keyword
MENU ▼
Read by QxMD icon Read
search

outer membrane protein analysis

keyword
https://www.readbyqxmd.com/read/28104494/thoughts-on-interactions-between-pgrmc1-and-diverse-attested-and-potential-hydrophobic-ligands
#1
REVIEW
Michael A Cahill, Amy E Medlock
Progesterone Receptor Membrane Component 1 (PGRMC1) is located in many different subcellular locations with many different attested and probably location-specific functions. PGRMC1 was recently identified in the mitochondrial outer membrane where it interacts with ferrochelatase, the last enzyme in the heme synthetic pathway. It has been proposed that PGRMC1 may act as a chaperone to shuttle newly synthesized heme from the mitochondrion to cytochrome P450 (cyP450) enzymes. Here we consider potential roles that PGRMC1 may play in transferring heme, and other small hydrophobic ligands such as cholesterol and steroids, between the hydrophobic compartment of the membrane lipid bilayer interior to aqueous proteins, and perhaps to the membranes of other organelles...
January 16, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28094338/mitochondrial-protein-import-functional-analysis-of-the-highly-diverged-tom22-orthologue-of-trypanosoma-brucei
#2
Jan Mani, Samuel Rout, Silvia Desy, André Schneider
The β-barrel protein Tom40 and the α-helically anchored membrane protein Tom22 are the only universally conserved subunits of the protein translocase of the mitochondrial outer membrane (TOM). Tom22 has an N-terminal cytosolic and a C-terminal intermembrane space domain. It occurs in two variants: one typified by the yeast protein which has a cytosolic domain containing a cluster of acidic residues, and a shorter variant typified by the plant protein that lacks this domain. Yeast-type Tom22 functions as a secondary protein import receptor and is also required for the stability of the TOM complex...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28077594/the-n-linking-glycosylation-system-from-actinobacillus-pleuropneumoniae-is-required-for-adhesion-and-has-potential-use-in-glycoengineering
#3
Jon Cuccui, Vanessa S Terra, Janine T Bossé, Andreas Naegeli, Sherif Abouelhadid, Yanwen Li, Chia-Wei Lin, Prerna Vohra, Alexander W Tucker, Andrew N Rycroft, Duncan J Maskell, Markus Aebi, Paul R Langford, Brendan W Wren
Actinobacillus pleuropneumoniae is a mucosal respiratory pathogen causing contagious porcine pleuropneumonia. Pathogenesis studies have demonstrated a major role for the capsule, exotoxins and outer membrane proteins. Actinobacillus pleuropneumoniae can also glycosylate proteins, using a cytoplasmic N-linked glycosylating enzyme designated NGT, but its transcriptional arrangement and role in virulence remains unknown. We investigated the NGT locus and demonstrated that the putative transcriptional unit consists of rimO, ngt and a glycosyltransferase termed agt...
January 2017: Open Biology
https://www.readbyqxmd.com/read/28070584/changes-in-protein-expression-profiles-in-bovine-endometrial-epithelial-cells-exposed-to-e-coli-lps-challenge
#4
Cristian Piras, Yongzhi Guo, Alessio Soggiu, Metasu Chanrot, Viviana Greco, Andrea Urbani, Gilles Charpigny, Luigi Bonizzi, Paola Roncada, Patrice Humblot
E. coli is one of the most frequently involved bacteria in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in pathogenic processes leading to post-partum metritis and endometritis in cattle. It also causes inflammation of the endometrium. The increase of cell proliferation by LPS is part of the inflammatory process. The aim of this study was to investigate possible changes in protein expression in relation to the proliferative response of bEECs after challenge with E...
January 10, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28069820/comprehensive-spatial-analysis-of-the-borrelia-burgdorferi-lipoproteome-reveals-a-compartmentalization-bias-toward-the-bacterial-surface
#5
Alexander S Dowdell, Maxwell D Murphy, Christina Azodi, Selene K Swanson, Laurence Florens, Shiyong Chen, Wolfram R Zückert
: The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, these lipoproteins assume varied roles. A prominent subset functioning as the apparent linchpins of the enzootic tick-vertebrate infection cycle has been explored as vaccine targets...
January 9, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28069648/the-acinetobacter-outer-membrane-contains-multiple-specific-channels-for-carbapenem-%C3%AE-lactams-as-revealed-by-kinetic-characterization-analyses-of-imipenem-permeation-into-acinetobacter-baylyi-cells
#6
Jorgelina Morán-Barrio, María M Cameranesi, Verónica Relling, Adriana S Limansky, Luciano Brambilla, Alejandro M Viale
The number and type of outer membrane (OM) channels responsible of carbapenem uptake in Acinetobacter are still not well defined. Here, we addressed these questions using Acinetobacter baylyi as a model species and a combination of methodologies aimed to characterize OM channels in their original membrane environment. Kinetic and competition analyses of imipenem (IPM) uptake by A. baylyi whole cells allowed us to identify different carbapenem-specific OM uptake sites. Comparative analyses of IPM uptake by A...
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28061865/proteomic-analysis-of-enterotoxigenic-escherichia-coli-etec-in-neutral-and-alkaline-conditions
#7
Lucia Gonzales-Siles, Roger Karlsson, Diarmuid Kenny, Anders Karlsson, Åsa Sjöling
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and travelers to endemic areas. Secretion of the heat labile AB5 toxin (LT) is induced by alkaline conditions. In this study, we determined the surface proteome of ETEC exposed to alkaline conditions (pH 9) as compared to neutral conditions (pH 7) using a LPI Hexalane FlowCell combined with quantitative proteomics. Relative quantitation with isobaric labeling (TMT) was used to compare peptide abundance and their corresponding proteins in multiple samples at MS/MS level...
January 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28054667/yersinia-pestis-halotolerance-illuminates-plague-reservoirs
#8
Maliya Alia Malek, Idir Bitam, Anthony Levasseur, Jérôme Terras, Jean Gaudart, Said Azza, Christophe Flaudrops, Catherine Robert, Didier Raoult, Michel Drancourt
The plague agent Yersinia pestis persists for years in the soil. Two millennia after swiping over Europe and North Africa, plague established permanent foci in North Africa but not in neighboring Europe. Mapping human plague foci reported in North Africa for 70 years indicated a significant location at <3 kilometers from the Mediterranean seashore or the edge of salted lakes named chotts. In Algeria, culturing 352 environmental specimens naturally containing 0.5 to 70 g/L NaCl yielded one Y. pestis Orientalis biotype isolate in a 40 g/L NaCl chott soil specimen...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28050585/dataset-of-the-proteome-of-purified-outer-membrane-vesicles-from-the-human-pathogen-aggregatibacter-actinomycetemcomintans
#9
Thomas Kieselbach, Jan Oscarsson
The Gram-negative bacterium Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen, which is linked to aggressive forms of periodontitis and can be associated with endocarditis. The outer membrane vesicles (OMVs) of this species contain effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA), which they can deliver into human host cells. The OMVs can also activate innate immunity through NOD1- and NOD2-active pathogen-associated molecular patterns. This dataset provides a proteome of highly purified OMVs from A...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/28040784/genetic-analysis-of-the-lambda-spanins-rz-and-rz1-identification-of-functional-domains
#10
Jesse Cahill, Manoj Rajaure, Chandler O'Leary, Jordan Sloan, Armando Marrufo, Ashley Holt, Aneesha Kulkarni, Oscar Hernandez, Ry Young
Coliphage lambda proteins Rz and Rz1 are the inner membrane and outer membrane subunits of the spanin complex, a heterotetramer that bridges the periplasm and is essential for the disruption of the outer membrane during phage lysis. Recent evidence suggests the spanin complex functions by fusing the inner and outer membrane. Here we use a genetics approach to investigate and characterize determinants of spanin function. Because Rz1 is entirely embedded in the +1 reading frame of Rz, the genes were dis-embedded before using random mutagenesis to construct a library of lysis-defective alleles for both genes...
December 30, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28035602/proteomic-characterization-of-the-outer-membrane-vesicle-of-the-halophilic-marine-bacterium-novosphingobium-pentaromativorans-us6-1
#11
Sung Ho Yun, Sang-Yeop Lee, Chi-Won Choi, Hayoung Lee, Hyun-Joo Ro, Sangmi Jun, Yong Min Kwon, Kae Kyoung Kwon, Sang-Jin Kim, Gun-Hwa Kim, Seung Il Kim
Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25-70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28031280/proteolytic-cleavage-of-the-immunodominant-outer-membrane-protein-rompa-in-rickettsia-rickettsii
#12
Nicholas F Noriea, Tina R Clark, David Mead, Ted Hackstadt
: Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated that rOmpB is proteolytically processed with the cleavage site residing near the autotransporter domain at the carboxy-terminal end of the protein, cleaving the 168 kDa precursor into apparent 120 kDa and 32 kDa fragments...
December 28, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/28026009/transcriptomic-profiling-of-platelet-senescence-and-platelet-extracellular-vesicles
#13
Annika Pienimaeki-Roemer, Tatiana Konovalova, Melina M Musri, Alexander Sigruener, Alfred Boettcher, Gunter Meister, Gerd Schmitz
BACKGROUND: Platelets (PLTs) are derived from megakaryocytes during PLT shedding. Senescent or activated PLTs are expanded in vascular and neurological diseases and release PLT extracellular vesicles (PL-EVs). A systematic analysis of regular messenger RNA (mRNA) and small RNA composition in PLTs and PL-EVs during in vitro PLT senescence has not yet been published. STUDY DESIGN AND METHODS: We isolated PLTs, total PL-EVs, and PL-EV subsets on Days 0 and 5 from human stored donor platelet concentrates...
January 2017: Transfusion
https://www.readbyqxmd.com/read/28018863/immunization-with-the-mipa-skp-or-etec_2479-antigens-confers-protection-against-enterotoxigenic-e-coli-strains-expressing-different-colonization-factors-in-a-mouse-pulmonary-challenge-model
#14
Michael P Hays, Amit Kumar, Francisco J Martinez-Becerra, Philip R Hardwidge
Achieving cross-protective efficacy against multiple bacterial strains or serotypes is an important goal of vaccine design. Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrheal disease in underdeveloped nations. We have been interested in identifying and characterizing ETEC antigens that generate protective immune responses independent of ETEC colonization factor (CF) expression. Our previous studies used proteomics to identify the ETEC MipA, Skp, and ETEC_2479 proteins as effective in protecting mice from homologous challenge with ETEC H10407 using a pulmonary inoculation model...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28018422/calcineurin-b-like-protein-cbl10-directly-interacts-with-toc34-translocon-of-the-outer-membrane-of-the-chloroplasts-and-decreases-its-gtpase-activity-in-arabidopsis
#15
Joo Hyuk Cho, Jeong Hwan Lee, Yoon Kook Park, Mi Na Choi, Kyung-Nam Kim
As calcium sensor relays in plants, calcineurin B-like (CBL) proteins provide an important contribution to decoding Ca(2+) signatures elicited by a variety of abiotic stresses. Currently, it is well known that CBLs perceive and transmit the Ca(2+) signals mainly to a group of serine/threonine protein kinases called CBL-interacting protein kinases (CIPKs). In this study, we report that the CBL10 member of this family has a novel interaction partner besides the CIPK proteins. Yeast two-hybrid screening with CBL10 as bait identified an Arabidopsis cDNA clone encoding a TOC34 protein, which is a member of the TOC (Translocon of the Outer membrane of the Chloroplasts) complex and possesses the GTPase activity...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28013396/transcriptomic-analysis-displays-the-effect-of-roemerine-on-the-motility-and-nutrient-uptake-in-escherichia-coli
#16
Dilara Ayyildiz, Kazim Yalcin Arga, Fatma Gizem Avci, Fatma Ece Altinisik, Caglayan Gurer, Gizem Gulsoy Toplan, Dilek Kazan, Katharina Wozny, Britta Brügger, Bulent Mertoglu, Berna Sariyar Akbulut
Among the different families of plant alkaloids, (-)-roemerine, an aporphine type, was recently shown to possess significant antibacterial activity in Escherichia coli. Based on the increasing demand for antibacterials with novel mechanisms of action, the present work investigates the potential of the plant-derived alkaloid (-)-roemerine as an antibacterial in E. coli cells using microarray technology. Analysis of the genome-wide transcriptional reprogramming in cells after 60 min treatment with 100 μg/mL (-)-roemerine showed significant changes in the expression of 241 genes (p value <0...
December 24, 2016: Current Genetics
https://www.readbyqxmd.com/read/28007883/evidence-for-amino-acid-snorkeling-from-a-high-resolution-in-vivo-analysis-of-fis1-tail-anchor-insertion-at-the-mitochondrial-outer-membrane
#17
Abdurrahman Keskin, Emel Akdoğan, Cory D Dunn
Proteins localized to mitochondria by a carboxyl-terminal tail anchor (TA) play roles in apoptosis, mitochondrial dynamics, and mitochondrial protein import. To reveal characteristics of TAs that may be important for mitochondrial targeting, we focused our attention upon the TA of the Saccharomyces cerevisiae Fis1 protein. Specifically, we generated a library of Fis1p TA variants fused to the Gal4 transcription factor, then, using next-generation sequencing, revealed which Fis1p TA mutations inhibited membrane insertion and allowed Gal4p activity in the nucleus...
December 22, 2016: Genetics
https://www.readbyqxmd.com/read/28004732/molecular-typing-and-virulence-analysis-of-multidrug-resistant-klebsiella-pneumoniae-clinical-isolates-recovered-from-egyptian-hospitals
#18
Reham Wasfi, Walid F Elkhatib, Hossam M Ashour
Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK efflux pump (32.14%) in the MDR isolates. FimH-1 and mrkD genes were prevalent in wound and blood isolates. FimH-1 gene was prevalent in sputum while mrkD gene was prevalent in urine...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28004290/quantitative-analysis-of-aquaporin-expression-levels-during-the-development-and-maturation-of-the-inner-ear
#19
Takushi Miyoshi, Taro Yamaguchi, Kiyokazu Ogita, Yasuko Tanaka, Ken-Ichi Ishibashi, Hiroaki Ito, Taisuke Kobayashi, Takayuki Nakagawa, Juichi Ito, Koichi Omori, Norio Yamamoto
Aquaporins (AQPs) are a family of small membrane proteins that transport water molecules across the plasma membrane along the osmotic gradient. Mammals express 13 subtypes of AQPs, including the recently reported "subcellular AQPs", AQP11 and 12. Each organ expresses specific subsets of AQP subtypes, and in the inner ear, AQPs are essential for the establishment and maintenance of two distinct fluids, endolymph and perilymph. To evaluate the contribution of AQPs during the establishment of inner ear function, we used quantitative reverse transcription polymerase chain reaction to quantify the expression levels of all known AQPs during the entire development and maturation of the inner ear...
December 21, 2016: Journal of the Association for Research in Otolaryngology: JARO
https://www.readbyqxmd.com/read/27997726/functional-relatedness-in-the-inv-mxi-spa-type-iii-secretion-system-family
#20
Jessica A Klein, Biren M Dave, Amogelang R Raphenya, Andrew G McArthur, Leigh A Knodler
Type III Secretion Systems (T3SSs) are structurally conserved nanomachines that span the inner and outer bacterial membranes, and via a protruding needle complex contact host cell membranes and deliver type III effector proteins. T3SS are phylogenetically divided into several families based on structural basal body components. Here we have studied the evolutionary and functional conservation of four T3SS proteins from the Inv/Mxi-Spa family: a cytosolic chaperone, two hydrophobic translocators that form a plasma membrane-integral pore, and the hydrophilic "tip complex" translocator that connects the T3SS needle to the translocon pore...
December 20, 2016: Molecular Microbiology
keyword
keyword
119647
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"