2 with reduced virulence was obtained by serial transfer of a virulent B. suis biovar 1 strain in China. It has been widely used for vaccination in China since 1971. Until now, the mechanisms underlie virulence attenuation of S2 are still unknown. RESULTS: In this paper, the whole genome sequencing of S2 was carried out by Illumina Hiseq2000 sequencing method...
2016: BMC Genomics
Wipa Suginta, Mathias Winterhalter, M F Smith
The protein channel chitoporin (ChiP), which is used by marine bacteria to translocate selected sugar molecules through the outer cell membrane, is studied via single channel current measurements in water and heavy water sugar solutions. The dynamic trapping and escape probabilities of sugar molecules from different monomers in the trimeric channel are characterized, including their dependence on channel orientation and sensitivity to a deuterium isotope effect. A detailed analysis of stochastic current fluctuations reveals that the trapping properties of chitoporin exhibit memory effects: the rate of trapping transitions depends on the previous sequence of transitions; and intermonomer correlations: the average trapping rate of an unblocked monomer is larger when its neighboring monomers are blocked...
September 14, 2016: Biochimica et Biophysica Acta
Huanhuan Liu, Qihong Li, Xiaozhuo Zhang, Zhongyu Li, Zedong Wang, Mingxin Song, Feng Wei, Shuchao Wang, Quan Liu
BACKGROUND: Tick-borne rickettsioses are considered important emerging zoonoses worldwide, but their etiological agents, rickettsiae, remain poorly characterized in northeastern China, where many human cases have been reported during the past several years. Here, we determined the characteristics of Rickettsia spp. infections in ticks in this area. METHODS: Ticks were collected by flagging vegetation from Jilin and Heilongjiang provinces of northeastern China followed by morphological identification...
2016: Parasites & Vectors
Dezhi Wu, Naoki Yamaji, Miki Yamane, Miho Kashino-Fujii, Kazuhiro Sato, Jian Feng Ma
The Natural Resistance Associated Macrophage Protein (Nramp) represents a transporter family for metal ions in all organisms. Here, we functionally characterized a member of Nramp family in barley (Hordeum vulgare), HvNramp5. This member showed different expression patterns, transport substrate specificity and cellular localization from its close homolog in rice (Oryza sativa), OsNramp5; although HvNramp5 was also localized to the plasma membrane. HvNramp5 was mainly expressed in the roots and its expression was not affected by Cd and deficiency of Zn, Cu and Mn, but slightly up-regulated by Fe deficiency...
September 12, 2016: Plant Physiology
Zuowei Wu, Balamurugan Periaswamy, Orhan Sahin, Michael Yaeger, Paul Plummer, Weiwei Zhai, Zhangqi Shen, Lei Dai, Swaine L Chen, Qijing Zhang
Infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. Association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. Campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. C. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of C. jejuni recently emerged, which is able to translocate across the intestinal tract, causing systemic infection and abortion in pregnant animals...
September 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
Abdelrahim Zoued, Chloé J Cassaro, Eric Durand, Badreddine Douzi, Alexandre P España, Christian Cambillau, Laure Journet, Eric Cascales
The Type VI secretion system (T6SS) is a multiprotein complex that delivers toxin effectors in both prokaryotic and eukaryotic cells. It is constituted of a long cytoplasmic structure - the tail - made of stacked Hcp hexamers and wrapped by a contractile sheath. Contraction of the sheath propels the inner tube capped by the VgrG spike protein towards the target cell. This tubular structure is built onto an assembly platform - the baseplate - that is composed of the TssEFGK-VgrG subunits. During the assembly process, the baseplate is recruited to a trans-envelope complex comprising the TssJ outer membrane lipoprotein and the TssL and TssM inner membrane proteins...
September 3, 2016: Journal of Molecular Biology
Jen-Jie Lee, Ying-Chen Wu, Chih-Jung Kuo, Shih-Ling Hsuan, Ter-Hsin Chen
The outer membrane protein TolC, which is one of the key components of several multidrug efflux pumps, is thought to be involved in various independent systems in Enterobacteriaceae. Since the acidic environment of the stomach is an important protection barrier against foodborne pathogen infections in hosts, we evaluated whether TolC played a role in the acid tolerance of Salmonella enterica serovar Choleraesuis. Comparison of the acid tolerance of the tolC mutant and the parental wild-type strain showed that the absence of TolC limits the ability of Salmonella to sustain life under extreme acidic conditions...
September 25, 2016: Veterinary Microbiology
Behzad Dehghani, Mohammad Mottamedifar, Hossein Khoshkharam-Roodmajani, Amir Hassanzadeh, Kamyar Zomorrodian, Amir Rahimi
BACKGROUND: Outer membrane proteins (OMPs) constitute the main structure and about half of the cell wall of Gram-negative bacteria. The OMPs of Escherichia coli (E. coli) play an important role in its drug resistance. Previous studies have shown that the OMPs of E. coli enhance its pathogenic effects by helping the bacterium to evade the immune defense and promote its adsorption to host cells. We sought to compare E. coli isolates collected from different hospital wards and to perform a primary investigation of the association between the serotypes and profiles of their OMPs...
September 2016: Iranian Journal of Medical Sciences
Tracy L Meehan, Sandy B Serizier, Sarah E Kleinsorge, Kimberly McCall
Programmed cell death (PCD) is essential for health and development. Generally, the last step of PCD is clearance, or engulfment, by phagocytes. Engulfment can be broken down into five basic steps: attraction of the phagocyte, recognition of the dying cell, internalization, phagosome maturation, and acidification of the engulfed material. The Drosophila melanogaster ovary serves as an excellent model to study diverse types of PCD and engulfment by epithelial cells. Here, we describe several methods to detect and analyze multiple steps of engulfment in the Drosophila ovary: recognition, vesicle uptake, phagosome maturation, and acidification...
2016: Methods in Molecular Biology
Yuan-Chih Mao, Chin-Lu Chang, Yhu-Chering Huang, Lin-Hui Su, Chao-Tai Lee
BACKGROUND: Providencia stuartii survives well in natural environment and often causes opportunistic infection in residents of long-term care facilities (LTCFs). Clinical isolates of P. stuartii are usually resistant to multiple antibiotics. The bacterium is also naturally resistant to colistin and tigecycline. Treatment of infections caused by carbapenem-resistant P. stuartii is challenging. METHODS: During a 15-month period in 2013-2014, four isolates (P1, P2, and P3B/P3U) of P...
July 29, 2016: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
Sascha Lange, W Trent Franks, Nandhakishore Rajagopalan, Kristina Döring, Michel A Geiger, Arne Linden, Barth-Jan van Rossum, Günter Kramer, Bernd Bukau, Hartmut Oschkinat
Proteins are synthesized in cells by ribosomes and, in parallel, prepared for folding or targeting. While ribosomal protein synthesis is progressing, the nascent chain exposes amino-terminal signal sequences or transmembrane domains that mediate interactions with specific interaction partners, such as the signal recognition particle (SRP), the SecA-adenosine triphosphatase, or the trigger factor. These binding events can set the course for folding in the cytoplasm and translocation across or insertion into membranes...
August 2016: Science Advances
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