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https://www.readbyqxmd.com/read/28728289/-the-protective-effect-of-pigment-epithelial-derived-factor-modified-human-umbilical-cord-mesenchymal-stem-cells-on-rats-with-diabetic-retinopathy
#1
W Zhang, H T Duan, S Chen, Y X Wang, J H Kong, M Dong, X Bi, J Song
Objective: To investigate the effect of pigment epithelial-derived factor (PEDF) gene-modified human umbilical cord mesenchymal stem cells (MSC) on rats with diabetic retinopathy (DR). Methods: Experimental study. Human umbilical cord MSC were transfected by lentivirus packaging PEDF-MSC-green fluorescent protein (GFP) and GFP-MSC plasmid vectors, and the expression of PEDF and vascular endothelial growth factor (VEGF) was measured in the cell culture medium. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: normal control group (group A), DR control group (group B), phosphate-buffered saline (PBS) treated group (group C), GFP-MSC treated group (group D) and PEDF-MSC-GFP treated group (group E), with 10 rats in each group...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28727913/alpha-l-fucosidase-immunoassay-for-early-detection-of-hepatocellular-carcinoma
#2
Eric Waidely, Abdulrahman Obaid Al-Youbi, Abdulaziz Saleh Omar Bashammakh, Mohammad Soror El-Shahawi, Roger M Leblanc
Detection of alpha-L-fucosidase has been shown to have relevance in diagnosing hepatocellular carcinoma. Few assays have been developed to measure this enzyme, with most relying on colorimetric techniques involving the enzyme's kinetics. While these assays are facile and quick, the sensitivity is not always sufficient for early tumor detection. To improve upon previous assays for alpha-L-fucosidase, a fluorescence based immunoassay was produced implementing an alpha-L-fucosidase specific antibody (FUCA2). The immobilization of the alpha-L-fucosidase-specific antibody onto a quartz slide was investigated with several bioconjugation approaches and an immunoassay for detection of alpha-L-fucosidase was produced...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727780/comparative-analysis-of-signature-genes-in-prrsv-infected-porcine-monocyte-derived-cells-to-different-stimuli
#3
Laura C Miller, Damarius S Fleming, Xiangdong Li, Darrell O Bayles, Frank Blecha, Yongming Sang
Monocyte-derived DCs (mDCs) are major target cells in porcine reproductive and respiratory syndrome virus (PRRSV) pathogenesis; however, the plasticity of mDCs in response to activation stimuli and PRRSV infection remains unstudied. In this study, we polarized mDCs, and applied genome-wide transcriptomic analysis and predicted protein-protein interaction networks to compare signature genes involved in mDCs activation and response to PRRSV infection. Porcine mDCs were polarized with mediators for 30 hours, then mock-infected, infected with PRRSV strain VR2332, or a highly pathogenic PRRSV strain (rJXwn06), for 5 h...
2017: PloS One
https://www.readbyqxmd.com/read/28726727/in-vitro-quantified-determination-of-%C3%AE-amyloid-42-peptides-a-biomarker-of-neuro-degenerative-disorders-in-pbs-and-human-serum-using-a-simple-cost-effective-thin-gold-film-biosensor
#4
Yifan Dai, Alireza Molazemhosseini, Chung Chiun Liu
A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffered saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The bio-recognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K₄Fe(CN)₆ and K₃Fe(CN)₆. Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction...
July 20, 2017: Biosensors
https://www.readbyqxmd.com/read/28724571/comparison-of-recruitment-strategy-outcomes-in-the-national-children-s-study
#5
Christina H Park, Marianne Winglee, Jennifer Kwan, Linda Andrews, Mark L Hudak
BACKGROUND AND OBJECTIVES: In 2000, the US Congress authorized the National Institutes of Health to conduct a prospective national longitudinal study of environmental influences on children's health and development from birth through 21 years. Several recruitment methodologies were piloted to determine the optimal strategy for a main National Children's Study. METHODS: After an initial pilot recruitment that used a household enumeration strategy performed poorly, the National Children's Study Vanguard Study developed and evaluated the feasibility, acceptability, and cost of 4 alternate strategies to recruit a large prospective national probability sample of pregnant women and their newborn children...
July 19, 2017: Pediatrics
https://www.readbyqxmd.com/read/28722434/design-considerations-for-silica-particle-doped-nitric-oxide-releasing-polyurethane-glucose-biosensor-membranes
#6
Robert J Soto, Jonathon B Schofield, Shaylyn E Walter, Maggie J Malone-Povolny, Mark H Schoenfisch
Nitric oxide (NO)-releasing polymers have proven useful for improving the biocompatibility of in vivo glucose biosensors. Unfortunately, leaching of the NO donor from the polymer matrix remains a critical design flaw of NO-releasing membranes. Herein, a toolbox of NO-releasing silica nanoparticles (SNPs) was utilized to systematically evaluate SNP leaching from a diverse selection of biomedical-grade polyurethane sensor membranes. Glucose sensor analytical performance and NO-release kinetics from the sensor membranes were also evaluated as a function of particle and polyurethane (PU) chemistries...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28720771/plasmonically-enhanced-reflectance-of-heat-radiation-from-low-bandgap-semiconductor-microinclusions
#7
Janika Tang, Vaibhav Thakore, Tapio Ala-Nissila
Increased reflectance from the inclusion of highly scattering particles at low volume fractions in an insulating dielectric offers a promising way to reduce radiative thermal losses at high temperatures. Here, we investigate plasmonic resonance driven enhanced scattering from microinclusions of low-bandgap semiconductors (InP, Si, Ge, PbS, InAs and Te) in an insulating composite to tailor its infrared reflectance for minimizing thermal losses from radiative transfer. To this end, we compute the spectral properties of the microcomposites using Monte Carlo modeling and compare them with results from Fresnel equations...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720201/exposure-to-buffer-solution-alters-tendon-hydration-and-mechanics
#8
Babak N Safa, Kyle D Meadows, Spencer E Szczesny, Dawn M Elliott
A buffer solution is often used to maintain tissue hydration during mechanical testing. The most commonly used buffer solution is a physiological concentration of phosphate buffered saline (PBS); however, PBS increases the tissue's water content and decreases its tensile stiffness. In addition, solutes from the buffer can diffuse into the tissue and interact with its structure and mechanics. These bathing solution effects can confound the outcome and interpretation of mechanical tests. Potential bathing solution artifacts, including solute diffusion, and their effect on mechanical properties, are not well understood...
July 6, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28719617/microrna-155-deficiency-leads-to-decreased-autoantibody-levels-and-reduced-severity-of-nephritis-and-pneumonitis-in-pristane-induced-lupus
#9
Harald Leiss, Wilhelm Salzberger, Barbara Jacobs, Irina Gessl, Nicolas Kozakowski, Stephan Blüml, Antonia Puchner, Attila Kiss, Bruno K Podesser, Josef S Smolen, Georg H Stummvoll
OBJECTIVE: We herein examine the role of endogenous miR155 in the development of systemic manifestations in pristane induced lupus. MATERIALS AND METHODS: Systemic lupus in miR155-deficient and wild type mice was induced upon injection of pristane and analyzed after 8 months, PBS-injected mice served as controls. Glomerulonephritis and pneumonitis were quantified using the kidney biopsy score and a newly adapted histomorphometric image analysis system; lung tissue was further analyzed by tissue cytometry...
2017: PloS One
https://www.readbyqxmd.com/read/28719065/unconventional-route-to-uniform-hollow-semiconducting-nanoparticles-with-tailorable-dimensions-compositions-surface-chemistry-and-near-infrared-absorption
#10
Yanjie He, Xinchang Pang, Beibei Jiang, Chaowei Feng, Yeu Wei Harn, Yihuang Chen, Young Jun Yoon, Shuang Pan, Cheng-Hsin Lu, Yajing Chang, Mona Zebarjadi, Zhitao Kang, Naresh Thadhani, Juan Peng, Zhiqun Lin
Despite recent impressive advances in synthesizing lead chalcogenide solid nanoparticles, there are no demonstrations of lead chalcogenide hollow nanoparticles (HNPs) with controlled hollow diameter and shell thickness as current synthetic approaches for HNPs have inherent limitations associated with inability to precisely control the dimensions and restriction of applicable materials in scope. Herein, we report on an unconventional strategy for crafting uniform PbS and PbTe HNPs with tailorable size, surface chemistry, and near-IR absorption...
July 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28718290/enhanced-long-term-and-thermal-stability-of-polymer-solar-cells-in-air-at-high-humidity-with-the-formation-of-unusual-quantum-dot-networks
#11
Long Tan, Fan Yang, Mee Rahn Kim, Pandeng Li, Deepak Thrithamarassery Gangadharan, Joelle Margot, Ricardo Izquierdo, Mohamed Chaker, Dongling Ma
Due to the practical applications of polymer solar cells (PSCs), their stability recently has received increasing attention. Herein, a new strategy was developed to largely enhance the long-term and thermal stability of PSCs in air with a relatively high humidity of 50-60% without any encapsulation. In this strategy, semiconductor PbS/CdS core/shell quantum dots (QDs) were incorporated into the photoactive blend of poly(3-hexylthiophene) (P3HT) and phenyl-C61-butyric acid methyl ester (PCBM). By replacing the initial ligands of oleic acid with halide ligands on the surface of PbS/CdS QDs via solution-phase-ligand-exchange, we were able to form unusual, continuous QD networks in the film of P3HT:PCBM, which effectively stabilized the photoactive layer...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28717995/ovomucin-nanoparticles-promising-carriers-for-mucosal-delivery-of-drugs-and-bioactive-compounds
#12
Ali Akbari, Jianping Wu
Ovomucin, with a similar structure to mucin and gelation ability at room temperature, is a potential mucosal carrier. To evaluate this potential, the mucoadhesive property of ovomucin was studied by the tensile strength and rheology methods, and compared with three known mucoadhesive polymers, chitosan, polyacrylic acid (PAA), and alginate. Ovomucin particles were basically spherical in shape with an average size of ~572, 235 and 54 nm prepared at pH 5, 6.5, and 9, respectively. The absolute value of the zeta potential increased from ~ -51 mV at pH 5 to ~ -59 and -91 mV at pH 6...
July 17, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28716803/psychiatric-boarding-in-the-pediatric-inpatient-medical-setting-a-retrospective-analysis
#13
Katherine A S Gallagher, I Simona Bujoreanu, Priscilla Cheung, Christine Choi, Sara Golden, Kerry Brodziak, Gabriela Andrade, Patricia Ibeziako
BACKGROUND AND OBJECTIVES: Psychiatric concerns are a common presenting problem for pediatric providers across many settings, particularly on inpatient medical services. The volume of youth requiring intensive psychiatric treatment outnumbers the availability of psychiatric placements, and as a result many youth must board on pediatric medical units while awaiting placement. As the phenomenon of boarding in the inpatient pediatric setting increases, it is important to understand trends in boarding volume and characteristics of pediatric psychiatric boarders (PBs) and understand the supports they receive while boarding...
July 17, 2017: Hospital Pediatrics
https://www.readbyqxmd.com/read/28716746/controlled-release-and-anti-proliferative-effect-of-imatinib-mesylate-loaded-sporopollenin-microcapsules-extracted-from-pollens-of-betula-pendula
#14
Idris Sargin, Lalehan Akyuz, Murat Kaya, Gamze Tan, Talip Ceter, Kevser Yildirim, Seymanur Ertosun, Gozde Hatun Aydin, Muge Topal
Sporopollenin is a promising material for drug encapsulation due to its excellent properties; uniformity in size, non-toxicity, chemically and thermally resilient nature. Herein, morphologically intact sporopollenin microcapsules were extracted from Betula pendula pollens. Cancer therapeutic agent (imatinib mesylate) was loaded into the microcapsules. The encapsulation efficiency by passive loading technique was found to be 21.46%. Release behaviour of the drug from microcapsules was found to be biphasic, with an initial fast release followed by a slower rate of release...
July 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28715761/effects-of-lead-mineralogy-on-soil-washing-enhanced-by-ferric-salts-as-extracting-and-oxidizing-agents
#15
Jong-Chan Yoo, Sang-Min Park, Geun-Seok Yoon, Daniel C W Tsang, Kitae Baek
In this study, we evaluated the feasibility of using ferric salts including FeCl3 and Fe(NO3)3 as extracting and oxidizing agents for a soil washing process to remediate Pb-contaminated soils. We treated various Pb minerals including PbO, PbCO3, Pb3(CO3)2(OH)2, PbSO4, PbS, and Pb5(PO4)3(OH) using ferric salts, and compared our results with those obtained using common washing agents of HCl, HNO3, disodium-ethylenediaminetetra-acetic acid (Na2-EDTA), and citric acid. The use of 50 mM Fe(NO3)3 extracted significantly more Pb (above 96% extraction) from Pb minerals except PbSO4 (below 55% extraction) compared to the other washing agents...
July 12, 2017: Chemosphere
https://www.readbyqxmd.com/read/28714202/an-all-solution-based-hybrid-cmos-like-quantum-dot-carbon-nanotube-inverter
#16
Artem G Shulga, Vladimir Derenskyi, Jorge Mario Salazar-Rios, Dmitry N Dirin, Martin Fritsch, Maksym V Kovalenko, Ullrich Scherf, Maria A Loi
The development of low-cost, flexible electronic devices is subordinated to the advancement in solution-based and low-temperature-processable semiconducting materials, such as colloidal quantum dots (QDs) and single-walled carbon nanotubes (SWCNTs). Here, excellent compatibility of QDs and SWCNTs as a complementary pair of semiconducting materials for fabrication of high-performance complementary metal-oxide-semiconductor (CMOS)-like inverters is demonstrated. The n-type field effect transistors (FETs) based on I(-) capped PbS QDs (Vth = 0...
July 17, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28714022/platelet-poor-plasma-gel-combined-with-amnion-improves-the-therapeutic-effects-of-human-umbilical-cord%C3%A2-derived-mesenchymal-stem-cells-on-wound-healing-in-rats
#17
Lujun Yang, Jie Ma, Saiyang Gan, Shuli Chu, Martin Maldonado, Jianda Zhou, Lian Ma, Shijie Tang
The aim of the present study was to investigate the efficacy of human umbilical cord‑derived mesenchymal stem cell (HUMSCs) embedded in platelet poor plasma (PPP) gel combined with amnion (PPPA) in improving wound healing on Sprague‑Dawley (SD) rats. HUMSCs were cultured and labeled with chloromethylbenzamido‑1,1'‑dioctadecyl‑3,3,3'3'‑tetramethylindocarbocyanine perchlorate (CM‑DiI) on their third passage. The expression levels of growth factors of HUMSCs in PPPA were assessed by ELISA. Full‑thickness excisional skin wounds were induced in 36 male SD rats, which were treated with PPPA grafted with HUMSCs (PPPAC), PPPA, or HUMSC or PBS injection...
July 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713519/common-solvents-for-making-extraction-of-allergenic-proteins-from-plants-pollens-for-prick-tests-and-related-factors-a-technical-review
#18
REVIEW
Hassan Mansouritorghabeh, Farahzad Jabbari-Azad, Abdolreza Varasteh, Mojtaba Sankian, Reza Farid-Hosseini
Collecting information on influencing factors in developing consistent and high-quality extracts results in accurate diagnosis and effective treatment of type I allergy (IgE mediated). Furthermore, considering that a large number of allergens are currently in practice, any attempt to develop a more effective procedure for preparing extract may be useful. Nowadays, different saline solvents, temperature, incubation time, and PH are being incorporated for preparing allergen extracts. The objective of the current study was to clear and address the commonest of solvent buffers and allied conditions for making extracts of pollens of grasses, trees, and weeds...
May 2017: Electronic Physician
https://www.readbyqxmd.com/read/28711898/professional-burnout-syndrome-in-doctors-of-surgical-specialties-in-ukraine-causes-consequences-labor-optimization-ways
#19
Dmytro A Skurupii, Dmytro A Kholod, Evgen G Sonnik
INTRODUCTION: The professional burnout syndrome (PBS) affects quality of medical care provision for people, which is acquires the special actuality in terms of reforming the health care system. AIM: To study ways to improve the efficiency of doctors of surgical specialties based on analyzes of PBS and its consequences. MATERIALS AND METHODS: A survey of psychological tests and 62 surgical doctors was carried out. RESULTS: It was found out that the PBS reaches a peak after 11 to 15 years of working experience...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28708102/ability-of-soil-isolated-actinobacterial-strains-to-prevent-bind-and-biodegrade-ochratoxin-a
#20
Rachelle El Khoury, Florence Mathieu, Ali Atoui, Hiba Kawtharani, Anthony El Khoury, Charbel Afif, Richard G Maroun, André El Khoury
Ochratoxin A (OTA) is one of the most important mycotoxins, and contaminates several agricultural products, particularly cereals, grapes, maize, barley, spices and coffee. The aim of this project was to reduce the levels of OTA by supplementing the artificially contaminated solutions with seven strains of actinobacteria (AT10, AT8, SN7, MS1, ML5, G10 and PT1) in order to evaluate their capacity for binding and metabolizing the OTA, as well as their ability to reduce the expression of the genes responsible for its production in A...
July 14, 2017: Toxins
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