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Nancy Standart, Dominique Weil
P-bodies (PBs) are cytosolic RNP granules that are conserved among eukaryotic organisms. In the past few years, major progress has been made in understanding the biochemical and biophysical mechanisms that lead to their formation. However, whether they play a role in mRNA storage or decay remains actively debated. P-bodies were recently isolated from human cells by a novel fluorescence-activated particle sorting (FAPS) approach that enabled the characterization of their protein and RNA content, providing new insights into their function...
June 13, 2018: Trends in Genetics: TIG
Usman Riaz, Leela Rakesh, Ishraq Shabib, Waseem Haider
The issue of long-term incompatible interactions associated with the permanent implants can be eliminated by using various biodegradable metal implants. The recent research is focusing on the use of degradable stents to restore most of the hindrances of capillaries, and coronary arteries by supplying instant blood flow with constant mechanical and structural support. However, internal endothelialization and infection due to the corrosion of implanted stents are not easy to diagnose in the long run. In the recent past, magnesium (Mg) has been widely investigated for the cardiovascular stent applications...
June 5, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Yuanfeng Pan, Jingchun Wang, PingxiongCai, Huining Xiao
In this work, the stepwise synthesis of interpenetrating polymer network (IPN) strategy was developed in an attempt to fabricate novel hydrogels consisting of sugarcane bagasse cellulose (SBC), carboxymethylcellulose (CMC) and poly (N-isopropylacrylamide) (PNIPAm) as a dual-responsive drug carrier. The pretreated and dissolved SBC was pre-crosslinked using epichlorohydrin in the presence of CMC, followed by in-situ free-radical polymerization of NIPAm in the presence of N,N'-Methylene-bis(acrylamide) as a crosslinking agent...
June 13, 2018: International Journal of Biological Macromolecules
Brendon Wahlberg, Harmanvir Ghuman, Jessie R Liu, Michel Modo
Intracerebral implantation of cell suspensions is finding its clinical translation with encouraging results in patients with stroke. However, the survival of cells in the brain remains poor. Although the biological potential of neural stem cells (NSCs) is widely documented, the biomechanical effects of delivering cells through a syringe-needle remain poorly understood. We here detailed the biomechanical forces (pressure, shear stress) that cells are exposed to during ejection through different sized needles (20G, 26G, 32G) and syringes (10, 50, 250 µL) at relevant flow rates (1, 5, 10 µL/min)...
June 15, 2018: Scientific Reports
Nilesh Umakant Deshpande, Manickam Jayakannan
Biotin-conjugated multi-stimuli-responsive polysaccharide vesicular nanocarriers are designed and developed, for the first time, to accomplish receptor-mediated endocytosis in cancer cells and to deliver anticancer drugs at the intracellular compartments. For this purpose, a new renewable hydrophobic unit was custom designed with redox-degradable disulphide and enzyme-biodegradable aliphatic ester chemical linkages and it was conjugated along with biotin on the dextran backbone. The dextran derivative self-assembled into nanovesicles of < 200 nm in size which were characterized by dynamic and static light scattering, electron and atomic force microscopes...
June 15, 2018: Biomacromolecules
Chengliang Deng, Zhiyuan Liu, Yuanzhen Yao, Ruichi Liu, Zairong Wei, Dali Wang
Objective: To investigate the effect of human adipose-derived stem cells (hADSCs) on pressure ulcers in mouse. Methods: The subcutaneous adipose tissue from voluntary donation was harvested. Then the hADSCs were isolated and cultured by mechanical isolation combined with typeⅠcollagenase digestion. The 3rd generation cells were identified by osteogenic, adipogenic, chondrogenic differentiations and flow cytometry. The platelet rich plasma (PRP) from peripheral blood donated by healthy volunteers was prepared by centrifugation...
June 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
Manuel Toledano-Osorio, Jegdish P Babu, Raquel Osorio, Antonio L Medina-Castillo, Franklin García-Godoy, Manuel Toledano
Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis , Lactobacillus lactis , Streptoccocus mutans , gordonii and sobrinus were grown and the number of bacteria was determined by optical density. Nanoparticles were suspended in phosphate-buffered saline (PBS) at 10, 1 and 0...
June 14, 2018: Materials
Bo Hu, Nan Jiang, Qing Zhou, Sheng Cao, Shunji Gao, Binghong Zhang, Jinling Chen, Ruiqiang Guo
BACKGROUND: The thrombolysis in micro-circulation after acute myocardial infarction has been an unsolved issue, as elimination effect of acute thrombolysis and primary intervention were unsatisfied. Stable cavitation using acoustic phase-change nanoparticles may have potential for thrombolysis. Therefore, we sought to investigate a novel treatment method with dodecafluoropentane (DDFP) nanoparticles for rapid and effective thrombolysis in an in-vitro artificial vascular system, as a mimicking preparation of coronary circulation...
June 11, 2018: International Journal of Cardiology
Haruka Mizobuchi, Wataru Fujii, Shoko Isokawa, Kanna Ishizuka, Yihan Wang, Sayoko Watanabe, Chizu Sanjoba, Yoshitsugu Matsumoto, Yasuyuki Goto
Hepatic dysfunction is one of the clinical features in severe malaria. However, the mechanism of hepatic injury during malaria is still unknown. Myeloid-related protein (MRP) 14 is abundantly expressed by myeloid cells and involved in various inflammatory diseases. We previously reported that serum MRP14 is elevated in mice infected with Plasmodium berghei ANKA. In order to verify whether extracellular MRP14 is involved in the pathology of hepatic injury during rodent malaria, we intravenously administrated recombinant MRP14 (rMRP14) to mice infected with P...
2018: PloS One
Bernard Gottschalk
To find a deterministic pencil beam algorithm that computes, from first principles, the dose in some region of interest when a known heterogeneous terrain is irradiated by known proton beams.&#13; &#13; Methods: The terrain is discretized into slabs perpendicular to the nominal beam direction. Pencil beams (PBs) are transported through each slab using generalized Fermi-Eyges theory with the correct stopping and scattering powers for each material (no water-like assumption).&#13; &#13; At transverse heterogeneities PBs are divided into smaller ones using either redefinition (all PBs divided) or dynamic splitting (PBs divided one at a time as needed)...
June 14, 2018: Physics in Medicine and Biology
Marina Carvalho Prado, Nancy Kudsi de Carvalho, Rafael Pino Vitti, Fabrício Aulo Ogliari, Luciana Moura Sassone, Emmanuel João Nogueira Leal Silva
This study investigated the bond strength of two experimental root canal sealers based on MTA and butyl ethylene glycol disalicylate: MTAe and MTAe-HA. The reference materials used for comparison were AH Plus and MTA Fillapex. Twenty human upper incisors were selected and one 1 mm slice was obtained from the cervical third of each root. On the coronal surface of each slice, four 0.9 mm wide holes were drilled through the dentine. Standardized irrigation was performed and holes were filled with one of the four tested sealers: AH Plus, MTA Fillapex, MTAe, and MTAe-HA...
March 2018: Brazilian Dental Journal
Shigeko Kijimoto-Ochiai, Tokuko Matsumoto-Mizuno, Daisuke Kamimura, Masaaki Murakami, Miwako Kobayashi, Ichiro Matsuoka, Hiroshi Ochiai, Hideharu Ishida, Makoto Kiso, Keiko Kamimura, Toshiaki Koda
Membrane-bound sialidases in the mouse thymus are unique and mysterious because their activity at pH 6.5 is equal to or higher than that in the acidic region. The pH curve like this has never been reported in membrane-bound form. To clarify this enzyme, we studied the sialidase activities of crude membrane fractions from immature-T, mature-T and non-T cells from C57BL/6 mice and from SM/J mice, a strain with a defect in NEU1 activity. Non-T cells from C57BL/6 mice had high activity at pH 6.5, but those from SM/J mice did not...
May 1, 2018: Glycobiology
Tao Yan, Poornima Venkat, Michael Chopp, Alex Zacharek, Peng Yu, Ruizhuo Ning, Xiaoxi Qiao, Mark R Kelley, Jieli Chen
APX3330 is a selective inhibitor of APE1/Ref-1 redox activity. In this study, we investigate the therapeutic effects and underlying mechanisms of APX3330 treatment in type one diabetes mellitus (T1DM) stroke rats. Adult male Wistar rats were induced with T1DM and subjected to transient middle cerebral artery occlusion (MCAo) and treated with either PBS or APX3330 (10mg/kg, oral gavage) starting at 24h after MCAo, and daily for 14 days. Rats were sacrificed at 14 days after MCAo and, blood brain barrier (BBB) permeability, ischemic lesion volume, immunohistochemistry, cell death assay, Western blot, real time PCR, and angiogenic ELISA array were performed...
June 2018: Aging and Disease
Tugrul Kaymak, Ercan Koca, Mustafa Atak, Ercan Sarikaya, Joerg Stroka
Background: Tarhana is a traditional fermented, sun-dried Turkish food containing yogurtand cereals. There are several potential sources of mycotoxins in tarhana, such as contamination of ingredients or formation during preparation, when water activity is suitable for fungal growth and may lead to mycotoxin production during fermentation or subsequent sun-drying. Objective: To optimize an immunoaffinity column method and carry out single-laboratory validation for the determination of aflatoxins B1 , B₂, G1 , and G₂ together with ochratoxin A (OTA) in tarhana...
June 12, 2018: Journal of AOAC International
Carolina Assis P Vilela, Lucas Eduardo B Souza, Rubens C Siqueira, Rodrigo T Calado, Dimas T Covas, Jayter S Paula
BACKGROUND: Bone marrow-derived mesenchymal stromal cell (MSC) therapy is a promising treatment for several degenerative ocular diseases; however, no reproducible method of monitoring these cells into the eye has been established. The aim of this study was to describe successful bioluminescence imaging (BLI) to detect viable luciferase-expressing MSC in the eye. METHODS: Human donor MSC in culture were transduced with 50 μl luciferase lentiviral vector (three viral particles/cell) prior to intraocular injection...
June 13, 2018: Stem Cell Research & Therapy
J Zheng, H Xue, H Zhang, L Wang, L Zheng, X Wang, X Lin, S Jin, J Wu
Hepatitis B virus (HBV) infection is a major public health problem and immune tolerance is responsible for persistent HBV infection. HBV therapeutic vaccines targeting HBV e antigen (HBeAg) may have an excellent effect in overcoming HBV immune tolerance. Thus, there is urgency for designing therapeutic vaccine candidates that target HBeAg. In this research, we fused the C (472-507) gene sequence of HBV with the extracellular domain of human CD40 ligand sequence and ligated this fused sequence into the pEGFP-N1 vector to construct the recombinant plasmid pEGFP-N1-C (472-507)-ecdCD40L...
2018: Acta Virologica
Nichole M Sell, Rob Turrisi, Nichole M Scaglione, Michael J Cleveland, Kimberly A Mallett
Heavy drinking is a risk factor for sexual assault. Although protective behavioral strategies (PBS) tend to be associated with reduced alcohol consumption, there are studies showing differential benefits for using these strategies. The current study extended the research on PBS and drinking by examining daily associations between alcohol consumption and sexual assault PBS (e.g., letting others know one's whereabouts) versus stopping or limiting drinking PBS (e.g., planning to stop drinking at a predetermined time) and manner of drinking PBS (e...
March 2018: Psychology of Women Quarterly
Shahindokht Bassiri-Jahromi, Mohammad Reza Pourshafie, Esmat Mirabzade Ardakani, Amir Hooshang Ehsani, Aida Doostkam, Farzad Katirae, Ehsan Mostafavi
Background: The pomegranate peel extract is a rich source of natural antioxidant and antimicrobial activity. The aim of the present investigation was to evaluate the in vivo antifungal activity of the pomegranate peel extract and to compare it with nystatin against oral candidiasis in Wistar rats. Methods: Thirty-five male Wistar rats, 6 to 8 weeks old and 220 to 250 g in weight, were used for animal studies. The rats were randomly divided into 7 groups. All the rats, except the control group, were immunosuppressed with cyclosporine (40 mg/kg/d) and hydrocortisone acetate (500 µg/kg/d)...
May 2018: Iranian Journal of Medical Sciences
Mingxi Zhang, Jingying Yue, Ran Cui, Zhuoran Ma, Hao Wan, Feifei Wang, Shoujun Zhu, Ying Zhou, Yun Kuang, Yeteng Zhong, Dai-Wen Pang, Hongjie Dai
With suppressed photon scattering and diminished autofluorescence, in vivo fluorescence imaging in the 1,500- to 1,700-nm range of the near-IR (NIR) spectrum (NIR-IIb window) can afford high clarity and deep tissue penetration. However, there has been a lack of NIR-IIb fluorescent probes with sufficient brightness and aqueous stability. Here, we present a bright fluorescent probe emitting at ∼1,600 nm based on core/shell lead sulfide/cadmium sulfide (CdS) quantum dots (CSQDs) synthesized in organic phase...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Yong-Kun Wan, Hui-Hui Li, Lin Zuo, Xiao-Li Wang, Li-Yuan Wang, Wen-Xin He, Hui Jiang, Shou-Xiang Wang, Jie Sheng, Min Zhang, Hai-Chun Qian, Fang-Fang Yang, Hong Xie, Shi-Fang Gao, Qiang Fang, Xiao-di Yang, Mu-Ling Liu
OBJECTIVE: To observe the effect of Schistosoma japonicum cysteine protease inhibitor (rSjCystatin) for treatment of lipopolysaccharide (LPS)-induced sepsis in mice. METHODS: After a week of adaptive feeding, 54 BALB/c mice were randomly divided into normal control group (group A), sepsis group (group B), and rSjCystatin intervention group (group C). The mice in group A received an intraperitoneal injection of PBS (100 µL), and those in groups B and C were injected with PBS (100 µL) containing LPS (10 mg/kg); the mice in group C were also intraperitoneally injected with 25 µg sjCystatin in 100 µL PBS 30 min after LPS injection...
May 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
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